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Abstract Background: Psoriasis is associated with several comorbidities and its association with thyroid abnormality has been hypothesized. Objective: To assess the prevalence of thyroid abnormality in Brazilian patients with psoriasis and to analyze its association with severity, presence of psoriatic arthritis and immunobiological treatment. Additionally, to compare results with literature as a control. Methods: In this observational study, clinical and laboratory data of patients followed from January 2018 to December 2019 were analyzed. Thyroid abnormality was assessed through the current history of thyroid disease and laboratory tests – thyrotropin (TSH), free thyroxine (FT4), antithyroid peroxidase (anti-TPO) and antithyroglobulin (anti-TG) antibodies. Patients were classified according to psoriasis severity – Psoriasis Area and Severity Index (PASI), presence of psoriatic arthritis, and current treatment. Subsequently, the results were compared with a control group selected from the literature review. Results: Of the 250 included patients, 161 were eligible. The prevalence of thyroid abnormality was 28.57% and of hypothyroidism, 14.91%. The mean age was 55 years and the median PASI was 2.2. There was no association between thyroid abnormality and PASI (p = 0.8), presence of psoriatic arthritis (p = 0.87), or use of immunobiological therapy (p = 0.13). The literature control group included 6,227 patients and there was a statistically significant difference for the hypothyroidism variable (p < 0.0001).

https://doi.org/10.1016/j.abd.2022.12.007

Noteworthy records of the ticks Ornithodoros rostratus and Amblyomma sculptum parasitizing Pteronura brasiliensis in the central-western region of Brazil, with pathogen investigation notes
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Barros-Battesti, Darci Moraes

; André, Marcos Rogério

; Hoppe, Estevam Guilherme Lux

; Seron, Gustavo

; Bassini-Silva, Ricardo

; Calchi, Ana Claúdia

; Andrade, Lívia Oliveira

; Perin, Patrícia Parreira

; Martins, Thiago Fernandes

; Castro-Santiago, Ana Carolina

; Leuchtenberger, Caroline

; Almeida, Samara

; Foerster, Nathalie

; Furtado, Mariana

; de Castro Jacinavicius, Fernando

Revista Brasileira de Parasitologia Veterinária

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2024, Volumen 33 Nº 1 elocation e014523

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Um macho de Pteronura brasiliensis (Carnivora: Mustelidae) foi encontrado morto às margens do Rio Negro, no Pantanal do estado de Mato Grosso do Sul, município de Aquidauana. Dois carrapatos encontrados aderidos à sua pele foram identificados, morfologicamente, como uma ninfa de segundo ínstar de Ornithodoros rostratus (Argasidae) e um macho de Amblyomma sculptum (Ixodidae). Para complementar a identificação morfológica, esses espécimes de carrapatos foram submetidos à extração de DNA e testados por meio de ensaios de PCR, para confirmar a identidade molecular dos espécimes. Além disso, os extraídos dos carrapatos foram testados e revelaram-se negativos nos ensaios de PCR para todos os patógenos testados. Também foram examinados 30 lotes, compostos por 174 indivíduos de O. rostratus, depositados na Coleção Acari do Instituto Butantan, e encontramos material de quatro estados brasileiros, incluindo-se um lote contendo 2 machos e 2 fêmeas de Aquidauana, estado de Mato Grosso do Sul (MS), coletado do solo. Este foi, portanto, o primeiro registro de O. rostratus parasitando P. brasiliensis e o primeiro registro de localidade (Aquidauana). Da mesma forma, A. sculptum é comumente encontrado no Pantanal e relatado aqui, pela segunda vez, parasitando a ariranha, hospedeiro pouco estudado em relação a ectoparasitas.

Abstract A male of Pteronura brasiliensis (Carnivora: Mustelidae) was found dead on the banks of the Rio Negro, in the Pantanal wetlands of Mato Grosso do Sul state, Aquidauana municipality. Two ticks found attached to its skin were morphologically identified as a second-instar nymph of Ornithodoros rostratus (Argasidae) and a male of Amblyomma sculptum (Ixodidae). In order to complement the morphological identification, these tick specimens were subjected to DNA extraction, and tested using PCR assays to confirm the molecular identity the specimens. Also, the tick DNA samples were tested and were negative in the PCR assays for all the pathogens tested. We also examined 30 batches, consisting of 174 individuals of O. rostratus deposited in the Acari Collection of the Butantan Institute, and we found material from four Brazilian states, including one batch containing 2 males and 2 females from Aquidauana, of Mato Grosso do Sul state, collected from the soil. This was therefore the first record of O. rostratus parasitizing P. brasiliensis and the first locality record (Aquidauana). Likewise, A. sculptum is commonly found in the Pantanal and is reported here for the second time parasitizing the giant otter, which is a host little studied regarding the ectoparasites.

https://doi.org/10.1590/s1984-29612024003

Apoio social na prática de atividade física de trabalhadores
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Almeida, Letícia Yamawaka de

; Almeida, Janaína Cristina Pasquini de

; Oliveira, Jaqueline Lemos de

; Belchior, Débora Vieira

; Zanetti, Ana Carolina Guidorizzi

; Souza, Jacqueline de

Acta Paulista de Enfermagem

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2024, Volumen 37 elocation eAPE01521

Resumen: En Es Pt | Texto: En Es Pt | PDF: En | PDF: Es | PDF: Pt

Abstract Objective To analyze the role of formal and informal social support in the practice of physical activity during workers’ leisure time. Methods A cross-sectional study carried out with 395 technical administrative workers at a public university. Data were collected using sociodemographic questionnaire, Baecke Habitual Physical Activity Questionnaire and Physical Activity and Social Support Scale. Data were analyzed using descriptive statistics, Student’s t test, Cohen’s d test and Analysis of Variance. Results Participants who reported some type of support for physical activity in their leisure time (92.2%) had higher means in the physical activity score. Institutional support (58.0%) had a prominent role as a potential influencer compared to support from immediate superiors (14.2%), although they had a certain differential among maintenance workers. Conclusion Social support plays an important role in promoting physical activity in workers’ leisure time. Institutional initiatives to encourage active behavior in the work environment need to be aligned with encouragement by immediate superiors so that they are more effective.

