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ABSTRACT Objective: Patients with cystic fibrosis (CF) have a high incidence of pubertal and growth delay. In girls with CF, pubertal delay has an important psychological impact. Still, only a few studies have explored the occurrence of pubertal delay in girls with CF. The aims of this study were to compare the pubertal development of girls with CF compared with healthy controls regarding Tanner staging and pelvic ultrasound and, in girls with CF, correlate the findings with those of spirometry, body mass index, Shwachman-Kulczycki score (SKS), and genotyping. Subjects and methods: This was a cross-sectional, case-control study including 35 girls with CF aged 6-17 years and following up at the Pediatric Pulmonology Outpatient Clinic of a tertiary hospital. These patients were compared with 59 healthy controls who had undergone pelvic ultrasound as part of another study conducted by the same group. Girls with CF were consecutively enrolled in the study during their annual routine check-up visit. Data collected in the CF group included spirometry and anthropometric results, SKS values, bone age, occurrence of current cystic fibrosis-related diabetes (CFRD) and Pseudomonas aeruginosa colonization, history of meconium ileus, genotype, ultrasound parameters, and Tanner stage. Results: Pelvic ultrasound findings and Tanner stage reflected less pubertal development in girls with CF compared with healthy controls. Pubertal stage in patients with CF who presented CFRD (3.17 ± 1.16), had chronic colonization by Pseudomonas aeruginosa (3.10 ± 1.10), or were homozygous for the F508del mutation (1.91 ± 1.30) was more delayed than in controls (3.41 ± 1.41). Tanner stage correlated with age at menarche, bone age, and anthropometric and ultrasound data. Conclusions: Girls with CF presented a delay in pubertal development evaluated by Tanner stage and ultrasound parameters, which was more evident in the presence of comorbidities.

https://doi.org/10.20945/2359-3997000000404 18 downloads

Arbovirus, herpesvirus, and enterovirus associated with neurological syndromes in adult patients of a university hospital, 2017-2018
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Leon, Lucas Lopes

; Lima, Rodrigo Gonçalves de

; Boffi, Lídia Cristian

; Bindilatti, Raissa Nery

; Garlipp, Célia Regina

; Costa, Sandra Cecília Botelho

; Bonon, Sandra Helena Alves

Revista da Sociedade Brasileira de Medicina Tropical

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2021, Volume 54 elocation e0127-2021

Abstract: En | Text: En | PDF: En

Abstract INTRODUCTION: Herpesviruses, enteroviruses, and arboviruses are important because of their clinical relevance and ability to cause meningitis, encephalitis, meningoencephalitis, and other diseases. The clinical virology associated with diagnostic technologies can reduce the morbidity and mortality of such neurological manifestations. Here we aimed to identify the genomes of agents that cause neurological syndromes in cerebrospinal fluid (CSF) samples from patients with suspected nervous system infections admitted to the University Hospital of the University of Campinas, São Paulo, Brazil, in 2017-2018. METHODS: CSF samples collected from adult patients with neurological syndrome symptoms and negative CSF culture results were analyzed using polymerase chain reaction (PCR), reverse transcriptase-PCR, and real-time PCR, and their results were compared with their clinical symptoms. One CSF sample was obtained from each patient. RESULTS: Viral genomes were detected in 148/420 (35.2%) CSF samples: one of 148 (0.2%) was positive for herpes simplex virus-1; two (0.5%) for herpes simplex virus-2; eight (1.9%) for varicella-zoster virus; four (1%) for Epstein-Barr virus; one (0.2%) for cytomegalovirus; 32 (7.6%) for human herpesvirus-6; 30 (7.1%) for non-polio enterovirus; 67 (16.0%) for dengue virus, three (0.7%) for yellow fever virus, and 21 (5%) for Zika virus. CONCLUSIONS: The viral genomes were found in 35.2% of all analyzed samples, showing the high prevalence of viruses in the nervous system and the importance of using a nucleic acid amplification test to detect viral agents in CSF samples.

https://doi.org/10.1590/0037-8682-0127-2021 68 downloads

Protease and sugarcane yeast in diets for broiler chicks
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Moreira, Elvania Maria da Silva Costa

