ABSTRACT Climate-smart agriculture (CSA) practices, mainly no-tillage (NT), cover cropping (CC), soil fertilization with organic amendments (OA), and crop-livestock (CL) and crop-livestock-forestry (CLF) systems, has been widely adopted in areas from Brazilian Cerrado. The CSA may partly offset former soil C losses and contribute to climate change mitigation. However, contradictory findings brought uncertainties about the effect of CSA on soil C. Here, by a systematic review of 87 papers and using 621 data pairs, we provided a pervasive biome-scale analysis of soil C stock changes associated with the adoption of CSA across Brazilian Cerrado. All CSA practices evaluated showed average positive rates of C stock change, indicating a general tendency of soil C accretion after its adoption. In areas under NT, CC and CLF, greater rates were estimated for the deeper soil profile evaluated (0.00-1.00 m) (1.24 ± 0.85, 0.54 ± 0.54 and 1.00 ± 1.47 Mg ha–1 yr–1, respectively), while OA and CL showed more soil C accretion when the assessment was limited down to 0.10 m depth (0.82 ± 0.60 and 0.59 ± 0.66 Mg ha–1 yr–1, respectively). Unfortunately, the lack of basic information precluded any attempt to statically compare our estimations. In this sense, we must be cautious in stating that soil C sequestration occurs at those rates after the adoption of CSA practices. Despite these limitations, the results clearly show that the diversification and intensification of agricultural areas in the Cerrado by the adoption of CSA is a promising pathway to increase soil C stocks, and consequently, contribute to climate change mitigation and adaptation. Finally, our findings emphasize the importance of efforts that stimulate farmers to adopt these practices on large scale, such as Brazil’s Low-Carbon Agriculture Plan, besides providing sound empirical evidence about the role of soil C sequestration in Brazil achieving its Nationally Determined Contributions commitments.

ABSTRACT Agrosilvopastoral systems have been used as sustainable production models that can promote soil organic carbon (SOC) storage. However, there are no simulation studies with the Century model to estimate the SOC accumulation capacity in the long term, analyzing the effects of management and climate change in integrated crop-livestock-forest (ICLF) systems. This study aimed to simulate soil C dynamics in two chronosequences of land-use composed of native vegetation (NV), degraded pasture (DPA) and ICLF system in the Cerrado of Minas Gerais, in addition to designing future scenarios to verify the potential of SOC accumulation through climate change. The results showed that the Century model reliably simulated the SOC stocks in the two chronosequences evaluated. The model predicted an increase in SOC stocks at two sites by converting the DPA system (46.04 and 42.38 Mg ha-1) into ICLF systems (54.94 and 51.71 Mg ha-1). The Century also predicted that a 20 mm decrease in rainfall and a 2 °C increase in temperature in the tropical regions studied could reduce the SOC stocks more expressively in degraded pastures, while agroforestry systems could show a smaller reduction in SOC stocks. In addition, the results showed that replacing degraded pastures into agrosilvopastoral systems, especially in clayey soils, contributes to increasing SOC stocks. Thus, agroforestry systems are potentially viable to maintain the sustainability of agriculture in the face of climate change.

