Abstract The purpose of this population-based, observational, and cross-sectional study was to evaluate alterations in the oral cells of a population of older people from a Brazilian rural area, using the micronucleus technique to investigate possible associated genotoxic factors. A questionnaire was applied and clinical examination and collection of oral mucosal cells were performed for all older people (≥ 60 years) from a town in southern Brazil. Demographic and socioeconomic variables, deleterious habits (drinking and tobacco use), presence of gastro-oesophageal reflux disease (GERD), and the use of proton pump inhibitors (PPIs) were considered the exposure variables, whereas metanuclear changes (MCs) and the prevalence of cell micronuclei (MN) were considered outcomes. Out of 489 older people, 447 were included in the study, among whom 50.8% were men with a mean age of 70.9 years and 83.9% had a monthly family income greater than US$ 500.00. GERD symptoms were present in 36.2% of the individuals, and 29.1% used PPIs daily, 53.3% consumed alcoholic beverages, and 46.7% used tobacco. The analysis of 1,000 oral mucosal cells per subject showed a MN frequency of 0–2 per individual, and MCs were detected with an average of 15 units per individual (median = 11 per individual). Poisson regression did not show statistical association between the exposure variables and the outcomes (presence of MN and MCs), except for the use of PPIs, which was a protective factor for the prevalence of MN [PR 0.6 (CI 0.3–0,9)]. Age, sex, family income, tobacco use and drinking, and GERD were not associated with the number of MN and MCs in oral mucosal cells of the investigated older people.

Resumo Este estudo avaliou a carga de fratura (Cf) e o modo de falha de estruturas cerâmicas fabricadas pela técnica CAD-on (Ivoclar Vivadent), testando as hipóteses de que a Cf das estruturas multicamadas é governada pela resistência da cerâmica de cobertura e o lascamento é mais frequente nas estruturas em multicamadas do que em monolíticas. Corpos de prova no formato de disco formam fabricados da seguinte forma: CAD-on- estrutura de 3 camadas composta por infraestrutura de Y-TZP (zircônia tetragonal parcialmente estabilizada por ítria - IPS e.max ZirCAD), vidro de fusão (IPS e.max CAD Crystall/Connect) e cobertura de cerâmica vítrea a base de dissilicato de lítio (IPS e.max CAD); YLD- estrutura de 2 camadas composta por infraestrutura de Y-TZP e cobertura de cerâmica (IPS e.max Ceram); LDC- monolítico em cerâmica vítrea a base de dissilicato de lítio (IPS e.max CAD); e YZW- monolítico em Y-TZP (Zenostar Zr Translucent). Os corpos de prova foram testados sob carga de compressão até a falha e as fraturas avaliadas seguindo os princípios da fractografia. Os valores de Lf foram analisados estatisticamente usando as estatísticas de Weibull, Kruskal-Wallis e Dunn (a = 0,05). YZW (1329 N) e CAD-on (1085 N) apresentaram os maiores valores medianos de Cf, seguidos por YLD (832 N) e LDC (421 N). Todas as estruturas monolíticas (LDC e YZW) fraturaram catastroficamente e todas as estruturas YLD falharam por lascamento. A técnica CAD-on parece ser um processo de fabricação bastante promissor pois mostrou alta Cf, similar a zircônia monolítica, e baixa taxa de lascamento.

Abstract This study evaluated the fracture load (Lf) and the failure mode of CAD-on (Ivoclar Vivadent) ceramic structures, testing the hypotheses that Lf of multilayer structures is governed by the veneering ceramic strength and that chipping is more frequent in multilayer than monolithic structures. Disc-shaped specimens were fabricated as follows: CAD-on- trilayer structure composed of Y-TZP (yttria stabilized tetragonal zirconia polycrystal- IPS e.max ZirCAD) infrastructure, fusion glass-ceramic (IPS e.max CAD Crystall/Connect) and lithium disilicate-based glass-ceramic (IPS e.max CAD); YLD- bilayer structure composed of Y-TZP infrastructure and fluorapatite layering ceramic (IPS e.max Ceram); LDC- monolithic lithium-disilicate glass-ceramic (IPS e.max CAD); and YZW- monolithic Y-TZP (Zenostar Zr Translucent). The specimens were loaded in compression until failure and fracture surfaces were evaluated using fractographic principles. Lf values were statistically analyzed using the Weibull statistics, Kruskal-Wallis and Dunn tests (a= 0.05). YZW (1329 N) and CAD-on (1085 N) showed the greatest Lf median values, followed by YLD (832 N) and LDC (421 N). All monolithic structures (LDC and YZW) fractured catastrophically and all YLD structures failed by chipping. The CAD-on technique seems to be a very promising fabrication process because it showed high Lf, similar to monolithic zirconia, and small chipping rate.

