RESUMO O objetivo deste trabalho foi avaliar a composição química da água residuária da suinocultura (ARS) e efeito nos atributos químicos do solo e planta em um experimento de longa duração. A área destina-se a 8 anos a produção de feno de capim Tifton 85 (Cynodon spp.), com aplicação média anual de 360 m3 ha-1 dividida em 6 aplicações. Também se avaliou a composição química do solo em duas profundidades 0,0-0,20 e 0,20-0,40 m de profundidade e a composição química da planta e material vegetal sobre o solo, avaliada em duas épocas do ano (novembro e junho). As concentrações de nitrogênio, cálcio e cobre da ARS não reduziram do afluente até a lagoa de armazenamento. Ressalta-se que os teores de Cu, Zn e Mn foram classificados como em excesso no solo, que pode ser consequência dos elevados níveis destes nutrientes na ração dos suínos na granja fornecedora da ARS, principalmente na ração de leitões aliado a baixa extração destes nutrientes pelas plantas. Com relação aos micronutrientes e o fósforo (46,08 mg dm-3) no solo estes deverão ser avaliados em profundidades maiores, superiores ao presente estudo em função das concentrações não variarem muito entre profundidades e se mostrarem elevadas. As concentrações de cobre e zinco foram elevadas na parte aérea das plantas com médias de 133,0 e 139,30 mg kg-1, respectivamente. O uso contínuo de ARS deverá ter monitoramento contínuo através de análises de solo e do dejeto e alternativas devem ser buscadas no sentido de reduzir a concentração de micronutrientes e fósforo na ARS.

ABSTRACT The aim of this study was to evaluate the chemical composition of swine wastewater (SW), and its effect on the chemical attributes of soil and plants in a long-term experiment. The area was designed for an 8-year hay production, using Tifton 85 grass (Cynodon spp.), with an average annual application of 360 m3 ha-1, divided into 6 applications. Also, soil chemical composition was evaluated at 0.0-0.20 and 0.20-0.40 m of depth, and the chemical composition of plant and vegetable materials on the ground were evaluated for two months (November and June). Concentrations of N, Ca, and Cu in SW did not decrease from the affluent to the storage lagoon. It was observed that the content of Cu, Zn, and Mn was classified as in excess in soil that could be a consequence of high levels of these micronutrients utilized in rations of swine in the SW supplier farm, mainly in the rations of piglets combined with a low extraction of these nutrients by plants. In relation to micronutrients and P (46.08 mg dm-3) in the soil, these should be evaluated in greater depth than performed in the present study, because the concentrations did not vary considerably among depths and were high. The concentrations of Cu and Zn were high in shoot plants with an average of 133.0 and 139.30 mg kg-1, respectively. The continuous use of SW should be monitored continuously through analyses of soil and waste, and alternatives must be researched to reduce the concentration of micronutrients and P in SW.

RESUMO: O objetivo do presente trabalho foi quantificar e identificar a ocorrência dos principais gêneros de fungos, no solo, material vegetal morto, raiz e parte aérea no capim Tifton 85, adubado com biofertilizante suíno, 7 dias após o início da rebrota. As avaliações foram realizadas, durante seu desenvolvimento (15; 22; 29 e 36 dias após o início da rebrota), no momento do enfardamento e no feno, com 30 dias de armazenamento. O delineamento experimental foi em blocos casualizados com parcelas subdivididas no tempo, com quatro locais de coleta no campo de produção de feno (solo, parte aérea da planta, raiz e material vegetal morto), e 6 tempos de avaliação para parte aérea da planta (15; 22; 29; e 36 dias início da rebrota, no momento do enfardamento e com 30 dias de armazenamento) e quatro tempos de avaliação para solo, raiz e material vegetal morto (15; 22; 29; e 36 dias após início da rebrota). Na contagem e identificação dos gêneros dos fungos referentes a cada parte da planta e solo, foram calculadas as médias e essas submetidas à análise descritiva. Uma maior população fúngica foi observada no material vegetal morto e solo, predominando o gênero Penicillium em todas as partes da planta estudadas.

