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ABSTRACT This study aimed to evaluate the addition of different concentrations of IGF-I and insulin to egg yolk-based extender to improve bovine semen cryopreservation. Two experiments were developed to evaluate the effects of the additives in two commercial extenders, Botubov® (Experiment 1) and Triladyl® (Experiment 2), both with the same design. Three ejaculates from four bulls (n = 12) were used. Each ejaculate was divided into seven equal fractions for dilution (60x106 spermatozoa/mL) in the following treatments: CON: extender only; IGF100: IGF-I 100ng/mL; IGF200: IGF-I 200ng/mL; INS150: insulin 150µUI/mL; INS200: insulin 200µUI/mL; ASS1: IGF-I 100ng/mL + insulin 150µUI/mL; ASS2: IGF-I 200ng/mL + insulin 200µUI/mL. Semen was cryopreserved by an automated system. Post-thawed sperm were evaluated regarding motility by CASA (Computer-assisted sperm analysis), and membranes by fluorescent probes (H342, PI, FITC-PSA and JC-1). For Botubov® extender, INS150 was more efficient in preserving total and progressive motility, VCL, BCF, plasma and mitochondrial membranes. A similar response was seen when insulin was added to the Triladyl® extender, INS150 was more efficient in preserving sperm motility, plasma membrane integrity and mitochondrial potential. Thus, the addition of insulin 150µUI/mL, regardless of the composition of the extender, contributes to better preserving bovine sperm from the cryopreservation effects.

RESUMO Este estudo teve o objetivo de avaliar a adição de diferentes concentrações de IGF-I e insulina a diluidores, à base de gema de ovo, para melhorar a criopreservação do sêmen bovino. Dois experimentos foram desenvolvidos para avaliar os efeitos dos aditivos em dois diluidores comerciais: Botubov® (Experimento 1) e Triladyl® (Experimento 2), ambos com o mesmo delineamento. Foram utilizados três ejaculados de quatro touros (n=12). Cada ejaculado foi dividido em sete frações para diluição (60x106espermatozoides/mL), nos seguintes tratamentos: CON: somente diluidor; IGF100: 100ng/mL de IGF-I; IGF200: 200ng/mL de IGF-I; INS150: 150µUI/mL de insulina; INS200: 200µUI/mL de insulina; ASS1: 100ng/mL de IGF-I + 150µUI/mL de insulina; ASS2: 200ng/mL de IGF-I + 200µUI/mL de insulina. O sêmen foi criopreservado por sistema automatizado. Após a criopreservação, o sêmen foi avaliado quanto à motilidade espermática por CASA e quanto às membranas espermáticas (plasmática, acrossomal e mitocondrial) por sondas fluorescentes (H342, PI, FITC-PSA e JC-1). Para o diluidor Botubov®, INS150 foi mais eficiente em preservar motilidades total e progressiva, VCL, BCF, integridade da membrana plasmática e potencial mitocondrial. Resposta semelhante foi observada quando a insulina foi adicionada ao diluidor Triladyl®, INS150 foi mais eficiente na preservação da motilidade, integridade das membranas e potencial mitocondrial quando comparado aos demais grupos. Assim, a adição de 150µUI/mL de insulina aos diluidores, independentemente da composição, contribui para melhor criopreservação dos espermatozoides bovinos.

https://doi.org/10.1590/1678-4162-12946

An overweight/obesity survey among dogs and cats attended at a veterinary teaching hospital during the second year of the COVID-19 pandemic
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Machado, B.S.

; Bruno, C.E.

; Silva, D.I.

; Barth, J.C.

; Santos, L.P.

; Alves, M.S.

; Araújo, A.C.P.

; Pöppl, Á.G.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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Dez 2022, Volume 74 Nº 6 Páginas 999 - 1006

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Especula-se que a pandemia pelo novo coronavírus SARS-CoV-2 (COVID-19) tenha agravado a prevalência de sobrepeso e obesidade em cães e gatos. O presente artigo teve como objetivo realizar um levantamento de sobrepeso/obesidade, o qual avaliou os registros de 1001 casos consecutivos atendidos no Hospital de Clínicas Veterinárias da Universidade Federal do Rio Grande do Sul, durante a reabertura da instituição ao público, após meses fechada devido à pandemia. Observou-se uma prevalência de sobrepeso ou de obesidade de 36,63%, sendo a frequência ligeiramente maior em gatos (38,37%) do que em cães (35,76%) e não sendo documentado aumento local na prevalência. Castrações (cães e gatos), idade > 10 anos (cães) e sexo masculino (gatos) foram associadas ao sobrepeso/obesidade. Em cerca de 30% das fichas não havia peso registrado, e em cerca de 50% não havia registro de ECC. Essas falhas foram mais frequentes em fichas de felinos e demandam medidas para aumentar o registro desses dados pelas equipes clínicas.

