Abstract Erythema multiforme is a mucocutaneous disease characterized by the acute appearance of blisters and ulcers, with or without oral involvement. In this report, a 12-year-old female patient presented with extensive ulcerative lesions on the upper and lower lips, with painful symptoms and areas of bleeding and suppuration. Despite these lesions being restricted to the lips and skin around, the patient was diagnosed with erythema multiforme. Dexamethasone was then prescribed, in addition to the use of lip balm. At the first return evaluation, there was no regression of the clinical picture, and the medication was replaced by prednisone, associated with amoxicillin and 0.12% chlorhexidine mouthwash. In the second return visit, there was considerable regression of the lesion, and the process of gradual discontinuation of the corticosteroid was initiated. After 1 week, the lesions had healed, and the drug was discontinued. The oral cavity may be the only site affected by erythema multiforme, so knowledge of this disease is necessary for its correct diagnosis and treatment.

Resumo O eritema multiforme é uma doença mucocutânea, caracterizada pela ocorrência súbita de bolhas e úlceras, com ou sem envolvimento da cavidade oral. No presente relato, uma paciente do sexo feminino, com 12 anos de idade, apresentou lesões ulceradas extensas nos lábios, superior e inferior, acompanhadas de sintomas dolorosos, zonas de hemorragia e supuração. Embora essas lesões estivessem localizadas apenas nos lábios e na pele circundante, a paciente recebeu o diagnóstico de eritema multiforme. Foi então prescrita dexametasona, juntamente com a indicação de protetor labial. Na primeira avaliação de retorno, não houve regressão do estado clínico, resultando na substituição do medicamento por prednisona, em combinação com amoxicilina e colutório de clorexidina a 0,12%. Na segunda consulta de acompanhamento, houve regressão considerável da lesão, iniciando-se, assim, o processo gradual de suspensão do corticosteroide. Após 1 semana, as lesões cicatrizaram e o medicamento foi interrompido. A cavidade oral pode ser o único local afetado pelo eritema multiforme, sendo necessário o conhecimento dessa doença para o seu correto diagnóstico e tratamento.

Abstract Cyclodextrins (CDs) have the ability to encapsulate numerous molecules and have applicability in several industrial areas, however, their cost has made their use difficult. To seek alternatives that may enable the use of DCs, the present study evaluated the efficiency of the ultrafiltration process in a continuous system to produce CDs from the enzyme cyclomaltodextrin glucanotransferase (CGTase) from recombinant Bacillus subtilis WB800. The possibility of using the crude enzyme as an alternative means of producing CDs was also evaluated. All strategies evaluated in this research proved to be promising for the production of CDs, with the production of β-CD being the most efficient (average of 15 mmol/L) using crude recombinant enzyme and a temperature of 50 °C. Therefore, the results obtained can contribute to the reduction of stages and costs of production of CDs, favoring their industrial application.

ABSTRACT Purpose To analyze the association between hearing loss and health vulnerability in children aged 25 to 36 months. Methods Analytical observational cross-sectional study conducted through child hearing screening in nine day-care centers. The screening consisted of anamnesis, otoscopy, tympanometry, transient otoacoustic emissions, and pure tone audiometry. For each exam performed, the 'pass' and 'fail' criteria were established. The children's residential addresses were georeferenced and a choropleth map of the spatial distribution was built, considering the Health Vulnerability Index (HVI). The analysis of the association between the HVI and the variables sex, auditory assessment, and region area of the household was performed using Pearson's Chi-square and Fisher's Exact tests. Results Ninety-five children of both sexes were evaluated, of which 44.7% presented alterations in at least one of the exams performed, being referred for otorhinolaryngological evaluation and subsequent auditory assessment. Of the observed changes, 36.9% occurred in the tympanometry and 7.8% in the transient otoacoustic emissions. Among children referred for reassessment, 9.7% were diagnosed with conductive hearing loss, 13.6% results within normal limits and 21.4% did not attend for assessment. Of the children who presented the final diagnosis of conductive hearing loss (9.7%), 1.9% were classified as low-risk HVI and 6.8% as medium-risk HVI. There was statistical significance between HVI and the child's place of residence. Conclusion The association between hearing loss and HIV was not statistically significant; however, it was possible to observe that 77.7% of the children with hearing loss resided in sectors with medium- risk HIV

