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Abstract: The aim of this study was to use microscopic and molecular techniques to evaluate the effects of a single session of antimicrobial photodynamic therapy (aPDT) on the alveolar repair process after tooth extraction in rats. The study sample included 84 rats divided into four groups, as follows: a) Control - untreated socket; b) Laser - socket treated using photobiomodulation; c) TBO - socket treated with topic application of the photosensitizer agent, toluidine blue O (TBO); and d) aPDT - socket treated with TBO and laser irradiation. An additional rat was used for thermal mapping during socket irradiation. The animals were euthanatized at 6, 15, and 28 days after unilateral extraction of the upper incisor. Quantitative and qualitative analyses of the connective and bone tissues, blood clot, blood vessel, and inflammatory infiltrate were performed, and real-time polymerase chain reaction was used to study the expression of genes (collagen type I, osteocalcin, alkaline phosphatase [ALP], runt-related transcription factor 2 [RUNX2], and vascular endothelial growth factor [VEGF]) involved in the bone healing process. No statistically significant differences in microscopic and molecular outcomes were observed between the groups (p > 0.05). A positive correlation was seen to exist between blood clot and VEGF (p = 0.000), and a negative correlation was observed between bone tissue and ALP (p = 0.028) and blood vessel and VEGF (p = 0.018). A single session of aPDT in the dental extraction site did not influence the alveolar repair process in rats.

https://doi.org/10.1590/1807-3107bor-2022.vol36.0024

Snakebites caused by the genera Bothrops and Lachesis in the Brazilian Amazon: a study of factors associated with severe cases and death
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Magalhães, Samara Freire Valente

; Peixoto, Henry Maia

; Freitas, Lúcia Rolim Santana de

; Monteiro, Wuelton Marcelo

; Oliveira, Maria Regina Fernandes de

Revista da Sociedade Brasileira de Medicina Tropical

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2022, Volumen 55 elocation e0558-2021

Resumen: En | Texto: En | PDF: En

ABSTRACT Background: Snakebites are a major problem in developing countries owing to their high morbidity rates, severity, and sequelae. In Brazil, most cases of envenomation are caused by Bothrops and Lachesis snakes. The present study aimed to evaluate variables associated with death, systemic complications, and amputations in victims of envenomation due to Bothrops or Lachesis snake. Methods: An analytical epidemiological study was performed with data from the Notifiable Diseases Information System (Sistema de Informação de Agravos de Notificação [SINAN]) relating to the Brazilian Amazon for the period 2010-2015. A hierarchical Poisson regression analysis was performed with three aspects, namely, individual characteristics, sociodemographic characteristics, and clinical conditions. Results: The following associations were observed: i) advanced age (>65 years), sepsis, renal failure, and hemorrhagic manifestations were related to two outcomes - death due to snakebite and death from all causes; ii) more advanced age (≥46 years), time to health care longer than 6 hours, renal and hemorrhagic manifestations, and region of occurrence being rural areas were associated with systemic complications; and iii) children (up to 12 years old) were associated with amputations. Conclusions: Knowledge of the characteristics associated with severe outcomes in snakebites may help identify patients who will require more intensive care or longer follow-up and may provide the physician with counseling rationale for their possible prognosis.

https://doi.org/10.1590/0037-8682-0558-2021

Absence of Trichinella spp. larvae in carcasses of road-killed wild animals in Paraná state, Brazil
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Jurkevicz, Rafaela Maria Boson

; Silva, Douglas Aparecido da

; Ferreira Neto, José Maurício

; Matos, Andressa Maria Rorato Nascimento de

; Pires, Bárbara Giglio

; Paschoal, Aline Ticiani Pereira

; Pinto-Ferreira, Fernanda

; Bracarense, Ana Paula Frederico Loureiro

; Mitsuka-Breganó, Regina

; Freire, Roberta Lemos

; Navarro, Italmar Teodorico

; Caldart, Eloiza Teles

Revista Brasileira de Parasitologia Veterinária

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2022, Volumen 31 Nº 4 elocation e010622

