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au:PINHEIRO, LUIZ GONZAGA PORTO

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Criopreserved ovarian tissue transplantation and bone restoration metabolism in castrated rats
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SILVA, LÍGIA HELENA FERREIRA MELO E

; SILVA, JOÃO MARCOS DE MENESES E

; SALAMA, MAHMOUD

; PINHEIRO, LUIZ GONZAGA PORTO

; LUNARDI, FRANCIELE OSMARINI

; SILVA, PAULO GOBERLÂNIO BARROS DA

; HIRTH, CARLOS GUSTAVO

; LUCENA, IGOR FREITAS DE

; GOMES, GLAUCO JORGE DA COSTA

; LEITE, JOSÉ ALBERTO DIAS

Revista do Colégio Brasileiro de Cirurgiões

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2018, Volume 45 N. 1 elocation e1577

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivos: avaliar os níveis de estradiol e os efeitos do autotransplante de tecido ovariano aquecido, após vitrificação, no metabolismo ósseo de ratas previamente ooforectomizadas bilateralmente. Métodos: trabalho experimental com 27 ratas com idades entre 11 e 12 semanas e pesando 200g a 300g, submetidas à ooforectomia bilateral e criopreservação de tecido ovariano para posterior reimplante. Os animais foram divididos em dois grupos, A e B, com oito e 19 ratas, respectivamente. O autotransplante ocorreu em dois períodos de acordo com o tempo de castração: após uma semana, no grupo A, e após um mês no grupo B. Mensurações de estradiol sérico e análise histológica de ovário e tíbia foram feitos antes e após o período de ooforectomia (precoce ou tardio) e um mês após o reimplante. Resultados: nos grupos A e B, as espessuras corticais médias da tíbia foram 0,463±0,14mm (média±DP) na linha de base, 0,360±0,14mm após ooforectomia e 0,445±0,17mm em um mês após o reimplante (p<0,005). As médias trabeculares foram 0,050±0,08mm (média±DP) na linha de base, 0,022±0,08mm após ooforectomia e 0,049±0,032mm em um mês após o reimplante (p<0,005). Não houve diferença estatística entre a variação do estradiol entre os dois grupos de estudo (p=0,819). Conclusão: o transplante de tecido ovariano criopreservado restabeleceu os parâmetros ósseos, e estes resultados sugerem que a reimplantação ovariana em mulheres pode apresentar os mesmos efeitos benéficos sobre o metabolismo ósseo.

ABSTRACT Objectives: to evaluate estradiol levels and autotransplantation heated ovarian tissue effects, after vitrification, on rats bone metabolism previously oophorectomized bilaterally. Methods: experimental study with 27 rats aged 11 to 12 weeks and weighing 200g to 300g, submitted to bilateral oophorectomy and ovarian tissue cryopreservation for subsequent reimplantation. Animals were divided into two groups, A and B, with 8 and 19 rats, respectively. Autotransplantation occurred in two periods according to castration time: after one week, in group A, and after one month in group B. Serum estradiol measurements and ovary and tibia histological analysis were performed before and after oophorectomy period (early or late) and one month after reimplantation. Results: in groups A and B, tibia median cortical thickness was 0.463±0.14mm (mean±SD) at the baseline, 0.360±0.14mm after oophorectomy and 0.445±0.17mm one month after reimplantation p<0.005). Trabecular means were 0.050±0.08mm (mean±SD) at baseline, 0.022±0.08mm after oophorectomy and 0.049±0.032mm one month after replantation (p<0.005). There was no statistical difference in estradiol variation between the two study groups (p=0.819). Conclusion: cryopreserved ovarian tissue transplantation restored bone parameters, and these results suggest that ovarian reimplantation in women may have the same beneficial effects on bone metabolism.

https://doi.org/10.1590/0100-6991e-20181577 2044 downloads

Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence
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Cavalcante, José Roosevelt

; Pinheiro, Luiz Gonzaga Porto

; Almeida, Paulo Roberto Carvalho de

; Ferreira, Márcia Valéria Pitombeira

; Cruz, Gizele Almada

; Campelo, Thales Alves

; Silva, Caroliny Soares

; Lima, Luana Nepomuceno Gondim Costa

; Oliveira, Bruno Masato Kitagawa de

; Lima, Lara Mulato

; Feitosa, Laura Magda Costa

; Pinheiro, Agostinho Câmara

; Frota, Cristiane Cunha

Clinics

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2018, Volume 73 elocation e465

Abstract: En | Text: En | PDF: En

OBJECTIVE: The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil. METHOD: One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant controls, were studied. DNA extraction was verified by human beta-globin gene amplification, and polymerase chain reaction was conducted based on HPV L1-specific consensus primers MY09/MY11 and GP5+/GP6+, followed by nested multiplex polymerase chain reaction with type-specific primers for the E6/E7 consensus region. RESULTS: Human papillomavirus DNA was detected in 51 (49.5%) breast carcinoma samples and 15 (15.8%) normal breast samples (p<0.0001). Human papillomavirus genotypes 6 and 11 were identified in 15.2% of all samples. CONCLUSIONS: The high frequency of human papillomavirus infection in breast cancer samples indicates a potential role of this virus in breast carcinogenesis in the studied participants.

https://doi.org/10.6061/clinics/2018/e465 897 downloads

Identification of the sentinel lymph node using hemosiderin in locally advanced breast cancer
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DE AGUIAR, PAULO HENRIQUE WALTER

; AQUINO, RANNIERE GURGEL FURTADO DE

; ALVES, MAYARA MAIA

; CORREIA, JULIO MARCUS SOUSA

; OLIVEIRA, AYANE LAYNE DE SOUSA

; VIANA JÚNIOR, ANTÔNIO BRAZIL

; PINHEIRO, LUIZ GONZAGA PORTO

Revista do Colégio Brasileiro de Cirurgiões

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dec 2017, Volume 44 N. 6 Pages 612 - 618

