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RESUMO Racional: Carcinoma incidental da vesícula biliar é definido como uma neoplasia descoberta por exame histológico após colecistectomia videolaparoscópica. É potencialmente uma doença curável. Entretanto algumas questões relacionadas ao seu manuseio permanecem controversas e uma estratégia definida está associada com melhor prognóstico. Objetivo: Desenvolver o primeiro consenso baseado em evidências para o manuseio de pacientes com carcinoma incidental da vesícula biliar no Brasil. Métodos: Dezesseis questões foram selecionadas e para responder as questões e 36 membros das sociedades brasileiras e internacionais foram incluídos. As recomendações foram baseadas em evidências da literatura atual. Um relatório final foi enviado para os membros do painel para avaliação de concordância. Resultados: Avaliação intraoperatória da peça cirúrgica, uso de bolsas para retirar a peça cirúrgica e exame histopatológico de rotina, foram recomendados. Avaliação pré-operatória completa é necessária e deve ser realizada assim que o estadiamento final esteja disponível. Avaliação da margem do ducto cístico e biópsia de rotina do linfonodo 16b1 são recomendadas. Quimioterapia deve ser considerada e quimioradioterapia indicada se a margem cirúrgica microscópica seja positiva. Os portais devem ser ressecados excepcionalmente. O estadiamento laparoscópico antes da operação é recomendado, mas o tratamento radical por abordagem minimamente invasiva deve ser realizado apenas em centros especializados em cirurgia hepatopancreatobiliar minimamente invasiva. A extensão da ressecção hepática é aceitável até que seja alcançada a ressecção R0. A linfadenectomia padrão é indicada para tumores iguais ou superiores a T2, mas a ressecção da via biliar não é recomendada de rotina. Conclusões: Recomendações seguras foram preparadas para carcinoma incidental da vesícula biliar, destacando os mais frequentes tópicos do trabalho diário do cirurgião do aparelho digestivo e hepatopancreatobiliar.

ABSTRACT Background: Incidental gallbladder cancer is defined as a cancer discovered by histological examination after cholecystectomy. It is a potentially curable disease. However, some questions related to their management remain controversial and a defined strategy is associated with better prognosis. Aim: To develop the first evidence-based consensus for management of patients with incidental gallbladder cancer in Brazil. Methods: Sixteen questions were selected, and 36 Brazilian and International members were included to the answer them. The statements were based on current evident literature. The final report was sent to the members of the panel for agreement assessment. Results: Intraoperative evaluation of the specimen, use of retrieval bags and routine histopathology is recommended. Complete preoperative evaluation is necessary and the reoperation should be performed once final staging is available. Evaluation of the cystic duct margin and routine 16b1 lymph node biopsy is recommended. Chemotherapy should be considered and chemoradiation therapy if microscopically positive surgical margins. Port site should be resected exceptionally. Staging laparoscopy before reoperation is recommended, but minimally invasive radical approach only in specialized minimally invasive hepatopancreatobiliary centers. The extent of liver resection is acceptable if R0 resection is achieved. Standard lymph node dissection is required for T2 tumors and above, but common bile duct resection is not recommended routinely. Conclusions: It was possible to prepare safe recommendations as guidance for incidental gallbladder carcinoma, addressing the most frequent topics of everyday work of digestive and general surgeons.

https://doi.org/10.1590/0102-672020190001e1496 2194 downloads

Topical application of bFGF on acid-conditioned and non-conditioned dentin: effect on cell proliferation and gene expression in cells relevant for periodontal regeneration
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Rocha, Fernanda Regina Godoy

; Souza, João Antônio Chaves de

; Guimarães-Stabili, Morgana Rodrigues

; Sampaio, José Eduardo Cezar

; Rossa Junior, Carlos

Journal of Applied Oral Science

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dec 2017, Volume 25 N. 6 Pages 689 - 699

Abstract: En | Text: En | PDF: En

Abstract Periodontal regeneration is still a challenge in terms of predictability and magnitude of effect. In this study we assess the biological effects of combining chemical root conditioning and biological mediators on three relevant cell types for periodontal regeneration. Material and Methods: Bovine dentin slices were conditioned with 25% citric acid followed by topical application of basic fibroblast growth factor (bFGF, 10 and 50 ng). We used ELISA to assess the dynamics of bFGF release from the dentin surface and RT-qPCR to study the expression of Runx2, Col1a1, Bglap and fibronectin by periodontal ligament (PDL) fibroblasts, cementoblasts and bone marrow stromal cells (BMSC) grown onto these dentin slices. We also assessed the effects of topical application of bFGF on cell proliferation by quantification of genomic DNA. Results: Acid conditioning significantly increased the release of bFGF from dentin slices. Overall, bFGF application significantly (p<0.05) increased cell proliferation, except for BMSC grown on non-conditioned dentin slices. Dentin substrate discretely increased expression of Col1a1 in all cell types. Expression of Runx2, Col1a1 and Fn was either unaffected or inhibited by bFGF application in all cell types. We could not detect expression of the target genes on BMSC grown onto conditioned dentin. Conclusion: Acid conditioning of dentin improves the release of topically-applied bFGF. Topical application of bFGF had a stimulatory effect on proliferation of PDL fibroblasts, cementoblasts and BMSC, but did not affect expression of Runx2, Col1a1, Bglap and fibronectin by these cells.

