Abstract For offshore structures, especially jacket supported structure such as fixed platform and offshore wind turbine, the underwater area provides an ideal site for corrosion formation and marine fouling. In this marine environment, the structures are exposed to particularly aggressive corrosion and colonized by marine organisms involving a diversity of species. In this paper, a number of cases are conducted where an existing fixed platform is subjected to uniform corrosion. Furthermore, the platform is also subjected to marine fouling. The platform is assessed to investigate the effects of corrosion and marine fouling on its structural strength by determining the Reserve Strength Ratio (RSR) value. It is found the platform is limited to at least 50 years of service life when subjected to average corrosion. However, the platform is no longer considered as safe after 37 years when subjected to severe corrosion. Moreover, there is an RSR loss of 38% when marine fouling is introduced at the first 5 years which is detrimental to the overall safety of the platform. On the other hand, there is minimum RSR loss of only 5% for most cases when marine fouling is introduced but still detrimental to the overall safety of the platform. The obtained outcomes were documented and will be useful information to conduct condition assessment (or structural health monitoring) of aged fixed jacket structures.

Stimulation by a number of conditions, including infection, cytokines, mechanical injury, and hypoxia, can upregulate inducible nitric oxide synthase (iNOS) in hepatocytes. We observed that exposure to hypergravity significantly upregulated the transcription of the hepatic iNOS gene. The aim of this study was to confirm our preliminary data, and to further investigate the distribution of the iNOS protein in the livers of mice exposed to hypergravity. ICR mice were exposed to +3 Gz for 1 h. We investigated the time course of change in the iNOS expression. Hepatic iNOS mRNA expression progressively increased in centrifuged mice from 0 to 12 h, and then decreased rapidly by 18 h. iNOS mRNA levels in the livers of centrifuged mice was significantly higher at 3, 6, and 12 h than in uncentrifuged control mice. The pattern of iNOS protein expression paralleled that of the mRNA expression. At 0 and 1 h, weak cytoplasmic iNOS immunoreactivity was found in some hepatocytes surrounding terminal hepatic venules. It was noted that at 6 h there was an increase in the number of perivenular hepatocytes with moderate to strong cytoplasmic immunoreactivity. The number of iNOS-positive hepatocytes was maximally increased at 12 h. The majority of positively stained cells showed a strong intensity of iNOS expression. The expression levels of iNOS mRNA and protein were significantly increased in the livers of mice exposed to hypergravity. These results suggest that exposure to hypergravity significantly upregulates iNOS at both transcriptional and translational levels.