ABSTRACT L-carnitine perform a major role in transporting long-chain fatty acids into the mitochondria, where they are oxidized. It has been used in animal diets to decrease fat and increase muscle protein. The aim of this study was to evaluate the zootechnical performance, degree of steatosis in the liver, and genotoxic potential in Astyanax lacustris fed with different levels of L-carnitine (LC). Yellowtail tetra juveniles (n = 140) were distributed in 20 tanks of 70 L, with seven fish in each, in a water recirculation system with controlled temperature (27±0.1⁰C). The treatments with different levels of L-carnitine supplementation were: 0 (control), 250, 500, 750, and 1000 mg of LC per kg of food. The diets were provided twice a day for 60 days. The results showed that the different levels of LC did not affect (P>0.05) weight gain, survival, viscerosomatic index, and the liver hepatocytes showed a normal appearance. However, the use of LC supplementation showed genotoxic potential with a significant difference (P<0.05) for cell alterations when compared to the control at concentrations above 500mg kg-1.

RESUMO A L-carnitina exerce um papel importante no transporte de ácidos graxos de cadeia longa até a mitocôndria para serem oxidados e tem sido incorporada em rações para animais com o objetivo de diminuir a deposição de gordura e aumentar a proteína muscular. O objetivo deste trabalho foi avaliar o desempenho zootécnico, o grau de esteatose no fígado e o potencial genotóxico em Astyanax lacustris alimentados com diferentes níveis de L-carnitina (LC). Juvenis de lambari-do-rabo-amarelo (n=140) foram distribuídos em 20 caixas de 70L, sete peixes em cada, em um sistema de recirculação de água com temperatura controlada (27±0,1⁰C). Os tratamentos com os níveis de suplementação foram: 0 (controle), 250, 500, 750 e 1000 mg de LC kg-1 de ração. As dietas foram fornecidas duas vezes ao dia, durante 60 dias. Os resultados mostraram que os diferentes níveis de LC não influenciaram (P>0,05) o ganho de peso; a sobrevivência, o índice viscerossomático e os hepatócitos do fígado apresentaram-se com aparência normal. No entanto, a suplementação com LC apresentou potencial genotóxico com diferença significativa (P<0,05) para alterações celulares quando comparada ao controle em concentrações superiores a 500mg kg-1.

RESUMO A produção artesanal de queijos envolve uma tradição secular no estado de Minas Gerais, no Brasil, exercendo importante papel histórico e social. Objetivou-se, com este estudo, avaliar as características de queijos minas artesanal de sete regiões certificadas para sua produção em relação à sua composição físico-química e centesimal, a fim de se identificarem parâmetros que sejam úteis para diferenciá-los. A composição físico-química e centesimal revelou diferença entre os queijos das diferentes regiões para as variáveis nitrogênio solúvel, índices de extensão e profundidade de maturação, cinzas, oxidação, gordura e pH. Os maiores valores para os índices de extensão e profundidade de maturação foram observados na região do Cerrado. Os queijos da região da Canastra se mostraram diferentes daqueles das demais regiões em razão do maior teor de umidade, assim como os queijos de Campo das Vertentes, que apresentaram maiores valores de pH e compostos nitrogenados. Apesar das semelhanças entre os queijos em cada região, esses apresentaram posicionamentos dispersos na análise de componentes principais. Existem diferenças na composição físico-química e centesimal entre os queijos minas artesanais oriundos das distintas regiões em Minas Gerais, e os parâmetros analisados podem ser utilizados para diferenciá-los. Os teores de cinzas, gordura, índice de oxidação, teores de nitrogênio solúvel e pH foram os parâmetros que estiveram associados a maiores diferenças dos queijos conforme sua região de origem. Gerais Brasil social Objetivouse, Objetivouse Objetivou se, Objetivou-se estudo físicoquímica físico química diferenciálos. diferenciálos diferenciá los. los diferenciá-los cinzas oxidação Cerrado umidade Vertentes nitrogenados principais origem

