ABSTRACT Successive applications of swine manure can promote changes in soil physical attributes, with emphasis on the soil aggregation pathways. The objective of this work was to separate and quantify the aggregates according to their training path and to evaluate the stability of these aggregates in an area with a history of applications of pig slurry (PS) and deep litter (DL) in no-tillage system (NTS). After 10 years of use swine manure (SM) in oat/corn succession under NTS, undisturbed soil samples were collected in the 0-5 and 5-10 cm layers, in the treatments without SM (control), with application of PS and DL at doses equivalent to one and two times the recommended N for corn and oats (PS1X, PS2X, DL1X and DL2X, respectively). The aggregates were separated according to their morphological patterns and biogenic and physicogenic formation pathways. Then, weighted average diameter (WMD) of the aggregates and the distribution of the aggregates in the diameter classes for macroaggregates (8.0>Ø ≥2.0 mm), mesoaggregates (2.0>Ø ≥0.25 mm) and microaggregates (Ø<0.25 mm) in each type of aggregate were evaluated. The addition of SM increased the formation of biogenic aggregates. In the control area, the formation pathway of physicogenic aggregates prevails. The application of PS did not alter the WMD and the macroaggregates in relation to the control, while the application of DL increased these parameters when compared to the PS and control treatments.

RESUMO Aplicações sucessivas de dejetos suínos podem promover mudanças nos atributos físicos do solo, com ênfase nas vias de formação dos agregados solo. O objetivo deste trabalho foi separar e quantificar os agregados conforme sua via de formação e avaliar a estabilidade desses agregados em área com histórico de aplicações de dejeto líquido de suínos (DLS) e cama sobreposta de suínos (CSS), em sistema plantio direto (SPD). Após 10 anos do uso de dejetos suínos (DS) na sucessão aveia/milho sob SPD, coletaram-se amostras indeformadas de solo nas camadas de 0-5 e 5-10 cm, nos tratamentos sem DS (testemunha), com aplicação de DLS e CSS em doses equivalentes a uma e duas vezes a recomendação de N para o milho e a aveia (DLS1X, DLS2X, CSS1X e CSS2X, respectivamente). Os agregados foram separados conforme os seus padrões morfológicos e vias de formação em biogênicos e fisiogênicos. Em seguida, avaliaram-se o diâmetro médio ponderado (DMP) dos agregados e a distribuição dos agregados nas classes de diâmetro para macroagregados (8,00 > Ø ≥ 2,0mm), mesoagregados (2,0 > Ø ≥ 0,25mm) e microagregados (Ø < 0,25mm), em cada tipo de agregado. A adição de dejetos suínos aumentou a formação de agregados biogênicos. Na área testemunha prevalece a via de formação de agregados fisiogênicos. A aplicação de DLS não alterou o DMP e os macroagregados em relação à testemunha, enquanto a aplicação de CSS aumentou esses parâmetros quando comparada aos tratamentos DLS e Testemunha.

ABSTRACT Crotalaria juncea is used as plant cover in grape vineyards in Brazil, which usually present soils with high copper (Cu) levels due to the application of Cu-based phyto-sanitary products. Under this condition an increase growth and cover of C. juncea is needed to improve the phytoremediation processes in those soils. Some alternatives to achieve this condition is the inoculation with arbuscular mycorrhizal fungi (AMF), which has demonstrated an important increase of plant growth in Cu-contaminated soils at different soil P levels. The aims of this study were to evaluate the effect of AMF inoculation in soils with high Cu contents on the growth of C. juncea, the acid phosphatase (APase) enzyme activity in plants and soil, and the presence of glomalin under different P supply conditions, as a basis to identify if there is a synergistic interaction between AMF inoculation and P supply on soils with high Cu levels. The experiment was carried under greenhouse conditions in a factorial 3 × 2 design (natural P content, addition of 40 and 100 mg kg-1 P, with and without the inoculation of the AMF Rhizophagus clarus with three replicates) in a soil with high Cu content (60 mg kg-1). The addition of 40 and 100 mg kg-1 P favored plant growth both in the presence and in the absence of AMF. However, when plants were grown in soil with a natural P level, the inoculation with AMF increased by 116 % the shoot biomass, compared to the non-inoculated treatment. Our results showed that the combination of P supply and R. clarus inoculation could be an adequate strategy to reduce Cu phytotoxicity in C. juncea, as it increases plant biomass and modify the APase enzyme activity in the soil and plant. Additionally, glomalin produced by the AMF and accumulated in the soil can decrease the availability of Cu to the plants by means of sequestration beyond the root surface, with a consequent plant protective effect.

