In this work, adult stem cells of three volunteers were isolated, expanded and cultivated over samples of Anorthite in order to assess its osteoinductive capacity. Alkaline Phosphatase Analysis, ALP, was carried out at days 1, 7, 14 and 21, and the results showed similar behavior between the volunteers considering the initial value of each. For volunteer 1, between day 14 and 21, the decreasing of ALP was remarkable in the wells containing MSC only and MSC+BIO, from 59.9 to 26.3 U.L-1 and from 63.3 to 38.1 U.L-1, respectively, suggesting that the biomaterial was able to induce osteoblast formations. This osteoinduction property could be evidenced by Citochemistry where MSCs did not produce crystals of Calcium Oxalate or Calcium Phosphate, osteoblast compounds, without being stimulated by a chemical inducer, lending to the conclusion that the differentiation of MSC into osteoblast when cultivated on Anorthite, occurred exclusively by its influence, strongly suggesting that it is osteoinductive.

Este trabalho descreve aspectos ultraestruturais e citoquímicos de Lepista sordida. Basidiósporos recobertos com ornamentações verruculosas e irregulares foram observados através de microscopia eletrônica de varredura. Através de microscopia confocal verificou-se que a maioria apresentava um grande corpo lipídico, ausência de vesículas ácidas e núcleo localizado na periferia.

Some ultrastructural and cytochemical aspects of Lepista sordida are described. Basidiospores with verruculose and irregular ornamentations were observed through scanning electron microscopy. Confocal microscopy indicated that most of them exhibited a single large lipid body, no acidic vesicles and nucleus localized in the cell periphery.