RESUMO Objetivo: Avaliar a atuação odontológica em unidades de terapia intensiva. Métodos: Estudo observacional de enquete, por meio do envio de questionários via plataforma on-line de colaboração de pesquisa em terapia intensiva no Brasil (AMIBnet). A pesquisa foi realizada de junho a outubro de 2017. Os questionários, envolvendo 26 questões fechadas sobre os hospitais e a atuação odontológica nas unidades de terapia intensiva foram enviados para 4.569 profissionais de diversas especialidades atuantes nas unidades. Resultados: Obtivemos 203 questionários respondidos, resultando em uma taxa de resposta de 4,44%. A maior parte das respostas teve origem em unidades de terapia intensiva na Região Sudeste do país (46,8%). Hospitais públicos (37,9%) e privados (36,4%) tiveram participação semelhante nos resultados. Dos respondentes, 55% apontaram que um serviço de Odontologia à beira de leito estava presente, sendo prestado de maneiras diversas. Conclusão: Presença de Serviço de Odontologia e de treinamentos e protocolos de prestação de serviço em saúde bucal estiveram correlacionados. Os métodos de cuidados orais variaram sobremaneira entre as em unidades de terapia intensiva pesquisadas.

ABSTRACT Objective: To evaluate the practice of dentistry in intensive care units. Methods: An observational survey study was conducted in which questionnaires were sent via the online platform for collaboration in intensive care research in Brazil (AMIBnet). The study was carried out from June to October 2017. The questionnaires, which contained 26 closed questions about hospitals and dentistry practices in the intensive care units, were sent to 4,569 professionals from different specialties practicing in the units. Results: In total, 203 questionnaires were returned, resulting in a response rate of 4.44%. Most of the responses were from intensive care units in the Southeast region of the country (46.8%). Public hospitals (37.9%) and private hospitals (36.4%) had similar participation rates. Of the respondents, 55% indicated that a bedside dentistry service was present, and they were provided in different ways. Conclusion: The presence of dentistry services and oral health service delivery training and protocols were correlated. The oral care methods varied greatly among the intensive care units surveyed.

Resumo Os cuidados com a saúde bucal são de fundamental importância para a preservação da integridade do indivíduo e, consequentemente, refletem na saúde geral. Este estudo transversal, observacional e analítico avaliou a situação bucal de 129 alunos com deficiência intelectual matriculados na Associação de Pais e Amigos dos Excepcionais (APAE) de três municípios do sul do Brasil. Cárie dentária (índices CPO-D e ceo-d) e doença periodontal (índice PSR) foram avaliados considerando o nível de deficiência intelectual. Um questionário das condições socioeconômicas (renda e escolaridade) e última visita ao dentista foi respondida pelos pais/responsáveis. Os dados foram avaliados estatisticamente utilizando análise de variância (ANOVA) e teste Tukey (α=0,05). As médias no índice CPO-D foram de 2,27, 3,76 e 0,58 (p<0,05) e no índice ceo-d foram de 1,48, 1,55 e 2,75 para os alunos com deficiência leve, moderada e severa, respectivamente. Com relação ao índice PSR, 43% dos alunos apresentou gengivite sem fator de retenção (sem cálculo ou defeitos marginais), não tendo diferença estatística entre os três níveis de deficiência. Considerando a população e as limitações do estudo, observou-se que alunos com deficiência severa apresentaram índice CPO-D significativamente menor comparado com outros níveis de deficiência, provavelmente devido à higiene oral ser realizada pelos cuidadores/responsáveis.