ABSTRACT Artisanal cheese production involves a centuries-old tradition in the state of Minas Gerais in Brazil, playing an important historical and social role. The aim of this study was to evaluate the characteristics of artisanal Minas cheeses from seven regions certified for their production in relation to their physical-chemical and centesimal composition to identify parameters that are useful to differentiate them. There were differences among the cheeses from different regions for the soluble nitrogen variables, extension and depth of ripening indexes, ash, oxidation, fat and pH. The highest values for the ripening length and depth index were observed in the Cerrado region. The cheeses from the Canastra region were different due to the higher moisture content, and the cheeses from Campo das Vertentes presented higher pH and nitrogen compound values. Despite the similarities among cheeses in each region, they had dispersed positions in the principal components analysis. There are differences in the physicochemical and centesimal composition among the artisanal Minas cheeses from the distinct regions in Minas Gerais, and the analyzed parameters can be used to differentiate them. The contents of ash, fat, oxidation index, soluble nitrogen and pH were the parameters that were associated with greater differences in cheeses. centuriesold centuries old Brazil role physicalchemical physical chemical them variables indexes ash content analysis

Abstract This study aimed to develop and evaluate bread with the use of pulp and flours of pequi, in partial replacement of water and wheat flour, to develop a bakery product with good technological, nutritional and sensorial qualities. The pequi husk and pulp flours were obtained by means of a thermal pre-treatment, oven drying and standardization of the dry material. Whereas, the bread formulation was defined through the baker’s formulation. Besides, the dehydration process caused significant changes (p<0.05) in the L* value and chromaticity (C*), mainly of the flours (husk and pequi pulp), such changes are due to non-enzymatic oxidative processes and pigment degradation, especially carotenoids. The effect of the substitution of ingredients (wheat flour and water) by husk and pulp flours and pequi pulp contributed to the increase in lipid, crude fiber, nitrogen-free extract and energy value content. However, the substitution promoted changes in the attributes of color and textural properties, such as increased hardness, chewiness and cohesiveness. Nevertheless, all formulations showed good sensory acceptance and thus, pequi sweet breads can be implemented in school meals for contributing and meeting the nutritional recommendations established by the School Feeding Brazilian Program (PNAE).

ABSTRACT: The parasitic fauna of Podocnemididae species is of great relevance to understanding the ecological relationships of the organisms in this genus. The parasites can have a symbiotic relationship with their hosts; however, environmental imbalances may change this relationship. Therefore, these parasites may become agents that generate zoonoses, especially in animals used by parasites as a food source. The aim of this study was to investigate the gastrointestinal parasitic fauna of specimens of Podocnemis unifilis. The animals were collected from the Tocantins and Javaés Rivers, both of which flow in the State of Tocantins, predominantly in the Cerrado territory. Samples of stomach and intestinal contents were fixed in 10% formalin buffered with sodium phosphate, transferred to 70% alcohol for analysis, and separated after 72 hours. Parasites were isolated and identified according to morphology and identification key. Four species of parasites were observed in 100% of specimens of P. unifilis, namely Nematophila grandis (Trematoda) and Paratractis hystrix, Paraorientatractis semiannulata, and Orientatractis leiperi (Nematoda). N. grandis was the only parasite that was not observed in the intestine; it appeared to adhere to the inner wall of the stomach. All nematodes were observed both in the stomach and intestine. These data expand the knowledge related to the P. unifilis parasitic fauna and the geographic distribution of these parasites in P. unifilis from the Tocantins and Javaés Rivers.

RESUMO: A fauna parasitológica das espécies de Podocnemididae é de grande relevância para o conhecimento das relações ecológicas desse grupo de vertebrados. Os parasitos podem ocorrer em simbiose com os seus hospedeiros sem causar danos, mas o desequilíbrio ambiental pode causar um desequilíbrio nessa relação e, assim, serem agentes geradores de zoonoses, especialmente em animais que são utilizados como recurso alimentar. Esta pesquisa fez o levantamento da fauna parasitária gastrointestinal de espécimes de Podocnemis unifilis. Os animais foram coletados no rio Tocantins e Javaés, ambos no Estado do Tocantins, em território predominante de Cerrado. As amostras de conteúdo estomacal e intestinal foram fixadas em formol a 10% tamponado em fosfato de sódio, transferidas para álcool 70% para análise e separação após 72 horas. Os parasitos foram separados e identificados de acordo com morfologia e chave de identificação. Foram encontradas quatro espécies de parasitos com prevalência em 100% dos espécimes de P. unifilis, sendo elas Nematophila grandis (Trematoda) e Paratractis hystrix, Paraorientatractis semiannulata e Orientatractis leiperi (Nematoda). Somente N. grandis não foi observado no intestino, sendo encontrado somente aderido à parede interna do estômago. Todos os nematoides foram observados no estômago e intestino. Esses dados ampliam o conhecimento sobre a fauna parasitária de P. unifilis, e aumentam o conhecimento sobre a distribuição geográfica desses parasitos em P. unifilis dos Rios Tocantins e Javaés.

