ABSTRACT BACKGROUND: Computer-aided diagnosis in low-dose (≤ 3 mSv) computed tomography (CT) is a potential screening tool for lung nodules, with quality interpretation and less inter-observer variability among readers. Therefore, we aimed to determine the screening potential of CT using a radiation dose that does not exceed 2 mSv. OBJECTIVE: We aimed to compare the diagnostic parameters of low-dose (< 2 mSv) CT interpretation results using a computer-aided diagnosis system for lung cancer screening with those of a conventional reading system used by radiologists. DESIGN AND SETTING: We conducted a comparative study of chest CT images for lung cancer screening at three private institutions. METHODS: A database of low-dose (< 2 mSv) chest CT images of patients at risk of lung cancer was viewed with the conventional reading system (301 patients and 226 nodules) or computer-aided diagnosis system without any subsequent radiologist review (944 patients and 1,048 nodules). RESULTS: The numbers of detected and solid nodules per patient (both P < 0.0001) were higher using the computer-aided diagnosis system than those using the conventional reading system. The nodule size was reported as the maximum size in any plane in the computer-aided diagnosis system. Higher numbers of patients (102 [11%] versus 20 [7%], P = 0.0345) and nodules (154 [15%] versus 17 [8%], P = 0.0035) were diagnosed with cancer using the computer-aided diagnosis system. CONCLUSIONS: The computer-aided diagnosis system facilitates the diagnosis of cancerous nodules, especially solid nodules, in low-dose (< 2 mSv) CT among patients at risk for lung cancer.

ABSTRACT Introduction: The aerobic endurance training of sports athletes has become the focus of research in institutions and related units. One of the recent proposals is high-intensity interval training to develop athletic aerobic endurance. Objective: Study the effect of high-intensity interval training on aerobic endurance in basketball players. Methods: Fifteen athletes from a university were randomly selected and divided into a high-intensity interval training group, a moderate-intensity continuous training group, and a low-intensity continuous training group, with the control of external influence factors. The indicators analyzed were the average aerobic endurance index of maximal oxygen consumption, average anaerobic capacity, and average maximal power output. The data were obtained before and after the experiment. They were compared and discussed using statistical analysis. Results: The average maximal oxygen consumption in the three training groups increased significantly, and the effect was most evident under the high-intensity intermittent training conditions. This training method could increase the maximal exercise time of the normal groups by up to two times. Conclusion: Intermittent high-intensity training can effectively improve the aerobic endurance of basketball players. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción: El entrenamiento de la resistencia aeróbica de los atletas deportivos se ha convertido en el centro de la investigación en instituciones y unidades relacionadas. Para desarrollar la resistencia aeróbica atlética, una de las propuestas recientes es el entrenamiento de intervalos de alta intensidad. Objetivo: Estudiar el efecto del entrenamiento interválico de alta intensidad sobre la resistencia aeróbica en jugadores de baloncesto. Métodos: Se seleccionaron aleatoriamente quince atletas de una universidad y se dividieron en un grupo de entrenamiento por intervalos de alta intensidad, un grupo de entrenamiento continuo de intensidad moderada y un grupo de entrenamiento continuo de baja intensidad, con el control de los factores de influencia externos. Entre los indicadores analizados estaban el índice medio de resistencia aeróbica de consumo máximo de oxígeno, la capacidad anaeróbica media y la potencia máxima media. Los datos se obtuvieron antes y después del experimento. Se compararon y discutieron mediante un análisis estadístico. Resultados: El consumo medio máximo de oxígeno en los tres grupos de entrenamiento aumentó significativamente, y el efecto fue más evidente en las condiciones de entrenamiento intermitente de alta intensidad. Este método de entrenamiento presentaba el potencial de aumentar el tiempo máximo de ejercicio de los grupos normales hasta dos veces. Conclusión: El entrenamiento intermitente de alta intensidad puede mejorar eficazmente la resistencia aeróbica de los jugadores de baloncesto. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

