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ABSTRACT The Northeast region of Brazil is the primary producer of yellow passion fruit. In recent years, water scarcity has led passion fruit growers to use highly saline water for cultivation. Therefore, implementing technologies that alleviate the negative effects of salt stress on plants is a promising approach, particularly in semi-arid conditions. This study aimed to assess the efficacy of organomineral fertilizers in mitigating salt stress effects on chlorophyll-a fluorescence and gas exchange in ‘Guinezinho’ yellow passion fruit seedlings. The treatments were arranged in randomized blocks with four replicates and four seedlings per plot, following a 2 × 3 factorial design. The factors considered were irrigation using water with low (0.18 dS m-1) and high (4.0 dS m-1) electrical conductivity and three liquid organomineral attenuators (Codasal™, Aminoagro Raiz™, and a mixture of Codasal™ + Aminoagro Raiz™ at a 1:1 v/v ratio), applied through fertigation. Irrigation with water containing 4.0 dS m-1 electrical conductivity, combined with liquid fertilizers, resulted in increased substrate salinity, and reduced stomatal conductance in yellow passion fruit seedlings, particularly when Codasal™ was applied. Application of Codasal™ alone or in combination with Aminoagro Raiz™ significantly enhanced variable fluorescence, quantum efficiency of PSII, CO2 assimilation rate, and instantaneous carboxylation efficiency in ‘Guinezinho’ yellow passion fruit seedlings.

RESUMO O Nordeste é a principal região produtora de frutos de maracujazeiro-amarelo no Brasil, que devido à escassez hídrica ocorrida nos últimos anos, os passifloricultores utilizam água de elevada concentração de sais para exploração da cultura. Dessa forma, o emprego de tecnologias que possibilite a atenuação do estresse salino às plantas é uma estratégia promissora nas condições de semiárido. Objetivou-se com o trabalho avaliar a ação de fertilizantes organominerais como atenuador do estresse salino sobre a fluorescência da clorofila a e trocas gasosas de mudas maracujazeiro-amarelo acesso ‘Guinezinho’. Os tratamentos foram distribuídos em blocos casualizados com quatro repetições e quatro mudas por parcela, empregando o esquema fatorial 2 × 3, referente às irrigação com água de baixa (0,18 dS m-1) e alta (4,00 dS m-1) condutividade elétrica e três atenuadores organomineral líquidos (Codasal®, Aminoagro Raiz® e mistura de Codasal® + Aminoagro Raiz® - proporção de 1/1 v/v), via fertirrigação. A irrigação com água de 4,0 dS m-1 associado com fertilizantes líquidos aumentam a salinidade do substrato e reduz a condutância estomática das mudas de maracujazeiro-amarelo, principalmente, com aplicação de Codasal®. A aplicação individual ou associado de Codasal® com Aminoagro Raiz® promove aumentos na fluorescência variável, eficiência quântica do PSII, taxa de assimilação de CO2 e eficiência instantânea de carboxilação em mudas de maracujazeiro-amarelo, acesso “Guinezinho”.

https://doi.org/10.1590/1807-1929/agriambi.v27n11p839-847

Leaching of salts and production of sour passion fruit irrigated with low- and high-salinity water
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Nunes, Járisson C.

; Lima Neto, Antonio J. de

; Cavalcante, Lourival F.

; Pereira, Walter E.

; Gheyi, Hans R.

; Lima, Geovani S. de

; Oliveira, Flaviano F. de

; Nunes, Juliete A. da S.

Revista Brasileira de Engenharia Agrícola e Ambiental

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May 2023, Volumen 27 Nº 5 Paginas 393 - 399

Resumen: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT The objective of this study was to evaluate the effect of irrigation with low- and high-salinity water on the increment of salts in the soil, the production components of sour passion fruit and the leaching of salts by rainfall and by leaching fraction of 10%. The treatments were arranged in randomized blocks, in a split-plot scheme, corresponding to three irrigation management practices [evaluation of soil chemical attributes before irrigation; and after irrigation with water of low electrical conductivity (0.35 dS m-1), and high electrical conductivity (4.00 dS m-1) in the main plot], and in the subplot two soil depths of evaluation (0-20 and 20-40 cm) and three soil sampling times (at 115 days after transplanting of seedlings (DAT) - beginning of flowering; at 199 DAT - end of the dry season; and at 379 DAT - end of the rainy season). Irrigation increased the electrical conductivity of the soil saturation extract, with higher values in the surface layer and at the end of the dry season. Rainfall during the rainy season reduced the saline character of soil from moderately saline and strongly saline, in treatments irrigated with water of low and high electrical conductivity, to non-saline and slightly saline, respectively. Irrigation with high-electrical conductivity water negatively affected the production components and yield of sour passion fruit.

