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au:FIGUEIREDO, JOSE ANTONIO POLI DE

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Decision-making for dental pulp exposure: a survey in graduate programs at Brazilian universities
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SCALZILLI, Paulo Augusto

; JARA, Cynthia Mireya

; FLORES, Daniela

; HEINZMANN, Daniela

; FIGUEIREDO, José Antônio Poli de

; SCARPARO, Roberta Kochenborger

Brazilian Oral Research

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2022, Volume 36 elocation e087

Resumo: En | Texto: En | PDF: En

Abstract Clinical decision-making tends to be based on what clinicians have been taught during undergraduate and graduate programs. The aim of the present study was to assess the clinical approach and to identify the factors that influence the decision-making for dental pulp exposure among graduate students and coordinating professors in endodontics programs offered at Brazilian universities. The study used a mail-out survey developed in the Qualtrics platform, based on seven clinical reports in which dental pulp exposure was evidenced. Descriptive statistics showing vital pulp therapy (VPT) and root canal treatment (RCT) were calculated for each clinical report. Data on the participants’ (n = 113) profile and variables related to clinical and radiographic characteristics of the cases were evaluated as to their potential to affect decision-making and analyzed by logistic regression (p < 0.05). VPT was likely to be indicated in cases of patients with immature teeth (OR = 0.017; 95%CI = 0.004 -0.073). RCT indications were related to the presence of symptoms (OR = 5.326; 95%CI = 1.429–19.852) and old age (OR = 21.057; 95%CI=6.809–65.120). In pulp exposure secondary to trauma, time of pulp exposure was significantly associated with RCT indication (OR=3.267; 95%CI=1.332–8.012). The present study demonstrated that patient age, root development, and symptom features were the main factors affecting participants’ decision-making.

https://doi.org/10.1590/1807-3107bor-2022.vol36.0087

Pulp capping with mineral trioxide aggregate or Biodentine: a comparison of mineralized barrier formation and inflammatory and degenerative events
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Reis, Magda de Sousa

; Scarparo, Roberta Kochenborger

; Signor, Bruna

; Bolzan, Juliana Troglio

; Steier, Liviu

; Figueiredo, José Antonio Poli de

Brazilian Oral Research

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2021, Volume 35 elocation e118

Resumo: En | Texto: En | PDF: En

Abstract: Biodentine in endodontic practice has been widely investigated, but comprehensive histological descriptions of degenerative and inflammatory responses are not covered in most of the studies that compare pulp capping materials. This study aimed to evaluate pulpal responses to mineral trioxide aggregate (MTA Angelus) and Biodentine, focusing on mineralized barrier formation and on inflammatory and degenerative events. 80 male Wistar rats were assigned to 5 groups, according to the materials used for pulp capping and coronal sealing (n=8 per group/period). The lower first molars were mechanically exposed, capped with either MTA or Biodentine, and restored with silver amalgam. In an additional test group, the teeth were capped and sealed with Biodentine. The teeth capped with gutta-percha and restored with silver amalgam served as positive control, whereas untreated teeth served as negative control. Pulpal responses and coronal sealing were evaluated after 14 or 21 days. Data was statistically analyzed by the Kruskal-Wallis and Dunn's post hoc tests (p<0.05). Biodentine and MTA presented satisfactory results, showing a milder inflammatory response (p<0.0001) and more pronounced formation of mineralized barrier (p<0.0001) compared to the teeth capped with gutta-percha. As a restorative material, Biodentine kept coronal sealing in only 37.5% of the samples. Biodentine showed favorable properties in vital pulp therapy, being similar to MTA. However, it was not effective in protecting dental pulp from microleakage during the experimental period.

https://doi.org/10.1590/1807-3107bor-2021.vol35.0118 1 downloads

The COVID-19 Pandemic and Planetary Health. A Critical Review of Epidemiology, Prevention, Clinical Characteristics and Treatments for Oral, Head and Neck Health Professionals. Do We Have a Roadmap?
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Jotz, Geraldo Pereira

; Stein, Airton

; Sirena, Sérgio

; Barros, Enrique

; Baldisserotto, Julio

; Figueiredo, José Antônio Poli de

; Lavinsky, Joel

; Steier, Liviu

; Dora, Carlos

International Archives of Otorhinolaryngology

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Set 2020, Volume 24 Nº 3 Páginas 351 - 358