RESUMO Introdução: O treinamento da resistência aeróbica dos atletas esportivos tornou-se o foco na pesquisa de instituições e unidades relacionadas. No intuito de desenvolver a resistência aeróbica atlética, uma das propostas recentes é o treinamento intervalado de alta intensidade. Objetivo: Estudar o efeito do treinamento intervalado de alta intensidade sobre a resistência aeróbica nos jogadores de basquetebol. Métodos: Foram selecionados aleatoriamente 15 atletas de uma universidade, que foram divididos em um grupo de treinamento de intervalo de alta intensidade, um grupo de treinamento contínuo de intensidade moderada e um grupo de treinamento contínuo de baixa intensidade, com o controle dos fatores de influência externa. Entre os indicadores analisados estão o índice de resistência aeróbica média de consumo máximo de oxigênio, a capacidade anaeróbica média e a potência máxima média. Os dados foram obtidos antes e após o experimento. Foram comparados e discutidos através de análise estatística. Resultados: O consumo máximo médio de oxigênio nos três grupos de treinamento aumentou significativamente, e o efeito foi mais evidente sob as condições de treinamento intermitente de alta intensidade. Este método de treinamento apresentou o potencial de aumentar o tempo máximo de exercício dos grupos normais em até duas vezes. Conclusão: O treinamento intermitente de alta intensidade pode efetivamente melhorar a resistência aeróbica dos jogadores de basquete. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

Although bivalirudin has been recently made available for purchase in China, large-scale analyses on the safety profile of bivalirudin among Chinese patients is lacking. Thus, this study aimed to compare the safety profile of bivalirudin and heparin as anticoagulants in Chinese ST-segment elevation myocardial infarction (STEMI) patients undergoing percutaneous coronary intervention (PCI). A total of 1063 STEMI patients undergoing PCI and receiving bivalirudin (n=424, bivalirudin group) or heparin (n=639, heparin group) as anticoagulants were retrospectively enrolled. The net adverse clinical events (NACEs) within 30 days after PCI were recorded, including major adverse cardiac and cerebral events (MACCEs) and bleeding events (bleeding academic research consortium (BARC) grades 2-5 (BARC 2-5)). The incidences of NACEs (10.1 vs 15.6%) (P=0.010), BARC 2-5 bleeding events (5.2 vs 10.3%) (P=0.003), and BARC grades 3-5 (BARC 3-5) bleeding events (2.1 vs 5.5%) (P=0.007) were lower in the bivalirudin group compared to the heparin group, whereas general MACCEs incidence (8.9 vs 6.4%) (P=0.131) and each category of MACCEs (all P>0.05) did not differ between two groups. Furthermore, the multivariate logistic analyses showed that bivalirudin (vs heparin) was independently correlated with lower risk of NACEs (OR=0.508, P=0.002), BARC 2-5 bleeding events (OR=0.403, P=0.001), and BARC 3-5 bleeding events (OR=0.452, P=0.042); other independent risk factors for NACEs, MACCEs, or BARC bleeding events included history of diabetes mellitus, emergency operation, multiple lesional vessels, stent length >33.0 mm, and higher CRUSADE score (all P<0.05). Thus, bivalirudin presented a better safety profile than heparin among Chinese STEMI patients undergoing PCI.

ABSTRACT Background: With the reduction of global forest resources and the intensification of environmental problems, it is particularly important to seek new solutions for forest resource management. This paper proposes a forest resource management system based on blockchain technology to realize data security sharing and traceability of forest ownership. Through drone on-site verification and blockchain technology, management credibility and efficiency are improved and costs are reduced. The system provides innovative solutions in terms of data credibility, cooperation mechanism, intelligent decision-making, sustainable development, etc., and defines the future development direction. Background problems ownership onsite site reduced mechanism decisionmaking, decisionmaking decision making, making decision-making etc etc. direction

ABSTRACT Tillage is an important management tool for tackling and promoting water conservation and improving crop yield. As one of the important nutrients in plant growth, K is involved in important processes such as osmoregulation, photosynthesis and metabolite transport, and plays a particularly critical role in improving crop yield and quality. In the long-term positioning platform of the tillage method, a 2-yr field experiment was conducted in 2019-2020 in maize (Zea mays L.) Three tillage methods: conventional tillage (CT), subsoil tillage (ST), and no-tillage (NT) and two planting densities 6×104 (D1) and 9×104 plants ha-1 (D2) were set up in the experiment. The results showed that yield and K translocation efficiency (KTE) were significantly higher in NT than in CT at D1 (by 4.7% and 12.2%) and D2 (by 14.0% and 13.9%), respectively. At maturity stage in 2019, population DM accumulation after silking (DMA) was significantly higher in NT (by 11.0% and 16.9%) than in CT at D1 and D2. Correlation analysis revealed that yield was significantly positive correlated with ears (r = 0.57***) and DMA (r = 0.64***). Potassium translocation and K harvest index were positively correlated with KTE. Under spring drought year, the long-term no-tillage had a significant yield increase, mainly through the increase in 1000-kernel weight. The increase in K efficiency was mainly through the influence of DM accumulation and distribution, and K accumulation in grain.

