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au:FIGUEIREDO, REGINA MARIA PINTO DE

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Elephant grass silage with addition of regional by-products
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Figueiredo, Mércia Regina Pereira de

; Teixeira, Alice Cristina Bittencourt

; Bittencourt, Luciene Lignani

; Moreira, Guilherme Rocha

; Ribeiro, Ariane Jesus

; Silva, Frank Sinatra Gomes da

; Santos, André Luiz Pinto dos

; Costa, Maria Lindomárcia Leonardo da

Acta Scientiarum. Animal Sciences

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2022, Volumen 44 elocation e56616

Resumen: En | Texto: En | PDF: En

ABSTRACT. This study examines the effect of adding coffee husks (CH), cacao by-product (CBP) and passion fruit by-product (PBP) (fresh-matter basis) in the silage of elephant grass cv. Napier on nutritional characteristics. The experiment was laid out in a completely randomized design with a 3 × 4 factorial arrangement represented by three additives (CH, CBP or PBP) and four inclusion levels (0, 12, 24 or 36%). Four replicates were used per treatment. The material was ensiled in experimental mini-silos that were opened 60 days later for chemical analysis of the produced silages. The dry matter content of the silages with CH and PBP included at levels greater than 25% was adequate. The silage with PBP inclusion showed the highest crude protein levels and the best results for in vitro dry matter digestibility. In the silage containing CH, the neutral detergent fiber content decreased linearly with increasing inclusion of the husks. The silage with CBP showed the lowest dry matter and crude protein levels and the highest pH. In conclusion, the inclusion of up to 36% PBP in the ensiling of elephant grass is recommended, as the resulting material has potential for use in ruminant feeding at times of forage scarcity.

https://doi.org/10.4025/actascianimsci.v44i1.56616

Mayaro virus detection in the western region of Pará state, Brazil
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Saatkamp, Cassiano Junior

; Rodrigues, Luís Reginaldo Ribeiro

; Pereira, Andrew Mairom Nogueira

; Coelho, João Alberto

; Marques, Rose Grace Brito

; Souza, Victor Costa de

; Nascimento, Valdinete Alves do

; Saatkamp, Jamille Gomes dos Santos

; Naveca, Felipe Gomes

; Figueiredo, Regina Maria Pinto de

Revista da Sociedade Brasileira de Medicina Tropical

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2021, Volumen 54 elocation e0055-2020

Resumen: En | Texto: En | PDF: En

Abstract INTRODUCTION Mayaro virus (MAYV) was found in Pará state, Brazil, in 1955. Since then, sporadic outbreaks have occurred in different regions of the country. METHODS: Serum sample were collected from 49 individuals in 2016 and were initially tested for dengue virus (DENV) by real-time (RT) polymerase chain reaction (PCR). DENV-negative samples were tested for MAYV and Oropouche virus (OROV) by multiplexed RT quantitative PCR. RESULTS: All samples were negative for DENV and OROV, but MAYV was detected in four samples. CONCLUSIONS: Differential diagnoses of acute febrile syndrome are required, especially in regions where several arboviruses with similar clinical manifestations are endemic.

https://doi.org/10.1590/0037-8682-0055-2020 393 descargas

Human parvovirus B19 genotype 1 in suspected dengue patients of Tefé, Amazonas State, Brazil
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Figueiredo, Regina Maria Pinto de

; Souza, Victor Costa de

; Nascimento, Valdinete Alves do

; Naveca, Felipe Gomes

Revista da Sociedade Brasileira de Medicina Tropical

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2019, Volumen 52 elocation e20190304