RESUMO O objetivo deste estudo foi avaliar o efeito da irrigação com água de baixa e alta salinidade sobre o incremento de sais no solo, os componentes de produção do maracujazeiro azedo e a lixiviação dos sais pela precipitação pluviométrica e pela fração de lixiviação de 10%. Os tratamentos foram dispostos em blocos ao acaso, em esquema de parcelas subdivididas, correspondendo a três práticas de manejo de irrigação [avaliação dos atributos químicos do solo antes da irrigação; e após irrigação com água de baixa (0,35 dS m-1) e alta condutividade elétrica (4,00 dS m-1) na parcela principal], e na subparcela duas profundidades de avaliação dos atributos químicos do solo (0-20 e 20-40 cm) e três épocas de amostragem de solo (aos 115 dias após o transplantio das mudas (DAT) - início do florescimento das plantas; aos 199 DAT - final do período de estiagem; e aos 379 DAT - final do período chuvoso). A irrigação elevou a condutividade elétrica do extrato de saturação do solo, com superioridade na camada superficial e no final do período seco. A precipitação pluviométrica durante a estação chuvosa reduziu o caráter salino do solo de medianamente e fortemente salino, nos tratamentos irrigados com água de baixa e alta condutividade elétrica, para não salino e ligeiramente salino, respectivamente. A irrigação com água de alta condutividade elétrica afetou negativamente os componentes de produção e produtividade do maracujazeiro azedo.

https://doi.org/10.1590/1807-1929/agriambi.v27n5p393-399

Healthcare workers exposed to COVID-19 patients present an inflammatory status and Th2/Th17/Th22 immune profile: findings from before vaccine application in Brazil
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AQUINO, RODRIGO CESAR A. DE

; BARROS, BÁRBARA RAFAELA S.

; SILVA, GUILHERME ANTONIO S.

; SOUSA, GEORON F. DE

; SOUZA, EDSON B. DE

; SILVA, DYEGO R.C.

; NASCIMENTO, ARIONE V. DO

; SÁ, IGOR W.A. DE

; LIMA, ELKER L.S. DE

; SILVA, BÁRBARA O.

; LIMA, LUÍSA P.O. DE

; VIEIRA, AMANDA M.

; BARBOSA NETO, ADAUTO G.

; MARCOS, BIANCA F. SÃO

; SILVA, ANNA JÉSSICA D.

; OLIVEIRA, TALITA HELENA ARAÚJO DE

; CARVALHO, BRUNO M.

; MUNIZ, MARIA TEREZA C.

; FREITAS, ANTONIO CARLOS DE

; CAMPELO JÚNIOR, EVÔNIO B.

; MELO, CRISTIANE M.L. DE

Anais da Academia Brasileira de Ciências

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2023, Volumen 95 Supl. 1 elocation e20220502

Resumen: En | Texto: En | PDF: En

Abstract Healthcare workers present an increased risk of contagion for the SARS-CoV-2 virus due to their labor exposure. Here, we describe the clinical, laboratory, and immunological characteristics of healthcare workers, before vaccine application, exposed to SARS-CoV-2-infected patients. We collected sociodemographic, clinical, and laboratory information from 50 professionals who worked during the COVID-19 pandemic at the Clinical Hospital of the Northwest in Brazil. The results showed that most workers are women, over 50 years old, and worked as nursing technicians. Approximately 56% of workers were positive for a previous infection by RT-PCR and/or anti-SARS-CoV-2-immunoglobulin tests. Increased levels of hematocrit, neutrophils, NK lymphocytes, and fibrinogen, were found in positive healthcare workers, suggesting a light inflammatory status. The immunological findings showed an increase in IL-17 production and a Th2/Th17/Th22 profile followed by high serology for anti-SARS-CoV-2 IgM and IgG. Those data reveal the importance of studies with healthcare workers to investigate if the continuous exposition to the virus may result in chronic activation of the immune system and/or pulmonary inflammation in this target group.

https://doi.org/10.1590/0001-3765202320220502

Samanea tubulosa Benth. (Fabaceae): Antinociceptive effect on acute pain in mice: K+ATP channel and opioid activity
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ALIXANDRE, TAMNATA F.

; SOUSA, RENATO P.

; GOMES, BRUNO S.

; SILVA, ARÊTHA H.S.

; SOUSA NETO, BENEDITO P.

; SOUSA, ELCILENE A.

; LIMA, MARLUCE P.D.

; LOPES, EVERTON M.

; PIAUILINO, CELYANE A.

; NASCIMENTO, REJANE T.

; REIS FILHO, ANTÔNIO C.

; ALMEIDA, FERNANDA R.C.

; OLIVEIRA, FRANCISCO A.

; CHAVES, MARIANA H.

; COSTA, LUCIANA M.

; ALVES, MICHEL M. MORAES

; COSTA, AMILTON P.R.

Anais da Academia Brasileira de Ciências

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2022, Volumen 94 Nº 2 elocation e20210715

Resumen: En | Texto: En | PDF: En

Abstract Samanea tubulosa Benth. it has been widely used in traditional medicine to treat inflammatory processes. The present study aimed to investigate the antinociceptive effect and mechanism of action of the fractions obtained from the Samanea tubulosa pods in mice. The antinociceptive activity was evaluated in formalin, capsaicin and glutamate tests and the. The possible mechanisms of action involved in the antinociceptive effect of the hexane and ethyl acetate fraction in the opioid system, also the the K + ATP channels and the L-arigine pathways of nitric oxide were evaluated. The chemical characterization analysis revealed in the hexane fraction the presence of triterpenes such as lupenone and lupeol. In the glutamate test, the hexane and ethyl acetate fractions showed antinociceptive activity at the dose of 12.5 and 25 mg kg-1. The antinociception produced by the hexane and ethyl acetate fractions was significantly reversed by naloxone, indicating that the fractions act through the opioid pathway. Antinociceptive response of the ethyl acetate fraction was blocked by glibenclamide, indicating that this fraction acts via the K + ATP channels activation. It is concluded that the fractions under study exert antinociceptive activity possibly related to the opioid route and through K+ ATP channels activation.

https://doi.org/10.1590/0001-3765202220210175

Persistent symptoms and decreased health-related quality of life after symptomatic pediatric COVID-19: A prospective study in a Latin American tertiary hospital
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Fink, Thais T.

