Compaction processes are generally described using methods that do not distinguish volume changes of different types and pore sizes. The adjustment of shrinkage curves (ShC) using the XP model allows elucidation of the effects of soil management on changes in pore volume. This method coupled with micromorphological and micromorphometric determinations in soil thin sections allows the characterization and quantification of important soil features included pore types. The aim of this work was to examine the potential of shrinkage analysis to describe soil physical degradation under the most common management (continuous cropping-CC), an alternative management (integrated crop/livestock-ICL), related to quasi-pristine (QP); in two soils of northern of the Pampean region of Argentina (Typic Hapludoll and Typic Argiudoll). We focused on the changes of soil structural porosity in both methods (ShC and micromorphological analyses) in silty soils with low shrinkage-swelling capacity. The QP had a higher volume (or lower bulk density) in both soils. The slope of the structural phase was QP<ICL<CC, indicating lower structural stability, as a consequence of macropore destruction due to management intensity. Micromorphological analyses were in concordance to shrinkage analysis. QP showed higher structural pores from shrinkage analyses; and from micromorphometric analyses: higher Pores>50 um, a good pore orientation (vertical angles), and crumb microstructure derived from an intense biological activity were observed. In Typic Hapludoll, structural porosity in CC and ICL presented similar values according to ShC determinations. In Typic Argiudoll CC it was presented a lower values of structural porosity than in ICL. Similar results were estimated from micromorphological analyses (Pores>50 μm = QP(20.0%)>ICL(17.7%)>CC(16.0%)). CC and ICL were characterized by the development of platy peds and horizontally oriented planes, whereas ICL presented more biological activity. ShC and micromorphology analyses improved the understanding of soil functioning in these non-expansive soils, allowing the comparison between cattle trampling and continuous cropping in different soil types.

El proceso de compactación en general es descripto usando métodos que no distinguen tipos y tamaños de poros que son afectados. El ajuste de las curvas de contracción (CC) usando el modelo XP permite diferenciar los manejos de suelos con relación a la porosidad. Este método está en consonancia con las determinaciones micromorfológicas y micromorfométricas de cortes delgados. El objetivo de este trabajo fue examinar el potencial del análisis de las CC de los suelos para describir la degradación física de los mismos bajo distintos manejos de la región pampeana de la Argentina: agricultura continua (AC), rotación agrícola-ganadera (AG), respecto de una situación cuasi-prístina (CP), en dos suelos: Hapludol Típico y Argiudol Típico. Desde dos aproximaciones de análisis de CC y análisis micromorfológico en suelos limosos de baja capacidad de expansión/contracción. El tratamiento CP presentó el volumen más alto (menor densidad aparente) para los dos suelos. Las pendientes de la fase estructural presentaron el siguiente orden: CP<AG<AC, mayor valor más destrucción de macroporos. El análisis micromorfológico estuvo en concordancia con el análisis de contracción. CP presentó los mayores valores de porosidad estructural hallándose por análisis micromorfológico poros >50um, buena orientación, y una microestructura migajosa derivada de la intensa actividad biológica. En el Hapludol Típico, la porosidad estructural fue similar en AC y en AG. En el Argiudol Típico, AG presentó valores mayores de porosidad estructural respecto de AC. Similares tendencias fueron halladas en los cortes delgados (Poros>50 μm = CP(20.0%)>GA(17.7%)>AC(16.0%)). AC and AG se caracterizaron por el desarrollo de estructuras laminares con poros orientados horizontalmente, sin embargo, GA presentó una mayor actividad biológica. La complementación de las CC y el análisis micromorfológico mejoró el entendimiento del funcionamiento de suelos no expandibles, permitiendo la comparación entre manejos agrícolas continuos y aquellos con pisoteo animal en diferentes tipos de suelo.

