A large number of pathogens, especially fungi affect several eucalypt species from the nursery phase to the adult crops. Among fungal diseases, Mycosphaerella leaf disease is considered one of the main conditions, to which Eucalyptus globulus, is one of the most susceptible species. This disease is caused by several species into the genera Teratosphaeria and Mycosphaerella, and Teratosphaeria nubilosa is the most importance species. The aim of the present study was to verify the presence of T. nubilosa in materials collected in: Bagé-RS, Pedras Altas-RS, Botucatu-SP, Jacareí-SP and Itapeva-SP. By means of isolations from E. globulus leaves presenting cha racter istic symptoms of T. nubilosa, isolates of the fungus could be observed as to ascospore germination pattern and mycelial growth. The pathogen specie was determined by PCR using primers ITS1 and ITS4 and DNA sequencing, and submission to GenBank. From materials collected in all five studied places, confirming the presence of the agent of Mycosphaerella leaf disease in some of E. globulus crops in the South and Southeast regions of Brazil.
eucalyptus; leaf disease; Teratosphaeria nubilosa