RESUMO Objetivo Analisar a associação entre perda auditiva e a vulnerabilidade à saúde em crianças na faixa etária de 25 a 36 meses. Método Estudo observacional analítico do tipo transversal realizado por meio da triagem auditiva infantil em nove creches. A triagem constou de anamnese, meatoscopia, timpanometria, emissões otoacústicas transientes e audiometria tonal limiar. Para cada exame realizado foi estabelecido o critério de “passa” e “falha”. Os endereços residenciais das crianças foram georreferenciados e foi construído mapa coroplético da distribuição espacial, considerando o Índice de Vulnerabilidade à Saúde (IVS). Foi realizada análise de associação entre o IVS com as variáveis sexo, exames audiológicos e regional de domicílio por meio dos testes Qui-quadrado de Pearson, e Exato de Fisher. Resultados Foram avaliadas 95 crianças de ambos os sexos, destas, 44,7% apresentaram alteração em pelo menos um dos exames realizados, sendo encaminhadas para avaliação otorrinolaringológica e auditiva. Das alterações observadas 36,9% ocorreram na timpanometria e 7,8% nas emissões otoacústicas transientes. Dentre crianças encaminhadas para avaliação, 9,7% apresentaram diagnóstico de perda auditiva do tipo condutiva, 13,6% resultados dentro da normalidade e 21,4% não compareceram para reavaliação. Das crianças que apresentaram o diagnóstico final de perda auditiva do tipo condutiva (9,7%), 1,9% foi classificado como IVS de risco baixo e 6,8% como IVS de risco médio. Houve significância estatística entre IVS e o local de residência da criança. Conclusão Não houve associação com significância estatística entre alteração auditiva e IVS, entretanto foi possível observar que 77,7% das crianças com diagnóstico de perda auditiva residiam em setores censitários de risco médio do IVS.

Resumo O fibroblastoma desmoplásico (FD) é um tumor benigno raro de partes moles de origem fibroblástica e miofibroblástica e sua ocorrência na cavidade oral é rara. Este estudo detalha um novo caso de uma lesão elevada no rebordo alveolar de uma mulher de 59 anos, dolorosa à mastigação. O exame histopatológico mostrou proliferação de células de morfologia fusiformes a estreladas, não encapsulada, em um estroma rico em colagénio e hipocelular. As células tumorais foram forte e difusamente positivas para vimentina e algumas células foram focalmente positivas para α-actina do músculo liso, CD99 e β-catenina. Os exames foram conclusivos para o diagnóstico de FD. O paciente está no período de acompanhamento e nenhuma lesão recorrente foi detetada em um ano. O FD descrito neste relato, apesar de apresentar características clínico-patológicas-imuno-histoquímicas semelhantes aos casos descritos anteriormente, é apresentado em um local oral incomum e associado a sintomatologia dolorosa. A imuno-histoquímica é uma ferramenta importante nos casos de FD nos quais o exame histopatológico não é conclusivo.

Abstract The desmoplastic fibroblastoma (DF) is a rare benign soft-tissue tumor of fibroblastic and myofibroblastic origin, and its occurrence in the oral cavity is rare. This study details a new case of an elevated lesion in the alveolar ridge of a 59-year-old woman, painful during mastication. The histopathological exam showed non-encapsulated stellate-to-spindle cells proliferation in a hypocellular collagen-rich stroma. Tumor cells were strongly and diffusely positive for vimentin, and some cells were focally positive for α-smooth muscle actin, CD99, and β-catenin. The exams were conclusive for DF diagnosis. The patient is in the follow-up period, and no recurrent lesions were detected within one year. Despite having similar clinical, pathological, and immunohistochemical features to the cases previously described, the DF in this report is at an unusual oral site and associated with painful symptomatology. Immunohistochemistry is an important tool in DF cases where the histopathological examination is not conclusive.