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: verificar a taxa de concordância na identificação do linfonodo sentinela utilizando um marcador autólogo rico em hemossiderina e o Tecnécio 99 (Tc99) em casos de câncer de mama localmente avançados. Métodos: ensaio clínico fase 1, do tipo prospectivo, não randomizado, em 18 pacientes portadoras de câncer de mama com axila clinicamente negativa em estádio T2=4cm, T3 e T4. As pacientes foram submetidas à injeção subareolar de um marcador autólogo rico em hemossiderina 48 horas antes do procedimento cirúrgico para biópsia do linfonodo sentinela, e sua taxa de identificação foi comparada, no intraoperatório, com o marcador radioativo Tc99 (padrão-ouro). A concordância entre os métodos foi estabelecida pelo índice de Kappa. Resultados: a taxa de identificação do linfonodo sentinela foi de 88,9%, com uma média de dois linfonodos sentinelas por paciente. O estudo identificou os linfonodos sentinelas corados com hemossiderina em 83,3% dos casos (n=15), quando comparados com a taxa de identificação do Tc99, tendo sido observada concordância em 94,4% dos casos estudados. Conclusão: o marcador autólogo rico em hemossiderina se mostrou eficaz na identificação do linfonodo sentinela em casos de câncer de mama localmente avançado.

ABSTRACT Objective: to verify the agreement rate in the identification of sentinel lymph node using an autologous marker rich in hemosiderin and 99 Technetium (Tc99) in patients with locally advanced breast cancer. Methods: clinical trial phase 1, prospective, non-randomized, of 18 patients with breast cancer and clinically negative axilla stages T2=4cm, T3 and T4. Patients were submitted to sub-areolar injection of hemosiderin 48 hours prior to sentinel biopsy surgery, and the identification rate was compared at intraoperative period to the gold standard marker Tc99. Agreement between methods was determined by Kappa index. Results: identification rate of sentinel lymph node was 88.9%, with a medium of two sentinel lymph nodes per patients. The study identified sentinel lymph nodes stained by hemosiderin in 83.3% patients (n=15), and, compared to Tc99 identification, the agreement rate was 94.4%. Conclusion: autologous marker rich in hemosiderin was effective to identify sentinel lymph nodes in locally advanced breast cancer patients.

https://doi.org/10.1590/0100-69912017006013 3075 downloads

Invasive ductal carcinoma: relationship between pathological characteristics and the presence of axillary metastasis in 220 cases
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AQUINO, RANNIERE GURGEL FURTADO DE

; VASQUES, PAULO HENRIQUE DIÓGENES

; CAVALCANTE, DIANE ISABELLE MAGNO

; OLIVEIRA, AYANE LAYNE DE SOUSA

; OLIVEIRA, BRUNO MASATO KITAGAWA DE

; PINHEIRO, LUIZ GONZAGA PORTO

Revista do Colégio Brasileiro de Cirurgiões

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apr 2017, Volume 44 N. 2 Pages 163 - 170

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: analisar a relação das características anatomopatológicas com o comprometimento axilar em casos de carcinoma ductal invasor. Métodos: estudo transversal de 220 pacientes com câncer de mama, submetidas à mastectomia radical ou quadrantectomia com esvaziamento axilar, oriundos do Serviço de Mastologia da Maternidade Escola Assis Chateaubriand, Ceará, Brasil. Os tumores foram submetidos a processamento histológico e, em seguida, foram determinados os graus histológico (GH), tubular (GT), nuclear (GN), índice mitótico (IM) pela classificação de Scarff-Bloom-Richadson, verificada a presença de invasão angiolinfática (IA) e mensurado o maior diâmetro do tumor (DT). Tais variáveis foram correlacionadas com a presença de metástases axilares. Resultados: a média de idade das pacientes foi 56,81 anos ± 13,28. O tamanho do tumor variou de 0,13 a 22 cm, com média de 2,23cm ± 2,79. Os GH3, GT3 e GN3 prevaleceram: n=107 (48,6%), n=160 (72,7%) e n=107 (48,6%), respectivamente. Os índices mitóticos 1, 2 e 3 apresentaram distribuição homogênea: n=82 (37,2%), n=68 (31%) e n=70 (31,8%), respectivamente. Não foi evidenciada relação do GH, GT e GN com a ocorrência de metástases axilares (p=0,07; p=0,22 e p=0,21). Índices mitóticos 2 e 3 apresentaram relação com a o ocorrência de metástases axilares (p=0,03). Tumores maiores do que 2cm e casos com invasão angiolinfática apresentaram maior índice de metástases axilares (p=0,0003 e p<0,0001). Conclusão: índices mitóticos elevados, tumores com diâmetro maior do que 2cm e presença de invasão angiolinfática apresentaram isoladamente relação com a ocorrência de metástases axilares.

ABSTRACT Objective: to analyze the relation of anatomopathological features and axillary involvement in cases of invasive ductal carcinoma. Methods: this is a cross-sectional study of 220 breast cancer patients submitted to radical mastectomy or quadrantectomy with axilar emptying, from the Mastology Service of the Assis Chateaubriand Maternity School, Ceará, Brazil. We submitted the tumors to histological processing and determined the histological (HG), tubular (TG) and nuclear (NG) grades, and the mitotic index (MI) by the classification of Scarff-Bloom-Richadson, verified the presence of angiolymphatic invasion (AI) and measured the largest tumor diameter (TD). We then correlated these variables with the presence of axillary metastases. Results: the mean patients'age was 56.81 years ± 13.28. Tumor size ranged from 0.13 to 22 cm, with an average of 2.23cm ± 2.79. HG3, TG3 and NG3 prevailed, respectively 107 (48.6%), 160 (72.7%) and 107 (48.6%). Mitotic indexes 1, 2 and 3 presented a homogeneous distribution, respectively 82 (37.2%), 68 (31%) and 70 (31.8%). We observed no relation between the HG, TG and NG with the occurrence of axillary metastases (p=0.07, p=0.22 and p=0.21, respectively). Mitotic indices 2 and 3 were related with the occurrence of axillary metastases (p=0.03). Tumors larger than 2cm and cases that presented angiolymphatic invasion had a higher index of axillary metastases (p=0.0003 and p<0.0001). Conclusion: elevated mitotic indexes, tumors with a diameter greater than 2cm and the presence of angiolymphatic invasion were individuallyassociatedwith the occurrence of axillary metastases.