Resumen Objetivo Analizar la función del apoyo social formal e informal en la práctica de actividad física durante el tiempo libre de trabajadores. Métodos Estudio transversal realizado con 395 trabajadores técnicos administrativos de una universidad pública. Los datos se recopilaron utilizando un cuestionario sociodemográfico, el Cuestionario de Actividad Física Habitual de Baecke y la Escala de Apoyo Social para la Práctica de Actividad Física. Los datos se analizaron mediante estadística descriptiva, test-T de Student, prueba d de Cohen y análisis de varianza. Resultados Los participantes que relataron algún tipo de apoyo para actividad física en el tiempo libre (92,2 %) presentaron mayores promedios en el puntaje de actividad física. El apoyo institucional (58,0 %) tuvo una función destacada como posible influenciador en comparación con el apoyo de jefes directos (14,2 %), aunque estos últimos hayan ejercido cierto diferencial entre los trabajadores de mantenimiento. Conclusión El apoyo social tiene una función importante en la promoción de la actividad física en el tiempo libre de trabajadores. Las iniciativas institucionales de estímulo a un comportamiento activo en el ambiente de trabajo necesitan estar alineadas con el incentivo por parte de los superiores inmediatos para que sean más efectivas.

Resumo Objetivo Analisar a função dos apoios sociais formal e informal na prática de atividade física durante o tempo livre de trabalhadores. Métodos Estudo transversal realizado com 395 trabalhadores técnicos administrativos em uma universidade pública. Os dados foram coletados utilizando: questionário sociodemográfico, Questionário de Atividade Física Habitual de Baecke e Escala de Apoio Social para a prática de Atividade Física. Os dados foram analisados por meio de estatística descritiva, teste t de Student, teste d de Cohen e Análise de Variância. Resultados Os participantes que referiram algum tipo de apoio para atividade física no tempo livre (92,2%) apresentaram maiores médias no escore de atividade física. O apoio institucional (58,0%) teve função de destaque como potencial influenciador em comparação ao apoio de chefias imediatas (14,2%), embora elas tenham exercido um certo diferencial entre os trabalhadores da manutenção. Conclusão O apoio social tem função importante na promoção da atividade física no tempo livre de trabalhadores. As iniciativas institucionais de estímulo ao comportamento ativo no ambiente de trabalho carecem de alinhamento com o incentivo por parte dos superiores imediatos, para que sejam mais efetivas.

https://doi.org/10.37689/acta-ape/2024ao0000521

Effectiveness and safety of tocilizumab for COVID-19: a systematic review and meta-analysis of randomized clinical trials
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Almeida, Paula Ribeiro Lopes

; Person, Osmar Clayton

; Puga, Maria Eduarda dos Santos

; Giusti, Maria Fernanda

; Pinto, Ana Carolina Pereira Nunes

; Rocha, Aline Pereira

; Atallah, Álvaro Nagib

Sao Paulo Medical Journal

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Abr 2023, Volumen 141 Nº 2 Paginas 168 - 176

Resumen: En | Texto: En | PDF: En

ABSTRACT BACKGROUND: Tocilizumab is an anti-human interleukin 6 receptor monoclonal antibody that has been used to treat coronavirus disease 2019 (COVID-19). However, there is no consensus on its efficacy for the treatment of COVID-19. OBJECTIVE: To evaluate the effectiveness and safety of tocilizumab for treating COVID-19. DESIGN AND SETTING: Systematic Review of randomized controlled trials (RCTs), Universidade Federal de São Paulo (UNIFESP), São Paulo (SP), Brazil. METHODS: We searched MEDLINE via PubMed, EMBASE, CENTRAL, and IBECS for RCTs published up to March 2021. Two authors selected studies and assessed the risk of bias and the certainty of the evidence following Cochrane Recommendations. RESULTS: Eight RCTs with 6,139 participants were included. We were not able to find differences between using tocilizumab compared to standard care on mortality in hospitalized patients with COVID-19 (risk ratio (RR) 0.97, 95% confidence interval (CI) 0.84 to 1.13; 8 trials; 5,950 participants; low-certainty evidence). However, hospitalized patients under tocilizumab plus standard care treatment seemed to present a significantly lower risk of needing mechanical ventilation (risk ratio = 0.78; 95% CI 0.64−0.94 moderate-certainty of evidence). CONCLUSIONS: To date, the best evidence available shows no difference between using tocilizumab plus standard care compared to standard care alone for reducing mortality in patients with COVID-19. However, as a finding with a practical implication, the use of tocilizumab in association to standard care probably reduces the risk of progressing to mechanical ventilation in those patients. REGISTRATION: osf.io/qe4fs.

https://doi.org/10.1590/1516-3180.2022.0170.r1.01072022

Parental body mass index and maternal gestational weight gain associations with offspring body composition in young women from the Nutritionists’ Health Study
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Freitas, Renata Germano Borges de Oliveira Nascimento