Resumo A pandemia provocada pelo novo coronavírus tem causado impactos nas interações sociais e nos hábitos de vida, incluindo mudanças no contexto da alimentação. Com o intuito de promover educação em saúde, diversas cartilhas sobre alimentação e nutrição foram elaboradas para a população. Este estudo, de abordagem qualitativa, realizou uma análise reflexiva de quatro cartilhas sobre alimentação e nutrição disponibilizadas durante a pandemia de covid-19, por meio da análise de conteúdo, e identificou três eixos temáticos: (1) planejamento das refeições; (2) higienização das compras e alimentos; e (3) alimentação saudável e escolha dos alimentos. As cartilhas analisadas orientam sobre aspectos práticos da alimentação e nutrição nesse período de incertezas em relação à saúde. Contudo, observou-se uma lacuna na abordagem de temas englobando a saúde integral do indivíduo como: segurança alimentar e aspectos culturais, simbólicos e macrossociais relacionados às escolhas alimentares.

Abstract The pandemic caused by the new coronavirus has impacted social interactions and lifestyle habits, including changes in the context of food. To promote health education, several food and nutrition guides were developed for the population. This study, of qualitative approach, carried out a reflexive analysis of four food and nutrition guides made during the pandemic, by content analysis, and identified three thematic axes: (1) meal planning; (2) cleaning of purchases and food; and (3) healthy eating food choice. The analyzed guides provide guidance on practical aspects of food and nutrition in this period of uncertainty regarding health protection. However, the approach to themes encompassing the individual’s integral health such as: food security and cultural, symbolic, and macrosocial aspects related to food choices, showed a gap.

RESUMO Objetivo avaliar o efeito da variação da intensidade de estimulação sobre as respostas das emissões otoacústicas produto de distorção em indivíduos com perda auditiva neurossensorial, utilizando um protocolo de gradiente de fase das emissões. Métodos estudo observacional transversal. Participaram 38 indivíduos com diagnóstico de perda auditiva neurossensorial de grau leve, moderado ou severo. Foram realizadas anamnese, meatoscopia, audiometria tonal liminar, logoaudiometria, imitanciometria, emissões otoacústicas produto de distorção e emissões otoacústicas residuais. As emissões otoacústicas residuais foram coletadas com o equipamento Echodia, modelo Elios®. O protocolo utilizado permite a variação dos parâmetros frequência e intensidade e as respostas são analisadas por meio do teste do Gradiente de Fase. As respostas registradas nas emissões residuais foram consideradas como “presente”, “ausente” e “artefato”, considerando a variação da fase em função de f1. Resultados Foram incluídas 72 orelhas. Houve diferença estatisticamente significativa nas frequências de 1300 Hz e 2000 Hz, ao comparar os resultados das emissões residuais. Ao correlacionar o resultado da audiometria e a intensidade de estimulação que evocou a emissão residual, houve correlação positiva para as frequências de 1000 Hz e 4000Hz. O “artefato” foi registrado, principalmente, nas frequências mais agudas: 56,2% em 3000 Hz e 58,2% em 4000 Hz. A emissão otoacústica residual presente foi registrada em 18,6% em 1000 Hz, 13,4% em 2000 Hz, 6,3% em 3000 Hz e 7,5% em 4000 Hz. Conclusão o aumento da intensidade de estimulação no exame de emissões pode auxiliar no estudo das células ciliadas residuais, desde que seja utilizado um protocolo capaz de diferenciar respostas fisiológicas de artefatos.

ABSTRACT Purpose To study the effect of stimulation intensity variation on the responses of distortion products in subjects with sensorineural hearing loss using a new protocol to register the otoacoustic emissions. Methods This is a cross-sectional observational study. The following procedures were performed: anamnesis, otoscopy, pure tone audiometry, speech audiometry, tympanometry, distortion product and residual otoacoustic emissions. The residual DPOAE were collected with the Echodia equipment, Elios®. The protocol that was developed allows the variation of frequency and intensity parameters and the responses are analyzed by phase gradient test. Responses recorded in residual otoacoustic emissions were considered “present”, “absent” or “artifact”. Results The total included ears was 72. On residual otoacoustic emissions test, at a frequency of 1300Hz and 2000Hz, there was statistically significant difference. By analyzing the average found in the audiometry and the results of residual emissions, only the frequency of 1300Hz showed a statistically significant association in all groups. By correlating the results of the audiometry and the stimulation intensity used to evoke the residual emission, there was positive correlation for the frequencies of 1000Hz and 4000Hz. The “artifact” was mostly recorded in the higher frequencies: 56.2% in 3000Hz and 58.2% in 4000 Hz. Residual EOAPD present was recorded as 18.6% at 1000Hz, 13.4% at 2000Hz, 6.3% at 3000Hz and 7.5% at 4000Hz. Conclusion The increased stimulation intensity in the otoacoustic emissions test can aid in the study of residual outer hair cells, as long as a protocol is used to check the correctness of the responses.