ABSTRACT To meet the growing demand for bioenergy such as ethanol, the area cultivated with sugarcane has expanded, especially in areas currently occupied by extensive pastures with low productivity. However, land-use change (LUC) from pasture to sugarcane implies changes in soil structure and variations in organic matter (SOM) stored in the soil. This study aimed to quantify the impact of LUC on organic matter fractions – particulate organic matter (POM) and mineral-associated organic matter (MAOM) – in soils with contrasting textures, and to explore the correlations between possible alterations in soil aggregation and the effects on carbon (C) stocks and SOM fractions. The study was conducted in two areas in Central-Southern Brazil, one with clayey soil and the other with sandy soil. In each area, a LUC chronosequence was evaluated: native vegetation (NV), pasture (PA), short-term sugarcane (SC1), the area analyzed during the sugarcane plant cycle; long-term sugarcane (SC2), area analyzed during the ratoon sugarcane cycle. In each use, undisturbed and disturbed samples were collected and macroaggregates and microaggregates were obtained by wet sieving and soil samples. In these samples, the physical fractionation of the SOM and the calculation of the C contained in each fraction of the SOM and C total stock of each use were performed. The conversion of NV to PA increased C stocks by more than 50 %, mainly in the MAOM fraction, and maintenance of macroaggregates (more than 80 %) in sandy site; and reduction of C stocks by more than 30 %, mainly MAOM in the clayey area. These benefits acquired from grazing on sandy area were lost with the expansion of sugarcane, a reduction of more than 20 % in macroagregation and C stocks. The sugarcane expansion into pasture with clayey site resulted in C accumulation (more than 2 Mg C ha-1 yr-1) and recovery of stocks on a SC2 basis. Expanding sugarcane areas into low-productivity pasture areas can be considered a sustainable strategy, especially in clayey soils, in which multiple gains occur through C sequestration and improved soil quality, as well as the reduction of CO2 emissions through the diversification of the energy matrix with the production of ethanol.

ABSTRACT Soils are the largest terrestrial carbon (C) reservoir, and most of this C is retained as soil organic matter (SOM). Due to its ability to capture, stabilize, and store C for extended periods, soils are considered important allies in decarbonizing the atmosphere. The term ‘C stabilization’ includes a series of mechanisms or processes by which soil C is protected within soils and its losses are reduced through microbial decomposition or leaching. Due to their relevance in the global C cycle, C stabilization mechanisms have received intensive attention from the scientific community. As new analytic technologies push the boundaries of what was previously possible to know, new paradigms emerge. This literature review summarizes the current knowledge of the main mechanisms that may promote SOM stabilization. Factors that govern accumulation of SOM are also addressed. We highlight the role of organo-mineral associations and spatial inaccessibility of SOM due to occlusion within soil aggregates to understand the relative contribution of these mechanisms in different soil conditions (e.g., soil texture, mineralogy, and land- use). In addition, the contribution of cutting-edge approaches and analytical techniques to advance the understanding of SOM protection is presented. Modern techniques to evaluate SOM on a micro, nano, and molecular scale can contribute to the mechanistic understanding of SOM stabilization and the study and adoption of management strategies that maintain and increase C stocks in soils.

ABSTRACT: Reducing soil degradation and its impacts on the environment have been one of the main challenges of the 21st century, exacerbated by a direct link between increases in the human population and soil degradation that raises current and future food security concerns. Despite this, experiences worldwide reveal that degraded land restoration projects have either achieved little success or failed. Thus, understanding the underlying causes and devising appropriate restoration mechanisms is crucial. Soil amelioration using beneficial microorganisms, particularly arbuscular mycorrhizal fungi (AMF), is essential and pragmatic. Glomalin, a type of glycoprotein produced by arbuscular mycorrhizal fungi in the phylum Glomeromycota, contributes to the mitigation of soil degradation. Moreover, AMF and glomalin are highly correlated with other soil physico-chemical parameters and are sensitive to changes in the environment. As a result of this, they have been recommended for monitoring the recovery of degraded soil or stages of soil degradation. In this review, we discuss the role of AMF and glomalin in the restoration of degraded soils, including improvements to the soil structure and soil organic matter (SOM), microbial activity, reduction of fertility loss, bioremediation, and mitigation of the effects of drought and saline stress. We highlight the research gaps and discuss the prospects. This knowledge will improve our understanding of the ecological conduct of glomalin and AMF, stimulate future research, and be useful to sustainable restoration of degraded lands. Furthermore, we discussed the challenges and obstacles in the legislation and future perspectives on the production of inoculants based on AMF in Brazil.

