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Abstract The pathogenesis of systemic lupus erythematosus (SLE) is complex. Few studies in Brazilian population have addressed cell phenotypes associated with immunological responses and their associations with SLE activity. The aim of this study is to investigate cell phenotypes associated to SLE diagnosis, treatment and activity. Twenty-eight SLE female patients (17 inactive, 11 active) and 10 healthy women were included in this study. Markers of natural killer (Nk), T and B cells in peripheral blood were evaluated by flow cytometry. Nkt cells were decreased only in SLE active patients. Activated CD4+, regulatory T FoxP3+ and B cells were decreased in both active and inactive SLE patients, compared to control group. The data corroborate the disruption of immune regulatory response in SLE patients and suggest phenotipic changes as possible biomarkers of SLE activity.

https://doi.org/10.1590/s2175-97902023e20052

Prevalence of SARS-CoV-2 infection among health care workers in a reference hospital in Brazil
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Almeida, Vanessa Neves

; Cavalin, Roberta Figueiredo

; Gallo, Juliana Failde

; Guerra, Cleide Aparecida

; Madureira, Karen Cristina Rolim

; Rossi, Meire Bócoli

; Sobreira, Rozania Soeli dos Santos

; Santos, Ana Paula

; Luna, Expedito

; Lindoso, José Angelo Lauletta

Revista do Instituto de Medicina Tropical de São Paulo

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2023, Volumen 65 elocation e52

Resumen: En | Texto: En | PDF: En

ABSTRACT Health care workers (HCW) are the frontline workforce for COVID-19 patient care and, consequently, are exposed to SARS-CoV-2 infection due to close contact to infected patients. Here, we evaluate the prevalence of SARS-CoV-2 infection among HCW from an infectious disease hospital, reference center for COVID-19 care in the metropolitan area of Sao Paulo city, Brazil. Among 2,204 HCW, 1,417 (64.29%) were subjected to detection of anti-SARS-CoV-2 antibodies by chemiluminescent immunoassay. Out of the total, 271 (19.12%) presented anti-SARS-CoV-2 antibodies. Prevalence varied according to HCW categories. The highest prevalence was observed in workers from outsourced companies, cooks and kitchen assistants, hospital cleaning workers, and maintenance workers. On the other hand, resident physicians and HCW from the institution itself presented lower prevalence (nurses, nursing assistants, physicians, laboratory technicians). Social and environmental factors are important determinants, associated with exposure in the hospital environment, which can determine the greater or lesser risk of infection by pathogens that spread rapidly by air.

https://doi.org/10.1590/s1678-9946202365052

Cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid gastric administration during the pregestational and gestational periods does not influence the follicular endowment of the progeny
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Freitas, Danielle Storino de

; Lopes, Guilherme Antonio de Gouvêa

; Nascimento, Barbara Rodrigues

; Madureira, Ana Paula

; Campos-Junior, Paulo Henrique Almeida

Animal Reproduction

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2023, Volumen 20 Nº 3 elocation e20220124

Resumen: En | Texto: En | PDF: En

Abstract Fetal programming suggests that maternal stimulation and nutrition during the period of fetal development can program the progeny. Conjugated linoleic acid (CLA), an isomer of linoleic acid, has been characterized in several aspects, but few studies have been performed on its involvement in reproduction and fetal programming. The aim of this study was to evaluate the F1, F2 and F3 progeny of female mice supplemented with CLA during the pregestational and gestational periods with respect to biometric and reproductive parameters, as well as ovarian morphophysiology. The F1 progeny of mothers supplemented with CLA exhibited stable weight gain, while the F2 progeny showed no effects (P=0.0187 and P=0.0245, respectively). A reduction in Lee's Index was observed in both generations at the second post-weaning evaluation week in the animals treated with CLA (P=0.0100 and P=0.0078, respectively). The F2 generation showed an increase in the anogenital index in both sexes of the animals treated with CLA (P= 0.0114 and P<0.0001, female and male respectively). CLA administration to mothers did not affect any of the following in their progeny: ovarian follicle mobilization (P>0.05), follicle number (P>0.05) and the integrated density of the lipid content of oocytes included in antral follicles (P>0.05). This study evaluated the use of CLA in mothers and found that it did not affect the progeny regarding murine reproductive performance, suggesting that this supplement can be used safely.

