Abstract Astragalus argaeus is an endemic plant species that is under critical risk of extinction. Here we investigated its population census, breeding system and reproductive success to determine the main factors affecting restricted distribution of this species. According to the results from pollination experiments, A. argaeus was autogamous but benefit from the cross pollination (xenogamous) and the presence of pollinators increases fruit set. The pollen/ovule ratio of A. argaeus was calculated as 3119,which correspond to facultative xenogamy. Reproduction biology data obtained by both methods are quite compatible with each other. Pollen viability and stigma receptivity data showed that both stigma receptivity and pollen viability were high and was no temporal isolation throughout the anthesis. Seed viability of A. argaues was determined as 52%. The low reproductive success of the species could be attributed to low seed viability and germination rates, small population size and variations in season and climate.

Effects of Mentha piperita (peppermint) extract and juice on performance and immune parameters were evaluated in laying hens. A total of 252 Babcock laying hens were allocated to seven treatments with four replications of nine hens. The control hens were fed a basal diet without supplementation. Other hens were given diets supplemented with mentha extract (ME) at 50 (50ME), 100 (100ME), and 200 (200ME) mg/kg of feed or with 50 mentha juice (50MJ), 100 (100MJ) and 200 (200MJ) mg/L that was provided in the drinking water. No significant differences were detected among treatments in bodyweight, feed intake, egg mass, egg production, eggshell breaking strength, Haugh unit, and haematological and serological parameters. The ratio of gram feed to gram egg mass (feed conversion) was significantly better in the birds that received 100ME and 200ME compared with the control hens. The yolk colour index was higher in mid trial analysis (28th day). Thus, although the ME supplementation had a positive effect on feed conversion ratio and egg yolk colour at dosage rates up to 200 mg/kg, further research is needed to establish the efficacy of this herbal product and to determine the most appropriate amount to include in diets for laying hens.

The aim of this study was to explore the potential of Pistacia terebinthus (terebinth) seed meal as a dietary supplement for laying hens. One hundred and ninety-two Babcock 30-week-old laying hens were assigned to one of six treatments (n = 32) with four replicates (n = 8). The hens were fed diets containing 0%, 1%, 2%, 3%, 4%, and 5% terebinth seed meal for eight weeks. Weekly egg production, feed consumption, egg weight, and egg mass were recorded. Egg quality was assessed at the beginning, middle and end of the study. Blood sampling was carried out on 12 birds from each treatment. Total antioxidant capacity, total oxidant status and oxidative stress index were determined. Egg production was greater from hens fed 3% and 4% terebinth than those in the other treatments. Egg weight was increased by supplementation with 2% or more terebinth. Feed consumption, feed conversion ratio, eggshell breaking strength, yolk colour, Haugh units, concentrations of glucose, total cholesterol, high density lipoprotein, low density lipoprotein, alanine aminotransferase, aspartate aminotransferase, total protein, phosphorus and calcium in serum, and total antioxidant capacity, total oxidant status, and the oxidative stress index did not differ across treatments. It is concluded that dietary terebinth seed supplementation generated positive effects on egg production and egg weight without adverse effects on egg quality or the metabolism of the hen.

Loxoscelism is caused by envenomation by spiders that belong to the Loxosceles genus. In loxoscelism, a local necrotic lesion appears and, in many cases, loxoscelism or necrotic araneism is considered a serious public health problem. There is no diagnostic test available to help the physician make a diagnostic or therapeutic decision. Here, we report the case of a severe dermonecrotic araneism (loxoscelism) in Turkey probably due to the bite of Loxosceles rufescens. There was little erythema at the beginning, followed by severe necrosis after 20 days, and skin grafting was needed although the case was treated.