Abstract Oral health care is fundamental to preserve the individual integrity and consequently influences the general health. This observational, cross-sectional and analytical study evaluated the oral condition of 129 intellectually disabled individuals from the Association of Parents and Friends of Exceptional Children (APAE) in three southern Brazilian cities. Dental caries (DMFT and dmft indices) and periodontal disease (PSR index) were evaluated considering the intellectual disability level. A questionnaire on socioeconomic status (income and education level) and the last visit to a dentist was answered by the subjects' parents/guardians. The data were statistically evaluated using analysis of variance (ANOVA) and Tukey test (α=0.05). The mean DMFT values were 2.27, 3.76 and 0.58 (p<0.05), and the mean dmft values were 1.48, 1.55 and 2.75, respectively for subjects with mild, moderate and severe disabilities. Regarding the PSR index, 43% of the subjects presented gingivitis without retention factor (no calculus or defective margins) with no significant differences among the three disability levels. Considering the population and the limitations of this study, the subjects presenting severe disabilities showed significantly lower mean DMFT values compared to other disability levels, probably because the caretakers are responsible for the oral hygiene of such subjects.

Risk factors associated with peri-implantitis are related to the biofilm composition around the implant and the ability of bacterial adhesion. This study evaluated the biofilm formation on different surfaces of commercially pure titanium (CP Ti) grade 4 after 12, 24, 48 and 168 hours using the microcosm technique and scanning electron microscopy (SEM). The following surface conditions were examined: S (control)- smooth and plain; B- sand-blasted with aluminum oxide; E- etched using nitric acid; and BE- the combination of J and A treatments. Statistical differences on biofilm formation (CFU/ mg) were found between the control (S) and B surfaces at the first 12 hours, which are related to the lowest (Ra = 0.21 µm) and highest (Ra = 0.62 µm) mean roughness values. At 168-hour all surfaces showed similar biofilm formation. Yet, microbial growth occurred on all surfaces, regardless of the surface treatment.

Este estudo realizou uma avaliação histológica do efeito das soluções irrigadoras Sterilox 400 ppm e hipoclorito de sódio (NaOCl) a 2% e 5%, com e sem a terapia fotodinâmica (PAD), na endodontia em sessão única de dentes de cães associados a lesões periapicais (APs). Dez cães foram divididos de forma randomizada em dois grupos (n=5): com e sem PAD, e os canais radiculares em quatro subgrupos, de acordo com a solução irrigadora utilizada: SX (Sterilox 400 ppm), SH2 (NaOCl 2%), SH5 (NaOCl 5%) e SS (solução salina) como controle positivo. Cento e trinta e quatro canais foram abertos e deixados expostos ao meio oral por 14 dias e depois selados por 60 dias para a indução de APs. Na sequência, os canais foram tratados de acordo com cada protocolo de desinfecção proposto e obturados na mesma sessão. Após 120 dias, os cães foram mortos e os eventos inflamatórios periapicais foram avaliados em microscopia óptica. Os dados qualitativos foram analisados estatisticamente pelos testes Kruskal-Wallis e Mann-Whitney (α=0,05). A PAD, quando empregada após o preparo químico-mecânico, não promoveu diferenças significativas nos escores para inflamação apical (p>0,05). As soluções irrigadoras SX, SH2 e SH5 sem PAD apresentaram diferenças estatísticas do grupo SS (p<0,05) que apresentou os maiores escores para inflamação apical. A PAD não apresentou efeito adicional na endodontia em sessão única dos canais com necrose pulpar associados a lesão apical e a solução irrigadora Sterilox 400 ppm pode ser considerada uma alternativa ao NaOCl na terapia endodôntica.

This study aimed to evaluate histologically the effect of irrigation with 400 ppm Sterilox, 2% and 5% sodium hypochlorite (NaOCl), with and without photo-activated therapy (PAD), in a single-visit root canal treatment of dog's teeth with apical periodontitis (AP). Ten dogs were randomly divided into two groups (n=5): with and without PAD, and the root canals into four subgroups, according to the irrigating solution: SX (400 ppm Sterilox), SH2 (2% NaOCl), SH5 (5% NaOCl) and SS (saline solution) as positive control. A total of 134 root canals were opened and left exposed to the oral environment for 14 days and then sealed for 60 days for AP induction. Then, root canals were treated according to each proposed disinfecting protocol and filled in the same session. After 120 days, the dogs were euthanized and the periapical inflammatory events were evaluated under light microscopy. Qualitative data were submitted to Kruskal-Wallis and Mann-Whitney tests (α=0.05). PAD did not produce significant differences in the scores for apical inflammation when used after chemo-mechanical preparation (p>0.05). The irrigating solutions SX, SH2 and SH5 without PAD were statistically different from SS (p<0.05) that presented the greatest scores for apical inflammation. PAD did not show any additional effect for the treatment of root canals with pulp necrosis and AP in a single visit and 400 ppm Sterilox may be considered an alternative to NaOCl in root canal treatment.