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Trichinella spp. é um parasito zoonótico de distribuição mundial detectado em carnívoros e onívoros de sangue quente e humanos. Até o momento, o Brasil é considerado pela Organização Mundial de Saúde Animal livre do ciclo doméstico da doença, enquanto o ciclo silvestre do parasito tem status de infecção em áreas limitadas. No entanto, em países vizinhos como a Argentina, larvas do parasito foram encontradas na fauna silvestre. O presente estudo teve como objetivo determinar a ocorrência de Trichinella spp. em animais silvestres atropelados no Paraná, Brasil. Amostras biológicas de 71 animais silvestres – 29 Didelphis albiventris, 11 Nasua-nasua, dez Cerdocyon thous, sete Dasypus novemcinctus, seis Leopardus gattulus, seis Sphiggurus spinosus e dois Puma concolor– coletadas de novembro de 2016 a novembro de 2021 foram submetidas à digestão artificial, seguindo a metodologia descrita do REGULAMENTO (CE) Nº2075/2005. Não foram encontradas larvas de Trichinella spp. nas carcaças dos animais selvagens atropelados. No entanto, considerando o amplo espectro de possíveis reservatórios que podem atuar como elo entre os ciclos silvestre e doméstico e considerando a condição atual da triquinelose silvestre no Brasil em áreas limitadas, o monitoramento frequente da fauna silvestre é necessário.

Abstract Trichinella spp. are zoonotic parasites that are widely distributed in warm-blooded carnivores and omnivores, including humans. Until the present moment, Brazil has been considered by World Animal Health Organization free from the domestic cycle of trichinellosis, whereas the parasite’s sylvatic cycle has the status of infection in limited zones. However, neighboring countries such as Argentina have reports of parasite larvae in the wild fauna. The present study aimed to determine the occurrence of Trichinella spp. in road-killed wild animals in Paraná, Brazil. Biological samples from 71 wild animals—29 Didelphis albiventris, 11 Nasua nasua, ten Cerdocyon thous, seven Dasypus novemcinctus, six Leopardus guttulus, six Sphiggurus spinosus and two Puma concolor—collected from November 2016 to November 2021 were subjected to artificial digestion, following the methodology described in the REGULATION (EC) No. 2075/2005. No Trichinella spp. larvae were detected in the carcasses of the road-killed wild animals. However, considering the wide spectrum of possible reservoirs that could act as a link between the sylvatic and domestic cycles and considering the current Brazilian status of sylvatic trichinellosis in limited zones, frequent monitoring of wild fauna remains necessary.

https://doi.org/10.1590/s1984-29612022054

The REBECGA Brazilian Registry of Pregnancy and Heart Disease: Rationale and Design
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Avila, Walkiria Samuel

; Rivera, Maria Alayde Mendonça

; Marques-Santos, Celi

; Rivera, Ivan Romero

; Costa, Maria Elizabeth Navegantes Caetano

; Lucena, Alexandre Jorge Gomes de

; Freire, Claudia Maria Vilas

; Carvalho, Regina Coeli Marques de

; Born, Daniel

; Campanharo, Felipe Favorette

; Silva, Fabio Bruno

International Journal of Cardiovascular Sciences

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Ago 2021, Volumen 34 Nº 4 Paginas 452 - 458

Resumen: En | Texto: En | PDF: En

Abstract Background: Maternal mortality rates in Brazil remain above the goals established by the United Nations Sustainable Development Goals. Heart disease is estimated to affect 4% of all pregnancies and remains by itself the main indirect obstetric cause of maternal death. In the last decades, a significant improvement in the prognosis of heart diseases has made pregnancy possible in women with heart disease and provided better maternal and fetal outcomes. Objective: To establish a multicenter Brazilian Registry of pregnant women with heart disease; to study the causes of immediate and late maternal mortality; and to assess the prevalence of heart disease in the country’s macro-regions. Method: This is an observational study, with retrospective and prospective stages, of the clinical and obstetric progression of pregnant women with heart disease. These women consecutively received care during pregnancy and will be followed up for up to a year after delivery at public and private hospitals with infrastructure for the execution of this project, a principal investigator, and approval by Ethics and Research Committees. Results: Our results will be presented after data collection and statistical analysis, aiming to demonstrate immediate and late maternal mortality rates, as well as the prevalence of heart disease in the country and its cardiovascular and obstetric complications during pregnancy. Conclusion: REBECGA will be the Brazilian Registry of heart disease and pregnancy and it will contribute to planning preventive measures, raising financial resources for the improvement of high-risk prenatal care, and reducing immediate and late maternal mortality due to heart disease.

https://doi.org/10.36660/ijcs.20200419

Anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep from Paraná state, South Brazil: prevalence and associated factors
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Romanelli, Paulo Roberto