https://doi.org/10.1590/0100-69912017002010 38860 downloads

ERICA: prevalence of metabolic syndrome in Brazilian adolescents
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Kuschnir, Maria Cristina C

; Bloch, Katia Vergetti

; Szklo, Moyses

; Klein, Carlos Henrique

; Barufaldi, Laura Augusta

; Abreu, Gabriela de Azevedo

; Schaan, Beatriz

; Veiga, Gloria Valeria da

; Silva, Thiago Luiz Nogueira da

; Vasconcellos, Maurício T L de

; Moraes, Ana Júlia Pantoja de

; Oliveira, Ana Mayra Andrade de

; Tavares, Bruno Mendes

; Oliveira, Cecília Lacroix de

; Cunha, Cristiane de Freitas

; Giannini, Denise Tavares

; Belfort, Dilson Rodrigues

; Santos, Eduardo Lima

; Leon, Elisa Brosina de

; Oliveira, Elizabete Regina Araújo

; Fujimori, Elizabeth

; Borges, Ana Luíza

; Magliano, Erika da Silva

; Vasconcelos, Francisco de Assis Guedes

; Azevedo, George Dantas

; Brunken, Gisela Soares

; Guimarães, Isabel Cristina Britto

; Faria Neto, José Rocha

; Oliveira, Juliana Souza

; Carvalho, Kenia Mara B de

; Gonçalves, Luis Gonzaga de Oliveira

; Monteiro, Maria Inês

; Santos, Marize M

; Muniz, Pascoal Torres

; Jardim, Paulo César B Veiga

; Ferreira, Pedro Antônio Muniz

; Montenegro Jr, Renan Magalhães

; Gurgel, Ricardo Queiroz

; Vianna, Rodrigo Pinheiro

; Vasconcelos, Sandra Mary

; Martins, Stella Maris Seixas

; Goldberg, Tamara Beres Lederer

Revista de Saúde Pública

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2016, Volume 50 Suppl. 1 elocation 11s

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO OBJETIVO Determinar a prevalência de síndrome metabólica e de seus componentes em adolescentes brasileiros. MÉTODOS Foram avaliados 37.504 adolescentes, participantes do Estudo de Riscos Cardiovasculares em Adolescentes (ERICA), estudo transversal de âmbito nacional e de base escolar. Os adolescentes, de 12 a 17 anos de idade, residiam em municípios com mais de 100 mil habitantes. A amostra foi estratificada e conglomerada em escolas e turmas. Os critérios da International Diabetes Federation foram utilizados para definir síndrome metabólica. Prevalências de síndrome metabólica foram estimadas segundo sexo, faixa etária, tipo de escola e estado nutricional. RESULTADOS Dos 37.504 adolescentes, 50,2% eram do sexo feminino; 54,3% tinham de 15 a 17 anos e 73,3% estudavam em escolas públicas. A prevalência nacional de síndrome metabólica foi 2,6% (IC95% 2,3-2,9), discretamente maior no sexo masculino e naqueles de 15 a 17 anos na maioria das macrorregiões. A prevalência foi a maior nos residentes na macrorregião Sul, nas adolescentes mais jovens e nos adolescentes mais velhos. A prevalência foi maior nas escolas públicas (2,8% [IC95% 2,4-3,2]) que nas escolas privadas (1,9% [IC95% 1,4-2,4]) e nos adolescentes obesos em comparação aos não obesos. As combinações de componentes mais frequentes, respondendo por 3/4 das combinações, foram: circunferência de cintura elevada, HDL-colesterol baixo e pressão arterial elevada, seguida de circunferência da cintura elevada, lipoproteína de alta densidade (HDL-c) baixo e triglicerídeos elevados, e wpor circunferência da cintura elevada, HDL baixa e triacilgliceróis e pressão arterial elevados. A HDL baixa foi o segundo componente mais frequente, mas a maior prevalência de síndrome metabólica (26,8%) foi observada na presença de triglicerídeos elevado. CONCLUSÕES O ERICA é o primeiro estudo nacional a apresentar prevalências de síndrome metabólica e descrever a participação dos seus componentes. Apesar de a prevalência da síndrome metabólica ter sido baixa, as altas prevalências de alguns componentes e de participação de outros na composição da síndrome torna importante o diagnóstico precoce de tais alterações, mesmo que não agrupadas na síndrome metabólica.

ABSTRACT OBJECTIVE To determine the prevalence of metabolic syndrome and its components in Brazilian adolescents. METHODS We evaluated 37,504 adolescents who were participants in the Study of Cardiovascular Risks in Adolescents (ERICA), a cross-sectional, school-based, national study. The adolescents, aged from 12 to 17 years, lived in cities with populations greater than 100,000 inhabitants. The sample was stratified and clustered into schools and classes. The criteria set out by the International Diabetes Federation were used to define metabolic syndrome. Prevalences of metabolic syndrome were estimated according to sex, age group, school type and nutritional status. RESULTS Of the 37,504 adolescents who were evaluated: 50.2% were female; 54.3% were aged from 15 to 17 years, and 73.3% were from public schools. The prevalence of metabolic syndrome was 2.6% (95%CI 2.3-2.9), slightly higher in males and in those aged from 15 to 17 years in most macro-regions. The prevalence was the highest in residents from the South macro-region, in the younger female adolescents and in the older male adolescents. The prevalence was higher in public schools (2.8% [95%CI 2.4-3.2]), when compared with private schools (1.9% [95%CI 1.4-2.4]) and higher in obese adolescents when compared with nonobese ones. The most common combinations of components, referring to 3/4 of combinations, were: enlarged waist circumference (WC), low HDL-cholesterol (HDL-c) and high blood pressure; followed by enlarged WC, low HDL-c and high triglycerides; and enlarged WC, low HDL-c, high triglycerides and blood pressure. Low HDL was the second most frequent component, but the highest prevalence of metabolic syndrome (26.8%) was observed in the presence of high triglycerides. CONCLUSIONS ERICA is the first Brazilian nation-wide study to present the prevalence of metabolic syndrome and describe the role of its components. Despite the prevalence of Metabolic Syndrome being low, the high prevalences of some components and participation of others in the syndrome composition shows the importance of early diagnosis of this changes, even if not grouped within the metabolic syndrome.