https://doi.org/10.1590/1678-7757-2017-0051 922 downloads

Efficacy of a bioactive material and nanostructured desensitizing on dentin hypersensitivity treatment
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BEVILACQUA, Flávia Magnani

; CATELAN, Anderson

; ARAÚJO, Giovana Spagnolo Albamonte

; SARACENI, Cintia Helena Coury

; SAMPAIO, José Eduardo Cézar

Revista de Odontologia da UNESP

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jun 2016, Volume 45 N. 3 Pages 127 - 131

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Introdução A hipersensibilidade dentinária é uma ocorrência frequente na prática clínica; clinicamente caracterizada por dor aguda, curta e temporária, em resposta ao estímulo mecânico, químico, térmico ou osmótico causada pela exposição dentinária. Objetivo Comparar in vivo o efeito de um material bioativo cristalino experimental e um dessensibilizante nanoestruturado na avaliação da dor de pacientes com hipersensibilidade dentinária cervical. Material e método Trinta pacientes foram selecionados para este estudo, que foram randomicamente divididos em dois grupos (n=15) em um desenho experimental de boca dividida. Cada paciente recebeu dois tratamentos: grupo 1 (flúor gel e material bioativo) e grupo 2 (flúor gel e dessensibilizante nanoestruturado). As análises de dor foram realizadas usando uma escala visual analógica, variando de 0-10. A mensuração da dor inicial foi realizada previamente ao tratamento inicial (T0) e novas análises foram realizadas semanalmente durante 3 semanas (T1, T2 e T3) antes da reaplicação dos materiais. A análise final da dor foi realizada 3 meses após o início do tratamento (T4). O grau de redução de dor foi mensurado pela fórmula: T0 - Tperíodo após tratamento. Os dados das mensurações de dor foram analisados pela ANOVA para medidas repetidas 2 critérios e teste de Tukey (α=0,05). Resultado Independente do período de avaliação, não houve diferença estatística entre todos os tratamentos na redução da dor (p>0,05). O grau de dor reduziu significativamente em cada período avaliado para todos os tratamentos testados (p<0,05), aproximadamente três graus após 3 meses. Conclusão Os materiais dessensibilizantes testados foram efetivos na redução da hipersensibilidade dentinária.

Abstract Introduction Dentin hypersensitivity is a frequent occurrence in dental practice. It is clinically characterized by acute, short, and temporary pain in response to mechanical, chemical, thermal, or osmotic stimuli resulting from dentin exposure. Objective To compare in vivo the effect of an experimental crystalline bioactive material and nanostructured desensitizing on the pain assessment among patients with cervical dentin hypersensitivity. Material and method Thirty patients were selected for this study, who were randomly assigned to two groups (n=15) in a split-mouth design. Each patient received two treatments: group 1 (fluoride gel and bioactive material) and group 2 (fluoride gel and nanostructured desensitizing). Pain analyses were performed using a visual analogue scale, ranging 0-10. Baseline pain measurement was performed prior to initial treatment (T0) and new measurements were carried out weekly for 3 weeks (T1, T2, and T3) before materials were reapplied. Final pain analysis was performed 3 months after initial treatment (T4). Degree of pain reduction was calculated using the formula T0 - Tperiod after treatment. The data of the pain measurements were analyzed using 2-way repeated measure ANOVA and Tukey’s test (α=0.05). Result Regardless of evaluation period, there was no statistical difference on pain reduction between the treatments (p>0.05). The degree of pain was reduced significantly in each evaluated period for all tested treatments (p<0.05), by approximately three degrees after 3 months. Conclusion The tested desensitizing materials were effective on dentin hypersensitivity reduction.

https://doi.org/10.1590/1807-2577.24115 1779 downloads

The smear layer created by scaling and root planing is physiologically eliminated in a biphasic process
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ROCHA, Fernanda Regina Godoy

; ZANDIM-BARCELOS, Daniela Leal

; ROSSA JUNIOR, Carlos

; SAMPAIO, José Eduardo Cezar

Brazilian Oral Research

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2015, Volume 29 N. 1 Pages 01 - 7