ABSTRACT Introduction: Health-related loss of quality of life among dengue patients and their relatives, and unforeseen family expenses incurred for this reason, have not been sufficiently studied in Cuba. Objective: Describe the impact on quality of life, as well as the non-medical direct and indirect unforeseen expenses incurred by patients hospitalized with dengue and their relatives. Method: A cost analysis study was conducted based on data provided by patients hospitalized with dengue in Salvador Allende Clinical Surgical Hospital and their relatives in November 2013. A closed-ended questionnaire of 173 questions was applied in January-February 2014. Estimation was made of average health-related loss of quality of life, direct non-medical costs and indirect costs. Results: The 30-44 years age group was the most harshly affected during the episode. There was a predominance of the female sex (63.3%). Health-related quality of life decreased dramatically from an average 86.9 the day before the disease to 27.8 on the day when patients felt the worst. At the time of the interview, 51% of the patients perceived that they had not recovered from the disease. Mean hospital stay was 5 days. Unforeseen patient and family expenses rose to 61.24 CUC (CI 95%; 43.23-79.25 CUC), of which 66.2 % was spent on food and 13.6 % on transport. Average income loss was 25.30 CUC for patients and 4.50 CUC for relatives. Total average cost per patient was 89.25 CUC (CI 95% 67.33-110.29 CUC). Conclusions: Evidence was found of loss of quality of life as a result of dengue. Unforeseen expenses were high, representing three times the mean salary in the province of Havana.

RESUMEN Introducción: En Cuba no se han estudiado suficientemente los gastos de bolsillo y la pérdida de calidad de vida relacionada con la salud (de pacientes con dengue y sus familiares. Objetivo: Describir la afectación de la calidad de vida, los costos no médicos directos (gastos de bolsillo) e indirectos de pacientes hospitalizados con dengue y sus familiares. Método: Estudio de descripción de costos desde la perspectiva de pacientes y familiares, para los hospitalizados con dengue en el Clínico Quirúrgico ”Salvador Allende”, en noviembre de 2013. En enero-febrero de 2014 se aplicó un cuestionario cerrado de 173 preguntas. Se calcularon la pérdida promedio de calidad de vida relacionada con la salud, los costos no médicos directos y los costos indirectos. Resultados: Durante el episodio de dengue el grupo de edad más afectado fue el de 30-44 años. Predominaron las mujeres (63,3 %). La calidad de vida relacionada con la salud se afectó sensiblemente pasando como promedio de 86,9 el día antes de la enfermedad a 27,8 el día que las personas se sintieron más enfermas. En el momento de la entrevista, el 51 % de los pacientes percibían que no se habían recuperado de la enfermedad. Los pacientes estuvieron hospitalizados como promedio 5 días. Los gastos de bolsillo de pacientes y familiares ascendieron a 61,24 CUC (IC95% 43,23-79,25 CUC), en que el 66,2 % fue en alimentación y el 13,6 % en transporte. Las pérdidas promedio de ingreso fueron de 25,30 CUC para los pacientes y 4,50 CUC para los familiares. Los costos totales promedio por paciente fueron de 89,25 CUC (IC95% 67,33-110,29 CUC). Conclusiones: Se evidenció la pérdida de calidad de vida como consecuencia del dengue. Los gastos de bolsillo fueron altos y representan tres veces el valor del salario medio de la Provincia La Habana.

ABSTRACT: Justification There is a connection between smoking and substances use disorder, especially alcohol and cannabis. Nevertheless, health interventions seem not to be addressed to this approach Objective Evaluation of the motivation degree for the smoking quit after developing a brief psycho-educational intervention on smoking. Methodology It is a quantitative research of analytical type, almost experimental, developed by means of two experimental series, of the types pre- and post- intervention. A brief educational intervention is applied and there is an evaluation of the differences in the motivation for the smoking quit in a group before the intervention and in another one after the intervention Results There is a difference of 1,27 points in the degree of motivation found in the individuals regarding the experimental groups Conclusions Drug addict people are smokers with a high degree of nicotinic dependence, so it is necessary to design suitable strategies of quitting.

RESUMEN: Justificación Existe una asociación entre tabaquismo y TUS, especialmente con alcohol y cannabis. Sin embargo, las intervenciones sanitarias parecen no ir encaminadas a su enfoque. Objetivo Evaluar el grado de motivación para el abandono tabáquico tras realizar una intervención psicoeducativa breve sobre abordaje del tabaquismo. Metodología Se trata de una investigación cuantitativa de tipo analítico cuasiexperimental que se lleva a cabo mediante dos cohortes experimentales, del tipo pre- y post-intervención. Se aplica una intervención breve educativa y se evalúan las diferencias en la motivación para el abandono tabáquico en un grupo antes de la intervención y en otro grupo después de la misma Resultados Existe una diferencia de 1,27 puntos en el grado de motivación encontrado en los individuos respecto a los grupos experimentales Conclusiones Las personas drogodependientes son fumadoras con altos grados de dependencia nicotínica por lo que es preciso diseñar estrategias de cesación adecuadas.