; Matos, Andressa Maria Rorato Nascimento de

; Pinto-Ferreira, Fernanda

; Caldart, Eloiza Teles

; Carmo, João Lucas Moura do

; Santos, Nathalia Gomes dos

; Silva, Natalia Ribeiro da

; Loeffler, Barbara Barcellos

; Sanches, João Fernando Zamariola

; Francisquini, Luiza Schumacher

; Romanelli, Micheline Sahyun

; Minho, Alessandro Pelegrine

; Cavalcante, Antônio Cezar Rocha

; Pierre, Elzira Jorge

; Sobezak, Claudio Cesar

; Freire, Roberta Lemos

; Mitsuka-Breganó, Regina

; Navarro, Italmar Teodorico

Revista Brasileira de Parasitologia Veterinária

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2021, Volumen 30 Nº 1 elocation e023220

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo O objetivo deste estudo foi avaliar a soroprevalência e os fatores de risco associados à presença de anticorpos contra Toxoplasma gondii e Neospora caninum em ovinos do Paraná. A detecção dos anticorpos para T. gondii e N. caninum foi realizada, usando-se testes de ELISA indireto caseiro e comercial, respectivamente. A análise de regressão logística múltipla foi usada para verificar os fatores de risco associados à soroprevalência. Anticorpos anti-T. gondii e anti-N. caninum foram observados em 42,7% e em 17,6% dos animais, respectivamente. Os fatores de proteção associados à soropositividade foram “algum nível de confinamento (confinamento ou semiextensivo)” (OR=0,53) para T. gondii e “presença de mão de obra especializada” para N. caminum (OR=0,64). Os fatores de risco foram “presença de gatos” para T. gondii (OR=1,75) e “alimentação de cães com resíduos placentários” (OR=1,79) para N. caninum. Além de apresentar uma significante e simultânea soroprevalência para ambos os agentes (9,9% dos animais), os resultados também indicam que as deficiências em gestão e saneamento ambiental, a presença de reservatórios e os tipos de exploração favorecem a soropositividade. Dessa forma, estudos como estes podem auxiliar em programas sanitários e políticas públicas para a prevenção de T. gondii e N. caninum nos rebanhos ovinos do Paraná.

Abstract The aim of this study was to evaluate the seroprevalence and factors associated with the presence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep from Paraná state. The detection of antibodies for T. gondii and N. caninum was performed by homemade and commercial indirect ELISA, respectively. Multiple logistic regression analysis was used to verify the factors associated with the seroprevalence. Antibodies anti-T. gondii and anti-N. caninum were observed in 42.7% and in 17.6% of the animals, respectively. The protective factors associated to seropositive were “some level of confinement” (full or semi-extensive confinement) (OR=0.53) for T. gondii and “use of skilled labor” (OR=0.64) for N. caninum. The risk factors were “presence of cats” (OR=1.75) for T. gondii and “feeding of dogs with sheep placental remains” (OR=1.79) for N. caninum. In addition, to presenting a significant and simultaneous seroprevalence for both agents (9.9% of the animals), the results also indicate that deficiencies in management and environmental sanitation, the presence of reservoirs, and types of exploitation enhance the seropositivity. Thus, studies like this might support sanitary programs and public policies for the prevention of T. gondii and N. caninum in the sheep herds of Paraná state.

https://doi.org/10.1590/s1984-29612021021 249 descargas

ADESÃO À TERAPIA ANTIRRETROVIRAL EM MULHERES: INFLUÊNCIA DO PERFIL CLÍNICO E COMPORTAMENTAL DE SAÚDE
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Da Rocha Cabral, Juliana

; Chianca De Andrade Moraes, Danielle

; Da Rocha Cabral, Luciana

; De Aquino Freire, Daniela

; Da Silva Abrão, Fátima Maria

; Celia De Oliveira, Regina

Ciencia y enfermería

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2021, Volumen 27 elocation 25