https://doi.org/10.1590/1984-3143-ar2022-0124

Tissue healing changes on wounds in rats after treatment with Hancornia speciosa latex in cream-gel formulation
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D’Abadia, Patrícia Lima

; Lemes, Susy Ricardo

; Melo-Reis, Paulo Roberto de

; Lino Júnior, Ruy de Souza

; Gonçalves, Pablo José

; Reis, Diego dos Santos

; Caixeta, Graziele Alícia Batista

; Amaral, Vanessa Cristine Santana

; Almeida, Luciane Madureira

Acta Cirúrgica Brasileira

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2022, Volumen 37 Nº 10 elocation e371001

Resumen: En | Texto: En | PDF: En

ABSTRACT Purpose: Hancornia speciosa latex has shown pharmacological potential in wound healing processes due to its angiogenic, osteogenic, and anti-inflammatory activities. The aims of this study were to carry out a cream-gel formulation with 5, 10 and 25% of H. speciosa serum latex and to evaluate its potential to stimulate the skin regeneration in rats’ wounds. Methods: One hundred and twenty rats were divided into five groups: neutral control with saline (G1), cream-gel based on H. speciosa latex serum at 5% m/v (G2), cream-gel at 15% m/v (G3), cream-gel at 25% m/v (G4), and cream-gel (G5). The animals were euthanized at three, seven, 14 and 21 days after the injury induction, and some parameters were analyzed: wound contraction, necrosis, fibrin, polymorphonuclear and mononuclear infiltrates, fibroblast, angiogenesis, hemorrhage, and collagen. Results: The therapeutic treatment with cream-gel at 15 and 25% is beneficial in the inflammatory phase of healing processes since it increased the angiogenesis and proliferation of mononuclear infiltrations in wounds. Regarding wound contraction, the treatment with cream-gel (5 and 15%) induced a higher rate of contraction in the proliferative phase. The 15% cream-gel formulation stimulated a greater production of collagen in the injured tissues. Conclusions: H. speciosa cream-gel is a low-cost herbal medicine which can aid in tissue repair.

https://doi.org/10.1590/acb371001

COVID-19 in the Perioperative Period of Cardiovascular Surgery: the Brazilian Experience
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Gomes, Walter J.

; Rocco, Isadora

; Pimentel, Wallace S.

; Pinheiro, Aislan H. B.

; Souza, Paulo M. S.

; Costa, Luiz A. A.

; Teixeira, Marjory M. P.

; Ohashi, Leonardo P.

; Bublitz, Caroline

; Begot, Isis

; Moreira, Rita Simone L

; Hossne Jr, Nelson A.

; Vargas, Guilherme F.

; Branco, João Nelson R.

; Teles, Carlos A.

; Medeiros, Eduardo A. S.

; Sáfadi, Camila

; Rampinelli, Amândio

; Moratelli Neto, Leopoldo

; Rosado, Anderson Rosa

; Mesacasa, Franciele Kuhn

; Capriata, Ismael Escobar

; Segalote, Rodrigo Coelho

; Palmieri, Deborah Louize da Rocha Vianna

; Jardim, Amanda Cristina Mendes

; Vianna, Diego Sarty

; Coutinho, Joaquim Henrique de Souza Aguiar

; Jazbik, João Carlos

; Coutinho, Henrique Madureira da Rocha

; Kikuta, Gustavo

; Almeida, Zely Sant'Anna Marotti de

; Feguri, Gibran Roder

; Lima, Paulo Ruiz Lucio de

; Franco, Anna Carolina

; Borges, Danilo de Cerqueira

; Cruz, Felipe Ramos Honorato De La

; Croti, Ulisses Alexandre

; Borim, Bruna Cury

; Marchi, Carlos Henrique De

; Goraieb, Lilian

; Postigo, Karolyne Barroca Sanches

; Jucá, Fabiano Gonçalves

; Oliveira, Fátima Rosane de Almeida

; Souza, Rafael Bezerra de

; Zilli, Alexandre Cabral

; Mas, Raul Gaston Sanchez

; Bettiati Junior, Luiz Carlos

; Tranchesi, Ricardo

; Bertini Jr, Ayrton

; Franco, Leandro Vieira

; Fernandes, Priscila

; Oliveira, Fabiana

; Moraes Jr, Roberto

; Araújo, Thiago Cavalcanti Vila Nova de

; Braga, Otávio Penna

; Pedrosa Sobrinho, Antônio Cavalcanti

; Teixeira, Roberta Tavares Barreto

; Camboim, Irla Lavor Lucena

; Gomes, Eduardo Nascimento

; Reis, Pedro Horigushi

; Garcia, Luara Piovan

; Scorsioni, Nelson Henrique Goes

; Lago, Roberto

; Guizilini, Solange

Brazilian Journal of Cardiovascular Surgery

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Dic 2021, Volumen 36 Nº 6 Paginas 725 - 735

