In this work, a search for antimicrobial agents against multi-drug resistant (MDR) bacteria was undertaken. It involved two natural diterpenes of different basic skeletons, named ent-kaurenoic acid and ent-pimaradienoic acid, that were used as precursors to access 28 semi-synthetic derivatives that were also submitted to biological assays. All 30 substances were assayed against a set of seven clinically isolated MDR bacteria, including three Staphylococcus aureus strains. Results classified both natural compounds as promising bactericidal antimicrobial agents against these MDR bacteria, especially for their performance in time-kill curve assay, improving on the positive control vancomycin. Moreover, di-hydro ent-pimaradienoic acid, obtained as one of the derivatives, also displayed promising minimum inhibitory concentration (MIC) values against the microorganism set, proving optimum activity against S. aureus strains. Thereby, this work also yielded a promising semi-synthetic bacteriostatic antimicrobial agent against MDR S. aureus. Furthermore, the study was carried out with 30 structures in order to verify the veracity and scope of Urzúa’s hypothesis about the structure-activity relationship between diterpenes and antimicrobial action. Our results are 93% in agreement with this hypothesis.

The labdane diterpenes copalic acid, 3β-acetoxy-copalic acid, 3β-hydroxy-copalic acid and ent-agathic acid were isolated from Copaifera langsdorffii oleoresin. These four compounds were submitted to structural modifications by reduction with hydrogen/palladium, esterification with diazomethane, esterification with methanol/sulfuric acid and conversion into sodium salt, furnishing 15 compounds. All compounds were assayed in vitro against Mycobacterium tuberculosis (H37Rv, ATCC 27294). The four compounds displayed minimum inhibitory concentration (MIC) value of 125 µg mL-1, and were not considered active. A methylated derivative of compound 3β-hydroxy-copalic acid, and a sodium salt of copalic acid displayed MIC values of 25 µg mL-1 (71.7 µM) and 6.25 µg mL-1 (19.2 µM), respectively. The sodium salt of copalic acid stood out by displaying similar activity in comparison with streptomycin (MIC 6.25 µg mL-1) and a better activity compared to µM value of pyrazinamide (MIC 3.12 µg mL-1; 25.34 µM). Therefore, the methylated derivative of compound 3β-hydroxy-copalic acid and the sodium salt of copalic acid should be considered for further studies.

Abstract Residual stresses are present in materials or structural component in the absence of external loads or changes in temperatures. The most common causes of residual stresses being present are the manufacturing or assembling processes. All manufacturing processes, such as casting, welding, machining, moulding, heat treatment, etc, introduces residual stresses into the manufactured object. The residual stresses effects could be beneficial or detrimental, depending on its distribution related to the component or structure, its load service and if they are compressive or tensile. In order to do the studies of residual stresses inside pipes, where the fatigue cracks normally initiate, an equipment that allows applying the Hole-Drilling Method was developed. The equipment efficacy was confirmed in this work by using it to detect residual strains inside a Mock-up that simulates the relief and security nozzle of Angra 1 Nuclear Power Plant (NPP) pressuriser.

ABSTRACT Insulin-like Growth Factor-1 (IGF-1) gene polymorphism has been associated with an increased risk for breast cancer. IGF-1 is a key regulator of proliferation, cell differentiation and apoptosis. It has important mitogenic and anti-apoptotic activities in normal cells and in breast cancer cells, acting synergistically with estrogen to increase neoplastic cell proliferation. This review aims to present the recent finds of IGF-1 gene polymorphism and its relationship with the risk of breast cancer through following the polymorphic dinucleotide repeat cytosine-adenine (CA) and single nucleotide polymorphisms (SNPs) by searching in the PubMed database publications focused studies published from 2010 to 2015 related to IGF-1 gene polymorphism and breast cancer risk. A growing number of studies support an association between IGF-1 gene polymorphism and breast cancer risk with conflicting results, nevertheless elucidation of the patterns of IGF-1 gene expression may permit characterization of women at high-risk for breast cancer, as well as the development of strategies for early diagnosis and efficient treatment against the disease.