RESUMO Introdução: Pesquisas recentes demonstram que o CORE é o elo central na cadeia cinética da ginástica aeróbica. As apresentações sincronizadas dos atletas dependem não só da força quanto também do equilíbrio e precisão dos movimentos que partem dessa área. Embora reconheça-se a importância desse grupo muscular, não há um consenso sobre o protocolo mais eficaz para aumentar o desempenho em atletas de ginástica aeróbica. Objetivo: Desenvolver um protocolo de treinamento do CORE e verificar sua influência sobre o equilíbrio dos atletas em ginástica aeróbica. Métodos: Este artigo seleciona atletas de ginástica aeróbica de alto nível e os divide aleatoriamente em grupos experimentais e de controle. A experiência foi conduzida em 8 semanas. O grupo experimental adotou métodos sistemáticos de treinamento de força central, e o grupo de controle continuou com os métodos tradicionais de treinamento de força convencional. Os voluntários foram testados quanto à aptidão física antes e depois do experimento. Resultados: A aptidão física e as habilidades atléticas dos atletas de ginástica aeróbica foram significativamente aprimoradas após o treinamento. Conclusão: O protocolo de treinamento do CORE em atletas de ginástica aeróbica elevou a qualidade de execução nos movimentos complexos. Nível de evidência II; Estudos Terapêuticos - Investigação de Resultados.

ABSTRACT Introduction: Recent research shows the CORE is the central link in the kinetic chain of aerobic gymnastics. The athlete’s synchronized performances depend not only on strength but also on the balance and precision of the movements that start from this area. Although the importance of this muscle group is recognized, there is no consensus about the most effective protocol to increase performance in aerobic gymnastics athletes. Objective: Develop and verify the influence of a protocol for CORE training on the aerobic gymnasts’ balance. Methods: This paper selects high-level aerobic gymnastics athletes and randomly divides them into experimental and control groups. The experiment was conducted over eight weeks. The experimental group adopted systematic core strength training methods, and the control group continued with traditional conventional strength training methods. Volunteers were tested for physical fitness before and after the experiment. Results: Aerobic gymnastics athletes’ physical fitness and athletic abilities were significantly improved after training. Conclusion: The CORE training protocol in aerobic gymnastics athletes increased the execution quality in complex movements. Evidence Level II; Therapeutic Studies - Investigating the result.

RESUMEN Introducción: Investigaciones recientes demuestran que el CORE es el eslabón central de la cadena cinética de la gimnasia aeróbica. Las presentaciones sincronizadas de las atletas dependen no sólo de la fuerza sino también del equilibrio y la precisión de los movimientos que parten de esta zona. Aunque se reconoce la importancia de este grupo muscular, no hay consenso sobre el protocolo más eficaz para aumentar el rendimiento de las gimnastas aeróbicas. Objetivo: Desarrollar un protocolo de entrenamiento del CORE y comprobar su influencia en el equilibrio de los atletas en la gimnasia aeróbica. Métodos: Este trabajo selecciona a atletas de gimnasia aeróbica de alto nivel y los divide aleatoriamente en grupos experimental y de control. El experimento se realizó durante 8 semanas. El grupo experimental adoptó métodos sistemáticos de entrenamiento de la fuerza central, y el grupo de control continuó con los métodos tradicionales de entrenamiento de la fuerza convencional. Los voluntarios fueron sometidos a pruebas de aptitud física antes y después del experimento. Resultados: La aptitud física y las capacidades atléticas de los atletas de gimnasia aeróbica mejoraron significativamente después del entrenamiento. Conclusión: El protocolo de entrenamiento CORE en atletas de gimnasia aeróbica elevó la calidad de ejecución en movimientos complejos. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