Resumen: En | Texto: En | PDF: En

Abstract Introduction: We investigated the clinical course and outcomes of patients submitted to cardiovascular surgery in Brazil and who had developed symptoms/signs of coronavirus disease 2019 (COVID-19) in the perioperative period. Methods: A retrospective multicenter study including 104 patients who were allocated in three groups according to time of positive real time reverse transcriptase-polymerase chain reaction (RT-PCR) for the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2): group 1, patients who underwent cardiac surgery > 10 days after positive RT-PCR; group 2, patients with a positive RT-PCR within 10 days before or after surgery; group 3, patients who presented positive RT-PCR > 10 days after surgery. The primary outcome was mortality and secondary outcomes were postoperative complications, intensive care unit (ICU) length of stay, and postoperative days of hospitalization. Results: The three groups were similar with respect to age, the European System of Cardiac Operative Risk Evaluation score, and comorbidities, except hypertension. Postoperative complications and death were significantly higher in groups 2 and 3 than in group 1, and no significant difference between groups 2 and 3 was seen. Group 2 showed a high prevalence of surgery performed as an urgent procedure. Although no significant differences were observed in ICU length of stay, total postoperative hospitalization time was significantly higher in group 3 than in groups 1 and 2. Conclusion: COVID-19 affecting the postoperative period of patients who underwent cardiovascular surgery is associated with a higher rate of morbidity and mortality. Delaying procedures in RT-PCR-positive patients may help reduce risks of perioperative complications and death.

https://doi.org/10.21470/1678-9741-2021-0960

COVID-19 vaccination campaign: dialogues with nurses working in Primary Health Care
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Souza, Jeane Barros de

; Potrich, Tassiana

; Bitencourt, Julia Valeria de Oliveira Vargas

; Madureira, Valéria Silvana Faganello

; Heidemann, Ivonete Teresinha Schülter Buss

; Menegolla, Gisielle Christine Schmidt

Revista da Escola de Enfermagem da USP

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2021, Volumen 55 elocation e20210193

Resumen: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivo: Refletir sobre a percepção de enfermeiros atuantes na Atenção Primária à Saúde sobre o desenvolvimento da campanha de vacinação contra a COVID-19. Método: Estudo qualitativo, tipo ação participante, fundamentado nos preceitos teóricos metodológicos de Paulo Freire, que consiste em três fases interligadas: Investigação Temática, Codificação e Descodificação, Desvelamento Crítico. Realizaram-se três Círculos de Cultura virtual, entre fevereiro e março de 2021, com 11 enfermeiros da Atenção Primária à Saúde, residentes em Santa Catarina, Brasil. Resultados: Os enfermeiros definiram dois temas para discussão no Círculo de Cultura: 1. Encontros na campanha de vacinação COVID-19, representando as potencialidades; 2. Desencontros na campanha de vacinação COVID-19, em que desvelaram as fragilidades. Conclusão: Os enfermeiros apontaram potencialidades como a atuação em equipe, com esperança, motivação e alegria na operacionalização da campanha de vacinação, por meio de ações inovadoras e antigas. Também evidenciaram fragilidades como falta de formação específica e comunicação, dificuldades com os registros e a aplicação do imunobiológico, salientando o movimento antivacina e o intenso trabalho, o que carece de ajustes em prol da qualificação da assistência à população.

ABSTRACT Objective: To reflect on the perception of nurses working in Primary Health Care about the development of the COVID-19 vaccination campaign. Method: This is a participatory action qualitative study, based on the methodological theoretical precepts by Paulo Freire, which consists of three interconnected phases: Thematic Research, Coding and Decoding, and Critical Unveiling. Three virtual Culture Circles were held between February and March 2021, with 11 nurses from Primary Health Care, residing in Santa Catarina, Brazil. Results: Nurses defined two themes for discussion in the Culture Circle: 1. Matches in the COVID-19 vaccination campaign, representing the potentialities; 2. Mismatches in the COVID-19 vaccination campaign, in which the weaknesses were revealed. Conclusion: Nurses indicated potentialities such as teamwork, with hope, motivation, and joy in the vaccination campaign operationalization, through innovative and old actions. They also highlighted weaknesses such as lack of specific training and communication, difficulties with recording and application of the immunobiological, mentioning the anti-vaccine movement and the intense work, which requires adjustments to improve assistance to the population.

