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This report describes the fabrication of a low-cost magnetic separator holder combining 3D printing and compact neodymium blocks for allowing magnetic extraction and purification of RNA from samples collected by nasopharyngeal swab from patients infected by SARS-CoV-2. The device was designed to contain 24 entrances for plastic microtubes in an arrangement like a commercial device. The proof of concept of the proposed device was successfully demonstrated through the sample extraction and purification of swab samples collected from eight patients suspected of SARS-CoV-2 infection. The sample preparation protocol was performed using a commercial kit containing magnetic beads and different solutions. The performance of the printed device was compared to a commercial magnetic separator, usually employed in the golden standard techniques. The fabrication of the 3D printed magnetic separator was completed under optimized printing conditions within 6 h at cost of 4 USD per unit. The RNA extracted from samples using both devices was analyzed by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) and the achieved results have indicated no statistical different at confidence level of 95%. Based on the achievements, the use of 3D printing and neodymium blocks have demonstrated an alternative route to be used in routing analysis associated to COVID-19 diagnosis.

https://doi.org/10.21577/0100-4042.20230023

Body yield and quality of fresh and post-freezing filet of Nile tilapia (Oreochromis niloticus) genetic groups
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NUNES, ANDRÉ L.

; CORRÊA FILHO, RUY A.C.

; SERAPHIM, GUILHERME N.

; PIRES, LUANA B.

; MARTINS, THIAGO X.

; PRATES, MICHEL F.M.

; OLIVEIRA, CARLOS A.L.

; DUARTE, MARJORIE T.

; POVH, JAYME A.

Anais da Academia Brasileira de Ciências

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2023, Volumen 95 Nº 4 elocation e20190509

Resumen: En | Texto: En | PDF: En

Abstract The aim of this study was to evaluate the body yield and quality of fresh and post-freezing filet of male and female fish of inbred and non-inbred AquaAmérica genetic group and the hybrid between the AquaAmérica and Tilamax varieties. Forty fish (20 males and 20 females) of each genetic group were housed in four 48-m3 hapa net cages, getting 120 fish per cage. The fish were housed at 51 days of age and farmed for 269 days. Pre-slaughter weight was higher (P<0.05) in the AquaAmérica × Tilamax males (0.805±0.204 kg) than in the inbred AquaAmérica male (0.643±0.115 kg). Filet yield percentage was higher (P<0.05) in the AquaAmérica × Tilamax males (32.14±4.72%) than in the inbred AquaAmérica (28.15±2.67%) and non-inbred AquaAmérica (29.06±2.80%) males. Head and viscera yield percentages, pH, color values (L*, a* and b*), shear force, drip loss and cooking loss did not differ significantly between the genetic groups and sexes. Alterations in meat quality were observed after freezing. In conclusion, inbreeding in the AquaAmérica variety resulted in reduced slaughter weight for males; AquaAmérica × Tilamax males have a higher filet yield; and filet quality is not influenced by crossing, inbreeding, or sex, but is changed after freezing.

https://doi.org/10.1590/0001-3765202320190509

First list of parasitoids in flies of medical, veterinary, and forensic importance in Rio Grande do Sul – Brazil
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Cárcamo, M. C.

; Guidotti, L.

; Abreu, T. F.

; Egewarth, J.

; Duarte, J. P.

; Costa, V.

Brazilian Journal of Biology

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2022, Volumen 82 elocation e238772

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Moscas necrófagas possuem grande importância para a sanidade humana e animal. Onde estas se desenvolvem ocorrem parasitoides que podem ser utilizados como ferramentas para o controle desses dípteros. No Brasil, a fauna desses parasitoides vem sendo investigada em algumas regiões e no Rio Grande do Sul é conhecida a ocorrência de apenas uma espécie. Portanto, o objetivo deste estudo foi criar a primeira lista de espécies de parasitoides de moscas de importância médica e veterinária ocorrentes no extremo sul do Brasil. As coletas foram realizadas no município de Pelotas (31º 42’ S; 52º 18’ O). Foram montadas três réplicas de bandejas contendo, cada uma, 500 g de fígado bovino, expostas ao ar livre durante 20 dias. Na sequência, as pupas foram individualizadas e observadas até a emergência. Foram identificados 4.882 adultos pertencentes às famílias Calliphoridae, Fanniidae, Muscidae e Sarcophagidae. Emergiram 4.040 parasitoides, pertencentes a oito espécies, das quais Nasonia vitripennis, Spalangia cameroni, Spalangia chontalensis e Tachinephagus zealandicus são novas ocorrências para o Rio Grande do Sul. Foram coletadas três espécies ainda não descritas do gênero Aphaereta. Apesar de ainda ser um primeiro esforço de inventariar a fauna de himenópteros parasitoides, este estudo pode auxiliar no desenvolvimento de programas de manejos desses dípteros na região.

