Abstract INTRODUCTION: The state of São Paulo has been monitoring cases of microcephaly and pregnant women presenting with acute rash, through CeVeSP. METHODS: This was a descriptive study focusing on pregnant women with rash and the outcome of their pregnancy, based on the notifications through the CeVeSP. RESULTS: During 2016, 2,209 cases of pregnant women with rash were reported and investigated. Of these, 36.6% were confirmed. Of the pregnant women who tested positive for ZIKV, 6.4% did not have a favorable outcome. CONCLUSIONS Our results allowed the characterization of pregnant women exposed to ZIKV and the outcome of pregnancy.

OBJECTIVE: The preservation of biological samples at a low temperature is important for later biochemical and/or histological analyses. However, the molecular viability of thawed samples has not been studied sufficiently in depth. The present study was undertaken to evaluate the viability of intact tissues, tissue homogenates, and isolated total RNA after defrosting for more than twenty-four hours. METHODS: The molecular viability of the thawed samples (n = 82) was assessed using the A260/A280 ratio, the RNA concentration, the RNA integrity, the level of intact mRNA determined by reverse transcriptase polymerase chain reaction, the protein level determined by Western blotting, and an examination of the histological structure. RESULTS: The integrity of the total RNA was not preserved in the thawed intact tissue, but the RNA integrity and level of mRNA were perfectly preserved in isolated defrosted samples of total RNA. Additionally, the level of β-actin protein was preserved in both thawed intact tissue and homogenates. CONCLUSION: Isolated total RNA does not undergo degradation due to thawing for at least 24 hours, and it is recommended to isolate the total RNA as soon as possible after tissue collection. Moreover, the protein level is preserved in defrosted tissues.

Peripheral vascular catheters are frequent in the patients therapeutic and if it is not installed and handled observing the asepsis principles they can represent infection risk. The objective of this study was to verify the adhesion to the aseptic measures for the vascular access. The study was carried out in six hospitals of Goiânia-GO. The population consists of professionals of the nursing team who work in Surgery Clinics and ICU. Data was collected through observation and registered in a check list. In the 209 observed chances the professionals neglected basic measures for the prevention of infections related to the vascular access. Such measures include the hand hygiene, the use of gloves, the antisepsis of the skin, maintenance of venous infusion in closed system and the adoption of aseptic measures in the medicine preparation. We believe to be essential to invest in the qualification of the professionals and standardization of behaviors, and the implementation of policies of protection to the workers which includes investing in safer equipments.

Los catéteres vasculares periféricos son frecuentes en la terapéutica de los pacientes, y si no instalados y manipulados observando los principios de la asepsia pueden representar riesgo de infección. El objetivo de este estudio fue verificar la adherencia a las medidas asépticas para el acceso vascular. Realizado en seis hospitales de Goiânia-GO, y la población se constituyó de profesionales del equipo de Enfermería que actúan en las Clínicas Quirúrgicas y en las Unidades de Cuidados Intensivos (UCIs). Los datos fueron recogidos por medio de observación y registros en check list. En las 209 oportunidades, los profesionales descuidaron medidas fundamentales para la prevención de infecciones relacionadas al acceso vascular. Estas medidas incluyeron la higienización de las manos, el uso de guantes, la antisepsia de la piel, la manutención de infusión venosa en sistema cerrado y la adopción de medidas asépticas en el preparo de la medicación. Creemos ser imprescindible invertir en la calificación de los profesionales, estandarización de conductas y en la implementación de política de protección a los trabajadores como inversiones en equipamientos más seguros.

Cateteres vasculares periféricos são freqüentes na terapêutica dos pacientes, e se não instalados e manuseados com observância dos princípios de assepsia podem representar risco de infecção. O objetivo deste estudo foi verificar a adesão às medidas assépticas para o acesso vascular. Ele foi realizado em seis hospitais de Goiânia - GO e a população constituiu-se de profissionais de enfermagem que atuam nas clínicas cirúrgicas e UTIs. Os dados foram coletados por meio de observação e registrados em um check list. Nas 209 oportunidades, os profissionais negligenciaram medidas básicas de prevenção de infecções relacionadas ao acesso vascular. Tais medidas incluíram higiene das mãos, uso de luvas, anti-sepsia da pele, manutenção de infusão venosa em sistema fechado e adoção de medidas assépticas no preparo de medicamentos. Acreditamos ser imprescindível investir em qualificação dos profissionais, padronização de condutas e implementação de política de proteção aos trabalhadores com investimentos em equipamentos mais seguros.

