Abstract This study aimed to analyze the phytochemical profile of essential oil obtained from the leaves of Coriandrum sativum L., and its antifungal activity against Candida spp. The research consisted of an in vitro study including collecting the vegetable product, analysis of its macronutrients, extraction, and chemical analysis of the essential oil, and assaying antifungal activity through minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), with growth inhibition kinetics, and the product’s effects on multi-species Candida biofilm. Nitrogen (47.08 g Kg-1), phosphorus (5.3 g Kg-1) and potassium (50.46 g Kg-1) levels were within the normal range. The major constituents were octanal, decanal, dec-(2E)-enal, and dodecanal. The MIC and MFC of the product evaluated against 11 tested Candida strains ranged from 31.25 to 250 μg/mL. There was inhibition of fungal growth during 24 hours of exposure at the 3 concentrations tested (250, 125, and 62.5 μg/mL). The concentration of 80 mg/mL promoted the greatest reduction in multispecies biofilm (70% reduction in biofilm). Coriandrum sativum L. essential oil extract is principally constituted of alcohols and aldehydes and presents fungicidal activity against Candida spp. in its in planktonic and biofilm forms.

Resumo Este trabalho teve como objetivo analisar o perfil fitoquímico do óleo essencial obtido das folhas de Coriandrum sativum L., e sua atividade antifúngica contra Candida spp. A pesquisa consistiu em um estudo in vitro incluindo a coleta do produto vegetal, análise de seus macronutrientes, extração e análise química do óleo essencial e ensaio da atividade antifúngica por meio da concentração inibitória mínima (CIM) e concentração fungicida mínima (MFC), com crescimento cinética de inibição e os efeitos do produto no biofilme de Candida multi-espécies. Os níveis de nitrogênio (47,08 g Kg-1), fósforo (5,3 g Kg-1) e potássio (50,46 g Kg-1) estavam dentro da normalidade. Os principais constituintes foram octanal, decanal, dec-(2E)-enal e dodecanal. A CIM e CFM do produto avaliado contra 11 cepas de Candida testadas variaram de 31,25 a 250 μg/mL. Houve inibição do crescimento fúngico durante 24 horas de exposição nas 3 concentrações testadas (250, 125 e 62,5 μg/mL). A concentração de 80 mg/mL promoveu a maior redução no biofilme multiespécies (redução de 70% no biofilme). O extrato do óleo essencial de Coriandrum sativum L. é constituído principalmente por álcoois e aldeídos e apresenta atividade fungicida contra Candida spp. em suas formas planctônicas e biofilme.

Abstract This study compares the morphology, thermal, and dynamic-mechanical properties of composites based on polybutylene adipate terephthalate/polylactide biocomposites with sponge gourd waste treated code as R, and non-treated sponge gourd, coded as NR, by mechanical disc refining after milled process. Extrusion followed by compression molding was used to produce biocomposites with fiber contents of 0, 2.5, 5, 10, and 15% wt/wt for R and NR sponge gourd fibers. Scanning electron microscopy analysis reveals that NR has the morphology of a rigid tubular shape, whereas R is a thinner, twisted, and fibrillated fiber. Regardless of the type of sponge gourd fiber used, the thermal stability of the composite decreases as the sponge gourd content increases. At 25°C, the biocomposite with 10%wt/wt R fiber has the highest storage modulus value. The comparison of Tangent peak values reveals that the presence of sponge gourd fibers reduces the energy dissipation of the biocomposites. The analysis of the loss modulus at 25°C reveals that R fiber contributes more to the reduction of energy dissipation of the biocomposites than NR. Furthermore, the Cole-Cole plot shows that R and NR fibers are dispersed and do not significantly change the homogeneity of the biopolymer systems.