Abstract Necrophagous flies are of great importance for human and animal health. In places where their development occurs, parasitoids can be used as a tool to control these dipterans. In Brazil, the fauna of these parasitoids has been investigated in some regions. However, in Rio Grande do Sul, it is known the occurrence of only one species. Thus, this study aimed to create the first list of parasitoids in flies of medical and veterinary importance in Southern Brazil. Collections took place in the municipality of Pelotas (31º 42’ S; 52º 18’ W). Three replicates consisting of a 500 g bovine liver chunk placed in a tray were exposed to open air for 20 days. Then pupae were individualized and observed until their emergence. We identified 4,882 adult flies of Calliphoridae, Fanniidae, Muscidae, and Sarcophagidae. 4,040 parasitoids emerged, belonging to eight species, of which Nasonia vitripennis, Spalangia cameroni, Spalangia chontalensis, and Tachinephagus zealandicus are new records in Rio Grande do Sul state. Also, three undescribed species of the genus Aphaereta were collected. In spite of being the first attempt to inventory the fauna of hymenopteran parasitoids, this study may help in the development of management programs of these dipterans in the region.

https://doi.org/10.1590/1519-6984.238772 208 descargas

Effect of Mauritia flexuosa L. leaf extract on Staphylococcus aureus and Staphylococcus haemolyticus biofilms adhered to stainless steel surface
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Mariano, E. G. A.

; Michel, A. G. F.

; Morais-Costa, F.

; Conceição, B. S. O.

; Silvério, F. O.

; Arrudas, S. R.

; Nunes, Y. R. F.

; Pinto, M. S.

; Careli, R. T.

; Duarte, E. R.

Brazilian Journal of Biology

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2022, Volumen 82 elocation e251140

Resumen: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Staphylococcus spp. represents the main mastitis agents in ruminants and contaminants of milk due to their expressive capacity to make biofilms. The aims in this study was evaluate evaluated the antimicrobial activity of Mauritia flexuosa L. extracts against Staphylococcus spp. adhered to a stainless steel surface. Two isolates from cows with clinical mastitis were evaluated; one was identified as Staphylococcus aureus, and the other Staphylococcus haemolyticus. Additionally the ATCC 25923 strain, S. aureus from human was evaluated. The chemical profile obtained from gas chromatography revealed the presence of carbohydrates, organic acids, and flavonoids. The minimum bactericidal concentrations of the ethanolic extract (EE) and aqueous extract (AE) were 4.4 and 5.82 mg/mL, respectively. After EE treatment at 4.4 mg/mL for 2.5 min, total removal of mature biofilms grown on stainless steel coupons was observed (reduction by 3.85-4.81 log units). This extract from M. flexuosa shows potential as an effective sanitizer and may represent a natural alternative against Staphylococcus spp.

Resumo Bactérias do gênero Staphylococcus spp. são os principais agentes da mastite em ruminantes e contaminantes do leite devido à expressiva capacidade de formação de biofilmes. Neste estudo o objetivo foi avaliar a atividade antimicrobiana de extratos de Mauritia flexuosa L. (Buritizeiro) contra Staphylococcus spp. aderidos à superfície de aço inoxidável. Foram avaliados dois isolados de vacas com mastite clínica; o um isolado foi identificado como Staphylococcus aureus e o outro como Staphylococcus haemolyticus. Adicionalmente foi também avaliada a e a cepa S. aureus ATCC 25923 de origem humana. O perfil químico obtido por cromatografia gasosa revelou a presença de carboidratos, ácidos orgânicos e flavonóides. As concentrações bactericidas mínimas do extrato etanólico (EE) e do extrato aquoso (AE) foram 4,4 e 5,82 mg / mL, respectivamente. Após o tratamento com EE a 4,4 mg / mL por 2,5 min, foi observada remoção total de biofilmes maduros cultivados em cupons de aço inoxidável (redução de 3,85-4,81 unidades log). O EE de folhas de M. flexuosa apresenta potencial como um desinfetante eficaz e pode representar uma alternativa natural contra Staphylococcus spp.

https://doi.org/10.1590/1519-6984.251140

Investigation of Tritrichomonas foetus in cryopreserved bovine semen by culture and polymerase chain reaction
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Ribeiro, L.

