Abstract Saccharomyces cerevisiae (S. cerevisiae) has been studied as a model microorganism to understand the mechanism of glutathione (GSH) production. Furthermore, magnetic field (MF) application is a novel approach to stimulate microorganism metabolism to increase growth and produce more biomolecules. This study aimed to evaluate whether GSH production by S. cerevisiae ATCC 7754 would be affected by different pressure levels and MF application in an airlift bioreactor. Influence of pressure (0.0 - 0.15 MPa), medium recycle velocity (3.0 and 15.0 cm s-1) and MF application (0.0 and 3.0 mT) was evaluated. Biomass and GSH concentrations at high pressure and medium recycle velocity were higher than the ones in the control, whereas MF decreased biomass and GSH concentrations. The best results of biomass concentration and GSH production were 17.79 g L-1 and 162.92 mg L-1, respectively. at 0.05 MPa, 15.0 cm s- 1 and without any MF application. Results of the pressured airlift bioreactor showed that this kind of reactor may be used for producing GSH and for reducing energy costs.

Resumo O objetivo deste trabalho foi avaliar a potencial utilização do bagaço de uva seco (FBU) obtido a partir das operações de secagem e moagem, juntamente com o farelo de arroz parboilizado (FAP), para a elaboração de biscoitos. Os subprodutos foram caracterizados com relação ao conteúdo de umidade, proteínas, lipídeos, carboidratos, fibras e cinzas, além das análises de índice de absorção, solubilidade em água e acidez. As formulações propostas foram elaboradas variando a quantidade dos subprodutos em substituição à farinha de trigo. Os biscoitos elaborados foram submetidos à análise microbiológica e à análise sensorial. A formulação com maior aceitação a partir da análise sensorial foi a que continha 5% de farinha do bagaço de uva e 10% de farelo de arroz. A caracterização físico-química do biscoito com maior aceitação teve como destaque os teores de proteínas e fibras, que apresentaram 5,15% e 28,42%, respectivamente. A partir dos resultados obtidos, o presente estudo apresenta um produto adequado para o consumo tanto do ponto de vista microbiológico quanto do nutricional.

Abstract The aim of this study was to evaluate the potential use of dry grape marc obtained of drying and milling operations with parboiled rice bran for cookies production. By-products were characterized with respect to moisture content, proteins, lipids, carbohydrates, fiber, and ashes, besides the analysis of absorption index, solubility in water and acidity. The proposed formulations were prepared by varying the amount of by-products to replace wheat flour. Elaborate cookies were subjected to microbiological and sensory analysis. The formulation with greater acceptance contained 5% of flour grape marc and 10% of parboiled rice bran. The physicochemical characterization of the cookies with greater acceptance was highlighted by the protein and fiber that presented 5.15% and 28.42%, respectively. The result obtained from this study shows a suitable product for consumption for microbiological as well as nutritional point of view.