Abstract The aim of this research was to evaluate the physical attributes of Cambisol in apple (Malus domestica Borkh. cv. Gala) orchard under different weed management systems in the state of Santa Catarina (SC), Brazil. The treatments were as follows: control (no weed management), chemical desiccation and mowing. We took samples of undisturbed soil at depths of 0-5, 5-10, 10-15 and 15-20 cm. We evaluated hydraulic conductivity (HC); bulk density (BD); soil penetration resistance (Pr); total porosity (TP), macropores (MaP), micropores (MiP); soil aggregation indices such as arithmetic and geometric mean diameter of air-dried and water-stable aggregates (AMDad, AMDws, GMDad, GMDws, respectively); aggregate stability index (ASIAMD and ASIGMD) and volumetric water content (VWC). We also carried out principal component analysis (PCA) with the data. The mowing or desiccation treatments increased BD at the 15-20 cm depth compared to control. The mowing treatment had the highest ASIGMD while desiccation contributed to the lowest ASIGMD. The desiccation treatment decreased AMDws (0-10 cm), ASIAMD (0-5 cm) GMDws (5-10 cm) and reduced HC by 26% and 22%, respectively, compared to the mowing and control treatments. Through PCA we were able to separate the three treatments. The mowing treatment was correlated with HC, VWC at pressure of 600 kPa and ASIGMD; desiccation was correlated with BD and Pr; and control with ASIAMD, MaP and TP.

Resumo O objetivo do trabalho foi avaliar os atributos físicos de Cambissolo em pomar de macieira (Malus domestica Borkh. cv. Gala) em Santa Catarina, Brasil, submetido a diferentes manejos das plantas espontâneas. Os tratamentos avaliados foram: testemunha (sem manejo das espontâneas), dessecação química e roçada. Foram coletadas amostras indeformadas de solo nas profundidades de 0-5, 5-10, 10-15 e 15-20 cm, nas quais foram avaliadas a condutividade hidráulica (CH); densidade do solo (Ds); resistência do solo à penetração (Rp); porosidade total (PT), macroporos (MaP), microporos (MiP); índices de agregação do solo, sendo o diâmetro médio aritmético e geométrico dos agregados secos ao ar e estáveis em água (DMAsa, DMAea, DMGsa, DMGea, respectivamente); índice de estabilidade de agregados (IEADMA e IEADMG) e umidade volumétrica (UV). Também realizou-se uma análise de componentes principais (ACP) com os dados obtidos. O manejo com roçada ou dessecação aumentou a Ds (15-20 cm) quando comparado com a testemunha. O tratamento roçada foi responsável por apresentar o maior IEADMG, enquanto a dessecação contribui para o menor IEADMG. O manejo com a dessecação diminuiu o DMAea (0-10 cm), IEADMA (0-5 cm), DMGea (5-10 cm) e reduziu a CH em 26% e 22%, respectivamente, em relação à roçada e testemunha. Por meio da ACP foi possível separar os três tratamentos avaliados, sendo o manejo com a roçada correlacionado com a CH, UV na tensão de 600 kPa e IEADMG; a dessecagem correlacionado com DS e RP; e a testemunha correlacionada com IEADMA, MaP e PT.

ABSTRACT Applications for long periods of organic animal waste as a source of nutrients to crops can contaminate the soil and the surface and subsurface waters. This study aimed to evaluate how the utilization of organic waste, after successive applications, affects P fractions in the soil and consequently environmental contamination. In an experiment conducted for 8 years, totaling 12 applications with pig slurry manure (PSM), liquid cattle manure (LCM), swine deep bedding (SDB), and mineral fertilizer (NPK), 460; 505; 1.111; and 535 kg P∙ha−1 were added to the soil through PSM, LCM, SDB, and NPK, respectively. In September 2012, soil samples were collected in the layers 0.0 – 0.04; 0.04 – 0.08; 0.08 – 0.12; 0.12 – 0.16; 0.16 – 0.20 m, prepared and subjected to a sequential chemical fractionation of P. The 12 applications of pig slurry, liquid cattle manure, swine deep bedding and NPK fertilizer for eight years (urea + triple superphosphate + potassium chloride) provided accumulations of P fractions in the soil that were above the necessary for a proper nutrition to plants considering the available P fractions (AER + organic NaHCO3 + inorganic NaHCO3) in the soil. In addition, they showed that the use of swine deep bedding, followed by pig slurry, means higher levels of available P fractions in the soil. In this sense, the establishment of a dosage of organic waste to meet the nitrogen need of crops is not an environmentally appropriate parameter. It may cause P accumulation in the soil, increased absorption ability of plants and soil support, contributing to environmental contamination.