Resumen: En Es Pt | Texto: En Es Pt | PDF: En | PDF: Es | PDF: Pt

ABSTRACT Objective: To analyze adherence to Antiretroviral Therapy (ART) among women living with HIV from a clinical and behavioral health profile. Material and Methods: Epidemiological, descriptive, cross-sectional study, with a quantitative approach. The population consisted of women living with HIV, who are assisted by the Specialized HIV Care Services (SAEs) in five municipalities located in the I Regional Health Management Center (GERES) of the State of Pernambuco, Brazil. The sampling of the study was carried out through a non-probabilistic method (random drawing) in order to reach a representation of 30% of the total number of services enrolled in the I GERES. The sample was also set up using a non-probabilistic method by convenience, so 184 women living with HIV were part of the study. Data collection occurred between June and August 2018. The Cuestionario para la Evaluación de la Adhesión al Tratamiento Antirretroviral (CEAT-VIH) was used to measure the adherence of the participants. Results: The group that falls in the incomplete/complete higher education level has 1.69 times more prevalence of adherence to ART, non-sexually active women present 103 % chances for good adherence; and those whose medical record does not contain information on abandonment of ART: 94%. Conclusion: Considering the current challenges associated with adherence to ART, there is a clear need to develop social strategies aimed at women living with HIV in order to promote better quality of life for this population.

RESUMEN Objetivo: Analizar la adherencia a la terapia antirretroviral en mujeres viviendo con VIH desde el perfil clínico y de salud conductual. Material y Método: Estudio epidemiológico, descriptivo, transversal con abordaje cuantitativo. La población estuvo conformada por mujeres viviendo con VIH que son atendidas por Servicios de Atención Especializada (SAE) en VIH en cinco municipios ubicados en la I Gerencia Regional de Salud (GERES) de Pernambuco, Brasil. El estudio se muestreó mediante el método no probabilístico, un sorteo aleatorio, con el objetivo de alcanzar una representación del 30% del total de servicios registrados en el I GERES. Los participantes del estudio compusieron la muestra, también utilizando un método no probabilístico por conveniencia, por lo que 184 mujeres que viven con el VIH fueron parte del estudio. La recolección de datos se realizó en el intersticio entre junio y agosto de 2018. Se utilizó el Cuestionario Evaluación de la Adhesión al Tratamiento Antirretroviral (CEAT-HIV) para medir la adherencia de los participantes. Resultados: Hubo una mayor prevalencia de buena adherencia: el grupo que se ajusta al nivel de educación superior incompleta/ completa tiene 1,69 veces más prevalencia de adherencia a la Terapia Antirretroviral (TAR); las mujeres no sexualmente activas tienen un 103% más de posibilidades de una buena adherencia; y aquellos cuyos registros médicos no contienen información sobre abandono del TAR: 94%. Conclusión: A la luz de los desafíos actuales asociados a la adherencia al TAR, se ha resaltado la necesidad de desarrollar estrategias sociales dirigidas a las mujeres que viven con el VIH con el fin de promover una mejor calidad de vida para esta población.

RESUMO Objetivo: Analisar a adesão à terapia antirretroviral em mulheres vivendo com HIV a partir do perfil clínico e comportamental de saúde. Material e Método: Estudo epidemiológico, descritivo, transversal, com abordagem quantitativa. A população foi composta por mulheres que vivem com HIV que são assistidas pelos Serviços de Assistência Especializada (SAEs) em HIV em cinco municípios localizados na I Gerência Regional de Saúde (GERES) de Pernambuco, Brasil. A amostragem do estudo ocorreu por intermédio do método não probabilístico, um sorteio aleatório, com o intuito de atingir um representação de 30% do total de serviços adscritos na I GERES. Os participantes do estudo compuseram a amostra, também a partir de um método não probabilístico por conveniência, por tanto, 184 mulheres vivendo com HIV fizeram parte do estudo. A coleta de dados ocorreu no interstício compreendido entre junho a agosto de 2018. Utilizou-se o Cuestionario Evaluación de la Adhesión al Tratamiento Antirretroviral (CEAT-VIH) para mensurar a adesão das participantes. Resultados: Apresentaram maior prevalência para uma boa adesão: o grupo que se enquadra no nível de instrução superior incompleto/completo apresenta 1,69 vezes mais prevalência de adesão à Terapia Antirretroviral (TARV); mulheres não ativas sexualmente apresentam 103% de chances a mais para uma boa adesão; e aquelas em que cujo prontuário não consta informação de abandono à TARV: 94%. Conclusão: À luz dos desafios atuais associados à adesão à TARV, tem-se evidenciado a necessidade da elaboração de estratégias de cunho social destinadas a mulheres vivendo com HIV a fim de promover melhor qualidade de vida a essa população.

https://doi.org/10.29393/ce27-25atjr60025 77 descargas

10.