; Marques, Heloisa H.S.

; Gualano, Bruno

; Lindoso, Livia

; Bain, Vera

; Astley, Camilla

; Martins, Fernanda

; Matheus, Denise

; Matsuo, Olivia M.

; Suguita, Priscila

; Trindade, Vitor

; Paula, Camila S.Y.

; Farhat, Sylvia C.L.

; Palmeira, Patricia

; Leal, Gabriela N.

; Suzuki, Lisa

; Odone Filho, Vicente

; Carneiro-Sampaio, Magda

; Duarte, Alberto José S.

; Antonangelo, Leila

; Batisttella, Linamara R.

; Polanczyk, Guilherme V.

; Pereira, Rosa Maria R.

; Carvalho, Carlos Roberto R.

; Buchpiguel, Carlos A.

; Latronico, Ana Claudia

; Seelaender, Marilia

; Silva, Clovis Artur

; Pereira, Maria Fernanda B.

; Sallum, Adriana M. E.

; Brentani, Alexandra V. M.

; Neto, Álvaro José S.

; Ihara, Amanda

; Santos, Andrea R.

; Canton, Ana Pinheiro M.

; Watanabe, Andreia

; Santos, Angélica C. dos

; Pastorino, Antonio C.

; Franco, Bernadette D. G. M.

; Caruzo, Bruna

; Ceneviva, Carina

; Martins, Carolina C. M. F.

; Prado, Danilo

; Abellan, Deipara M.

; Benatti, Fabiana B.

; Smaria, Fabiana

; Gonçalves, Fernanda T.

; Penteado, Fernando D.

; Castro, Gabriela S. F. de

; Gonçalves, Guilherme S.

; Roschel, Hamilton

; Disi, Ilana R.

; Marques, Isabela G.

; Castro, Inar A.

; Buscatti, Izabel M.

; Faiad, Jaline Z.

; Fiamoncini, Jarlei

; Rodrigues, Joaquim C.

; Carneiro, Jorge D. A.

; Paz, Jose A.

; Ferreira, Juliana C.

; Ferreira, Juliana C. O.

; Silva, Katia R.

; Bastos, Karina L. M.

; Kozu, Katia

; Cristofani, Lilian M.

; Souza, Lucas V. B.

; Campos, Lucia M. A.

; Silva Filho, Luiz Vicente R. F.

; Sapienza, Marcelo T.

; Lima, Marcos S.

; Garanito, Marlene P.

; Santos, Márcia F. A.

; Dorna, Mayra B.

; Aikawa, Nadia E.

; Litvinov, Nadia

; Sakita, Neusa K.

; Gaiolla, Paula V. V.

; Pasqualucci, Paula

; Toma, Ricardo K.

; Correa-Silva, Simone

; Sieczkowska, Sofia M.

; Imamura, Marta

; Forsait, Silvana

; Santos, Vera A.

; Zheng, Yingying

Clinics

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2021, Volumen 76 elocation e3511

Resumen: En | Texto: En | PDF: En

OBJECTIVES: To prospectively evaluate demographic, anthropometric and health-related quality of life (HRQoL) in pediatric patients with laboratory-confirmed coronavirus disease 2019 (COVID-19) METHODS: This was a longitudinal observational study of surviving pediatric post-COVID-19 patients (n=53) and pediatric subjects without laboratory-confirmed COVID-19 included as controls (n=52) was performed. RESULTS: The median duration between COVID-19 diagnosis (n=53) and follow-up was 4.4 months (0.8-10.7). Twenty-three of 53 (43%) patients reported at least one persistent symptom at the longitudinal follow-up visit and 12/53 (23%) had long COVID-19, with at least one symptom lasting for >12 weeks. The most frequently reported symptoms at the longitudinal follow-up visit were headache (19%), severe recurrent headache (9%), tiredness (9%), dyspnea (8%), and concentration difficulty (4%). At the longitudinal follow-up visit, the frequencies of anemia (11% versus 0%, p=0.030), lymphopenia (42% versus 18%, p=0.020), C-reactive protein level of >30 mg/L (35% versus 0%, p=0.0001), and D-dimer level of >1000 ng/mL (43% versus 6%, p=0.0004) significantly reduced compared with baseline values. Chest X-ray abnormalities (11% versus 2%, p=0.178) and cardiac alterations on echocardiogram (33% versus 22%, p=0.462) were similar at both visits. Comparison of characteristic data between patients with COVID-19 at the longitudinal follow-up visit and controls showed similar age (p=0.962), proportion of male sex (p=0.907), ethnicity (p=0.566), family minimum monthly wage (p=0.664), body mass index (p=0.601), and pediatric pre-existing chronic conditions (p=1.000). The Pediatric Quality of Live Inventory 4.0 scores, median physical score (69 [0-100] versus 81 [34-100], p=0.012), and school score (60 [15-100] versus 70 [15-95], p=0.028) were significantly lower in pediatric patients with COVID-19 at the longitudinal follow-up visit than in controls. CONCLUSIONS: Pediatric patients with COVID-19 showed a longitudinal impact on HRQoL parameters, particularly in physical/school domains, reinforcing the need for a prospective multidisciplinary approach for these patients. These data highlight the importance of closer monitoring of children and adolescents by the clinical team after COVID-19.