; Dourado, Leilane Rocha Barros

; Bastos, Hidaliana Paumerik Aguiar

; Ribeiro, Mabell Nery

; Silva, Sandra Regina Gomes da

; Lopes, João Batista

; Moreira Filho, Miguel Arcanjo

; Lima, Stélio Bezerra Pinheiro de

Acta Scientiarum. Animal Sciences

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2020, Volume 42 elocation e50436

Abstract: En | Text: En | PDF: En

ABSTRACT. The objective of this study was to evaluate the inclusion of protease and sugarcane yeast in poultry diets on performance, organ weight, intestinal biometry, chemical composition and deposition of body nutrients in the 1 to 7-day old phase. The experimental design consisted of a randomized complete block design, in a factorial arrangement of 2 (without and with the enzyme protease) x3 (yeast levels: 0, 6 and 12%) +1 (positive control diet), totaling seven treatments with five replicates, birds per experimental unit. The treatments used consisted of a positive control diet and the others were the negative control, with reductions in nutritional levels of protein and amino acids by 4% requirement. In the negative control diets, protease enzyme was included in three levels of sugarcane yeast. In isolation, treatments with or without inclusion of protease influenced the chemical composition of the birds. The levels of yeast from sugarcane yeast presented effects for weight gain, feed conversion, moisture and crude protein in the nutrient deposition. The inclusion of protease in diets for broiler chicks cannot remedy the poor performance provided by the use of sugarcane yeast, in addition to presenting lower levels of deposition of body nutrients.

https://doi.org/10.4025/actascianimsci.v42i1.50436 304 downloads

Chemical composition and nutrient digestibility of insect meal for broiler
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DOURADO, LEILANE R.B.

; LOPES, PATRÍCIA M.

; SILVA, VANESSA KARLA

; CARVALHO, FRANCISCA LUANA A.

; MOURA, FRANCINETE A.S.

; SILVA, LUCIANA B.

; GIANNECCHINI, LUIZ GUSTAVO

; PINHEIRO, SANDRA REGINA F.

; BIAGIOTTI, DANIEL

; KIMPARA, JANAINA M.

Anais da Academia Brasileira de Ciências

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2020, Volume 92 N. 3 elocation e20200764

Abstract: En | Text: En | PDF: En

Abstract To determine the chemical composition and digestibility of insect meal for poultry made from the larvae of Tenebrio molitor (TL) and nymphs of Gryllus assimilis (GAN) a total excreta collection test was carried out, substituting 20% of the reference diet with each type of meal. The meals presented 6074 and 5975 kcal/Kg of gross energy, with 49.34% and 52.66% protein for TL and GAN respectively. The most nutrient digestibility was less than 65%, except for energy and ether-extract digestibility in the meal from Tenebrio molitor larvae, which were over 70%. The meals under analysis can be used as a source of nutrients in poultry diets.

https://doi.org/10.1590/0001-3765202020200764 784 downloads

Metalloproteinases and colorectal cancer. Correlation of gene expression and clinical-pathological parameters
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Morini, Sandra Regina

; Denadai, Marcos Vinicius

; Waisberg, Jaques

; Lopes Filho, Gaspar de Jesus

; Matos, Delcio

; Saad, Sarhan Sydney

Acta Cirúrgica Brasileira

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2020, Volume 35 N. 7 elocation e202000707