ABSTRACT Based on previous reports, our study aimed to obtain the first estimate on the contribution of termite mounds to CH4 emissions in Brazilian Cerrado pastures. We estimated that termite mounds occupy an area larger than 200,000 ha in degraded pastures, an important loss of grazing area considering the current scenario of land-use change of pastures to other crops in Brazil. Moreover, mound-building termites in degraded pastures may be responsible for CH4 emissions greater than 11 Mt CO2 eq. yr−1, which would notably affect the greenhouse gases (GHG) balance of grass-fed cattle production in Brazil. In this sense, it is urgent to conduct field-scale studies about the CH4 emissions by mound-building termites in pastures and its contribution to the C footprint of Brazilian beef.

ABSTRACT: In tropical regions, climate conditions favor fast decomposition of soil organic matter (SOM), releasing into the soil organic composts in solid, liquid, and gaseous forms with variable compositions. Dissolved organic matter (DOM), a complex mixture of thousands of organic compounds, is only a small fraction of the decomposition products; however, it is highly mobile and reactive to the soil. Therefore, DOM play a key role in soil aggregation (formation of organometallic complexes), energy source for microorganisms, as well as C storage, cycling, and provision of plant-available nutrients. DOM multifunctionality to sustain soil functions and important ecosystem services have raised global scientific interest in studies on DOM fractions. However, previous studies were conducted predominantly under temperate soil conditions in natural ecosystems. Therefore, there is paucity of information on tropical soil conditions under agricultural systems, where DOM turnover is intensified by management practices. This review synthesized information in the literature to identify and discuss the main sources, transformations, and future of DOM in soils. We also discussed the importance of this fraction in C cycling and other soil properties and processes, emphasizing agricultural systems in tropical soils. Gaps and opportunities were identified to guide future studies on DOM in tropical soils.

ABSTRACT: The use of crop residues as a bioenergy feedstock is considered a potential strategy to mitigate greenhouse gas (GHG) emissions. However, indiscriminate harvesting of crop residues can induce deleterious effects on soil functioning, plant growth and other ecosystem services. Here, we have summarized the information available in the literature to identify and discuss the main trade-offs and synergisms involved in crop residue management for bioenergy production. The data consistently showed that crop residue harvest and the consequent lower input of organic matter into the soil led to C storage depletions over time, reducing cycling, supply and availability of soil nutrients, directly affecting the soil biota. Although the biota regulates key functions in the soil, crop residue can also cause proliferation of some important agricultural pests. In addition, crop residues act as physical barriers that protect the soil against raindrop impact and temperature variations. Therefore, intensive crop residue harvest can cause soil structure degradation, leading to soil compaction and increased risks of erosion. With regard to GHG emissions, there is no consensus about the potential impact of management of crop residue harvest. In general, residue harvest decreases CO2 and N2O emissions from the decomposition process, but it has no significant effect on CH4 emissions. Plant growth responses to soil and microclimate changes due to crop residue harvest are site and crop specific. Adoption of the best management practices can mitigate the adverse impacts of crop residue harvest. Longterm experiments within strategic production regions are essential to understand and monitor the impact of integrated agricultural systems and propose customized solutions for sustainable crop residue management in each region or landscape. Furthermore, private and public investments/cooperations are necessary for a better understanding of the potential environmental, economic and social implications of crop residue use for bioenergy production.

ABSTRACT: Soil organic matter (SOM), which influences chemical, physical and biological soil attributes, is the main form of C found in the soil which can also be used as a soil sustainability index. The aim of this study was to use the quantity and quality of SOM as an indicator to determine the sustainability of different land uses (native vegetation, secondary vegetation, fruit orchards, horticultural areas, degraded pasture, improved pasture, and fields with annual crops) in the eastern Amazon. Improved pasture had higher soil C stock than the other land uses and was similar to the native vegetation, and also presented the highest quantity of C in a stable form in the soil (fraction < 53 μm). According to the C management index, improved pasture is the most similar in use to native vegetation. Changes in land use reduced the soil microbial C content, although the more conservationist systems (fruit orchards, secondary vegetation, and improved pasture) had contents similar to those of the native vegetation. The highest soil microbial quotients were found in fruit orchards and horticultural areas. Well-managed pastures were effective in accumulating C as stable forms in the soil, which demonstrates the sustainability of this land use in the region studied.