Resumen: En | Texto: En | PDF: En

Abstract INTRODUCTION: Human parvovirus B19 (B19V) is a common pathogen, which on infection causes variety of clinical conditions from benign self-limiting exanthematous disease and other similar pathologies to fetal death. METHODS: We collected 341 serum samples between the first and fourth day after the onset of symptoms from all patients suspected of dengue fever who were attended at Regional Hospital of Tefé. Initially, patients were screened for malaria by blood smear test and negative samples were sent to Fundação de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD) situated in Manaus (AM) for dengue testing using semi-nested multiplex PCR. Further, we investigated 44 malaria and dengue-negative samples of children for B19V DNA by nested-PCR. Positive samples were analyzed by BLAST against entire public non-redundant nucleotide database and genotyped by phylogenetic analyses using neighbor-joining clustering method. RESULTS: Eight samples (18.2%) were found to be PCR positive. Fever, headache, ocular pain, and/or muscle pain were reported as the most frequent symptoms by the patients and none were diagnosed with rash at the time of sample collection. Phylogenetic analysis of major capsid protein 2 (VP2) and VP3 coding region showed high similarity with B19V genotype 1. CONCLUSIONS: Our results reveal the spread of B19V genotype 1 in Tefé. Moreover, our results emphasize the significance of laboratorial differential diagnosis using molecular techniques in patients with acute febrile, and thereby aid the health surveillance system in improving patient care even in the remote areas of Amazon.

https://doi.org/10.1590/0037-8682-0304-2019 1322 descargas

Recommendations of the Brazilian Society of Rheumatology for the diagnosis and treatment of chikungunya fever. Part 2 - Treatment
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Marques, Claudia Diniz Lopes

; Duarte, Angela Luzia Branco Pinto

; Ranzolin, Aline

; Dantas, Andrea Tavares

; Cavalcanti, Nara Gualberto

; Gonçalves, Rafaela Silva Guimarães

; Rocha Junior, Laurindo Ferreira da

; Valadares, Lilian David de Azevedo

; Melo, Ana Karla Guedes de

; Freire, Eutilia Andrade Medeiros

; Teixeira, Roberto

; Neto, Francisco Alves Bezerra

; Medeiros, Marta Maria das Chagas

; Carvalho, Jozélio Freire de

; Santos, Mario Sergio F.

; Océa, Regina Adalva de L. Couto

; Levy, Roger A.

; Andrade, Carlos Augusto Ferreira de

; Pinheiro, Geraldo da Rocha Castelar

; Abreu, Mirhelen Mendes

; Verztman, José Fernando

; Merenlender, Selma

; Ribeiro, Sandra Lucia Euzebio

; Costa, Izaias Pereira da

; Pileggi, Gecilmara

; Trevisani, Virginia Fernandes Moça

; Lopes, Max Igor Banks

; Brito, Carlos

; Figueiredo, Eduardo

; Queiroga, Fabio

; Feitosa, Tiago

; Tenório, Angélica da Silva

; Siqueira, Gisela Rocha de

; Paiva, Renata

; Vasconcelos, José Tupinambá Sousa

; Christopoulos, Georges

Revista Brasileira de Reumatologia

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2017, Volumen 57 Supl. 2 Paginas s438 - s451

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo A febre chikungunya tem se tornado um importante problema de saúde pública nos países onde ocorrem as epidemias, visto que metade dos casos evolui com artrite crônica, persistente e incapacitante. Os dados na literatura sobre terapêuticas específicas nas diversas fases da artropatia ocasionada pela infecção pelo vírus chikungunya (CHIKV) são limitados, não existem estudos randomizados de qualidade que avaliem a eficácia das diferentes terapias. Há algumas poucas publicações sobre o tratamento das manifestações musculoesqueléticas da febre chikungunya, porém com importantes limitações metodológicas. Os dados atualmente disponíveis não permitem conclusões favoráveis ou contrárias a terapêuticas específicas, bem como uma adequada avaliação quanto à superioridade entre as diferentes medicações empregadas. O objetivo deste trabalho foi elaborar recomendações para o tratamento da febre chikungunya no Brasil. Foi feita uma revisão da literatura com seleção de artigos baseados em evidência, nas bases de dados Medline, SciELO, PubMed e Embase e de resumos de anais de congressos, além da opinião dos especialistas para dar apoio às decisões tomadas para definir as recomendações. Para a definição do grau de concordância foi feita uma metodologia Delphi, em duas reuniões presenciais e várias rodadas de votação on line. Este artigo refere-se à parte 2 das Recomendações da Sociedade Brasileira de Reumatologia para Diagnóstico e Tratamento da Febre Chikungunya, que trata especificamente do tratamento.