RESUMEN Objetivo: Reflexionar sobre la percepción de enfermeros actuantes en la Atención Primaria de Salud sobre el desarrollo de la campaña de vacunación contra COVID-19. Método: Estudio cualitativo, tipo acción participante, fundamentado en los preceptos teóricos metodológicos de Paulo Freire, que consiste en tres etapas interconectadas: Investigación Temática, Codificación y Descodificación; Revelación Crítica. Se realizaron tres Encuentros de Cultura virtual, entre febrero y marzo de 2021, con 11 enfermeros de la Atención Primaria de Salud, residentes en Santa Catarina, Brasil. Resultados: Los enfermeros definieron dos temas para debate en el Encuentro de Cultura: 1. Logros en la campaña de vacunación contra COVID-19, en que revelaron las potencialidades; 2. Dificultades en la campaña de vacunación contra COVID-19, en que revelaron las vulnerabilidades. Conclusión: Los enfermeros destacaron potencialidades como el trabajo en equipo, con esperanza, motivación y alegría en la operacionalización de la campaña de vacunación, por medio de acciones innovadoras y antiguas. También destacaron vulnerabilidades como ausencia de formación específica y comunicación, dificultades con los registros y aplicación del inmunobiológico, evidenciando el movimiento antivacuna y el intenso trabajo, el que demanda más ajustes para lograr calificar la asistencia a la población.

https://doi.org/10.1590/1980-220x-reeusp-2021-0193 619 descargas

PERCEPÇÕES DE IDOSOS SOBRE O ENFRENTAMENTO DA COVID-19
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Luzardo, Adriana Remião

; Souza, Jeane Barros de

; Bitencourt, Julia Valeria de Oliveira Vargas

; Maestri, Eleine

; Madureira, Valéria Silvana Faganello

; Biffi, Priscila

Cogitare Enfermagem

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2021, Volumen 26 elocation e78852

Resumen: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Objetivo compreender a percepção de idosos sobre o enfrentamento da COVID-19. Método pesquisa qualitativa do tipo ação-participante, com base teórica fundamentada em Paulo Freire, adaptada à realidade da pandemia. Percorreram-se as etapas do Itinerário de Pesquisa com analogia ao vinho: Investigação Temática à cor; Codificação-Descodificação à doçura e acidez; Desvelamento Crítico ao álcool. Realizou-se um Círculo de Cultura virtual em agosto de 2020, incluindo 23 idosos de um Centro de Convivência do Sul do Brasil. Resultados Temas Geradores: 1) Repercussões amargas da COVID-19, destacando-se medos, insegurança, ansiedade, falta do convívio com pessoas e do cotidiano antes do isolamento; 2) Doces repercussões da COVID-19, desvelando-se esperança, fé, reinvenção da vida, aprendizados, sentir-se amado, cuidado e apoiado mesmo à distância. Conclusão a manutenção da capacidade física e mental dos idosos diante de tragédias sociais deve pautar-se por diretrizes em protocolos de cuidado integral, para prevenção de agravos e promoção da saúde.

ABSTRACT Objective to understand older adults’ perceptions about how to face COVID-19. Method a qualitative research study of the participant-action type, with its theoretical basis grounded on Paulo Freire, adapted to the reality of the pandemic. The Research Itinerary stages were followed with an analogy to wine: Thematic Research for color; Coding-Decoding for sweetness and acidity; and Critical Unveiling for alcohol. A virtual Culture Circle was held in August 2020, including 23 older adults from a Cohabitation Center in southern Brazil. Results Generating Themes: 1) Bitter repercussions of COVID-19, especially fears, insecurity, anxiety, lack of interaction with people, and missing how daily life was before isolation; 2) Sweet repercussions of COVID-19, unveiling hope, faith, reinvention of life, lessons learned, feeling loved, cared for and supported, even if at a distance. Conclusion maintenance of the older adults’ physical and mental capacity in the face of social tragedies must be guided by guidelines on comprehensive care protocols to prevent diseases and promote health.

