A campilobacteriose é uma infecção frequentemente adquirida pelo consumo de produtos de origem animal. O frango é o principal responsável na cadeia de transmissão da enfermidade. A radiação ionizante foi utilizada para verificar a redução da carga bacteriológica de Campylobacter jejuni presente em fígados de frango, que podem apresentar, in natura, contaminação em até 100% dos casos. As doses de irradiação utilizadas foram 0,20 kGy, 0,27 kGy, 0,30 kGy e 0,35 kGy, sendo as amostras adquiridas em aviário, supermercado de bairro, supermercado popular e supermercado de grande porte. O processamento das amostras foi feito no Setor de Campylobacter, FIOCRUZ, e as irradiações feitas na COPPE na UFRJ, com irradiador gama provido de fonte de Cobalto 60. Somente a amostra congelada, adquirida em supermercado de bairro, não continha a bactéria. Em todas as outras amostras, pôde-se observar a presença de Campylobacter sp., mesmo quando usados procedimentos e tecnologias que visam impedir sua presença no alimento e, consequentemente, proteger a saúde humana. Alguns resultados se apresentaram abaixo do esperado, porém é sabido que as condições de crescimento exigidas por esta bactéria são incomuns quando comparadas às de outros enteropatógenos.

Campylobacteriosis is an infection frequently acquired through the consumption of animal origin products. Chicken can be considered the main responsible cause in the transmission chain of this disease. Ionizing radiation was used to verify the reduction of the microbiological load of Campylobacter jejuni present in chicken liver, which, in natura, can present contamination in up to 100% of the cases. The doses of irradiation used were: 0.20 kGy, 0.27 kGy, 0.30 kGy and 0.35 kGy. The samples of chicken liver were acquired in aviaries, local supermarkets and large chain supermarkets. The samples were analyzed for Campylobacter at FIOCRUZ. Irradiation was performed at COPPE/UFRJ, using a Gamma Cell Irradiator with a 60Co gamma source. Only the frozen sample acquired at the local supermarket did not contain the bacterium. Campylobacter sp. was present in all other samples, even when using procedures and technologies that aimed at the impediment of the presence of this bacterium in food and, consequently, at the protection of human health. On the whole, the results were satisfactory; nevertheless, it is known that the bacterial growth conditions required by this bacterium are uncommon when compared to other enteropathogenic bacteria.

Campylobacteriosis is an extremely important zoonosis, circulating freely in the environment. In nonhuman primates kept in open facilities and bred for experimental purposes, the presence of Campylobacter spp. could cause severe damage to the production and interfere with the results of scientific research. In this paper, we assessed the circulation of Campylobacter spp. in a colony of clinically healthy rhesus monkeys (Macaca mulatta) destined to research. The analysis was carried out during seven non-consecutive years. Data showed that despite several changes made in animal management along the studied years in order to control this zoonosis, reduction of bacterial charge did not occur. Significant differences among the age groups and sex were observed. Infants showed higher susceptibility than adult animals. In general males were more infected than females. Modifications adopted in the handling techniques need to be reviewed with the intent of improving the production, reducing bacterial infection of the stock and avoiding undesirable cross reactions in the research carried out with these animals. Therefore, this paper alerts professionals that work directly with captive rhesus monkeys about the risks of Campylobacter spp. infection and possible interference on the experimental procedures.

A multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.