ABSTRACT Anecdotal reports suggest that the new coronavirus SARS-CoV-2 pandemic (COVID-19) has increased overweight and obesity prevalence in dogs and cats. The present report aimed to perform a retrospective overweight/obesity survey on 1,001 consecutive cases attended in the Veterinary Clinics Hospital from the Federal University of Rio Grande do Sul during its reopening to the public after months closed due to the pandemic. The overall overweight or obesity observed prevalence was 36.63%; being the frequency in cats slightly greater (38.37%) than compared to dogs (35.76%). No local increase in these frequencies was detected. Neutering (dogs and cats), age greater than 10 years (dogs), and male gender (cats) were factors associated with overweight/obesity. Bodyweight was not registered in about 30% of the medical records, while the body condition score was not registered in about 50% of the cases. These flaws were more often observed in feline medical records and efforts to improve these data records are warranted.

https://doi.org/10.1590/1678-4162-12696

Clinical presentation and in-hospital prognosis of lung cancer patients presenting with suspected and confirmed COVID-19
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Peixoto, D.

; Callia, J.P.B.

; Bittencourt, M.S.

; Generoso, G.

; Anastácio, V.M.

; Alves-Jr., J.L.

; Silva, T.L. da

; Belizário, J.C.

; Araújo, B.L.M.

; Ho, W.

; Diz, M.D.P.E.

; Hoff, P.M.

; Abdala, E.

; Ibrahim, K.Y.

Brazilian Journal of Medical and Biological Research

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2022, Volume 55 elocation e12140

Resumo: En | Texto: En | PDF: En

We sought to compare the clinical presentation and prognosis of patients with lung cancer and confirmed COVID-19 infection to those with negative RT-PCR SARS-CoV-2 results. We included patients with confirmed lung cancer and suspected COVID-19 who presented to the emergency department. The primary outcome was in-hospital mortality and secondary outcomes included admission to intensive care unit (ICU) or mechanical ventilation. We analyzed the characteristics according to RT-PCR results and primary outcome. We constructed a logistic regression for each RT-PCR result group to find potential predictors of the primary outcome. Among 110 individuals with confirmed lung cancer (65±9 years, 51% male), 38 patients had positive RT-PCR and 72 patients had negative RT-PCR. There was no difference between groups for any clinical characteristic or comorbidities though individuals with confirmed COVID-19 had higher functionality in the ECOG scale. Leucocytes and lymphocytes were lower in individuals with positive tests. The primary outcome occurred in 58 (53%) individuals, 37 (34%) were admitted to the ICU, and 29 (26%) required mechanical ventilation. Although mortality was similar between the two groups, individuals with confirmed COVID-19 were significantly more likely to be admitted to the ICU or receive mechanical ventilation. Only lower lymphocytes and higher CRP were significantly associated with higher mortality. The clinical presentation of COVID-19 in lung cancer is not sufficient to identify higher or lower probability groups among symptomatic individuals, the overall mortality is high irrespective of RT-PCR results, and lymphopenia on admission was associated with the diagnosis and prognosis for COVID-19.

https://doi.org/10.1590/1414-431x2022e12140

Validation of the North America expert consensus statement on reporting CT findings for COVID-19 in individuals with lung cancer
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Peixoto, D.

; Neves, Y.C.S.

; Generoso, G.

; Loureiro, B.M.C.

; Callia, J.P.B.

; Anastácio, V.M.

; Alves Jr., J.L.

; Oshiro, E.M.

; Lima, L.R.

; Sawamura, M.V.Y.

; Auad, R.V.

; Bittencourt, M.S.

; Abdala, E.

; Ibrahim, K.Y.

Brazilian Journal of Medical and Biological Research

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2022, Volume 55 elocation e12376

Resumo: En | Texto: En | PDF: En

The aim of our study was to validate the use of the standardized Radiological Society of North America (RSNA) reporting system in individuals with known lung cancer who presented to the emergency department with suspected COVID-19. We included patients aged 18 years or older from the Cancer Institute of the State of São Paulo (ICESP) with a confirmed diagnosis of lung cancer, admitted to the emergency department and undergoing chest computed tomography (CT) for suspicion of COVID-19. Comparison between SARS-CoV2 RT-PCR across RSNA categories was performed in all patients and further stratified by diagnosis of lung cancer progression. Among 58 individuals included in the analysis (65±9 years, 43% men), 20 had positive RT-PCR. Less than a half (43%) had no new lung findings in the CT. Positive RT-PCR was present in 75% of those with typical findings according to RSNA and in only 9% when these findings were classified as atypical or negative (P<0.001). Diagnostic accuracy was even higher when stratified by the presence or absence of progressive disease (PD). Extent of pulmonary inflammatory changes was strongly associated with higher mortality, reaching a lethality of 83% in patients with >25% of lung involvement and 100% when there was >50% of lung involvement. The lung involvement score was also highly predictive of prognosis in this population as was reported for non-lung cancer individuals. Collectively, our results demonstrated that diagnostic and prognostic values of chest CT findings in COVID-19 are robust to the presence of lung abnormalities related to lung cancer.

https://doi.org/10.1590/1414-431x2022e12376

Morphometry of bovine blastocysts produced in vitro in culture media with antioxidants cysteamine or oily extract of Lippia origanoides
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Sollecito, N.V.

; Alves, R.N.

; Beletti, M.E.

; Pereira, E.C.M.

; Miranda, M.S.

; Silva, J.K.R.