RESUMEN Objetivo comprender la percepción de los adultos mayores sobre cómo hacer frente al COVID-19. Método investigación cualitativa del tipo acción-participante, con su base teórica fundamentada en Paulo Freire, adaptada a la realidad de la pandemia. Las etapas do Itinerario de Investigación se realizaron por medio de una analogía con el vino: la Investigación Temática se refirió al color; la Codificación-Decodificación hizo referencia a la dulzura y la acidez; y la Revelación Crítica se vinculó al alcohol. Se organizó un Círculo de Cultura virtual en agosto de 2020, del que participaron 23 adultos mayores de un Centro de Convivencia del sur do Brasil. Resultados Temas Generadores: 1) Repercusiones amargas del COVID-19, entre las que se destacaron las siguientes: miedos, inseguridad, ansiedad, falta de interacción con las personas y con la rutina previa al aislamiento; 2) Repercusiones dulces del COVID-19, entre las que se revelaron esperanza, fe, reinvención de la vida, aprendizajes, y sentirse amado, cuidado y apoyado, incluso a la distancia. Conclusión la preservación de la capacidad física y mental de los adultos mayores frente a tragedias sociales debe basarse en pautas incluidas en protocolos de atención integral, para prevenir problemas y promover la salud.

https://doi.org/10.5380/ce.v26i0.78852

Safety and immunogenicity of an inactivated SARS-CoV-2 vaccine (CoronaVac) in inadvertently vaccinated healthy children
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Fernandes, Eder Gatti

; López-Lopes, Giselle Ibette Silva

; Silva, Valeria Oliveira

; Yamashiro, Rosemeire

; Madureira, Karen Cristina Rolim

; Gallo, Juliana Failde

; Lindoso, José Angelo

; Sato, Helena Keico

; de Araujo, Núbia Virginia D’Avila Limeira

; Nerger, Maria Ligia Bacciotte Ramos

; Brigido, Luis Fernando Macedo

Revista do Instituto de Medicina Tropical de São Paulo

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2021, Volumen 63 elocation e83

Resumen: En | Texto: En | PDF: En

ABSTRACT Twenty-seven children aged seven months to 5 years were inadvertently vaccinated with a COVID-19 vaccine, the CoronaVac (Sinovac, China), an inactivated SARS-CoV-2 vaccine, in two different cities of Sao Paulo State, Brazil. After the event, these children were monitored by local pediatricians and serum samples were collected at the first visit and 30 days after vaccination and tested for SARS-CoV-2 S1 serology with Ortho total IgG anti-S1 protein and Cpass, an ACE2 receptor binding domain inhibition assay. Only one child had a mild symptom after vaccination, with no other adverse events documented up to the 30 days follow-up. Of 27 children tested 3-9 days after vaccination, 5 (19%) had positive serology suggesting a previous natural SARS-CoV-2 infection, with all 19 tested on day 30 after vaccination and presenting with positive tests, with an increment of antibody titers in those initially positive. A low Cpass binding inhibition was observed in the first collection in 11 seronegative cases, with high titers among those anti-S1 positive. All children showed an important increase in antibody titers on day 30. The event allowed the documentation of a robust serological response to one dose of CoronaVac in this small population of young children, with no major adverse effects. Although it was an unfortunate accident, this event may contribute with future vaccine strategies in this age group. The data suggest that CoronaVac is safe and immunogenic for children.

https://doi.org/10.1590/s1678-9946202163083

Pandemia e imigração: famílias haitianas no enfrentamento da COVID-19 no Brasil
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Souza, Jeane Barros de

; Heidemann, Ivonete Terezinha Schülter Buss

; Geremia, Daniela Savi

; Madureira, Valéria Silvana Faganello

; Bitencourt, Julia Valeria de Oliveira Vargas

; Tombini, Larissa Hermes Thomas

Escola Anna Nery

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2020, Volumen 24 Nº spe elocation e20200242