Islet transplantation represents a therapeutic option for type 1 diabetes (T1D). Long-term viability of transplanted islets requires improvement. Mesenchymal stromal cells (MSCs) have been proposed as adjuvants for islet transplantation facilitating grafting and functionality. Stem cell aggregation provides physiological interactions between cells and enhances the in situ concentration of modulators of inflammation and immunity. We established a hanging-drop culture of adult human skin fibroblast-like cells as spheroids, and skin spheroid-derived cells (SphCs) were characterized. We assessed the potential of SphCs in improving islet functionality by cotransplantation with a marginal mass of allogeneic islets in an experimental diabetic mouse model and characterized the secretome of SphCs by mass spectrometry-based proteomics. SphCs were characterized as multipotent progenitors and their coculture with anti-CD3 stimulated mouse splenocytes decreased CD4+ T cell proliferation with skewed cytokine secretion through an increase in the Th2/Th1 ratio profile. SphCs-conditioned media attenuated apoptosis of islets induced by cytokine challenge in vitro and importantly, intratesticular SphCs administration did not show tumorigenicity in immune-deficient mice. Moreover, SphCs improved glycemic control when cotransplanted with a marginal mass of allogeneic islets in a diabetic mouse model without pharmacological immunosuppression. SphCs' protein secretome differed from its paired skin fibroblast-like counterpart in containing 70% of up- and downregulated proteins and biological processes that overall positively influenced islets such as cytoprotection, cellular stress, metabolism, and survival. In summary, SphCs improved the performance of transplanted allogeneic islets in an experimental T1D model, without pharmacological immunosuppression. Future research is warranted to identify SphCs-secreted factors responsible for islets' endurance.

RESUMO Este estudo teve por objetivo identificar a prevalência e os principais fatores de risco relacionados com a transmissão da cisticercose bovina em animais enviados para abate e provenientes de propriedades localizadas na microrregião de Uberlândia, Minas Gerais, Brasil. As propriedades foram previamente avaliadas quanto à ocorrência de cisticercose durante a inspeção post mortem nos 12 meses antecedentes ao início da pesquisa; aquelas que revelaram animais com cisticercose bovina pelo menos uma vez durante esse período foram consideradas positivas. Foi realizado um estudo de corte transversal em 87 propriedades, onde foram coletadas 1024 amostras de soro bovino. O diagnóstico sorológico foi realizado por triagem pelo teste ELISA indireto, e os soros positivos foram confirmados pelo Immunoblot. A prevalência identificada neste estudo foi de 5,1% (IC 95%= 3,74-6,42). Os fatores de risco identificados foram: a origem dos bovinos (RC=4,9), o pastejo (RC=6,4) e o destino do esgoto da propriedade (RC=3,6). Esses fatores ambientais encontrados sugerem que medidas de controle do saneamento e restrição a pastagens além do limite da propriedade possam auxiliar na prevenção da doença na área de estudo.

ABSTRACT The aim of this study was to identify the prevalence and the main risk factors related to the transmission of bovine cysticercosis based on tracking animals sent for slaughter and coming from properties located in the micro-region of Uberlândia, Minas Gerais, Brazil. The properties were previously evaluated for the occurrence of cysticercosis during post-mortem inspection in the 12 months prior to the beginning of the research, and those with animals with bovine cysticercosis found at least once during this period were considered positive. A cross-sectional study was carried out on 87 properties, from which 1024 bovine serum samples were collected. Indirect ELISA performed serological diagnosis and Immunoblot confirmed positive sera. The prevalence found in this study was 5.1% (95% CI = 3.74-6.42). The risk factors identified were cattle origin (RC = 4.9), grazing (RC = 6.4) and sewage destination on the property (RC = 3.6). These environmental factors suggest that sanitation control measures and the restriction of pastures beyond the property boundary can help prevent disease in the study area. A control system based on risk analysis was discussed and proposed as a strategy to control bovine cysticercosis in the Triângulo Mineiro region and other regions of the country.