; Silva, O.R.

; Duarte, F.C.

; Jesus, V.L.T.

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

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Oct 2021, Volumen 73 Nº 5 Paginas 1023 - 1028

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO A tricomonose genital bovina (TGB), uma doença infectocontagiosa causada pelo Tritrichomonas foetus, é transmitida por via venérea e fômites contaminados. Em machos a doença é assintomática e permanente. O agente já foi encontrado em amostras de sêmen e é capaz de permanecer viável quando congelado. Este trabalho teve por objetivo investigar a presença de T. foetus em 27 amostras de sêmen bovino comercial congelado, por meio de cultivo e reação em cadeia da polimerase (PCR). As amostras foram descongeladas em água a 37ºC; parte foi inoculada em tubo de ensaio contendo meio Diamond, incubada a 35ºC com consequente avaliação de crescimento e avaliada a cada 24 horas, via exame direto em microscópio, e a outra parte foi diluída em PBS para análise molecular. Após 10 dias de cultivo, todas as amostras foram negativas. Para a detecção molecular foi utilizado o kit Quick-DNA Miniprep (Zymo Research) sem proteinase K para extração do DNA. Os iniciadores utilizados na PCR foram TRF3 e TRF4. O resultado da PCR foi negativo para todas as amostras. Conclui-se que as amostras utilizadas foram realmente negativas para a presença do patógeno em ambos os métodos diagnósticos, o que comprovou a inocuidade do sêmen testado.

ABSTRACT Bovine Trichomoniasis (BT) is an infectious disease caused by Tritrichomonas foetus that can be transmitted either sexually or by fomites. In males, the disease is asymptomatic and permanent. T. foetus has been detected in semen samples where it is able to remain viable even when frozen. The objective of this study was to investigate the presence of T. foetus in 27 samples of commercial frozen bovine semen by culture and Polymerase Chain Reaction (PCR). Samples were thawed in water at 37°C. Part of the samples was inoculated in a test tube containing Diamond’s medium and incubated at 35°C. Growth was evaluated every 24 hours via direct examination under a microscope. The other part was placed in an Eppendorf tube and frozen for later molecular analysis. After 10 days of culture, all samples were negative for T. foetus. The Quick-DNA Miniprep Kit (Zymo Research) without proteinase K was used for DNA extraction. The primers used in PCR were TRF3 and TRF4. PCR results were negative for all samples. In conclusion, bovine semen samples were negative for T. foetus in both diagnostic methods, according to the adopted methodology.

https://doi.org/10.1590/1678-4162-12370 43 descargas

Control of the fungi Lasiodiplodia theobromae, the causal agent of dieback, in cv. syrah grapevines
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Rusin, Carine

; Cavalcanti, Fábio Rossi

; Lima, Patrícia Carla Giloni de

; Faria, Cacilda Márcia Duarte Rios

; Almança, Marcus André Kurtz

; Botelho, Renato Vasconcelos

Acta Scientiarum. Agronomy

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2021, Volumen 43 elocation e44785

Resumen: En | Texto: En | PDF: En

ABSTRACT. Grapevine trunk diseases are among the most important limiting factors of worldwide viticulture. In this context, we aimed to verify the effect of chemical fungicides, biological agents and plant extracts on the control of Lasiodiplodia theobromae in pruning wounds and their physiological effects in cv. Syrah grapevines. Plant extracts (clove, cinnamon, garlic, rosemary and lemongrass), commercial fungicides (difeconazole, tebuconazole, mancozeb, sulfur, pyraclostrobin, fosetyl-Al, and azoxystrobin), chitosan, Trichoderma harzianum and Bacillus subtilis were used for the in vitro trials. The protection of pruning wounds in the potted vines was studied using fosetyl-Al, tebuconazole, Trichoderma harzianum, Bacillus subtilis, garlic extract and clove extract. The experiments were carried out through two vegetative cycles: 2015/2016 and 2016/2017. The length of wood discoloration, pathogen re-isolation percentage, fresh mass of the pruning material, peroxidase activity, fluorescence, chlorophyll index, phenological stages, shoot length and leaf area were evaluated. The treatments with clove, garlic extract, tebuconazole, pyraclostrobin, mancozeb, fosetyl-Al and B. subtilis reduced mycelial growth by more than 90%. In the grapevines, the use of T. harzianum decreased the re-isolation of L. theobromae, but no differences were verified for the other plant evaluations. We concluded that the use of T. harzianum would be a potential option for wound protection without altering the physiological aspects of cv. Syrah grapevines.