Resumen: En Es Pt | Texto: En Es Pt | PDF: En | PDF: Es | PDF: Pt

Abstract: Objective to understand the experience of coping and repercussions of COVID-19 from the perspective of Haitian immigrant families in Brazil. Method qualitative, action-participant study, based on Paulo Freire's Research Itinerary, which has three phases: Thematic Research; Encoding and Decoding; Critical Unveiling. A Virtual Culture Circle was held in May 2020, with 10 families of Haitian immigrants residing in western Santa Catarina. Results the participants discussed concerns generated in facing the pandemic: work and family subsistence in Brazil and Haiti; uncertainty of the future; risk of contamination and dying in Brazil; cancellation of children's classes; discouragement and loneliness. They revealed opportunities in experiencing the pandemic: help received; personal and family strength; rethinking life; trust in God and hope. Conclusions and implications for practice Haitian immigrants are in a situation of social, economic and mental health vulnerability when facing COVID-19. The identification of this vulnerability considering social, economic and cultural factors is fundamental to the proposition of public policies and the adoption of effective strategies to face the situation. The Virtual Culture Circle expands possibilities for nursing, as it enables the interactions necessary for health promotion, even in the face of the pandemic.

Resumen Objetivo comprender la experiencia de afrontamiento y las repercusiones de COVID-19 desde la perspectiva de las familias de inmigrantes haitianos en Brasil. Método estudio cualitativo, de tipo acción-participante, basado en el Itinerario de Investigación de Paulo Freire, que tiene tres fases: Investigación temática; Codificación y decodificación; Revelación crítica. En mayo de 2020 se realizó un Círculo de Cultura Virtual, con 10 familias de inmigrantes haitianos que residen en el oeste de Santa Catarina. Resultados los participantes discutieron las preocupaciones generadas al enfrentar la pandemia: el trabajo y la subsistencia familiar en Brasil y Haití; la incertidumbre del futuro; el riesgo de contaminación y muerte en Brasil; la cancelación de las clases infantiles; el desánimo y la soledad. Revelaron oportunidades en la experiencia de la pandemia: la ayuda recibida; fortaleza personal y familiar; el repensar la vida; la confianza en Dios y la esperanza. Conclusiones e implicaciones para la práctica los inmigrantes haitianos se encuentran en una situación de vulnerabilidad social, económica y de salud mental en la confrontación de COVID-19. La identificación de esta vulnerabilidad, considerando los factores sociales, económicos y culturales es fundamental para la propuesta de políticas públicas y la adopción de estrategias efectivas para enfrentar la situación. El Círculo de Cultura Virtual amplía las posibilidades de la enfermería, ya que permite las interacciones necesarias para la promoción de la salud, incluso ante la pandemia.

Resumo Objetivo compreender a vivência do enfrentamento e repercussões da COVID-19 na perspectiva das famílias de imigrantes haitianos no Brasil. Método estudo qualitativo, do tipo ação-participante, fundamentado no Itinerário de Pesquisa de Paulo Freire, que possui três fases: Investigação Temática; Codificação e Descodificação; Desvelamento Crítico. Foi realizado Círculo de Cultura Virtual em maio de 2020, com 10 famílias de imigrantes haitianos, residentes no oeste de Santa Catarina. Resultados os participantes discutiram preocupações geradas no enfrentamento da pandemia: trabalho e subsistência da família no Brasil e no Haiti; incerteza do futuro; risco de contaminação e de morrer no Brasil; cancelamento das aulas dos filhos; desânimo e solidão. Desvelaram oportunidades na vivência da pandemia: ajuda recebida; força pessoal e familiar; repensar a vida; confiança em Deus e esperança. Conclusões e implicações para a prática os imigrantes haitianos se encontram em situação de vulnerabilidade social, econômica e de saúde mental no enfrentamento da COVID-19. A identificação dessa vulnerabilidade, considerando fatores sociais, econômicos e culturais é fundamental à proposição de políticas públicas e adoção de estratégias efetivas de enfrentamento da situação. O Círculo de Cultura Virtual amplia possibilidades para a enfermagem, pois possibilita as interações necessárias à promoção da saúde, mesmo diante da pandemia.

https://doi.org/10.1590/2177-9465-ean-2020-0242 2 descargas

10.

A phonetic study of Zootopia characters’ voices in Brazilian Portuguese dubbing: the role of stereotypes
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Crochiquia, Alice

; Eriksson, Anders

; Fontes, Mario A. S.