Abstract Fibrinolytic enzymes are considered promising alternative in the treatment of cardiovascular diseases by preventing fibrin clots. A protease from Mucor subtilissimus UCP 1262 was obtained by solid state fermentation and purified by ion exchange chromatography. The purified extract was administered at an acute dose of 2000 mg/mL to evaluate its toxic effects to the lungs of mice. After 14 days of treatment, a histomorphometric study was performed by the type 1 and 2 pneumocyte count and the evaluation of the lung area. As result, the experimental group showed a significant decrease of type 2 pneumocyte and although a decrease in the alveolar area was observed in relation to the control group, no significant pulmonary toxicity, emphysema, and fibrosis characteristics were detected. The in vitro tests suggest possible clinical applications for the enzyme.

Abstract Objective: The aim of this study was to analyze the relationship between body adiposity and physical fitness with performance in the Supine-to-Stand test (STS-test) in sedentary adolescents. Methods: Sixty-two adolescents, of both sexes, between 10 and 16 years old, participated in the study. Body mass (BM), height, waist circumference (WC), fat mass (FM), fat-free mass (FFM), right and left handgrip strength (HGS-right, HGS-left), abdominal resistance (ABDO), flexibility (FLEX), and cardiorespiratory fitness (VO2peak) were measured. Body mass index (BMI), z-score BMI (BMI-z), tri-ponderal mass index (TMI) and waist-to-height ratio (WHtR) were calculated. The STS-test was applied to evaluate the STS-MC by the movement patterns in the execution of the test. The STS-time in seconds (s) was categorized into terciles: fast (FG < 2.0 s), intermediate (IG = 2.0-2.6 s) and slow (SG > 2.6 s). One-way ANOVA, Chi-square, Spearman’s correlation coefficient as well as non-parametric tests were used, with significance p 0.05. Results: The SG presented higher BMI, BMI-z, TMI, WHtR, FM, %FM, as well as lower averages for %FFM, HGS-right, HGS-left, FLEX, ABDO, VO2peak, VO2peak relative to BM (VO2peakBM) in relation to GF. The BMI, BMI-z, TMI, WC, WHtR and FM showed moderate and direct correlations with STS-time and inverse with STS-MC (p < 0.01). HGS-right, HGS-left, ABDO, and VO2peakBM showed moderate and an inverse correlation with STS-time (p < 0.05). The VO2peakBM was moderate and with direct correlations to STS-MC (p < 0.01). Conclusion: It is concluded that excess fat and low physical fitness hamper STS-test performance. Therefore, the STS-test can be used for screening students to assess MC.

RESUMO O objetivo deste estudo foi avaliar a aplicação tópica do extrato alcoólico da semente e da casca da Dipteryx alata Vogel na cicatrização de feridas cutâneas, em camundongos. Um total de 54 camundongos C57BL/6 foram utilizados neste estudo, distribuídos em três grupos de 18 animais (controle, semente e casca). Em todos os animais, um fragmento de pele foi ressecado da região dorsal do tórax utilizando-se instrumento de punção de 9mm de diâmetro, após o qual foi realizada aplicação tópica de creme base (veículo), extrato hidroalcoólico 10% de semente ou casca, duas vezes ao dia. As avaliações macroscópica, histológica e imuno-histoquímica foram realizadas no sétimo, 14º e 21º dias de pós-operatório. Não foi observada diferença significativa quanto à área da ferida cutânea entre os grupos, apenas um efeito temporal na cicatrização (P>0,05), indicando estágio possivelmente mais avançado desse processo. Porém, na avaliação histológica, os grupos semente e controle apresentaram colagenização mais intensa que o grupo casca (P<0,05). Dipteryx alata Vogel mostrou-se inerte no processo de cicatrização de feridas em camundongos.

ABSTRACT The aim of this study was to evaluate the topical application of alcoholic extracts of Dipteryx alata Vogel almonds and bark in skin wound healing in mice. Fifty-four C57BL/6 mice were equally distributed into three groups: Control, Almond, and Bark. A 9 mm skin fragment was resected from the dorsal region of the animals’ thorax. The wounds were submitted to topical application of base cream (vehicle), 10% hydroalcoholic almond extract, or bark extract twice a day. Macroscopic, histological, and immunohistochemical evaluations were conducted on the 7th, 14th, and 21st postoperative days. No significant difference was observed regarding skin wound area among groups, with the parameter presenting only a temporal effect on healing (p>0.05). The almond and control groups exhibited more intense collagenization than the bark group (p<0.05). Dipteryx alata Vogel showed to be inert in the wound healing process in mice.