Para avaliar a influência de fatores raciais sobre a função hepática de bovinos foram colhidas 134 amostras de sangue, sendo 67 animais da raça Jersey e 67 da raça Holandesa, criados em São Paulo. No soro sangüíneo realizaram-se as seguintes determinações: proteinograma; atividade enzimática da aspartato aminotransferase (AST), gama glutamiltransferase (GGT), bilirrubinas indireta, direta e total. A avaliação do proteinograma demonstrou a influência dos fatores raciais, pois os valores médios da proteína total dos bovinos da raça Jersey (6,37±0,90 g/dl) foram menores do que os animais da raça Holandesa (6,82±0,97 g/dl).Essas variações foram decorrentes as frações protéicas que correspondem as globulinas, sendo os teores médios obtidos para bovinos da raça Jersey (3,13 ± 0,81g/dl) menores do que os encontrados para bovinos da raça Holandesa (3,73 ± 0,99 g/dl). Os valores das alfa-, beta- e gama-globulinas obtidos nos animais da raça Jersey (iguais a 0,89±0,14 g/dl; 0,71±0,14 g/dl; 2,03±0,38 g/dl) foram menores do que os encontrados para bovinos da raça Holandesa (iguais a 1,00±0,16 g/dl; 0,76±0,15 g/dl; 2,20±0,45 g/dl). A avaliação da atividade enzimática revelou a influência dos fatores raciais nos resultados da AST, sendo os valores dos bovinos Jersey (49,27±17,87 U/l) maiores do que os da raça Holandesa (34,76±10,61 U/l), enquanto para a GGT não foram observadas diferenças significativas entre os resultados da raça Jersey (19,46±33,11 U/l) e da raça Holandesa (19,08±33,26 U/l). A avaliação dos teores séricos de bilirrubinas evidenciou variações sob influência racial para os teores de bilirrubinas indireta e total, sendo os valores obtidos para a raça Jersey (0,40±0,33 mg/dl de bilirrubina indireta e 0,44±0,33 mg/dl de bilirrubina total) maiores do que os observados para a raça Holandesa (0,22±0,19 mg/dl de bilirrubina indireta e 0,25±0,19 mg/dl de bilirrubina total).

With the purpose to evaluate the breed factor inf1uence on the hepatic function of cattle, 134 blood samples from, 67 Jersey and 67 Holstein cattle in São Paulo, Brazil, were collected . The hepatic function was studied through the determination of proteinogram; activities of the aspartate aminotransferase (AST), gamma glutamyltransferase (GG1) and dosage of total, direct and inditect bilirrubin in sorology .The evaluation of the proteinogram demonstrated the inf1uence of the breed factor: the values of total protein in Jersey (6.37±0.90 g/dl) was lower than in Holstein (6.82±0.97g/dl). The analysis of proteins showed the differences between the Jersey and the Holstein animals resulting of the globulins concentrations presents in serum, the values of globulins in Jersey (3.13±0..81g/dl) were lower than in Holstein (3.73±0.99g/dl). Values of alpha-, beta- and gamma-globulins were obtained through the eletrophoresis. In Jersey the values were lower (0.89±0,14g/dl; 0.71±0.14g/dl, 2.03±0.38g/dl) than in Holstein (1.00±0.16g/dl; 0.76±0.15g/dl; 2.20±0.45g/dl). The evaluation of breed factor inf1uence on the AST and GGT seric enzimatic activity showed this inf1uence in AST, but not in GGT. Values of AST in Jersey (49.27±17.87U/l) were higher than in Holstein (34.76±10.61U/l). Values of GGT did not show differences between the Jersey (19.46±33.11U/l) and Holstein (19.08±33.26U/l). The evaluation of seric bilirrubins concentrations showed differences on the total and indirect bilirrubin. Hygher values of indirect bilirrubin (0.40±0.33mg/dl; total bilirrubin 0.44±0.33mg/dl) were seen in Jersey than in Holstein.