Abstract Gliomas represent 80% of all primary malignant brain tumors in adults. In view of this public health problem, the early detection through sensitive and specific molecular tumor markers analysis can help to improve gliomas diagnosis and prognosis as well as their staging, assessment of therapeutic response and detection of recurrence. Therefore, this review focuses in current gliomas tumor markers, IDH-1/2, 1p/19q, MGMT, ATRX, TERT, H3, EGFR, BRAF and Ki67 used in clinic worldwide and their importance to early detection, glioma histological and molecular classification as well as in predicting patient’s therapeutic response. In addition, we present what are the steps in the requesting process for this type of examination in the Brazilian Public Health System (SUS) scope, which attends most of the Brazilian population. Thereby, this article is useful in demonstrating which markers are used in the clinical practice for glioma patients and can be performed in the SUS through partnerships/agreements between specialized health centers and clinical analysis laboratories. It is hoped that this work clarifies, the necessary subsidies to carry out the research of tumor markers in all institutions that serve SUS users, providing a service with equal conditions.

ABSTRACT Objectives: To evaluate the impact of COVID-19 on clinical and surgical practice, educational activities, health and lifestyle behavior of Brazilian urology residents. Materials and Methods: A web-based survey was sent to 468 Brazilian urology residents from postgraduate years (PGY) 3 to 5 to collect data on clinical practice and training after 4 months of COVID-19. We also assessed health-related and behavior changes, rate of infection by SARS-CoV-2, deployment to the front line of COVID-19, residents’ concerns, and access to personal protective equipment (PPE). Results: Massive reductions in elective and emergency patient consultations, diagnostic procedures and surgeries were reported across the country, affecting PGY 3 to 5 alike. Most in-person educational activities were abolished. The median damage to the urological training expected for 2020 was 6.0 [3.4 – 7.7], on a scale from 0 to 10, with senior residents estimating a greater damage (P< 0.001). Educational interventions developed included online case-based discussions, subspeciality conferences and lectures, and grand rounds. Most senior residents favored extending residency to compensate for training loss and most younger residents favored no additional training (p< 0.001). Modifications in health and lifestyle included weight gain (43.8%), reduced physical activity (68.6%), increased alcoholic intake (44.9%) and cigarette consumption (53.6%), worsening of sexual life (25.2%) and feelings of sadness or depression (48,2%). Almost half were summoned to work on the COVID-19 front-line and 24.4% had COVID-19. Most residents had inadequate training to deal with COVID-19 patients and most reported a shortage of PPE. Residents’ concerns included the risk of contaminating family members, being away from residency program, developing severe COVID-19 and overloading colleagues. Conclusions: COVID-19 had a massive impact in Brazilian urology residents´ training, health and lifestyle behavior, which may reflect what happened in other medical specialties. Studies should confirm these findings to help developing strategies to mitigate residents’ losses.

SUMMARY Acromegaly caused by ectopic growth hormone-releasing hormone (GHRH)-secreting tumor is exceedingly rare. We report a case of acromegaly secondary to GHRH secretion by an incidentally diagnosed pulmonary neuroendocrine tumor (NET) and review 47 similar cases in literature. A 22-year-old male patient presented with symptoms of pituitary apoplexy. Magnetic resonance imaging (MRI) showed apoplexy of a pituitary adenoma. Routinely prior to surgery, a chest radiography was performed which revealed a mass in the left lung. During investigation, the patient was diagnosed with metastatic GHRH-secreting pulmonary NET. In retrospect, it was noted that the patient had pituitary hyperplasia 20 months prior to the MRI which showed the presence of a pituitary adenoma. The histological findings confirmed somatotroph hyperplasia adjacent to somatotropinoma. This case suggests that GHRH secretion can be associated with pituitary hyperplasia, which may be followed by pituitary adenoma formation.

Abstract A multiple primary malignancy (MPN) is evidence of two or more histologically different malignancies presenting in the same patient. A series of cases have been reported worldwide, however, in Venezuela there are no cases or reported incidence. The following is the case of an 82-year-old patient in whom the coexistence of a renal sarcomatoid carcinoma with chronic lymphocytic leukemia, the association of which is poorly described. Early diagnosis of MPN leads to effective treatment, giving the patient better survival and quality of life.

Resumen Una neoplasia maligna primaria múltiple (NMPM) es la evidencia de dos o más tumores malignos histológicamente diferentes presentados en el mismo paciente. Mundialmente se han reportado una serie de casos, sin embargo, en Venezuela no existen casos ni incidencia reportada. A continuación, se presenta el caso de una paciente de 82 años en quien se demostró la coexistencia de un carcinoma sarcomatoide renal con leucemia linfocítica crónica, cuya asociación es poco descrita. El diagnóstico precoz de las NMPM conduce a un tratamiento eficaz, confiriendo al paciente mejor supervivencia y calidad de vida.