https://doi.org/10.4025/actasciagron.v43i1.44785 1038 descargas

Persistent symptoms and decreased health-related quality of life after symptomatic pediatric COVID-19: A prospective study in a Latin American tertiary hospital
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Fink, Thais T.

; Marques, Heloisa H.S.

; Gualano, Bruno

; Lindoso, Livia

; Bain, Vera

; Astley, Camilla

; Martins, Fernanda

; Matheus, Denise

; Matsuo, Olivia M.

; Suguita, Priscila

; Trindade, Vitor

; Paula, Camila S.Y.

; Farhat, Sylvia C.L.

; Palmeira, Patricia

; Leal, Gabriela N.

; Suzuki, Lisa

; Odone Filho, Vicente

; Carneiro-Sampaio, Magda

; Duarte, Alberto José S.

; Antonangelo, Leila

; Batisttella, Linamara R.

; Polanczyk, Guilherme V.

; Pereira, Rosa Maria R.

; Carvalho, Carlos Roberto R.

; Buchpiguel, Carlos A.

; Latronico, Ana Claudia

; Seelaender, Marilia

; Silva, Clovis Artur

; Pereira, Maria Fernanda B.

; Sallum, Adriana M. E.

; Brentani, Alexandra V. M.

; Neto, Álvaro José S.

; Ihara, Amanda

; Santos, Andrea R.

; Canton, Ana Pinheiro M.

; Watanabe, Andreia

; Santos, Angélica C. dos

; Pastorino, Antonio C.

; Franco, Bernadette D. G. M.

; Caruzo, Bruna

; Ceneviva, Carina

; Martins, Carolina C. M. F.

; Prado, Danilo

; Abellan, Deipara M.

; Benatti, Fabiana B.

; Smaria, Fabiana

; Gonçalves, Fernanda T.

; Penteado, Fernando D.

; Castro, Gabriela S. F. de

; Gonçalves, Guilherme S.

; Roschel, Hamilton

; Disi, Ilana R.

; Marques, Isabela G.

; Castro, Inar A.

; Buscatti, Izabel M.

; Faiad, Jaline Z.

; Fiamoncini, Jarlei

; Rodrigues, Joaquim C.

; Carneiro, Jorge D. A.

; Paz, Jose A.

; Ferreira, Juliana C.

; Ferreira, Juliana C. O.

; Silva, Katia R.

; Bastos, Karina L. M.

; Kozu, Katia

; Cristofani, Lilian M.

; Souza, Lucas V. B.

; Campos, Lucia M. A.

; Silva Filho, Luiz Vicente R. F.

; Sapienza, Marcelo T.

; Lima, Marcos S.

; Garanito, Marlene P.

; Santos, Márcia F. A.

; Dorna, Mayra B.

; Aikawa, Nadia E.

; Litvinov, Nadia

; Sakita, Neusa K.

; Gaiolla, Paula V. V.

; Pasqualucci, Paula

; Toma, Ricardo K.

; Correa-Silva, Simone

; Sieczkowska, Sofia M.

; Imamura, Marta

; Forsait, Silvana

; Santos, Vera A.

; Zheng, Yingying

Clinics

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2021, Volumen 76 elocation e3511