https://doi.org/10.6061/clinics/2021/e3511

Clinical, laboratory, ultrasonographic, and anatomopathological aspects of 30 cases of traumatic reticulosplenitis in cattle
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Silva, Tatiane V.

; Cajueiro, Jobson Filipe P.

; Silva, Nivan Antônio A.

; Souto, Rodolfo José C.

; Coutinho, Luiz T.

; Mendonça, Carla L.

; Afonso, José Augusto B.

; Miranda Neto, Eldinê G.

Pesquisa Veterinária Brasileira

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Sep 2020, Volumen 40 Nº 9 Paginas 669 - 676

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: A ingestão de corpos estranho de origem metálica e/ou pontiagudos desencadeia em bovinos, quadros de Reticuloperitonite Traumática e suas sequelas. Dentre as quais podemos destacar a retículo esplenite traumática cuja letalidade é elevada, embora a mesma apresente uma baixa frequência na clínica de ruminantes. Portanto, baseado na escassez de informações sobre esta enfermidade, este trabalho teve por objetivo avaliar os achados clínicos, laboratoriais, ultrassonográficos e anatomopatológicos de 30 bovinos adultos diagnosticados com retículo esplenite traumática. Os achados clínicos, ultrassonográfico e anatomopatológico foram analisados por meio de estatística descritiva, e os dados laboratoriais foram avaliados utilizando-se as medidas de tendência central. Clinicamente os animais apresentaram desidratação e alterações no comportamento, apetite e na motilidade ruminal. Os achados hematológicos revelaram leucocitose (37077.17±25004.59cell/μL) por neutrofilia com desvio à esquerda regenerativo e hiperfibrinogenemia (1130±364.98mg/dL). O exame ultrassonográfico possibilitou a visualização de filamentos móveis e ecogênicos que corresponderam à presença de aderências fibrinosas, observou-se também, deslocamento do retículo e irregularidade no seu contorno além das alterações na quantidade, padrão e amplitude das contrações reticulares. Permitiu ainda, a constatação de alterações esplênicas como abscessos que foram caracterizados como estruturas circulares de variados tamanhos delimitada por capsula contendo no seu interior conteúdo de ecogenicidade variável. Trombose da veia esplênica e dobramento do baço. Os resultados obtidos nesse trabalho, indicaram que a retículo esplenite traumática causa sinais clínicos inespecíficos, severas alterações laboratoriais e principalmente que a ultrassonografia é um método eficiente para o diagnóstico dessa enfermidade uma vez que as lesões anatomopatológicas confirmaram os achados ultrassonográficos.

ABSTRACT: Ingestion of metallic and/or sharp foreign bodies triggers cases of traumatic reticuloperitonitis and its sequelae in cattle. Among these sequelae, we can highlight traumatic reticulosplenitis, that has high mortality, although its frequency in the ruminant medicine is low. Therefore, based on the scarcity of information on this disease, the current study aimed to evaluate the clinical, laboratory, ultrasonographic, and pathological findings of 30 adult cattle diagnosed with traumatic reticulosplenitis. Clinical, ultrasound, and anatomopathological findings were analyzed using descriptive statistics and laboratory data were evaluated using measures of central tendency. Clinically the animals presented dehydration and alterations in behavior, appetite, and ruminal motility. Hematological findings revealed neutrophilic leukocytosis (37077.17±25004.59cell/μL) with regenerative left shift and hyperfibrinogenemia (1130±364.98mg/dL). The ultrasound examination enabled visualization of mobile and echogenic filaments that corresponded to the presence of fibrin adhesions. Displacement of the reticulum and irregularity in its contour, as well as alterations in the quantity, pattern, and amplitude of reticular contractions were also observed. Splenic alterations such as abscesses were found, characterized as circular structures of varying sizes delimited by capsules containing variable echogenicity. Splenic vein thrombosis and spleen folding were also observed. The results obtained in the current study indicated that traumatic reticulosplenitis causes nonspecific clinical signs, severe laboratory alterations and, mainly, that ultrasound is an efficient method for the diagnosis of this disease, since the anatomopathological lesions confirmed the ultrasound findings.

https://doi.org/10.1590/1678-5150-pvb-6743 570 descargas

German Aortic Valve Score in Risk Assessment for Surgical Aortic Valve Replacement in a Brazilian Center
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Rayol, Sérgio C.

; Sá, Michel Pompeu B. O.

; Cavalcanti, Luiz Rafael P.

; Diniz, Roberto G. S.

; Perazzo, Álvaro M.

; Escorel Neto, Antônio C. A.

; Zhigalov, Konstantin

; Ruhparwar, Arjang

; Weymann, Alexander

; Lima, Ricardo C.