Resumo: En | Texto: En | PDF: En

Abstract Introduction Coronavirus disease 19 (COVID-19) is potentially the greatest global public health crisis of this century. This disease emerged as an outbreak of pneumonia of unknown cause in Wuhan, the capital city of the Hubei province in China, in December 2019. Otolaryngologists, head and neck surgeons and dentists are at an increased risk of occupational disease. Objective The present review summarizes currently published evidence of Covid-19 epidemiology, clinical characteristics, treatment and prevention. No proven effective treatments for this disease currently exist. Data Synthesis COVID-19 started from a zoonotic transmission event associated with a large seafood market that also traded in live wild animals, and it soon became clear that efficient person-to-person transmission was also occurring. Symptoms are varied, and not all patients develop all of them. Conclusion Social distancing seems to have been successful in several places in the world. However, this recommendation alone is not enough to contain the disease, and it is not a long-term solution. Large-scale testing by health professionals of representative samples of the population may give an estimate of the progression of the disease. Different treatments are under test and bring hope of a cure to the population. However, no current treatments (April 27, 2020) have been proven to be the key to success in the treatment of patients with COVID-19. Planetary health is a useful concept to understand the current drivers of this pandemic and to draw a roadmap for science and healthcare that may guide actions to fight economic depression and ensure a healthy recovery.

https://doi.org/10.1055/s-0040-1714143 933 downloads

6ª DIRETRIZES DE MONITORIZAÇÃO AMBULATORIAL DA PRESSÃO ARTERIAL E 4ª DIRETRIZES DE MONITORIZAÇÃO RESIDENCIAL DA PRESSÃO ARTERIAL
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Brandão, Andrea A.

; Alessi, Alexandre

; Feitosa, Audes Magalhães

; Machado, Carlos Alberto

; Figueiredo, Carlos Eduardo Poli de

; Amodeo, Celso

; Rodrigues, Cibele Isaac Saad

; Forjaz, Claudia Lucia de Moraes

; Giorgi, Dante Marcelo Artigas

; Mion Júnior, Décio

; Coelho, Eduardo Barbosa

; Nobre, Fernando

; Lima Júnior, Emilton

; Plavnik, Frida Liane

; Silva, Giovanio Vieira da

; Chaves Júnior, Hilton

; Barbosa, Eduardo Costa Duarte

; Vilela-Martin, José Fernando

; Ribeiro, José Marcio

; Gusmão, Josiane Lima de

; Toledo, Juan Carlos Yugar

; Bortolotto, Luiz Aparecido

; Scala, Luiz Cesar Nazário

; Gomes, Marco Antônio Mota

; Malachias, Marcus Vinícius Bolívar

; Neves, Mario Fritsch Toros

; Wajngarten, Mauricio

; Gus, Miguel

; Passarelli Júnior, Oswaldo

; Jardim, Paulo Cesar Brandão Veiga

; Miranda, Roberto Dischinger

; Paula, Rogério Baumgratz de

; Ferreira Filho, Sebastião Rodrigues

; Andrade, Solange

; Geleilete, Tufik J. M.

; Koch, Vera Hermina K.

; Souza, Weimar Kunz Sebba Barroso de

; Oigman, Wille

Arquivos Brasileiros de Cardiologia

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Mai 2018, Volume 110 Nº 5 Páginas 1 - 29

Resumo: Pt | Texto: Pt | PDF: Pt

https://doi.org/10.5935/abc.20180074 2708 downloads

Dendritic cells and their relation to apical peridontitis
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Figueiredo, José Antonio Poli de

; Machado, Audrey Moraes

; Oliveira, Vinícius Pasqualotti de

; Hartmann, Rafael

; Waltrick, Silvana Beltrami Gonçalves

; Borba, Maristela Gutierrez de

; Brew, Myrian Câmara

; Estrela, Cyntia Rodrigues de Araújo

; Böttcher, Daiana Elisabeth

Brazilian Oral Research

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2018, Volume 32 Supl. 1 elocation e71

Resumo: En | Texto: En | PDF: En

Abstract: The purpose of this manuscript was to re-discuss apical periodontitis, apical biofilm, and its possible relationship with dendritic cells (DC). DCs are potent regulators of the immune system and their function is divided into three categories that involve the presentation of antigens: the presentation of antigens and activation of T cells; a not well established category suggested that DCs induce and maintain immunological tolerance; and the maintenance of the immune memory in conjunction with B cells. DCs in periapical inflammatory lesions are composed of at least two subpopulations that can be distinguished on the basis of ultrastructure and phenotype. These populations might differ in lineage, state of maturation, differentiation, activation, and/or function. The authors hereby analyzed the root apexes of teeth under SEM, after performing apicoectomy due to the failure of conventional endodontic treatment. Microbial biofilm with multispecies and areas of resorption with the presence of Howship lacunae, and images suggestive of denditric cells could be observed. The presence of DCs in periapical lesion could be an indication of the severity of the lesion, with a constant presence of antigen in the periradicular region.

https://doi.org/10.1590/1807-3107bor-2018.vol32.0071 1829 downloads

Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer
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Baldasso, Flávia Emi Razera