; Madureira, Sandra

DELTA: Documentação de Estudos em Lingüística Teórica e Aplicada

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2020, Volumen 36 Nº 3 elocation 2020360311

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Este trabalho segue uma abordagem experimental de investigação da fala expressiva que integra procedimentos metodológicos de análises perceptiva e acústica. Como objeto deste trabalho, focalizamos a qualidade de voz e a dinâmica vocal de personagens com distintas personalidades. Amostras de fala de quatro principais personagens do filme de animação intitulado “Zootopia” dublados por atores brasileiros são analisadas. Devido à função expressiva da qualidade de voz, a seguinte pergunta de pesquisa foi aventada: Que tipos de ajustes de qualidade de voz e de dinâmica vocal foram usados pelos atores na dublagem de “Zootopia” para compor os perfis vocais dos personagens? A avaliação perceptiva de 54 estímulos de fala foi realizada com o Sistema de Avaliação Vocal (Laver & Mackenzie Beck, 2007). Medidas acústicas foram automaticamente extraídas, aplicando o ExpressionEvaluator script (Barbosa, 2008) no PRAAT. Os perfis de cada um dos quatro personagens foram compostos com base nas características psicológicas descritas no roteiro do filme. Os resultados da análise acústica, da análise perceptiva dos ajustes de qualidade vocal e de dinâmica vocal foram correlacionados por meio do método MFA (Análise Multifatorial) no ambiente R. Foram utilizadas 40 variáveis cuja análise resultou na distribuição dos estímulos em 6 clusters. As variáveis quantitativas que apresentaram a maior percentagem de correlação foram as relacionadas à frequência fundamental e à inclinação espectral: Standard Deviation of f0 Derivative, Standard Deviation of Spectral Tilt e f0 Median. As variáveis qualitativas com maior grau de correlação foram: Lowered Larynx, Lip Rounding, Breathy Voice e Minimised Pitch Range. A pesquisa apresentou evidências a favor do uso simbólico da matéria fônica e contribuições para o entendimento de como os estereótipos são estabelecidos.

ABSTRACT This work comprises an experimental investigation approach of expressive speech that integrates methodological procedures of perceptual and acoustic analyses. As the object of this work, we have focused on voice quality and vocal dynamics. Speech samples from the four main personality-distinct characters in the animated feature film “Zootopia” dubbed by Brazilian voice actors have been analysed. Due to the expressive function of voice quality, we have posed the following question: what types of voice quality and vocal dynamics settings were used by the voice actors in the Brazilian dubbing of “Zootopia” to compose the vocal profiles of the characters? Perceptual evaluation of the 54 speech stimuli was performed using the Vocal Profile Analysis protocol (Laver & Mackenzie Beck, 2007). Acoustic measures were automatically extracted using the ExpressionEvaluator script (Barbosa, 2008) for PRAAT. The profiles for each of the four characters were composed based on the psychological traits described in the film script. The results of the acoustic analysis, the perceptual analysis of voice quality and vocal dynamics settings were correlated using the MFA (Multiple Factor Analysis) method in the R environment based on 40 variables (quantitative and qualitative) and it turned out that the speech stimuli were distributed in 6 clusters according to the variables analysed. The quantitative variables that presented the highest correlation percentage were: Standard Deviation of f0 Derivative, Standard Deviation of Spectral Tilt, f0 Median. The qualitative variables that presented the highest correlation percentage were: Lowered Larynx, Lip Rounding, Breathy Voice and Minimised Pitch Range. The research has presented evidence in favor of the symbolic use of phonic matter and contributions to the understanding of how vocal stereotypes are established.

https://doi.org/10.1590/1678-460x2020360311 587 descargas

11.

Pandemia e imigração: famílias haitianas no enfrentamento da COVID-19 no Brasil
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Souza, Jeane Barros de

; Heidemann, Ivonete Terezinha Schülter Buss

; Geremia, Daniela Savi

; Madureira, Valéria Silvana Faganello

; Bitencourt, Julia Valeria de Oliveira Vargas

; Tombini, Larissa Hermes Thomas

Escola Anna Nery

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2020, Volumen 24 Nº spe elocation e20200242

Resumen: Pt En Es | Texto: Pt En Es | PDF: Pt | PDF: En | PDF: Es

https://doi.org/10.1590/2177-9465-ean-2020-0242 1670 descargas

12.