Abstract: En | Text: En | PDF: En

Abstract Purpose: To analyze gene and protein expression of metalloproteinases 1, 2, 9, 11 and 16 and their correlation with clinicopathological variables in colorectal adenocarcinoma. Methods: A retrospective study of 114 patients with colorectal adenocarcinoma treated surgically in the period 2006 to 2008 in Hospital de Câncer de Barretos - Fundação Pio XII. The evaluation of gene expression was performed by RT-PCR, and protein by immunohistochemistry. The analysis of gene expression was classified as overexpressed genes and poorly expressed (fold change of approximately 2, p<0.05). The positivity of the markers in the immunohistochemical study was performed by semi-quantitative analysis. The tissue of TMA (Tissue Microarray) was done by two independent pathologists. Results: The gene expression validated by immuno - histochemical was MMP-1(p= 0.00 and 1.57 fold change) and MMP – 2 (p= 0.01 and – 1.84 to fold change) when correlated with the histological types mucinous and adenocarcinoma NOS, MMP9 (p=0.01 and fold change of 1.13) and MMP-16 (p=0.03 and 1.61 fold change) when compared with the histological types villous and adenocarcinoma NOS, MMP - 11 statistically significant in relation to male (p = 0.04 and 1.65 fold change). Conclusions: The MMPs 1, 2, 9, 11 and 16 gene and protein expression with statistical significance in at least one of the clinicopathological variables studied. Thus, we conclude that these MMPs have potential as a prognostic factor in colorectal adenocarcinoma.

https://doi.org/10.1590/s0102-865020200070000007 751 downloads

Guideline of the Brazilian Society of Cardiology on Telemedicine in Cardiology - 2019
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Lopes, Marcelo Antônio Cartaxo Queiroga

; Oliveira, Gláucia Maria Moraes de

; Ribeiro, Antonio Luiz Pinho

; Pinto, Fausto J.

; Rey, Helena Cramer Veiga

; Zimerman, Leandro Ioschpe

; Rochitte, Carlos Eduardo

; Bacal, Fernando

; Polanczyk, Carisi Anne

; Halperin, Cidio

; Araújo, Edson Correia

; Mesquita, Evandro Tinoco

; Arruda, José Airton

; Rohde, Luis Eduardo Paim

; Grinberg, Max

; Moretti, Miguel

; Caramori, Paulo Ricardo Avancini

; Botelho, Roberto Vieira

; Brandão, Andréa Araújo

; Hajjar, Ludhmila Abrahão

; Santos, Alexandre Fonseca

; Colafranceschi, Alexandre Siciliano

; Etges, Ana Paula Beck da Silva

; Marino, Bárbara Campos Abreu

; Zanotto, Bruna Stella

; Nascimento, Bruno Ramos

; Medeiros, Cesar Rocha

; Santos, Daniel Vitor de Vasconcelos

; Cook, Daniela Matos Arrowsmith

; Antoniolli, Eduardo

; Souza Filho, Erito Marques de

; Fernandes, Fábio

; Gandour, Fabio

; Fernandez, Francisco

; Souza, Germano Emilio Conceição

; Weigert, Guilherme de Souza

; Castro, Iran

; Cade, Jamil Ribeiro

; Figueiredo Neto, José Albuquerque de

; Fernandes, Juliano de Lara

; Hadlich, Marcelo Souza

; Oliveira, Marco Antonio Praça

; Alkmim, Maria Beatriz

; Paixão, Maria Cristina da

; Prudente, Maurício Lopes

; Aguiar Netto, Miguel A. S.

; Marcolino, Milena Soriano

; Oliveira, Monica Amorim de

; Simonelli, Osvaldo

; Lemos Neto, Pedro A.

; Rosa, Priscila Raupp da

; Figueira, Renato Minelli

; Cury, Roberto Caldeira

; Almeida, Rodrigo Coelho

; Lima, Sandra Regina Franco

; Barberato, Silvio Henrique

; Constancio, Thiago Inocêncio

; Rezende, Wladimir Fernandes de

Arquivos Brasileiros de Cardiologia

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nov 2019, Volume 113 N. 5 Pages 1006 - 1056

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.5935/abc.20190205 5274 downloads

Recommendations of the Brazilian Society of Rheumatology for the diagnosis and treatment of chikungunya fever. Part 2 - Treatment
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Marques, Claudia Diniz Lopes

; Duarte, Angela Luzia Branco Pinto

; Ranzolin, Aline

; Dantas, Andrea Tavares

; Cavalcanti, Nara Gualberto

; Gonçalves, Rafaela Silva Guimarães

; Rocha Junior, Laurindo Ferreira da

; Valadares, Lilian David de Azevedo

; Melo, Ana Karla Guedes de

; Freire, Eutilia Andrade Medeiros

; Teixeira, Roberto

; Neto, Francisco Alves Bezerra

; Medeiros, Marta Maria das Chagas

; Carvalho, Jozélio Freire de

; Santos, Mario Sergio F.