Brazilian Journal of Cardiovascular Surgery

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Abr 2020, Volumen 35 Nº 2 Paginas 141 - 144

Resumen: En | Texto: En | PDF: En

Abstract Objective: To test the German Aortic Valve (GAV) score at our university hospital in patients undergoing isolated aortic valve replacement (AVR). Methods: A total of 224 patients who underwent isolated conventional AVR between January 2015 and December 2018 were included. Patients with concomitant procedures and transcatheter aortic valve implantation were excluded. Patients’ data were collected and analyzed retrospectively. Patients’ risk scores were calculated according to criteria described by GAV score. Sensitivity, specificity, and accuracy (area under the ROC curve [AUC]) were also calculated. The calibration of the model was tested by the Hosmer-Lemeshow method. Results: The mortality rate was 8.04% (18 patients). The patients’ mean age was 58.2±19.3 years and 25% of them were female (56 patients). Mean GAV score was 1.73±5.86 (min: 0.0; max: 3.53). The GAV score showed excellent discriminative capacity (AUC 0.925, 95% confidence interval 0.882-0.956; P<0.001). The cutoff “1.8” turned out to be the best discriminatory point with the best combination of sensitivity (88.9%) and specificity (75.7%) to predict operative death. Hosmer-Lemeshow method revealed a P-value of 0.687, confirming a good calibration of the model. Conclusion: The GAV score applies to our population with high predictive accuracy.

https://doi.org/10.21470/1678-9741-2019-0373 186 descargas

Arsenic Speciation in Fish and Rice by HPLC-ICP-MS Using Salt Gradient Elution
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Nogueira, Raquel

; Melo, Evyson A.

; Figueiredo, Jefferson L. C.

; Santos, Jéssica J.

; Nascimento Neto, Antonio P. do

Journal of the Brazilian Chemical Society

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Ago 2018, Volumen 29 Nº 8 Paginas 1593 - 1600

Resumen: En | Texto: En | PDF: En

This paper presents arsenic speciation studies by high-performance liquid chromatography with inductively coupled plasma-mass spectrometer (HPLC-ICP-MS) using a chromatography column containing a strong anion exchange stationary phase (Agilent Bio SAX G3154A/101) for determination of arsenobetaine (AsB), dimethylarsinic acid (DMA), AsIII, monomethylarsonic acid (MMA), and AsV. The best separation was achieved in 15 min, using a 4 min isocratic step with 1 mM (NH4)2HPO4 pH 10.2, followed by a 2 min salt gradient from 1 to 10 mM, which was kept for 4 min. Compared to the Food and Drug Administration (FDA) method for rice using the PRP-X-100 column, the Bio SAX method was quicker, provided at least equal separation of AsB from the next peak, and led to more symmetrical peaks. The linearity between 0.5 and 60 µg L-1 was shown. The method was tested with 10 µg L-1 fortified rice and fish samples extracted in ultrasound using methanol/water 1:1, and recoveries between 40 and 90% were obtained.

https://doi.org/10.21577/0103-5053.20180091 1991 descargas

Controversial issues in the management of hyperprolactinemia and prolactinomas – An overview by the Neuroendocrinology Department of the Brazilian Society of Endocrinology and Metabolism
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Vilar, Lucio

; Abucham, Julio

; Albuquerque, José Luciano

; Araujo, Luiz Antônio

; Azevedo, Monalisa F.

; Boguszewski, Cesar Luiz

; Casulari, Luiz Augusto

; Cunha Neto, Malebranche B. C.

; Czepielewski, Mauro A.

; Duarte, Felipe H. G.

; Faria, Manuel dos S.

; Gadelha, Monica R.

; Garmes, Heraldo M.

; Glezer, Andrea

; Gurgel, Maria Helane

; Jallad, Raquel S.

; Martins, Manoel

; Miranda, Paulo A. C.

; Montenegro, Renan M.

; Musolino, Nina R. C.

; Naves, Luciana A.

; Ribeiro-Oliveira Júnior, Antônio

; Silva, Cíntia M. S.

; Viecceli, Camila

; Bronstein, Marcello D.

Archives of Endocrinology and Metabolism

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Mar 2018, Volumen 62 Nº 2 Paginas 236 - 263

Resumen: En | Texto: En | PDF: En

ABSTRACT Prolactinomas are the most common pituitary adenomas (approximately 40% of cases), and they represent an important cause of hypogonadism and infertility in both sexes. The magnitude of prolactin (PRL) elevation can be useful in determining the etiology of hyperprolactinemia. Indeed, PRL levels > 250 ng/mL are highly suggestive of the presence of a prolactinoma. In contrast, most patients with stalk dysfunction, drug-induced hyperprolactinemia or systemic diseases present with PRL levels < 100 ng/mL. However, exceptions to these rules are not rare. On the other hand, among patients with macroprolactinomas (MACs), artificially low PRL levels may result from the so-called “hook effect”. Patients harboring cystic MACs may also present with a mild PRL elevation. The screening for macroprolactin is mostly indicated for asymptomatic patients and those with apparent idiopathic hyperprolactinemia. Dopamine agonists (DAs) are the treatment of choice for prolactinomas, particularly cabergoline, which is more effective and better tolerated than bromocriptine. After 2 years of successful treatment, DA withdrawal should be considered in all cases of microprolactinomas and in selected cases of MACs. In this publication, the goal of the Neuroendocrinology Department of the Brazilian Society of Endocrinology and Metabolism (SBEM) is to provide a review of the diagnosis and treatment of hyperprolactinemia and prolactinomas, emphasizing controversial issues regarding these topics. This review is based on data published in the literature and the authors' experience.

https://doi.org/10.20945/2359-3997000000032 36158 descargas

10.