Abstract: En | Text: En | PDF: En

Mechanical instrumentation of the root surface causes the formation of a smear layer, which is a physical barrier that can affect periodontal regeneration. Although different procedures have been proposed to remove the smear layer, there is no information concerning how long the smear layer persists on root surfaces after instrumentation in vivo. This study assessed the presence of the smear layer on root surfaces over a 28-day period after subgingival instrumentation with hand instruments. Fifty human teeth that were referred for extraction because of advanced periodontal disease were scaled and root planed (SRP) by a single experienced operator. Ten teeth were randomly assigned to be extracted 7, 14, 21, and 28 days after SRP. Another 10 teeth were extracted immediately after instrumentation (Day 0, control group). The subgingival area of the instrumented roots was evaluated with scanning electron microscopy. Representative photomicrographs were assessed by a blinded and calibrated examiner according to a scoring system. A rapid and significant (p < 0.05, Z test) initial reduction in the amount of smear layer was observed at 7 days, and a further significant (p < 0.05) decrease was observed 28 days after SRP. Interestingly, even 28 days after SRP, the smear layer was still present on root surfaces. This study showed that the physiological elimination of the smear layer occurred in a biphasic manner: a rapid initial reduction was observed 7 days after instrumentation, which was followed by a slow process leading to a significant decrease 28 days after instrumentation.

https://doi.org/10.1590/1807-3107BOR-2015.vol29.0029 3307 downloads

Blood clot stabilization on root dentin conditioned by the combination of tetracycline and EDTA
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Nascimento, Gustavo Giacomelli

; Leite, Amauri Antiquera

; Leite, Elza Regina Manzolli

; Sampaio, José Eduardo Cezar

; Leite, Fábio Renato Manzolli

Brazilian Journal of Oral Sciences

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jun 2014, Volume 13 N. 2 Pages 83 - 88

Abstract: En | Text: En | PDF: En

AIM: To assess the combined use of tetracycline (TTC) and ethylenediaminetetraacetic acid (EDTA) on clot formation, considering that EDTA may neutralize TTC acidity.METHODS: Planed human tooth roots were treated with saline solution, EDTA, TTC and their combination (EDTA followed by TTC and TTC before EDTA). Fresh human blood was applied on the conditioned surfaces to check clot adhesion and stabilization. A previously calibrated (kappa = 0.93) and blinded examiner scored scanning electron micrographs of the samples. Statistical analyses were performed using one-way ANOVA and Tukey's test.RESULTS: Application of TTC before EDTA presented the best results with the highest number of cells adhered to the root surface (p=0.046). Use of EDTA alone and EDTA before TTC disturbed clot stabilization when compared to control group (p<0.01).CONCLUSIONS:The use of TTC before EDTA seems to be able to keep blood cells viable to establish an organized clot and could be used by clinicians together with the conventional mechanical root scaling and planing.

https://doi.org/10.1590/1677-3225v13n2a01 1697 downloads

In vitro evaluation of Biosilicate® dissolution on dentin surface: a SEM analysis
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Pinheiro, Michele Carolina

; Lopes, Beatriz Maria Valério

; Cavassim, Rodrigo

; Pinto, Shelon Cristina Souza

; Sampaio, José Eduardo Cezar

Revista de Odontologia da UNESP

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aug 2013, Volume 42 N. 4 Pages 251 - 258

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

INTRODUÇÃO: Biomateriais, tais como vidros bioativos e vidros cerâmicos têm sido propostos para o tratamento da hipersensibilidade dentinária. OBJETIVO: Avaliar por microscopia eletrônica de varredura (SEM), a dissolução de uma nova vitrocerâmica bioativa (Biosilicato®, partículas de 1-20 µm) na superfície de amostras de dentina, com diferentes métodos de aplicação e de meio de diluição diferente, usado para aplicação de Biosilicato®. MATERIAL E MÉtODO: 280 amostras de dentina foram divididas aleatoriamente em quatro grupos: (1) Biosilicato® mais flúor gel aplicado com escova de Robinson, (2) Biosilicato® mais flúor gel aplicado com microbrush, (3) Biosilicato® mais água destilada aplicado com escova de Robinson; (4) Biosilicato® mais água destilada aplicado com microbrush. Após o tratamento, as amostras foram imersas em saliva, em diferentes períodos (0, 30 e 15 minutos, 1, 2, 12 e 24 horas). Duas fotomicrografias foram obtidas a partir de cada amostra e foram analisadas por um examinador calibrado cego de acordo com um "Índice de Dissolução de Partículas", criado para este estudo. RESULTADO: Os dados foram analisados usando o teste de Mann-Whitney, Kruskal-Wallis e Dunn. Não houve diferença estatística entre os graus de dissolução entre os quatro grupos em qualquer período. CONCLUSÃO: Biosilicato® pode ser incorporado em ambas às substâncias, sem diferenças no grau de dissolução das partículas em qualquer dos períodos de avaliação e a aplicação sobre da dentina pode ser realizada com os dois métodos avaliados.