; Océa, Regina Adalva de L. Couto

; Levy, Roger A.

; Andrade, Carlos Augusto Ferreira de

; Pinheiro, Geraldo da Rocha Castelar

; Abreu, Mirhelen Mendes

; Verztman, José Fernando

; Merenlender, Selma

; Ribeiro, Sandra Lucia Euzebio

; Costa, Izaias Pereira da

; Pileggi, Gecilmara

; Trevisani, Virginia Fernandes Moça

; Lopes, Max Igor Banks

; Brito, Carlos

; Figueiredo, Eduardo

; Queiroga, Fabio

; Feitosa, Tiago

; Tenório, Angélica da Silva

; Siqueira, Gisela Rocha de

; Paiva, Renata

; Vasconcelos, José Tupinambá Sousa

; Christopoulos, Georges

Revista Brasileira de Reumatologia

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2017, Volume 57 Suppl. 2 Pages s438 - s451

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A febre chikungunya tem se tornado um importante problema de saúde pública nos países onde ocorrem as epidemias, visto que metade dos casos evolui com artrite crônica, persistente e incapacitante. Os dados na literatura sobre terapêuticas específicas nas diversas fases da artropatia ocasionada pela infecção pelo vírus chikungunya (CHIKV) são limitados, não existem estudos randomizados de qualidade que avaliem a eficácia das diferentes terapias. Há algumas poucas publicações sobre o tratamento das manifestações musculoesqueléticas da febre chikungunya, porém com importantes limitações metodológicas. Os dados atualmente disponíveis não permitem conclusões favoráveis ou contrárias a terapêuticas específicas, bem como uma adequada avaliação quanto à superioridade entre as diferentes medicações empregadas. O objetivo deste trabalho foi elaborar recomendações para o tratamento da febre chikungunya no Brasil. Foi feita uma revisão da literatura com seleção de artigos baseados em evidência, nas bases de dados Medline, SciELO, PubMed e Embase e de resumos de anais de congressos, além da opinião dos especialistas para dar apoio às decisões tomadas para definir as recomendações. Para a definição do grau de concordância foi feita uma metodologia Delphi, em duas reuniões presenciais e várias rodadas de votação on line. Este artigo refere-se à parte 2 das Recomendações da Sociedade Brasileira de Reumatologia para Diagnóstico e Tratamento da Febre Chikungunya, que trata especificamente do tratamento.

Abstract Chikungunya fever has become an important public health problem in countries where epidemics occur because half of the cases progress to chronic, persistent and debilitating arthritis. Literature data on specific therapies at the various phases of arthropathy caused by chikungunya virus (CHIKV) infection are limited, lacking quality randomized trials assessing the efficacies of different therapies. There are a few studies on the treatment of musculoskeletal manifestations of chikungunya fever, but these studies have important methodological limitations. The data currently available preclude conclusions favorable or contrary to specific therapies, or an adequate comparison between the different drugs used. The objective of this study was to develop recommendations for the treatment of chikungunya fever in Brazil. A literature review was performed via evidence-based selection of articles in the databases Medline, SciELO, PubMed and Embase and conference proceedings abstracts, in addition to expert opinions to support decision-making in defining recommendations. The Delphi method was used to define the degrees of agreement in 2 face-to-face meetings and several online voting rounds. This study is part 2 of the Recommendations of the Brazilian Society of Rheumatology (Sociedade Brasileira de Reumatologia - SBR) for the Diagnosis and Treatment of chikungunya fever and specifically addresses treatment.

https://doi.org/10.1016/j.rbre.2017.06.004 38509 downloads

10.