O objetivo deste estudo foi avaliar, in vitro, a liberação de flúor (F), ao longo do tempo, de três sistemas adesivos: Clearfil Protect Bond (CPB-Kuraray), FL Bond II (FLB-Shofu), Adper Single Bond 2 (SB2-3M ESPE) (controle negativo). Os sistemas adesivos CPB e FLB contêm flúor em sua composição e liberam F por diferentes mecanismos. A hipótese testada foi a de que o mecanismo de liberação de F influencia a quantidade de F liberado em água. Foram confeccionados espécimes em forma de discos (5 mm × 3 mm) utilizando uma matriz de plástico (Demetron Research Corp). Foram produzidas 3 amostras para cada um dos materiais, para cada período de avaliação (n=3) (1, 7, 14, 21 e 28 dias). Subsequentemente, as amostras foram armazenadas em 10 mL de água destilada a 37 °C até o momento das leituras que foram feitas usando uma membrana líquida para eletrodo seletivo de íons F (Orion 710). Foram realizadas quatro leituras no primeiro dia; após foi feita uma leitura/dia. Os resultados foram analisados estatisticamente por ANOVA e teste de Tukey (α=0,05). O sistema adesivo CPB liberou a maior quantidade de flúor em todos os períodos avaliados, com o maior valor em 6 H (0,183 ppm), então ela diminuiu gradualmente até o sétimo dia, quando aumentou significativamente até o dia 21. Na maioria das análises realizadas, FLB mostrou valores de liberação de flúor semelhantes aos de SB2. Portanto, o mecanismo de liberação de flúor influenciou a quantidade de fluoreto de liberado em água, confirmando a hipótese do estudo.

The objective of this study was to evaluate in vitro the time-dependent fluoride (F) release from three adhesive systems: Clearfil Protect Bond (CPB - Kuraray), FL Bond II (FLB- Shofu) and Adper Single Bond 2 (SB2 - 3M ESPE) (negative control). CPB and FLB are fluoride containing adhesives that use different F releasing mechanisms. The tested hypothesis was that the F releasing mechanism influences the amount of released F in water. Disc-shaped specimens (5 mm × 3 mm) were fabricated using a plastic matrix (Demetron Research Corp). Three specimens were produced for each material and each period of evaluation (1, 7, 14, 21 and 28 days) (n=3). Subsequently, the specimens were stored in 10 mL distilled water at 37° C until the analyses were done using a liquid membrane for selective F ion electrode (Orion 710). Four readings were performed on the first day and the remaining evaluation times had one reading/day. Results were statistically analyzed by two-way ANOVA and Tukey's test (α=0.05). CPB released the greatest amount of fluoride in all evaluated periods with the greatest value at 6th h (0.183 ppm) thereafter decreasing gradually up to the 7th day when it significantly increased again until the 21st day. In most measurements, FLB showed similar mean fluoride release values as SB2. Therefore, the fluoride release mechanism influenced the amount of fluoride released in water, confirming the study hypothesis.