ABSTRACT Objectives To evaluate the impact of COVID-19 on clinical practice, income, health and lifestyle behavior of Brazilian urologists during the month of April 2020. Materials and Methods A 39-question, web-based survey was sent to all urologist members of the Brazilian Society of Urology. We assessed socio-demographic, professional, health and behavior parameters. The primary goal was to evaluate changes in urologists’ clinical practice and income after two months of COVID-19. We also looked at geographical differences based on the incidence rates of COVID-19 in different states. Results Among 766 urologists who completed the survey, a reduction ≥ 50% of patient visits, elective and emergency surgeries was reported by 83.2%, 89.6% and 54.8%, respectively. An income reduction of ≥ 50% was reported by 54.3%. Measures to reduce costs were implemented by most. Video consultations were performed by 38.7%. Modifications in health and lifestyle included weight gain (32.9%), reduced physical activity (60.0%), increased alcoholic intake (39.9%) and reduced sexual activity (34.9%). Finally, 13.5% of Brazilian urologists were infected with SARS-CoV-2 and about one third required hospitalization. Urologists from the highest COVID-19 incidence states were at a higher risk to have a reduction of patient visits and non-essential surgeries (OR=2.95, 95% CI 1.86 – 4.75; p< 0.0001) and of being infected with SARS-CoV-2 (OR=4.36 95%CI 1.74-10.54, p=0.012). Conclusions COVID-19 produced massive disturbances in Brazilian urologists’ practice, with major reductions in patient visits and surgical procedures. Distressing consequences were also observed on physicians’ income, health and personal lives. These findings are probably applicable to other medical specialties.

The use of sorghum in human nutrition has been expanded due to its nutritional composition and its functional potential. Extrusion has been used to improve the quality of sorghum. The aim of this work was to assess the effect of extrusion in the nutritional composition, phenolic compounds content of two sorghum, and characterize the profile of chemical constituents by paper spray mass spectrometry (PS-MS). Extrusion increased the carbohydrate and fiber contents and reduced moisture, lipids and ashes, and enabling a higher degree of liberation of phenolic compounds and antioxidant activity. The fingerprint obtained in both ionization modes had been influenced by extrusion, allowing for a greater compounds’ identification on the extruded samples. The analysis of sorghum samples’ main components made distinguishing of the main constituents possible, as well as highlighted the extrusion effect and the influence of the genotypes. This study demonstrated that extrusion enabling a higher liberation of phenolic compounds, which were identified by the PS-MS technique.

ABSTRACT The lemon (Citrus aurantifolia) crop is susceptible to attack by phytopathogens, which are controlled with chemical molecules. An alternative to reverse the ecological damage caused by these chemicals is biological control. The aim of this study was to select strains of actinomycetes with antagonistic activity to Colletotrichum gloeosporioides. It was determined the antagonistic activity in vitro of 181 strains of actinomycetes, belonging to the Chemical Bioactive Center's strain, against C. gloeosporioides; as well as, the extracellular enzymatic activity of the strains that showed better antagonistic effect. From the total of evaluated strains, 62 showed antagonistic capacity manifested against C. gloeosporioides, indicating that they have the potential to be evaluated in vitro and in vivo against different lemon phytopathogens.

RESUMEN El cultivo de limón (Citrus aurantifolia) es susceptible al ataque de fitopatógenos, que son controlados con moléculas químicas. Una alternativa para revertir los daños ecológicos causados por estas sustancias químicas es el control biológico. El objetivo del presente estudio fue seleccionar cepas de actinomicetos con actividad antagónica a Colletotrichum gloeosporioides. Se determinó la actividad antagónica in vitro de 181 cepas de actinomicetos, pertenecientes al cepario del Centro de Bioactivos Químicos, contra C. gloeosporioides; así como, la actividad enzimática extracelular de las cepas que exhibieron mejor efecto antagonista. Del total de cepas evaluadas, 62 mostraron capacidad antagónica manifestada contra C. gloeosporioides, indica que tienen el potencial para ser evaluadas in vitro e in vivo contra diferentes fitopatógenos del limón.