ABSTRACT: The objective of this study was to quantify and identify the occurrence of the main genres of fungi soil, dead plant material, roots and shoots in Tifton 85 fertilized with swine biofertilizer, 7 days after start of regrowth. The evaluation were carried out during development (15, 22, 29 and 36 days of age) at the time of baling, hay and 30 days of storage. The experimental design was randomized blocks with split plots in time sub with 4 sampling sites (soil, plant tops, roots and dead plant material), and 6 times of evaluation for aerial part of the plant (15, 22, 29 and 36 days of regrowth, at the time of baling and 30 days of storage) and 4-cycle assessment for soil, roots and dead plant material (15, 22, 29 and 36 days of regrowth). In the counting and identification of genera of fungi belonging to each part of the plant and soil were averaged and those submitted to descriptive analysis. Greater fungal population was observed in soil and dead plant material, predominantly Penicillium in all parts of the plant studied.

Objetivou-se estudar o armazenamento do resíduo da extração de amido da mandioca, sem a utilização de tecnologias, por meio da avaliação química, valores de pH, temperatura, desenvolvimento de microrganismos e micotoxinas. O delineamento utilizado foi em blocos casualizados com oito tratamentos (diferentes dias de estocagem: 0, 3, 6, 9, 12, 15, 18 e 21) e cinco repetições. Houve diferença significativa para o teor de MS em função dos dias de estocagem. Obteve-se efeito linear negativo para os valores de pH, os quais decresceram com os dias de armazenamento. Houve significância do período de estocagem apenas sobre a população de fungos e leveduras, que se elevou até os 17 dias de estocagem, com posterior redução, micotoxinas foram detectadas no resíduo da extração do amido da mandioca. Apesar de não apresentar alterações na composição química, a estocagem do resíduo da extração do amido da mandioca por 21 dias demonstrou ser um processo de conservação ineficiente, devido ao desenvolvimento de fungos e presença de micotoxinas

The purpose of this paper was to study the storage of the residue from the extraction of cassava starch without the use of storage technologies, through chemical evaluation, pH values, temperature, development of microorganisms and mycotoxins. A randomized block design was used with eight treatments (different storage periods: 0, 3, 6, 9, 12, 15, 18 and 21 days) and five replications. There was a significant difference for DM content, as a function of days in storage. The chemical composition of the residue from the extraction of cassava starch did not changed throughout the storage period. A negative linear effect was obtained for the pH values, which decreased with days in storage. There was significance of the storage period only for the fungus and yeast population, which increased up to 17 days of storage, with subsequent reduction. Mycotoxins were detected in the residue from the extraction of cassava starch. Despite it did not showed changes in the chemical composition the storage of residue from the extraction of cassava starch for 21 days proved to be an inefficient preservation process, due to the development of molds and mycotoxins

O uso de silagem de gramíneas trópicas tem se tornado comum na alimentação de ruminante. Conduziu-se um experimento com o objetivo de avaliar o perfil fermentativo e valor nutricional da silagem de capim Tifton 85 com diferentes aditivos e emurchecimento. Os tratamentos foram: pré-secagem ao sol por 2 horas antes de ensilar, uso de inoculante bacteriano-enzimático, adição de casca de soja, adição de quirera de milho e uso de sal grosso na camada superficial do silo. O delineamento experimental foi inteiramente casualizado, com 6 tratamentos e 4 repetições. Plantas de capim Tifton 85 com 38 dias de crescimento foram ensiladas em silos experimentais com válvula tipo Bunsen com densidades de compactação de 236 kg de silagem por m³ para o Tifton 85 pré-seco ao sol e 294 kg de silagem por m³ para os demais tratamentos. As proporções de casca de soja e quirera de milho, adicionados à silagem, foram calculados com base no teor de MS inicial do Tifton 85 visando à obtenção de MS de 320 g kg-1 para o material a ser ensilado. A capacidade tampão não diferiu entre tratamentos ficando em 29,56 (meqHCl/100gMS). Verificou-se que os teores de nitrogênio amoniacal não diferiram entre as silagens e mantiveram-se baixos (32,2 g kg-1), o pH após a abertura dos silos também não diferiu com média de 4,09 ficando acima de 4,2 apenas na silagem de Tifton 85 sem pré-tratamento. Os teores de proteína bruta foram mais elevados nas silagens de capim Tifton 85 e capim Tifton 85 com casca de soja (174,8 g kg-1). O uso de quirera de milho promoveu redução nos valores de FDN. O uso de sal na camada superficial reduziu a produção de ácido acético e lático.

