Abstract An analytical method applying ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was validated for the determination of aflatoxins M2, M1, G2, G1, B2, B1, deoxynivalenol, ochratoxin A, fumonisins B1 and B2, hydrolyzed fumonisins B1 and B2, zearalenone and sterigmatocystin in crackers. The obtained recoveries (70 to 110%) and relative standard deviations (< 13%) were considered satisfactory. Method limits of quantification ranged from 0.20 µg kg-1 (sterigmatocystin) to 12.27 µg kg-1 (deoxynivalenol). The validated method was then applied for mycotoxin determination in 60 cracker samples (cream crackers and water and salt crackers) obtained from the metropolitan area of the state of Rio de Janeiro, RJ. Deoxynivalenol, zearalenone and fumonisin B1 were found, respectively, in 100, 50 and 28% of the analyzed samples. The maximum permissible limits established by Brazilian legislation for deoxynivalenol and zearalenone were not exceeded in the analyzed samples.

ABSTRACT PURPOSE : To compare ileal anastomoses in the immediate postoperative healing period after meloxicam use. METHODS: Forty two male Wistar rats were randomly divided into two groups of 21, COX and control group. To COX meloxicam in combination with morphine was given in 3 days period. Control group received only morphine during the same period. Each group was divided into three sub-groups of 7, which were euthanized at 5, 10, and 21 days postoperatively. Comparison was based in histological evaluation of collagen type I and III using sirius red, immunohistochemical through vascular endothelial growth factor and matrix metalloproteinase-9. RESULTS: Healing process in scheduled periods did not show significant differences (p>0.05) between the COX and control groups during any of the periods. CONCLUSION: The use of meloxicam in the postoperative period following ileal anastomosis did not affect healing.

O objetivo deste estudo in vitro foi avaliar a resistência à tração de pinos de fibra de vidro submetidos a diferentes tratamentos de superfície. Quarenta e oito caninos superiores tiveram suas coroas seccionadas e as raízes tratadas endodonticamente. As raízes foram incluídas em resina acrílica e distribuídas em 3 grupos de acordo com o tratamento de superfície aplicado aos pinos: Grupo I: os pinos receberam agente silano por 30 s e adesivo; Grupo II: os pinos foram limpos com álcool e então receberam o agente silano e adesivo; Grupo III: os pinos foram submetidos ao condicionamento com ácido fosfórico a 37% por 30 s, seguido de agente silano e adesivo. Cada grupo foi dividido em 2 subgrupos de acordo com fotoativação do adesivo antes da inserção do pino no interior do canal: A – adesivo não foi fotoativado, B: o adesivo foi fotoativado. Todos os pinos foram cimentados com Panavia F e as amostras foram submetidas a teste de tração utilizando máquina universal de ensaios, com velocidade de 1 mm/min. Os dados obtidos foram submetidos à ANOVA a um critério e ao teste de Tukey com nível de significância de 5%. Houve diferença estatisticamente significante (p<0,01) entre o grupo GIII-B e os grupos GI-A e GI-B. Os demais grupos não apresentaram diferenças significantes entre si (p>0,05). Pode-se concluir que as substâncias utilizadas para a limpeza dos pinos interferiram em sua retenção, independente da fotopolimerização do adesivo.

The aim of this in vitro study was to evaluate the tensile strength of glass fiber posts submitted to different surface treatments. Forty-eight maxillary canines had their crowns sectioned and root canals endodontically treated. The roots were embedded in acrylic resin and distributed into 3 groups according to the surface treatment: Group I: the posts were treated with silane agent for 30 s and adhesive; Group II: the posts were cleaned with alcohol before treatment with silane agent and adhesive; Group III: the posts were submitted to conditioning with 37% phosphoric acid for 30 s before treatment with silane agent and adhesive. Each group was divided into 2 subgroups for adhesive polymerization or not before insertion into the canal: A - adhesive was not light cured and B - adhesive was light cured. All posts were cemented with Panavia F and the samples were subjected to tensile strength test in a universal testing machine at crosshead speed of 1 mm/min. Data were submitted to one-way ANOVA and Tukey's test at 5% significance level. There was statistically significant difference (p<0.01) only between group GIII-B and groups GI-A and GI-B. No significant difference was found among the other groups (p>0.05). It was concluded that the products used for cleaning the posts influenced the retention regardless of adhesive light curing.