A review of Cushing's disease treatment by the Department of Neuroendocrinology of the Brazilian Society of Endocrinology and Metabolism
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Machado, Márcio Carlos

; Fragoso, Maria Candida Barisson Vilares

; Moreira, Ayrton Custódio

; Boguszewski, César Luiz

; Vieira Neto, Leonardo

; Naves, Luciana A.

; Vilar, Lucio

; Araújo, Luiz Antônio de

; Musolino, Nina Rosa Castro

; Miranda, Paulo Augusto C.

; Czepielewski, Mauro A.

; Gadelha, Monica R.

; Bronstein, Marcello Delano

; Ribeiro-Oliveira Jr., Antônio

Archives of Endocrinology and Metabolism

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Feb 2018, Volumen 62 Nº 1 Paginas 87 - 105

Resumen: En | Texto: En | PDF: En

ABSTRACT The treatment objectives for a patient with Cushing's disease (CD) are remission of hypercortisolism, adequate management of co-morbidities, restoration of the hypothalamic-pituitary-adrenal axis, preservation of fertility and pituitary function, and improvement of visual defects in cases of macroadenomas with suprasellar extension. Transsphenoidal pituitary surgery is the main treatment option for the majority of cases, even in macroadenomas with low probability of remission. In cases of surgical failure, another subsequent pituitary surgery might be indicated in cases with persistent tumor imaging at post surgical magnetic resonance imaging (MRI) and/or pathology analysis of adrenocorticotropic hormone-positive (ACTH+) positive pituitary adenoma in the first procedure. Medical treatment, radiotherapy and adrenalectomy are the other options when transsphenoidal pituitary surgery fails. There are several options of medical treatment, although cabergoline and ketoconazole are the most commonly used alone or in combination. Novel treatments are also addressed in this review. Different therapeutic approaches are frequently needed on an individual basis, both before and, particularly, after surgery, and they should be individualized. The objective of the present review is to provide the necessary information to achieve a more effective treatment for CD. It is recommended that patients with CD be followed at tertiary care centers with experience in treating this condition.

https://doi.org/10.20945/2359-3997000000014 5266 descargas

11.

Morphogenesis of the rhea (Rhea americana) respiratory system in different embryonic and foetal stages
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Sousa, Renata P.

; Monteiro, Hatawa M. de A.

; Bezerra, Dayseanny de O.

; Soares, Leticya L. da S.

; Assis Neto, Antônio C.

; Rici, Rose E.G.

; Conde Júnior, Aírton M.

; Carvalho, Maria A.M. de

Pesquisa Veterinária Brasileira

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Ene 2018, Volumen 38 Nº 1 Paginas 154 - 166

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: A ema (Rhea americana) representa importante espécie silvestre que vem se destacando na pecuaria nacional e internacional. Esta pesquisa objetiva analisar o desenvolvimento embrionário-fetal, em diferentes fases, do sistema respiratório de emas. Vinte e três embriões e fetos foram eutanasiados, fixados e dissecados. Fragmentos do sistema respiratório: cavidade nasal, laringe, traqueia, siringe, brônquios e pulmões, foram coletados e processados para estudos por meio de microscopia de luz e microscopia eletrônica de varredura. A cavidade nasal apresentou, nas primeiras fases de desenvolvimento, epitélio estratificado cúbico. A laringe exibiu epitélio respiratório típico entre 27 e 31 dias. A traqueia aos 15 dias apresentou início de formação da cartilagem hialina. Na siringe a túnica mucosa de fetos de 18 dias e formada por epitélio estratificado ciliado na região bronquial. Os brônquios principais apresentavam epitélio estratificado ciliado com células caliciformes na região siringeal. No pulmão, o estágio parabronquial apresentou numerosos parabrônquios entre 15 a 21 dias. Este estudo permitiu a identificação de eventos normais que ocorrem durante o desenvolvimento do sistema respiratório de emas, importante modelo ainda não descrito. As informações geradas serão úteis para o diagnóstico de patologias que acometem este sistema orgânico, visando a melhoria dos sistemas de produção em cativeiro.

ABSTRACT: The rhea (Rhea americana) is an important wild species that has been highlighted in national and international livestock. This research aims to analyse embryo-foetal development in different phases of the respiratory system of rheas. Twenty-three embryos and foetuses were euthanized, fixed and dissected. Fragments of the respiratory system, including the nasal cavity, larynx, trachea, syrinx, bronchi and lungs, were collected and processed for studies using light and scanning electron microscopy. The nasal cavity presented cubic epithelium in the early stages of development. The larynx exhibited typical respiratory epithelium between 27 and 31 days. The trachea showed early formation of hyaline cartilage after 15 days. Syrinx in the mucous membrane of 18-day foetuses consisted of ciliated epithelium in the bronchial region. The main bronchi had ciliated epithelium with goblet cells in the syringeal region. In the lung, the parabronchial stage presented numerous parabronchi between 15 and 21 days. This study allowed the identification of normal events that occur during the development of the rhea respiratory system, an important model that has not previously been described. The information generated here will be useful for the diagnosis of pathologies that affect this organic system, aimed at improving captive production systems.

https://doi.org/10.1590/1678-5150-pvb-5310 1776 descargas

12.