The use of tropical grasses silage has become common in ruminant feed. An experiment was conducted to evaluate the fermentation characteristics, nutritional value, pH, fermentative capacity, ammonia nitrogen / total nitrogen (NH3N/total N) of Tifton 85 bermudagrass grass silage with different additives and wilting. The treatments were: pre-drying in the sun for two hours before silage, use of inoculant-enzymatic addition of soybean hulls, corn grits addition and use of salt in the surface layer of the silo. The experimental design was completely randomized with 6 treatments and 4 replications. Plants of Tifton 85 bermudagrass with 38 days of growth were ensiled in experimental silos with Bunsen valve type with packing densities of 236 kg of silage per m³ for Tifton 85 bermudagrass pre-dried in the sun and 294 kg of silage per m³ for the other treatments. The proportions of soybean hulls and corn grits added to the silage were calculated based on the initial DM content of Tifton 85 bermudagrass order to obtain MS 320 g kg-1 for the material to be ensiled. The buffering capacity did not differ between treatments in getting 29.56 (meqHCl/100gMS). It was found that the concentration of ammonia nitrogen did not differ between silages and remained low (3.22 g kg-1), the pH after silo opening was also similar with an average of 4.09 getting above 4.2 only in Tifton 85 bermudagrass silage without pre-treatment. The crude protein was higher in silages Tifton 85 bermudagrass and Tifton 85 bermudagrass with soybean hulls (17.48 g kg-1). The use corn grits caused a reduction in the values of NDF. The use of salt on the surface the layer reduced the production of latic and acetic acid.

J.P. Ames, M.A. Neres, D.D. Castagnara, L.M. Mufatto, C. Ducati, C.C. Jobim, and T.T. Tres. 2014. Dry matter production, chemical composition, dry matter digestibility and occurrence of fungi in Bermuda grass hay (Cynodon dactylon) under different fertilization systems or associated with pea plantings in winter. Cien. Inv. Agr. 41(2): 163-174. This study aimed to evaluate the structural characteristics, dehydration curve, DM production, chemical composition, in vitro dry matter digestibility and occurrence of fungi in Bermuda grass hay (Cynodon dactylon cv. 'Bermuda grass', Tifton 85) produced in winter under different forms of fertilization or in association with a winter annual legume. The experimental design used was a randomized block with split plots in time and with four treatments: Bermuda grass without fertilization or intercropping, Bermuda grass with nitrogen (N) chemical fertilizer (100 kg N ha-'year-1), Bermuda grass oversown with forage pea (Pisum arvense cv. 'Iapar 83'), and Bermuda grass with the addition of 70 m³ ha-1 swine slurry. Three evaluation periods (cutting, baling and 30 days of storage) and five replicates were used. The DM yield of Bermuda grass without N was 2607 kg ha-1. The use of swine slurry increased the DM yield of Bermuda grass more than the use of the N chemical fertilizer (4864 and 3551 kg ha-1, respectively). In association with forage pea, a high total DM yield was obtained: 4261 kg ha-1 of pea and 2171 kg ha-1 of Bermuda grass. The dehydration time and final crude protein content of the Bermuda grass were higher in association with the legume. The levels of acid detergent-insoluble protein increased with storage. The in vitro DM digestibility reduced the cut to 30 days of storage in treatments with Bermuda grass without association with the legume. A higher occurrence of fungi occurred after 30 days of storage, with Penicillium generally predominant; however, Phoma was predominant in the hay produced from Bermuda grass grown with no N supplementation.