RESUMO Objetivo: o presente estudo avaliou os efeitos dentoesqueléticos e tegumentares em pacientes com má oclusão de Classe II tratados com aparelho Distal Jet, comparando-os com um grupo controle não tratado. Métodos: 44 pacientes com má oclusão de Classe II foram divididos em dois grupos: Grupo 1 (experimental) - 22 pacientes, idade média de 12,7 anos, tratados com o aparelho Distal Jet por um período médio de 1,2 anos; Grupo 2 (controle) - 22 pacientes não tratados, idade média de 12,2 anos, acompanhados por um período médio de 1,2 anos. Telerradiografias laterais foram obtidas antes do tratamento (T0) e no final da distalização (T1). O teste t independente foi usado para identificar as diferenças entre os grupos. Resultados: quando comparado ao grupo controle, o Distal Jet produziu um aumento significativo no ângulo do plano mandibular (0,7 ± 2,0o). Os segundos molares superiores apresentaram inclinação distal (6,6 ± 3,8o), distalização (1,1 ± 1,1 mm) e extrusão (1,3 ± 2,1 mm). Os primeiros molares superiores foram distalizados por 1,2 ± 1,4 mm. Os primeiros pré-molares superiores, mesializados por 3,4 ± 1,1 mm. Os incisivos superiores mostraram leve inclinação labial de 4,3 ± 4,7o e foram protruídos por 2,4 ± 1,7 mm. Não existiram alterações significativas no perfil facial. O overjet aumentou 1,5 ± 1,1 mm, e o overbite não sofreu alterações significativas. Conclusão: o aparelho Distal Jet é eficaz para distalizar os primeiros molares superiores, mas promove aumento no ângulo do plano mandibular, inclinação distal, extrusão e distalização dos segundos molares superiores, mesialização dos primeiros pré-molares superiores, vestibularização e protrusão dos incisivos superiores e aumento do overjet, quando comparado a um grupo de controle.

ABSTRACT Objective: This study evaluated the dental, skeletal and soft tissue effects in Class II malocclusion patients treated with Distal Jet appliance, compared to an untreated control group. Methods: 44 patients with Class II malocclusion were divided into two groups: Group 1 (experimental) - 22 patients, mean age of 12.7 years, treated with the Distal Jet appliance for a mean period of 1.2 years; Group 2 (control) - 22 untreated patients, mean age of 12.2 years, followed by a mean period of 1.2 years. Lateral cephalograms were obtained before treatment (T0) and at the end of the distalization (T1).Independent t test was used to identify intergroup differences. Results: When compared to control group, the Distal Jet produced a significant increase in mandibular plane angle (0.7 ± 2.0o). The maxillary second molars presented distal inclination (6.6 ± 3.8o), distalization (1.1 ± 1.1 mm) and extrusion (1.3 ± 2.1 mm). The maxillary first molars distalized by 1.2 ± 1.4 mm. The maxillary first premolars mesialized by 3.4 ± 1.1 mm. The maxillary incisors showed slight labial tipping of 4.3 ± 4.7o and were protruded by 2.4 ± 1.7 mm. There were no significant changes in the facial profile. The overjet increased 1.5 ± 1.1 mm and overbite had no significant changes. Conclusion: The Distal Jet appliance is effective to distalize the maxillary first molars, but promotes increase in mandibular plane angle, distal inclination, extrusion and distalization of maxillary second molars, mesialization of maxillary first premolars, proclination and protrusion of maxillary incisors, and increase in overjet, when compared to a control group.