Recommendations of the Brazilian Society of Rheumatology for diagnosis and treatment of Chikungunya fever. Part 1 - Diagnosis and special situations
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Marques, Claudia Diniz Lopes

; Duarte, Angela Luzia Branco Pinto

; Ranzolin, Aline

; Dantas, Andrea Tavares

; Cavalcanti, Nara Gualberto

; Gonçalves, Rafaela Silva Guimarães

; Rocha Junior, Laurindo Ferreira da

; Valadares, Lilian David de Azevedo

; Melo, Ana Karla Guedes de

; Freire, Eutilia Andrade Medeiros

; Teixeira, Roberto

; Bezerra Neto, Francisco Alves

; Medeiros, Marta Maria das Chagas

; Carvalho, Jozélio Freire de

; Santos, Mario Sergio F.

; Océa, Regina Adalva de L. Couto

; Levy, Roger A.

; Andrade, Carlos Augusto Ferreira de

; Pinheiro, Geraldo da Rocha Castelar

; Abreu, Mirhelen Mendes

; Verztman, José Fernando

; Merenlender, Selma

; Ribeiro, Sandra Lucia Euzebio

; Costa, Izaias Pereira da

; Pileggi, Gecilmara

; Trevisani, Virginia Fernandes Moça

; Lopes, Max Igor Banks

; Brito, Carlos

; Figueiredo, Eduardo

; Queiroga, Fabio

; Feitosa, Tiago

; Tenório, Angélica da Silva

; Siqueira, Gisela Rocha de

; Paiva, Renata

; Vasconcelos, José Tupinambá Sousa

; Christopoulos, Georges

Revista Brasileira de Reumatologia

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2017, Volume 57 Suppl. 2 Pages s421 - s437

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo A febre chikungunya tem se tornado um importante problema de saúde pública nos países onde ocorrem as epidemias. Até 2013, as Américas haviam registrado apenas casos importados quando, em outubro desse mesmo ano, foram notificados os primeiros casos na Ilha de Saint Martin, no Caribe. No Brasil, os primeiros relatos autóctones foram confirmados em setembro de 2014 e até a semana epidemiológica 37 de 2016 já haviam sido registrados 236.287 casos prováveis de infecção pelo chikungunya vírus (CHIKV), 116.523 confirmados sorologicamente. As mudanças ambientais causadas pelo homem, o crescimento urbano desordenado e o número cada vez maior de viagens internacionais têm sido apontados como os fatores responsáveis pela reemergência de epidemias em grande escala. Caracterizada clinicamente por febre e dor articular na fase aguda, em cerca de metade dos casos existe evolução para a fase crônica (além de três meses), com dor persistente e incapacitante. O objetivo deste trabalho foi elaborar recomendações para diagnóstico e tratamento da febre chikungunya no Brasil. Para isso, foi feita revisão da literatura nas bases de dados Medline, SciELO e PubMed, para dar apoio às decisões tomadas para definir as recomendações. Para a definição do grau de concordância foi feita uma metodologia Delphi, em duas reuniões presenciais e várias rodadas de votação on line. Foram geradas 25 recomendações, divididas em três grupos temáticos: (1) diagnóstico clínico, laboratorial e por imagem; (2) situações especiais e (3) tratamento. Na primeira parte estão os dois primeiros temas e o tratamento na segunda.

Abstract Chikungunya fever has become a relevant public health problem in countries where epidemics occur. Until 2013, only imported cases occurred in the Americas, but in October of that year, the first cases were reported in Saint Marin island in the Caribbean. The first autochthonous cases were confirmed in Brazil in September 2014; until epidemiological week 37 of 2016, 236,287 probable cases of infection with Chikungunya virus had been registered, 116,523 of which had serological confirmation. Environmental changes caused by humans, disorderly urban growth and an ever-increasing number of international travelers were described as the factors responsible for the emergence of large-scale epidemics. Clinically characterized by fever and joint pain in the acute stage, approximately half of patients progress to the chronic stage (beyond 3 months), which is accompanied by persistent and disabling pain. The aim of the present study was to formulate recommendations for the diagnosis and treatment of Chikungunya fever in Brazil. A literature review was performed in the MEDLINE, SciELO and PubMed databases to ground the decisions for recommendations. The degree of concordance among experts was established through the Delphi method, involving 2 in-person meetings and several online voting rounds. In total, 25 recommendations were formulated and divided into 3 thematic groups: (1) clinical, laboratory and imaging diagnosis; (2) special situations; and (3) treatment. The first 2 themes are presented in part 1, and treatment is presented in part 2.

https://doi.org/10.1016/j.rbre.2017.05.006 37996 downloads

11.