J.P. Ames, M.A. Neres, D.D. Castagnara, L.M. Mufatto, C. Ducati, C.C. Jobim y T.T. Tres. 2014. Producción de materia seca, composición química, digestibilidad de la materia seca y la aparición de hongos en heno de pasto Bermuda (Cynodon dactylon) bajo diferentes sistemas de fertilización o asociado con leguminosa anual de invierno. Cien. Inv. Agr. 41(2): 163-174. Este estudio tuvo como objetivo la evaluación de la curva de características estructurales, la deshidratación, la materia seca (MS), la composición química, digestibilidad in vitro de la MS y la aparición de hongos en heno de pasto Bermuda (Cynodon dactylon, cv. 'Tifton 85'), producido en invierno, bajo diferentes formas de fertilización o en asociación con una leguminosa anual de invierno. El diseño experimental utilizado fue de bloques al azar con parcelas divididas en el tiempo con cuatro tratamientos: Pasto Bermuda sin fertilización o consorcio, pasto Bermuda con fertilizante químico de nitrógeno (100 kg N ha- ¹ año -1), pasto Bermuda en asociación con el legume (Pisium avarse 'Iapar 83'), y pasto Bermuda con la adición de 70 m³ de purines ha-1 durante tres periodos de evaluación (de corte, de balas y 30 días de almacenamiento), con cinco repeticiones. El rendimiento de MS de pasto Bermuda sin nitrógeno fue 2.607 kg ha-1. El uso de purines porcinos aumentó el rendimiento de MS de pasto Bermuda más que el uso del nitrógeno fertilizante químico (4864 y 3551 kg ha-1, respectivamente). Su asociación con la leguminosa, de alto rendimiento de MS total, obtuvo: 4.261 kg ha-1 de guisantes y 2.171 kg ha-1 de pasto Bermuda. El tiempo de deshidratación y de proteína cruda total del heno de pasto Bermuda fueron más altos en asociación con leguminosas. Los niveles de la proteína insoluble en detergente ácido aumentaron con el almacenamiento y la digestibilidad de la MS in vitro, reduciendo el corte a los 30 días de almacenamiento en los tratamientos con pasto Bermuda y sin asociación con leguminosas. La mayor incidencia de los hongos se produjo después de 30 días de almacenamiento, con un predominio de Penicillium, excepto en el heno de pasto Bermuda, en ausencia de nitrógeno, donde el género predominante fue Phoma.

The objective of this study was to evaluate the microbiological profile and aerobic stability of silage with Tifton 85 bermudagrass and different additives and wilting. The studied treatments were: pre-drying in the sun for two hours before ensiling; use of bacterial-enzymatic inoculant; addition of soybean peel; addition of corn grits and use of salt in the surface layer of the silo. The experimental design was completely randomized with six treatments and four replications. Plants of Tifton 85 bermudagrass with 38 days were ensiled in experimental silos with Bunsen valves, with compacting density of 236 kg silage per m³ for Tifton 85 bermudagrass, which was pre-dried in the sun; the average silage was 294 kg m-3 for the other treatments. The ratios between soybean hulls and corn grits, added to the silage, were calculated based on the initial DM content from Tifton 85 bermudagrass in order to obtain 320 g/kg DM of the material to be ensiled. There was an increase of lactic bacteria and Clostridium as well as an absence of enterobacteria after the silo opening. Fungi developed only in Tifton 85 bermudagrass and its treatments before the ensilage and yeasts developed in silages of Tifton 85 bermudagrass when they received soybean hulls or corn grits after silo opening. There was no breach regarding aerobic stability of silages during the studied period. The pH reached the highest values within 48 hours after opening the silos, but there were variations during this period.