The conventional biodiesel production process has some disadvantages. It is necessary to use refined vegetable oils with low free fatty acids (FFAs) content. An alternative route is to use low-cost acid oils in an enzymatic process. The use of lipases allows simultaneous esterification of FFAs and transesterification of triglycerides present in raw material forming alkyl esters. The aim of this work was to study the production of biodiesel using soybean oils with different acid contents (Acid Value of 8.5, 50, 90) and ethanol catalyzed by commercial immobilized lipases (Novozym 435, Lipozyme RM IM and Lipozyme TL IM). A significant decrease of acid value was observed mainly with Novozym 435 and Lipozyme RM IM. The use of a mixture of two immobilized lipases was also investigated to decrease catalyst cost and increase the amount of ester produced. The three commercial immobilized lipases were mixed in a dual system and tested for biodiesel synthesis from acid oil (AV of 8.5, 50 and 90). A positive synergistic effect occurred mainly for Lipozyme TL IM (1,3-specific lipase) and Novozym 435 (non-specific lipase) blend. The ester content doubled when this lipase mixture was used in ethanolysis of acid oil with AV of 90.

O fibroblastoma desmoplásico (FD), também conhecido como fibroma colagenoso, é uma neoplasia fibrosa benigna rara, de crescimento indolente, recentemente descrita. Ocorre predominantemente no tronco e membros superiores, poden- do surgir em qualquer idade. Os autores descrevem um caso de uma doente do sexo feminino de 42 anos de idade com FD, com compromisso neurovascular do membro superior. Foi submetida a exérese em bloco da massa tumoral e artéria braquial, com reconstrução arterial (enxerto de interposição autólogo). A análise anatomopatológica foi consistente com FD e o diagnóstico foi validado por laboratório externo internacional1. Trata-se da primeira descrição na literatura de um FD envolvendo um segmento arterial.

Desmoplastic fibroblastoma (DF), also known as collagenous fibroma, is a rare benign fibrous tumor, recently described. It occurs predominantly in trunk and upper limbs, and can appear at any age. The authors describe a case of a 42-year- old female patient with DF, with upper limb neurovascular compromise. Patient was submitted to block excision of the tumor mass and brachial artery, with arterial reconstruction (autologous interposition graft). The anatomopathological analysis was consistent with DF and the diagnosis was validated by an international external laboratory1. This is the first description in the literature of an DF involving an arterial segment.

RESUMO Este estudo tem o objetivo de descrever o perfil dos investimentos públicos em fisioterapia e verificar a correlação desses investimentos com a taxa de cobertura de plano de saúde, nas grandes regiões brasileiras e nas unidades de federação, entre 2010 e 2015. Os dados referentes aos investimentos públicos aprovados por região e unidades de federação do país segundo atendimento em fisioterapia foram obtidos no setor de Sistema de Informações Ambulatoriais do Sistema Único de Saúde, no site do Departamento de Informática do Sistema Único de Saúde (Datasus). As informações correspondentes à taxa de cobertura por plano de saúde foram obtidas no setor de Informações de Saúde Suplementar, disponível no site da Agência Nacional de Saúde Suplementar. Os dados foram analisados, e, para verificar a correlação entre a distribuição dos investimentos e a taxa de cobertura por plano de saúde, realizou-se o teste de correlação de Spearman, com nível de significância de 5,00%. A média de aplicação per capita em reais no Brasil de recursos financeiros em atendimentos em fisioterapia ao longo dos cinco anos foi de R$ 117,16 (±3,52). Dentre as regiões e para o mesmo período, a região Sul apresentou a maior média per capita (R$ 129,95 ± 5,30), seguida em ordem decrescente pelas regiões Sudeste (R$ 124,22±3,69); Nordeste (R$ 118,98±7,53); Norte (R$ 89,43±3,01) e Centro-Oeste (R$ 77,09 ± 6,54). A média de cobertura de plano privado de saúde variou de 6,20% (Acre) a 43,35% (São Paulo). Parece não haver relação entre a cobertura por plano de saúde privado e o investimento público em atendimento em fisioterapia.