Abstract Chikungunya fever has become an important public health problem in countries where epidemics occur because half of the cases progress to chronic, persistent and debilitating arthritis. Literature data on specific therapies at the various phases of arthropathy caused by chikungunya virus (CHIKV) infection are limited, lacking quality randomized trials assessing the efficacies of different therapies. There are a few studies on the treatment of musculoskeletal manifestations of chikungunya fever, but these studies have important methodological limitations. The data currently available preclude conclusions favorable or contrary to specific therapies, or an adequate comparison between the different drugs used. The objective of this study was to develop recommendations for the treatment of chikungunya fever in Brazil. A literature review was performed via evidence-based selection of articles in the databases Medline, SciELO, PubMed and Embase and conference proceedings abstracts, in addition to expert opinions to support decision-making in defining recommendations. The Delphi method was used to define the degrees of agreement in 2 face-to-face meetings and several online voting rounds. This study is part 2 of the Recommendations of the Brazilian Society of Rheumatology (Sociedade Brasileira de Reumatologia - SBR) for the Diagnosis and Treatment of chikungunya fever and specifically addresses treatment.

https://doi.org/10.1016/j.rbre.2017.06.004 38509 descargas

Recommendations of the Brazilian Society of Rheumatology for diagnosis and treatment of Chikungunya fever. Part 1 - Diagnosis and special situations
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Marques, Claudia Diniz Lopes

; Duarte, Angela Luzia Branco Pinto

; Ranzolin, Aline

; Dantas, Andrea Tavares

; Cavalcanti, Nara Gualberto

; Gonçalves, Rafaela Silva Guimarães

; Rocha Junior, Laurindo Ferreira da

; Valadares, Lilian David de Azevedo

; Melo, Ana Karla Guedes de

; Freire, Eutilia Andrade Medeiros

; Teixeira, Roberto

; Bezerra Neto, Francisco Alves

; Medeiros, Marta Maria das Chagas

; Carvalho, Jozélio Freire de

; Santos, Mario Sergio F.

; Océa, Regina Adalva de L. Couto

; Levy, Roger A.

; Andrade, Carlos Augusto Ferreira de

; Pinheiro, Geraldo da Rocha Castelar

; Abreu, Mirhelen Mendes

; Verztman, José Fernando

; Merenlender, Selma

; Ribeiro, Sandra Lucia Euzebio

; Costa, Izaias Pereira da

; Pileggi, Gecilmara

; Trevisani, Virginia Fernandes Moça

; Lopes, Max Igor Banks

; Brito, Carlos

; Figueiredo, Eduardo

; Queiroga, Fabio

; Feitosa, Tiago

; Tenório, Angélica da Silva

; Siqueira, Gisela Rocha de

; Paiva, Renata

; Vasconcelos, José Tupinambá Sousa

; Christopoulos, Georges

Revista Brasileira de Reumatologia

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2017, Volumen 57 Supl. 2 Paginas s421 - s437

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo A febre chikungunya tem se tornado um importante problema de saúde pública nos países onde ocorrem as epidemias. Até 2013, as Américas haviam registrado apenas casos importados quando, em outubro desse mesmo ano, foram notificados os primeiros casos na Ilha de Saint Martin, no Caribe. No Brasil, os primeiros relatos autóctones foram confirmados em setembro de 2014 e até a semana epidemiológica 37 de 2016 já haviam sido registrados 236.287 casos prováveis de infecção pelo chikungunya vírus (CHIKV), 116.523 confirmados sorologicamente. As mudanças ambientais causadas pelo homem, o crescimento urbano desordenado e o número cada vez maior de viagens internacionais têm sido apontados como os fatores responsáveis pela reemergência de epidemias em grande escala. Caracterizada clinicamente por febre e dor articular na fase aguda, em cerca de metade dos casos existe evolução para a fase crônica (além de três meses), com dor persistente e incapacitante. O objetivo deste trabalho foi elaborar recomendações para diagnóstico e tratamento da febre chikungunya no Brasil. Para isso, foi feita revisão da literatura nas bases de dados Medline, SciELO e PubMed, para dar apoio às decisões tomadas para definir as recomendações. Para a definição do grau de concordância foi feita uma metodologia Delphi, em duas reuniões presenciais e várias rodadas de votação on line. Foram geradas 25 recomendações, divididas em três grupos temáticos: (1) diagnóstico clínico, laboratorial e por imagem; (2) situações especiais e (3) tratamento. Na primeira parte estão os dois primeiros temas e o tratamento na segunda.