The aim of this study was to evaluate the influence of different restorative procedures on the fracture resistance of endodontically treated teeth submitted to intracoronal bleaching. Fifty upper central incisors were distributed into 5 groups: GI - healthy teeth; GII - endodontically treated teeth sealed with Coltosol; GIII - endodontically treated teeth bleached and sealed with Coltosol; GIV - endodontically treated teeth bleached and restored with composite resin; and GV - endodontically treated teeth bleached and restored with a fiberglass post and composite resin. In the bleached specimens, a cervical seal was made prior to bleaching with 38% hydrogen peroxide. The gel was applied on the buccal surface and in the pulp chamber, and was then light-activated for 45 s. This procedure was repeated three times per session for four sessions, and each group was submitted to the restorative procedures described above. The specimens were submitted to fracture resistance testing in a universal testing machine. There were statistically significant differences among the groups (p < 0.05). The mean value found for GIII was the lowest (0.32 kN) and was significantly different from the values found for GI (0.75 kN), GII (0.67 kN), GIV (0.70 kN), and GV (0.72 kN), which were not significantly different from each other (p > 0.05). The restorative procedures using composite resin were found to successfully restore the fracture resistance of endodontically treated and bleached teeth.

OBJETIVO: Avaliou-se in vitro a resistência à fratura de raízes com pinos metálicos fundidos e de fibra de vidro, variando sua configuração geométrica. METODOLOGIA: Cinquenta incisivos centrais superiores tratados endodonticamente foram incluídos em resina acrílica. Dez receberam apenas preparo coronário (controle) e quarenta tiveram coroas seccionadas e raízes incluídas em resina (deixando 4 mm cervicais). Distribuiu-se os espécimes em 5 grupos: controle, pino metálico cilíndrico, metálico cônico, pino de fibra de vidro cilíndrico e cônico. Submeteram-se os corpos-de-prova a ensaio de compressão, até ocorrer a fratura. RESULTADOS: A ANOVA indicou diferença significante entre os grupos (P<.05) e no teste de Tukey-Kramer´s o controle (867±243 N) apresentou os maiores valores de resistência à fratura, sendo similar ao grupo do pino de fibra cilíndrico (711±180 N). Não houve diferença significante entre os pinos metálicos cilíndricos (435±245 N) ou cônicos (585±164 N) e os de fibra cônicos (453±112 N). Os grupos dos pinos de fibra cilíndricos (711±180 N) e pinos metálicos cônicos (585±164 N) apresentaram valores intermediários e não foram diferentes entre si. CONCLUSÃO: Pinos de fibra de vidro são uma alternativa viável ao pino metálico fundido cilíndrico e cônico. Os pinos de fibra cilíndricos aumentaram a resistência à fratura dos incisivos tratados endodonticamente.

PURPOSE: To evaluate the in vitro fracture resistance of roots with glass-fiber and metal dowels with different designs. METHODS: Fifty-endodontically treated maxillary central incisors were embedded in acrylic resin. Ten of them received only the coronary preparation, and the remaining forty were embedded (except for 4mm of the cervical area) after removing the clinical crowns. Specimens were divided into five groups (n=10): control (teeth with only coronary preparation), cylindrical cast dowel, conical cast dowel, cylindrical glass-fiber dowel and conical glass-fiber dowel. Specimens were subjected to an increasing compressive load (N) until fracture. RESULTS: ANOVA indicated significant difference (P<.05) among the groups, and the Tukey-Kramer´s test identified these differences. The control group (867±243 N) presented the highest values and was statistically similar to cylindrical glass-fiber dowel group (711±180 N). There is no significant difference among the metal dowel cylindrical (435±245 N) or conical (585±164 N) group and conical glass-fiber dowel (453±112 N). Cylindrical glass-fiber dowel (711±180 N) and conical cast dowel and core (585±164 N) groups had intermediate values and did not differ from each other. CONCLUSIONS: Cylindrical glass fiber dowels represent a viable alternative to the cast-metal dowel cylindrical or conical. Cylindrical glass fiber dowels also increase endodontically treated incisors' resistance to fracture.

