Abstract The objective of this work was to develop innovative formulations of dulce de leche made with sheep's milk with and without the addition of sheep’s milk cream and evaluate the shelf life of these products. Physicochemical and microbiological evaluations were performed on sheep’s pasteurized milk and cream, as well as on dulce de leche right after production and during the storage time. Addition of cream to sheep dulce de leche did not change the physicochemical and microbiological parameters between formulations. The dulce de leche showed dark color and adequate results of protein, fat, total and microbiological counts (thermotolerant coliforms, Staphylococcus aureus, Salmonella sp. in all evaluations, and total mesophilic aerobic up to 150 days of storage). Innovative formulations of dulce de leche made by sheep’s milk were developed, differentiated from those existing in the Brazilian market, but a specific legislation is required in Brazil focused on the specific characteristics and quality parameters of sheep’s milk and its derivatives.

Resumo O objetivo deste trabalho foi determinar a concentração de sódio em queijos comercializados na região da fronteira oeste do Rio Grande do Sul. Foram coletadas 30 amostras de queijos (Parmesão ralado, Muçarela, Prato e Artesanal) de diferentes marcas e lotes, as quais foram analisadas em quadruplicata quanto ao conteúdo de sódio, empregando a espectroscopia de emissão atômica. A quantificação do teor de sódio permitiu constatar que houve diferença significativa entre os lotes de todas as marcas de queijos avaliadas. Os resultados indicaram que 44,4% das empresas estavam com os valores da rotulagem nutricional em desacordo com a legislação. Além disso, 77,8% das marcas não cumprem a meta para redução de sódio acordada entre o Ministério da Saúde e Associação Brasileira das Indústrias da Alimentação, a qual deveria ter sido alcançada até o final do ano de 2016. Em relação aos queijos Artesanais não foram registradas altas concentrações de sódio. O queijo Parmesão ralado destacou-se pelo elevado conteúdo de sódio em sua formulação (2014,56 mg/100 g) e também pelo aumento da adição deste mineral com o passar dos anos. Com isso, evidencia-se a negligência da indústria em relação aos consumidores, devido à ausência de leis para padronizar a adição de sal na preparação de queijos.

Abstract This study was done to establish the sodium concentration in the cheeses marketed in the western border region of the Rio Grande do Sul. A total of 30 cheese samples (grated Parmesan, Mozzarella, Prato and Artisanal) were collected from different brands and lots. After analyzing them under atomic emission spectroscopy, in quadruplicate, the sodium levels were ascertained. From the quantification of the sodium content significant differences were noted among the lots of all the cheese brands assessed. The results clearly revealed that 44.4% of the companies had listed nutritional values on the labels that were noncompliant with the legislation. Besides, 77.8% of the brands failed to reduce the sodium concentration to the levels jointly decided upon by the Ministry of Health and Brazilian Association of Food Industries, which should have been achieved ideally towards the end of 2016. While the Artisanal cheeses did not register high concentrations of sodium, the formulation of the grated Parmesan cheese was characterized by its high sodium content (2014.56 mg/100 g), as well as by its steady rise in sodium concentration over the years. This is indicative of the inattentiveness of the industry towards the consumers, as stringent laws to standardize the quantity of added salt in cheeses preparations are nonexistent.

The objective of this study was to obtain and evaluate physicochemical and functional properties of protein concentrates from Micropogonias furnieri produced by pH shifting process, using alkaline and acid solubilization followed by isoelectric precipitation of muscle proteins. The concentrates showed high protein content and the maxim solubility for the minced (96.5%), for the alkaline (97.5%) and acid (93.7%) extraction was obtained at pH 11.0. The water holding capacity of the alkaline concentrate resulted in a value same or superior to water holding capacity of the acid concentrate in all investigated values of pH. The oil holding capacity of alkaline and acid concentrates showed no significant difference between the studied processes.

The objective of this work was to obtain hydrolysates with different degrees of hydrolysis using Alcalase 2.4L® and to evaluate the effect of the enzyme [E] and substrate [S] concentration on the functional properties of the hydrolysates. It was obtained hydrolysates with hydrolysis degree values ranging from 12.2 to 43.7%. The values of solubility and water holding capacity were similar for the greater number of the hydrolysates with minimum values in the pH 5.0. The hydrolysis degree showed direct relationship with the solubility and indirect with the water holding capacity, oil holding capacity, emulsifying capacity and foaming capacity.

Este trabalho teve como objetivos elaborar pães enriquecidos com proteína de pescado para aumentar o conteúdo deste nutriente no produto e avaliar estes pães sensorial, tecnológica e físico-quimicamente. A proteína de pescado foi obtida a partir de cabrinha (Prionotus punctatus), espécie de baixo valor comercial. Para obtenção da polpa de cabrinha, a matéria-prima foi submetida a um processo de lavagem, seguido de secagem. Foram desenvolvidas cinco formulações de pão: 30, 40 e 50% de polpa lavada úmida (PU) e 3 e 5% de polpa lavada seca (PS) em base à farinha. Os pães foram avaliados sensorial (teste de ordenação, escala hedônica e perfil de atributos), tecnológica (notas das características internas, externas e volume específico) e físico-quimicamente (composição centesimal). O teste de perfil de atributos mostrou que o pão com 5% PS não diferiu significativamente (alfa = 0,05) do padrão para nenhum atributo avaliado. Todos pães apresentaram aceitação superior a 74%, no entanto ocorreu diminuição das notas das características internas e externas e do volume específico dos pães com o aumento da adição de polpa em todas as formulações, caracterizando queda da qualidade tecnológica. Os pães com 3 e 5% de PS e 50% de PU apresentaram um aumento no conteúdo protéico de 31, 45 e 48% respectivamente, em relação ao conteúdo protéico do pão padrão.

This work had as objectives to elaborate breads enriched with minced meat of fish and evaluate them sensorial, technological and physico-chemically. The fish protein was obtained from cabrinha (Prionotus punctatus), a low commercial value specie. To obtain the minced fish, the grinded meat was submitted to three wash steps with water, resulting the wet washed minced fish (WWMF). When the WWWF was dried and sifted, it was obtained the dry washed minced fish (DWMF). Five bread formulations with addition of 30, 40 and 50% of WWMF and 3 and 5% of DWMF were tested. All breads obtained were evaluated sensorial (preference test ranking, nine-point hedonic scale and attribute profile), technological (specific volume and technological score for the external and internal characteristics) and physico-chemically (centesimal composition). The attribute profile test showed that the bread containing 5% DWMF didn’t differ significantly (alpha = 0.05) from the bread without addition of fish protein in any attribute. All formulations showed an acceptance index higher than 74%, however it was observed a decreasing of technological score and specific volume values with the increasing amount of minced fish in all formulations, resulting technological quality loss. Breads containing 3 and 5% of DWMF and 50% of WWMF presented a protein increase of 31, 45 e 48%, respectively, when compared to the protein of the bread without addition of fish.