The objective of this study was to estimate the dehydration curve and occurrence of fungi and mycotoxins in Tifton 85 hay. The experimental design was randomized blocks in split plots with four replications and five levels of nitrogen (0, 25, 50, 75 and 100 kg ha-1) in the form of urea, two regrowth ages (28 and 35 days) and drying hay in the sun and in shed. After harvesting, the grass was sampled four times (0, 8, 23 and 32 hours) to dry in the sun and twelve times (0, 3, 18, 27, 42, 51, 66, 75, 90, 99, 114 and 123 hours) for drying in shed, turned over daily. The fungi were identified after seeding and growth in three steps of haymaking (cutting, baling and after 30 days of storage). To verify the presence of mycotoxins, 20 samples, composed of hay stored for 30 days, were collected, of which 10 samples were from sun-dried hay and 10 from hay dried in shed. It took 32 hours to produce hay at field conditions, with water loss rates up to 6.10 g g-1 DM-h during the first 8 hours after cutting. The average loss of water in this period was 2.0 g g-1 DM-h. Hays dried in the shed took 123 hours and the dehydration rates were less than 0.5 g g-1 DM-h due to environmental conditions. There was a predominance of three genera of fungi: Fusarium, Penicillium and Aspergillus. The largest population was the Fusarium, followed by Penicillium, at the steps of baling and storage. The fumonisin mycotoxin was found at a higher concentration and there was no difference in fumonisin concentration in the hay dried in the sun and in shed. Although it brings no hazard to animal health, the production of aflatoxin and zearalenone was significantly higher in the samples of sun-dried hay.

The objective of this study was to evaluate the effect of fertilization with zinc or boron on the growth and dry matter production, nutritional value and accumulation of nutrients in white oats. The study comprised two experiments conducted in glasshouses, the first consisting of the application of four doses of zinc (0, 0.2, 0.4 and 0.6 mg/dm³) in the form of zinc sulphate (20% Zn), and the second consisting of the application of four doses of boron (0, 0.2, 0.4 and 0.6 mg/dm³) in the form of Borax (11% B). The experimental design in each case was a randomized block design, with five replicates. Fertilization with zinc and boron increased the growth of white oats, but had no significant effect on the nutritional value of the forage. Higher levels of absorption and accumulation of nutrients in plant tissues were observed following the application of boron and zinc at rates of up to 0.60 mg/dm³ of soil.

White oat and ryegrass were overseeded on Tifton 85 pastures for evaluation of dry matter yield (DMY), leaf:stem ratio (L:S), dehydration curves and nutritional value of forages. Dry matter intake and L:S ratio were analyzed using a completely randomized blocks design with five forage growth conditions (single Tifton 85 and intercropped with white oat or ryegrass, and white oat or ryegrass intercropped with Tifton 85), whereas for drying curves, ten dehydration times were tested (0, 4, 8, 24, 28, 32, 48, 52, 56 and 71 hours after harvest) by a 5 × 10 split-plot arrangement (growth conditions of forages as main plot and dehydration times as sub-plots). The nutritive value data were analyzed in a 3 × 3 split-plot trial with single Tifton 85, Tifton 85 - Oat and Tifton 85-Ryegrass as main plots and the three sampling times (before harvesting, at baling and 100 days after storage) as sub-plots. Oat and ryegrass showed higher L:S ratio, while the intercrops of Tifton 85 - Oat and Tifton 85 - Ryegrass presented lower DMY, but higher crude protein concentration and in vitro DM digestibility than single Tifton. Neutral and acid detergent fiber concentrations were higher for single Tifton 85. Ryegrass and white oat, overseeded on Tifton 85 pasture, improved the nutritional value of the hay produced; however, in a 100-day storage period, there was a reduction in in vitro dry matter digestibility and crude protein concentrations.