; Vasques, Ana Carolina Junqueira

; Ribeiro, Francieli Barreiro

; Solar, Isabela

; Hanada, Alfredo Shigueo

; Barbosa, Marina Gomes

; Valente, Angélica Marques Martins

; Pititto, Bianca de Almeida

; Eshriqui, Ilana

; Lopes, Tito Lívio da Cunha

; Geloneze, Bruno

; Ferreira, Sandra Roberta Gouvea

Archives of Endocrinology and Metabolism

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Feb 2023, Volumen 67 Nº 1 Paginas 101 - 110

Resumen: En | Texto: En | PDF: En

ABSTRACT Objective: Intrauterine environment can induce fetal metabolic programming that predisposes to adiposity-related chronic diseases in its lifespan. We examined the associations of parental nutritional status and gestational weight gain with offspring body composition in early adulthood. Materials and methods: This is cross-sectional analysis of female participants of the NutriHS who were submitted to questionnaires, clinical examinations and body composition assessed by DXA. Association of pre-conception parental BMI and maternal gestational weight gain (exposures) with body composition measurements (outcomes) were analyzed using multiple linear models adjusted for Directed Acyclic Graphs-based covariables (maternal and paternal educational level, maternal age, and tobacco, alcohol and/or drugs use). The sample included 124 women (median 28 (24-31) years) with a mean BMI of 25.4 ± 4.7 kg/m2. Results: No association between previous paternal BMI and offspring’s body composition was detected. In the fully adjusted linear regression model, maternal BMI was associated with offspring’s total lean mass (β = 0.66, p = 0.001), appendicular skeletal muscle mass index (ASMI) (β = 0.11, p = 0.003) and fat mass index (FMI) (β = 0.03, p = 0.039). Gestational weight gain was associated with increased offspring’s BMI (OR 1.12 [95% CI 1.02-1.20], p = 0.01). The linear regression model adjusted for maternal age and maternal and paternal education levels showed associations of gestational weight gain with offspring’s ASMI (β = 0.42, p = 0.046), FMI (β = 0.22, p = 0.005) and android-to-gynoid fat ratio (β = 0.09, p = 0.035). Conclusion: Our findings suggest that preconception maternal BMI could influence lean mass and general adiposity of young adult female offspring and that gestational weight gain could be useful for predicting centrally distributed adiposity.

https://doi.org/10.20945/2359-3997000000516

Low serum uric acid levels and levodopa-induced dyskinesia in Parkinson's disease
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Soares, Nayron Medeiros

; Pereira, Gabriela Magalhães

; Dutra, Ana Carolina Leonardi

; Artigas, Nathalie Ribeiro

; Krimberg, Júlia Schneider

; Monticelli, Bruno Elkfury

; Schumacher-Schuh, Artur Francisco

; Almeida, Rosa Maria Martins de

; Rieder, Carlos Roberto de Mello

Arquivos de Neuro-Psiquiatria

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Ene 2023, Volumen 81 Nº 1 Paginas 40 - 46

Resumen: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Background Levodopa is the most used and effective medication for motor symptoms of Parkinson disease (PD), its long-term use is associated with the appearance of levodopa-induced dyskinesia (LID). Uric acid (UA) is believed to play an important neuroprotective role in PD. Objective To investigate if serum UA levels are related with the presence of LIDs in PD patients. Also, we investigated the associations among UA levels and clinical features of PD. Methods We enrolled 81 PD patients (dyskinesia = 48; no dyskinesia = 33) in the present study. A blood sample was collected to evaluate serum UA levels, clinical evaluation included the following instruments: Montreal Cognitive Assessment (MoCA), Beck Depression Inventory II (BDI-II), MDS-Unified Parkinson's Disease Rating Scale (MDS-UPDRS), Hoehn and Yahr (HY), and the sub-item 4.1 of MDS-UPDRS IV (score ≥ 1). Additional relevant clinical information was obtained by a clinical questionnaire. Results Serum UA levels were lower in the dyskinesia group when compared with the no dyskinesia group. The same result was found in the UA levels of both men and women. The multivariate analysis showed lower uric acid levels were significantly associated with having dyskinesia (odds ratio [OR] = 0.424; 95% confidence interval [CI]: 0.221-0.746; p= 0.005). Additional analysis verified that serum UA levels are inversely correlated with depressive symptoms, disease duration, MDS-UPDRS IV and time spent with dyskinesia. A positive correlation was found with age at onset of PD symptoms. Conclusions The present study provides a possible role of serum UA levels in LID present in PD patients.

Resumo Antecedentes A levodopa é a medicação mais utilizada e eficaz para os sintomas motores da doença de Parkinson (DP); seu uso a longo prazo está associado ao aparecimento de discinesia induzida por levodopa (LID). Acredita-se que o ácido úrico desempenhe um importante papel neuroprotetor na DP. Objetivo Investigar se os níveis séricos de AU estão relacionados com a presença de LID em pacientes com DP. Além disso, investigamos as associações entre os níveis de AU e as características clínicas da DP. Métodos Foram incluídos 81 pacientes com DP (discinesia = 48; sem discinesia = 33) no presente estudo. Uma amostra de sangue foi coletada para avaliar os níveis séricos de AU, a avaliação clínica incluiu os seguintes instrumentos: Avaliação Cognitiva de Montreal (MoCA), Inventário de Depressão de Beck (BDI-II), MDS-Unified Parkinson's Disease Rating Scale (MDS-UPDRS), Hoehn and Yahr (HY) e o subitem 4.1 da MDS-UPDRS IV (escore ≥ 1). Informações clínicas relevantes adicionais foram obtidas por meio de um questionário clínico. Resultados Os níveis séricos de AU foram menores no grupo com discinesia quando comparados ao grupo sem discinesia. O mesmo resultado foi encontrado nos níveis de AU de homens e mulheres. A análise multivariada mostrou que níveis mais baixos de ácido úrico foram significativamente associados a ter discinesia (odds ratio [OR] = 0,424; intervalo de confiança (IC) de 95%: 0,221-0,746; p= 0,005). Análises adicionais verificaram que os níveis séricos de AU estão inversamente correlacionados com sintomas depressivos, duração da doença, MDS-UPDRS IV e tempo gasto com discinesia. Uma correlação positiva foi encontrada com a idade de início dos sintomas da DP. Conclusões O presente estudo fornece um possível papel dos níveis séricos de AU na LID presente em pacientes com DP.