; Kopper, Patrícia Maria Poli

; Morgental, Renata Dornelles

; Steier, Liviu

; Figueiredo, José Antônio Poli de

; Scarparo, Roberta Kochenborger

Brazilian Dental Journal

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Dez 2016, Volume 27 Nº 6 Páginas 657 - 663

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Um dos requisistos para a aplicação clínica de materiais endodônticos é apresentar comportamento biológico satisfatório. Neste estudo, as respostas do tecido conjuntivo a cimentos endodônticos à base de silicone (GuttaFlow 2), resina epóxica (AH Plus) e óxido de zinco e eugenol (Endofill) foram comparadas. Doze ratos Wistar tiveram tubos de polietileno (4 em cada animal) contendo um dos materiais e tubos vazios (controle negativo) implantados no tecido conjuntivo subcutâneo. Os tubos foram randomicamente posicionados pelo menos 2 cm distantes entre si e da espinha dorsal do animal. Amostras de tecido contendo os implantes foram processadas para análise histológica após 7 e 60 dias (n=6 animais por período). Células inflamatórias, condensação fibrosa e formação de abscesso foram classificadas em escores de acordo com sua intensidade. O teste Friedman, seguido por post hoc de Dunn, foi empregado para comparar os cimentos. O teste de Mann-Witney U foi empregado para verificar diferenças entre os períodos experimentais em cada grupo (p<0,05). Após 7 dias, a maior parte dos parâmetros histológicos não mostrou diferenças estatísticas entre os grupos. O cimento Endofill promoveu escores mais altos que os outros materiais para a variável células gigantes (p<0,05), além de apresentar um maior número de amostras com formação de abscesso. O GuttaFlow 2 tendeu a apresentar infiltrado inflamatório menos intenso comparado aos outros materiais. Após 60 dias, não houve diferenças estatisticamente significantes entre os grupos na maioria dos parâmetros avaliados. Entretanto, observou-se que o EndoFill apresentou escores mais altos para os macrófagos em comparação ao controle (p<0,05) e que o GuttaFlow 2 apresentou tendência a escores mais baixos que os demais materiais para as variáveis neutrófilos e abscesso. O GuttaFlow 2 apresentou propriedades biológicas apropriadas e pode ser considerado adequado para a prática clínica.

Abstract Satisfactory biological behavior is a necessary requirement for clinical application of endodontic materials. In this study, the connective tissue responses to silicone (GuttaFlow 2), epoxy resin (AH Plus) and zinc oxide and eugenol (Endofill) based sealers were compared. Twelve Wistar rats had polyethylene tubes (four per animal) containing one of the tested sealers and empty tubes (negative control) implanted in their subcutaneous tissue. The tubes were randomly placed 2 cm from the spine and at least 2 cm apart from one another. Tissue samples with implants were processed for histological analysis after 7 or 60 days (n=6 animals per period). Inflammatory cells, fibrous condensation and abscess were scored according to their intensity. Friedman, followed by Dunn's post hoc, was used to compare sealers. Differences between the two experimental periods were verified using Mann-Witney U test (p<0.05). At 7 days, most of the histological parameters showed no significant differences amongst groups. Endofill group scored higher than the others for giant cells (o<0.05) and promoted a greater number of samples presenting abscess formation. GuttaFlow 2 tended to show a less intense inflammatory infiltrate compared to the other materials. At 60 days, there were no significant differences between groups in most of the histological parameters evaluated. However, it was observed that Endofill scored higher for macrophages (p<0.05) compared to the control group, and GuttaFlow 2 tended to present lower scores than the others for neutrophils and abscess. GuttaFlow 2 showed proper biological behavior and should be considered adequate for clinical practice.

https://doi.org/10.1590/0103-6440201600719 2007 downloads

In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers
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WAINSTEIN, Marcela

; MORGENTAL, Renata Dornelles

; WALTRICK, Silvana Beltrami Gonçalves

; OLIVEIRA, Sílvia Dias

; VIER-PELISSER, Fabiana Vieira

; FIGUEIREDO, José Antonio Poli

; STEIER, Liviu

; TAVARES, Cauana Oliva

; SCARPARO, Roberta Kochenborger

Brazilian Oral Research

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2016, Volume 30 Nº 1 elocation e18