Resumo As características anatômicas da madeira são influenciadas pelas qualidades dos sítios de procedência do material, que por consequência leva a alterações em suas propriedades físicas e mecânicas. O objetivo deste trabalho foi avaliar e caracterizar as propriedades organolépticas e anatômicas da madeira de teca (Tectona grandis), proveniente de diferentes sítios do Estado do Mato Grosso, Brasil. Para as características anatômicas foram avaliados comprimento, largura, diâmetro e espessura da parede para fibra; comprimento e diâmetro dos vasos; comprimento, largura e frequência dos raios. Os resultados mostraram que as propriedades organolépticas não apresentaram diferenças entre os diferentes sítios. O efeito sítio, no qual estão estabelecidos os plantios de teca produziram variações significativas nos parâmetros das fibras (comprimento, largura da fibra, diâmetro do lume e espessura da parede) e nos raios (comprimento, largura e frequência). No entanto, não foram evidenciadas mudanças significativas relacionadas aos vasos.

Abstract The anatomical characteristics of the wood are influenced by the quality of the sites of the material provenance, which, consequently, leads to alterations in its physical and mechanical properties. The aim of this paper was to assess and characterize the organoleptic and anatomical properties of the teak (Tectona grandis) from different sites in the state of Mato Grosso, Brazil. Regarding the anatomical characteristics the length, width, diameter and the thickness of the wall for fiber were assessed; the length and diameter of the vessels; the length, width and frequency of the rays. The results obtained showed that the organoleptic properties do not present variations between the different sites. The effect of the site, where the teak plantation is established, produced significant variations in the parameters of the fibers (length, width of the fiber, lumen diameter and thickness of the wall) and in the rays (length, width and frequency). However, no significant vessel-related changes were seen.

ABSTRACT Wood color and properties are variables among and within species, and fast and non-destructive techniques can be applied to their characterization, being also important in wood marketing and quality control. This paper evaluated the influence of age on the discrimination of Tectona grandis L.F. (teak) wood by VIS/NIR spectroscopy. Wood from three ages, with heartwood and sapwood, were studied, totaling 36 samples per age. Quantitative colorimetric data, based on CIELAB 1976, visible and NIR infrared spectra were collected from radial and tangential surfaces in five positions of each sample, in a total of 540 spectra. Both techniques were adequate for age discrimination in teak wood. Statistical differences were observed in the chromatic coordinates in heartwood and sapwood for each age. VIS/NIR spectroscopy can be applied for age discrimination based on teak solid samples and for wood quality control.

RESUMO Compostos químicos com atividades biológicas isolados de espécies vegetais e usados pela população por meio de extratos e óleo essencial são promissoras fontes de prospecção de novos fármacos. O gênero Myroxylon L. f. apresenta elevado potencial farmacológico, sendo usado na medicina popular para o tratamento de diversas enfermidades, tais como feridas, catarros, úlceras, dores de cabeça e de ouvido. Este artigo de revisão apresenta as principais características fitoquímicas e farmacológicas das espécies do gênero Myroxylon (M. peruiferum L.f., M. balsamum (L.) Harms e M. pereirae (Royle) Klotzsch), cujos estudos de atividades biológicas são promissores.

ABSTRACT Chemical compounds with biological activities isolated from plant and used by the population through extracts and essential oils are promising sources of prospection for new drugs. The genus Myroxylon L. f. showed high pharmacological potential being used in popular medicine for the treatment of various diseases such as wounds, colds, ulcers, headache, and ear infections. This review article presents the main phytochemical and pharmacological characteristics of the species of Myroxylon (M. peruiferum L.f., M. balsamum (L.) Harms and M. pereirae (Royle) Klotzsch), whose studies of biological activities are promising.

Abstract Researches of environmental perception evidences the extractive context and help policy making. Despite the social and economic benefits, the intensification of extractivism can promote ecological unsustainability and alteration in the perception of forest conservation values. In the region of Lençóis Maranhenses, Northeast Brazil, the sale of products extracted from Mauritia flexuosa L.f palm to supply the local and national market is increasing. Thus, we aimed to evaluate the forest values perceived by extractivists in municipalities of the region of Lençóis Maranhenses and how the socioeconomic profile interfere with the environmental perceptions of the interviewees. The research was conducted through semi-structured forms applied to 271 extractivists from the municipality of Barreirinhas, Paulino Neves and Tutóia. The perceived forest values were divided into three categories: economic, ecological and social. It was contacted that there was a prioritization of the values included in the Economic category, in which the source of income is highlighted in the municipalities that most extract the palms. The data revealed that there was a prioritization of the economic category, with emphasis on the forest value source of income. In Barreirinhas, food and climate regulation values were related to socioeconomic characteristics such as gender and monthly income from the sale of handicrafts. There is knowledge about the environmental damages that occur in the forest. However, there is no effective participation of the communities in the management of the Environmental Conservation Units of the region.