https://doi.org/10.1590/s01518-8787.2016050006701 1789 downloads

ERICA: prevalences of hypertension and obesity in Brazilian adolescents
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Bloch, Katia Vergetti

; Klein, Carlos Henrique

; Szklo, Moyses

; Kuschnir, Maria Cristina C

; Abreu, Gabriela de Azevedo

; Barufaldi, Laura Augusta

; Veiga, Gloria Valeria da

; Schaan, Beatriz

; Silva, Thiago Luiz Nogueira da

; Moraes, Ana Julia Pantoja

; Oliveira, Ana Mayra Andrade de

; Tavares, Bruno Mendes

; Magliano, Erika da Silva

; Oliveira, Cecília Lacroix de

; Cunha, Cristiane de Freitas

; Giannini, Denise Tavares

; Belfort, Dilson Rodrigues

; Santos, Eduardo Lima

; Leon, Elisa Brosina de

; Oliveira, Elizabete Regina Araújo

; Fujimori, Elizabeth

; Borges, Ana Luíza

; Vasconcelos, Francisco de Assis Guedes

; Azevedo, George Dantas

; Brunken, Gisela Soares

; Guimarães, Isabel Cristina Britto

; Faria Neto, José Rocha

; Oliveira, Juliana Souza

; Carvalho, Kenia Mara B. de

; Gonçalves, Luis Gonzaga de Oliveira

; Monteiro, Maria Inês

; Santos, Marize M.

; Jardim, Paulo César B. Veiga

; Ferreira, Pedro Antônio Muniz

; Montenegro Jr., Renan Magalhães

; Gurgel, Ricardo Queiroz

; Vianna, Rodrigo Pinheiro

; Vasconcelos, Sandra Mary

; Goldberg, Tamara Beres Lederer

Revista de Saúde Pública

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2016, Volume 50 Suppl. 1 elocation 9s

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO OBJETIVO Estimar as prevalências de hipertensão arterial e obesidade e a fração atribuível populacional de hipertensão arterial devida à obesidade em adolescentes brasileiros. MÉTODOS Foram avaliados dados dos participantes do Estudo de Riscos Cardiovasculares em Adolescentes, estudo seccional l nacional de base escolar. A amostra foi dividida em 32 estratos geográficos e conglomerados de escolas e turmas, com representatividade nacional, macrorregional e de capitais. Obesidade foi classificada pelo índice de massa corporal segundo idade e sexo. Considerou-se hipertensão arterial a média da pressão arterial sistólica ou diastólica maior ou igual ao percentil 95 da curva de referência. Foram estimadas prevalências e intervalos de confiança de 95% (IC95%) de hipertensão arterial e de obesidade, nacionais e nas macrorregiões do País, por sexo e grupo etário, assim como as frações de hipertensão atribuíveis à obesidade na população. RESULTADOS Foram avaliados 73.399 estudantes, 55,4% do sexo feminino, com média de idade 14,7 anos (DP = 1,6). A prevalência de hipertensão arterial foi 9,6% (IC95% 9,0-10,3); sendo as mais baixas observadas nas regiões Norte, 8,4% (IC95% 7,7-9,2) e Nordeste, 8,4% (IC95% 7,6-9,2) e a mais alta na região Sul, 12,5% (IC95% 11,0-14,2). A prevalência de obesidade foi 8,4% (IC95% 7,9-8,9), mais baixa na região Norte e mais alta na Sul. As prevalências de hipertensão arterial e obesidade foram maiores no sexo masculino. Adolescentes com obesidade tiveram prevalência de hipertensão arterial mais elevada, 28,4% (IC95% 25,5-31,2), do que aqueles com sobrepeso, 15,4% (IC95% 13,8-17,0), ou eutróficos, 6,3% (IC95% 5,6-7,0). A fração de hipertensão arterial atribuível à obesidade foi de 17,8%. CONCLUSÕES O ERICA foi o primeiro estudo brasileiro com representatividade nacional a estimar a prevalência de hipertensão arterial aferida em adolescentes. A fração da prevalência de hipertensão arterial atribuível à obesidade mostrou que cerca de 1/5 dos hipertensos poderiam não ser hipertensos se não fossem obesos.