Abstract Phenylketonuria (PKU, OMIM 261600) is predominantly caused by mutations in the PAH gene. One hundred and three Argentine PKU patients were studied by Sanger sequencing; 101 were completely characterized (90.3% were compound heterozygotes). Fifty-four different pathogenic variants were identified. Mutations were distributed all along the PAH gene but concentrated in exon 7 (26%), 12 (12%), 11 (10%), and 6 (10%). 77% were missense, and 77% affected the enzyme catalytic domain, nine mutations accounted for 57% of 179 studied alleles: p.Arg261Gln (Allele frequency(AF):10.6%), c.1066-11G>A (AF:9,5%), p.Arg408Trp (AF:8,3%), p.Tyr414Cys (AF:5,5%), p.Ala403Val, p.Val388Met, and p.Arg158Gln (AF: 5% each), p.Leu48Ser, and p.Ile65Thr (AF:4% each). The predicted phenotype was assigned by Guldberg´s arbitrary value (AV) and compared with the clinical phenotype based in tolerance to Phe intake. 29.1% (n:30) were hyperphenylalaninemias, 18.5% (n:19) mild-PKU, 27.2% (n:28) moderate-PKU and 25.2 % (n:26) classical-PKU. Genotype/phenotype correlation was statistically significant (p<0.001) Overall concordance was 62,5%: 93.3% in hyperphenylalaninemia, 64.7% in mild-PKU and 65.4% in classical patients. The moderate-PKU showed a weak concordance (17%) with milder AV prediction than clinical assessment. 74% of discordant moderate patients harbored p.Arg261Gln, and p.Val388Met. Our cohort is highly heterogeneous, with predominant Mediterranean influence (mainly Spanish), but with differences with other Latin-American countries.

RESUMO Este consenso de recomendações para a radioterapia (RT) hipofracionada de toda a mama foi organizado pela Sociedade Brasileira de Radioterapia (SBRT) considerando o cenário ideal para indicação e segurança na tecnologia aplicada. Questões de controvérsias e contraindicações (RT hipofracionada em pacientes submetidas à quimioterapia [QT], RT hipofracionada nas drenagens linfáticas, RT hipofracionada após mastectomia com ou sem reconstrução imediata, a realização de reforço de dose em leito cirúrgico [ou boost], RT hipofracionada em pacientes com idade menor que 50 anos, RT hipofracionada em mamas volumosas, RT hipofracionada em histologia de carcinoma in situ [CDIS]) foram discutidas em encontro presencial, sendo o consenso atingido quando existisse concordância de pelo menos 75% dos panelistas. O grau de recomendação foi também sugerido de acordo com o nível de evidência científico disponível, qualificado entre fraco, médio ou forte. Assim, este consenso deverá servir para auxiliar os especialistas da radioterapia brasileira em relação às indicações e particularidades dessa técnica, como uma alternativa segura e viável para a realidade nacional.

SUMMARY This recommendation consensus for hypofractionated whole-breast radiotherapy (RT) was organized by the Brazilian Society of Radiotherapy (SBRT) considering the optimal scenario for indication and safety in the technology applied. All controversies and contraindication matters (hypofractionated RT in patients who underwent chemotherapy [CT], hypofractionated RT in lymphatic drainage, hypofractionated RT after mastectomy with or without immediate reconstruction, boost during surgery, hypofractionated RT in patients under 50 years old, hypofractionated RT in large breasts, hypofractionated RT in histology of carcinoma in situ [DCIS]) was discussed during a meeting in person, and a consensus was reached when there was an agreement of at least 75% among panel members. The grade for recommendation was also suggested according to the level of scientific evidence available, qualified as weak, medium, or strong. Thus, this consensus will aid Brazilian radiotherapy experts regarding indications and particularities of this technique as a viable and safe alternative for the national reality.