Resumo Pesquisas de percepção ambiental evidenciam o contexto extrativista e auxiliam na elaboração de políticas ambientais. Apesar dos benefícios sociais e econômicos, a intensificação do extrativismo pode promover a insustentabilidade ecológica e alteração da percepção dos valores florestais conservacionistas. Na região dos Lençóis Maranhenses, Nordeste do Brasil, é crescente a venda de produtos extraídos da palmeira Mauritia flexuosa L.f. para abastecer mercado local e nacional. Desta forma, o presente trabalho objetivou avaliar quais valores florestais são percebidos pelos extrativistas em municípios da região dos Lençóis Maranhenses e como o perfil socioeconômico interfere na percepção ambiental dos entrevistados. A pesquisa foi conduzida por meio de formulários semiestruturados aplicados a 271 extrativistas dos município de Barreirinhas, Paulino Neves e Tutóia. Os valores florestais percebidos foram divididos em três categorias: econômica, ecológica e social. Os dados obtidos revelaram que houve uma priorização da categoria econômica, com destaque para o valor florestal fonte de renda. Em Barreirinhas, os valores alimentação e regulação do clima estiveram relacionados a características socioeconômicas tais como gênero e renda mensal proveniente da venda do artesanato. Há o conhecimento sobre os danos ambientais que ocorrem nos buritizais, entretanto não existe uma efetiva participação das comunidades na gestão das Unidades de Conservação ambientais da região.

RESUMO A energia é considerada o nutriente mais limitante para produção de leite de vacas em pastagens, mas será que a suplementação deve ser energética? Assim, objetivou-se avaliar a resposta produtiva, marginal e econômica de vacas Girolandas mantidas em pastagem de Tifton 85 durante o período das águas, as quais receberam diferentes tipos de suplementação. O primeiro tratamento foi a suplementação mineral ad libitum; o segundo tratamento, o fornecimento de 1,0kg/dia de suplementação proteica; o terceiro e quarto tratamentos, o fornecimento de 2,0kg/dia de suplementação proteico-energética e 4,0kg/dia de suplementação energética, respectivamente. Foram utilizadas 12 vacas com produção de leite média de 15,0±1,99kg/dia. Os dados foram avaliados como três quadrados latinos 4 x 4 simultâneos ao nível de 5% de significância. O consumo de matéria seca de forragem não foi influenciado pelas suplementações (P>0,05). A maior produção de leite (P<0,05) foi observada com a suplementação energética, em média 14,73kg/dia, não diferente da produção com a suplementação proteico-energética, média de 13,84kg de leite/dia. Observaram-se respostas produtivas marginais de 1,03; 0,84 e 0,44kg de leite/kg de concentrado, para suplemento proteico, proteico-energético e energético, respectivamente, bem como saldo financeiro positivo para as suplementações proteica e proteico-energética em relação à suplementação mineral. A suplementação proteico-energética mostrou-se a mais eficiente.

ABSTRACT Energy is considered the most limiting nutrient for the production of milk cows in pastures, but will that supplementation must be energy? The objective was to evaluate the productive, marginal and economic response of Girolando cows kept in Tifton 85 pasture during rain period receiving different types of supplementation. One treatment was mineral supplementation ad libitum, a second treatment providing 1.0kg/day of protein supplementation, and a third and fourth treatments providing 2.0kg/day of protein-energetic supplementation and 4.0kg/day of energy supplementation, respectively. Twelve cows were used with average milk production 15.0±1.99kg/day. The data were evaluated as three Latin squares 4 x 4 simultaneous to the 5% level of significance. The dry matter intake of forage not influenced by supplementation (P> 0.05). The higher milk production was observed with energetic supplementation on average 14.73kg of milk/day, no different of production obtained from protein-energy supplementation, average of 13.84kg of milk/day. Marginal productive response was observed, with responses of 1.03; 0.84 and 0.44kg milk/kg concentrate for proteic supplement, proteic-energetic, and energetic, respectively. Positive financial balance was found for protein and protein-energy supplements in relation to mineral supplementation. Protein-energy supplementation proved the most efficient.