In this work we present a complete proton (1H) and carbon 13 (13C) nuclear magnetic resonance (NMR) spectral analysis of two synthetic dihydrofuran neolignans (±)-trans-dehydrodicoumarate dimethyl ester and (±)-trans-dehydrodiferulate dimethyl ester. Unequivocal assignments were achieved by 1H NMR, proton decoupled 13C (13C{1H}) NMR spectra, gradient-selected correlation spectroscopy (gCOSY), J-resolved, gradient-selected heteronuclear multiple quantum coherence (gHMQC), gradient-selected heteronuclear multiple bond coherence (gHMBC) and nuclear Overhauser effect spectroscopy (NOESY) experiments. All hydrogen coupling constants were measured, clarifying all the hydrogen signals multiplicities. Computational methods were also used to simulate the 1H and 13C chemical shifts and showed good agreement with the trans configuration of the substituents at C7 and C8.

The inconveniences of the conventional method for biodiesel production by alkaline catalysis suggests research towards alternative methods, with the non-catalytic transesterification using an alcohol at supercritical conditions proposed as a promising technique for biodiesel production. The so-called supercritical method (SCM) has powerful advantages over conventional techniques, such as fast reaction rates, feedstock flexibility, production efficiency and environmentally friendly benefits. However, application of this methodology has some limitations, like operating conditions (elevated temperature and pressure and higher amounts of alcohol), which result in high energy costs and degradation of the products generated. In this review paper the state of the art in relation to the use of the SCM for biodiesel production is reported and discussed, describing the characteristics of the method, the influence of operational parameters on the ester yield, patents available in the field and the perspectives for application of the technique.

A frequência de trovoadas a partir de dados de satélite TRMM e do DECEA foi estudada para o período de 10 anos (de 1998 até 2007), onde se observou que as trovoadas foram mais frequentes nos meses de verão, com maior ocorrência no mês de março. Em anos de El Niño ocorre uma diminuição de cerca de 20% dos casos de trovoadas em Alagoas, mas nos anos normais e de La Niña observa-se um aumento dos dias de trovoadas de até 80%. As trovoadas foram relacionadas, em baixos níveis, aos Alísios com curvatura ciclônica e em altos níveis à presença de VCAN (Vórtice Ciclônico em Altos Níveis), CJNEB (Corrente de Jato no Nordeste do Brasil) e Anticiclone sobre o Estado de Alagoas. As trovoadas se formaram predominantemente a noroeste do VCAN e na região da entrada (lado quente) e na saída (lado frio) da CJNEB. As análises termodinâmicas mostraram que as curvas de temperatura do ar e de temperatura do ponto de orvalho observadas foram semelhantes às curvas previstas, construídas pelas trajetórias das parcelas de ar pelo modelo HYSPLIT, nas previsões até 48 e 24h de antecedência, respectivamente. Os índices LI e CAPE+ previram uma atmosfera muito instável com 48h de antecedência.

The thunderstorm frequency was studied using TRMM satellite and the DECEA data for the 10 year period (from 1998 to 2007). It was observed that the storms were more frequent in the summer months, with greater occurrence in March. The El Nino event inhibits the phenomenon (only 20% of cases of all), but in the La Niña and normal years, was observed an increase in days of storms up to 80% of the cases. The thunderstorms were associated with trade winds, with cyclonic curvature and in the high levels with ULCV, JSNEB and anticyclone. The thunderstorms were formed predominantly in northwestern ULCV in the entrance (warm side) and in the exit (cold side) of JSNEB. The observed vertical profiles of air and the dew point temperatures were similar to the forecasted profiles, constructed by the air parcels trajectories by HYSPLIT model, up to 48h and up to 24h antecedently, respectively. The index LI and CAPE+ predicted a very unstable atmosphere with 48 hours antecedence.