; Borges, A.M.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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Ago 2021, Volume 73 Nº 4 Páginas 799 - 811

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Este estudo teve como objetivo avaliar a morfometria ultraestrutural de embriões bovinos produzidos in vitro e cultivados em diferentes concentrações de antioxidantes. Após a maturação e a fertilização in vitro, os possíveis zigotos foram divididos em cinco tratamentos: T1) sem adição de antioxidantes (controle negativo); T2) adição de 50μM/mL de cisteamina; e T3, T4 e T5) adição de 2,5μg/mL, 5,0μg/mL ou 10,0μg/mL dos antioxidantes derivados do extrato oleoso de Lippia origanoides, respectivamente. No D7 de cultivo, os embriões em estágio de blastocisto foram fixados e preparados para microscopia eletrônica de transmissão. Estes foram avaliados para a proporção entre citoplasma e núcleo, citoplasma e mitocôndria, citoplasma e vacúolos, citoplasma e vacúolos autofágicos e citoplasma e gotículas lipídicas. Blastocistos cultivados em meio contendo extrato oleoso de Lippia origanoides apresentaram características morfológicas como alta relação célula:mitocôndria e baixa relação célula:vacúolos e célula:vacúolo autofágico, possíveis indicadores morfológicos de qualidade embrionária. A massa celular interna (MCI) de blastocistos cultivados em meio sem quaisquer antioxidantes teve a maior razão célula:vacúolo. Resultados semelhantes foram encontrados nas células do trofectoderma (TE) de blastocistos do tratamento 2. Portanto, o meio de cultivo embrionário suplementado com antioxidantes derivados do óleo de Lippia origanoides produziu embriões com maior proporção citoplasmática de organelas, como mitocôndrias. Além disso, tratamentos sem antioxidantes ou com adição de cisteamina apresentaram vacuolização citoplasmática, característica relacionada à produção de embriões de baixa qualidade.

ABSTRACT This study aimed to evaluate the ultrastructural morphometry of bovine embryos produced in vitro grown at different concentrations of antioxidants. After in vitro maturation and fertilization, the presumptive zygotes were assigned into five treatments. T1) without the addition of any antioxidants (negative control); T2) addition of 50μM/mL cysteamine; and T3, T4 and T5) adding 2.5μg/mL, 5.0μg/mL or 10.0μg/mL of the antioxidants derived from the oily extract from Lippia origanoides, respectively. On D7 of culture, the embryos in the blastocyst stage were fixed and prepared for electron transmission microscopy. These were evaluated for the proportion of cytoplasm-to-nucleus, cytoplasm-to-mitochondria, cytoplasm-to-vacuoles, cytoplasm-to-autophagic vacuoles and cytoplasm-to-lipid droplets. Blastocysts cultured in media containing oily extract of Lippia origanoides presented morphological characteristics such as high cell:mitochondria ratio and low cell:vacuoles and cell:autophagic vacuole ratio, possibly been morphological indicators of embryonic quality. Inner cell mass (ICM) from blastocysts cultured in media without any antioxidants had the highest cell:vacuole ratio. Similar results were found in the trophectoderm (TE) cells of blastocysts from treatment 2. Embryo culture media supplemented with antioxidants derived from Lippia origanoides oil produced embryos with a higher cytoplasmic proportion of organelles, such as mitochondria. Also, treatments without any antioxidants or with the addition of cysteamine presented cytoplasmic vacuolization, a characteristic related to production of poor-quality embryos.

https://doi.org/10.1590/1678-4162-12217 150 downloads

Where do we aspire to publish? A position paper on scientific communication in biochemistry and molecular biology
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Baptista, M.S.

; Alves, M.J.M.

; Arantes, G.M.

; Armelin, H.A.

; Augusto, O.

; Baldini, R.L.

; Basseres, D.S.

; Bechara, E.J.H.

; Bruni-Cardoso, A.

; Chaimovich, H.

; Colepicolo Neto, P.

; Colli, W.

; Cuccovia, I.M.

; Da-Silva, A.M.

; Di Mascio, P.

; Farah, S.C.

; Ferreira, C.

; Forti, F.L.

; Giordano, R.J.

; Gomes, S.L.

; Gueiros Filho, F.J.

; Hoch, N.C.

; Hotta, C.T.

; Labriola, L.

; Lameu, C.

; Machini, M.T.

; Malnic, B.

; Marana, S.R.

; Medeiros, M.H.G.

; Meotti, F.C.

; Miyamoto, S.

; Oliveira, C.C.

; Souza-Pinto, N.C.

; Reis, E.M.

; Ronsein, G.E.

; Salinas, R.K.

; Schechtman, D.

; Schreier, S.

; Setubal, J.C.

; Sogayar, M.C.

; Souza, G.M.

; Terra, W.R.

; Truzzi, D.R.

; Ulrich, H.

; Verjovski-Almeida, S.

; Winck, F.V.

; Zingales, B.

; Kowaltowski, A.J.

Brazilian Journal of Medical and Biological Research

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2019, Volume 52 Nº 9 elocation e8935

Resumo: En | Texto: En | PDF: En

The scientific publication landscape is changing quickly, with an enormous increase in options and models. Articles can be published in a complex variety of journals that differ in their presentation format (online-only or in-print), editorial organizations that maintain them (commercial and/or society-based), editorial handling (academic or professional editors), editorial board composition (academic or professional), payment options to cover editorial costs (open access or pay-to-read), indexation, visibility, branding, and other aspects. Additionally, online submissions of non-revised versions of manuscripts prior to seeking publication in a peer-reviewed journal (a practice known as pre-printing) are a growing trend in biological sciences. In this changing landscape, researchers in biochemistry and molecular biology must re-think their priorities in terms of scientific output dissemination. The evaluation processes and institutional funding for scientific publications should also be revised accordingly. This article presents the results of discussions within the Department of Biochemistry, University of São Paulo, on this subject.

https://doi.org/10.1590/1414-431x20198935 1769 downloads

Does the Introgression of Bt Gene Affect Physiological Cotton Response to Water Deficit?
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ROSOLEM, C.A.