Abstract Chikungunya fever has become a relevant public health problem in countries where epidemics occur. Until 2013, only imported cases occurred in the Americas, but in October of that year, the first cases were reported in Saint Marin island in the Caribbean. The first autochthonous cases were confirmed in Brazil in September 2014; until epidemiological week 37 of 2016, 236,287 probable cases of infection with Chikungunya virus had been registered, 116,523 of which had serological confirmation. Environmental changes caused by humans, disorderly urban growth and an ever-increasing number of international travelers were described as the factors responsible for the emergence of large-scale epidemics. Clinically characterized by fever and joint pain in the acute stage, approximately half of patients progress to the chronic stage (beyond 3 months), which is accompanied by persistent and disabling pain. The aim of the present study was to formulate recommendations for the diagnosis and treatment of Chikungunya fever in Brazil. A literature review was performed in the MEDLINE, SciELO and PubMed databases to ground the decisions for recommendations. The degree of concordance among experts was established through the Delphi method, involving 2 in-person meetings and several online voting rounds. In total, 25 recommendations were formulated and divided into 3 thematic groups: (1) clinical, laboratory and imaging diagnosis; (2) special situations; and (3) treatment. The first 2 themes are presented in part 1, and treatment is presented in part 2.

https://doi.org/10.1016/j.rbre.2017.05.006 37996 descargas

Arboviral diseases in the Western Brazilian Amazon: a perspective and analysis from a tertiary health & research center in Manaus, State of Amazonas
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Mourão, Maria Paula Gomes

; Bastos, Michele de Souza

; Figueiredo, Regina Maria Pinto de

; Gimaque, João Bosco de Lima

; Alves, Valquíria do Carmo Rodrigues

; Saraiva, Maria das Graças Gomes

; Figueiredo, Mário Luis Garcia

; Ramasawmy, Rajendranath

; Nogueira, Maurício Lacerda

; Figueiredo, Luiz Tadeu Moraes

Revista da Sociedade Brasileira de Medicina Tropical

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Jun 2015, Volumen 48 Supl. 1 Paginas 20 - 26

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The Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD), located in Manaus, the capital of the State of Amazonas (Western Brazilian Amazon), is a pioneering institution in this region regarding the syndromic surveillance of acute febrile illness, including arboviral infections. Based on the data from patients at the FMT-HVD, we have detected recurrent outbreaks in Manaus by the four dengue serotypes in the past 15 years, with increasing severity of the disease. This endemicity has culminated in the simultaneous circulation of all four serotypes in 2011, the first time this has been reported in Brazil. Between 1996 and 2009, 42 cases of yellow fever (YF) were registered in the State of Amazonas, and 71.4% (30/42) were fatal. Since 2010, no cases have been reported. Because the introduction of the yellow fever virus into a large city such as Manaus, which is widely infested by Aedes mosquitoes, may pose a real risk of a yellow fever outbreak, efforts to maintain an appropriate immunization policy for the populace are critical. Manaus has also suffered silent outbreaks of Mayaro and Oropouche fevers lately, most of which were misdiagnosed as dengue fever. The tropical conditions of the State of Amazonas favor the existence of other arboviruses capable of producing human disease. Under this real threat, represented by at least 4 arboviruses producing human infections in Manaus and in other neighboring countries, it is important to develop an efficient public health surveillance strategy, including laboratories that are able to make proper diagnoses of arboviruses.

https://doi.org/10.1590/0037-8682-0133-2013 6541 descargas

Identification of dengue viruses in naturally infected Aedes aegypti females captured with BioGents (BG)-Sentinel traps in Manaus, Amazonas, Brazil
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Figueiredo, Regina Maria Pinto de

; Mourão, Maria Paula Gomes

; Abi-Abib, Yasmin Emile Conte

; Oliveira, Cintia Mara de

; Roque, Rosemary

; Azara, Tatiana de

; Ohly, Jorg

; Degener, Carolin

; Geier, Martin

; Eiras, Álvaro Eduardo

Revista da Sociedade Brasileira de Medicina Tropical

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Abr 2013, Volumen 46 Nº 2 Paginas 221 - 222