Performance of irrigated green corn cultivars in different plant populations at sowing
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Soares Neto, José C.

; Santos Neto, Antônio L. dos

; Santos, Wellington M. dos

; Barros, Daline T. S.

; Souza, Ademária A. de

; Albuquerque Neto, João C. de

Revista Brasileira de Engenharia Agrícola e Ambiental

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Abr 2017, Volumen 21 Nº 4 Paginas 267 - 272

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO O trabalho objetivou avaliar a produtividade de híbridos de milho-verde sob irrigação cultivados em diferentes populações de plantas na semeadura. Foi conduzido na área experimental localizada no município de Arapiraca, AL, no período de novembro de 2015 a janeiro de 2016. O delineamento experimental utilizado foi em blocos casualizados, em esquema fatorial 2 x 5, com quatro repetições. Foram analisados dois híbridos, duplo e simples, indicados para milho verde (AG 1051 e BM 3061) e cinco espaçamentos entre plantas na semeadura (15,0; 17,5; 20,0; 22,5 e 25 cm). Foram avaliadas as características: taxa fotossintética (TF), comprimento de espigas com palha (CECP) e sem palha (CESP), peso de espigas com palhas (PECP) e sem palhas (PESP), porcentagem de espigar comerciais (%EC) e peso da palha (PP). Os híbridos duplo e simples, 1051 e BM 3061, apresentam espigas verdes com padrão comercial. A cultivar BM 3061 possui melhor desempenho para a maioria das características relacionadas ao desempenho da cultura (TF, CECP, CESP, PP, PECP, PESP, %EC). O espaçamento de 17,5 cm entre plantas na semeadura mostrou-se mais indicado para o cultivo do milho-verde irrigado.

ABSTRACT This study aimed to evaluate the yield of green corn hybrids grown under irrigation in different plant populations at sowing. The assay was carried out in the experimental area located in the city of Arapiraca, Alagoas State, Brazil, from November 2015 to January 2016. A randomized complete block design (RCBD) was used, in a 2 x 5 factorial scheme with four replicates. A double- and a single-cross hybrid (AG 1051 and BM 3061), which are suitable for green corn production, were cultivated in five spacings between plants at sowing (15.0, 17.5, 20.0, 22.5 and 25.0 cm). The characteristics photosynthetic rate (PR), ear length with rusk (HEL) and without husk (UEL), husked ear weight (HEW), unhusked ear weight (UEW), percentage of marketable ears (%ME) and weight of husk (HW) were evaluated. The double- and single-cross hybrids AG 1051 and BM 3061 showed green ears with commercial standard. The cultivar BM 3061 showed the best results for most of the characteristics related to the performance of green corn (PR, HEL, UEL, UEW, HEW, %ME). The spacing of 17.5 cm between plants at sowing was the most indicated for irrigated green corn cultivation.

https://doi.org/10.1590/1807-1929/agriambi.v21n4p267-272 1042 descargas

13.

A review on the diagnosis and treatment of patients with clinically nonfunctioning pituitary adenoma by the Neuroendocrinology Department of the Brazilian Society of Endocrinology and Metabolism
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Vieira Neto, Leonardo

; Boguszewski, Cesar L.

; Araújo, Luiz Antônio de

; Bronstein, Marcello D.

; Miranda, Paulo Augusto C.

; Musolino, Nina R. de C.

; Naves, Luciana A.

; Vilar, Lucio

; Ribeiro-Oliveira Júnior, Antônio

; Gadelha, Mônica R.

Archives of Endocrinology and Metabolism

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Ago 2016, Volumen 60 Nº 4 Paginas 374 - 390

Resumen: En | Texto: En | PDF: En

ABSTRACT Clinically nonfunctioning pituitary adenomas (NFPA) are the most common pituitary tumors after prolactinomas. The absence of clinical symptoms of hormonal hypersecretion can contribute to the late diagnosis of the disease. Thus, the majority of patients seek medical attention for signs and symptoms resulting from mass effect, such as neuro-ophthalmologic symptoms and hypopituitarism. Other presentations include pituitary apoplexy or an incidental finding on imaging studies. Mass effect and hypopituitarism impose high morbidity and mortality. However, early diagnosis and effective treatment minimizes morbidity and mortality. In this publication, the goal of the Neuroendocrinology Department of the Brazilian Society of Endocrinology and Metabolism is to provide a review of the diagnosis and treatment of patients with NFPA, emphasizing that the treatment should be performed in reference centers. This review is based on data published in the literature and the authors’ experience. Arch Endocrinol Metab. 2016;60(4):374-90

https://doi.org/10.1590/2359-3997000000179 7830 descargas

14.