; SARTO, M.V.M.

; ROCHA, K.F.

; MARTINS, J.D.L.

; ALVES, M.S.

Planta Daninha

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2019, Volume 37 elocation e019187935

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: O déficit hídrico pode afetar a expressão de proteínas que promovem o controle de lepidópteras no algodoeiro. Entretanto, não é conhecida a resposta de cultivares de algodão Bt e convencionais ao déficit hídrico, o que poderia afetar o potencial de competição com plantas daninhas. O objetivo deste trabalho foi avaliar a resposta de cultivares de algodão Bt e suas isolinhas ao déficit hídrico. Foi conduzido um experimento em casa de vegetação, onde os cultivares de algodão FMT 705, FMT 709 e IMACD 8276, com e sem o gene Bt, se desenvolveram sob dois regimes hídricos: 100% e 50% (déficit hídrico moderado) de água disponível no solo. A fenologia do algodoeiro foi severamente afetada pelo déficit hídrico moderado, com redução da produção de matéria seca de raiz e parte aérea, comprimento e diâmetro de raiz, massa de planta e área foliar. Não foi observado efeito do gene Bt. Déficit hídrico durante o florescimento do algodoeiro resulta em diminuição da condutância estomática, assimilação líquida de CO2 e taxa de transpiração. O potencial hídrico foliar é menor em plantas expostas ao déficit hídrico moderado, em comparação a plantas não estressadas. Contudo, a introgressão do gene Bt não modifica a resposta fisiológica e fenotípica do algodoeiro sob déficit hídrico.

ABSTRACT: Water deficit may affect the expression of lepidoptera-controlling proteins in cotton. However, it is unknown if there is a differential response of conventional and Bt cotton cultivars to water deficit, what could potentially affect the plant competition with weeds. The objective of this work was to investigate the response of Bt cotton cultivars to water deficit compared with their conventional near-isolines. The experiment was conducted in a greenhouse, where the cotton cultivars FMT 705, FMT 709 and IMACD 8276, with and without the Bt gene, were grown under two water regimens: 100% and 50% (moderate water deficit) of available soil water. Cotton phenology was severely affected by moderate water deficit, with a reduction in shoot and root dry matter production, root length and diameter, plant height and leaf area. No effect of the Bt gene was observed. Water deficit during cotton flowering decrease stomatal conductance, net assimilation of CO2 and transpiration rates. The leaf water potential is lower in plants exposed to a moderate water deficit compared with non-stressed plants. However, the introgression of the Bt gene does not modify cotton physiological and phenotypic response to water deficit.

https://doi.org/10.1590/s0100-83582019370100035 985 downloads

Antidiarrheal effect of extract from the bark of Combretum leprosum in mice
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CAVALCANTI, PAULO M.S.

; MARTINS, MARIA DO CARMO C.

; NUNES, PAULO H.M.

; ALVES FILHO, FRANCISCO C.

; SILVA, JANYERSON D.P.

; CAVALCANTI, SUZANA M.G.

Anais da Academia Brasileira de Ciências

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2019, Volume 91 Nº 1 elocation e20170932

Resumo: En | Texto: En | PDF: En

Abstract This study investigated the effects of the ethanolic extract from the bark of Combretum leprosum (ECL) on intestinal transit and castor-oil induced diarrhea in mice. The oral administration of ECL (750 and 1000 mg/kg) slowed intestinal transit (ID50 of 455 mg/kg). The ECL (250-1000 mg/kg) reduced castor-oil induced diarrhea, in a time- and dose-dependent manner (p < 0.05). To determine if antidiarrheal effect of ECL involves α2-adrenergic or opioid receptor activation, the mice were pretreated with antagonists of these receptors, yohimbine or naloxone respectively. None of these drugs inhibited the antidiarrheal effect of ECL. To test if antidiarrheal effect of ECL is due to an antisecretory action, we realized the enteropooling assay on rats. The ECL increased bowel content and did not inhibit intestinal fluid secretion increase induced by misoprostol (100 µg/kg, s.c.). To determine if antimotility effect of ECL is due to a reduction on gastric motility, we realized the organ bath assay in the rat fundus stomach. Isotonic recordings show that the carbachol /KCl - induced contraction was not reversed by the addition of ECL. In conclusion, our results suggest that ECL contains antidiarrheal compounds and these compounds could induce a reduction of intestinal tract motility.

https://doi.org/10.1590/0001-3765201820170932 1558 downloads

Adherence to and invasion of HeLa cells by Campylobacter spp. strains isolated from animals
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Morais-Rios, Paula Alexandra G.

; Alves, Telma M.

; Dorneles, Elaine M.S.

; Stynen, Ana Paula R.

; Cottorello, Ana Cláudia P.

; Lauria-Filgueiras, Ana Luzia

; Lage, Andrey P.