RESUMO Introdução: A saúde física das crianças e adolescentes está diretamente relacionada à qualidade do treinamento físico escolar. O aprimoramento da eficácia esportiva adulta é resultado de um protocolo conciso com exercícios padronizados. O exercício de pular corda carece de estudos cinemáticos aplicados em crianças e adolescentes. Objetivo: Fornecer uma referência teórica para o treinamento físico de pular corda para crianças e adolescentes. Métodos: Foram recrutadas 8 crianças com idade entre 6 a 8 anos para saltos em frequência de 80 e 120 pulos por minuto controlados por um metrônomo. A técnica foi dividida em três estágios: impulso, suspensão e pouso. O controle no balanço do exoesqueleto articular do joelho foi pesquisado por baropodometria computadorizada com diferenciação de velocidade angular rotacional pela aceleração angular rotacional via filtro de Butterworth, após limpeza de ruídos e multiplicação por fator de amplificação ajustável, devolvidos à entrada de admissão com torque de compensação inercial. Resultados: Inicialmente, a força de impulso é cerca de três vezes a gravidade de cada indivíduo. Esse valor e a aceleração do centro de massa diminuem com o tempo. A força inicial é proporcional a velocidade da sua diminuição durante o impulso, ocorrendo o inverso com a força de pouso. Observa-se uma maior intensidade ao elevar a frequência. Conclusão: A flutuação na curva de força na fase de pouso após 20 pulos indica baixa força muscular em membros inferiores para manter a atividade. Recomenda-se a velocidade de 80 pulos por minuto controlada por música em ambiente lúdico para crianças. Nível de evidência II; Estudos Terapêuticos - Investigação de Resultados.

ABSTRACT Introduction: The physical health of children and adolescents is directly related to school physical training quality. The improvement of adult sports effectiveness results from a concise protocol with standardized exercises. Rope skipping exercise lacks kinematic studies applied to children and adolescents. Objective: Provide a theoretical reference for jump rope exercise training for children and adolescents. Methods: Eight children aged six to eight years old were recruited to jump at a frequency of 80 and 120 jumps per minute controlled by a metronome. The technique was divided into three stages: impulse, suspension, and landing. Control in the knee joint exoskeleton balance was investigated by computerized baropodometry with differentiation of rotational angular velocity by rotational angular acceleration via Butterworth filter. After noise cleaning and multiplication, an adjustable amplification factor returned to the inlet with inertial compensation torque. Results: Initially, the impulsing force is about three times the individual’s gravity. This value and the center of mass acceleration decrease in time. The initial force is proportional to the velocity of its decrease during the impulse, and the opposite occurs with the landing force. Higher intensity is observed when raising the frequency. Conclusion: The fluctuation in the force curve in the landing phase after 20 jumps indicates low muscle strength in lower limbs to maintain activity. A speed of 80 jumps per minute controlled by music in a playful environment is recommended for children. Evidence Level II; Therapeutic Studies – Investigating the results.

RESUMEN Introducción: La salud física de los niños y adolescentes está directamente relacionada con la calidad del entrenamiento físico escolar. La mejora de la eficacia deportiva del adulto es el resultado de un protocolo conciso con ejercicios estandarizados. El ejercicio de salto de cuerda carece de estudios cinemáticos aplicados a niños y adolescentes. Objetivo: Proporcionar una referencia teórica para el entrenamiento de ejercicios de salto de cuerda para niños y adolescentes. Métodos: Ocho niños de entre 6 y 8 años fueron reclutados para saltar a una frecuencia de 80 y 120 saltos por minuto controlados por un metrónomo. La técnica se dividió en tres etapas: impulso, suspensión y aterrizaje. El control sobre el equilibrio del exoesqueleto de la articulación de la rodilla se investigó mediante baropodometría computarizada con diferenciación de la velocidad angular rotacional por la aceleración angular rotacional a través del filtro Butterworth, después de la limpieza del ruido y la multiplicación por el factor de amplificación ajustable, devuelto a la entrada con par de compensación inercial. Resultados: Inicialmente, la fuerza de empuje es aproximadamente tres veces la gravedad de cada individuo. Este valor y la aceleración del centro de masa disminuyen con el tiempo. La fuerza inicial es proporcional a la velocidad de su disminución durante el impulso, y lo contrario ocurre con la fuerza de aterrizaje. Se observa una mayor intensidad al aumentar la frecuencia. Conclusión: La fluctuación de la curva de fuerza en la fase de aterrizaje tras 20 saltos indica una baja fuerza muscular en los miembros inferiores para mantener la actividad. Se recomienda una velocidad de 80 saltos por minuto controlada por la música en un entorno lúdico para los niños. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