Differences in children and adolescents with SARS-CoV-2 infection: a cohort study in a Brazilian tertiary referral hospital
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Marques, Heloisa Helena de Sousa

; Pereira, Maria Fernanda Badue

; Santos, Angélica Carreira dos

; Fink, Thais Toledo

; Paula, Camila Sanson Yoshino de

; Litvinov, Nadia

; Schvartsman, Claudio

; Delgado, Artur Figueiredo

; Gibelli, Maria Augusta Bento Cicaroni

; Carvalho, Werther Brunow de

; Odone Filho, Vicente

; Tannuri, Uenis

; Carneiro-Sampaio, Magda

; Grisi, Sandra

; Duarte, Alberto José da Silva

; Antonangelo, Leila

; Francisco, Rossana Pucineli Vieira

; Okay, Thelma Suely

; Batisttella, Linamara Rizzo

; Carvalho, Carlos Roberto Ribeiro de

; Brentani, Alexandra Valéria Maria

; Silva, Clovis Artur

; Eisencraft, Adriana Pasmanik

; Rossi Junior, Alfio

; Fante, Alice Lima

; Cora, Aline Pivetta

; Reis, Amelia Gorete A. de Costa

; Ferrer, Ana Paula Scoleze

; Andrade, Anarella Penha Meirelles de

; Watanabe, Andreia

; Gonçalves, Angelina Maria Freire

; Waetge, Aurora Rosaria Pagliara

; Silva, Camila Altenfelder

; Ceneviva, Carina

; Lazari, Carolina dos Santos

; Abellan, Deipara Monteiro

; Santos, Emilly Henrique dos

; Sabino, Ester Cerdeira

; Bianchini, Fabíola Roberta Marim

; Alcantara, Flávio Ferraz de Paes

; Ramos, Gabriel Frizzo

; Leal, Gabriela Nunes

; Rodriguez, Isadora Souza

; Pinho, João Renato Rebello

; Carneiro, Jorge David Avaizoglou

; Paz, Jose Albino

; Ferreira, Juliana Carvalho

; Ferranti, Juliana Ferreira

; Ferreira, Juliana de Oliveira Achili

; Framil, Juliana Valéria de Souza

; Silva, Katia Regina da

; Kanunfre, Kelly Aparecida

; Bastos, Karina Lucio de Medeiros

; Galleti, Karine Vusberg

; Cristofani, Lilian Maria

; Suzuki, Lisa

; Campos, Lucia Maria Arruda

; Perondi, Maria Beatriz de Moliterno

; Diniz, Maria de Fatima Rodrigues

; Fonseca, Maria Fernanda Mota

; Cordon, Mariana Nutti de Almeida

; Pissolato, Mariana

; Peres, Marina Silva

; Garanito, Marlene Pereira

; Imamura, Marta

; Dorna, Mayra de Barros

; Luglio, Michele

; Rocha, Mussya Cisotto

; Aikawa, Nadia Emi

; Degaspare, Natalia Viu

; Sakita, Neusa Keico

; Udsen, Nicole Lee

; Scudeller, Paula Gobi

; Gaiolla, Paula Vieira de Vincenzi

; Severini, Rafael da Silva Giannasi

; Rodrigues, Regina Maria

; Toma, Ricardo Katsuya

; Paula, Ricardo Iunis Citrangulo de

; Palmeira, Patricia

; Forsait, Silvana

; Farhat, Sylvia Costa Lima

; Sakano, Tânia Miyuki Shimoda

; Koch, Vera Hermina Kalika

; Cobello Junior, Vilson

Clinics

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2021, Volumen 76 elocation e3488