OBJECTIVE: This study evaluated in vitro the influence of an eugenol-based sealer (EndoFill) on the retention of stainless steel prefabricated posts cemented with zinc phosphate and resin-based (Panavia F) cements after different periods of root canal obturation, using the pull-out test. MATERIAL AND METHODS: Sixty upper canines were decoronated and the roots were embedded in resin blocks. The specimens were distributed into 3 groups, according to the period elapsed between canal obturation and post cementation: Group I - immediately; Group II - 72 h and Group III - 4 months. The groups were subdivided according to the type of cement used for post cementation: A - zinc phosphate and B - Panavia F. Following the experimental periods, specimens were subjected to pullout test in an Instron machine with application of tensile force at a crosshead speed of 0.5 mm/min until post dislodgement. The maximum forces required for post removal were recorded (kN) and means were subjected to statistical analysis by 2-way ANOVA and Tukey-Kramer test (α=0.001) RESULTS: There were statistically significant differences (p<0.01) between the posts cemented with zinc phosphate cement (0.2112 kN) and Panavia F (0.0501 kN). However, no statistically significant differences (p>0.05) were found between the three post cementation periods, regardless of the cement. CONCLUSIONS: It was concluded that the eugenol-based sealer influenced the tensile strength of the posts cemented with the resin cement, but had no influence on the time waited between root canal obturation and post space preparation/post cementation.

O objetivo deste trabalho foi avaliar o efeito do uso de espaçadores na adaptação marginal de "copings" de ligas de NiCr (M1) e NiCrBe (M2) e titânio (M3) fundidos pela técnica de cera perdida. A partir de uma matriz metálica, foram obtidos 45 troquéis de gesso resinado tipo IV para confecção dos padrões de cera nas seguintes condições: sem a presença de espaçador (A), com uma camada de espaçador (B) e com duas camadas de espaçador (C), com cinco repetições para cada condição (liga x espaçador). Foi realizado o enceramento de cada troquel e os padrões de cera foram incluídos no revestimento indicado pelo fabricante. Em cada anel de fundição foram incluídos 3 padrões de cera, sendo cada um correspondente a cada condição. Cada "coping", adaptado à matriz metálica por meio de um dispositivo padronizador da pressão de assentamento, foi levado ao microscópio óptico para aferição das medidas da desadaptação marginal. Os dados obtidos (µm) foram submetidos à análise estatística por ANOVA e teste de Tukey (a=5%). Entre os materiais houve diferença estatisticamente significante (M1=110,67, M2=130,33 e M3=148,33). Em relação ao fator espaçador, houve diferença estatisticamente significante entre as três condições (A=162,00; B=131,06 e C=96,67). Conclui-se que a presença de duas camadas de espaçador propicia menor desadaptação marginal.

The goal of this study was to evaluate the effect of using die spacers on the marginal fit of NiCr (M1) and NiCrBe (M2) alloys and commercially pure titanium (cpTi) (M3) copings cast by the lost wax technique. Using a metal matrix, 45 resin added extra hard type IV stone models were obtained for the fabrication of wax patterns under the following conditions: no die spacer (A), with one die spacer layer (B) and with two die spacer layers (C), with five repetitions for each condition (alloy x die). Each die was waxed and the wax patterns were invested as per manufacturer's instructions. Three wax patterns were embedded in each casting ring, each corresponding to one of the conditions. Each coping, seated to the metal matrix by a seating pressure standardizing device (SPSD), was taken to an optical microscope for measurement of marginal discrepancy. The obtained data (µm) were analyzed statistically by ANOVA and Tukey' test (a=5%). There was statistically significant difference (p<0.05) among the materials (M1=110.67; M2=130.33 and M3=148.33). Regarding the use of the die spacer, there was a statistically significant difference (p<0.05) among the three conditions (A=162.00; B=131.06 and C=96.67). It was concluded that there is less marginal discrepancy with two die spacer layers.

No presente estudo avaliou-se, in vitro, a influência do cimento endodôntico à base de óxido de zinco e eugenol (EndoFill) na adesão de pinos intra-radiculares cimentados com cimento resinoso (Enforce) ou cimento fosfato de zinco. Vinte e quatro caninos superiores uniradiculares foram distribuídos em dois grupos e obturados com cimento a base de oxido de zinco e eugenol + cones de guta-percha ou somente cones de guta-percha (sem cimento). Em metade dos espécimes em cada grupo (n=6), os pinos intra-radiculares foram cimentados com cimento resinoso Enforce e na outra metade com cimento de fosfato de zinco. Os espécimes foram submetidos a ensaios de resistência à tração numa máquina Instron 4444, sendo os valores de força máxima necessária ao desprendimento dos retentores registrados e submetidos à análise estatística pelo teste de Kruskal-Wallis (p<0,01). Os pinos cimentos com cimento fosfato de zinco apresentaram valor médio de resistência à tração superior (353,4 N) ao dos pinos cimentados com Enforce (134,9 N). Em relação à influência do cimento à base de eugenol na retenção dos pinos intra-radiculares, houve diferença significante (p<0,01) somente entre os grupos cimentados com Enforce, sendo que nos canais obturados com EndoFill + guta-percha houve menor resistência à tração que nos canais obturados apenas com guta-percha (respectivamente 101,5 e 168,2 N). Conclui-se que o cimento à base de fosfato de zinco apresentou maior retenção que o cimento resinoso Enforce e que o cimento obturador contendo eugenol (EndoFill) somente afetou as propriedades adesivas do cimento resinoso.