Resumo: En | Texto: En | PDF: En

Abstract The aim of this study was to evaluate whether the modification in the silver component is capable of providing GuttaFlow 2 with antibacterial activity against Enterococcus faecalis compared with epoxy resin-based (AH Plus) and zinc oxide and eugenol-based (Endofill) sealers. The antibacterial activity was evaluated using a reference strain of E. faecalis (ATCC 29212). Freshly mixed sealers were subjected to the agar diffusion test (ADT), while the direct contact test (DCT) was performed after materials setting. ADT results were obtained through measurements, in millimeters, of the inhibition zones promoted by the materials, using a digital caliper. In DCT, values of CFU/mL promoted by the three sealers were compared in three experimental periods (1 min, 1 h, and 24 h). The data were analyzed using Kruskal-Wallis and Dunn post-hoc tests (p < 0.05). In both ADT and DCT, GuttaFlow 2 presented no effect against E. faecalis, while Endofill and AH Plus showed similar inhibition zones. Endofill was the only material capable of reducing bacterial growth in DCT. In conclusion, modifications in the silver particle of GuttaFlow 2 did not result in a sealer with antibacterial effect against E. faecalis.

https://doi.org/10.1590/1807-3107BOR-2016.vol30.0018 3710 downloads

LPS levels in root canals after the use of ozone gas and high frequency electrical pulses
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MELO, Tiago André Fontoura de

; GRÜNDLING, Grasiela Sabrina Longhi

; MONTAGNER, Francisco

; SCUR, Alcione Luiz

; STEIER, Liviu

; SCARPARO, Roberta Kochenborger

; FIGUEIREDO, José Antônio Poli de

; VIER-PELISSER, Fabiana Vieira

Brazilian Oral Research

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2016, Volume 30 Nº 1 elocation e19

Resumo: En | Texto: En | PDF: En

Abstract The present study aims to verify the effect of ozone gas (OZY® System) and high frequency electric pulse (Endox® System) systems on human root canals previously contaminated with Escherichia colilipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths standardized to 16 mm. The root canals were prepared up to #60 hand K-files and sterilized using gamma radiation with cobalt 60. The specimens were divided into the following five groups (n = 10) based on the disinfection protocol used: OZY® System, one 120-second-pulse (OZY 1p); OZY® System, four 24-second-pulses (OZY 4p); and Endox® System (ENDOX). Contaminated and non-contaminated canals were exposed only to apyrogenic water and used as positive (C+) and negative (C-) controls, respectively. LPS (O55:B55) was administered in all root canals except those belonging to group C-. After performing disinfection, LPS samples were collected from the canals using apyrogenic paper tips. Limulus Amoebocyte Lysate (LAL) was used to quantify the LPS levels, and the data obtained was analyzed using one-way ANOVA. The disinfection protocols used were unable to reduce the LPS levels significantly (p = 0.019). The use of ozone gas and high frequency electric pulses was not effective in eliminating LPS from the root canals.

https://doi.org/10.1590/1807-3107BOR-2016.vol30.0019 3012 downloads

Bovine Pulp Tissue Dissolution Ability of Irrigants Associated or Not to Ultrasonic Agitation
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Niewierowski, Renata Senger

; Scalzilli, Luíza Rodrigues

; Morgental, Renata Dornelles

; Figueiredo, José Antônio Poli de

; Vier-Pelisser, Fabiana Vieira

; Borba, Maristela Gutierrez de

; Ghisi, Alexandre Corrêa

Brazilian Dental Journal

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Out 2015, Volume 26 Nº 5 Páginas 537 - 540

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo: O objetivo deste estudo foi avaliar a capacidade de dissolução tecidual de diferentes soluções irrigadoras associadas ou não à agitação ultrassônica. Noventa fragmentos de polpa bovina (n=10 por grupo) foram pesados e em seguida colocados individualmente em tubos tipo Eppendorf contendo os seguintes irrigantes: G1- hipoclorito de sódio (NaOCl) a 2,5%; G2- NaOCl a 2,5% + agitação ultrassônica (US); G3- NaOCl a 2,5% + cetramida (CTR) a 0,2%; G4- NaOCl a 2,5% + CTR a 0,2% + US; G5- 400 ppm Sterilox (SX); G6- SX + US; G7- CTR a 0,2%; G8- CTR a 0,2% + US; G9- solução salina. Dois observadores cegados em relação aos grupos experimentais avaliaram as amostras continuamente durante as primeiras 4 h e depois a cada hora pelas próximas 12 h. A velocidade de dissolução foi calculada dividindo o peso inicial da polpa (mg) pelo período de tempo até sua dissolução completa (min). Os dados foram comparados por ANOVA e teste post hoc de Tukey com nível de significância de 5%. Os grupos G1 a G4 dissolveram os fragmentos de polpa completamente e G2 foi significativamente mais rápido do que os outros. Os grupos G5 a G9 não apresentaram atividade de dissolução. Em conclusão, apenas grupos contendo NaOCl foram capazes de dissolver tecido pulpar, o que foi melhorado pela agitação ultrassônica, mas não alterado quando CTR a 0,2% foi associada. Esta solução isolada e o Sterilox não mostraram nenhuma capacidade de dissolução, independentemente da utilização do ultrassom.

