Abstract The “permanent tomato”, “spotted tuber” or “zebra chip” in potato and “chlorotic buds” of chili, are three diseases described in Mexico with coincidental signs of flower abortion, obscuration of vascular tissue at the base of the stem and root of the plants. The association between these diseases and the emerging bacterium Candidatus Liberibacter solanacearum (CLs) as well as the psyllid Bactericera cockerelli as its vector has been mentioned. These diseases, which were initially located in three states of Mexico, have spread to the main Solanaceae producing regions, both in field conditions and in the greenhouse. The objective of the study was to know the presence of CLs associated with diseases that affect the tomato, para and chili crops in Mexico. The bacterium was identified by PCR of the 16S rDNA gene, cloning and sequencing. The alignment of nucleotide sequences was performed with the Clustal W method and the phylogenetic tree was built with the Neighbor-Joining algorithm from distances calculated with the Tajima-Nei method and a Felsenstein index of 1 000 replicas, using the software MEGA version 5.05. A total of 167 samples were analyzed, of which 86 were positive, from 14 states of Mexico. Five nucleotide sequences from Guanajuato, San Luis Potosi and Sinaloa corresponding to tomato, potato and chili, adults and eggs of B. cockerelli were obtained. The analysis of the sequences showed an identity of 99.4% when compared between these and up to 99.8% with GenBank accessions described for CLs in the USA, New Zealand and Canada.

Resumen El “permanente del tomate”, “manchado del tubérculo” o “zebra chip” en papa y “brotes cloróticos” del chile, son tres enfermedades descritas en México con signos coincidentes de aborto de flor, oscurecimiento de tejido vascular en la base del tallo y raíz de las plantas. Se ha mencionado la asociación entre estas enfermedades y la bacteria emergente Candidatus Liberibacter solanacearum (CLs) así como al psílido Bactericera cockerelli como su vector. Estas enfermedades, que en inicio se localizaban en tres estados de México se han diseminado a las principales regiones productoras de solanáceas, tanto en condiciones de campo como en invernadero. El objetivo del estudio fue conocer la presencia de CLs asociado a enfermedades que afectan los cultivos de tomate, para y chile en México. La bacteria se identificó por PCR del gen 16S de ADNr, clonación y secuenciación. La alineación de secuencias nucleotídicas se realizó con el método Clustal W y el árbol filogenético se construyó con el algoritmo de Neighbor-Joining a partir de distancias calculadas con el método de Tajima-Nei y un índice de Felsenstein de 1 000 réplicas, utilizando el software MEGA versión 5.05. En total se analizaron 167 muestras, de las cuales 86 resultaron positivas, provenientes de 14 estados de México. Se obtuvieron cinco secuencias nucleotídicas de Guanajuato, San Luis Potosí y Sinaloa correspondientes al tomate, papa y chile, adultos y huevecillos de B. cockerelli. El análisis de las secuencias mostró una identidad de 99.4% al comparase entre estas y hasta 99.8% con accesiones del GenBank descritas para CLs en EUA, Nueva Zelanda y Canadá.

A lenta mudança da natureza da diferença de gênero responde tanto a mudanças sociais, como urbanização, educação, o ingresso das mulheres na força de trabalho e a democratização, quanto a esforços combinados por movimentos sociais e líderes políticos para a ampliação dos direitos da mulher. Esses fatores claramente tiveram impacto na República Dominicana na última década, influenciando as diferenças de gênero no país. Avaliamos a natureza e a evolução das diferenças de gênero na Rep. Dominicana mo período de 1994 a 2004, utilizando dados de surveys nacionais - Demos surveys - realizados em 1994, 1997, 2001 e 2004. A análise desses surveys indica que aspectos do que tem sido denominado uma 'diferença de gênero tradicional' permanece no país com relação ao engajamento cívico, interesse político e atitudes democráticas. Ao mesmo tempo, essa diferença desaparece com relação à participação eleitoral , e uma 'diferença moderna de gênero' emerge pela primeira vez com relação às atitudes sobre o papel da mulher na política. Idade e escolaridade têm efeitos diferenciais significativos e substantivos no conjunto de atitudes de homens e mulheres dominicanos nesse período.

The slowly changing nature of the gender gap responds both to significant social changes, such as urbanization, education, the entry of women into the labor force, and democratization, as well as to concerted efforts by social movements and political leaders to broaden women's right. These factors have clearly played an important role in the Dominican Republic over this past decade, impacting the country's gender gap. We assess the nature and evolution of the Dominican Republic's gender gap over the 1994-2004 period employing data taken from four nationwide public opinion surveys in the Dominican Republic - the Demos surveys - conducted in 1994, 1997, 2001, and 2004. The analysis of these surveys indicates that elements of what has been termed a traditional gender gap remain in place in the country with regard to civic engagement, political interest, and attitudes toward democracy. At the same time, this gap disappeared with regard to voter participation in elections and a modern gender gap emerged for the first time with regard to attitudes about the role of women in politics. Age and education have consistent and substantial differential effects across the attitudinal profiles of Dominican men and women over this time period.