The objective of the study was to measure the marginal and internal fit of zirconia-based all-ceramic three-unit fixed partial dentures (FPDs) (Y-TZP - LAVA, 3M-ESPE), using a novel methodology based on micro-computed tomography (micro-CT) technology. Stainless steel models of prepared abutments were fabricated to design FPDs. Ten frameworks were produced with 9 mm2 connector cross-sections using a LAVATM CAD-CAM system. All FPDs were veneered with a compatible porcelain. Each FPD was seated on the original model and scanned using micro-CT. Files were processed using NRecon and CTAn software. Adobe Photoshop and Image J software were used to analyze the cross-sectional images. Five measuring points were selected, as follows: MG - marginal gap; CA - chamfer area; AW - axial wall; AOT - axio-occlusal transition area; OA - occlusal area. Results were statistically analyzed by Kruskall-Wallis and Tukey's post hoc test (α= 0.05). There were significant differences for the gap width between the measurement points evaluated. MG showed the smallest median gap width (42 µm). OA had the highest median gap dimension (125 µm), followed by the AOT point (105 µm). CA and AW gap width values were statistically similar, 66 and 65 µm respectively. Thus, it was possible to conclude that different levels of adaptation were observed within the FPD, at the different measuring points. In addition, the micro-CT technology seems to be a reliable tool to evaluate the fit of dental restorations.

O objetivo deste estudo foi avaliar a fenda marginal vertical de copings de ouro sinterizado e coroas metalo-cerâmicas com diferentes tipos de término de preparo: ombro arredondado biselado (OAB) e chanfro longo biselado (CLB), testando a hipótese nula de que não há diferenças na adaptação marginal quando se compara o tipo de término ou a fase da restauração (coping ou coroa). Modelos de aço inoxidável foram confeccionados simulando preparos dentais com diferentes términos (OAB e CLB). Dez modelos de gesso foram confeccionados a partir de cada preparo. Duas camadas de espaçador foram passadas em cada preparo, a 1 mm da margem. Os copings de Sintercast confeccionados, sinterizados e ajustados ao modelo de gesso. Os copings (n=10) foram colocados no modelo de aço e a fenda marginal foi medida em 24 pontos equidistantes por meio de microscópio óptico (×230). Uma camada de opaco e duas de porcelana feldspática foram aplicadas sobre os copings e o mesmo processo de mensuração de fenda foi utilizado para as coroas. Os dados foram analisados estatisticamente usando o teste t Student pareado e não-pareado (α=0,05). Os valores médios de fenda marginal (µm) para os copings e coroas foram, respectivamente: 113,6 e 117,1 para OAB; e 58,2 e 74,3 para CLB. Fendas marginais significantemente maiores (p=0,0307) foram observadas nas restaurações com términos OAB quando comparados ao término CLB, o qual mostrou diferenças estatísticas na comparação entre coping e coroa (p=0,001). Em conclusão, a fenda marginal é influenciada pela aplicação de cerâmica em copings fabricados com términos CLB, e fendas marginais maiores foram observadas para restaurações com término OAB, rejeitando-se as hipóteses nulas.

The aim of this study was to evaluate the vertical marginal gap of sintered gold copings and metal-ceramic crowns with different finish line preparations: a beveled round shoulder (BRS) and a beveled long chamfer (BLC), testing the null hypotheses that there are no differences in marginal gap regardless of finish line and phase of restoration (coping or crown). Stainless steel master models were fabricated to simulate tooth preparation for metal-ceramic crowns with different finish lines (BRS and BLC). Ten dies were obtained from each model. Preparations were coated with 2 layers of spacer to 1 mm from the margin. Sintercast gold copings were prepared, sintered and adjusted to the dies. The copings (n=10) were placed onto the master model and the marginal gap was measured in 24 equidistant points using optical microscopy (X230). An opaque and two body ceramic layers were subsequently applied to the copings and the same measuring procedure was performed for the crowns. The data were analyzed statistically using paired and unpaired Student's t-test (α=0.05). Mean marginal gap values (µm) for the copings and crowns were, respectively: 113.6 and 117.1 for the BRS; and 58.2 and 74.3 for the BLC preparation. Significantly greater marginal gaps (p=0.0307) were found for restorations with BRS than with BLC finish line, which also showed statistically significant differences in the gap size between coping and crown (p=0.001). In conclusion, marginal gap is influenced by ceramic application on copings fabricated on BLC preparation, and greater marginal gaps were found for restorations with BRS finish line, rejecting the experimental null hypotheses.