Pesquisa Veterinária Brasileira

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Jul 2018, Volume 38 Nº 7 Páginas 1293 - 1299

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: O objetivo deste estudo foi avaliar a adesão e invasão de células HeLa por amotras de Campylobacter spp. (total n=63) isoladas de frangos (n=4), cães (n=4), primatas não-humanos (n=16), porcos (n=9), fezes de bezerros (n=18), e trato genital de bovinos (n=12). Trinta e duas amostras foram capazes de aderir e 13 invadiram células HeLa. As amostras invasivas incluíram 1 de 4 isolados de cão, 4 de 16 isolados de primatas não-humano (2 C. jejuni e 2 C. coli), 1 de 9 C. coli isoladas de porcos e 7 de 18 C. fetus subsp. fetus isoladas de fezes de bezerros. Apenas 25% dos isolados de frango e de cão e 23% dos isolados de suínos foram capazes de aderir a células HeLa, propriedade exibida por 65% das cepas obtidas a partir de fezes de bezerros e por 83% das amostras isoladas de trato genital bovino. O fenótipo aderente foi observado em 5 de 19, 6 de 15 e 21 de 29 amostras de C. jejuni, C. coli e C. fetus subsp. fetus, respectivamente, enquanto que 3 de 19, 3 de 15 e 7 de 29 amostras foram adicionalmente capazes de invadir as células HeLa, respectivamente. Amostras de C. jejuni, C. coli e C. fetus subsp. fetus isoladas de fezes de animais foram capazes de aderir e invadir as células HeLa, enquanto amostras de C. fetus subsp. fetus isoladas a partir de amostras de trato genital bovino não foram invasivas, em células HeLa. O presente estudo mostrou que amostras de C. jejuni isoladas de primatas não-humanos e cães, C. coli isoladas de primatas não-humanos e porcos, e C. fetus subsp. fetus isolados a partir de fezes de bezerros foram capazes de aderir e invadir células HeLa. Além disso, a falta de capacidade invasiva de amostras de C. fetus subsp. fetus isoladas de trato genital bovino pode ser importante na patogênese das doenças das vias genitais causadas por esta bactéria.

ABSTRACT: The objective of this study was to evaluate the adherence to and invasion of HeLa cells by Campylobacter spp. strains (total n=63) isolated from chickens (n=4), dogs (n=4), non-human primates (n=16), pigs (n=9), calf feces (n=18), and bovine genital tracts (n=12). Thirty-two strains adhered to and 13 invaded HeLa cells. Invasive strains included 1 of 4 dog isolates, 4 of 16 non-human primate isolates (2 C. jejuni and 2 C. coli), 1 of 9 C. coli strains isolated from pigs, and 7 of 18 C. fetus subsp. fetus isolated from calf feces. Only 25% of chicken and dog isolates and 23% of pig isolates were able to adhere to HeLa cells, a property of 65% of strains obtained from calf feces and 83% of bovine genital tract-isolated strains. The adherent phenotype was observed in 5 of 19, 6 of 15, and 21 of 29 strains of C. jejuni, C. coli, and C. fetus subsp. fetus, respectively, whereas 3 of 19, 3 of 15, and 7 of 29 strains were additionally able to invade HeLa cells, respectively. C. jejuni, C. coli, and C. fetus subsp. fetus strains isolated from animal feces are able to adhere and invade HeLa cells, whereas C. fetus subsp. fetus strains isolated from the bovine genital tract were not invasive in HeLa cells. The present study showed that C. jejuni isolated from primates and dogs, C. coli isolated from non-human primates and pigs, and C. fetus subsp. fetus isolated from calf feces have the ability to adhere to and to invade HeLa cells. Moreover, the lack of invasive ability by C. fetus subsp. fetus strains isolated from the bovine genital tract could be important in the pathogenesis of the genital tract diseases caused by this bacterium.

https://doi.org/10.1590/1678-5150-pvb-4999 724 downloads

10.

Effectiveness of Arbuscular Mycorrhizal Fungal Isolates from the Land Uses of Amazon Region in Symbiosis with Cowpea
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SILVA, GLÁUCIA ALVES E

; SIQUEIRA, JOSÉ O.

; STÜRMER, SIDNEY L.

; MOREIRA, FATIMA M.S.

Anais da Academia Brasileira de Ciências

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Mar 2018, Volume 90 Nº 1 Páginas 357 - 371

Resumo: En | Texto: En | PDF: En

ABSTRACT Arbuscular mycorrhizal fungi provide several ecosystem services, including increase in plant growth and nutrition. The occurrence, richness, and structure of arbuscular mycorrhizal fungi communities are influenced by human activities, which may affect the functional benefits of these components of the soil biota. In this study, 13 arbuscular mycorrhizal fungi isolates originating from soils with different land uses in the Alto Solimões-Amazon region were evaluated regarding their effect on growth, nutrition, and cowpea yield in controlled conditions using two soils. Comparisons with reference isolates and a mixture of isolates were also performed. Fungal isolates exhibited a wide variability associated with colonization, sporulation, production of aboveground biomass, nitrogen and phosphorus uptake, and grain yield, indicating high functional diversity within and among fungal species. A generalized effect of isolates in promoting phosphorus uptake, increase in biomass, and cowpea yield was observed in both soils. The isolates of Glomus were the most efficient and are promising isolates for practical inoculation programs. No relationship was found between the origin of fungal isolate (i.e. land use) and their symbiotic performance in cowpea.

https://doi.org/10.1590/0001-3765201820160189 1832 downloads

11.

