ABSTRACT Junghuhnia is a cosmopolitan genus of Agaricomycetes (Basidiomycota), mostly characterized by having a dimitic hyphal system and encrusted cystidia. The genus comprises 37 legitimate species, eight of which have been reported in Brazil. This study provides updated information about the diversity and distribution of Junghuhnia in Brazil by reporting J. semisupiniformis for the first time from South America, J. globospora from Brazil, J. carneola from northeastern Brazil and the state of Pará, J. nitida from the state of Pernambuco, and J. subundata from the state of Amazonas. Descriptions of J. semisupiniformis and J. globosbora, as well a key to the accepted species of Junghuhnia from Brazil, are provided.