https://doi.org/10.1055/s-0043-1761294

The Contribution of Meteorological Parameters and the COVID-19 Partial Lockdown on Air Quality in Rio de Janeiro, Brazil
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Beringui, Karmel

; Justo, Elizanne P. S.

; Ventura, Luciana M. B.

; Gomes, Ruan G. S.

; Lionel-Mateus, Vinícius

; Cruz, Alex H. De La

; Almeida, Ana Carolina L. B. de

; Ramos, Michelle B.

; Suazo, Julio Angeles

; Valle, Pedro H. R.

; Gioda, Adriana

Journal of the Brazilian Chemical Society

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Ene 2023, Volumen 34 Nº 1 Paginas 69 - 82

Resumen: En | Texto: En | PDF: En

This study evaluated the pollutant levels (NO2, SO2, CO, and O3), air quality index (AQI) and the influence of meteorological variables and coronavirus disease (COVID-19) pandemic on the air quality in Rio de Janeiro. The data set used comprises periods before (March-April, 2019) and during pandemic (March-April, 2020). According to the AQI results, on most days, the air quality was ranked as “good”. Brazilian air quality standards for SO2, O3, and NO2 were not exceeded in any of the monitoring stations during partial lockdown, while CO exceeded in all periods in one site due to industrial emission. Comparing both periods, descriptive statistics for the meteorological parameters presented no differences, which suggests similar conditions. However, when evaluated week by week in 2020, weather conditions presented some differences that probably affected pollutant concentrations. The correlations between O3 and NO2 and some meteorological parameters indicate that variations in both favored ozone formation, since it is a photochemical process favored by temperature and solar radiation and that, in Rio de Janeiro, low NO2 concentrations lead to increased O3. The improvements on air quality during the partial lockdown may be attributed mainly to a reduction on emission sources rather than weather conditions.

https://doi.org/10.21577/0103-5053.20220089

Trends in consuming alcoholic beverages among adolescents and young adults of school age: sexes differences
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Mola, Rachel

; Araújo, Rodrigo Cappato de

; Barbosa, Shayra Anny

; Almeida, Layane Sá

; Pitangui, Ana Carolina Rodarti

Jornal de Pediatria

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Ene 2023, Volumen 99 Nº 1 Paginas 72 - 78

Resumen: En | Texto: En | PDF: En

Abstract Objective: To analyze the tendency of alcoholic beverage consumption among adolescents and young adults at school age according to their sexes. Methods: This is a trend research study in public schools of the municipality of Petrolina, between 2014 and 2016, with 3146 students aged between 12 and 24 years old. The instrument was constituted by socio-economic inquiry and the Youth Risk Behavior Survey. Trends were assessed using the Centers for Disease Control and Prevention recommended approach. The analyses were conducted using logistic regression, with a statistical significance of 0.05. Results: Close to 56% of the adolescents had already tried some alcoholic beverage, most of which had contact after 13 years of age. The prevalence of binge drinking ranged between 17% and 25%. The percentage of girls who tried alcohol before 13 years old remained similar over the three years (p = 0.943). The prevalence of this behavior was stabilized at around 20%. There was a trend to decrease in the prevalence of boys who reported having tried alcohol before 13 years old (p = 0.014). The percentage of boys who reported involvement in binge drinking in the past 30 days remained stable at around 20% over the years (p = 0.951 ). The girls’ data revealed a significant decrease in binge drinking (p = 0.019). Conclusions: The general analysis suggests a trend towards stabilization of consumption among boys, and an increase among girls.

https://doi.org/10.1016/j.jped.2022.06.003

Prevenção de acidentes domésticos na primeira infância: uma revisão integrativa
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Almeida, Lindynês Amorim de

; Torres, Barbara Vitória dos Santos

; Silva, Jislene dos Santos

; Silva, Rillary Caroline de Melo

; Vieira, Ana Carolina Santana

Revista Uruguaya de Enfermería (En línea)

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2023, Volumen 18 Nº 2 elocation e401

Resumen: Es En Pt | Texto: Es En Pt | PDF: Es | PDF: En | PDF: Pt

Resumen: Introducción: La primera infancia es una fase marcada por un intenso desarrollo infantil, con la mejora de las habilidades motoras, cognitivas y sensoriales. Así, los niños están expuestos a riesgos y, por lo tanto, los cuidados deben ser redoblados para prevenir accidentes, especialmente en el propio hogar. Objetivos: Identificar los riesgos a los que están expuestos los niños, así como trabajos científicos que aborden la prevención de accidentes domésticos en la primera infancia. Métodos: Se trata de una revisión integrativa de la literatura realizada en las siguientes bases de datos: LILACS, MEDLINE, BDENF y SciELO. La evaluación, del nivel de evidencia de los artículos encontrados se obtuvo por medio del método Oxford Center for Evidence-based Medicine. Resultados: Diez artículos cumplieron con los criterios de búsqueda establecidos. Los accidentes domésticos que se destacaron en los artículos fueron: caídas, quemaduras, envenenamiento y caídas de televisión. Con relación al ambiente familiar, fue notorio que la mayoría de las familias en los estudios analizados eran de bajos ingresos. Conclusión: Los padres o responsables de los niños son fundamentales para prevenir accidentes domésticos por diversas causas. Además, el papel de los profesionales de la salud es fundamental para desarrollar o potencializar programas de prevención, principalmente dirigidos a personas en situación de vulnerabilidad social o económica, con el fin de capacitar a la población en la identificación y minimización de riesgos.