Interference Between Signal Grass and Cinderella Weed
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MARTINS, P.F.R.B.

; YAMAUTI, M.S.

; ALVES, P.L.C.A.

Planta Daninha

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2018, Volume 36 elocation e018151776

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: O objetivo deste trabalho foi avaliar a coexistência de Urochloa decumbens (capim-braquiária) e Synedrellopsis grisebachii (agriãozinho) num modelo de experimento substitutivo. Para determinação da população crítica, foram plantadas nas proporções de 1, 2, 4, 8, 16, 24 e 32 plantas por vaso e colhidas as partes aéreas, secadas e pesadas, para serem analisadas através de análise de regressão. O experimento substitutivo teve como proporções porcentuais de capim-braquiária e agriãozinho, respectivamente, 100:0, 75:25, 50:50, 25:75 e 0:100. Foram colhidas as partes aéreas, que foram analisadas através dos métodos qualitativos e quantitativos. Foram necessárias 16 plantas para a população crítica do capim-braquiária. A análise qualitativa indica que as duas espécies têm decréscimo na produção de massa em coexistência. A análise quantitativa indica que o agriãozinho possui maior competitividade que o capim-braquiária.

ABSTRACT: The aim in this work was to evaluate the coexistence of Urochloa decumbens (signal grass) and Synedrellopsis grisebachii (Cinderella weed) in a replacement series experiment. To find the critical population density, 1, 2, 4, 8, 16, 24 and 32 plants were planted in the same box, harvested after 40 days, dried and weighted, and analyzed through regression analysis. The replacement series experiment used density percentages for signal grass and Cinderella weed of 100:0, 75:25, 50:50, 25:75 and 0:100. The aboveground parts were harvested, dried and analyzed through qualitative and quantitative measures. Sixteen plants per box were needed for the signal grass to achieve the critical population. Qualitative analysis showed mutual harm to the dry matter allocation of both species. Quantitative analysis showed Cinderella weed to be a better competitor than signal grass.

https://doi.org/10.1590/s0100-83582018360100081 412 downloads

12.

Geospatial distribution and risk factors for bovine cysticercosis in the state of Rondônia, Brazil
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Alves, Wellington C.

; Rossi, Gabriel A.M.

; Lopes, Welber D.Z.

; Almeida, Henrique M.S.

; Mathias, Luis A.

; Vidal, Ana Maria C.

; Soares, Vando E.

Pesquisa Veterinária Brasileira

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Set 2017, Volume 37 Nº 9 Páginas 931 - 936

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: Este estudo objetivou avaliar a prevalência, distribuição espacial e fatores de risco para a ocorrência de cisticercose bovina em animais abatidos oriundos do Estado de Rondônia, Brasil, entre os anos de 2012 a 2015. A prevalência observada foi de 0,014% (I.C. 95% 0,013-0,014), com maior detecção de cisticercos inviáveis (84,80%). Os municípios de Itapuã do Oeste, Candeias do Jamari, Nova Brasilândia D’Oeste, Pimenteiras do Oeste, Porto Velho, Nova Mamoré, Urupá e Guajará-Mirim apresentaram maior risco (OR>1; p<0.05) para a ocorrência da enfermidade quando comparadas ao município de Castanheiras (OR=1). As Regiões Administrativas de Porto Velho, Guajará-Mirim, Colorado D’Oeste, Cacoal, Ji-Paraná, respectivamente, apresentaram maior risco (OR>1; p<0.05) para a ocorrência de cisticercose nos bovinos abatidos comparadas à Região Administrativa de Ariquemes (OR=1). Algumas variáveis como a densidade populacional humana (OR=2,15; 2,15-2,16), percentual de domicílios urbanos com saneamento inadequado (OR=1,91, 1,91-1,92) e percentual de saneamento rural inadequado (OR=1,14, 1,14-1,14) apresentaram-se como fatores de risco significativos (p<0.05) para a ocorrência de cisticercose bovina. Conclui-se que a prevalência de cisticercose bovina no Estado de Rondônia foi 0,014% (I.C. 95% 0,013-0,014) e que áreas de maior ocorrência puderam ser identificadas, fornecendo informações a serem utilizadas pelo Serviço de Inspeção Federal para melhorar a detecção dos casos. Ainda, a densidade da população humana e a deficiência de sistemas de tratamento de esgotos urbanos e rurais estão intimamente relacionadas com a ocorrência da cisticercose bovina neste estado.