Recommendations of the Neuroendocrinology Department of the Brazilian Society of Endocrinology and Metabolism for the diagnosis of Cushing’s disease in Brazil
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Machado, Márcio Carlos

; Fragoso, Maria Candida Barisson Vilares

; Moreira, Ayrton Custódio

; Boguszewski, César Luiz

; Vieira Neto, Leonardo

; Naves, Luciana A.

; Vilar, Lucio

; Araújo, Luiz Antônio de

; Czepielewski, Mauro A.

; Gadelha, Monica R.

; Musolino, Nina Rosa Castro

; Miranda, Paulo Augusto C.

; Bronstein, Marcello Delano

; Ribeiro-Oliveira Jr., Antônio

Archives of Endocrinology and Metabolism

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Jun 2016, Volumen 60 Nº 3 Paginas 267 - 286

Resumen: En | Texto: En | PDF: En

ABSTRACT Although it is a rare condition, the accurate diagnosis and treatment of Cushing’s disease is important due to its higher morbidity and mortality compared to the general population, which is attributed to cardiovascular diseases, diabetes mellitus and infections. Screening for hypercortisolism is recommended for patients who present multiple and progressive clinical signs and symptoms, especially those who are considered to be more specific to Cushing’s syndrome, abnormal findings relative to age (e.g., spinal osteoporosis and high blood pressure in young patients), weight gain associated with reduced growth rate in the pediatric population and for those with adrenal incidentalomas. Routine screening is not recommended for other groups of patients, such as those with obesity or diabetes mellitus. Magnetic resonance imaging (MRI) of the pituitary, the corticotropin-releasing hormone (CRH) test and the high-dose dexamethasone suppression test are the main tests for the differential diagnosis of ACTH-dependent Cushing’s syndrome. Bilateral and simultaneous petrosal sinus sampling is the gold standard method and is performed when the triad of initial tests is inconclusive, doubtful or conflicting. The aim of this article is to provide information on the early detection and establishment of a proper diagnosis of Cushing’s disease, recommending follow-up of these patients at experienced referral centers. Arch Endocrinol Metab. 2016;60(3):267-86

https://doi.org/10.1590/2359-3997000000174 6344 descargas

15.

Noninvasive evaluation of respiratory muscles in pre-clinical model of Duchenne Muscular Dystrophy
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Oliveira, Daniela M.

; Hagen, Stefano C.

; Santos, Amilton C.

; Miglino, Maria A.

; Assis Neto, Antônio C.

Pesquisa Veterinária Brasileira

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Abr 2016, Volumen 36 Nº 4 Paginas 290 - 296

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Uma vez que, a insuficiência respiratória é a principal causa de morte em pacientes afetados pela Distrofia Muscular de Duchenne (DMD), o presente estudo avaliou as condições clínicas cardiorrespiratórias, o movimento dos músculos intercostais, a mobilidade diafragmática, os ciclos respiratórios e a expansão da cavidade torácica em cães Golden Retriever com Distrofia Muscular (GRMD) por ultrassonografia (US) a fim de estabelecer um novo método não invasivo para avaliar os parâmetros de avaliação clínica de doenças respiratórias de modelos experimentais afetados por DMD. Um grupo controle constituído por cães da mesma raça e espécie, mas não afetados pela distrofia muscular também foram utilizados neste estudo. Os resultados mostraram que os movimentos de inspiração, expiração e platô (mobilidade do diafragma) foram menores no grupo afetado. A fase de platô no grupo afetado foi praticamente inexistente e mostrou que o diafragma destes animais permaneceu em constante movimento. A frequência respiratória atingiu 15,5 por minuto para o grupo afetado e 26,93 para o controle. Movimento de expiração e inspiração dos músculos intercostais atingiu 8,99 milímetros e 8,79 milímetros, respectivamente para o grupo controle e 7,42 milímetros e 7,40 milímetros, respectivamente para o grupo afetado. A metodologia utilizada nesta análise foi viável para o acompanhamento e avaliação do modelo respiratória em modelo GRMD e pode ser adaptado para outros modelos experimentais de distrofia muscular.

Abstract Since respiratory insufficiency is the main cause of death in patients affected by Duchenne Muscular Dystrophy (DMD), the present study aims at establishing a new non-invasive method to evaluate the clinical parameters of respiratory conditions of experimental models affected by DMD. With this purpose in mind, we evaluated the cardiorespiratory clinical conditions, the changes in the intercostal muscles, the diaphragmatic mobility, and the respiratory cycles in Golden Retriever Muscular Dystrophy (GRMD) employing ultrasonography (US). A control group consisting of dogs of the same race, but not affected by muscular dystrophy, were used in this study. The results showed that inspiration, expiration and plateau movements (diaphragm mobility) were lower in the affected group. Plateau phase in the affected group was practically non-existent and showed that the diaphragm remained in constant motion. Respiratory rate reached 15.5 per minute for affected group and 26.93 per minute for the control group. Expiration and inspiration movements of intercostal muscles reached 8.99mm and 8.79mm, respectively, for control group and 7.42mm and 7.40mm, respectively, for affected group. Methodology used in the present analysis proved to be viable for the follow-up and evaluation of the respiratory model in GRMD and may be adapted to other muscular dystrophy experimental models.

https://doi.org/10.1590/S0100-736X2016000400007 1569 descargas

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