ABSTRACT OBJECTIVE To estimate the prevalence of arterial hypertension and obesity and the population attributable fraction of hypertension that is due to obesity in Brazilian adolescents. METHODS Data from participants in the Brazilian Study of Cardiovascular Risks in Adolescents (ERICA), which was the first national school-based, cross-section study performed in Brazil were evaluated. The sample was divided into 32 geographical strata and clusters from 32 schools and classes, with regional and national representation. Obesity was classified using the body mass index according to age and sex. Arterial hypertension was defined when the average systolic or diastolic blood pressure was greater than or equal to the 95th percentile of the reference curve. Prevalences and 95% confidence intervals (95%CI) of arterial hypertension and obesity, both on a national basis and in the macro-regions of Brazil, were estimated by sex and age group, as were the fractions of hypertension attributable to obesity in the population. RESULTS We evaluated 73,399 students, 55.4% female, with an average age of 14.7 years (SD = 1.6). The prevalence of hypertension was 9.6% (95%CI 9.0-10.3); with the lowest being in the North, 8.4% (95%CI 7.7-9.2) and Northeast regions, 8.4% (95%CI 7.6-9.2), and the highest being in the South, 12.5% (95%CI 11.0-14.2). The prevalence of obesity was 8.4% (95%CI 7.9-8.9), which was lower in the North region and higher in the South region. The prevalences of arterial hypertension and obesity were higher in males. Obese adolescents presented a higher prevalence of hypertension, 28.4% (95%CI 25.5-31.2), than overweight adolescents, 15.4% (95%CI 17.0-13.8), or eutrophic adolescents, 6.3% (95%CI 5.6-7.0). The fraction of hypertension attributable to obesity was 17.8%. CONCLUSIONS ERICA was the first nationally representative Brazilian study providing prevalence estimates of hypertension in adolescents. Regional and sex differences were observed. The study indicates that the control of obesity would lower the prevalence of hypertension among Brazilian adolescents by 1/5.

https://doi.org/10.1590/s01518-8787.2016050006685 1511 downloads

ERICA: prevalences of hypertension and obesity in Brazilian adolescents
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Bloch, Katia Vergetti

; Klein, Carlos Henrique

; Szklo, Moyses

; Kuschnir, Maria Cristina C

; Abreu, Gabriela de Azevedo

; Barufaldi, Laura Augusta

; Veiga, Gloria Valeria da

; Schaan, Beatriz

; Silva, Thiago Luiz Nogueira da

; Moraes, Ana Julia Pantoja

; Oliveira, Ana Mayra Andrade de

; Tavares, Bruno Mendes

; Magliano, Erika da Silva

; Oliveira, Cecília Lacroix de

; Cunha, Cristiane de Freitas

; Giannini, Denise Tavares

; Belfort, Dilson Rodrigues

; Santos, Eduardo Lima

; Leon, Elisa Brosina de

; Oliveira, Elizabete Regina Araújo

; Fujimori, Elizabeth

; Borges, Ana Luíza

; Vasconcelos, Francisco de Assis Guedes

; Azevedo, George Dantas

; Brunken, Gisela Soares

; Guimarães, Isabel Cristina Britto

; Faria Neto, José Rocha

; Oliveira, Juliana Souza

; Carvalho, Kenia Mara B. de

; Gonçalves, Luis Gonzaga de Oliveira

; Monteiro, Maria Inês

; Santos, Marize M.

; Jardim, Paulo César B. Veiga

; Ferreira, Pedro Antônio Muniz

; Montenegro Jr., Renan Magalhães

; Gurgel, Ricardo Queiroz

; Vianna, Rodrigo Pinheiro

; Vasconcelos, Sandra Mary

; Goldberg, Tamara Beres Lederer

Revista de Saúde Pública

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2016, Volume 50 Suppl. 1 elocation 9s

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/s01518-8787.2016050006685 18629 downloads

ERICA: prevalence of metabolic syndrome in Brazilian adolescents
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Kuschnir, Maria Cristina C

; Bloch, Katia Vergetti

; Szklo, Moyses

; Klein, Carlos Henrique

; Barufaldi, Laura Augusta

; Abreu, Gabriela de Azevedo

; Schaan, Beatriz

; Veiga, Gloria Valeria da

; Silva, Thiago Luiz Nogueira da

; Vasconcellos, Maurício T L de

; Moraes, Ana Júlia Pantoja de

; Oliveira, Ana Mayra Andrade de

; Tavares, Bruno Mendes

; Oliveira, Cecília Lacroix de

; Cunha, Cristiane de Freitas

; Giannini, Denise Tavares

; Belfort, Dilson Rodrigues

; Santos, Eduardo Lima

; Leon, Elisa Brosina de

; Oliveira, Elizabete Regina Araújo

; Fujimori, Elizabeth

; Borges, Ana Luíza

; Magliano, Erika da Silva

; Vasconcelos, Francisco de Assis Guedes

; Azevedo, George Dantas

; Brunken, Gisela Soares

; Guimarães, Isabel Cristina Britto

; Faria Neto, José Rocha

; Oliveira, Juliana Souza

; Carvalho, Kenia Mara B de

; Gonçalves, Luis Gonzaga de Oliveira

; Monteiro, Maria Inês

; Santos, Marize M

; Muniz, Pascoal Torres

; Jardim, Paulo César B Veiga

; Ferreira, Pedro Antônio Muniz

; Montenegro Jr, Renan Magalhães

; Gurgel, Ricardo Queiroz

; Vianna, Rodrigo Pinheiro

; Vasconcelos, Sandra Mary

; Martins, Stella Maris Seixas

; Goldberg, Tamara Beres Lederer

Revista de Saúde Pública

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2016, Volume 50 Suppl. 1 elocation 11s

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/s01518-8787.2016050006701 11875 downloads

Comparison between hemosiderin and Technetium-99 in sentinel lymph node biopsy in human breast cancer
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Vasques, Paulo Henrique Diógenes

; Alves, Mayara Maia

; Aquino, Ranniere Gurgel Furtado de

; Torres, Roberto Vitor Almeida

; Bezerra, José Lucas Martins

; Brasileiro, Luis Porto

; Pinheiro, Luiz Gonzaga Porto

Acta Cirúrgica Brasileira

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nov 2015, Volume 30 N. 11 Pages 785 - 790