Resumen: En | Texto: En | PDF: En

IntroductionIn Manaus, the first autochthonous cases of dengue fever were registered in 1998. Since then, dengue cases were diagnosed by the isolation of viruses 1, 2, 3, and 4.MethodsOne hundred eighty-seven mosquitoes were collected with BioGents (BG)-Sentinel traps in 15 urban residential areas in the Northern Zone of Manaus and processed by molecular tests.ResultsInfections with dengue viruses 1, 2, 3, and 4 and a case of co-infection with dengue viruses 2 and 3 were identified.ConclusionsThese findings corroborate the detection of dengue in clinical samples and reinforce the need for epidemiological surveillance by the Health authorities.

https://doi.org/10.1590/0037-8682-1096-2013 3278 descargas

Simultaneous circulation of all four dengue serotypes in Manaus, State of Amazonas, Brazil in 2011
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Bastos, Michele de Souza

; Figueiredo, Regina Maria Pinto de

; Ramasawmy, Rajendranath

; Itapirema, Evaulino

; Gimaque, João Bosco Lima

; Santos, Lucilaide Oliveira

; Figueiredo, Luiz Tadeu Moraes

; Mourão, Maria Paula Gomes

Revista da Sociedade Brasileira de Medicina Tropical

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Jun 2012, Volumen 45 Nº 3 Paginas 393 - 394

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

INTRODUÇÃO: Manaus, capital do Estado do Amazonas, com quase 2 milhões de habitantes, está localizada no meio da floresta Amazônica e vem sofrendo surtos de dengue desde 1998. MÉTODOS: Neste estudo, amostras de sangue foram investigadas pela Transcriptase reversa-reação em cadeia da polimerase (RT-PCR), visando identificar os sorotipos de vírus da dengue. RESULTADOS: Soros de fase aguda de 432 pacientes foram testados para a presença do vírus da dengue. Destes, 137 (31,3%) foram considerados positivos. Todos os quatro sorotipos do vírus da dengue foram observados. CONCLUSÕES: A circulação simultânea dos quatro sorotipos da dengue é descrita pela primeira vez em Manaus e no Brasil.

INTRODUCTION: Manaus, the capital city of the state of Amazon with nearly 2 million inhabitants, is located in the middle of the Amazon rain forest and has suffered dengue outbreaks since 1998. METHODS: In this study, blood samples were investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), aimed at identifying dengue virus serotypes. RESULTS: Acute phase sera from 432 patients were tested for the presence of dengue virus. Out of the 432 patients, 137 (31.3%) were found to be positive. All the four dengue virus serotypes were observed. CONCLUSIONS: The simultaneous circulation of the four dengue serotypes is described for the first time in Manaus and in Brazil.

4548 descargas

Serological evidence of hantavirus infection in rural and urban regions in the state of Amazonas, Brazil
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Gimaque, João Bosco Lima

; Bastos, Michele de Souza

; Braga, Wornei Silva Miranda

; Oliveira, Cintia Mara Costa de

; Castilho, Márcia da Costa

; Figueiredo, Regina Maria Pinto de

; Galusso, Elizabeth dos Santos

; Itapirema, Evaulino Ferreira

; Figueiredo, Luiz Tadeu Moraes de

; Mourão, Maria Paula Gomes

Memórias do Instituto Oswaldo Cruz

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Feb 2012, Volumen 107 Nº 1 Paginas 135 - 137

Resumen: En | Texto: En | PDF: En

Hantavirus disease is caused by the hantavirus, which is an RNA virus belonging to the family Bunyaviridae. Hantavirus disease is an anthropozoonotic infection transmitted through the inhalation of aerosols from the excreta of hantavirus-infected rodents. In the county of Itacoatiara in the state of Amazonas (AM), Brazil, the first human cases of hantavirus pulmonary and cardiovascular syndrome were described in July 2004. These first cases were followed by two fatal cases, one in the municipality of Maués in 2005 and another in Itacoatiara in 2007. In this study, we investigated the antibody levels to hantavirus in a population of 1,731 individuals from four different counties of AM. Sera were tested by IgG/IgM- enzyme-linked immune-sorbent assay using a recombinant nucleocapsid protein of the Araraquara hantavirus as an antigen. Ten sera were IgG positive to hantavirus (0.6%). Among the positive sera, 0.8% (1/122), 0.4% (1/256), 0.2% (1/556) and 0.9% (7/797) were from Atalaia do Norte, Careiro Castanho, Itacoatiara and Lábrea, respectively. None of the sera in this survey were IgM-positive. Because these counties are distributed in different areas of AM, we can assume that infected individuals are found throughout the entire state, which suggests that hantavirus disease could be a local emerging health problem.