Abstract Background: Chronic fluid overload is frequent in hemodialysis patients (P) and it associates with hypertension, left ventricular hypertrophy (LVH) and higher mortality. Moreover, echocardiographic data assessing fluid overload is limited. Our aim was to evaluate the relationship between fluid overload measured by bioimpedance spectroscopy (BIS) and different echocardiographic parameters. Methods: Cross-sectional observational study including 76 stable patients. Dry weight was clinically assessed. BIS and echocardiography were performed. Weekly time-averaged fluid overload (TAFO) and relative fluid overload (FO/ECW) were calculated using BIS measurements. Results: Based on TAFO three groups were defined: A- dehydrated, TAFO < -0.25 L 32 P (42%); B- normohydrated, TAFO between -0.25 and 1.5 l: 26 (34%); C- overhydrated, TAFO>1.5 l: 18 (24%). We found significant correlation between TAFO and left atrial volume index (LAVI) (r: 0.29; p = 0.013) but not with FO/ECW (r: 0.06; p = 0.61). TAFO, but not FO/ECW kept a significant relationship with LAVI (p = 0.03) using One-Way ANOVA test and linear regression methods. LVH was present in 73.7% (concentric 63.2%, eccentric in 10.5%). No differences between groups in the presence of LVH or left ventricular mass index were found. Conclusions: We found that left atrial volume index determined by echocardiographic Area-length method, but not left ventricle hypertrophy or dimensions of cavities, are related on hydration status based on bioimpedance measured time-averaged fluid overload (TAFO), and not with FO/ECW.

Resumen Introducción: La sobrehidratación es frecuente en pacientes en hemodiálisis (P) y se asocia con hipertensión, hipertrofia ventricular izquierda (LVH) y mayor mortalidad. Los datos ecocardiográficos evaluando sobrecarga hídrica son escasos. Nuestro objetivo fue evaluar la relación entre sobrehidratación medida por Bioimpedancia multifrecuencia (BIS) y parámetros ecocardiográficos. Métodos: Estudio transversal observacional, con 76 P estables; El peso seco fue determinado clínicamente; se realizaron ecocardiograma, BIS y analítica sanguínea. Se calcularon la sobrehidratación promedio semanal (TAFO) y sobrehidratación relativa (FO/ECW). Resultados: 3 grupos: A- deshidratados, TAFO < -0.25 L: 32 P (42,1%); B- normohidratado, TAFO -0.25 - 1.5 L: 26 P (34,2%); C- sobrehidratados TAFO > 1.5 L: 18 P (23,7%). Encontramos correlación significativa entre TAFO e índice de volumen auricular izquierdo (LVAI) (r: 0.29; p = 0.013) y no con FO/ECW (rho 0,06; p = 0,61). TAFO, pero no FO/ ECW, mantuvo una relación significativa con LVAI (p = 0,03) utilizando test de ANOVA y regresión lineal. LVH estuvo presente en 73,7% de P (concéntrica 63,2%, excéntrica 10,5%). No encontramos diferencias entre grupos en cuanto a la presencia de LVH, ni del índice de masa ventricular izquierda. Conclusiones: Nosotros observamos que el índice de volumen auricular izquierdo determinado por longitud de área medida por ecocardiograma y no la hipertrofia ventricular izquierda o dimensión de cavidades se relaciona con el estado de hidratación medido por sobrehidatación semanal y no con FO/ECW.

Objectives: To determine the prevalence of Campylobacter spp. in patient under age 11 years with acute diarrhea syndrome and determine microbial resistance to the drugs of choice for treatment in Paraguay, ciprofloxacin, erythromycin, and tetracycline. Materials and Methods: We conducted a retrospective, cross-sectional study with a non-probabilistic sample of 1110 consecutive cases and a confidence level of 95%. The population studied were pediatric patients under age 11 years whose fecal samples were forwarded by institutions belonging to the enteric pathogens network to the Laboratorio Central de Salud Pública (public health central laboratory) from 2010 to 2012. Results: Of the 1110 fecal samples Campylobacter spp. were isolated in 176 cases, for a prevalence of 16%. Resistance to quinolones was found in 49% of these, resistance to tetracycline in 28%, and resistance to macrolides (erythromycin) in 1%. Conclusion: A significant prevalence of Campylobacter spp. was found to be the etiological agent for acute diarrhea syndrome and including cultures for this pathogen in analysis of fecal samples should be routine. The resistance found to the drugs of choice for treatment implies a need for better attention to the issue of indiscriminate use of antibiotics.