Resumo A pandemia provocada pelo novo coronavírus tem causado impactos nas interações sociais e nos hábitos de vida, incluindo mudanças no contexto da alimentação. Com o intuito de promover educação em saúde, diversas cartilhas sobre alimentação e nutrição foram elaboradas para a população. Este estudo, de abordagem qualitativa, realizou uma análise reflexiva de quatro cartilhas sobre alimentação e nutrição disponibilizadas durante a pandemia de covid-19, por meio da análise de conteúdo, e identificou três eixos temáticos: (1) planejamento das refeições; (2) higienização das compras e alimentos; e (3) alimentação saudável e escolha dos alimentos. As cartilhas analisadas orientam sobre aspectos práticos da alimentação e nutrição nesse período de incertezas em relação à saúde. Contudo, observou-se uma lacuna na abordagem de temas englobando a saúde integral do indivíduo como: segurança alimentar e aspectos culturais, simbólicos e macrossociais relacionados às escolhas alimentares.

Abstract The pandemic caused by the new coronavirus has impacted social interactions and lifestyle habits, including changes in the context of food. To promote health education, several food and nutrition guides were developed for the population. This study, of qualitative approach, carried out a reflexive analysis of four food and nutrition guides made during the pandemic, by content analysis, and identified three thematic axes: (1) meal planning; (2) cleaning of purchases and food; and (3) healthy eating food choice. The analyzed guides provide guidance on practical aspects of food and nutrition in this period of uncertainty regarding health protection. However, the approach to themes encompassing the individual’s integral health such as: food security and cultural, symbolic, and macrosocial aspects related to food choices, showed a gap.

Abstract Neuropathic pain (NP) affects more than 8% of the global population. The proposed action of the transient receptor potential ankyrin 1 (TRPA1) as a mechanosensor and the characterization of the transient receptor potential melastatin 8 (TRPM8) as a cold thermosensor raises the question of whether these receptors are implicated in NP. Our study aimed to evaluate the involvement of TRPA1 and TRPM8 in cold and mechanical signal transduction to obtain a comparative view in rat models of streptozotocin-induced diabetes (STZ) and chronic constriction injury of the sciatic nerve (CCI). The electronic von Frey test showed that STZ rats presented mechanical allodynia that was first evidenced on the 14th day after diabetes confirmation, and four days after CCI. This phenomenon was reduced by the intraplantar (ipl) administration of a TRPA1 receptor antagonist (HC-030031; 40 μL/300 μg/paw) in both NP models. Only CCI rats displayed cold hyperalgesia based on the cold plate test. The pharmacological blocking of TRPA1 through the injection of the antagonist attenuated cold hyperalgesia in this NP model. STZ animals showed a reduction in the number of flinches induced by the intraplantar injection of mustard oil (MO; TRPA1 agonist; 0.1%/50 μL/paw), or intraplantar injection of menthol (MT; TRPM8 agonist; 0.5% and 1%/50 μL/paw). The response induced by the ipl administration of MT (1%/50 µL/paw) was significantly different between the CCI and SHAM groups. Together, these data suggest a different pattern in nociceptive behavior associated with different models of NP, suggesting a variant involvement of TRPA1 and TRPM8 in both conditions.

Abstract Vasculitis is a group of several clinical conditions in which the main histopathological finding is fibrinoid necrosis in the walls of blood vessels. This article assesses the main dermatological aspects relevant to the clinical and laboratory diagnosis of small- and medium-vessel cutaneous and systemic vasculitis syndromes. The most important aspects of treatment are also discussed.

Abstract The term vasculitis refers to the inflammation of vessel walls. It may range in severity from a self-limited disorder in one single organ to a life-threatening disease due to multiple organ failure. It has many causes, although they result in only a few histological patterns of vascular inflammation. Vessels of any type and in any organ can be affected, a fact that results in a broad variety of signs and symptoms. Different vasculitides with indistinguishable clinical presentations have quite different prognosis and treatments. This condition presents many challenges to physicians in terms of classification, diagnosis, appropriate laboratory workup, and treatment. Moreover, it compels a careful follow-up. This article reviews the Chapel-Hill 2012 classification, etiology, recent insights in pathophysiology, some important dermatological clues for the diagnosis and summarizes treatment of some of these complex vasculitis syndromes.