ABSTRACT: This study focused on assessing the prevalence, geospatial distribution and risk factors for bovine cysticercosis in cattle from the state of Rondônia, Brazil, through the years 2012 to 2015. The prevalence established was 0.014% (95% C.I. 0.013-0.014), with a higher detection of unviable cysticerci (84.80%). The municipalities of Itapuã do Oeste, Candeias do Jamari, Nova Brasilândia D’Oeste, Pimenteiras do Oeste, Porto Velho, Nova Mamoré, Urupá and Guajará-Mirim had higher risk (OR>1; p<0.05) for cysticercosis occurrence compared with the municipality of Castanheiras (OR=1). The Administrative Regions of Porto Velho, Guajará-Mirim, Colorado D’Oeste, Cacoal, Ji-Paraná had higher risk (OR>1; p<0.05) for cysticercosis occurrence in the slaughtered animals than those reared in Ariquemes Administrative Region (OR=1). Some variables such as human population density (OR=2.15; 2.15-2.16), percentage of urban houses with inappropriate sewage system (OR=1.91, 1.91-.1.92) and percentage of inappropriate rural sewage system (OR=1.14, 1.14-1.14) were significantly associated (p<0.05) with the occurrence of bovine cysticercosis. In conclusion, the prevalence of bovine cysticercosis in the state of Rondônia was 0.014% (95% C.I. 0.013-0.014) and higher-risk areas were identified, providing useful information to Official Sanitary Inspection System in order to improve cysticercosis detection. Also, human population density and the lack of appropriate sewage system in urban and rural areas are closely related to bovine cysticercosis occurrence in this state.

https://doi.org/10.1590/s0100-736x2017000900006 1105 downloads

13.

WEED COMMUNITY INTERFERENCE AND PHYTOSOCIOLOGICAL STUDIES IN A SWEET SORGHUM CROP
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GIANCOTTI, P.R.F.

; MORO, M.S.

; NEPOMUCENO, M.P.

; MARTINS, P.F.R.B.

; BARROSO, A.A.M.

; ALVES, P.L.C.A.

Planta Daninha

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2017, Volume 35 elocation e017154150

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Este estudo teve por objetivo determinar os períodos de interferência e índices fitossociológicos da comunidade infestante da cultura do sorgo-sacarino, híbrido CVSW 80007, cultivado em duas épocas do ano. Os tratamentos consistiram de períodos crescentes de presença ou ausência da comunidade infestante (0, 4, 7, 14, 21, 28, 35, 42, 56 e 68 dias após a emergência da cultura na safra de verão e 0, 4, 7, 14, 21, 28, 35, 42, 49, 56, 63 e 100 dias após a emergência da cultura na safra de outono). A comunidade infestante foi avaliada por meio do número de indivíduos e da massa seca de cada população de planta daninha em cada período, na presença dessas plantas na safra de verão e na presença e ausência na safra de outono. Com esses dados, foram determinados os índices de densidade, dominância e importância relativa das populações, assim como a diversidade e equitatividade da comunidade. As principais plantas daninhas encontradas foram Cyperus rotundus, Alternanthera tenella, Indigofera hirsuta, Amaranthus sp., Digitaria nuda e Portulaca oleracea. A densidade das plantas daninhas foi o fator determinante para a importância delas dentro da comunidade infestante, uma vez que o desenvolvimento e acúmulo de biomassa foram suprimidos pela competição estabelecida pelo sorgo-sacarino. O híbrido de sorgo-sacarino mostrou-se altamente competitivo; nas condições agroecológicas dos experimentos, a interferência exercida pelas populações de plantas daninhas presentes não acarretou redução da produtividade da cultura.

ABSTRACT This research was carried out to evaluate the interference periods and phytosociological indexes of the weed community on sweet sorghum, hybrid CVSW 80007, cultivated for two seasons in a year. The treatments were based on an increased duration of weed presence and weed absence after sowing (0, 4, 7, 14, 21, 28, 35, 42, 56 and 68 days after crop emergence for the summer season and 0, 4, 7, 14, 21, 28, 35, 42, 49, 56, 63 and 100 days for the fall season). The weed community was evaluated based on the number of weeds and the dry mass of each weed population in each period of weed control in the summer season and each period of no weed control and weed control in the autumn season. These data were used to determine the relative indexes of density, dominance and importance, and the weed community indexes of diversity and equitability. The main weeds comprising the community were Cyperus rotundus, Alternanthera tenella, Indigofera hirsuta, Amaranthus sp., Digitaria nuda, and Portulaca oleracea. The weed density was found to be the primary factor for the importance of weeds in the community, once their development and biomass accumulation were suppressed by the sweet sorghum competition. The sweet sorghum hybrid proved highly competitive and, in the agro-ecological conditions of the trials, weed interference did not reduce crop yield.

https://doi.org/10.1590/s0100-83582017350100051 1187 downloads

14.

Update of the Brazilian floristic list of Algae and Cyanobacteria
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Menezes, Mariângela

; Bicudo, Carlos E. M.

; Moura, Carlos W. N.

; Alves, Aigara M.

; Santos, Alana A.

; Pedrini, Alexandre de G.

; Araújo, Andréa

; Tucci, Andrea

; Fajar, Aurelio

; Malone, Camila

; Kano, Cecília H.

; Sant'Anna, Célia L.

; Branco, Ciro Z.

; Odebrecht, Clarisse

; Peres, Cleto K.

; Neuhaus, Emanuel B.

; Eskinazi-Leça, Enide

; Aquino, Eveline

; Nauer, Fabio

; Santos, Gabriel N.

; Amado Filho, Gilberto M.

; Lyra, Goia M.

; Borges, Gyslaine C.P.

; Costa, Iara O.

; Nogueira, Ina de S.

; Oliveira, Ivania B.

; Paula, Joel. C.de

; Nunes, José M. de C.

; Lima, Jucicleide C.