INTRODUCTION: Biomaterials such as bioactive glasses and glass-ceramics have been proposed for the treatment of dentinal hypersensitivity. OBJECTIVE: to evaluate by scanning electron microscopy (SEM), the dissolution of a novel bioactive glass-ceramic (Biosilicate® 1-20 µm particles) on dentin surface samples, with different application methods and different dilution medium used for applying Biosilicate®. MATERIAL AND METHOD: 280 dentin samples were randomly divided into four groups: (1) Biosilicate® plus fluoride gel applied with Robinson brush; (2) Biosilicate® plus fluoride gel applied with microbrush; (3) Biosilicate® plus distilled water applied with Robinson brush; (4) Biosilicate® plus distilled water applied with microbrush. After treatment, the samples were immersed in saliva at different periods (0, 15 and 30 minutes, 1, 2, 12 and 24 hours). Two photomicrographs were obtained from each sample and were further analyzed by a blind calibrated examiner according to a "Particle Dissolution Index" created for this study. RESULT: The data were analyzed using the Mann-Whitney, Kruskal-Wallis and Dunn's tests. There was no statistical difference among the degrees of dissolution between the 4 groups in any period. CONCLUSION: Biosilicate® can be incorporated in both substances without differences in the degree of dissolution of the particles in any of the evaluated periods and the application of dentine can be performed with both methods evaluated.

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Comparação in vitro da eficácia de diferentes formulações do gel de EDTA 24% no condicionamento da superfície radicular
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Sousa, Cliciane Portela

; Frizzera, Fausto

; Batista, Luiz Henrique Carvalho

; Dantas, Andrea Abi Rached

; Zandim-Barcelos, Daniela Leal

; Sampaio, José Eduardo Cezar

Revista de Odontologia da UNESP

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feb 2013, Volume 42 N. 1 Pages 7 - 12

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

INTRODUÇÃO: A biomodificação radicular visa a alterar a superfície radicular para auxiliar no reparo das estruturas periodontais de suporte destruídas pela doença periodontal. OBJETIVO: Avaliar por meio de microscopia eletrônica de varredura a eficácia do condicionamento químico com gel de EDTA 24% na remoção de smear layer e na exposição de fibras colágenas, verificando-se diferentes marcas comerciais, modos e tempos de aplicação. MATERIAL E MÉTODO: Um total de 225 amostras foram obtidas e distribuídas aleatoriamente em cinco grupos: soro fisiológico (controle); EDTA 24% (Farmácia Santa Paula); EDTA-T 24% (Farmácia Santa Paula); EDTA 24% (PrefGel; Straumann); EDTA 24% (Biodinâmica). As amostras de cada grupo foram redistribuídas aleatoriamente em subgrupos (n = 5) e condicionadas com os diferentes géis de EDTA nos tempos de 1, 2 e 3 minutos, e nos modos de aplicação tópica, fricção vigorosa e fricção suave. Os testes de Kruskal-Wallis e Dunn (p < 0,05) foram utilizados e aplicados separadamente para os fatores: marca comercial, tempo e modo de aplicação. RESULTADO: A análise estatística revelou que o gel de EDTA 24% da Biodinâmica promoveu a menor remoção de smear layer. Não foram detectadas diferenças estatísticas para os tempos de aplicação. O modo de aplicação fricção suave resultou em menor quantidade de smear layer residual. CONCLUSÃO: Todos os géis de EDTA 24% utilizados foram eficazes na remoção de smear layer e na exposição de fibras colágenas, independentemente do tempo de aplicação; no entanto, o modo de fricção suave foi mais efetivo. Na análise comparativa entre os produtos, o EDTA da Biodinâmica foi menos efetivo, já que promoveu menor remoção de smear layer e exposição de fibras colágenas.

INTRODUCTION: The main goal of root biomodification is to modify the root surface in order to improve the repair of periodontal tissues destroyed by periodontal disease. OBJECTIVE: To evaluate the conditioning efficacy of 24% EDTA gel of different trademarks, considering the variables time and application method, by scanning electron microscopy. MATERIAL AND METHOD: 225 samples were randomly assigned to five groups: sterile saline solution (control); 24% EDTA (Santa Paula Pharmacy); 24% EDTA-T (Santa Paula Pharmacy); 24% EDTA (PrefGel - Straumann); 24% EDTA (Biodinâmica). For each experimental group the gels were applied for 1, 2 or 3 minutes by topical application; vigorous or smooth friction. The Kruskal-Wallis and Dunn tests (p < 0,05) were used and individually applied to the variables commercial brand, time and type of application. RESULT: The statistical analysis showed that 24% EDTA Biodinâmica gel promoted less smear layer removal compared to the other groups. No significant differences could be detected among the different times of application. The smooth friction resulted in lower amount of residual smear layer. CONCLUSION: All 24% EDTA gels tested were able to remove smear layer and to expose dentinal tubules irrespective of application time; however, the application method by smooth friction was more effective than the other ones. The comparative analysis demonstrated that the Biodinâmica gel was the least effective product, promoting less smear layer removal and exposure of collagen fibers than the other EDTA gels.