Resumen: En | Texto: En | PDF: En

OBJECTIVES: To prospectively evaluate demographic, anthropometric and health-related quality of life (HRQoL) in pediatric patients with laboratory-confirmed coronavirus disease 2019 (COVID-19) METHODS: This was a longitudinal observational study of surviving pediatric post-COVID-19 patients (n=53) and pediatric subjects without laboratory-confirmed COVID-19 included as controls (n=52) was performed. RESULTS: The median duration between COVID-19 diagnosis (n=53) and follow-up was 4.4 months (0.8-10.7). Twenty-three of 53 (43%) patients reported at least one persistent symptom at the longitudinal follow-up visit and 12/53 (23%) had long COVID-19, with at least one symptom lasting for >12 weeks. The most frequently reported symptoms at the longitudinal follow-up visit were headache (19%), severe recurrent headache (9%), tiredness (9%), dyspnea (8%), and concentration difficulty (4%). At the longitudinal follow-up visit, the frequencies of anemia (11% versus 0%, p=0.030), lymphopenia (42% versus 18%, p=0.020), C-reactive protein level of >30 mg/L (35% versus 0%, p=0.0001), and D-dimer level of >1000 ng/mL (43% versus 6%, p=0.0004) significantly reduced compared with baseline values. Chest X-ray abnormalities (11% versus 2%, p=0.178) and cardiac alterations on echocardiogram (33% versus 22%, p=0.462) were similar at both visits. Comparison of characteristic data between patients with COVID-19 at the longitudinal follow-up visit and controls showed similar age (p=0.962), proportion of male sex (p=0.907), ethnicity (p=0.566), family minimum monthly wage (p=0.664), body mass index (p=0.601), and pediatric pre-existing chronic conditions (p=1.000). The Pediatric Quality of Live Inventory 4.0 scores, median physical score (69 [0-100] versus 81 [34-100], p=0.012), and school score (60 [15-100] versus 70 [15-95], p=0.028) were significantly lower in pediatric patients with COVID-19 at the longitudinal follow-up visit than in controls. CONCLUSIONS: Pediatric patients with COVID-19 showed a longitudinal impact on HRQoL parameters, particularly in physical/school domains, reinforcing the need for a prospective multidisciplinary approach for these patients. These data highlight the importance of closer monitoring of children and adolescents by the clinical team after COVID-19.

https://doi.org/10.6061/clinics/2021/e3511

Os Percentis e Pontos de Corte da Circunferência Abdominal para Obesidade em uma Ampla Amostra de Estudantes de 6 a 10 Anos de Idade do Estado de São Paulo, Brasil
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Santos, José Luiz F

; Valério, Valentin P.

; Fernandes, Rafael N.

; Duarte, Ligia

; Assumpção, Antonio C.

; Guerreiro, Jayme

; Sickler, Antonio L.

; Lemos, Álvaro A. R.

; Goulart Filho, Jayro G

; Cesar, Luiz Antonio Machado

; Pinto, Ibraim Masciarelli

; Magalhães, Carlos

; Hussid, Maria Fernanda

; Camacho, Cleber

; Avezum, Alvaro

; Sangaleti, Carine T.

; Consolim-Colombo, Fernanda Marciano

Arquivos Brasileiros de Cardiologia

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Mar 2020, Volumen 114 Nº 3 Paginas 530 - 537

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Fundamento A prevalência da obesidade vem aumentando sistematicamente na população, inclusive nas crianças e adolescentes, ao redor do mundo. Objetivos Descrever curvas percentílicas de referência para a circunferência abdominal (CA) nas crianças brasileiras e fornecer pontos de corte da CA para identificar crianças com risco de obesidade. Métodos Um estudo multicêntrico, prospectivo, tranversal foi realizado em crianças com idades entre 6 e 10 anos, matriculadas no ensino fundamental de escolas públicas e particulares de 13 cidades do estado de São Paulo. A estatura, o peso e a CA foram medidos em duplicata em 22.000 crianças (11.199 meninos). Para estabelecer o melhor ponto de corte da CA para o diagnóstico da obesidade, foram calculadas curvas ROC com crianças classificadas como com peso normal e obesas, de acordo com as curvas do IMC, estratificadas por gênero e idade, e o índice Youden foi utilizado como a eficácia potencial máxima desse biomarcador. Valores de p < 0,05 foram considerados estatisticamente significativos. Resultados Os valores da CA aumentaram com a idade, tanto nos meninos quanto nas meninas. A prevalência de obesidade em cada grupo variou de 17% (6 anos de idade) a 21,6 % (9 anos de idade), dentre os meninos, e de 14,1% (7 anos de idade) a 17,3 % (9 anos de idade), dentre as meninas. As análises ROC mostraram o percentil 75 como ponto de corte para o risco de obesidade, e o diagnóstico de obesidade está classificado no percentil 85 ou acima. Conclusão Curvas de referência da CA específicas para idade e sexo em crianças brasileiras e pontos de corte para o risco de obesidade podem ser usados em triagem nacional e estudos intervencionais para reduzir a carga da obesidade no Brasil. (Arq Bras Cardiol. 2020; 114(3):530-537)