Abstract: The aim of this study was to evaluate the tissue dissolution ability of different irrigating solutions associated or not to ultrasonic agitation. Ninety bovine pulp fragments (n=10 per group) were weighed and then placed individually in Eppendorf test tubes containing the following irrigants: G1- 2.5% sodium hypochlorite (NaOCl); G2- 2.5% NaOCl + ultrasonic agitation (US); G3- 2.5% NaOCl + 0.2% cetrimide (CTR); G4- 2.5% NaOCl + 0.2% CTR + US; G5- 400 ppm Sterilox (SX); G6- SX + US; G7- 0.2% CTR; G8- 0.2% CTR + US; G9- saline solution. Two blinded observers assessed the samples continuously for the first 4 h, and then every hour for the next 12 h. Dissolution speed was calculated by dividing the initial pulp weight (mg) by the period of time until complete dissolution (min). Data were compared by ANOVA and Tukey post hoc test with a 5% significance level. G1 to G4 dissolved pulp fragments completely and G2 was significantly faster than the other groups. G5 to G9 did not exhibit dissolving activity. In conclusion, only groups containing NaOCl were capable of pulp tissue dissolution, which was enhanced by ultrasonic agitation, but did not alter when 0.2% cetrimide was associated. This isolated solution and Sterilox showed no dissolving capacity, regardless the use of ultrasound.

https://doi.org/10.1590/0103-6440201300243 1562 downloads

10.

Effect of Different Irrigating Solutions and Photo-Activated Therapy for In Vivo Root Canal Treatment
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López, Fernanda Ullmann

; Kopper, Patrícia Maria Poli

; Bona, Alvaro Della

; Steier, Liviu

; Figueiredo, José Antônio Poli de

; Vier-Pelisser, Fabiana Vieira

Brazilian Dental Journal

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Jun 2015, Volume 26 Nº 3 Páginas 228 - 233

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Este estudo realizou uma avaliação histológica do efeito das soluções irrigadoras Sterilox 400 ppm e hipoclorito de sódio (NaOCl) a 2% e 5%, com e sem a terapia fotodinâmica (PAD), na endodontia em sessão única de dentes de cães associados a lesões periapicais (APs). Dez cães foram divididos de forma randomizada em dois grupos (n=5): com e sem PAD, e os canais radiculares em quatro subgrupos, de acordo com a solução irrigadora utilizada: SX (Sterilox 400 ppm), SH2 (NaOCl 2%), SH5 (NaOCl 5%) e SS (solução salina) como controle positivo. Cento e trinta e quatro canais foram abertos e deixados expostos ao meio oral por 14 dias e depois selados por 60 dias para a indução de APs. Na sequência, os canais foram tratados de acordo com cada protocolo de desinfecção proposto e obturados na mesma sessão. Após 120 dias, os cães foram mortos e os eventos inflamatórios periapicais foram avaliados em microscopia óptica. Os dados qualitativos foram analisados estatisticamente pelos testes Kruskal-Wallis e Mann-Whitney (α=0,05). A PAD, quando empregada após o preparo químico-mecânico, não promoveu diferenças significativas nos escores para inflamação apical (p>0,05). As soluções irrigadoras SX, SH2 e SH5 sem PAD apresentaram diferenças estatísticas do grupo SS (p<0,05) que apresentou os maiores escores para inflamação apical. A PAD não apresentou efeito adicional na endodontia em sessão única dos canais com necrose pulpar associados a lesão apical e a solução irrigadora Sterilox 400 ppm pode ser considerada uma alternativa ao NaOCl na terapia endodôntica.

This study aimed to evaluate histologically the effect of irrigation with 400 ppm Sterilox, 2% and 5% sodium hypochlorite (NaOCl), with and without photo-activated therapy (PAD), in a single-visit root canal treatment of dog's teeth with apical periodontitis (AP). Ten dogs were randomly divided into two groups (n=5): with and without PAD, and the root canals into four subgroups, according to the irrigating solution: SX (400 ppm Sterilox), SH2 (2% NaOCl), SH5 (5% NaOCl) and SS (saline solution) as positive control. A total of 134 root canals were opened and left exposed to the oral environment for 14 days and then sealed for 60 days for AP induction. Then, root canals were treated according to each proposed disinfecting protocol and filled in the same session. After 120 days, the dogs were euthanized and the periapical inflammatory events were evaluated under light microscopy. Qualitative data were submitted to Kruskal-Wallis and Mann-Whitney tests (α=0.05). PAD did not produce significant differences in the scores for apical inflammation when used after chemo-mechanical preparation (p>0.05). The irrigating solutions SX, SH2 and SH5 without PAD were statistically different from SS (p<0.05) that presented the greatest scores for apical inflammation. PAD did not show any additional effect for the treatment of root canals with pulp necrosis and AP in a single visit and 400 ppm Sterilox may be considered an alternative to NaOCl in root canal treatment.

https://doi.org/10.1590/0103-6440201300154 3859 downloads

11.

