Capillary electromigration techniques can be understood as the ones used to separate neutral compounds, solvated ions and ionized species, considering the differentiated migration among them when an electrical field is applied within a capillary column filled with a background electrolyte. Several existing capillary electrophoresis setups provide the possibility of developing reliable quantitative assays of inorganic ions, organic acids, fatty acids, amino acids, carbohydrates, nucleic acids, proteins, hormones, vitamins, macromolecules, and more. Therefore, given the chemical complexity of bodily fluid matrices, capillary electrophoresis (CE) and its variations present themselves as an advantageous strategy for enhancing traditional clinical diagnostic methods and for the development of new ones. The recent advances in the study of the human metabolome associated with technological improvements towards medical applications make CE a very useful and versatile technique for clinical laboratory assays. Within this context, this review has the purpose of presenting some traditional methods used for exams of blood, urine, saliva, feces, and sweat, and how CE can be implemented as a real alternative to enable faster, automated, and cost-effective analysis with a comprehensive perspective that shall result in better diagnostic possibilities for patients, being a powerful tool for helping physicians on the precision medicine achievements. compounds species electrolyte acids carbohydrates proteins hormones vitamins macromolecules more Therefore matrices (CE context blood urine saliva feces sweat faster automated costeffective cost effective patients achievements

Resumo Numerosos estudos têm investigado a composição química e as atividades biológicas de óleos essenciais extraídos de cascas dos frutos, folhas e flores de diferentes espécies de Citrus. Este trabalho tem como objetivo investigar a composição química e as atividades larvicida e leishmanicida in vitro do óleo essencial das cascas dos frutos de Citrus reticulata (CR-EO). CR-EO foi obtido pela técnica de extração em aparelho Clevenger e sua composição química foi determinada por CG-EM e CG-DIC. Limoneno (85,7%), ɣ-terpineno (6,7%) and mirceno (2,1%) foram identificados como os constituintes majoritários. CR-EO mostrou alta atividade contra as formas promastigota de Leishmania amazonensis (CI50 = 8,23 µg/mL). CR-EO também exibiu alta atividade larvicida contra as larvas do terceiro estágio do Aedes aegypti com concentração letal (CL50 = 58,35 µg/mL) e mortalidade de 100% em 150 µg/mL. Este estudo sugere, pela primeira vez, o uso potencial de CR-EO contra esta importante doença viral transmitida por mosquitos do gênero Aedes.

Abstract Numerous studies have investigated the chemical composition and biological activities of essential oils from different Citrus species fruit peel, leaves and flowers. This paper aims to investigate the chemical composition, larvicidal and antileishmanial activities of essential oil from Citrus reticulata fruit peel (CR-EO). CR-EO was obtained by hydrodistillation in a Clevenger-type apparatus and its chemical composition was analyzed by GC-MS and GC-FID. Limonene (85.7%), ɣ-terpinene (6.7%) and myrcene (2.1%) were identified as its major components. CR-EO showed high activity against promastigote forms of Leishmania amazonensis (IC50 = 8.23 µg/mL). CR-EO also exhibited high larvicidal activity against third instar Aedes aegypti larvae at a lethal concentration (LC50 = 58.35 µg/mL) and 100% mortality at 150 µg/mL. This study suggests, for the first time, the potential use of CR-EO against this important mosquito-borne viral disease caused by the genus Aedes.

Foram coletadas quatro espécies de escorpiões na Mata do Buraquinho, João Pessoa, Paraíba, Brasil (Ananteris mauryi Lourenço, 1982, Tityus neglectus Mello-Leitão, 1932, T. pusillus Pocock, 1893 e T. stigmurus (Thorell, 1876)). Aspectos da biologia de A. mauryi e novos registros de distribuição geográfica das outras espécies foram anotados. Notas sobre a abundância e razão sexual de A. mauryi, a espécie mais abundante nessa área, são apresentados e discutidos.

Four species of scorpions (Ananteris mauryi Lourenço, 1982, Tityus neglectus Mello-Leitão, 1932, T. pusillus Pocock, 1893 and T. stigmurus (Thorell, 1876)) were collected in the " Mata do Buraquinho" , João Pessoa, state of Paraíba, Brazil. Aspects of biology for A. mauryi and new records on the geographic distribution of other species were annotated. Notes on the abundance and sex ratio on A. mauryi, the most abundant species in the area, are presented and discussed.

This work presents the experience of the Laboratory of Arthropods at Instituto Butantan, which maintains scorpions in captivity in order to obtain the venom used in the production of anti-arachnid serum. Between 1993 and 2000, the laboratory received 24.781 specimens of Tityus serrulatus in order to obtain poison for the production of anti-scorpion serum. In the first extraction, performed by electrical stimulation, the animals gave an average quantity of 0.4 mg venom per specimen. Animal acquisition and involved professional safety are considered. In addition, the captivity, handling and feeding techniques are described, as well as the method and equipment used for venom extraction. It shows the importance of regular campaigns, offering information to the general population in order to motivate these to catch and send alive scorpions to the Institute to assuring a regular entrance of scorpions.

Two scorpion species of medical importance occur in the state of São Paulo: the native brown scorpion Tityus bahiensis (Perty) and the highly toxic yellow scorpion Tityus serrulatus Lutz and Mello, which was brought into the state. The study of accident data of patients admitted to the Vital Brazil Hospital of the Butantan Institute from l982 to l993 revealed a gradual increase in stings by T. serrulatus in contrast to those by T. bahiensis with a variation from 5.2% to 29.7%. Also, an inventory of the scorpions sent to the Laboratory of Venomous Arthropods of the Butantan Institute over the same period showed an expressive growth in the number of T. serrulatus specimens in contrast to that of T. bahiensis. These data suggest a significative proliferation of T. serrulatus in the state of São Paulo over the past 12 years, and a probable interspecific competition between T. serrulatus and the endemic T. bahiensis. The analysis of scorpion accidents reported to the state of São Paulo Health Department (Secretaria de Estado da Saúde de São Paulo) corroborated the higher severity of T. serrulatus envenoming. The results pointed to the possibility of an increase in severe envenoming and deaths by scorpion stings in the state of São Paulo. Aiming to contribute to scorpion prevention and control programs, the present geographical distribution of both dangerous species was mapped, pointing to the areas of higher risk of scorpionism in the state.