ABSTRACT This study aims to describe the profile of public investments in physical therapy and to verify the correlation between these investments and the health insurance coverage rate in major Brazilian regions and Federation units, between 2010 and 2015. Data concerning the approved public investments by region and Federation units according to physical therapy attendance were obtained in the Outpatient Information System of the Unified Health System, in the website of the Department of Informatics of the Unified Health System (Datasus). The information corresponding to the health insurance coverage rate was obtained in the Supplementary Health Information sector, available on the National Supplementary Health Agency website. Data were analyzed and the Spearman correlation test was held with a significance level of 5.00% to show a correlation between investment distribution and health insurance coverage rate. The mean per capita application in Brazil, in Brazilian Reais, of financial resources in physical therapy attendances, over the five years analyzed, was R$ 117.16 (±3.52). Among the regions, and for the same period, the South region presented the highest mean per capita (R$ 129.95±5.30), followed in descending order by the Southeast (R$ 124.22±3.69), Northeast (R$ 118.98±7.53), North (R$ 89.43±3.01), and Midwest (R$ 77.09±6.54) regions. The mean coverage by private health insurance varied from 6.20% (Acre) to 43.35% (São Paulo). Apparently, no correlation exists between private health insurance coverage and public investment in physical therapy services.

RESUMEN Este estudio tiene el objetivo de describir el perfil de las inversiones públicas en fisioterapia y certificar la correlación de esas inversiones con la tasa de cobertura de seguro de salud, en las grandes regiones brasileñas y en las unidades de federación, entre 2010 y 2015. Los datos referentes a las inversiones públicas aprobadas por región y unidades de federación del país según la atención en fisioterapia fueron obtenidos en el sector de Sistema de Informaciones Ambulatorias del Sistema Único de Salud, en el sitio del Departamento de Informática del Sistema Único de Salud (Datasus). Las informaciones correspondientes a la tasa de cobertura por seguro de salud fueron obtenidas en el sector de Informaciones de Salud Suplementaria, disponible en el sitio de la Agencia Nacional de Salud Suplementaria. Los datos fueron analizados, y, para certificar la correlación entre la distribución de las inversiones y la tasa de cobertura por seguro de salud, se realizó la prueba de correlación de Spearman, con nivel de significancia del 5,00%. Y el promedio de aplicación per cápita en reales en Brasil de ingresos financieros en atenciones en fisioterapia a lo largo de los cinco años fue de R$ 117,16 (± 3,52). De entre las regiones y para el mismo período, la región Sur presentó el promedio más grande per cápita (R$ 129,95 ± 5,30), luego en orden decreciente por las regiones Sudeste (R$ 124,22 ± 3,69); Noreste (R$ 118,98 ± 7,53); Norte (R$ 89,43 ± 3,01) y Centro Oeste (R$ 77,09 ± 6,54). El promedio de cobertura de seguro privado de salud varió del 6,20% (Acre) al 43,35% (São Paulo). Parece no haber relación entre la cobertura por seguro de salud privado y la inversión pública en atención en fisioterapia.

ABSTRACT Pharmacological activities as anti-inflammatory, anti-hyperuricemic, anti-gouty arthritis, antitumor and trypanocidal activities of the aerial parts from Lychnophora trichocarpha (Spreng.) Spreng. ex Sch.Bip., Asteraceae (Brazilian arnica) have already been proved. Eremantholide C is a sesquiterpene lactone and one of the active chemical constituents responsible for these activities presented by L. trichocarpha. Therefore, the aim of this work was to develop and validate a stability indicating HPLC method for eremantholide C. Eremantholide C stability was evaluated in L. trichocarpha ethanolic extract and in its isolated form. Analytical conditions employed C18 column, acetonitrile/water in gradient elution, flow of 0.8 ml/min at 30 ºC. To correct for the loss of analyte during sample preparation the use of coumarin as an internal standard was necessary. The developed method provides good separation and resolution of the peaks, allowing quantification of eremantholide C, isolated or directly in the ethanolic extract, in internal standard presence. Validation results showed that this method is linear in the concentration range 2–180 µg/ml, precise, accurate and specific. Stability studies showed that L. trichocarpha ethanolic extract and eremantholide C remain stable for 6 months when stored at room temperature and impermeable glass bottle, therefore they can be used safely and effectively within this period. While at 40 ºC there was stability loss, at 8 ºC a stability increase was observed for the extract and the isolated eremantholide C. Forced degradation studies showed that eremantholide C degraded under acidic and alkaline conditions and was stable for three days under neutral and oxidative conditions, and when exposed to high temperature. Thus, with the development of a stability indicative method and the application of it in eremantholide C stability studies, the results can guide the development of new products that adequately preserve the original features of the biologically active substance with quality, safety and efficacy.