Documentação do processo de enfermagem: justificativa e métodos de estudo analítico
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Cruz, Diná de Almeida Lopes Monteiro da

; Guedes, Erika de Souza

; Santos, Mariana Alvina dos

; Sousa, Regina Márcia Cardoso de

; Turrini, Ruth Natalia Teresa

; Maia, Magda Maria

; Araújo, Sandra Alves Neves

Revista Brasileira de Enfermagem

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feb 2016, Volume 69 N. 1 Pages 197 - 204

Abstract: En Es Pt | Text: En Es Pt | PDF: En | PDF: Es | PDF: Pt

ABSTRACT Objective: to describe the methods used to analyze the associations between variables of service, nursing and the nursing process documentation in institutions of the Department of Health of the State of São Paulo. Method: multilevel analytical study with data obtained in the domains of institution, units of the institution and nursing professionals who work there, using standardized instruments. The analyses had as axis the degree of completeness of the nursing process documentation in units or institutions and their association with variables of nursing personnel, of units and of institutions. Conclusion: This study will provide important empirical evidence on the factors involved in the nursing process documentation.

RESUMEN Objetivo: describir los métodos usados para analizar las asociaciones entre variables del servicio, de la enfermería y de la documentación del proceso de enfermería en las instituciones de la Secretaria de Estado de la Salud de São Paulo. Método: estudio analítico multi-nivel, con datos obtenidos en los ámbitos de la institución, de los sectores de la institución y de los profesionales de enfermería que en ellos actúan, por medio de instrumentos empadronados. Los análisis tuvieron como eje el grado de la documentación completa del proceso de enfermería en los sectores o en las instituciones y sus asociaciones con variables del personal de enfermería, de las unidades y de las instituciones. Conclusión: este estudio empírico fornecerá importantes evidencias sobre los factores envueltos en la documentación del proceso de enfermería.

RESUMO Objetivo: descrever os métodos usados para analisar as associações entre variáveis do serviço, da enfermagem e da documentação do processo de enfermagem nas instituições da Secretaria de Estado da Saúde de São Paulo. Método: estudo analítico multinível, com dados obtidos nos âmbitos da instituição, dos setores da instituição e dos profissionais de enfermagem que neles atuam, por meio de instrumentos padronizados. As análises tiveram como eixo o grau de completude da documentação do processo de enfermagem nos setores ou nas instituições e suas associações com variáveis do pessoal de enfermagem, das unidades e das instituições. Conclusão: este estudo empírico fornecerá importantes evidências sobre os fatores envolvidos na documentação do processo de enfermagem. Descritores: Processos de Enfermagem; Pesquisa Metodológica em Enfermagem; Pesquisa em Enfermagem.

https://doi.org/10.1590/0034-7167.2016690126i 2 downloads

12.

Documentação do processo de enfermagem: justificativa e métodos de estudo analítico
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Cruz, Diná de Almeida Lopes Monteiro da

; Guedes, Erika de Souza

; Santos, Mariana Alvina dos

; Sousa, Regina Márcia Cardoso de

; Turrini, Ruth Natalia Teresa

; Maia, Magda Maria

; Araújo, Sandra Alves Neves

Revista Brasileira de Enfermagem

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feb 2016, Volume 69 N. 1 Pages 197 - 204

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

https://doi.org/10.1590/0034-7167.2016690126i 35808 downloads

13.

Ultrassonografia abdominal de tamanduás-bandeira (Myrmecophaga tridactyla Linnaeus, 1758) mantidos em cativeiro
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Lopes, Érika R.

; Morgado, Thaís O.

; Meireles, Yara S.

; Jorge, Adriano A.

; Zago, Arielli A.Q.

; Corrêa, Sandra H.R.

; Paz, Regina C.R. da

; Néspoli, Pedro B.