The study was conducted to estimate the dehydration curves, chemical composition, and occurrence of fungi in white oat hay (Avena sativa L. cv. Guapa BRS) and black oat hay (Avena strigosa Schreb cv. Common) at two cutting heights. Dehydration curves were studied under a randomized block design with a 2 × 2 factorial arrangement using split plots in time, considering two types of oats (white and black), two cutting heights (10 and 20 cm), and 17 sampling times (0, 4, 19, 24, 28, 43, 47, 52, 67, 71, 76, 91, 95, 100, 115, 129, and 124 hours after harvesting) with five replicates. For the chemical composition and occurrence of fungi, the experimental design comprised randomized blocks in a factorial 2 × 2 split-plot in time with two types of oats, two cutting heights, and three assessment periods: before cutting, during baling, and after 30 days of storage, with five replicates. The hay obtained by cutting of the black and white oats at heights of 10 and 20 cm showed similar dehydration curves. The crude protein values were higher in white oats only at the time of cutting (141.5 g/kg). The black oats showed lower nutritional quality, with higher levels of ADF and lignin. There was no effect of cutting height on the chemical composition, but the cutting height interfered with the production of dry matter and residue after cutting, with cutting at 10 cm leading to higher dry matter production and at 20 cm to increased waste production. The cutting heights of the oats interfere directly with the dry matter production and post-harvest residue without changing the chemical composition of the hay.

Objetivou-se avaliar características produtivas, estruturais e nutricionais dos capins Tifton 85 e Piatã e da leguminosa feijão-guandu em monocultivo ou em cultivo consorciado, com e sem aplicação de nitrogênio. Adotou-se o delineamento de blocos casualizados com parcelas subdivididas no tempo, com sete tratamentos nas parcelas principais e 3 tempos de avaliação nas sub-parcelas, com 4 repetições. Os tratamentos consistiram: Cajanus cajan cv. 'Super N' (feijão-guandu); Brachiaria brizantha cv. 'Piatã' solteira sem aplicação de nitrogênio (N); B. brizantha cv. 'Piatã' em associação com feijão guandu; B. brizantha cv. 'Piatã' solteira com aplicação de N (150kg ha-1); Cynodon sp. Tifton 85 em cultivo solteiro sem aplicação de N; Tifton 85 em associação com feijão-guandu e Tifton 85 com aplicação de N (150kg ha-1) e três períodos de avaliações. A associação dos capins Piatã e Tifton 85 com feijão-guandu proporcionou produção forrageira equivalente à adubação nitrogenada e incrementos com elevação nos teores de proteína bruta e redução nos teores de fibra em detergente neutro. O capim Tifton 85 se mostrou menos tolerante ao sombreamento imposto pelo feijão-guandu.

This trial aimed at evaluating characteristics as: production, structural and nutritional requirements of Tifton 85 and Piatã grasses and pigeonpea in single or intercropped tillage, with and without nitrogen application. The experimental plot in randomized blocks with plots subdivided through time, with 7 treatments in the main plots and three periods of evaluation on subplots with four replications. The studied treatments were: Cajanus cajan cv. Super N (pigeonpea), Brachiaria brizantha cv. single Piatã without application of nitrogen (N); B. brizantha cv. Piatã in association with pigeonpea; B. brizantha cv. single Piatã with application of N (150kg ha-1); Cynodon sp. Tifton 85 in a single crop without application of N; Tifton 85 in association with pigeonpea and Tifton 85 plus an application of N (150kg ha-1) and three cycles of evaluations. The association of Tifton 85 and Piatã grasses with pigeonpea provided forage production like nitrogen fertilizer and increase crude protein content and a reduction on neutral detergent fiber contents. Tifton grass was less tolerant to shading from pigeonpea.

The experiment was carried out to evaluate the structural characteristics, biomass accumulation, chemical composition and in vitro digestibility of dry matter and crude protein of white oat (Avena sativa L. IPR 126) under different management systems. Grazing, cut at two heights (15 and 20 cm) and free growth were all evaluated in three periods (July, August and September) with a 28-day average interval between evaluations. In the free growth system, samples at 15 and 20 cm were also taken at the intervals chosen for cutting and grazing. The experimental design was in randomized blocks with three replications, arranged in a 3 × 2 factorial split plot design over time; systems of management and heights were the factors of plots, and time was taken as subplot. There was higher straw production under free growth, but, with decreased chemical quality from the first to the second period and from the second to the third one, with 236.4, 172.5, and 91.4 g/kg crude protein values, respectively. Regarding cutting and grazing systems, they showed structural changes in tillering, which was favored by the cut, but with some reduction in the periods. Nutritional quality values were close to cutting and grazing with high crude protein content (216.6 g/kg), adequate neutral detergent fiber (535.4 g/kg) content and high in vitro digestibility of dry matter (826.3 g/kg). Management heights promoted few changes in the characteristics evaluated. After the third period, cutting and grazing systems showed no suitable residual straw for ground covering and set a summer crop under no-tillage system, with 738.39 kg/ha of residual dry matter on average.