O objetivo deste estudo foi avaliar o efeito do consumo agudo de cafeína sobre a oxidação de lipídeos e desempenho durante o exercício aeróbico. Foram avaliados 15 indivíduos do sexo masculino, com idade média de 22,3 ± 2,7 anos, que realizaram teste de cargas progressivas em esteira rolante para determinação do consumo máximo de oxigênio (VO2máx) e limiares ventilatórios (LV). Cada voluntário realizou três testes submáximos na intensidade de 10% abaixo do segundo LV, sendo orientados a permanecer em exercício até a exaustão. Trinta minutos antes de cada teste submáximo, foram ingeridos 250ml de uma das bebidas compostas por: café com adoçante (CAD), café com açúcar (CA) e café descafeinado com adoçante (CD). Durante o exercício, os indivíduos foram monitorados pelo ergoespirômetro e frequencímetro. A oxidação de lipídeos foi predita pelo quociente respiratório (QR) durante o teste, e o desempenho foi verificado pelo tempo de exercício. Para comparar os resultados de QR e tempo de exercício entre os grupos, foi utilizado Anova fatorial, e considerou-se significância estatística um valor de p < 0,05. A média de VO2máx foi de 50,18 ± 9,9ml/kg/min. Com a ingestão de CAD, a média do QR foi de 0,98 ± 0,18 e o tempo médio em exercício foi de 24,1 ± 17,04 minutos. Com a ingestão de CA, a média do QR foi de 0,96 ± 0,2 e o tempo médio em exercício foi de 24,4 ± 17,8min. No teste com ingestão de CD, a média do QR foi de 1,01 ± 0,24 e a média do tempo em exercício foi de 20,6 ± 9,7min. Não houve diferença significativa entre os testes nos valores do QR e nem tempo de exercício (p = 0,697 e p = 0,598, respectivamente). A cafeína não aumentou a oxidação de lipídeos nem o desempenho de indivíduos jovens do sexo masculino.

The purpose of this study was to evaluate the effect of acute caffeine consumption on lipid oxidation and performance during aerobic exercise. Fifteen healthy male individuals, 22.3 ± 2.7 years old, performed a progressive test on treadmill for determination of maximal oxygen uptake (VO2max) and ventilatory thresholds. Each volunteer performed three submaximal tests at the intensity of 10% below the second ventilatory threshold, being guided to remain on exercise until exhaustion. Thirty minutes before each submaximal test, the subjects ingested 250ml of one of following drinks: coffee with sweetener (CSW), coffee with sugar (CS) or decaffeinated coffee with sweetener (CD). During the exercise, the individuals's heart rate was monitored and respiratory gases analyses were done. The lipid oxidation was predicted by the respiratory quotient (RQ) during the test and performance was verified by exercise duration. In order to compare the RQ results and time of exercise among the three groups, factorial Anova was used, and a value of p < 0.05 was considered as statistically significant. The individuals had VO2max of 50.18 ± 9.9 ml/kg/min. CAD ingestion caused RQ average of 0.98 ± 0.18, and the average exercise duration was of 24.1 ± 17.04 min; CA ingestion caused RQ average of 0.96 ± 0.2 and the average exercise duration was 24.4 ± 17.8 min. Finally, CD ingestion caused the RQ average of 1.01 ± 0.24, and the average exercise duration was of 20.6 ± 9.7 min. There were no significant differences in the RQ values or exercise duration among the three interventions (p = 0.697 and p = 0.598, respectively). Caffeine did not increase lipid oxidation or performance of young male individuals.

Exposure to indoor allergens is an important risk factor for sensitisation and respiratory allergy. The aim of this paper was to evaluate the levels of mite, cat and latex allergens in dust collected from an indoor workplace and to assess whether the exposure to these allergens was associated with the allergy symptoms reported by employees. Sixty dust samples were collected. Allergen concentrations were measured with antibody based ELISAs. All 144 participants compiled a questionnaire exploring possible symptoms of allergy. No association between latex allergen exposure and symptoms was found in spite of the high frequency of latex allergens. Mite allergens were detected in a minority of rooms. Cat allergen was the most important indoor allergen in the sampled workplace and exposure to this allergen could represent a risk for employees.

L'esposizione agli allergeni indoor costituisce un importante fattore di rischio per lo sviluppo di allergie. L'obiettivo di questo articolo è valutare i livelli degli allergeni degli acari, del gatto e del lattice in campioni di polvere raccolti in un ambiente lavorativo, al fine di stabilire una eventuale relazione tra l'esposizione a questi allergeni ed i sintomi di allergia manifestati dai dipendenti. La concentrazione degli allergeni è stata analizzata in sessanta campioni e 144 dipendenti hanno compilato un questionario avente lo scopo di valutare eventuali sintomi di allergia. Nonostante l'elevata frequenza degli allergeni del lattice, non esiste associazione tra sintomi ed esposizione a questi allergeni. La presenza di allergeni degli acari è stata riscontrata in una minoranza di ambienti. L'allergene del gatto è l'allergene indoor più diffuso nell'ambiente lavorativo esaminato e l'esposizione a tale allergene può costituire un rischio per i dipendenti.