Objetivos: Determinar la prevalencia de Campylobacter spp. en pacientes menores de 11 años con síndrome diarreico agudo e indagar la resistencia antimicrobiana con respecto a las drogas de elección para el tratamiento clínico con Ciprofloxacina, Eritromicina y Tetraciclina. Materiales y Métodos: Se realizó un estudio descriptivo, retrospectivo de corte transverso, muestreo no probabilístico de casos consecutivos, cuya muestra tiene un tamaño de 1110 con un nivel de confianza de 95%. La población estudiada fue de pacientes pediátricos menores de 11 años cuyas muestras de heces fueron remitidas por las instituciones integrantes de la Red de Enteropatógenos al Laboratorio Central de Salud Pública en el periodo 2010-2012. Resultados: De 1110 muestras de heces estudiadas se aislaron Campylobacter spp. en 176 de ellas y corresponde al 16% de prevalencia. Se observó resistencia a las quinolonas en un 49 % de las muestras estudiadas, así como una resistencia a Tetraciclina del 28% y 1% de resistencia a los macrólidos (Eritromicina). Conclusión: Se observó una prevalencia importante de Campylobacter spp. como agente etiológico de síndrome diarreico agudo y es relevante la incorporación del aislamiento de este patógeno como agente etiológico rutinario en los análisis de coprocultivos realizados. La resistencia observada a las drogas de elección utilizadas como tratamiento nos obliga a tener en cuenta el control del uso indiscriminado de antimicrobianos

Este estudo objetivou examinar os efeitos de um Treinamento da Memória de Trabalho (TMT) em idosos saudáveis. Vinte participantes compuseram a amostra final, onze do grupo experimental (TMT) e nove do controle (socialização). Todos foram submetidos a uma avaliação neuropsicológica pré e pós-intervenção. Os encontros foram realizados uma vez por semana, durante três meses. Houve melhora significativa, no grupo experimental, em atenção concentrada, aprendizagem, memória de curto prazo e episódica, e no grupo controle, em um menor número de variáveis, na atenção concentrada e memória episódica. O TMT parece ter promovido efeito de transferência, principalmente na memória episódica, que é relacionada diretamente aos subsistemas da memória de trabalho (MT), sugerindo que o TMT pode ser útil no contexto da neuropsicologia do envelhecimento.

The present study examined the effects of a Working Memory Training (WMT) in healthy elderly. Twenty participants comprised the final sample, eleven from the experimental group (WMT) and nine from the control group (socialization). Every subject underwent a neuropsychological evaluation pre and post-intervention. The meetings were held once a week, for three months. Results indicated that subjects in the experimental group improved cognitive functions related to concentrated attention, learning, short-term and episodic memory. Subjects in the control group also demonstrated, in a smaller number of variables, improvement in concentrated attention and episodic memory. WMT seems to have generated a transfer effect, especially to episodic memory, which is directly related to the subsystem of working memory (WM), suggesting that WMT may be useful in the context of the neuropsychology of aging.

In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance of these two methods was assessed using DNA extracted from bovine tissues previously inoculated with several bacterial suspensions. PCR was performed on the same tissues before and after the formalin-fixed, paraffin-embedding procedure (FFPE tissues). The 23S rDNA PCR detected all fresh and FFPE positive tissues while the 16S rDNA-based protocol detected primarily the positive fresh tissues. Both methods are specific for pathogenic L. interrogans. The 23S-based PCR method successfully detected Leptospira in four dubious cases of human leptospirosis from archival tissue specimens and one leptospirosis-positive canine specimen. A sensitive method for leptospirosis identification in FFPE tissues would be a useful tool to screen histological specimen archives and gain a better assessment of human leptospirosis prevalence, especially in tropical countries, where large outbreaks can occur following the rainy season.

Este trabalho teve por objetivo avaliar o desempenho de novilhos de corte em pastagem nativa (PN), suplementados com níveis de farelo de arroz integral (FAI) de 0; 0,5; 1,0 e 1,5% do peso vivo dos animais, num delineamento inteiramente casualizado em parcelas subdivididas, durante 126 dias. Na parcela principal, foram alocados os níveis de suplemento e, nas subparcelas, os períodos de tempo (6 períodos de 21 dias). A suplementação foi oferecida de forma individual, duas vezes por dia, às 7h e às 15h:30min. O método de pastejo empregado foi o contínuo com lotação fixa e carga variável. As médias encontradas para proteína bruta foram de 6,8 e 14,9%, e para os coeficientes de digestibilidade "in vitro" da matéria orgânica, foram de 59,54 e 71,47% na PN e no FAI, respectivamente (dados expressos na matéria seca). Os ganhos médios diários dos animais foram de 0,35; 0,55; 0,53; e 0,63kg animal-1 dia-1 para os níveis de inclusão de farelo de arroz de 0; 0,5; 1,0 e 1,5% do peso vivo dos animais, diferindo entre animais suplementados e não-suplementados. Os resultados de rendimento e peso de carcaça fria dos animais não diferiram entre os tratamentos. A suplementação com FAI nos níveis entre 0,5 a 1,5% proporcionou incremento no ganho médio diário de aproximadamente 60%, quando comparado com os animais que não receberam suplemento.