Este estudo avaliou o uso de normas científicas em artigos de Odontologia publicados em periódicos brasileiros e internacionais. Trabalhos publicados de janeiro de 2006 a dezembro de 2010 foram examinados nas seguintes revistas: Brazilian Dental Journal (BDJ), Brazilian Oral Research (BOR); Journal of Applied Oral Science (JAOS), Journal of Dental Research (JDR) e Dental Materials (DM). Além do título, tipo de estudo, volume e ano de publicação, as seguintes informações foram registradas a partir de cada artigo: identificação de normas relatadas no estudo; em caso negativo, relatar se alguma norma poderia ser aplicada no estudo. Um total de 3.046 artigos foi examinado como segue: 937 no DM, 936 no JDR, 489 no JAOS, 348 BDJ e 336 no BOR. Considerando os artigos que poderiam usar alguma norma, o DM apresentou o maior percentual (24,9%) de normas/artigos relatados, seguido pelo JAOS (10,3%), BOR (10,1%), BDJ (6,3%) e JDR (2,4%), o que significa que de 936 trabalhos analisados do JDR, 623 poderiam ter usado alguma norma, mas apenas 15 relataram o uso. De todos os trabalhos examinados, 309 (10,1%) estudos relataram alguma norma. As normas ISO foram relatadas na maior parte (57,8%), seguido pelas normas ASTM (23,7%) e 74 (18,5%) feitas por outras organizações internacionais (por exemplo, ADA e CIE). Apesar do alto fator de impacto dos periódicos selecionados, seus artigos publicados poderiam utilizar as normas com maior frequência. Este estudo deve alertar os editores de periódicos a incentivar os autores a consultar as normas disponíveis para apoiar a metodologia dos trabalhos científicos.

This study evaluated the use de standards in papers published in Brazilian and international dental journals. Papers published from January 2006 to December 2010 in the following journals were examined: Brazilian Dental Journal (BDJ), Brazilian Oral Research (BOR), Journal of Applied Oral Sciences (JAOS), Journal of Dental Research (JDR) and Dental Materials (DM). In addition to the title and type of study, issue, volume and year of publication, the following information was recorded from each paper: identification of any standard reported in the study; in case of no reported standard, indication if a standard could be used in the study. A total of 3,046 papers were examined, being 937 from DM, 936 from JDR, 489 from JAOS, 348 from BDJ, and 336 from BOR. Considering the papers that could use some standard, DM showed the highest percentage (24.9%) of reporting standards/paper, followed by JAOS (10.3%), BOR (10.1%), BDJ (6.3%) and JDR (2.4%), meaning for example that, from 936 papers examined from the JDR, 623 could use some standard but only 15 papers reported them. From all papers examined, 309 (10.1%) reported using some standard. The ISO standards were mostly reported (57.8%), followed by ASTM standards (23.7%) and 74 (18.5%) standards from other international organizations (e.g. ADA and CIE). Despite of the high impact factors of the selected dental journals, their published papers could use standards more often. This study should assist journal editors to encourage authors to consult and refer to available standards to support the scientific papers.

OBJECTIVES: This in vitro study evaluated the effectiveness of three carbamide peroxide concentrations used for tooth bleaching treatments. MATERIAL AND METHODS: Sixty bovine dental slabs (6x6x3 mm) were obtained, sequentially polished, submitted to artificial staining (baseline) and randomized into four groups (n=15), according to the bleaching agent concentration: distilled water (control), 10% (CP10), 16% (CP16) or 37% (CP37) carbamide peroxide. CP10 and CP16 were covered with 0.2 mL of the respective bleaching gels, which were applied on enamel surface for 4 h/day during two weeks. Samples of CP37 were covered with 0.2 mL of the bleaching gel for 20 min. The gel was light activated by two 40-s applications spaced by 10-min intervals. The gel was renewed and applied 3 times per clinical session. This cycle was repeated at 3 sessions with 5 days of interval between them. Tooth shade evaluations were done with a digital spectrophotometer at T0 (baseline), T1 (after 1-week of treatment) and T2 (1-week post-bleaching). Tooth shade means were statistically analyzed by Kruskal-Wallis and Friedman's tests and color parameters were analyzed by two-way ANOVA and Tukey's test (p<0.05). RESULTS: At T1 and T2 evaluations, tooth shade was significantly lighter than at baseline for all treatment groups, considering the color parameters ΔL*, Δa*, Δb*, ΔE* (p<0.001) or tooth shade means (p<0.001). CP37 group showed lower shade mean change than CP10 and CP16 at T1 (p<0.01), but this difference was not statistically significant at T2 (p>0.05). CONCLUSIONS: One week after the end of the treatment, all carbamide peroxide concentrations tested produced similar tooth color improvement.