The knowledge about the architecture of sugarcane root system is limited due to difficulties associated to methodological aspects. The correct way to assess the root system is still not clearly established. This study aimed to compare two direct methods of root biomass evaluation: soil monoliths and root sampling from probes. Three experimental plots (7.2 m²) were randomly established in an area under sugarcane planting on Typic Quartzipsamments located in São Pedro, Sao Paulo state, Brazil. One plant in each experimental plot was selected for root system assessment. The sampling with probes was performed in nine points inside half of the Voronoi polygon and the root density was estimated in nine combinations of sampling points. The root samples collected by the two methods were separate from soil particles by washing in a set of sieves. Afterwards, the roots were oven-dried and weighed. Significant differences(p<0.05) in the density root were found among the soil layers in each treatment. A trend of decreasing root density with soil depth was found in both methods. The probes method tended to underestimate the amount of root found in the soil top layer. Only two sampling combinations with probe indicated to be equivalent to the monolith method.

O conhecimento da arquitetura do sistema radicular da cana-de-açúcar ainda è limitado devido às dificuldades metodológicas para o seu estudo. A forma correta de avaliar o sistema radicular não foi claramente estabelecida. Este estudo objetivou a comparação de dois mètodos diretos de avaliação da biomassa radicular: coleta de monólitos de solo e coleta de amostras em sonda. Para isto foram estabelecidas três parcelas experimentais (7,2 m²) completamente ao acaso em área de plantio de cana-de-açúcar sobre solo Neossolo Quartzarenico localizado no município de São Pedro - SP. Uma planta dentro de cada parcela experimental foi selecionada para o estudo do sistema radicular. A amostragem com sonda foi realizada em 9 pontos dentro da metade do polígono de voronoi e a densidade radicular estimada em 9 combinações de pontos de amostragem. As amostras de raízes coletadas pelos dois mètodos de estudo foram separadas do solo por meio da lavagem com água corrente em um conjunto de peneiras. Posteriormente as raízes foram colocadas para secar e finalmente pesadas em balança analítica. Diferenças significativas (p<0.05) foram encontradas para os resultados da densidade radicular entre as camadas avaliadas para cada tratamento. Tanto o mètodo monólito quanto o mètodo da sonda indicaram tendência de decrèscimo da biomassa radicular com a profundidade. De forma geral o mètodo da sonda tendeu a subestimar a quantidade de raiz encontrada na camada superficial. Somente duas combinações de amostragem com sonda mostraram ser equiparáveis ao mètodo do monólito.

ABSTRACT The substitution of native vegetation by agricultural ecosystems can change the quantity and quality of soil organic matter (SOM), and the intensity of these changes depends on the soil type, climate, and land use. The objective of this study was to evaluate the quality of organic matter in chronosequences of Jatropha cultivation in contrasting soil and climatic conditions. Soil samples were evaluated at depths of 0.00-0.10, 0.20-0.30, and 0.80-1.00 m in chronosequences located in Planaltina, Distrito Federal (Cerrado-Pasture-Jatropha), Dourados, Mato Grosso do Sul (Atlantic Forest-Corn-Jatropha), and Araripina, Pernambuco (Caatinga-Jatropha). To assess SOM quality, we determined C contents in the SOM fractions, C stocks, the carbon management index (CMI), the SOM humification index (HLIF), and the C and N concentrations in the microbial biomass. The conversion of native vegetation to agropastoral systems changed the composition of SOM in the biomes evaluated, especially in the surface layers. The CMI and the C and N contents in the microbial biomass were the most responsive to land use changes in all the biomes studied. The pasture improved SOM quality by increasing the CMI (116) and the C content by 8, 21, and 6 % in the organic, mineral, and organomineral fractions, respectively, while maintaining the SOM humification index and the C and N contents in the microbial biomass in the 0-0.10 m layer. The lowest values of C in the SOM fractions, the CMI (52), and C microbial biomass (136 mg kg-1) were observed for annual crops. Jatropha cultivation increased C contents in the SOM fractions, C stocks, the CMI, and C and N in the microbial biomass with an increase in cultivation time, which demonstrates the potential of this long-term system for improving SOM quality.