Abstract Lipid oxidation is one of the important factors affecting the quality of α-instant rice. In this study, α- instant rice was detected with hydrogen peroxide value (POV), malondialdehyde value (MDA) and fatty acid value (FAV), colorimeter, electronic nose and HS-SPME GC/MS before and after storage. As well as, the effect of catalase treatment on the quality of α-instant rice during storage was investigated The results showed that the content of MDA in α-instant rice decreased by 41.4% in the late stage of the accelerated storage test (21 days), which was 4.3% lower than that of α-instant rice treated with BHT. At the same time, catalase had more advantages in maintaining the whiteness and aroma of α-instant rice, and inhibiting the aldehydes, ketones, hydrocarbons and various volatile odor substances produced by lipid oxidation of α-instant rice. Therefore, catalase, as a natural substitute for synthetic antioxidants, has great potential in the antioxidation of convenience foods.

ABSTRACT BACKGROUND: Computer-aided diagnosis in low-dose (≤ 3 mSv) computed tomography (CT) is a potential screening tool for lung nodules, with quality interpretation and less inter-observer variability among readers. Therefore, we aimed to determine the screening potential of CT using a radiation dose that does not exceed 2 mSv. OBJECTIVE: We aimed to compare the diagnostic parameters of low-dose (< 2 mSv) CT interpretation results using a computer-aided diagnosis system for lung cancer screening with those of a conventional reading system used by radiologists. DESIGN AND SETTING: We conducted a comparative study of chest CT images for lung cancer screening at three private institutions. METHODS: A database of low-dose (< 2 mSv) chest CT images of patients at risk of lung cancer was viewed with the conventional reading system (301 patients and 226 nodules) or computer-aided diagnosis system without any subsequent radiologist review (944 patients and 1,048 nodules). RESULTS: The numbers of detected and solid nodules per patient (both P < 0.0001) were higher using the computer-aided diagnosis system than those using the conventional reading system. The nodule size was reported as the maximum size in any plane in the computer-aided diagnosis system. Higher numbers of patients (102 [11%] versus 20 [7%], P = 0.0345) and nodules (154 [15%] versus 17 [8%], P = 0.0035) were diagnosed with cancer using the computer-aided diagnosis system. CONCLUSIONS: The computer-aided diagnosis system facilitates the diagnosis of cancerous nodules, especially solid nodules, in low-dose (< 2 mSv) CT among patients at risk for lung cancer.

Abstract Doctor moral hazard has a significant effect on the doctor-patient relationship, increases the cost of healthcare, and introduces medical risks. It is a global concern. Doctor moral hazard behaviour is evolving in response to China’s healthcare reform program which was inaugurated in 2009.A scientific understanding of doctor behaviour would facilitate the prevention and control of doctor moral hazard behaviour. This study used the principles and methodology of Glaser and Strauss’s grounded theory. Theoretical and snowball samplings were used to identify 60 subjects. Semi-structured in-depth interviews were conducted with each subject. Themes were identified through substantial (open) coding and theoretical coding. Six types of doctor moral hazard behaviour were extracted from the data. A behavioural model was described and diagrammed to provide a conceptual framework of current doctor moral hazard behaviour. The conceptual model of doctor moral hazard behaviour can be used in several ways to correct or prevent undesirable actions. Rules governing hospital procedures can be strengthened and enforced by supervision and punishment; the asymmetry of information between doctor and patient can be reduced; patient participation in treatment decisions can be increased; the effectiveness of medical ethics education can be improved.

Resumen Para un médico, el riesgo moral tiene un efecto significativo en la relación médico-paciente, incrementa el costo de la atención de salud e introduce riesgos en la salud. Se trata de una preocupación global. El riesgo moral del comportamiento médico ha evolucionado en respuesta al programa de reforma de atención de salud del gobierno de China, inaugurado en 2009. Un entendimiento científico del comportamiento de los médicos facilitaría la prevención y el control del riesgo moral. El presente estudio usa los principios y metodología de la teoría fundamentada de Glaser y Strauss. Se usaron muestras teóricas y multiplicativas para identificar 60 sujetos y realizar entrevistas semiestructuradas en profundidad. Los temas se identificaron mediante codificación sustancial abierta y teórica. De los datos se extrajeron seis tipos de riesgo moral del comportamiento médico. Se describió y diagramó un modelo de comportamiento para proporcionar una estructura conceptual del riesgo moral del comportamiento médico actual. El modelo conceptual de riesgo moral del comportamiento médico puede usarse de varias maneras para corregir o prevenir acciones no deseadas. Las normas procedimentales de los hospitales pueden fortalecerse y exigirse mediante supervisión y castigo; se puede reducir la asimetría de la información que se da entre el médico y el paciente, incrementar la participación del paciente en decisiones de tratamiento y mejorar la efectividad en la educación en ética médica.