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Influence of concentration, time and method of application of citric acid and sodium citrate in root conditioning
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Cavassim, Rodrigo

; Leite, Fábio Renato Manzolli

; Zandim, Daniela Leal

; Dantas, Andrea Abi Rached

; Rached, Ricardo Samih Georges Abi

; Sampaio, José Eduardo Cezar

Journal of Applied Oral Science

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jun 2012, Volume 20 N. 3 Pages 376 - 383

Abstract: En | Text: En | PDF: En

OBJECTIVES: The aim of this study was to establish the parameters of concentration, time and mode of application of citric acid and sodium citrate in relation to root conditioning. MATERIAL AND METHODS: A total of 495 samples were obtained and equally distributed among 11 groups (5 for testing different concentrations of citric acid, 5 for testing different concentrations of sodium citrate and 1 control group). After laboratorial processing, the samples were analyzed under scanning electron microscopy. A previously calibrated and blind examiner evaluated micrographs of the samples. Non-parametric statistical analysis was performed to analyze the data obtained. RESULTS: Brushing 25% citric acid for 3 min, promoted greater exposure of collagen fibers in comparison with the brushing of 1% citric acid for 1 minute and its topical application at 1% for 3 min. Sodium citrate exposed collagen fibers in a few number of samples. CONCLUSION: Despite the lack of statistical significance, better results for collagen exposure were obtained with brushing application of 25% citric acid for 3 min than with other application parameter. Sodium citrate produced a few number of samples with collagen exposure, so it is not indicated for root conditioning.

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Effect of Er,Cr:YSGG and Er:YAG laser irradiation on the adhesion of blood components on the root surface and on root morphology
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Oliveira, Guilherme José Pimentel Lopes de

; Theodoro, Letícia Helena

; Marcantonio Junior, Elcio

; Sampaio, José Eduardo Cezar

; Marcantonio, Rosemary Adriana Chiérici

Brazilian Oral Research

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jun 2012, Volume 26 N. 3 Pages 256 - 262

Abstract: En | Text: En | PDF: En

The aim of this study was to conduct an in vitro evaluation, by scanning electron microscopy (SEM), of the adhesion of blood components on root surfaces irradiated with Er,Cr:YSGG (2.78 µm) or Er:YAG (2.94 µm) laser, and of the irradiation effects on root surface morphology. Sixty samples of human teeth were previously scaled with manual instruments and divided into three groups of 20 samples each: G1 (control group) - no treatment; G2 - Er,Cr:YSGG laser irradiation; G3 - Er:YAG laser irradiation. After performing these treatments, blood tissue was applied to 10 samples of each group, whereas 10 samples received no blood tissue application. After performing the laboratory treatments, the samples were observed under SEM, and the resulting photomicrographs were classified according to a blood component adhesion scoring system and root morphology. The results were analyzed statistically (Kruskall-Wallis and Mann Whitney tests, α= 5%). The root surfaces irradiated with Er:YAG and Er,Cr:YSGG lasers presented greater roughness than those in the control group. Regarding blood component adhesion, the results showed a lower degree of adhesion in G2 than in G1 and G3 (G1 × G2: p = 0.002; G3 × G2: p = 0.017). The Er:YAG and Er,Cr:YSGG laser treatments caused more extensive root surface changes. The Er:YAG laser treatment promoted a greater degree of blood component adhesion to root surfaces, compared to the Er,Cr:YSGG treatment.

3984 downloads

10.

Effect of acid fruit juices combined with electric or sonic toothbrushing on root dentin permeability: an in vitro study
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Batitucci, Roberta Grasselli

; Zandim, Daniela Leal

; Rocha, Fernanda Regina Godoy

; Pinheiro, Michele Carolina

; Fontanari, Lucas Amaral

; Sampaio, José Eduardo Cezar

Brazilian Dental Journal

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2012, Volume 23 N. 6 Pages 667 - 671

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

O objetivo deste trabalho in vitro foi quantificar as alterações na permebilidade da dentina radicular humana após exposição a diferentes sucos de frutas ácidas e avaliar o efeito da escovação, com escova elétrica ou sônica, após a exposição ácida. A dentina radicular de 50 terceiros molares foi exposta com a utilização de fresas em alta rotação. As coroas foram seccionadas acima da junção cemento-esmalte e apenas os fragmentos radiculares foram utilizados no preparo dos espécimes. Os espécimes foram aleatoriamente divididos em 5 grupos de acordo com o suco de fruta aplicado (kiwi, carambola, maça verde, abacaxi e acerola). Cada espécime foi conectado a um aparelho de pressão para medir a permeabilidade dentinária por meio do método de filtração de líquidos após as seguintes etapas sequências: I) condicionamento com ácido fosfórico 37% durante 30 s, II) raspagem da raiz, III) exposição aos sucos de frutas por 5 min, IV) escovação com escova elétrica ou sônica durante 3 min. Os dados foram analisados estatisticamente pelos testes Wilcoxon e Mann-Whitney com nível de significância de 5%. Os resultados mostraram que todos os sucos de frutas testados promoveram aumento significativo da permeabilidade dentinária e os procedimentos de escovação causaram diminuição. Pode-se concluir que os sucos de frutas ácidas aumentaram a permeabilidade da dentina radicular, enquanto que a escovação sem dentifrício imediatamente após a exposição ácida promoveu redução da permeabilidade. Além disso, o mecanismo da escova (elétrica ou sônica) não teve influência na redução da permeabilidade dentinária.