Abstract: Introduction: Early childhood is a phase marked by intense child development, with improved motor, cognitive and sensory skills. Given this, children are exposed to risks and, therefore, must redouble care to prevent accidents, especially at home. Aim: To identify the risks to which children are exposed, as well as scientific works that address the prevention of domestic accidents in early childhood. Methods: This is an integrative literature review conducted in the following databases: LILACS, MEDLINE, BDENF, and SciELO. The level of evidence of the articles found was assessed using the Oxford Center for Evidence-based Medicine method. Results: Ten articles met the established search criteria. The domestic accidents that stood out in the articles were: falls, burns, poisoning, and tipping over television sets. Regarding the family environment, it was clear that most families in the analyzed studies were of low income. Conclusion: Parents or guardians of children are essential to prevent domestic accidents from various causes. In addition, the role of health professionals is necessary to develop or enhance prevention programs, mainly aimed at the socially or economically vulnerable, to train the population in identifying and minimizing risks.

Resumo: Introdução: A primeira infância é uma fase marcada por intenso desenvolvimento infantil, com o aperfeiçoamento de habilidades motoras, cognitivas e sensoriais. Em vista disso, as crianças são expostas a riscos e portanto, cuidados devem ser redobrados para prevenir acidentes, em especial no próprio lar. Objetivos: Identificar os riscos a que as crianças são expostas, bem como os trabalhos científicos que abordam a prevenção de acidentes domésticos na primeira infância. Métodos: Trata-se de uma revisão integrativa da literatura conduzida nas bases de dados: LILACS, MEDLINE, BDENF e SciELO. A avaliação, do nível de evidência dos artigos encontrados, foi obtida por meio do método Oxford Centre for Evidence-based Medicine. Resultados: Dez artigos atenderam aos critérios de busca estabelecidos. Os acidentes domésticos que se destacaram nos artigos foram: quedas, queimaduras, envenenamento e tombamento em aparelhos televisivos. Em relação ao ambiente familiar, foi notório que a maioria das famílias dos estudos analisados eram de baixa renda. Conclusão: Os pais ou responsáveis pelas crianças são essenciais para prevenir acidentes domésticos de variadas causas. Além disso, a atuação dos profissionais de saúde é primordial para desenvolver ou potencializar programas de prevenção, principalmente voltados para aqueles que são vulneráveis social ou economicamente, a fim de capacitar a população quanto à identificação e minimização dos riscos.

https://doi.org/10.33517/rue2023v18n2a4

10.

Preparation of PLGA Nanoparticles Loaded with the Anti-Infective Ctn[15-34] Peptide for Antifungal Application
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Santos, Ana Paula dos

; Oliveira, Roberta Carolina Rangel de

; Louchard, Bianca Oliveira

; Uchoa, Antônia Flávia Justino

; Ricardo, Nágila Maria Pontes Silva

; Leal, Luzia Kalyne Almeida Moreira

; Rádis-Baptista, Gandhi

; Araújo, Tamara Gonçalves de

Brazilian Archives of Biology and Technology

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2023, Volumen 66 elocation e20220775

Resumen: En | Texto: En | PDF: En

Abstract This article aims to report the preparation and optimized formulation of Ctn[15-34], a designed anti-infective peptide, with poly(lactic acid-co-glycolic acid), PLGA, nanoparticles. Ctn[15-34] is a short peptide derived from crotalicidin, a cathelicidin-related antimicrobial peptide from the venom of the South American rattlesnake (Crotalus durissus terrificus), that has arisen as a promising antifungal and microbicide agent. The PLGA nanoparticles were prepared and loaded with carboxyfluorescein (CF)-Ctn[15-34] using the double emulsion/solvent evaporation method. After a preformulation study, which tested different formulation parameters, Poly (vinyl alcohol) 87-89% hydrolyzed and sonication with Sonifier® were choosen to prepare unloaded PLGA nanoparticles by that method, resulting in smaller particle size and Polydispersity Index (PDI) values and higher zeta potential values of nanoparticles. This better condition was used to prepare CF-Ctn[15-34]-loaded PLGA nanoparticles, resulting in homogeneous and spherical nanoparticles, with an average size of 213.2 ± 2.00 nm, PDI of 0.044 ± 0.04 and zeta potential of -16.03 ± 1.20 mV. An excellent encapsulation efficiency was obtained, corresponding to 93.3 ± 0.10 %. The drug-release profile showed a rapid initial release of the peptide, approximately 27 % in the first 24 hours, followed by a sustained release for at least 16 days. Another relevant aspect in the peptide formulation is that the CF-Ctn[15-34]-loaded nanoparticles potentiated the antifungal effect against the opportunistic pathogenic yeast Cryptococcus neoformans compared with the free, in solution Ctn[15-34], in equivalent volume dosage. A preparation method of CF-Ctn[15-34]-loaded PLGA nanoparticles was established and validated, representing a successful approach to deliver CF-Ctn[15-34] for pharmaceutical applications.

https://doi.org/10.1590/1678-4324-2023220775

11.

DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes
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Mendes-Aguiar, Carolina de O

; Kitahara-Oliveira, Milene Yoko

; de Almeida, Ana Cristina Oliveira

; Pereira-Oliveira, Marcia

; de Oliveira Neto, Manoel Paes

; Pirmez, Claude

; Sampaio, Elizabeth Pereira

; Gomes-Silva, Adriano

; Da-Cruz, Alda Maria

Memórias do Instituto Oswaldo Cruz

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2023, Volumen 118 elocation e220044

Resumen: En | Texto: En | PDF: En

BACKGROUND Dendritic cells (DCs) specific intercellular adhesion molecule (ICAM)-3-grabbing non integrin receptor (DC-SIGN) binds to subgenera Leishmania promastigotes mediating its interaction with DC and neutrophils, potentially influencing the infection outcome. OBJECTIVES In this work, we investigated whether DC-SIGN receptor is expressed in cells from cutaneous leishmaniasis (CL) lesions as well as the in vitro binding pattern of Leishmania (Viannia) braziliensis (Lb) and L. (L.) amazonensis (La) promastigotes. METHODS DC-SIGN receptor was labeled by immunohistochemistry in cryopreserved CL tissue fragments. In vitro binding assay with CFSE-labeled Lb or La promastigotes and RAJI-transfecting cells expressing DC-SIGN (DC-SIGNPOS) or mock-transfected (DC-SIGNNEG) were monitored by flow cytometry at 2 h, 24 h and 48 h in co-culture. RESULTS In CL lesion infiltrate, DC-SIGNPOS cells were present in the dermis and near the epidermis. Both Lb and La bind to DC-SIGNPOS cells, while binding to DC-SIGNNEG was low. La showed precocious and higher affinity to DC-SIGNhi population than to DC-SIGNlow, while Lb binding was similar in these populations. CONCLUSION Our results demonstrate that DC-SIGN receptor is present in L. braziliensis CL lesions and interact with Lb promastigotes. Moreover, the differences in the binding pattern to Lb and La suggest DC-SIGN can influence in a difference way the intake of the parasites at the first hours after Leishmania infection. These results raise the hypothesis that DC-SIGN receptor could participate in the immunopathogenesis of American tegumentary leishmaniasis accounting for the differences in the outcome of the Leishmania spp. infection.

https://doi.org/10.1590/0074-02760220044

12.

Impact of cervical pain, neck mobility, and body mass index on teachers’ postural control
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Dias, Ana Carolina Marcotti

; Ciquinato, Daiane Soares de Almeida

; Marchiori, Luciana Lozza de Moraes

; Andraus, Rodrigo Antonio Carvalho

Revista CEFAC

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2023, Volumen 25 Nº 1 elocation e4222

Resumen: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT Purpose: to analyze the impact of neck pain, neck mobility, and body mass index on teachers’ postural control. Methods: a cross-sectional study with 54 state public school teachers, 68.5% (n = 37) being females, with a mean age of 46.5 ± 9.3 years. Data were collected with the following instruments: Craniocervical Dysfunction Index (Brazilian version), force platform in bipedal and semi-tandem stance, visual analog scale, cervical mobility index, and body mass index. Data were analyzed with nonparametric statistics and multiple linear regression; the significance level was set at p<0.05, with 95% confidence intervals. Results: teachers with neck pain and severely impaired neck mobility had greater postural control changes in the semi-tandem stance. In the bipedal stance, those with mild mobility changes and neck pain had a smaller total displacement. Obese teachers had a smaller movement amplitude in the anteroposterior and mediolateral directions. Conclusion: teachers presented with neck pain and severely impaired neck mobility had a worse postural control. Obese teachers had a smaller total amplitude in both movement directions.

RESUMO Objetivo: analisar o impacto da cervicalgia, mobilidade cervical e índice de massa corporal no controle postural de professores. Métodos: estudo transversal com 54 professores da rede estadual de ensino, com média de idade de 46,5 ± 9,3 anos, dos quais: 68,5% (n = 37) eram do sexo feminino. A coleta de dados teve como base os seguintes instrumentos: Craniocervical Dysfunction Index (versão brasileira), plataforma de força na posição bipodal e semitandem, escala visual analógica, índice de mobilidade cervical e índice de massa corporal. Para análise dos dados, foi utilizada estatística não paramétrica e análise de regressão linear múltipla, com nível de significância p<0,05 e intervalo de confiança de 95%. Resultados: os professores com cervicalgia e comprometimento severo da mobilidade cervical tiveram maior alteração do controle postural na posição semitandem. Na posição bipodal, aqueles com leve alteração da mobilidade e dor cervical apresentaram menor deslocamento total, assim como professores obesos demostraram menor amplitude de movimento nas direções anteroposterior e médio-lateral. Conclusão: os professores com cervicalgia e comprometimento severo da mobilidade cervical tiveram pior controle postural. Já os obesos apresentaram menor amplitude total em ambas as direções do movimento.

https://doi.org/10.1590/1982-0216/20232514222

13.