Abstract Backgroud The prevalence of obesity has systematically been increased in the population, including children and adolescents, around the world. Objectives To describe reference percentile curves for waist circumference (WC) in Brazilian children and provide cut-off values of WC to identify children at risk for obesity. Methods A multicenter, prospective, cross-sectional study was performed with children aged from 6 to 10 years old, enrolled in public and private elementary schools from 13 cities of the São Paulo State. Height, weight, and WC were measured in duplicate in 22,000 children (11,199 boys). To establish the WC best cut-off value for obesity diagnosis, ROC curves with children classified as normal weight and obese were calculated, according to BMI curves, stratified by gender and age, and the Youden Index was utilized as the maximum potential effectiveness of this biomarker. A p < 0.05 was considered statistically significant. Results WC values increased with age in both boys and girls. The prevalence of obesity in each age group varied from 17% (6 years old) to 21.6% (9 years old) among boys, and from 14.1% (7 years old) to 17.3 % (9 years old) among girls. ROC analyses have shown the 75th percentile as a cut-off for obesity risk, and the diagnosis of obesity is classified on the 85th percentile or more. Conclusion Age and gender specific reference curves of WC for Brazilian children and cut-off values for obesity risk may be used for national screening and interventional studies to reduce the obesity burden in Brazil. (Arq Bras Cardiol. 2020; 114(3):530-537)

https://doi.org/10.36660/abc.20190043 2020 descargas

ETHANOLIC AND HYDROALCOHOLIC EXTRACTS OF PITANGA LEAVES (Eugenia uniflora L.) AND THEIR FRACTIONATION BY SUPERCRITICAL TECHNOLOGY
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Garmus, Tábata T.

; Kopf, Sarah F. M.

; Paula, Julia T.

; Aguiar, Ana C.

; Duarte, Gustavo H. B.

; Eberlin, Marcos N.

; Cabral, Fernando A.

Brazilian Journal of Chemical Engineering

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Jun 2019, Volumen 36 Nº 2 Paginas 1041 - 1051

Resumen: En | Texto: En | PDF: En

Abstract Hydroalcoholic extracts prepared by conventional methods (in stirred vessel at 25 °C and 1 bar) and, hydroalcoholic and supercritical carbon dioxide (scCO2) extracts prepared in fixed bed extractors (60 ºC and 400 bar) were obtained from pitanga leaves (Eugenia uniflora L.) in order to recover phenolic compounds. Hydroalcoholic and ethanolic extracts obtained in fixed bed were further fractionated in four separators by gradual pressure reduction, using scCO2 as an antisolvent. All extracts and fractions were characterized in terms of extraction yield and the presence of total phenolics and flavonoids. Solvents were found to significantly influence the extraction yields and composition. Yields increased as a function of polarity, whereas the hydroalcoholic extractions displayed the highest yields and the highest amounts of extracted phenols and flavonoids.

https://doi.org/10.1590/0104-6632.20190362s20180159 1440 descargas

10.

Zika Virus Activity of the Leaf and Branch Extracts of Tontelea micrantha and Its Hexane Extracts Phytochemical Study
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Ferreira, Fernanda L.

; Hauck, Marcela S.

; Duarte, Lucienir P.

; Magalhães, José C. de

; Silva, Louise S. M. da

; Pimenta, Lúcia P. S.

; Lopes, Julio C. D.

; Mercadante-Simões, Maria O.

; Vieira Filho, Sidney A.

Journal of the Brazilian Chemical Society

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Abr 2019, Volumen 30 Nº 4 Paginas 793 - 803

Resumen: En | Texto: En | PDF: En

The new triterpene friedelan-1,3,21-trione, the known compounds friedelan-3-one, 3β-friedelinol, 3,4-seco-friedelan-3-oic acid, 28-hydroxyfriedelan-3-one, friedelan-3-oxo-28-al, friedelan-3,21-dione, 30-hydroxyfriedelan-3-one, a mixture of 30-hydroxyfriedelan-3-one/21α-hydroxyfriedelan-3-one, 21β-hydroxyfriedelan-3-one, gutta-percha, squalene, and a mixture of palmitic/stearic/oleic acids were isolated from the hexane extracts of leaves and branches of T. micrantha. Their chemical structures were established by Fourier transform infrared spectroscopy (FTIR), gas chromatography (GC), 1D/2D nuclear magnetic resonance (NMR) and comparison with the literature data. All compounds were described for T. micrantha and the genus Tontelea for the first time. The branch and leaf extracts displayed anti-Zika virus activity at the lowest tested concentration of 15.6 µg mL-1, mainly virucidal effect, and presented no cytotoxicity to Vero cells. Furthermore, the ethyl acetate and methanolic leaf extracts demonstrated the best activities at the concentration of 31.2 and 15.6 µg mL-1 at the viral adsorption and penetration stages, respectively. These results showed that these extracts may be promising candidates for the Zika virus treatment.