Resumo O objetivo deste trabalho foi avaliar atributos químicos do solo, quantificar os teores e os estoques de C e N, e avaliar a qualidade química da matéria orgânica do solo em sistemas de colheita de cana-de-açúcar com ou sem queima da palhada. Áreas cultivadas com cana-de-açúcar foram avaliadas com ou sem queima da palhada, aos 6 e aos 12 anos após a última reforma do canavial. Além disso, também foram avaliadas uma área de vegetação nativa e outra cultivada por 19 anos sem queima. O teor de C no solo das áreas sem queima foi superior ao daquelas queimadas e inferior ao da área com vegetação nativa. O estoque de C a 0,0-0,3 m de profundidade, na área queimada, foi 22% menor do que o na área sem queima, aos 6 anos, e 43% menor, aos 12 anos. Os maiores graus de humificação ocorreram na área sem queima por 19 anos; e os menores, na área com vegetação nativa. Os solos cultivados apresentaram maior concentração de grupos fenólicos e carboxílicos nos ácidos húmicos. A fertilidade do solo aumenta em áreas de cana-de-açúcar sem queima, em razão da matéria orgânica do solo mais humificada e da maior quantidade de grupamentos carboxílicos e fenólicos.

Abstract The objective of this work was to evaluate soil chemical attributes, to quantify soil contents and stocks of C and N, and to evaluate the chemical quality of soil organic matter in sugarcane harvest systems with or without burning. Cultivated sugarcane areas were evaluated with or without burning of the fields at 6 and 12 years after the last renovation of the sugarcane fields. In addition, a native vegetation area and another one cultivated for 19 years without burning were also evaluated. Soil C content in the unburnt areas was higher than that of the burnt ones and lower than that of the areas with native vegetation. Carbon stock at the 0.0-0.3-m soil depth in the burnt areas was 22% lower than in the 6-year unburnt area and 43% lower in the 12-year area. The highest humification degrees occurred in the 19-year unburnt area; and the lowest, in the native vegetation area. The cultivated soils showed higher concentration of phenolic and carboxylic groups in humic acids. Soil fertility increases in the unburnt sugarcane areas because of their more humified organic matter and of the higher quantity of carboxylic and phenolic groups.

RESUMO A demanda crescente de alimentos gera problemas ambientais, principalmente devido à remoção das coberturas vegetais nativas para a expansão da agricultura no Brasil. Essas alterações no uso da terra acarretam mudanças na dinâmica da matéria orgânica do solo. Os micro-organismos representam a maior diversidade biológica e fisiológica do solo, além de serem responsáveis por mais de 95% dos processos de decomposição e ciclagem de nutrientes. O objetivo deste trabalho foi verificar se há diferenciação dos padrões de atividade da biomassa microbiana dos solos sob as diferentes vegetações naturais, pastagens em uso e sistemas agrícolas recém-implantados. A área de abrangência desta pesquisa corresponde aos Estados de Rondônia e Mato Grosso. Foi realizada a análise de variável canônica de atributos físicos, químicos e microbiológicos de cada ecorregião e uso da terra, buscando evidenciar os padrões e as variáveis que os diferenciam. As áreas nativas apresentaram padrões distintos na dinâmica dos atributos microbiológicos relacionados principalmente à quantidade de serapilheira em cada bioma estudado. Em relação às áreas antropizadas, observaram-se resultados similares entre pastagens e áreas nativas, significativamente diferentes dos resultados obtidos em áreas agrícolas, que, devido à diferença de manejo e diversidade de culturas analisadas, apresentaram uma grande variabilidade no seu resultado final. Os resultados obtidos mostram que os atributos microbiológicos devem ser utilizados como indicadores de mudança de uso da terra quando associados a fatores químicos e físicos do solo.