Abstract Background: Macular lymphocytic arteritis most commonly presents as hyperpigmented macules on the lower limbs. The pathogenesis of this disease is still unclear and there is an ongoing debate regarding whether it represents a new form of cutaneous vasculitis or an indolent form of cutaneous polyarteritis nodosa. Objective: To describe clinical, histopathological, and laboratory findings of patients with the diagnosis of macular lymphocytic arteritis. Methods: A retrospective search was conducted by reviewing cases followed at the Vasculitis Clinic of the Dermatology Department, School of Medicine, University of São Paulo, between 2005 and 2017. Seven patients were included. Results: All cases were female, aged 9-46 years, and had hyperpigmented macules mainly on the legs. Three patients reported symptoms. Skin biopsies evidencing a predominantly lymphocytic infiltrate affecting arterioles at the dermal subcutaneous junction were found, as well as a typical luminal fibrin ring. None of the patients developed necrotic ulcers, neurological damage, or systemic manifestations. The follow-up ranged from 18 to 151 months, with a mean duration of 79 months. Study limitations: This study is subject to a number of limitations: small sample of patients, besides having a retrospective and uncontrolled study design. Conclusions: To the best of the authors’ knowledge, this series presents the longest duration of follow-up reported to date. During this period, none of the patients showed resolution of the lesions despite treatment, nor did any progress to systemic vasculitis. Similarities between clinical and skin biopsy findings support the hypothesis that macular lymphocytic arteritis is a benign, incomplete, and less aggressive form of cutaneous polyarteritis nodosa.

Resumo: A alface é uma das principais espécies de hortaliças utilizadas na produção orgânica, porém, os estudos e pesquisas na área de orgânicos são considerados escassos. O objetivo do estudo foi avaliar a produtividade e qualidade de sementes de genótipos de alface produzidas em dois anos consecutivos no sistema de manejo orgânico. Foram instalados dois plantios em condições de campo aberto correspondendo às estações de outono/inverno em 2016 e 2017. Avaliaram-se 10 genótipos de alface, em quatro repetições, utilizando biofertilizante suíno no manejo e colheita manual das sementes. As avaliações efetuadas foram: produtividade das sementes em kg.ha−1; teste físico (peso de 1000 sementes); teste de germinação e de vigor (primeira contagem de germinação, índice de velocidade de germinação e massa seca de plântulas). O genótipo Cubana e os dois genótipos Grand Rapids apresentaram produtividades superiores nas condições testadas. Em 2017 foram registrados maiores pesos de sementes. Os dois genótipos Grand Rapids atingiram o padrão mínimo de germinação estabelecido para a comercialização (80%). Vanda e AC 5058 apresentaram melhores desempenho para IVG e primeira contagem, e, Cinderela para massa seca de plântulas. Tais variedades mostraram ser promissoras para o cultivo orgânico.

Abstract: is one of the main vegetable species used in organic production. However, studies and research in the organic area are scarce. This study aimed to evaluate the yield and quality of seeds of lettuce genotypes produced in two consecutive years under an organic management system. Two plantations were installed under open field conditions corresponding to autumn/winter in 2016 and 2017. Ten lettuce genotypes were evaluated in four replications using a biofertilizer made from swine manure in the management and manual harvesting of seeds. Evaluations consisted of seed yield (in kg.ha−1), physical test (1000-seed weight), and germination and vigor test (first germination count, germination rate index, and seedling dry matter). The genotype Cubana and two Grand Rapids had higher yields under the tested conditions. Higher seed weights were recorded in 2017. Both genotypes Grand Rapids reached the minimum germination standard established for commercialization (80%). Vanda and AC 5058 showed a better performance for germination rate index and first germination count, while Cinderela had a better seedling dry matter. These varieties were promising for organic cultivation.

Abstract The panniculitides remain as one of the most challenging areas for clinicians, as they comprise a heterogeneous group of inflammatory diseases involving the subcutaneous fat with potentially-shared clinical and histopathological features. Clinically, most panniculitides present as red edematous nodules or plaques. Therefore, in addition to a detailed clinical history, a large scalpel biopsy of a recent-stage lesion with adequate representation of the subcutaneous tissue is essential to specific diagnosis and appropriate clinical management. Herein we review the panniculitides of particular interest to the rheumatologist.