Abstract: En | Text: En | PDF: En

PURPOSE: To assess the safety and potential equivalence of the use of hemosiderin compared to the Technetium-99 in sentinel lymph node biopsy in human breast cancer. METHODS: Non-random sample of 14 volunteer women diagnosed with breast cancer with primary tumors (T1/T2) and clinically tumor-free axilla were submitted to the identification of sentinel lymph node using hemosiderin obtained from autologous blood injected in the periareolar region 24h before surgery on an outpatient basis. Patients received preoperative subareolar intradermal injection of Technetium-99 in the immediate preoperative period. Patients were submitted to sentinel lymph node biopsy, with incision in the axillary fold guided by Gamma-Probe, dissection by planes until the identification of the point of maximum uptake of Technetium-99, identifying the marked nodes and their colors. All surgical specimens were sent for pathological and immunohistochemical study. RESULTS: The results showed no evidence of side effects and/or allergic and non-allergic reactions in patients submitted to SLNB with hemosiderin. The SLN identification rate per patient was 100%. SLNB identification rate per patient with hemosiderin was the same as that of Technetium, with a concordance rate of 100% between the methods. CONCLUSION: Hemosiderin is a safe dye that is equivalent to Technetium in breast sentinel lymph node biopsy.

https://doi.org/10.1590/S0102-865020150110000010 2266 downloads

10.

Histological study of rat ovaries cryopreserved by vitrification or slow freezing and reimplanted in the early or late postmenopausal stage
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Silva, João Marcos de Meneses e

; Pinheiro, Luiz Gonzaga Porto

; Leite, José Alberto Dias

; Melo, Lígia Helena Ferreira

; Lunardi, Franciele Osmarini

; Barbosa Filho, Rômulo Cesar Costa

; Mendonça, Cindy Vitalino

Acta Cirúrgica Brasileira

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may 2014, Volume 29 N. 5 Pages 299 - 305

Abstract: En | Text: En | PDF: En

PURPOSE: To compare two rat ovary cryopreservation techniques (vitrification vs. slow freezing) and two postmenopausal stages (early vs. late) with regard to graft take.METHODS:Thirty-three Wistar rats were submitted to bilateral oophorectomy. One ovary was submitted to histological analysis while the other was cryopreserved by slow freezing or vitrification. The cryopreserved ovary was thawed and reimplanted in the greater omentum one week (early menopause) or one month (late menopause) after oophorectomy. One month after ovary reimplantation, the graft take was evaluated macroscopically and histologically.RESULTS: Six of the animals were used ascontrols and seven died. The histological findings of 20 animals included atretic follicles (n=4), primordial follicles (n=2), and corpus luteum with primordial follicles (n=3). No ovarian tissue was found in 11 animals. Vitrification resulted in a higher graft take rate than slow freezing (50% vs. 38.5%), but the difference was not statistically significant. However, the graft take rate was 9.3 times higher in the early than in the late postmenopausal stage (61.5% vs. 14.3%) (p=0.043).CONCLUSION: Vitrification was superior to slow freezing as ovarian cryopreservation technique, and grafting was significantly more successful when the ovary was reimplanted in the late postmenopausal stage.

https://doi.org/10.1590/S0102-86502014000500003 4073 downloads

11.

Sentinel lymph node biopsy in patients with locally advanced breast cancer after neoadjuvant chemotherapy
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Aguiar, Paulo Henrique Walter

; Pinheiro, Luiz Gonzaga Porto

; Mota, Rosa Maria Salani

; Margotti, Nair Hermínia Gurjão

; Rocha, João Ivo Xavier

Acta Cirúrgica Brasileira

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dec 2012, Volume 27 N. 12 Pages 912 - 916

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Verificar a taxa de identificação do linfonodo sentinela (LS) em pacientes portadoras de câncer na mama localmente avançado e que foram submetidos à quimioterapia neoadjuvante comparando a citologia de contato intraoperatória (imprint) e a inclusão em parafina e validação dos procedimentos. MÉTODOS: Estudo transversal de validação de teste diagnóstico envolvendo 34 pacientes oriundas do Ambulatório da Maternidade-Escola Assis Chateaubriand. As pacientes eram portadoras de câncer na mama localmente avançado e foram tratadas com quimioterapia neoadjuvante. Àquelas com axila clinicamente negativa foram submetidas à biopsia do LS, esse estudado por impint e histopatologia em parafina. Todas as pacientes foram submetidas a esvaziamento axilar e seu estudo histopatológico. RESULTADOS: A taxa de identificação do LS foi de 85,3% (29/34). A sensibilidade do imprint associada à parafina quanto à detecção de metástase comparado a análise histopatológica do conteúdo axilar foi de 84,62% e a especificidade de 100% com taxa de falso-negativo de 12,01% e acurácia de 92,77%. CONCLUSÃO: A pesquisa de metástases no linfonodo sentinela por imprint e análise histopatológica em parafina quando comparado ao padrão ouro (esvaziamento axilar) apresentou baixa sensibilidade com taxa elevada de falsos negativos em nossa amostra.

PURPOSE: To check the rate of sentinel lymph node (SLN) identification in patients with locally advanced breast cancer who underwent neoadjuvant chemotherapy comparing intraoperative contact cytology (imprint) and embedded in paraffin and validation of methods. METHODS: A cross-sectional validation of diagnostic test involving 34 patients from the outpatient clinic of the Maternity School Assis Chateaubriand. The patients had locally advanced breast cancer and were treated with neoadjuvant chemotherapy. Those with clinically negative axilla underwent SLN biopsy, studied by imprint and histopathology in paraffin. All patients underwent axillary dissection and its histopathological study. RESULTS: The SLN identification rate was 85.3% (29/34). The sensitivity of imprint associated with paraffin on detection of metastasis compared to histopathology of the axillary content was 84.62% and specificity of 100% with false-negative rate of 12.01% and an accuracy of 92.77%. CONCLUSION: The search for metastases in the SLN by imprint and histopathological analysis in paraffin compared to the gold standard (axillary dissection) had a low sensitivity with high rate of false negatives in our sample.

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12.