Cognitive evaluation in Parkinson’s disease: applying the Movement Disorder Society recommendations in a population with a low level of formal education
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Rocha, Natalia Pessoa

; Carreira, Eduarda Xavier

; Almeida Prado, Ana Carolina de

; Tavares, Fabíola

; Tavares, Mayra

; Cardoso, Francisco

; Jaeger, Antônio

; Souza, Leonardo Cruz de

; Teixeira, Antônio Lucio

Arquivos de Neuro-Psiquiatria

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2023, Volumen 81 Nº 2 Paginas 119 - 127

Resumen: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Background The diagnosis of cognitive disorders in Parkinson disease (PD) can be very challenging. Aiming at establishing uniform and reliable diagnostic procedures, the International Parkinson’s Disease and Movement Disorder Society (MDS) commissioned task forces to delineate diagnostic criteria for mild cognitive impairment (MCI) and dementia in PD. Objectives To investigate the applicability of the MDS recommendations for cognitive evaluation in a Brazilian sample of patients with PD with low levels of formal education. Methods A total of 41 patients with PD were subjected to a comprehensive neuropsychological evaluation based on tests proposed by the MDS, which included the Mini-Mental State Examination, the Mattis Dementia Rating Scale (MDRS), the Trail Making Test (TMT) parts A and B, in addition to language and memory skills assessment. Neuropsychiatric and daily functioning features were also evaluated. Spearman correlation analyses were used to evaluate the association between the scores obtained in the cognitive scales and demographic/clinical variables. Results Although none of the participants had a formal diagnosis of dementia, 50% presented some degree of cognitive impairment when considering the results of the MDRS. Of note, a noticeable number of patients was not able to complete the full neuropsychological assessment. The TMT part B was the most difficult task, being completed by only 22 participants (54%). As expected, the greater the educational level, the better the performance on the cognitive tests. Better motor function was also associated with better scores in cognition. Conclusions Adopting strict inclusion/exclusion criteria and a comprehensive clinical evaluation, we found remarkable limitations for the MDS recommendations when individuals with low educational levels are considered. A revision of the current guidelines is necessary considering differences among populations, especially related to formal education.

Resumo Antecedentes O diagnóstico de distúrbios cognitivos na doença de Parkinson (DP) pode ser muito desafiador. Com o objetivo de estabelecer procedimentos diagnósticos uniformes e confiáveis, a Sociedade Internacional da Doença de Parkinson e Distúrbios do Movimento (MDS, na sigla em inglês) encomendou forças-tarefa para delinear critérios diagnósticos para comprometimento cognitivo leve (CCL) e demência na DP. Objetivos Investigar a aplicabilidade das recomendações da MDS para avaliação cognitiva em uma amostra brasileira de pacientes com DP de baixa escolaridade. Métodos Um total de 41 pacientes com DP foram submetidos a uma avaliação neuropsicológica abrangente com base nos testes propostos pela MDS, que incluiu o Miniexame do Estado Mental, a Escala de Avaliação de Demência de Mattis (MDRS, na sigla em inglês), o teste de trilhas (TMT, na sigla em inglês) partes A e B, além da avaliação das habilidades de linguagem e memória. Características neuropsiquiátricas e de funcionamento diário também foram avaliadas. Análises de correlação de Spearman foram utilizadas para avaliar a associação entre os escores obtidos nas escalas cognitivas e variáveis demográficas/clínicas. Resultados Apesar de nenhum dos participantes ter diagnóstico formal de demência, 50% apresentaram algum grau de comprometimento cognitivo ao levar em consideração os resultados da MDRS. Vale ressaltar que um número notável de pacientes não conseguiu completar a avaliação neuropsicológica completa. A parte B do TMT foi a tarefa mais difícil, sendo realizada por apenas 22 participantes (54%). Como esperado, quanto maior o nível educacional, melhor o desempenho nos testes cognitivos. Melhor função motora também foi associada a melhores escores em cognição. Conclusões Adotando critérios rígidos de inclusão/exclusão e uma avaliação clínica abrangente, encontramos limitações marcantes para as recomendações da MDS quando considerados indivíduos com baixa escolaridade. É necessária uma revisão das diretrizes atuais considerando as diferenças entre as populações, principalmente relacionadas ao nível educacional.

https://doi.org/10.1055/s-0042-1759761

14.

Percepções do Viver com Vaginismo: Estudo por meio do Desenho-Estória com Tema
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Silva, Ana Carolina de Moraes

; Sei, Maíra Bonafé

; Vieira, Rebeca Beckner de Almeida Prado

Psico-USF

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2023, Volumen 28 Nº 2 Paginas 309 - 320

Resumen: En Es Pt | Texto: En Es Pt | PDF: En | PDF: Es | PDF: Pt

Abstract Considering that sexual dysfunction profoundly affects self-perception and quality of life, this study aims to understand the experience of living with vaginismus. This is an empirical, qualitative, exploratory research, in which nine women diagnosed with vaginismus at some point in their lives participated. Data were collected through individual semi-directed interviews and application of the drawing and story procedure with the theme “Vaginismus”. In this paper, the analysis of the drawings and stories is highlighted due to the richness of this instrument. The material was interpreted together, enabling the construction of three thematic categories: “Representations: vaginismus as a protagonist”, “The course and history of life: the different moments of vaginismus” and “Loneliness and relationships”. The results pointed to different perceptions of vaginismus according to the time of treatment, highlighting the long duration of symptoms and the arduous process of living with this disorder.

Resumen Considerando que experiencia una disfunción sexual afecta profundamente la autopercepción y la calidad de vida, este trabajo tiene como objetivo comprender la experiencia de vivir con vaginismo. Se trata de una investigación empírica, cualitativa, exploratoria, en la que participaron nueve mujeres diagnosticadas de vaginismo en algún momento de su vida. Los datos fueron recolectados a través de entrevistas individuales semidirigidas y aplicación del procedimiento de dibujo-cuento con el tema “Vaginismo”. En este trabajo se destaca el análisis de los dibujos y cuentos por la riqueza de este instrumento. El material fue interpretado en conjunto, posibilitando la construcción de tres categorías temáticas: “Representaciones: el vaginismo como protagonista”, “El curso y la historia de la vida: los diferentes momentos del vaginismo” y “La soledad y las relaciones”. Los resultados apuntaron a diferentes percepciones del vaginismo según el tiempo de tratamiento, destacando la larga duración de los síntomas y el arduo proceso de convivencia con este trastorno.