3012 descargas

10.

Presença de parvovírus B19 em Manaus, AM
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Figueiredo, Regina Maria Pinto de

; Lima, Mário Lira de

; Almeida, Tânia Maria Carvalho

; Bastos, Michele de Souza

Revista da Sociedade Brasileira de Medicina Tropical

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Oct 2005, Volumen 38 Nº 5 Paginas 396 - 398

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

A investigação de 1.107 casos de doenças exantemáticas em Manaus permitiu a identificação dos primeiros 47 casos de parvovírus humano B19 na cidade. O parvovírus B19 foi caracterizado por uma combinação de sinais e sintomas como febre, cefaléia, artralgia, mialgia e exantema. A freqüência de exantema foi maior em indivíduos menores de quinze anos e, no adulto, prevaleceram a febre e artropatias. O maior número de casos foi registrado em 1999. Quanto à faixa etária, nos menores de 15 anos, predominou o sexo masculino e, entre os adultos, o feminino. Este estudo, portanto, ressalta a necessidade de se elucidar a causa de doenças exantemáticas que ocorrem no Estado do Amazonas e indica que estudos são necessários, no que concerne à atividade viral.

Investigation of 1,107 cases of exanthematous diseases in Manaus allowed the identification of the first 47 cases of human parvovirus B19 in the city. Parvovirus B19 was characterized by a combination of signs and symptoms such as fever, chronic headache, arthralgia, myalgia and exanthema. The frequency of exanthema was higher in individuals less than fifteen years old. In adults, fever and arthropathy were the most frequent symptoms. The highest number of cases was registered in 1999. Regarding gender, male sex was predominant among infected individuals younger than fifteen years old, and female sex in adults. This study, therefore, underscores the necessity for clarifying the causes of exanthematous illnesses that occur in the State of Amazonas and investigation of the viral activity.

4563 descargas

11.

Doenças exantemáticas e primeira epidemia de dengue ocorrida em Manaus, Amazonas, no período de 1998-1999
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Figueiredo, Regina Maria Pinto De

; Thatcher, Bedsy Dutary

; Lima, Mário Lira de

; Almeida, Tânia Carvalho

; Alecrim, Wilson Duarte

; Guerra, Marcus Vinitius de Farias

Revista da Sociedade Brasileira de Medicina Tropical

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Dic 2004, Volumen 37 Nº 6 Paginas 476 - 479

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Em 1998, a Fundação de Medicina Tropical/Instituto de Medicina Tropical do Amazonas implementou o sistema de vigilância para síndromes febris agudas indiferenciadas, com o propósito de manter vigilância ativa e passiva na Amazônia Ocidental, Brasil, permitindo identificar e diagnosticar os agentes etiológicos causadores de febres agudas. O diagnóstico foi realizado através de estudos sorológicos para a detecção de anticorpos IgM, utilizando-se técnicas de ELISA (Enzyme-linked-immunosorbent assay) e kits ELISA comerciais. Foram analisadas 8.557 amostras de soros de pacientes com suspeita clínica de dengue, 40% dos soros foram ELISA positivos para o vírus da dengue e 26% dos soros foram ELISA positivos para outras doenças exantemáticas virais como rubéola, sarampo, parvovírus, oropouche e mayaro.

In 1998, the FMT/IMT-AM foundation implemented the surveillance system to diagnose acute undifferentiated febrile syndromes, with the objective of active and passive surveillance in Brazilian western Amazonian rainforest to identify and diagnose the etiologic agents of acute fever. The diagnoses were performed using serological tests to detect IgM antibodies by ELISA (Enzyme-linked-immunosorbent assay) CDC/OPAS or using commercial kits. A total of 8,557 serum samples obtained from patients with clinical suspicion of dengue virus were analyzed. ELISA positive reaction to dengue virus was presented by 40% of the serum samples and 26% of the serum samples had positive ELISA reactions to other exanthematous viral diseases, such as rubella, measles, parvovirus, Oropouche virus and Mayaro virus.

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