; Santos, Kleber R.S.

; Ferreira, Leandro C.

; Gestinari, Lísia M.S.

; Cardoso, Luciana S.

; Figueiredo, Marcia A.O.

; Silva, Marcos H.

; Barreto, Maria B.B.B.

; Henriques, Maria C.O.

; Cunha, Maria da G.G.S.

; Bandeira-Pedrosa, Maria E.

; Oliveira-Carvalho, Maria F.

; Széchy, Maria T.M.

; Azevedo, Maria T.P.

; Oliveira, Mariana C. de

; Cabezudo, Mariê M.

; Santiago, Marilene F.

; Bergesh, Marli

; Fujii, Mutuê T.

; Bueno, Norma C.

; Necchi Jr., Orlando

; Jesus, Priscila B.

; Bahia, Ricardo G.

; Khader, Samir

; Alves-da-Silva, Sandra M.

; Guimarães, Silvia M.P.B.

; Pereira, Sonia M.B.

; Caires, Taiara A.

; Meurer, Thamis

; Cassano, Valéria

; Werner, Vera R.

; Gama Jr., Watson A. da

; Silva, Weliton J. da

Rodriguésia

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2015, Volume 66 Nº 4 Páginas 1047 - 1062

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Apresenta-se uma síntese atualizada de informações sobre algas no Brasil objetivando refinar os dados reunidos até o presente, bem como avaliar os avanços sobre o conhecimento da diversidade de algas no país desde a publicação do Catálogo de Plantas e Fungos do Brasil. Os resultados de 2015 mostraram um acréscimo de 1.250 espécies (35.7%) a um total de 4.747 em relação a 2010. As classes mais diversas em número de espécies foram Bacillariophyceae, Conjugatophyceae, Florideophyceae, Cyanophyceae, Dinophyceae e Euglenophyceae. Bacillariophyceae e Cyanophyceae tiveram o maior acréscimo de espécies no intervalo de cinco anos. A região Sudeste e Sul foram as mais diversas, porém, as regiões Nordeste com os estados do Piauí e Sergipe e Centro-Oeste com os estados de Mato Grosso, Goiás e Distrito Federal destacaram-se no cenário da biodiversidade nacional. Apesar da escassez de taxonomistas e da infraestrutura limitada, os resultados obtidos evidenciaram um avanço significativo no conhecimento da diversidade de algas no país nesse período de cinco anos, iniciando uma mudança quanto as diferenças geográficas regionais.

Abstract An updated synthesis of cyanobacteria and algae information is presented for Brazil aiming to refine the data gathered to date and evaluate the progress of the biodiversity knowledge about these organisms in the country since the publication of the Catálogo de Plantas e Fungos do Brasil. The results of 2015 showed an increase of 1,250 species (35.7%) when compared to 2010, reaching a total of 4,747 species. The most diverse classes in species number were the Bacillariophyceae, Conjugatophyceae, Florideophyceae, Cyanophyceae, Dinophyceae and Euglenophyceae. Bacillariophyceae and Cyanophyceae had the highest increase in species number in the five-year interval. The Southeast and South regions were the most diverse, however, the Northeast, with the states of Piauí and Sergipe, and the Central-west region, with Mato Grosso, Goiás and Distrito Federal, also stood out in the national algal biodiversity scenario. Despite the shortage of taxonomists and limited infrastructure, the results showed a significant improvement in the knowledge regarding the diversity of cyanobacteria and algae in the country during the study period, starting to even out regional geographical differences caused by subsampling.

https://doi.org/10.1590/2175-7860201566408 4865 downloads

15.

Effects of eugenol on resting tension of rat atria
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Olivoto, R.R.

; Damiani, C.E.N.

; Kassouf Silva, I.

; Lofrano-Alves, M.S.

; Oliveira, M.A.

; Fogaça, R.T.H.

Brazilian Journal of Medical and Biological Research

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Abr 2014, Volume 47 Nº 4 Páginas 328 - 333

Resumo: En | Texto: En | PDF: En

In cardiac and skeletal muscle, eugenol (μM range) blocks excitation-contraction coupling. In skeletal muscle, however, larger doses of eugenol (mM range) induce calcium release from the sarcoplasmic reticulum. The effects of eugenol are therefore dependent on its concentration. In this study, we evaluated the effects of eugenol on the contractility of isolated, quiescent atrial trabeculae from male Wistar rats (250-300 g; n=131) and measured atrial ATP content. Eugenol (1, 3, 5, 7, and 10 mM) increased resting tension in a dose-dependent manner. Ryanodine [100 µM; a specific ryanodine receptor (RyR) blocker] and procaine (30 mM; a nonspecific RyR blocker) did not block the increased resting tension induced by eugenol regardless of whether extracellular calcium was present. The myosin-specific inhibitor 2,3-butanedione monoxime (BDM), however, reversed the increase in resting tension induced by eugenol. In Triton-skinned atrial trabeculae, in which all membranes were solubilized, eugenol did not change resting tension, maximum force produced, or the force vs pCa relationship (pCa=-log [Ca2+]). Given that eugenol reduced ATP concentration, the increase in resting tension observed in this study may have resulted from cooperative activation of cardiac thin filaments by strongly attached cross-bridges (rigor state).

https://doi.org/10.1590/1414-431X20143472 1475 downloads

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