Resumo Considerando que experienciar uma disfunção sexual afeta profundamente a autopercepção e a qualidade de vida, este estudo almejou compreender a experiência de viver com vaginismo. Trata-se de uma pesquisa empírica, qualitativa, de caráter exploratório, em que participaram nove mulheres diagnosticadas em algum período da vida com vaginismo. Os dados foram coletados por meio de entrevistas semidirigidas e do procedimento de desenhos-estórias com o tema “Vaginismo”, sendo que, neste trabalho, destaca-se a análise dos desenhos e histórias devido à riqueza deste instrumento. O material foi interpretado em conjunto possibilitando a construção de três categorias temáticas: “Representações: o vaginismo como protagonista”, “O percurso e a história de vida: os diferentes momentos do vaginismo” e “A solidão e os relacionamentos”. Os resultados apontaram para diferentes percepções do vaginismo de acordo com o momento do tratamento, destacando-se a longa duração dos sintomas e o árduo processo de viver com esta disfunção.

https://doi.org/10.1590/1413-82712023280208

15.

Diretriz da SBC sobre Diagnóstico e Tratamento de Pacientes com Cardiomiopatia da Doença de Chagas – 2023
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Marin-Neto, José Antonio

; Rassi Jr, Anis

; Oliveira, Gláucia Maria Moraes

; Correia, Luís Claudio Lemos

; Ramos Júnior, Alberto Novaes

; Luquetti, Alejandro Ostermayer

; Hasslocher-Moreno, Alejandro Marcel

; Sousa, Andréa Silvestre de

; Paola, Angelo Amato Vincenzo de

; Sousa, Antônio Carlos Sobral

; Ribeiro, Antonio Luiz Pinho

; Correia Filho, Dalmo

; Souza, Dilma do Socorro Moraes de

; Cunha-Neto, Edecio

; Ramires, Felix Jose Alvarez

; Bacal, Fernando

; Nunes, Maria do Carmo Pereira

; Martinelli Filho, Martino

; Scanavacca, Maurício Ibrahim

; Saraiva, Roberto Magalhães

; Oliveira Júnior, Wilson Alves de

; Lorga-Filho, Adalberto Menezes

; Guimarães, Adriana de Jesus Benevides de Almeida

; Braga, Adriana Lopes Latado

; Oliveira, Adriana Sarmento de

; Sarabanda, Alvaro Valentim Lima

; Pinto, Ana Yecê das Neves

; Carmo, Andre Assis Lopes do

; Schmidt, Andre

; Costa, Andréa Rodrigues da

; Ianni, Barbara Maria

; Markman Filho, Brivaldo

; Rochitte, Carlos Eduardo

; Macêdo, Carolina Thé

; Mady, Charles

; Chevillard, Christophe

; Virgens, Cláudio Marcelo Bittencourt das

; Castro, Cleudson Nery de

; Britto, Constança Felicia De Paoli de Carvalho

; Pisani, Cristiano

; Rassi, Daniela do Carmo

; Sobral Filho, Dário Celestino

; Almeida, Dirceu Rodrigues de

; Bocchi, Edimar Alcides

; Mesquita, Evandro Tinoco

; Mendes, Fernanda de Souza Nogueira Sardinha

; Gondim, Francisca Tatiana Pereira

; Silva, Gilberto Marcelo Sperandio da

; Peixoto, Giselle de Lima

; Lima, Gustavo Glotz de

; Veloso, Henrique Horta

; Moreira, Henrique Turin

; Lopes, Hugo Bellotti

; Pinto, Ibraim Masciarelli Francisco

; Ferreira, João Marcos Bemfica Barbosa

; Nunes, João Paulo Silva

; Barreto-Filho, José Augusto Soares

; Saraiva, José Francisco Kerr

; Lannes-Vieira, Joseli

; Oliveira, Joselina Luzia Menezes

; Armaganijan, Luciana Vidal

; Martins, Luiz Cláudio

; Sangenis, Luiz Henrique Conde

; Barbosa, Marco Paulo Tomaz

; Almeida-Santos, Marcos Antonio

; Simões, Marcos Vinicius

; Yasuda, Maria Aparecida Shikanai

; Moreira, Maria da Consolação Vieira

; Higuchi, Maria de Lourdes

; Monteiro, Maria Rita de Cassia Costa

; Mediano, Mauro Felippe Felix

; Lima, Mayara Maia

; Oliveira, Maykon Tavares de

; Romano, Minna Moreira Dias

; Araujo, Nadjar Nitz Silva Lociks de

; Medeiros, Paulo de Tarso Jorge

; Alves, Renato Vieira

; Teixeira, Ricardo Alkmim

; Pedrosa, Roberto Coury

; Aras Junior, Roque

; Torres, Rosalia Morais

; Povoa, Rui Manoel dos Santos

; Rassi, Sergio Gabriel

; Alves, Silvia Marinho Martins

; Tavares, Suelene Brito do Nascimento

; Palmeira, Swamy Lima

; Silva Júnior, Telêmaco Luiz da

; Rodrigues, Thiago da Rocha

; Madrini Junior, Vagner

; Brant, Veruska Maia da Costa

; Dutra, Walderez Ornelas

; Dias, João Carlos Pinto

Arquivos Brasileiros de Cardiologia

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2023, Volumen 120 Nº 6 elocation e20230269

Resumen: En Pt | Texto: En Pt | PDF: En | PDF: Pt

https://doi.org/10.36660/abc.20230269

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