Resumen: En | Texto: En | PDF: En

OBJECTIVES: To compare demographic/clinical/laboratory/treatments and outcomes among children and adolescents with laboratory-confirmed coronavirus disease 2019 (COVID-19). METHODS: This was a cross-sectional study that included patients diagnosed with pediatric COVID-19 (aged <18 years) between April 11, 2020 and April 22, 2021. During this period, 102/5,951 (1.7%) of all admissions occurred in neonates, children, and adolescents. Furthermore, 3,962 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection samples were processed in patients aged <18 years, and laboratory-confirmed COVID-19 occurred in 155 (4%) inpatients and outpatients. Six/155 pediatric patients were excluded from the study. Therefore, the final group included 149 children and adolescents (n=97 inpatients and 52 outpatients) with positive SARS-CoV-2 results. RESULTS: The frequencies of sore throat, anosmia, dysgeusia, headache, myalgia, nausea, lymphopenia, pre-existing chronic conditions, immunosuppressive conditions, and autoimmune diseases were significantly reduced in children and adolescents (p<0.05). Likewise, the frequencies of enoxaparin use (p=0.037), current immunosuppressant use (p=0.008), vasoactive agents (p=0.045), arterial hypotension (p<0.001), and shock (p=0.024) were significantly lower in children than in adolescents. Logistic regression analysis showed that adolescents with laboratory-confirmed COVID-19 had increased odds ratios (ORs) for sore throat (OR 13.054; 95% confidence interval [CI] 2.750-61.977; p=0.001), nausea (OR 8.875; 95% CI 1.660-47.446; p=0.011), and lymphopenia (OR 3.575; 95% CI 1.355-9.430; p=0.010), but also had less hospitalizations (OR 0.355; 95% CI 0.138-0.916; p=0.032). The additional logistic regression analysis on patients with preexisting chronic conditions (n=108) showed that death as an outcome was significantly associated with pediatric severe acute respiratory syndrome (SARS) (OR 22.300; 95% CI 2.341-212.421; p=0.007) and multisystem inflammatory syndrome in children (MIS-C) (OR 11.261; 95% CI 1.189-106. 581; p=0.035). CONCLUSIONS: Half of the laboratory-confirmed COVID-19 cases occurred in adolescents. Individuals belonging to this age group had an acute systemic involvement of SARS-CoV-2 infection. Pediatric SARS and MIS-C were the most important factors associated with the mortality rate in pediatric chronic conditions with COVID-19.

https://doi.org/10.6061/clinics/2021/e3488

11.

SARS-CoV-2 recombinant proteins stimulate distinct cellular and humoral immune response profiles in samples from COVID-19 convalescent patients
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Silva, Laís Teodoro da

; Ortega, Marina Mazzilli

; Tiyo, Bruna Tiaki

; Viana, Isabelle Freire Tabosa

; Lima, Tayná Evily de

; Tozetto-Mendoza, Tania Regina

; Oliveira, Luanda Mara da Silva

; Teixeira, Franciane Mouradian Emidio

; Lins, Roberto Dias

; Almeida, Alexandre de

; Mendes-Correa, Maria Cassia

; Duarte, Alberto Jose da Silva

; Oshiro, Telma Miyuki

Clinics

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2021, Volumen 76 elocation e3548

Resumen: En | Texto: En | PDF: En

OBJECTIVES: In this preliminary study we investigated cellular and humoral immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in blood samples from 14 recovered coronavirus disease 2019 (COVID-19) patients and compared them to those in samples from 12 uninfected/unvaccinated volunteers. METHODS: Cellular immunity was assessed by intracellular detection of IFN-γ in CD3+ T lymphocytes after stimulation with SARS-CoV-2 spike (S1), nucleocapsid (NC), or receptor-binding domain (RBD) recombinant proteins or overlapping peptide pools covering the sequence of SARS-CoV-2 spike, membrane and nucleocapsid regions. The humoral response was examined by ELISAs and/or chemiluminescence assays for the presence of serum IgG antibodies directed to SARS-CoV-2 proteins. RESULTS: We observed differences between humoral and cellular immune profiles in response to stimulation with the same proteins. Assays of IgG antibodies directed to SARS-CoV-2 NC, RBD and S1/S2 recombinant proteins were able to differentiate convalescent from uninfected/unvaccinated groups. Cellular immune responses to SARS-CoV-2 protein stimuli did not exhibit a specific response, as T cells from both individuals with no history of contact with SARS-CoV-2 and from recovered donors were able to produce IFN-γ. CONCLUSIONS: Determination of the cellular immune response to stimulation with a pool of SARS-CoV-2 peptides but not with SARS-CoV-2 proteins is able to distinguish convalescent individuals from unexposed individuals. Regarding the humoral immune response, the screening for serum IgG antibodies directed to SARS-CoV-2 proteins has been shown to be specific for the response of recovered individuals.

https://doi.org/10.6061/clinics/2021/e3548

12.