The present study evaluated, in vitro, the influence of an eugenol-based endodontic sealer (EndoFill) on the adhesion of intra-radicular posts cemented with a resin-based cement (Enforce) ou a zinc phosphate cement. Twenty-four single-rooted maxillary canines were divided into 2 groups (n=12) and obturated with either gutta-percha points plus EndoFill or gutta-percha points alone (no cement). In each group, half of intracanal posts (n=6) were cemented with Enforce resin-based cement and half with zinc phosphate cement. Specimens were submitted to pull-out test in an Instron machine and tensile force was applied at a crosshead speed of 0.5 mm/min until post dislodgement. The maximum forces required for post removal was recorded (N) and means were submitted to statistical analysis by Kruskal-Wallis test (p<0.01). Posts cemented with zinc phosphate cement were significantly more retentive (353.4 N) than those cemented with Enforce (134.9 N) (p<0.01). Regarding the influence of the eugenol-based cement (EndoFill) on post retention, there was statistically significant difference (p<0.01) only between the groups cemented with Enforce, i.e., in the canals filled with EndoFill + guta-percha there was lower bond strength than in the canals filled with gutta-percha points alone (101.5 and 168.2 N, respectively). In conclusion, the zinc-phosphate-based cement showed greater post retention than the resin-based cement. The findings of this study suggest that the eugenol-containing sealer interfered with the adhesive properties of the resin-based cement.

Este estudo in vitro comparou a resistência à remoção por tração de núcleos metálicos fundidos em liga de Cu-Al (Goldent-LA) cilíndricos, jateados, de comprimento constante igual a 9 mm, cimentados com cimento de fosfato de zinco e com três diferentes diâmetros: 0,9 mm, 1,3 mm e 1,7 mm. Trinta e seis caninos superiores hígidos tiveram suas coroas seccionadas, sendo as raízes incluídas em blocos de resina acrílica, e os canais, tratados endodonticamente. Os dentes foram divididos em três grupos para serem preparados e padronizados com o auxílio de um paralelômetro com as seguintes brocas: Grupo 1 - Largo nº 2; Grupo 2 - Largo nº 4; Grupo 3 - Largo nº 6. Os núcleos foram moldados com resina acrílica ativada quimicamente e, após a fundição, foram jateados e tiveram as suas dimensões conferidas com um paquímetro digital. Após a cimentação, os corpos-de-prova foram armazenados em água destilada durante 7 dias, em estufa a 37ºC e, posteriormente, submetidos a teste de tração em uma máquina universal de ensaios Instron 4444. Com a análise estatística dos resultados, pôde-se concluir que não houve diferença estatisticamente significante entre os grupos testados. A variação do diâmetro em núcleos cilíndricos jateados cimentados com fosfato de zinco não afetou a resistência à remoção.

This study compared the resistance to removal by traction of abraded cylindrical metal cast posts of Cu-Al (Goldent-LA). The posts had constant length (9 mm) and three different diameters (0.9, 1.3 and 1.7 mm), and were cemented with zinc phosphate cement. The crowns of 36 sound maxillary canines were sectioned, the roots were immersed in resin blocks and the root canals were endodontically treated. The teeth were divided into three groups to be prepared and standardized with the use of a parallelometer with the following burs: Group 1 - Largo n. 2; Group 2 - Largo n. 4; Group 3 - Largo n. 6. The posts were molded with chemically activated resin and after casting they were abraded and their dimensions were confirmed with a digital caliper. After cementation of the posts in the prepared root canals, the samples were kept at 37ºC in distilled water for 7 days and subsequently submitted to the traction test in a universal testing machine (Instron 4444). The results showed no statistical difference between the groups. Diameter variation (0.9 mm, 1.3 mm and 1.7 mm) in abraded cylindrical posts cemented with zinc phosphate did not affect resistance to removal.