Intracanal medication in pulpectomy therapy is used between appointments with the objective of reducing pain and inflammatory processes in pulp and periapical tissues. Propolis has been known as a natural antibiotic and has been subject of medical and dental research due to its therapeutic properties such as antibiotic, analgesic and anti-inflammatory effects. OBJECTIVE: The aim was to carry out an in vivo evaluation of the periapical tissue response to propolis paste when used as an intracanal medication in the teeth of dogs after pulpectomy. MATERIAL AND METHODS: 72 dog's incisors were selected for the experiment. After biomechanical preparation the root canal was filled with a corticosteroid-antibiotic preparation, experimental propolis paste, non-medicament (negative control) or non-pulpectomy at all (positive control). The medications were left inside the root canal for 7, 14 or 28 days. At the end of the experimental period histological sections were prepared and all laboratories processes for Harris hematoxylin and eosin staining was proceeded followed by the analysis using an optical microscope. Sections were classified according to a score representing the inflammatory events observed: the presence of polymorphonuclear neutrophils, polymorphonuclear eosinophils, lymphocytes and plasma cells, macrophages and/or giant cells, fibrous condensation and abscesses. RESULTS: There were statistically significant differences between the tissue reactions caused by the two substances being tested, after different experimental periods, with the periapical tissue that was in contact with propolis paste exhibiting fewer inflammatory reactions in comparison to corticosteroid-antibiotic preparation. CONCLUSIONS: The low tissue responses from propolis paste suggest that this material could be considered as an option for root canal medication after pulpectomy.

O objetivo deste estudo foi avaliar, in vitro, a liberação de flúor (F) de 4 materiais restauradores (3M-ESPE): KME cimento de ionômero de vidro convencional (CIV) (Ketak Molar Easymix); RX2- GIC modificado por resina (RMGIC) (Rely-X luting 2); VIT-RMGIC (Vitremer); -resina composta Z250 (controle negativo) (Filtek Z250). As amostras foram confeccionadas de acordo com as instruções do fabricante e colocadas em 10 mL de água destilada 37°C até as leituras, que foram feitas utilizando um eletrodo de íon seletivo de fluoreto de membrana líquida (Orion 710). A liberação de flúor foi avaliada a cada 6 h no primeiro dia e, diariamente durante 28 dias (d). Os resultados foram analisados estatisticamente usando ANOVA a dois critérios e teste de Tukey. A média e desvio padrão de liberação de F, valores (em ppm) foram: KME: 6 h- 0,34 ± 0,04; 24 h- 1,22 ± 0,30; 7 d- 0,29 ± 0,09; 14 d- 0,20 ± 0,04; 28 d- 0,16 ± 0,01; RL2: 6 h- 2,46 ± 0,48; 24 h- 12,33 ± 2,93; 7 d- 1,37 ± 0,38; 14 d- 0,80 ± 0,13; 28 d- 0,80 ± 0,21; VIT: 6 h- 0,98 ± 0,35; 24 h- 4,35 ± 1,22; 7 d- 0,66 ± 0,23; 14 d- 0,40 ± 0,07; 28 d- 0,39 ± 0,08; Z250: 6 h- 0,029 ± 0,001; 24 h- 0,024 ± 0,009; 7 d- 0,023 ± 0,004; 14 d- 0,025 ± 0,001; 28 d- 0,028 ± 0,001. Concluiu-se que RL2 liberou mais F do que os outros materiais em todos os períodos. A maior liberação de F ocorreu nas primeiras 24 h.