Posicionamento sobre COVID-19 e Gravidez em Mulheres Cardiopatas – Departamento de Cardiologia da Mulher da Sociedade Brasileira de Cardiologia – 2020
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Marques-Santos, Celi

; Avila, Walkiria Samuel

; Carvalho, Regina Coeli Marques de

; Lucena, Alexandre Jorge Gomes de

; Freire, Claudia Maria Vilas

; Alexandre, Elizabeth Regina Giunco

; Campanharo, Felipe Favorette

; Rivera, Maria Alayde Mendonça R.

; Costa, Maria Elizabeth Navegantes Caetano

; Castro, Marildes Luiza de

Arquivos Brasileiros de Cardiologia

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Nov 2020, Volumen 115 Nº 5 Paginas 975 - 986

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

https://doi.org/10.36660/abc.20201063 2767 descargas

13.

Posicionamento da Sociedade Brasileira de Cardiologia para Gravidez e Planejamento Familiar na Mulher Portadora de Cardiopatia – 2020
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Avila, Walkiria Samuel

; Alexandre, Elizabeth Regina Giunco

; Castro, Marildes Luiza de

; Lucena, Alexandre Jorge Gomes de

; Marques-Santos, Celi

; Freire, Claudia Maria Vilas

; Rossi, Eduardo Giusti

; Campanharo, Felipe Favorette

; Rivera, Ivan Romero

; Costa, Maria Elizabeth Navegantes Caetano

; Rivera, Maria Alayde Mendonça

; Carvalho, Regina Coeli Marques de

; Abzaid, Alexandre

; Moron, Antonio Fernandes

; Ramos, Auristela Isabel de Oliveira

; Albuquerque, Carlos Japhet da Mata

; Feio, Claudine Maia Alves

; Born, Daniel

; Silva, Fábio Bruno da

; Nani, Fernando Souza

; Tarasoutchi, Flavio

; Costa Junior, José de Ribamar

; Melo Filho, José Xavier de

; Katz, Leila

; Almeida, Maria Cristina Costa

; Grinberg, Max

; Amorim, Melania Maria Ramos de

; Melo, Nilson Roberto de

; Medeiros, Orlando Otávio de

; Pomerantzeff, Pablo Maria Alberto

; Braga, Sérgio Luiz Navarro

; Cristino, Sonia Conde

; Martinez, Tania Leme da Rocha

; Leal, Tatiana de Carvalho Andreuci Torres

Arquivos Brasileiros de Cardiologia

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May 2020, Volumen 114 Nº 5 Paginas 849 - 942

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https://doi.org/10.36660/abc.20200406 10784 descargas

14.

Toxoplasma gondii and Neospora caninum infections and factors associated in goats in the Parana state, Southern Brazil
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Romanelli, Paulo Roberto

; Matos, Andressa Maria Rorato Nascimento de

; Pinto-Ferreira, Fernanda

; Caldart, Eloiza Teles

; Oliveira, Juliana Silva de

; Anteveli, Gabriela

; Jeanfelice, Brenda Caroline dos Santos

; Stolf, Rafael Luiz

; Sanches, Tainá Favoreto

; Silva, Mariana Kassab e

; Minho, Alessandro Pelegrine

; Cavalcante, Antônio Cezar Rocha

; Freire, Roberta Lemos

; Mitsuka-Breganó, Regina

; Navarro, Italmar Teodorico

Revista Brasileira de Parasitologia Veterinária

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2020, Volumen 29 Nº 4 elocation e003620

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo O objetivo desse estudo foi avaliar a ocorrência de anticorpos anti-Toxoplasma gondii e anti-Neospora caninum e seus fatores associados em caprinos de propriedades do Estado do Paraná, Sul do Brasil. A análise sorológica foi realizada, utilizando-se ELISA indireto em 629 amostras de soro caprino, coletadas em 32 propriedades distribuídas em cinco mesorregiões do Estado. A soropositividade foi observada em 30,7% dos animais para T. gondii, 6,3% para N. caninum e 3,0% para ambos. Os modelos finais de regressão múltipla mostraram que o uso de reprodução assistida reduziu a chance de soropositividade para T. gondii (PR=0,70), enquanto a presença no local de abate na fazenda (PR=2,03) aumentou. Para N. caninum, o fornecimento de ração concentrada (OR=4,80) e a morte dos animais no pré-desmame (OR=9,96) aumentaram a chance de soropositividade. O estudo das variáveis associadas às soropositividades sugere deficiências no saneamento e no manejo reprodutivo dos Animais. Além disso, reconhece pontos críticos para a infecção no manejo da produção animal.