Are bovine teeth a suitable substitute for human teeth in in vitro studies to assess endotoxin load in root canals?
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MELO, Tiago André Fontoura de

; GRÜNDLING, Grasiela Sabrina Longhi

; MONTAGNER, Francisco

; SCARPARO, Roberta Kochenborger

; FIGUEIREDO, José Antônio Poli de

; VIER-PELISSER, Fabiana Vieira

Brazilian Oral Research

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2015, Volume 29 Nº 1 Páginas 1 - 6

Resumo: En | Texto: En | PDF: En

The present study aimed to determine the feasibility of using bovine teeth as a suitable alternative for human teeth, in experiments involving in vitro endotoxin contamination. Twenty bovine central incisors and 20 human single-root premolars had their dental crowns removed and root lengths set at 16 mm. Root canals were prepared up to #60 K-file size and sterilized with cobalt-60 gamma irradiation (20 kGy, 6 h). The teeth were randomly divided into four groups: G1-bovine teeth (bovine negative control, n = 10), G2-human teeth (human negative control, n = 10), G3-bovine teeth, inoculated withEscherichia coli (055:B55) LPS, and G4-human teeth inoculated with E. coli LPS. The G1 and G2 groups were exposed to apyrogenic water. After the teeth had been incubated at 37 °C and atmospheric humidity for 24 h, the samples of solutions in the main canals were collected with apyrogenic absorbent paper tips. LPS levels were quantified using Limulus Amebocyte Lysate assay. The data obtained were statistically analyzed using one-way ANOVA, with a significance level of 5%. A high amount of endotoxin was detected in the inoculated human teeth (G4) when compared to the sterilized teeth (G2), as well as in the inoculated bovine teeth (G3) when compared to the inoculated human teeth (G4). However, there was no statistical difference between bovine teeth before and after the E. coli endotoxin inoculation. Therefore, under the mentioned experimental conditions, the use of bovine teeth should not be a choice for laboratory research on endotoxin contamination.

https://doi.org/10.1590/1807-3107BOR-2015.vol29.0087 2339 downloads

12.

Detection of Apical Inflammatory Root Resorption Associated with Periapical Lesion Using Different Methods
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Estrela, Carlos

; Guedes, Orlando Aguirre

; Rabelo, Luiz Eduardo G.

; Decurcio, Daniel Almeida

; Alencar, Ana Helena G

; Estrela, Cyntia R.A.

; Figueiredo, José Antônio Poli de

Brazilian Dental Journal

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Out 2014, Volume 25 Nº 5 Páginas 404 - 408

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

O objetivo deste estudo foi detectar reabsorção radicular inflamatória apical (RRIA) associada à lesão periapical utilizando tomografia computadorizada de feixe cônico (TCFC) e microscopia eletrônica de varredura (MEV). Este estudo clínico avaliou RRIA em 88 ápices radiculares de 52 dentes permanentes de 14 pacientes, extraídos por diferentes motivos. Os pacientes foram submetidos a uma entrevista clínica, revisão da história médica/dental, exames clínicos e de imagem para o plano de tratamento. Todos os dentes selecionados apresentaram condição não restaurável devido à extensa perda de estrutura dental associada a lesões cariosas, e infecção do canal radicular associada a lesões periapicais. TCFC foram obtidas dos pacientes com o objetivo de diagnosticar as alterações periapicais que se mostraram complexas ou duvidosas. Dois examinadores avaliaram a presença ou ausência de RRIA. Os ápices foram também analisados por MEV. O teste do qui-quadrado foi usado para comparar os métodos de detecção de RRIA. O nível de significância foi estabelecido em 5%. RRIA associada à infecção do canal radicular e periodontite apical foi encontrada em 61,4% dos casos estudados usando MEV, e pelo menos metade dos casos utilizando TCFC. A análise microscópica continua a ser o padrão frente a métodos de imagens para a identificação de RRIA.

The aim of this study was to detect apical inflammatory root resorption (AIRR) associated with periapical lesion using cone beam computed tomography (CBCT) and scanning electronic microscopy (SEM). This clinical study evaluated AIRR in 88 root apexes, from 52 permanent teeth of 14 patients, extracted for different reasons. The patients were submitted to a clinical interview, review of dental/medical histories and clinical/imaging examinations for treatment planning. All selected teeth showed unrestorable condition because of the extensive coronal breakdown due to carious lesions, and root canal infection associated with periapical lesions. CBCT images were obtained from the patients with the aim of diagnosing the periapical diseases which showed complex or doubtful conditions. Two examiners assessed the presence or absence of AIRR. Apices were also analyzed under SEM. Chi-square test was used to compare the imaging methods for detection of AIRR. The level of statistical significance was set at 5%. AIRR associated with root canal infection and apical periodontitis was found in 61.4% of the cases studied by using SEM, and at least half of the cases by CBCT. The microscopic analysis remains as a reference standard against the imaging method to identify AIRR.

https://doi.org/10.1590/0103-6440201302432 4452 downloads

13.