Pesquisa Veterinária Brasileira

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nov 2015, Volume 35 N. 11 Pages 919 - 924

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A ultrassonografia é um método de imagem não invasivo e uma ferramenta importante para o diagnóstico de uma variedade de enfermidades de animais. Neste trabalho foi realizada a técnica de ultrassonografia transabdominal em cinco tamanduás-bandeira (Myrmecophaga tridactyla), dois machos e três fêmeas, pertencentes ao Zoológico da Universidade Federal de Mato Grosso e acompanhada a necrópsia de um animal morto de causas naturais. Os animais do zoológico foram anestesiados com Tiletamina e Zolazepam (Zoletil®) e mantidos em plano anestésico com Isofluorano. Foram realizadas varreduras com transdutor linear (LA332) multifrequencial de 3,0 a 11 MHz do fígado, vesícula biliar, estômago, baço, rins, bexiga e testículos. Os resultados obtidos mostram que existem semelhanças entre a arquitetura esplênica, a textura e ecogenicidade hepática, a posição e a aparência ultrassonográfica da vesícula biliar quando comparado com a dos caninos. Existem diferenças como localização renal, localização dos testículos, espessura da parede do estômago e presença de liquido livre anecóico entre o estômago, baço e rim esquerdo em todos os animais estudados.

ABSTRACT: Ultrasonography is a method of non-invasive imaging and an important tool for diagnosing a variety of animal diseases. In this paper the technique of transabdominal ultrasonography in five anteaters (Myrmecophaga tridactyla), two males and three females, belonging to the Federal University of Mato Grosso Zoo and a necropsy of an animal dead for natural cause were performed. The Zoo animals were anesthetized with tiletamine and zolazepan (Zoletil®) and maintained with isoflurane. Linear multifrequency transducer (LA 332) of the 3.0 to 11 MHz scans of the liver, gallbladder, stomach, spleen, kidneys, bladder and testes. There are similarities between the splenic architecture, the liver texture and echogenicity and the gallbladder ultrassonografic position and appearance when compared to the canines. Differences exist as renal and testes location, stomach wall thickness and presence of free anechoic fluid among the stomach, spleen and left kidney in all animals studied.

https://doi.org/10.1590/S0100-736X2015001100008 3569 downloads

14.

Desempenho, morfometria duodenal e histopatologia do fígado de frangos de corte alimentados com dietas contendo diferentes níveis de selênio orgânico em condições de estresse calórico
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CARVALHO, Genilson Bezerra de

; LOPES, João Batista

; SILVA, Sandra Regina Gomes da

; DOURADO, Leilane Rocha Barros

; MIRANDA, Dayane Francisca Higino

; COSTA, Francisco Assis Lima

Revista Brasileira de Saúde e Produção Animal

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jun 2015, Volume 16 N. 2 Pages 365 - 376

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

A pesquisa foi desenvolvida para avaliar o desempenho, a morfometria duodenal e as alterações histopatológicas do fígado de frangos de corte, alimentados com dietas contendo diferentes níveis de selênio (0,5; 1,1; 1,7; 2,3; 2,9 e 3,5ppm de Se/kg de ração), criados no período de 1 a 21 dias de idade, em condições de estresse calórico. As aves receberam dietas isonutritivas à base de milho e farelo de soja, formuladas para atender as exigências nutricionais, exceto para o selênio. A partir de uma dieta basal foi feita a suplementação do selênio, com o uso de selenometionina (Sel-Plex®). Setecentos e vinte pintos da linhagem Ross 308, mistos, foram utilizados em delineamento de blocos casualizados, com seis tratamentos e seis repetições com vinte aves por unidade experimental. O ambiente interno dos galpões foi avaliado com base no Índice de Temperatura de Globo Negro e Umidade(ITGU),no Índice de Temperatura e Umidade(ITU)e Temperatura Efetiva(TE). O uso da suplementação de selênio orgânico na alimentação de frangos de corte melhora a conversão alimentar, mas, não interfere nos parâmetros comprimento de cripta e altura de vilosidade e não promove reação inflamatória no fígado destes, nos níveis testados.