The aim of this study was to evaluate in vitro fluoride (F) release from 4 restorative materials (3M ESPE): Ketak Molar Easymix [KME - conventional glass ionomer cement (GIC)]; Rely-X luting 2 [RL2 - resin-modified GIC (RMGIC)]; Vitremer (VIT- RMGIC); and Filtek Z250 [Z250 - negative control]. Disc-shaped specimens were fabricated according to the manufacturer’s instructions and placed into 10 mL of reverse osmosis water at 37°C until the analyses were done using a liquid membrane for selective F ion electrode (Orion 710). F release was evaluated every 6 h in the first day and thereafter daily during 28 days (d). The results were analyzed statistically by two-way ANOVA and Tukey’s test (α=0.05). Mean F release and standard deviation values (in ppm) were: KME: 6 h- 0.34 ± 0.04; 24 h- 1.22 ± 0.30; 7 d- 0.29 ± 0.09; 14 d- 0.20 ± 0.04; 28 d- 0.16 ± 0.01; RL2: 6 h- 2.46 ± 0.48; 24 h-12.33 ± 2.93; 7 d- 1.37 ± 0.38; 14 d- 0.80 ± 0.13; 28 d- 0.80 ± 0.21; VIT: 6 h- 0.98 ± 0.35; 24 h- 4.35 ± 1.22; 7 d- 0.66 ± 0.23; 14 d- 0.40 ± 0.07; 28 d- 0.39 ± 0.08; Z250: 6 h- 0.029 ± 0.001; 24 h- 0.024 ± 0.009; 7 d- 0.023 ± 0.004; 14 d- 0.025 ± 0.001; 28 d- 0.028 ± 0.001. RL2 RMGIC released more F than the other materials in all periods. The greatest release of F occurred in the first 24 h.

O objetivo deste estudo foi avaliar o efeito de diferentes tempos de condicionamento ácido na rugosidade de superfície e resistência flexural de uma cerâmica à base de disilicato de lítio. Espécimes cerâmicos em forma de barra (16 mm x 2 mm x 2 mm) foram produzidos a partir de blocos cerâmicos. Todos os espécimes foram polidos e limpos em banho de ultrasom em água destilada. Os espécimes foram aleatoriamente divididos em 5 grupos (n=15). Grupo A (controle) sem tratamento. Grupos B-E condicionamento com ácido fluorídrico 4,9% (HF) por 4 diferentes períodos de condicionamento: 20 s, 60 s, 90 s e 180 s, respectivamente. As superfícies condicionadas foram observadas sob microscopia eletrônica de varredura. Perfilometria de superfície foi utilizada para examinar a rugosidade das superfícies condicionadas, e os espécimes foram carregados até a falha pelo teste de flexão três pontos. Os valores foram analisados usando ANOVA um fator e teste de Tukey (?=0,05). Todos os períodos de condicionamento produziram superfícies significantemente mais rugosas do que o grupo controle (p<0,05). Os valores de rugosidade aumentaram com o tempo de condicionamento. Os valores médios de resistência à flexão foram (MPa): A=417 ± 55; B=367 ± 68; C=363 ± 84; D=329 ± 70; e E=314 ± 62. O condicionamento com HF reduziu significativamente os valores médios de resistência à flexão conforme o tempo de condicionamento aumentou (p=0,003). Os achados deste estudo mostraram que o aumento do tempo de condicionamento ácido influenciou a rugosidade de superfície e resistência flexural de uma cerâmica à base de disilicato de lítio confirmando a hipótese do mesmo.

The aim of this study was to examine the effect of different acid etching times on the surface roughness and flexural strength of a lithium disilicate-based glass ceramic. Ceramic bar-shaped specimens (16 mm x 2 mm x 2 mm) were produced from ceramic blocks. All specimens were polished and sonically cleaned in distilled water. Specimens were randomly divided into 5 groups (n=15). Group A (control) no treatment. Groups B-E were etched with 4.9% hydrofluoric acid (HF) for 4 different etching periods: 20 s, 60 s, 90 s and 180 s, respectively. Etched surfaces were observed under scanning electron microscopy. Surface profilometry was used to examine the roughness of the etched ceramic surfaces, and the specimens were loaded to failure using a 3-point bending test to determine the flexural strength. Data were analyzed using one-way ANOVA and Tukey's test (?=0.05). All etching periods produced significantly rougher surfaces than the control group (p<0.05). Roughness values increased with the increase of the etching time. The mean flexural strength values were (MPa): A=417 ± 55; B=367 ± 68; C=363 ± 84; D=329 ± 70; and E=314 ± 62. HF etching significantly reduced the mean flexural strength as the etching time increased (p=0.003). In conclusion, the findings of this study showed that the increase of HF etching time affected the surface roughness and the flexural strength of a lithium disilicate-based glass ceramic, confirming the study hypothesis.