The purpose of this in vitro study was to quantify the alterations on human root dentin permeability after exposure to different acid fruit juices and to evaluate the effect of toothbrushing with electric or sonic toothbrush after acid exposure. The root dentin of 50 extracted third molars was exposed with a high speed bur. Crowns were sectioned above the cementoenamel junction and root fragments were used to prepare dentin specimens. Specimens were randomly assigned to 5 groups according to the fruit juice (kiwifruit, starfruit, green apple, pineapple and acerolla). Each specimen was connected to a hydraulic pressure apparatus to measure root dentin permeability using fluid filtration method after the following sequential steps: I) conditioning with 37% phosphoric acid for 30 s, II) root scaling, III) exposure to acid fruit juices for 5 min and IV) electric or sonic toothbrushing without dentifrice for 3 min. Data were analyzed statistically by the Wilcoxon and Mann-Whitney tests at 5% significance level. All fruit juices promoted a significant increase of dentin permeability while toothbrushing decreased it significantly (p<0.05). It may be concluded that all acid fruit juices increased root dentin permeability, while toothbrushing without dentifrice after acid exposure decreased the permeability. The toothbrush mechanism (electric or sonic) had no influence on the decrease of root dentin permeability.

3552 downloads

11.

Effect of instrumentation using curettes, piezoelectric ultrasonic scaler and Er,Cr:YSGG laser on the morphology and adhesion of blood components on root surfaces: a SEM study
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Tsurumaki, Jackeline do Nascimento

; Souto, Bráulio Henrique Marques

; Oliveira, Guilherme José Pimentel Lopes de

; Sampaio, José Eduardo Cézar

; Marcantonio Júnior, Elcio

; Marcantonio, Rosemary Adriana Chiérici

Brazilian Dental Journal

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2011, Volume 22 N. 3 Pages 185 - 192

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Esse estudo utilizou microscopia eletrônica de varredura (MEV) para avaliar a morfologia e a adesão de elementos sanguíneos em superfícies radiculares instrumentadas com curetas, ultrassom piezoelétrico e laser de Er,Cr:YSGG. Foram utilizadas no presente estudo 100 amostras provenientes de 25 dentes que foram divididas em 5 grupos: 1) Raspagem manual com curetas; 2) Raspagem com ultrassom; 3) Associação instrumento manual e ultrassom; 4)Irradiação do laser de Er,Cr:YSGG;5)Associação raspagem manual com irradiação com laser de Er,Cr:YSGG. Dez amostras de cada grupo foram utilizadas para análise da morfologia e as outras 10 foram utilizadas para a análise de adesão de elementos sanguíneos. As eletromicrografias foram analisadas através dos escores de adesão de elementos sanguíneos e pelo índice de morfologia radicular e os resultados foram analisados estatisticamente através dos testes de Kruskall-Wallis e de Mann-Whitney com nível de significância de 5%. O grupo que foi tratado com instrumentos manuais apresentou superfície mais lisa em relação aos grupos que foram instrumentados com ultrassom e com o laser de Er,Cr:YSGG. As superfícies instrumentadas com ultrassom e com o laser de Er,Cr:YSGG de forma isolada ou associada a raspagem manual não apresentaram diferenças estatísticas entre si (p>0,05). Não houve diferenças estatísticas entre os grupos em relação a adesão de elementos sanguíneos(p>0,05). A instrumentação ultrassônica e a irradiação com o laser de Er,Cr:YSGG produziram superfícies radiculares mais rugosas em relação a raspagem com curetas, porém não houve diferenças entre os tratamentos com relação à adesão de elementos sanguíneos.

This study used scanning electron microscopy (SEM) to evaluate the morphology and adhesion of blood components on root surfaces instrumented by curettes, piezoelectric ultrasonic scaler and Er,Cr:YSGG laser. One hundred samples from 25 teeth were divided into 5 groups: 1) Curettes; 2) Piezoelectric ultrasonic scaler; 3) Curettes plus piezoelectric ultrasonic scaler; 4) Er,Cr:YSGG laser; 5) Curettes plus Er,Cr:YSGG laser. Ten samples from each group were used for analysis of root morphology and the other 10 were used for analysis of adhesion of blood components on root surface. The results were analyzed statistically by the Kruskall-Wallis and Mann-Whitney tests with a significance level of 5%. The group treated with curettes showed smoother surfaces when compared to the groups were instrumented with piezoelectric ultrasonic scaler and the Er,Cr:YSGG laser. The surfaces instrumented with piezoelectric ultrasonic scaler and Er,Cr:YSGG laser, alone or in combination with hand scaling and root planing, did not differ significantly (p>0.05) among themselves. No statistically significant differences (p>0.05) among groups were found as to the adhesion of blood components on root surface. Ultrasonic instrumentation and Er,Cr:YSGG irradiation produced rougher root surfaces than the use of curettes, but there were no differences among treatments with respect to the adhesion of blood components.