https://doi.org/10.21577/0103-5053.20180210 729 descargas

11.

LUCLARICIN, A NEW LIGNAN FROM PHYLLANTHUS ACUMINATUS
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Duarte, Susyanne L. F.

; Nascimento, Yuri M.

; Madeiro, Sara A. L.

; Costa, Vicente C. O.

; Agra, Maria F.

; Sobral, Marianna V.

; Braz-Filho, Raimundo

; Carvalho, Mario G.

; Carvalho, João E.

; Ruiz, Ana L. T. G.

; Turri, Larissa S.

; Silva, Marcelo S.

; Tavares, Josean F.

Química Nova

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Ago 2018, Volumen 41 Nº 8 Paginas 880 - 883

Resumen: En | Texto: En | PDF: En

The secondary metabolites of the aerial parts of Phyllanthus acuminatus Vahl (Phyllanthaceae) and the biological activity of one of them were characterized in this study. This chemical investigation led to isolation of two lignans, luclaricin (1), a new arylnaphtalene-typelignan, and justicidin B (2). The structures of isolated compounds were determined based on 1D and 2D-NMR, HRESIMS and IR. The cytotoxic activity of justicidin B was assessed against tumor cell lines (MCF-7, U251, NCI-ADR/RES, NCI-H460, OVCAR-3, HT29 and K562) and a non-tumor cell line (HaCat) using sulforhodamine B assay. Justicidin B showed strong cytotoxic activity against the tumor cell lines HT29, K562 and U251 with GI50 values of 2.28, 3.29 and 5.76 mol L-1, respectively.

https://doi.org/10.21577/0100-4042.20170266 546 descargas

12.

Synthesis, DNA Interaction and Genotoxic Evaluation of a Photoactive Benzothiadiazole with Potential Application in Photovoltaic Paint
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Damiani, Adriani P.

; Rohr, Paula

; Westrup, José L.

; Duarte, Rodrigo C.

; Longaretti, Luiza M.

; Rocha, Bruna M. da

; Strapazzon, Giulia

; Venturini, Lara C.

; Souza, Emanuel

; Pich, Claus T.

; Frizon, Tiago E. A.

; Andrade, Vanessa M. de

; Rodembusch, Fabiano S.

; Dal-Bó, Alexandre G.

Journal of the Brazilian Chemical Society

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Jul 2018, Volumen 29 Nº 7 Paginas 1388 - 1399

Resumen: En | Texto: En | PDF: En

A new fluorophore containing 2,1,3-benzothiadiazole unit with alkylated tetrazole terminal groups as electron donors connected through a π-conjugated system of the type (D-π-A-π-D) was synthesized using the Sonogashira coupling reaction with good yields. The genotoxicity of the two intermediate compounds and the final product was evaluated in vitro in consideration of the potential application of the new compound in the chemical production of photovoltaic paints. The new fluorophore did not show significant biological effects in the cell viability test and the comet assay when compared with the intermediate compounds that were obtained synthetically despite the ability to interact with deoxyribonucleic acid (DNA) as demonstrated in circular dichroism assays. Thus, the compound does not show toxic or genotoxic activity, and therefore, it can be applied as a pigment in photovoltaic paint.

https://doi.org/10.21577/0103-5053.20170241 383 descargas

13.

Chemical characterization, hardness and termite resistance of Quercus cerris heartwood from Kosovo
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Bajraktari, A.

; Nunes, L.

; Knapic, S.

; Pimenta, R.

; Pinto, T.

; Duarte, S.

; Miranda, I.

; Pereira, H.