Resumo Risco moral médico tem um efeito significativo na relação médico-paciente, aumenta o custo dos cuidados à saúde e introduz riscos médicos. É uma preocupação global. Comportamento de risco moral médico vem se desenvolvendo em resposta ao programa de reforma de cuidados à saúde da China, que se iniciou em 2009. Uma compreensão científica do comportamento médico facilitaria a prevenção e controle do comportamento de risco moral médico. Este estudo utilizou os princípios da metodologia da Teoria Fundamentada de Glaser e Strauss. Amostragem teóricas e por bola de neve foram utilizadas para identificar 60 participantes. Entrevistas detalhadas semi-estruturadas foram realizadas com cada participante. Temas foram identificados através de codificação (aberta) substancial e codificação teórica. Seis tipos de comportamento de risco moral médico foram obtidos dos dados. Um modelo comportamental foi descrito e diagramado de forma a fornecer um enquadre conceitual do comportamento de risco moral médico. O modelo conceitual de comportamento de risco moral médico pode ser utilizado de diversas formas para corrigir ou prevenir ações indesejáveis. Regras que governam procedimentos em hospitais podem ser fortalecidas e reforçadas por supervisão e punição; a assimetria de informações entre médicos e pacientes pode ser reduzida; a participação dos pacientes nas decisões sobre tratamento pode ser aumentada; e a efetividade da educação ética médica pode ser melhorada.

Resumo Introdução: A lesão hepática por isquemia-reperfusão é um processo fisiopatológico comum em cirurgias hepáticas. Mais estudos ainda são necessários para avaliar se o propofol pode reduzir a lesão de isquemia-reperfusão miocárdica induzida pela lesão de isquemia-reperfusão hepática em ratos, juntamente com os mecanismos que estão relacionados. Objetivo: Investigar se propofol protege as células do miocárdio da apoptose com a lesão hepática por isquemia-reperfusão. Métodos: Ratos machos da raça Sprague-Dawley (n = 18) foram alocados aleatoriamente em três grupos: Grupo Sham (Grupo S, n = 6), Grupo Lesão Hepática por Isquemia-reperfusão (Grupo IR, n = 6) e Grupo Propofol (Grupo P, n = 6). O Grupo S foi submetido apenas à laparotomia. O grupo IR foi submetido à isquemia por 30 min e reperfusão por 4 h. O grupo P foi submetido à mesma isquemia do grupo IR, com a administração de 120 mg.kg-1 de propofol iniciada 10min antes da isquemia, seguida de infusão contínua a 20 mg.kg-1.h-1. A apoptose celular foi examinada por meio do ensaio de marcação de terminações dUTP pela deoxinucleotidil transferase. Retículo endoplasmático Ca2+-ATPase2 (SERCA2) e caspase-3 do ácido aspártico contendo cisteína (caspase-3 clivada) foram avaliados com o ensaio western blot e reação em cadeia da polimerase. Resultados: A taxa de apoptose foi maior com mRNA e proteína de SERCA2 regulados para baixo e caspase-3 clivada suprarregulada no Grupo IR, em comparação com o Grupo S (p < 0,01). A taxa de apoptose foi menor com mRNA e proteína de SERCA2 suprarregulada e caspase-3 clivada sub-regulada no Grupo P, em comparação com o Grupo IR (p < 0,01). Conclusões: O propofol pode reduzir a apoptose de células miocárdicas induzida por lesão hepática por isquemia-reperfusão. Entretanto, pode suprarregular o mRNA e a proteína de SERCA2 em ratos.