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12.

Morphological alterations on human radicular dentin after exposure to different fruit juice drinks
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Zandim, Daniela Leal

; Martins, Cínthia Oliveira

; Cavassim, Rodrigo

; Rossa Júnior, Carlos

; Abi-Rached, Ricardo Samih Georges

; Sampaio, José Eduardo Cezar

Revista Odonto Ciência

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2011, Volume 26 N. 1 Pages 65 - 70

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

OBJETIVO: O objetivo deste estudo in vitro foi avaliar o efeito de diferentes sucos de fruta disponíveis no mercado brasileiro sobre o grau de remoção de smear layer e abertura dos túbulos dentinários, assim como verificar o efeito da escovação realizada imediatamente após a exposição aos sucos. METODOLOGIA: Amostras de dentina foram preparadas e distribuídas aleatoriamente entre o grupo controle (água destilada) e doze tipos de suco de fruta (caju, laranja, tangerina, maçã, maracujá, goiaba, morango, uva, manga, pêra, pêssego, abacaxi). Os tratamentos aplicados foram: imersão ou imersão + escovação. Após preparo para MEV, fotomicrografias foram avaliadas utilizando-se um índice de remoção de smear layer. RESULTADOS: Não foram encontradas diferenças significativas entre os grupos em relação à remoção de smear layer e abertura dos túbulos após ambos os tratamentos (Kruskal-Wallis, comparações pareadas, P>0.05). A solução controle e os sucos de fruta não promoveram remoção de smear layer e abertura dos túbulos dentinários. Apenas no grupo do suco de manga foi verificada uma diferença significativa entre os tratamentos aplicados (Mann-Whitney, P<0.05). CONCLUSÃO: Com base na metodologia utilizada, pode-se concluir que os diferentes sucos de fruta não promoveram alterações significativas na morfologia da dentina radicular apesar da realização subsequente de procedimentos de escovação.

PURPOSE: This in vitro study aimed to evaluate the effect of different fruit juice drinks available in the Brazilian market on smear layer removal and dentinal tubules opening, as well as to verify the effect of toothbrushing subsequently to the juices exposure. METHODS: Dentin specimens were prepared and randomly distributed into the control group (distilled water) and twelve types of fruit juice drinks (cashew, orange, mandarin, apple, passion fruit, guava, strawberry, grape, mango, pear, peach, pineapple). The following treatments were applied: immersion or immersion + brushing. After preparation for SEM, photomicrographs were assessed using an index of smear layer removal. RESULTS: No significant differences regarding smear layer removal and dentinal tubules exposure could be observed between the groups after both treatments (Kruskal-Wallis, post-hoc paired comparisons, P>0.05). The control solution and the fruit juice drinks were not able to remove smear layer and to open dentinal tubules. Significant difference between the applied treatments was detected only for the mango juice group (Mann-Whitney, P<0.05). CONCLUSION: Under the experimental conditions, the different fruit juice drinks did not promote significant alterations on human radicular dentin morphology regardless of the subsequent application of brushing procedures.

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13.

Influence of dental exposure to oral environment on smear layer removal and collagen exhibition after using different conditioning agents
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Fontanari, Lucas Amaral

; Pinto, Shelon Cristina Souza

; Cavassim, Rodrigo

; Spin-Neto, Rubens

; Ishi, Eduardo de Paula

; Sampaio, José Eduardo Cezar

Brazilian Dental Journal

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2011, Volume 22 N. 6 Pages 479 - 485

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Apesar de estudos in vitro terem demonstrado resultados favoráveis ao condicionamento da superfície radicular com agentes desmineralizantes, estudos in vivo não mostraram seus benefícios na cicatrização periodontal. Isto pode ser atribuído a vários fatores, entre os quais, a hipermineralização da superfície dental. Portanto, este estudo in vitro comparou, usando microscopia eletrônica de varredura, o efeito do condicionamento da superfície radicular com diferentes condicionadores (1% e 25% de ácido cítrico, EDTA 24% e 50 mg/mL de cloridrato de tetraciclina) em dentes impactados e dentes que tinham suas raízes expostas ao meio bucal. Um examinador treinado avaliou as microscopias eletrônicas de varredura utilizando um índice de modificação da superfície radicular. Houve uma tendência de maior modificação da superfície radicular no grupo de dentes impactados, sugerindo que o grau de mineralização da raiz influencia a sua desmineralização química.

Although in vitro studies have shown encouraging results for root surface conditioning with demineralizing agents, in vivo studies have failed to show its benefits in periodontal healing. This can be attributed to several factors, among which, the hypermineralization of dental surface. Therefore, this in vitro study compared, using scanning electron microscopy (SEM), the effect of root surface conditioning with different conditioners (1% and 25% citric acid, 24% EDTA and 50 mg/mL tetracycline hydrochloride) in impacted teeth and in teeth that had their roots exposed to the oral environment. One trained examiner assessed the SEM micrographs using a root surface modification index. There was a tendency of more root surface modification in the group of impacted teeth, suggesting that the degree of root mineralization influences its chemical demineralization.