Abstract The aim of this study was to determine the occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies and their associated factors among goats in farms from the Paraná state, South Brazil. The serological analysis was performed by indirect ELISA on 629 goat serum samples collected from 32 farms distributed in five mesorregions from the state. Seropositivity was observed in 30.7% of the animals for T. gondii, 6.3% for N. caninum, and 3.0% for both agents. Final multiple regression models showed that the use of assisted reproduction decreased the chance of seropositivity for T. gondii (PR=0.70) and the slaughtering site on the farm (PR=2.03) increased it. To N. caninum, the supplying concentrated feed to animals (OR=4.80) and animal pre-weaning death (OR=9.96) increased the chance of seropositivity. The variables associated with these seropositivities suggest deficiencies in sanitation and reproductive management. In addition, it recognizes critical points of infection for animal production management.

https://doi.org/10.1590/s1984-29612020076 813 descargas

15.

Gestures in the speech-language pathology clinic: a study in the light of the materialist discourse analysis
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Kitahara, Michelle Fogaça de Oliveira

; Freire, Regina Maria Ayres de Camargo

Revista CEFAC

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2020, Volumen 22 Nº 5 elocation e17718

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: esta pesquisa procurou investigar o gesto na clínica fonoaudiológica e a ideologia dominante à luz da teoria-metodológica da análise discursiva materialista. Métodos: o procedimento para a coleta de dados foi uma entrevista semi-aberta com 12 fonoaudiólogas que atuam em diferentes campos clínicos em busca do fio discursivo que sustenta o discurso da Fonoaudiologia. A coleta de dados foi gravada em áudio e vídeo e dos dados discursivos transcritos, extraiu-se fragmentos que foram analisados sob a ótica apontada acima. Resultados: a análise discursiva indica que o fio condutor do discurso dos fonoaudiólogos é a ideologia positivista da ciência que fragmenta os sujeitos, os corpos, a linguagem e hierarquiza fala e gesto, sendo que este último se encontra subordinado ao primeiro. A materialidade da língua revela uma identificação inconsciente das terapeutas com o significante Fono-audio-logia, na medida em que o retorno à fala e a sua identidade profissional são trazidos à tona. O atendimento à surdos aparece amalgamado ao discurso sobre o gesto, em que este como forma de comunicação codificada na Língua Brasileira de Sinais- LIBRAS, representa uma ameaça ao estatuto da fala. Ainda sobre o significante fala, observou-se um deslizamento de sentidos daquele somente relacionado a articulação dos sons para aquilo que afeta o outro pela linguagem das mãos, dos gestos. Sob esta ótica, há também formações que contestam a ideologia dominante e abrem espaço para acolher o gesto na clínica, na avaliação e na terapêutica evidenciando que o gesto tem feito parte da práxis deste profissional, tanto por meio abordagens terapêuticas já conhecidas, bem como, na singularidade deste profissional evidenciando a importância e a necessidade do recrutamento do gesto na clínica fonoaudiológica. Conclusão: a crença ideológica dominante evidencia uma relação hierárquica e histórica entre fala e gesto que o exclui da clínica fonoaudiológica a medida em que representa uma ameaça ao estatuto da fala e a identidade deste profissional.

ABSTRACT Objective: this study aimed to investigate gestures in the speech-language pathology clinic and the dominant ideology in the light of the Materialist Discourse Analysis. Methods: twelve speech-language pathologists who work in different clinical fields were interviewed to investigate the main discursive thread supporting their discourse. These semi-open interviews were recorded and the discursive data were later transcribed. Fragments were extracted and analyzed from the above mentioned perspective. Results: the analysis shows that the conducting thread of the therapists’ discourse is the positivist ideology of Science, which fragments the subjects, body and language allocating speech and gesture in a hierarchy system where gesture is subordinated to the former. The language materiality shows an unconscious identification of the therapists with the signifier “Fono-Speech, Audio-Audio, Logia-Study” (Fonoaudiologia, or Speech-language Pathology in Portuguese), since the return of speech and their professional identity is brought up. From this perspective, there are formations that challenge the dominant ideology welcoming gesture as an important tool in the clinic, both in evaluation and treatment. Conclusion: the dominant ideological belief shows a hierarchical and historical association between speech and gesture that excludes gesture of the speech-language pathology clinic as it represents a threat to the speech status and the identity of this professional.

https://doi.org/10.1590/1982-0216/202022517718 429 descargas

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