Abstract Introduction: Hepatic ischemia-reperfusion injury is a common pathophysiological process in liver surgery. Whether Propofol can reduce myocardial ischemia-reperfusion injury induced by hepatic ischemia-reperfusion injury in rats, together with related mechanisms, still needs further studies. Objective: To investigate if propofol would protect the myocardial cells from apoptosis with hepatic ischemia-reperfusion injury. Methods: Male Sprague-Dawley rats (n = 18) were randomly allocated into three groups: Sham Group (Group S, n = 6), Hepatic Ischemia-reperfusion Injury Group (Group IR, n = 6) and Propofol Group (Group P, n = 6). Group S was only subjected to laparotomy. Group IR was attained by ischemia for 30 min and reperfusion for 4 h. Group P was subjected identical insult as in Group IR with the administration of propofol started 10 min before ischemia with 120 mg.kg−1, following by continuous infusion at 20 mg.kg−1.h−1. Cell apoptosis was examined by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay. Endoplasmic reticulum Ca2+-ATPase2 (SERCA2) and cysteine-containing aspartic acid cleaved-caspase3 (cleaved-caspase3) were assayed by western blot and Altimeter polymerase chain reaction. Results: Apoptosis rate was increased, with mRNA and protein of SERCA2 down-regulated and cleaved-caspase3 up-regulated in Group IR compared with Group S (p < 0.01). Apoptosis rate was decreased, with mRNA and protein of SERCA2 up-regulated and cleaved-caspase3 down-regulated in Group P compared with Group IR (p < 0.01). Conclusions: Propofol can reduce hepatic ischemia-reperfusion injury-induced myocardial cell apoptosis, meanwhile, can up-regulate mRNA and protein of SERCA2 in rats.

ABSTRACT Objectives The aim of this study was to explore the effect of capsaicin on expression patterns of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) and trigeminal subnucleus caudalis (Vc) following experimental tooth movement. Material and Methods Male Sprague-Dawley rats were used in this study and divided into small-dose capsaicin+force group, large-dose capsaicin+force group, saline+force group, and no force group. Closed coil springs were used to mimic orthodontic forces in all groups except for the no force group, in which springs were inactivated. Capsaicin and saline were injected into periodontal tissues. Rats were euthanized at 0 h, 12 h, 1 d, 3 d, 5 d, and 7 d following experimental tooth movement. Then, TG and Vc were obtained for immunohistochemical staining and western blotting against CGRP. Results Immunohistochemical results indicated that CGRP positive neurons were located in the TG, and CGRP immunoreactive fibers were distributed in the Vc. Immunohistochemical semiquantitative analysis and western blotting analysis demonstrated that CGRP expression levels both in TG and Vc were elevated at 12 h, 1 d, 3 d, 5 d, and 7 d in the saline + force group. However, both small-dose and large-dose capsaicin could decrease CGRP expression in TG and Vc at 1 d and 3 d following experimental tooth movement, as compared with the saline + force group. Conclusions These results suggest that capsaicin could regulate CGRP expression in TG and Vc following experimental tooth movement in rats.

RESUMO CONTEXTO E OBJETIVO: Enzimas dimetilarginina dimetilaminohidrolase (DDAH), codificadas por genes DDAH1 e DDAH2, desempenham papel fundamental na manutenção da função endotelial. Realizamos estudo tipo caso-controle na população chinesa, com três grupos étnicos (han, kazakh e uygur) para investigar sistematicamente a associação entre a variação de genes DDAH1 e DDAH2 e a hipertensão. DESENHO E LOCAL: Estudo tipo caso-controle no Departamento de Medicina Interna e Diagnóstico Genético, Hospital de Tongji, Tongji Medical College, Universidade de Ciência e Tecnologia de Huazhong. MÉTODOS: Este estudo incluiu 1.224 pacientes com hipertensão e 967 indivíduos saudáveis, sem parentesco, como controles. DDAH1 -396 4 N (GCGT) del > ins, rs3087894, rs805304 and rs9267551 foram genotipados usando o ensaio nuclease TaqMan 5'. RESULTADOS: O genótipo G/C de rs3087894 no DDAH1 foi um fator de risco para a hipertensão arterial no grupo kazakh em modelo codominante (G/C versus G/G; OR 1,39; IC 95%: 1,02-1,88; P < 0,05), com o mesmo resultado no modelo dominante (G/C + C/C versus G/G; OR 1,38; IC 95%: 1,03-1,84; P < 0,05). Em contraste, o genótipo C/C de rs3087894 parecia ser um fator de proteção para a hipertensão no grupo uygur no modelo recessivo (C/C versus G/G + G/C; OR 0,62; IC 95%: 0,39-0,97; P < 0,05). Achado semelhante para rs3087894 também foi observado depois de se ajustar a variante à covariante idade. CONCLUSÃO: Os nossos resultados indicaram que o C-alelo de rs3087894 no DDAH1 foi fator de risco para a hipertensão no grupo de kazakh, mas fator de proteção no grupo de uygur.