This study was aimed at investigating steers performance on native pasture supplemented with levels of full fat rice bran: 0; 0.5; 1.0 and 1.5% of body weight of the animals, in a completely randomized design, with a split plot arrangement, during 126 days. In main plots were allocated the levels of supplement and in a split plot, the periods of time (6 periods of 21 days each). The supplementation was individual, offered twice a day, at 7am and 3.3pm. The system of pasture was continuous grazing with fix stocking density and variable stocking rate. The average percent of crude protein were 6.8% and 14.9% and the in vitro digestibility coefficients were 59.54% and 71.47% in native pasture and rice bran, respectively (in the dry matter). The live weight gains of the animals were 0.35; 0.55; 0.53 and 0.63kg d-1 with the rice bran daily inclusion of 0.0; 0.5; 1.0 and 1.5% of the animals live weigh differing only when it was considered the use or not use of the supplement. The carcass yield and weigh do not differ among the treatments. The supplementation with full fat rice bran in the levels between 0.5% and 1.5% of live weight proportioned an increase on the live weigh gain of approximately 60% when compared with the animals that did not receive supplement.

Se presenta el caso de un recién nacido, cuyo nacimiento ocurrió en el Hospital «Iván Portuondo», en San Antonio de los Baños y en quien, inmediatamente después del nacimiento, se constata salida de contenido fecal a través de la zona umbilical. Se realiza el estudio contrastado con lipiodol y se comprueba el diagnóstico clínico y radiológico en la intervención quirúrgica. Se revisa la bibliografía, se muestran las fotografías al respecto y se expone el caso.

A study was carried out on T. cruzi infection in pregnant women in the Guemes area in Salta province; the rate of infection was 12.3%. Of newborn children studied by the microhematocrit technique, 8.8% were diagnosed with Chagas' disease. All of them were treated with benznidazol (5mg/kg/day) for one month; all of them presented anemia, which was interpreted to be an adverse drug reaction. The microhematocrit technique is believed to represent the best option for diagnosis of this disease in newborn children. A diagnostic methodology to follow the course of the infection is proposed.

Se estudió la infección por T. cruzi en mujeres embarazadas en la localidad de General Güemes, provincia de Salta. La misma fue del 12,3 %. El 8,8% de los recién nacidos estudiados tuvieron diagnóstico de Enfermedad de Chagas utilizando la técnica directa (microhematocrito). Todos fueron tratados con benznidazol a razón de 5mg/kg/día durante 30 dias. Todos presentaron anemia, que fue interpretada como reacción adversa medicamentosa. Se estima que la técnica directa representa la mejor opción para llevar a cabo el diagnóstico de esta enfermedad en el recién nacido. Se ha propuesto un flujograma para el seguimiento de la infección por T. cruzi en el recién nacido.

Physical examination of nails was carried out in 210 elderly patients and nail scrapings were obtained from onychomycosis suggested lesions in order to determine their causative agents, incidence and clinical characteristics. Diagnostic was confirmed by the isolation of the agents from 74 patients, mainly from toe-nails (incidence 35,2). Tinea pedis occurred in 25% of the cases and Diabetes mellitus was the most prevalent associated disease and the most frequent clinical characteristics were the thickening, the opacity and the presence of longitudinal strias in the surface of the nails. It was compared the results obtained by microscopic examination and by culture. Trichophyton ru-brum was the most common dermatophyte isolated; Candida parapsilosis was dominant among Candida species.

Se realizó examen físico de las uñas a 210 ancianos y a aquellos que presentaron lesiones sugestivas de onicomicosis se les realizó toma de muestra con el objetivo de conocer los principales agentes causales, las características e incidencia de las lesiones. Se confirmó el diagnóstico mediante el aislamiento del agente causal en 74 de los casos procedentes principalmente de las uñas de los pies, para una incidencia de 35,2. La tinea pedis se presentó en el 25,7% de los casos, mientras que la enfermedad asociada más frecuente fue la Diabetes mellitus; entre las características clínicas de las uñas predominaron el engrosamiento, la pérdida del brillo y la aparición de estrías longitudinales. Existió una correspondencia significativa entre el examen microscópico directo y el cultivo. La especie de dermatofito predominate fue Trichophyton rubrum, mientras que Candida parapsilosis fue la más aislada entre las especies de Candida.