The research was conducted to evaluate the performance, duodenal morphology and histopathological changes in the liver of broilers fed diets containing different levels of selenium (0.5, 1.1, 1.7, 2.3, 2, 9 and 3.5ppm Se / kg diet), created in the period 1-21 days old, under heat stress conditions. The birds were isonutritives diets based on corn and soybean meal, formulated to meet the nutritional requirements, except for selenium. From a basic diet supplementation of selenium was taken, such as use of selenomethionine (Sel - Plex®). Seven hundred and twenty chicks of mixed lineage Ross 308, were used in a randomized complete block design with six treatments and six replicates of twenty birds each. The internal environment of the sheds was evaluated based on the Temperature Index Black Globe Humidity (BGT), the Temperature and Humidity Index (THI) and Effective Temperature (ET). The use of organic selenium supplementation in the diet of broilers improves feed conversion, but does not interfere in the length parameter crypt and villus height and does not promote inflammation in the liver of these, in both levels.

https://doi.org/10.1590/S1519-99402015000200011 1599 downloads

15.

Solubilization of diabase and phonolite dust by filamentous fungus
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Brandão, Juliana Andréia Vrba

; Lopes-Assad, Maria Leonor Ribeiro Casimiro

; Ceccato-Antonini, Sandra Regina

Revista Ceres

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oct 2014, Volume 61 N. 5 Pages 740 - 745

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Este trabalho objetivou avaliar o efeito do fungo Aspergillus niger, linhagem CCT4355, na liberação de nutrientes contidos em pós de dois tipos de rocha (fonolito e diabásio), por meio de ensaios de solubilização in vitro. O delineamento experimental foi o inteiramente casualizado, em esquema fatorial 5x4, com três repetições. Foram avaliados cinco tratamentos (pó de fonolito + meio; pó de fonolito + fungo + meio, pó de diabásio + meio, pó de diabásio + fungo + meio e fungo + meio) e quatro épocas de amostragens (0, 10, 20 e 30 dias). Adicionou-se pó de rocha (0,4% m/v) a Erlenmeyers de 125 mL, com 50 mL de meio de cultura líquido adaptado para A. niger. Os frascos foram incubados a 30 °C, por 30 dias, e foram feitas análises de pH em água, acidez titulável e teores de potássio, cálcio, magnésio, zinco, ferro e manganês solúveis. O A. niger foi capaz de produzir ácidos orgânicos que solubilizaram íons. Este resultado indica o potencial de A. niger para alterar minerais contidos em pós de rocha, com capacidade de interagir de diferentes formas com os nutrientes. Houve aumento significativo da quantidade de K solúvel, no tratamento com pó de fonolito, em presença do fungo. Conclui-se que a linhagem CCT4355 de A. niger pode solubilizar minerais contidos nesses pós de rochas.

The objective of this study was to evaluate the effect of the fungus Aspergillus niger strain CCT4355 in the release of nutrients contained in two types of rock powder (diabase and phonolite) by means of in vitro solubilization trials. The experimental design was completely randomized in a 5 x 4 factorial design with three replications. It was evaluated five treatments (phonolite dust + culture medium; phonolite dust + fungus + culture medium; diabase powder + culture medium; diabase powder + fungus + culture medium and fungus + culture medium) and four sampling dates (0, 10, 20 and 30 days). Rock dust (0.4% w/v) was added to 125 mL Erlenmeyer flasks containing 50 mL of liquid culture medium adapted to A. niger. The flasks were incubated at 30°C for 30 days, and analysis of pH (in water), titratable acidity, and concentrations of soluble potassium, calcium, magnesium, zinc, iron and manganese were made. The fungus A. niger was able to produce organic acids that solubilized ions. This result indicates its potential to alter minerals contained in rock dust, with the ability to interact in different ways with the nutrients. A significant increase in the amount of K was found in the treatment with phonolite dust in the presence of the fungus. The strain CCT4355 of A. niger can solubilize minerals contained in these rocks dust.

https://doi.org/10.1590/0034-737X201461050018 1674 downloads

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