Effect of Super-Oxidized Water, Sodium Hypochlorite and EDTA on Dentin Microhardness
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Ghisi, Alexandre Corrêa

; Kopper, Patrícia Maria Poli

; Baldasso, Flávia E. R.

; Stürmer, Caroline P.

; Rossi-Fedele, Giampiero

; Steier, Liviu

; Figueiredo, José Antonio Poli de

; Morgental, Renata Dornelles

; Vier-Pelisser, Fabiana Vieira

Brazilian Dental Journal

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Out 2014, Volume 25 Nº 5 Páginas 420 - 424

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

O presente estudo teve como objetivo avaliar a influência das seguintes soluções irrigadoras na microdureza da dentina do canal radicular: hipoclorito de sódio a 2% (NaOCl2), hipoclorito de sódio a 5% (NaOCl5), água superoxidada (Sterilox(r) 400 ppm - Sx) e EDTA a 17% (E). Oitenta raízes de incisivos bovinos foram divididas aleatoriamente em 8 grupos (n=10): NaOCl2, NaOCl5, Sx e NaOCl2 + E, NaOCl5 + E, Sx + E (associados ao E como irrigante final por 5 min), E isolado e água destilada (H2Od), como controle negativo. O preparo dos canais radiculares foi realizado com instrumentos manuais, usando um dos protocolos de irrigação por 30 min. A seguir, 5 mm do terço cervical de cada amostra foram cortados perpendicularmente e submetidos ao teste de microdureza de Vickers, em dois pontos, um aproximadamente 500-1000 µm da luz do canal radicular (distância 1), e o outro aproximadamente 500-1000 µm da superfície externa da raiz (distância 2). Os dados foram analisados pelos testes de Wilcoxon e Kruskal-Wallis com um nível de significância de 5%. Os valores de microdureza na distância 1 foram significativamente menores do que na distância 2 para todos os grupos, exceto NaOCl5 e NaOCl5 +E (p>0,05). O EDTA mostrou os menores valores de microdureza. No entanto, não foi detectada diferença estatisticamente significativa entre os grupos na distância 1 e o EDTA foi significativamente diferente apenas do Sx na distância 2. Pode-se concluir que todas as soluções testadas mostraram menor microdureza na camada de dentina mais superficial do canal radicular em comparação aos valores encontrados próximo à superfície radicular externa, exceto NaOCl5 e NaOCl5 + E; o EDTA promoveu menor microdureza em comparação ao Sterilox(r) neste ponto.

The present study aimed to evaluate the influence of the following irrigating solutions on the microhardness of root canal dentin: 2% sodium hypochlorite (2NaOCl), 5% sodium hypochlorite (5NaOCl), super-oxidized water (400 ppm Sterilox - Sx) and 17% EDTA (E). Eighty roots from bovine incisors were randomly divided into 8 groups (n=10): 2NaOCl, 5NaOCl, Sx, and 2NaOCl + E, 5NaOCl + E, Sx + E (associated with E as final irrigant for 5 min), E solely and distilled water (dH2O) as the negative control. Root canal preparation was performed by hand instruments, using one of the irrigation protocols for 30 min. Then, 5 mm of the cervical root third were cut out from each sample and subjected to the Vickers microhardness test, at two points, one at approximately 500-1000 µm from the root canal lumen (distance 1), and the other at approximately 500-1000 µm from the external root surface (distance 2). Data were analyzed by Wilcoxon and Kruskal-Wallis tests at 5% significance level. Microhardness values at distance 1 were significantly lower than those at distance 2 for all groups, except 5NaOCl and 5NaOCl + E groups (p>0.05). EDTA showed the lowest microhardness values. However, no statistically significant difference was detected among groups at distance 1 and EDTA was significantly different only from Sx at distance 2. In conclusion, all tested solutions showed lower microhardness at the most superficial root canal dentin layer compared to the one found near the external root surface, except 5NaOCl and 5NaOCl + E; EDTA promoted lower microhardness values in comparison to Sterilox at this site.

https://doi.org/10.1590/0103-6440201300129 3558 downloads

14.

