RESUMO Descrevem-se os aspectos clínicos, anatomopatológicos, imuno-histoquímicos, microbiológicos e moleculares de um caso de adenocarcinoma pulmonar associado à infecção por Mycobacterium sp. em uma vaca. O animal apresentou hiporexia, emagrecimento, vocalizações, postura ortopneica, ingurgitamento da jugular, estase venosa positiva, gemido expiratório e morte. Na necropsia, os pulmões estavam aumentados e apresentavam, na superfície pleural, nódulos branco-amarelados, firmes, multifocais a coalescentes, interpostos por áreas avermelhadas. Ao corte, os nódulos aprofundavam-se ao parênquima e possuíam múltiplos focos de aspecto caseoso e friável e áreas de mineralização. O saco pericárdico e os linfonodos traqueobrônquicos, ilíacos, lombares aórticos e mamários apresentavam lesões semelhantes. Histologicamente, observou-se neoformação carcinomatosa associada a áreas multifocais de necrose e mineralização. As células neoplásicas foram fortemente imunomarcadas pelo anticorpo antipancitoqueratina AE1/AE3. Na cultura microbiológica de fragmentos dos pulmões, houve crescimento de colônias bacterianas compatíveis com micobactérias atípicas. O sequenciamento molecular submetido ao BLASTn identificou o Mycobacterium sp. WCM 7299 (ID: gb|KJ873243.1|).

ABSTRACT The clinical, anatomopathological, immunohistochemical, microbiological and molecular aspects of a case of pulmonary adenocarcinoma associated with infection by Mycobacterium sp. in a cow are described. The animal presented hyporexia, weight loss, vocalizations, orthopneic posture, jugular engorgement, positive venous stasis, expiratory groaning and death. At necropsy, the lungs were enlarged and presented firm, multifocal to coalescent yellowish nodules, interposed by reddish areas on the pleural surface. At cut, the nodules deepened to the parenchyma and had multiple foci of caseous and friable appearance and areas of mineralization. The pericardial sac and tracheobronchial, iliac, aortic lumbar and mammary lymph nodes showed similar lesions. Histologically, a carcinomatous neoformation, associated with multifocal areas of necrosis and mineralization, was observed. Neoplastic cells were strongly immunolabelled by anti-PanCytokeratin antibody AE1/AE3. Microbiological culture of lung fragments showed growth of bacterial colonies compatible with atypical mycobacteria. Molecular sequencing submitted to BLASTn identified the Mycobacterium sp. WCM 7299 (ID: gb|KJ873243.1|).

Objective: To evaluate the impact of multidimensional interventions on quality of life (QoL) and depressive symptoms in Brazilian older adults living in the community. Methods: Longitudinal, quasi-experimental study of older adults receiving conventional primary health care (PHC). The interventions were designed in response to a first round of data collection and validated through pilot testing in groups of older adults from another community. The validated interventions were then applied to an intervention group (IG). To measure their effect, we used the Medical Outcomes Short-Form Health Survey (SF-36) quality of life scale and the Geriatric Depression Scale (GDS-30). Results: The sample comprised 118 participants. IG participants exhibited significant improvement in several QoL domains (SF-36): mental health (p = 0.010), general health perceptions (p = 0.016), and physical functioning (p = 0.045). No such improvement occurred in controls (p > 0.050). The prevalence of depression (GDS-30) fell from 36.7 to 23.3% in the IG, despite no significant difference (p = 0.272). Controls also reported a reduction in depressive symptoms, but only from 44.8 to 41.4% (p = 0.112). Conclusions: This multidimensional intervention was associated with significant improvement in mental health, general health perceptions, and physical functioning in a sample of Brazilian older adults. Clinical trial registration: RBR-92dbtx.