Consumo alimentar de mulheres sobreviventes de câncer de mama: análise em dois períodos de tempo
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Sampaio, Helena Alves de Carvalho

; Rocha, Daianne Cristina

; Sabry, Maria Olganê Dantas

; Pinheiro, Luiz Gonzaga Porto

Revista de Nutrição

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oct 2012, Volume 25 N. 5 Pages 594 - 606

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Comparar o consumo alimentar de dois grupos de mulheres portadoras de câncer de mama atendidas em dois períodos distintos: 1999-2004 e 2005-2009. MÉTODOS: Estudo documental, com abordagem descritiva e comparativa. Foram coletados dados de identificação e de ingestão alimentar nas fichas de 154 pacientes portadoras de câncer de mama atendidas em serviços de referência de Fortaleza (CE): 66 no período de 1999-2004 e 88 no de 2005-2009. Foram considerados como integrantes do hábito os alimentos consumidos pelo menos quinzenalmente por, no mínimo, 50% das pessoas. Calculou-se a prevalência de consumo de alimentos, sendo os padrões dos dois períodos comparados pela aplicação dos testes Quiquadrado ou exato de Fisher, e adotouse p<0,05 como nível de significância. RESULTADOS: Os hábitos alimentares dos dois grupos foram semelhantes, exceto quanto ao maior consumo de leite e de cenoura e menor consumo de manga e caju, além de uma tendência ao aumento no consumo de óleo de soja, observada no grupo atendido mais recentemente. O aumento de leite e de cenoura pode configurar proteção contra a recidiva da doença, mas a redução do consumo das frutas citadas pode significar maior risco: aliás, foram baixas as prevalências de consumo de frutas e hortaliças pelos dois grupos de mulheres. CONCLUSÃO: Os achados apontam para uma lacuna na abordagem educativa das pacientes tanto no passado como na atualidade, o que comprova a necessidade de incentivar maior consumo e variedade de hortaliças e frutas e menor consumo de cereais refinados, gorduras, açúcar e refrigerantes.

OBJECTIVE: The aim of this study is to compare the dietary intake of two groups of women with breast cancer treated on different periods: 1999-2004 and 2005-2009. METHODS: This descriptive and comparative documental study collected data from the medical records of 154 patients with breast cancer treated in reference centers of Fortaleza (CE), Brazil. The following variables were studied: food intake and characterization. Sixtysix women were studied from 1999-2004 and 88 from 2005-2009. Foods consumed at least every 15 days by not less than 50% of the women were considered part of their eating habits. The prevalence of the foods consumed was calculated, and the standards of the two periods compared by the Chisquare or Fisher's exact test. The significance level was set at 5% (p<0.05). RESULTS: The dietary habits of both groups were similar but the group treated more recently consumed more milk, less carrots, mangos and cashews, and tended to consume more soybean oil. Higher intakes of milk and carrots can provide protection against recurrence, but lower consumption of fruits may increase risk. CONCLUSION: These findings indicate that patients are not being properly advised, both in the past and today. Patients need to be encouraged to consume more vegetables and fruits in volume and variety, and less refined grains, fats, sugar and soda.

12239 downloads

13.

Experimental canine model for sentinel lymph node biopsy in the vulva using technetium and patent blue dye
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Aquino, José Ulcijara

; Pinheiro, Luiz Gonzaga Porto

; Vasques, Paulo Henrique Diógenes

; Rocha, João Ivo Xavier

; Cruz, Diego Alves

; Beserra, Hugo Enrique Orsini

; Cavalcante, Raissa Vasconcelos

Acta Cirúrgica Brasileira

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feb 2012, Volume 27 N. 2 Pages 102 - 108

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: Definir um modelo experimental para a pesquisa do linfonodo sentinela na vulva de cadelas. MÉTODOS: 0,2 ml de tecnécio diluído em fitato (99mTc) foi injetado por via intradérmica na comissura anterior da vulva. Trinta minutos após a injeção de 99mTc, o mapeamento inguinal foi realizada utilizando uma sonda gama. Após isto, 0,5 ml de corante azul (bleu Patente Guerbet V 2,5%) foram injetados no mesmo lugar. Após 15 minutos, uma incisão inguinal de três centímetros foi feita no ponto de captação máxima seguido de dissecção cuidadosa, guiada através da visualização de um sistema linfático aferente corado em azul que apontava para o linfonodo sentinela (LS). RESULTADOS: Foi observado que em 88% das vezes o LS foi identificado. Não foi encontrada diferença significativa entre a presença ou não de linfonodo sentinela nos lados direito e esquerdo, o que é uma indicação de uma boa consistência do método. Foi observada uma alta (88%) e significativa (χ2 = 12,89 e p = 0,0003) intercessão entre os dois métodos (corante azul e radiação). CONCLUSÃO: O modelo experimental adotado é viável, tornando-se vantajoso na aplicação da associação de Azul Patente e 99mTc.

PURPOSE: This paper aims to study and define the experimental model of sentinel lymph node biopsy of the vulva in bitches. METHODS: 0.2 ml of 99mTc phytate was injected intradermally, using a fine gauge insulin needle in the anterior commissure of the vulva. Thirty minutes after 99mTc injection, the inguinal mapping was performed using a gamma probe. After this, 0.5 ml of blue dye (bleu patenté V Guerbet 2.5%) was injected in the same place. After 15 minutes, a 3 cm long inguinal incision was made at point maximum uptake followed by careful dissection, guided by visualization of a bluish afferent lymphatic system that points to the sentinel lymph node (SLN). RESULTS: It was observed that 88% of SLN were identified. It wasn´t found a significant difference among the presence or not of sentinel lymph node in the sides, which is an indication of a good consistency. It was observed a high (88%) and significant (χ2=12.89 and p=0.0003) intercession between both methods (blue dye and radiation). CONCLUSION: The experimental model adopted is feasible, becoming advantageous in applying the association of Patent blue and 99mTc.

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14.