RESUMO O estudo da descarga de sólidos de uma determinada bacia hidrográfica é importante para que se possam tomar decisões corretas quanto ao planejamento de gestão dos recursos hídricos. O objetivo do presente trabalho foi determinar curvas-chave que representam cargas de sedimentos em suspensão no Rio Piranhas. O estudo foi realizado na sub-bacia hidrográfica do Rio Piranhas. Foram realizadas 15 campanhas de medições hidrossedimentométricas, no período de novembro de 2012 a maio de 2013, envolvendo as medições de concentração de sedimentos em suspensão e de descargas líquida e sólida. Foram plotadas curvas-chave de sedimentos em suspensão para os períodos seco e chuvoso, as quais apresentaram bons coeficientes de determinação.

ABSTRACT The study of solid discharge of a hydrographic basin is important for making correct decisions regarding planning the management of water resources. The objective of the present study was to determine key planning curves that represented loads of suspended sediment in the River Piranhas. The study was carried out in the hydrographic sub-basin of Piranhas River. A total of 15 campaigns of hydrosedimentometric measurements was performed, in the period from November 2012 to May 2013, including the measurements of suspended sediment concentration and liquid and solid discharges. Key curves were plotted for sediment in suspension, for the dry and rainy periods, which presented good coefficients of determination.

Objetivou-se avaliar agronomicamente cinco acessos de mangarito nas condições de Montes Claros-MG. O experimento foi conduzido em Horta Experimental do Instituto de Ciências Agrárias da UFMG, Montes Claros, de 10/06/2010 a 03/06/2011. Empregou-se o delineamento de blocos casualizados, com cinco tratamentos e quatro repetições. Os tratamentos consistiram em acessos de mangarito cedidos pela Universidade Federal de Viçosa (Viçosa 1, Viçosa 2 e Viçosa 3) e pela Universidade Federal da Grande Dourados (Dourados), além do acesso local (Montes Claros). A parcela foi composta de quatro fileiras com 2,5 m de comprimento, com as plantas espaçadas de 0,5x0,5 m. Foram avaliados a produtividade total, de cormos, de cormelos e de cormelos comerciais, massa fresca e seca de cormelos comerciais, número de cormelos comerciais por planta e a produtividade de cormelos nas classes (com base no menor diâmetro) grande (≥30 mm), média (20-29 mm), pequena (10-19 mm) e refugo (<10 mm). Os acessos Viçosa 1, Montes Claros, Viçosa 2 e Viçosa 3 apresentaram as melhores respostas agronômicas quanto à produtividade total e de cormelos. Os componentes primários com as maiores correlações positivas com a produtividade comercial foram produtividade de cormelos classe média (0,9543**), número de cormelos comerciais por planta (0,8242**) e massa fresca de cormelos comerciais (0,8242**).

This study aimed to evaluate five accessions of tannia. The experiment was carried out in field at the Universidade Federal de Montes Claros, Minas Gerais State, Brazil, from June 2010 to June 2011. The experimental design consisted of randomized blocks with five treatments and four replications, a total of 20 plots. Treatments consisted of five accessions of tannia obtained at Universidade Federal de Viçosa (Viçosa 1, Viçosa 2 and Viçosa 3), Universidade Federal da Grande Dourados (Dourados), and a local accession (Montes Claros). The experimental plot comprised four rows spaced 0.5 m apart, with 2.5 m in length. The distance between plants in the row was 0.5 m. The agronomical characteristics evaluated were yield of corms, cormels, marketable cormels and total, fresh and dry weight of marketable cormels, number of marketable cormels per plant and the cormel yield of classes (based on the smallest diameter): large (≥30 mm), medium (20-29 mm), small (10-19 mm) and noncommercial (<10 mm). The Viçosa 1, Montes Claros, Viçosa 2 and Viçosa 3 accessions presented higher values of total yield and cormels. Primary components presenting high positive correlation in comparison to marketable yield were: yield of medium cormels (0.9543**), marketable cormels fresh weight (0.8242**) and number of marketable cormels per plant (0.8242**).