RESUMO O ajuste inadequado da sela é um dos principais causadores de lombalgias em equinos. A raça Mangalarga Marchador está entre as mais populares do Brasil, sendo muito utilizada para cavalgadas e prática de esportes. O presente estudo teve como objetivo avaliar termograficamente as selas utilizadas em cavalos dessa raça. Para tal, foram realizadas imagens termográficas de 18 selas de animais de diversas categorias durante uma Exposição Nacional do Cavalo Mangalarga Marchador. A avaliação foi realizada baseando-se em três parâmetros: simetria da área de contato, interação com a linha média dorsal e área total de contato com a pele (25%, 50%, 75% ou 100%). Em 83,3% das selas avaliadas foi observada assimetria da área de contato. A interação com a linha média dorsal foi observada com a mesma frequência. Apenas 22,2% das selas avaliadas no presente estudo tinham suadouros com área de contato maior que 50%. Com base nos resultados obtidos neste trabalho, conclui-se que a termografia é uma ferramenta útil na avaliação do contato das selas com o dorso dos cavalos e que existe alta frequência de inconformidades no ajuste de selas utilizadas na raça Mangalarga Marchador.

ABSTRACT Poor saddle-fitting is one of the main causes of back pain in horses. Mangalarga Marchador is a popular breed in Brazil, being used mainly for pleasure riding and sports. This study aimed to thermographically assess saddles used in horses of this breed. Thermographic images were obtained from 18 saddles of animals from different categories during a Mangalarga Marchador National Exposition. The evaluation was based on three parameters: contact area symmetry, dorsal midline interaction and total skin contact area (25%, 50%, 75% or 100%). Contact area asymmetry was observed in 83.3% of saddles. Dorsal midline interaction was observed with the same frequency. Only 22.2% of saddles assessed in the present study had panels with contact areas greater than 50%. Based on the results of this essay it can be concluded that thermography is a useful tool for the evaluation of saddle contact area with the back of horses and that there is a high frequency of fitting unconformities in saddles used in Mangalarga Marchador horses.

A podridão verde do inhame é uma doença de grande expressão econômica no estado de Pernambuco. Foram realizados estudos fisiológicos visando avaliar a influência de seis meios de cultura (BDA, Aveia, V-8, Czapeck, Cenoura e Inhame) sobre o crescimento micelial, esporulação e peso seco de dois isolados de Penicillium sclerotigenum (IB1 e IPR2). Os meios BDA e Inhame induziram maior crescimento micelial e, a maior produção de conídios foi obtida no Czapeck e Aveia, para ambos isolados utilizados. O meio de Aveia propiciou maior peso seco para o IB1 e IPR2. Foi estudado o efeito de diferentes combinações de fontes de carbono (maltose, dextrose, sacarose, amido) e nitrogênio (asparagina, peptona, nitrato de sódio, nitrato de potássio) sobre o desenvolvimento de P. sclerotigenum. O nitrato de sódio foi menos favorável ao crescimento micelial e esporulação. A combinação sacarose x peptona proporcionou o melhor crescimento micelial para ambos isolados. Para o IB1, a maior produção de conídios foi obtida com a sacarose x nitrato de potássio e para o IPR2 com a sacarose x peptona.

The yam green rot is a disease with high economic impact in Pernambuco state. It was carried out physiological studies involving six culture media (PDA, Oat-agar, V-8 juice, Czapeck, Carrot and Yam) on mycelial growth, sporulation and dry weight of two Penicillium sclerotigenum isolates (IB1 and IPR2). PDA and Yam media induced higher mycelial growth and higher conidium production was obtained in the Czapeck and Oat-agar for both isolates utilized. The Oat-agar media provided higher dry weight for IB1 and IPR2. It was also studied the effect of different carbon source (maltose, dextrose, sucrose, starch) and nitrogen (asparagin, pepton, sodium nitrate, potassium nitrate) on development of P. Sclerotigenum. Among the nitrogen sources evaluated, sodium nitrate was the less favorable for the mycelial growth and sporulation. The sucrose x pepton combination provided the best mycelial growth for both isolates. The highest conidium production was achieved using the combination of sucrose x potassium nitrate and sucrose x peptone for the isolates IB1 and IPR2, respectively.