CONTEXT AND OBJECTIVE: Dimethylarginine dimethylaminohydrolase enzymes (DDAH), which are encoded by the genes DDAH1 and DDAH2, play a fundamental role in maintaining endothelial function. We conducted a case-control study on a Chinese population that included three ethnic groups (Han, Kazakh and Uygur), to systemically investigate associations between variations in the genes DDAH1 and DDAH2 and hypertension. DESIGN AND SETTING: Experimental study at the Department of Internal Medicine and Genetic Diagnosis, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. METHODS: This case-control study included 1,224 patients with hypertension and 967 healthy unrelated individuals as controls. DDAH1 -396 4N (GCGT) del>ins, rs3087894, rs805304 and rs9267551 were genotyped using the TaqMan 5' nuclease assay. RESULTS: The G/C genotype of rs3087894 in DDAH1 was a risk factor for hypertension in the Kazakh group in the co-dominant model (G/C versus G/G) (OR 1.39; 95% CI: 1.02-1.88; P < 0.05), with the same result in the dominant model (G/C + C/C versus G/G) (OR 1.38; 95% CI: 1.03-1.84; P < 0.05). In contrast, the C/C genotype of rs3087894 seemed to be a protective factor against hypertension in the Uygur group in the recessive model (C/C versus G/G + G/C) (OR 0.62; 95% CI: 0.39- 0.97; P < 0.05). Similar findings for rs3087894 were also observed after adjusting the variable for the age covariate. CONCLUSION: Our results indicated that the C-allele of rs3087894 in DDAH1 was a risk factor for hypertension in the Kazakh group but a protective factor in the Uygur group.

English grain aphid (EGA, Sitobion avenae Fabricius) is an important pest in wheat (Triticum aestivum L.). To develop EGA-resistant varieties, introducing the desirable genes from related species is regarded as an efficient avenue. In this study, the F1, F2 and F3 plants derived from the cross of EGA-susceptible wheat-Psathyrostachys huashanica Keng ex Kuo amphiploid (PHW-SA, AABBDDNsNs) and EGA-resistant triticale (Zhongsi 828, AABBRR) were analyzed for EGA resistance. Consequently, PHW-SA was moderately susceptible while Zhongsi 828 and their F1 hybrids were immune, suggesting that the resistance is dominant. All the F2 plants showed high resistance or immunity over two years, indicating that EGA resistance genes are more likely carried by the rye (Secale cereale L.) chromosomes rather than the genomes A or B of Zhongsi 828. In the F3 generation, 25 of 239 lines became susceptible. Giemsa C-banding patterns revealed that these F3 lines had 38-40 chromosomes, including complete rye genome except 5R (and 2R in five lines). Genomic in situ hybridization analysis confirmed this result. During meiosis, all the chromosomes formed bivalents. Six bivalents in 20 lines and five bivalents in five lines were characterized from rye. In contrast, their F2 parental lines had 42 chromosomes (21 bivalents), containing 1R-7R of rye. No P. huashanica chromosomes were detected. Therefore, we propose that the rye chromosome 5R may be related to EGA resistance.

As lactobacilli possess an antagonistic growth property, these bacteria may be beneficial as bioprotective agents for infection control. However, whether the antagonistic growth effects are attributed to the lactobacilli themselves or their fermentative broth remains unclear. The antagonistic growth effects of Lactobacillus salivarius and Lactobacillus fermentum as well as their fermentative broth were thus tested using both disc agar diffusion test and broth dilution method, and their effects on periodontal pathogens, including Streptococcus mutans, Streptococcus sanguis, and Porphyromonas gingivalisin vitro at different concentrations and for different time periods were also compared. Both Lactobacillus salivarius and Lactobacillus fermentum and their concentrated fermentative broth were shown to inhibit significantly the growth of Streptococcus mutans, Streptococcus sanguis, and Porphyromonas gingivalis, althoughdifferent inhibitory effects were observed for different pathogens. The higher the counts of lactobacilli and the higher the folds of concentrated fermentative broth, the stronger the inhibitory effects are observed. The inhibitory effect is demonstrated to be dose-dependent. Moreover, for the lactobacilli themselves, Lactobacillus fermentum showed stronger inhibitory effects than Lactobacillus salivarius. However, the fermentative broth of Lactobacillus fermentum showed weaker inhibitory effects than that of Lactobacillus salivarius. These data suggested that lactobacilli and their fermentative broth exhibit antagonistic growth activity, and consumption of probiotics or their broth containing lactobacilli may benefit oral health.