Uso do retalho miogorduroso segmentar do músculo latíssimo do dorso em decúbito único para reconstrução da mama pós-quadrantectomia externa
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Araújo, João Batista Fortaleza de

; Pinheiro, Luiz Gonzaga Porto

; Sousa, Sérgio Saboya Pinheiro de

; Rocha, João Ivo Xavier

; Gaspar, Patrícia Lopes

; Fortaleza, Leila Yasmim Maria Cidrão

Revista Brasileira de Cirurgia Plástica

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dec 2011, Volume 26 N. 4 Pages 649 - 654

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

INTRODUÇÃO: Cada vez mais são realizadas reconstruções imediatas da mama pós-tratamento do câncer. Para as mastectomias radicais, há várias técnicas consagradas. Na quadrantectomia, as opções de reparação do defeito vão do fechamento primário à utilização de técnicas de cirurgia oncoplástica. Para os tumores dos quadrantes externos, a opção de rotação de todo o músculo latíssimo do dorso é desnecessária, uma vez que usualmente excede ao necessário à reparação do volume perdido. Neste trabalho é proposta uma nova opção para a reconstrução pós-quadrantectomia em quadrantes externos. MÉTODO: A técnica cirúrgica consiste na dissecção do segmento lateral do músculo latíssimo do dorso pelo prolongamento da incisão para a retirada da lesão, no mesmo decúbito dorsal, seguida de sua rotação e modelagem para reparar o defeito decorrente da extirpação do câncer. No total, 21 mulheres com idade entre 33 anos e 69 anos foram submetidas a quadrantectomias de quadrantes externos, seguidas da reconstrução com a técnica descrita neste trabalho. Para a avaliação do método, cada resultado teve atribuído um escore a volume, forma, simetria e alterações do complexo areolopapilar, da cicatriz e da superfície do quadrante operado. RESULTADOS: Os resultados obtidos na avaliação do volume e da forma foram superiores em relação aos da simetria. A nota média foi 7,048, equivalente ao conceito B. CONCLUSÕES: A nova técnica propiciou diminuição do tempo cirúrgico. A aquisição do conceito B demonstra a viabilidade do método.

BACKGROUND: Nowadays, more and more immediate breast reconstructions are carried out after cancer treatment. For performing radical mastectomy, there are several recognized techniques. In quadrantectomy, the options for defect repair range from primary closure to the use of oncoplastic surgery techniques. In the case of external quadrant tumors, there is no option of rotation of the entire latissimus dorsi muscle, which usually exceeds the actions necessary for repairing lost volume. A new option for post-quadrantectomy reconstruction in external quadrants is proposed in this study. METHODS: The surgical technique consists of dissection of the lateral segment of the latissimus dorsi muscle by an extension of the incision for lesion removal in the same supine position, followed by rotation and modeling of this segment to repair the defect caused by cancer removal. Twenty-one women aged 33 to 69 years underwent quadrantectomies of external quadrants followed by reconstruction with the technique described in this paper. To evaluate the method, each result had a score attributed to volume, shape, symmetry, and changes in the nipple areolar complex, as well as scar and operated quadrant surface. RESULTS: The obtained results of volume and shape were superior in relation to those of symmetry. The average grade was 7.048, equivalent to B concept. CONCLUSIONS: The new technique decreased surgical time. The acquisition of the B concept demonstrates the method's feasibility.

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15.

The impact of previous para-areolar incision in the upper outer quadrant of the breast on the localization of the sentinel lymph node in a canine model
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Vasques, Paulo Henrique Diógenes

; Pinheiro, Luiz Gonzaga Porto

; Silva, João Marcos de Meneses e

; Torres-de-Melo, José Ricardo de Moura

; Pinheiro, Karine Bessa Porto

; Rocha, João Ivo Xavier

Clinics

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2011, Volume 66 N. 8 Pages 1413 - 1418

Abstract: En | Text: En | PDF: En

OBJECTIVES: This paper discusses the influence of a para-areolar incision in the upper outer quadrant of the breast on the location of the sentinel lymph node in a canine model. METHODS: The sentinel lymph node was marked with technetium-99, which was injected into the subareolar skin of the cranial breast. After the marker had migrated to the axilla, an arcuate para-areolar incision was performed 2 cm from the nipple in the upper outer quadrant. Patent blue dye was then injected above the upper border of the incision. At the marked site, an axillary incision was made, and the sentinel lymph node was identified by gamma probe and/or by direct visualization of the dye. The agreement between the two injection sites and the two sentinel lymph node identification methods was determined. Our sample group consisted of 40 cranial breasts of 23 adult females of the species Canis familiaris. The data were analyzed by using the McNemar test and by determining the kappa agreement coefficient. RESULT: Our findings showed that in 95% of the breasts, the sentinel lymph node was identified by the injection of technetium-99 m into the subareolar region, and in 82% of the cases, the sentinel lymph node was identified by the injection of patent blue dye above the upper border of the incision. The methods agreed 82% of the time. CONCLUSIONS: Previous para-areolar incisions in the upper outer quadrant did not interfere significantly with the biopsy when the dye was injected above the upper border of the incision.

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You can enrich your search in a very simple way. Use the search indexes combined with the connectors (AND or OR) and specify more your search.

For example, if you want to search for articles about
cases of dengue in Brasil in 2015, use:
ti:dengue and publication_year:2015 and aff_country:Brasil

See below the complete list of search indexes that can be used:

Index code	Element
ti	article title
au	author
kw	article keywords
subject	subject (title words, abstract and keywords)
ab	abstract
ta	journal short title (e.g. Cad. Saúde Pública)
journal_title	journal full title (e.g. Cadernos de Saúde Pública)
la	publication language code (e.g. pt - Portuguese, es - Spanish)
type	document type
pid	publication identifier
publication_year	publication year of publication
sponsor	sponsor
aff_country	country code of the author's affiliation
aff_institution	author affiliation institution
volume	article volume
issue	article issue
elocation	elocation
doi	DOI number
issn	journal ISSN
in	SciELO colection code (e.g. scl - Brasil, col - Colômbia)
use_license	article usage license code

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