Pressor responses elicited by stimulation of the nucleus raphe obscurus (NRO) depend on the integrity of the rostral ventrolateral medulla (RVLM). Therefore, to test the participation of excitatory amino acid (EAA) receptors in the cardiovascular responses evoked by NRO stimulation (1 ms, 100 Hz, 40-70 µA, for 10 s), the EAA antagonist kynurenic acid (Kyn) was microinjected at different sites in the ventrolateral medullar surface (2.7 nmol/200 nl) of male Wistar rats (270-320 g, N = 39) and NRO stimulation was repeated. The effects of NRO stimulation were: hypertension (deltaMAP = +43 ± 1 mmHg, P<0.01), bradycardia (deltaHR = -30 ± 7 bpm, P<0.01) and apnea. Bilateral microinjection of Kyn into the RVLM, which did not change baseline parameters, almost abolished the bradycardia induced by NRO stimulation (deltaHR = -61 ± 3 before vs -2 ± 3 bpm after Kyn, P<0.01, N = 7). Unilateral microinjection of Kyn into the CVLM did not change baseline parameters or reduce the pressor response to NRO stimulation (deltaMAP = +46 ± 5 before vs +48 ± 5 mmHg after Kyn, N = 6). Kyn bilaterally microinjected into the caudal pressor area reduced blood pressure and heart rate and almost abolished the pressor response to NRO stimulation (deltaMAP = +46 ± 4 mmHg before vs +4 ± 2 mmHg after Kyn, P<0.01, N = 7). These results indicate that EAA receptors on the medullary ventrolateral surface play a role in the modulation of the cardiovascular responses induced by NRO stimulation, and also suggest that the RVLM participates in the modulation of heart rate responses and that the caudal pressor area modulates the pressor response following NRO stimulation.

A podridão do colo do feijoeiro comum (Phaseolus vulgaris), causada por Sclerotium rolfsii, pode ocasionar grandes perdas na produção da cultura, já que as medidas de controle aplicadas para esta doença, não têm proporcionado resultados efetivos. Neste contexto, existe a necessidade de identificar fontes de resistência em cultivares e linhagens de feijoeiro a S. rolfsii. Os isolados foram obtidos de caules de plantas de feijoeiro infetadas, provenientes de diferentes sub-regiões do Estado de Pernambuco e a inoculação foi realizada por deposição de dez escleródios no colo das plantas, previamente ferido. A severidade da doença foi avaliada através de escala de notas variando de 1 a 9, proposta pelo CIAT, e os dados transformados para índice de doença de Mackinney. Todas as cultivares e linhagens mostraram-se suscetíveis aos isolados provenientes da Zona da Mata e Agreste, que foram considerados mais virulentos. As cultivares e linhagens que se mostraram resistentes ao isolado proveniente do Sertão foram: IPA-10, Corrente, Mão Curta, Gordo e L-96029, sendo este isolado considerado menos virulento.

Collar rot of common bean (Phaseolus vulgaris), caused by Sclerotium rolfsii, can induce high production losses. The control measures, commonly recommended for this disease, have not been providing effective results. The objective of the present work is to identify the sources of resistance to S. rolfsii in common beans. The isolates were obtained from the stem of infected plants, coming from different areas of the State of Pernambuco, and the inoculation was done by deposition of ten sclerotia on the collar of previously wounded plants. The severity of the disease was evaluated through note scale varying from 1 to 9, proposed by the CIAT and the data were transformed into the Mackinney disease index. All the cultivars and lines have shown susceptibility to the isolates from Zona da Mata and Agreste, which were considered more virulent. The cultivars and lines which showed resistance to the isolate from Sertão were: IPA-10, Corrente, Mão Curta, Gordo and L-96029, which wer considered less virulent.