ABSTRACT The increasing demand for food creates environmental problems, mainly due to the removal of native vegetation cover for agriculture expansion in Brazil. These changes in land use lead to changes in the soil organic matter dynamics. Microorganisms represent the most biological and physiological diversity in soil, as well as are responsible for more than 95% of the decomposition and nutrient cycling processes. The objective in this research was to check if there is difference of patterns in activity of soil microbial biomass under varied natural vegetation, pastures in use and agricultural systems recently established. The area covered by this study corresponds to the states of Rondônia and Mato Grosso. Canonical variate analysis was used in physical, chemical and microbiological factors in each ecoregion and land use, looking for patterns and variables that can differentiate them. The native areas showed distinct patterns in the dynamics of microbiological attributes mainly related to the amount of litter in each biome studied. For the disturbed areas, there were similar results between pastures and native areas, significantly different from the results obtained in agricultural areas, which, due to differences in management and kind of cultures analyzed, showed a great variability in the final result. The results support the recommendation for use of microbiological attributes as indicators of land use change, combined with chemical and physical factors of the soil.

Abstract Land use changes strongly impact soil functions, particularly microbial biomass diversity and activity. We hypothesized that the catabolic respiration response of the microbial biomass would differ depending on land use and that these differences would be consistent at the landscape scale. In the present study, we analyzed the catabolic response profile of the soil microbial biomass through substrate-induced respiration in different land uses over a wide geographical range in Mato Grosso and Rondônia state (Southwest Amazon region). We analyzed the differences among native areas, pastures and crop areas and within each land use and examined only native areas (Forest, Dense Cerrado and Cerrado), pastures (Nominal, Degraded and Improved) and crop areas (Perennial, No-Tillage, Conventional Tillage). The metabolic profile of the microbial biomass was accessed using substrate-induced respiration. Pasture soils showed significant responses to amino acids and carboxylic acids, whereas native areas showed higher responses to malonic acid, malic acid and succinic acid. Within each land use category, the catabolic responses showed similar patterns in both large general comparisons (native area, pasture and crop areas) and more specific comparisons (biomes, pastures and crop types). The results showed that the catabolic responses of the microbial biomass are highly correlated with land use, independent of soil type or climate. The substrate induced respiration approach is useful to discriminate microbial communities, even on a large scale.

A cana-de-açúcar é a principal cultura utilizada na produção de etanol biocombustível no Brasil e sua colheita pode ser feita com ou sem queima das folhas, aumentando ou diminuindo a emissão de gases do efeito estufa e a deposição de C no solo. Por meio deste trabalho, avaliou-se o efeito de sistemas de colheita de cana-de-açúcar (com e sem queima da palha, com um, três e seis anos após a última reforma do canavial) sobre os teores e estoques de C no solo, a qualidade física da matéria orgânica e a imobilização de C na biomassa microbiana do solo. As áreas de colheita sem queima apresentaram maior teor de C na camada superficial e maiores estoques de C, independentemente do tempo após a última reforma. Diferenças na qualidade física da matéria orgânica ocorreram principalmente na fração com tamanho entre 53 e 75 µm, na qual a proporção de C foi maior nas áreas sem queima. Na camada de 0-10 cm, o sistema sem queima apresentou maior teor de C microbiano. A colheita sem queima da palha é eficiente em acumular C em formas que possuem alto tempo de residência no solo.

Sugarcane is the main crop used in ethanol biofuel production in Brazil and it may be harvested with or without burning, increasing or decreasing greenhouse gases emissions and soil carbon deposition. In this study, we evaluated the effect of the sugarcane harvest system (with and without burning, at one, three, and six years after the last replanting) on C contents and C stocks in the soil, the physical quality of soil organic matter, and C immobilization in soil microbial biomass. The areas harvested without burning showed higher C content in the surface layer and higher C stocks, regardless of the time after the last replanting. Differences in the physical quality of organic matter mainly occurred in the 53 to 75 µm particle size fraction, in which the C ratio was higher in the unburned areas. In the 0-10 cm layer, harvesting without burning was associated with higher microbial C. Harvest without straw burning is effective in accumulating C in forms with long residence time in the soil.