3133 downloads

14.

Effect of an acid diet allied to sonic toothbrushing on root dentin permeability: an in vitro study
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Pinto, Shelon Cristina Souza

; Batitucci, Roberta Grasseli

; Pinheiro, Michele Carolina

; Zandim, Daniela Leal

; Spin-Neto, Rubens

; Sampaio, José Eduardo Cezar

Brazilian Dental Journal

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2010, Volume 21 N. 5 Pages 390 - 395

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Este estudo quantificou a alteração na permeabilidade da dentina radicular submetida a diferentes dietas ácidas. Vinte e cinco terceiros molares foram seccionados abaixo da junção cemento-esmalte, a raiz seccionada foi selecionada, e a polpa removida. A seguir, as amostras foram conectadas a um aparato de pressão hidráulica para medir a permeabilidade da dentina radicular de acordo com os seguintes passos: I) tratamento com ácido fosfórico por 30 s (permeabilidade máxima), II) aplainamento radicular para criar nova smear layer, III) exposição a diferentes substâncias ácidas por 5 min (suco de laranja, coca-cola, vinagre, vinho branco e suco de limão), IV) escovação com escova sônica por 3 min, V) escovação com escova sonica mais dentifrício, por 3 min. Os dados foram convertidos em porcentagem em relação ao passo I (100%) e cada amostra foi seu próprio controle. Os resultados mostraram que todas as substâncias ácidas aumentaram significantemente a permeabilidade dentinária após raspagem. Escovação após a exposição a substâncias ácidas diminuiu a permeabilidade e a associação com dentifrício aumentou a redução na permeabilidade dentinária; essa redução adicional não foi observada somente nas amostras tratadas com coca-cola. Com isso, pode-se concluir que todas as substâncias ácidas testadas aumentaram a permeabilidade dentinária, e a escovação com ou sem dentifrício pode diminuir a permeabilidade dentinária radicular após a exposição da dentina a dieta ácida.

This study quantified alterations in root dentin permeability after exposure to different acid beverages. Twenty-five third molars were sectioned below the cementoenamel junction, the root segment was collected, and the pulp tissue was removed. The root segments were connected to a hydraulic pressure apparatus to measure the permeability of root dentin after the following sequential steps, with 5 specimens in each: 1) phosphoric acid etching for 30 s (maximum permeability), 2) root planning to create new smear layer, 3) exposure to different acid substances for 5 min (orange, cola drink, vinegar, white wine, lemon juice), 4) toothbrushing with sonic toothbrush for 3 min, 5) toothbrushing with sonic toothbrush plus dentifrice for 3 min. Considering step I as 100%, the data were converted into percentage and each specimen was its own control. Data were analyzed statistically by Kruskal-Wallis and Dunn's post test at 5% significance level. All acidic substances increased dentin permeability significantly after scraping (p< 0.05). Toothbrushing after exposure to acid substances decreased dentin permeability and the association with dentifrice accentuated the decrease (p< 0.05), except for the specimens treated with cola drink. Thus, it may be concluded that all tested acid fruit juices increased dentin permeability, and toothbrushing with or without dentifrice can decrease root dentin permeability after dentin exposure to acid diet.

2934 downloads

15.

In vitro evaluation of the effect of natural orange juices on dentin morphology
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Zandim, Daniela Leal

; Corrêa, Fernanda Oliveira Bello

; Rossa Júnior, Carlos

; Sampaio, José Eduardo Cezar

Brazilian Oral Research

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jun 2008, Volume 22 N. 2 Pages 176 - 183

Abstract: En | Text: En | PDF: En

The patient's diet has been considered an important etiological factor of dentin hypersensitivity. The frequent ingestion of acidic substances can promote the loss of dental structure or remove the smear layer. The purpose of this study was to evaluate the degree of smear layer removal and dentinal tubules exposure by different natural orange juices. Extracted human teeth were submitted to manual scaling in order to develop the smear layer. Seventy dentin samples were obtained and distributed into the following groups: Control, lime orange, lime, valência orange, navel orange, mandarin, and tangerine. Each group included 2 methods of application: Topical and topical + friction. After preparation for SEM analysis, photomicrographs were assessed by a blind calibrated examiner using an index system. The Kruskal-Wallis test indicated a significant influence of the orange juices on smear layer removal. Significant difference was observed between navel orange, valência orange, mandarin and the control group (p < 0.05). These orange juices resulted in greater removal of the smear layer and greater opening of dentinal tubules. The comparison between the application methods for each group using the Mann-Whitney test showed that friction increased smear layer removal significantly only for lime orange and lime. The data suggest that certain natural orange juices are more effective in terms of smear layer removal and dentinal tubules exposure than others.

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