Influence of apical foramen lateral opening and file size on cemental canal instrumentation
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Souza, Ronaldo Araújo

; Sousa, Yara T. Corrêa Silva

; Figueiredo, José Antônio Poli de

; Dantas, João da Costa Pinto

; Colombo, Suely

; Pécora, Jesus Djalma

Brazilian Dental Journal

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Abr 2012, Volume 23 Nº 2 Páginas 122 - 126

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Tendo em vista que a instrumentação do forame apical tem sido sugerida para a limpeza e desinfecção do canal cementário, a seleção do calibre do instrumento e a posição do forame apical representam passos desafiantes. Este estudo analisou a influência que a saída lateral do forame apical e o calibre do instrumento podem exercer na instrumentação do canal cementário. Trinta e quatro incisivos centrais superiores foram divididos em dois grupos: Grupo 1 (n=17), sem preparo cervical, e Grupo 2 (n=17), com preparo cervical com brocas LA Axxess. Limas K com aumentos de diâmetro foram progressivamente inseridas no canal até ajustar no forame apical e as pontas ficarem visíveis e foram fixadas com adesivo de etil cianoacrilato. Os conjuntos raízes/limas foram seccionados transversalmente a 5 mm aquém do ápice. Os ápices foram examinados por microscopia eletrônica de varredura com aumento de 140x e imagens digitais foram capturadas. Os dados foram examinados estatisticamente pelo teste t de Student e teste exato de Fischer com nível de significância de 5%. A microscopia eletrônica de varredura mostrou que 19 (56%) dos forames apicais saíram lateralmente em relação ao ápice radicular, enquanto que 15 (44%) coincidiram com ele. Dificuldade significantemente maior para chegar ao forame apical foi observada no Grupo 2. Os resultados sugerem que quanto mais calibroso for o instrumento foraminal, mais difícil poderá ser a instrumentação do forame apical em canais cementários que apresentam saída lateral.

Since instrumentation of the apical foramen has been suggested for cleaning and disinfection of the cemental canal, selection of the file size and position of the apical foramen have challenging steps. This study analyzed the influence of apical foramen lateral opening and file size can exert on cemental canal instrumentation. Thirty-four human maxillary central incisors were divided in two groups: Group 1 (n=17), without flaring, and Group 2 (n=17), with flaring with LA Axxess burs. K-files of increasing diameters were progressively inserted into the canal until binding at the apical foramen was achieved and tips were visible and bonded with ethyl cyanoacrylate adhesive. Roots/files set were cross-sectioned 5 mm from the apex. Apices were examined by scanning electron microscopy at ×140 and digital images were captured. Data were analyzed statistically by Student’s t test and Fisher’s exact test at 5% significance level. SEM micrographs showed that 19 (56%) apical foramina emerged laterally to the root apex, whereas 15 (44%) coincided with it. Significantly more difficulty to reach the apical foramen was noted in Group 2. Results suggest that the larger the foraminal file size, the more difficult the apical foramen instrumentation may be in laterally emerged cemental canals.

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15.

Interference of partial visual analysis of root filling quality and apical status on retreatment decisions
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Morgental, Renata Dornelles

; Santos, Régis Burmeister dos

; Rösing, Cassiano Kuchenbecker

; Chanin, Taís do Amaral

; Figueiredo, José Antônio Poli de

Journal of Applied Oral Science

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Abr 2012, Volume 20 Nº 2 Páginas 206 - 211

Resumo: En | Texto: En | PDF: En

OBJECTIVE: The presence of periapical radiolucency has been used as a criterion for endodontic treatment failure. However, in addition to the inherent limitations of radiographic examinations, radiographic interpretations are extremely subjective. Thus, this study investigated the effect of partial analysis of root filling quality and periapical status on retreatment decisions by general dentists. MATERIAL AND METHODS: Twelve digitalized periapical radiographs were analyzed by 10 observers. The study was conducted at three time points at 1-week intervals. Radiographs edited with the Adobe Photoshop CS4 software were analyzed at three time points: first, only root filling quality was analyzed; second, only the periapical areas of the teeth under study were visualized; finally, observers analyzed the unedited radiographic image. Spearman ’s coefficient was used to analyze the correlations between the scores assigned when the periapical area was not visible and when the unedited radiograph was analyzed, as well as between the scores assigned when root fillings where not visible and when the unedited radiograph was analyzed. Sensitivity, specificity, positive and negative predictive values between partial images and unedited radiographs were also used to analyze retreatment decisions. The level of significance was set at 5%. RESULTS: The visualization of the root filling on the unedited radiograph affected the interpretation of the periapical status and the technical quality of the fillings has a greater influence on the general dentist’s decision to prescribe endodontic retreatment than the periapical condition. CONCLUSION: In order to make endodontic diagnosis, radiographic interpretation process should not only emphasize technical aspects, but also consider biological factors.

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