Maderas. Ciencia y tecnología

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Jul 2018, Volumen 20 Nº 3 Paginas 305 - 314

Resumen: En | Texto: En | PDF: En

Abstract: Quercus cerris, the Turkey oak, is an oak species native to southern central and southeastern Europe, extending into southwestern Asia. It is present in a large extent in the forests of the Republic of Kosovo, and at present used mainly for bio-energy. The potential use of Q. cerris wood for construction and higher value wood products has been investigated in the last years. However limited information is available on wood characteristics and performance, mainly regarding chemical composition and durability. The heartwood of Q. cerris taken from 70-90 year-old trees grown in two sites in Kosovo was studied regarding resistance to termite attack, chemical composition and hardness. The heartwood contained only 6,7% extractives, with a small content of tannins. The wood density was on average 0,81 at 12% moisture content and Brinell hardness 36,2 N mm-2. It was classified as not durable against subterranean termites and therefore not suitable for external use in ground contact in termite areas unless adequately protected. Nevertheless, Q. cerris wood showed adequate hardness and density for interior uses like flooring for domestic and commercial applications with moderate use.

593 descargas

14.

Correlations between caregiver psychiatric symptoms and offspring psychopathology in a low-resource setting
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Matsuzaka, Camila T.

; Wainberg, Milton L.

; Norcini Pala, Andrea

; Hoffmann, Elis V.

; Coimbra, Bruno M.

; Braga, Rosaly F.

; Duarte, Cristiane S.

; Sweetland, Annika C.

; Mello, Marcelo F.

Brazilian Journal of Psychiatry

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Ene 2018, Volumen 40 Nº 1 Paginas 56 - 62

Resumen: En | Texto: En | PDF: En

Objective: Associations between parental/caregiver depression and adverse child outcomes are well established and have been described through one or more mechanisms: child psychopathology following exposure to a depressed caregiver, child psychopathology exacerbating a caregiver’s depression, and caregiver and offspring depression sharing the same etiology. Data from low and middle-income countries is scarce. We examined correlations between common symptoms of mental disorders in caregivers and their offspring’s psychopathology in a Brazilian sample. Methods: In this cross-sectional study, adult caregivers were screened for depression during routine home visits by community health workers as part of the Brazilian Family Health Strategy. Caregivers with suspected depression were assessed using the Zung Self-Rating Depression Scale and the Self-Reporting Questionnaire (SRQ-20). Children’s symptoms were evaluated using the Strengths and Difficulties Questionnaire (SDQ). Results: The sample included 68 primary caregivers and 110 children aged 6 to 15 years. Higher caregiver scores on the SRQ-20 correlated significantly with psychiatric symptoms in offspring. Conclusion: These results substantiate our hypothesis that child psychopathology correlates with caregivers’ psychiatric symptoms. This paper adds to the growing literature on community mental health assessment and can help guide future strategies for reducing the burden of common mental disorders in caregivers and children alike in low and middle-income countries.

https://doi.org/10.1590/1516-4446-2016-1990 1035 descargas

15.

Intraspecific variation in seed size and light intensity affect seed germination and initial seedling growth of a tropical shrub
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Veloso, Aniele C. R.

; Silva, Priscila S.

; Siqueira, Wallison K.

; Duarte, Karen L.R.

; Gomes, Ingrid L. V.

; Santos, Henrique T.

; Fagundes, Marcilio

Acta Botanica Brasilica

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Dic 2017, Volumen 31 Nº 4 Paginas 736 - 741

Resumen: En | Texto: En | PDF: En

ABSTRACT Seed germination and seedling performance are affected by environmental factors and seed traits. In this study we investigated the effects of seed size and light intensity on germinability and seedling development of Copaifera oblongifolia. A total of 225 seeds were individually weighed and sown in three germination trays composed of 75 cells each. Each tray was placed in a different germination chamber with controlled photoperiod, temperature and light intensity. Seed size showed a positive relationship with time required for seed germination, and seeds exposed to high light intensity required more time to germinate. Seed size did not affect germination percentage, but seeds sown under high light intensity had a lower germination percentage than seeds sown under low light intensity and darkness. Seedling shoot mass showed a positive relationship with seeds mass, and seedlings grown in high light intensity had greater shoot mass than seedling growth in low light intensity and darkness. Thus, seed germinability of C. oblongifolia was higher in darkness while seedlings exhibited greater development under light. Looking to explain the ability of C. oblongifolia to colonize open/disturbed sites, it seems possible that plowing soil can bury seeds, thereby stimulating the germination of seeds present in the seed bank.

https://doi.org/10.1590/0102-33062017abb0032 5276 descargas

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