This research study was conducted to evaluate, by means of microbiological analyses, the preservation of wet brewery waste stored under aerobic and anaerobic conditions, regarding the development of filamentous fungi, yeasts and lactic-acid bacteria. The following treatments were used: untreated brewery waste, pre-dried brewery waste silage, brewery waste silage with effluent drainage and BW silage without effluent drainage. Silos made of PVC and equipped with Bunsen valves to allow gases to escape were used. Dry matter (DM), crude protein (CP), temperature (°C) and pH in the untreated BW, in the stored brewery waste and in the brewery waste silage upon silo opening, after 60 days of ensilage were analyzed. A completely randomized design was used. The data were subjected to analysis of variance, and the means were compared by the Tukey test at the 5% probability level. The preservation of brewery waste packaged under aerobic conditions was not appropriate due to the development of filamentous fungi and yeasts; however, storage under anaerobic conditions proved to be an effective conservation process.

The objectives of this study were to estimate the curve of dehydration, bromatological composition and occurrence of fungi in Tifton 85 grass, submitted to four methods of dehydration. The curves of dehydration were studied in a completely randomized design with a 4 × 11 factorial scheme with four drying systems (1 conditioning + 1 turn-over; 2 conditionings + 1 turn-over; no conditioning + 1 turn-over and no conditioning + 2 turn-overs) and 11 sampling times (0, 3, 15, 18, 21, 24, 39, 42, 45 and 47 hours after the cutting), with six replicates. For the bromatological composition and fungi occurrence, the experimental design was completely randomized in a 4 × 3 factorial scheme with the four drying methods of Tifton 85 grass and three periods of evaluation (before the cutting, at the time of the baling and 30 days after storage) with six replicates. It was found that after the first hour after cutting and at the end of the period of dehydration, Tifton 85 plants that were treated with conditioners showed higher levels of dry matter. Stored hay that was treated with conditioners presented higher levels of acid detergent insoluble protein. Until baling, the drying methods did not alter the crude protein content of hay; however, after storage, the hay submitted only to turn-over showed crude protein content higher (13.90%) than those submitted to conditioners (9.94%). There was an increase in the occurrence of fungi after storage, but the application of conditioners and one turn-over resulted in lower CUF/g, compared with other treatments, enhancing the effect of conditioners on accelerating the dehydration rate and obtaining better hay storage conditions

The objective of this experiment was to estimate the curve of dehydration, chemical composition and in vitro dry matter digestibility (IVDMD) and crude protein digestibility (IVCPD) of tifton 85 hay, produced single or overseeded with ryegrass or white oat IPR 126. The experimental design was in randomized blocks, in a 3 × 10 factorial arrangement, with three cropping systems and ten evaluation times (0, 4, 8, 24, 28, 32, 48, 52 and 56 and 71 hours after harvest) during the dehydration process. For chemical composition and in vitro DM and CP digestibility, the experimental design was in randomized blocks with three cropping systems and three evaluation times (before cutting, before baling and 100 days after of storage). It was found that tifton 85 intercropped with white oat kept its higher nutritional value after storage, with 19.78% crude protein and 70.03% of in vitro dry matter digestibility. The participation of white oat in tifton 85 area was 57.04% and for ryegrass, it was 38.52%, but the dry matter yield of single tifton 85 was higher than other intercrops. Thus, it is recommended an oversowing of winter annual species on Tifton 85, without moisture restriction, because of the benefits obtained in the hay nutritional value.