Pneumonia risk factors in stroke patients
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Mariano, Paulo Mateus Madureira Soares

; Rodrigues, Mateus de Sousa

; Santana, Leonardo Fernandes e

; Guimarães, Manoel Pereira

; Schwingel, Paulo Adriano

; Gomes, Orlando Vieira

; Moura, José Carlos de

Revista CEFAC

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2020, Volumen 22 Nº 6 elocation e9920

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Objetivo: avaliar as características demográficas e clínicas associadas ao desenvolvimento de pneumonia em pacientes após acidente vascular encefálico internados em um hospital terciário do Vale do São Francisco que atende os estados de Pernambuco e Bahia, Brasil. Métodos: trata-se de estudo unicêntrico, observacional, analítico e transversal realizado a partir dos prontuários de pacientes com diagnóstico de acidente vascular encefálico incluídos no Registro de Acidente Vascular Encefálico (Estudo RAVESS). A análise estatística foi composta pelos testes qui-quadrado, exato de Fisher e a análise de variância com pós-teste de Bonferroni com nível de P≤0,05. Resultados: foram coletados dados de 69 pacientes com AVE agudo com média±desvio padrão de idade de 63,2±16,8 anos, sendo 37 (53,6%) do sexo feminino, com prevalência de pneumonia durante o internamento estimada em 31,9% (Intervalo de Confiança de 95%: 21,2-44,2%). Na análise univariada para preditores de pneumonia após acidente vascular encefálico foram identificados: idade mais avançada (72,6±17,9 vs. 58,8±14,5; P=0,001), menor sinal de resposta a escala de coma de Glasgow durante a admissão (11,3±1,8 vs. 13,3±2,1; P=0,001) e maior frequência de disartria na admissão (61,9% vs. 27,9%; P=0,009). Conclusão: a pneumonia foi uma complicação prevalente em pacientes após acidente vascular encefálico de um hospital terciário brasileiro, tendo sido relacionada com idade avançada do paciente e a severidade do evento encefálico.

ABSTRACT Purpose: to assess the demographic and clinical characteristics associated with the development of pneumonia in post-stroke patients hospitalized in a tertiary hospital, located in the Vale do São Francisco, that covers the states of Pernambuco and Bahia, Brazil. Methods: a unicentric, observational, analytical, cross-sectional study, based on the medical records of patients diagnosed with stroke and included in the Stroke Registry (RAVESS study). The statistical analysis was made with the chi-square test, Fisher’s exact test, and the analysis of variance, with the Bonferroni’s post-test, and P≤0.05. Results: data from 69 patients presented with acute stroke were collected, aged 63.2±16.8 years; 37 (53.6%) were females; the prevalence of pneumonia during hospital stay was estimated at 31.9% (95% confidence interval: 21.2-44.2%). In the univariate analysis of predictors for post-stroke pneumonia, the following were identified: older age (72.6±17.9 vs. 58.8±14.5; P = 0.001), lower response signal to the Glasgow Coma Scale at admission (11.3±1.8 vs. 13.3±2.1; P = 0.001), and higher frequency of dysarthria at admission (61.9% vs. 27.9%; P = 0.009). Conclusion: pneumonia was a prevalent complication in post-stroke patients at a Brazilian tertiary hospital. It was related to the patient’s older age and the severity of the cerebral event.

https://doi.org/10.1590/1982-0216/20202269920 1297 descargas

13.

Effect of the type of milking on mammary gland examination in Saanen goats
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Ramos, Jean Silva

; Gomes, Viviani

; Matazo, Mariana Porto

; Silva, Cynthia Pereira da Costa e

; Baldacim, Vinicius Alvim Passos

; Madureira, Karina Medici

Arquivos do Instituto Biológico

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2020, Volumen 87 elocation e0802018

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Esta pesquisa avaliou o efeito da ordenha sobre o exame físico da glândula mamária (GM) e do leite em cabras Saanen. Seis propriedades foram selecionadas, quatro fazendas foram utilizadas após avaliação da seleção dos critérios e distribuídas em dois grupos de acordo com o método de ordenha: manual ou mecânico. O exame físico da GM foi realizado utilizando as técnicas de inspeção e palpação, além do exame do leite pelo teste da caneca de fundo escuro, California Mastitis Test, contagem de células somáticas e exame bacteriológico. Foi possível detectar uma associação entre o método de ordenha com a maioria dos exames físicos da GM: inspeção da GM (p = 0,001), inspeção do teto (p = 0,002), palpação da GM (p = 0,054) e palpação do teto (p = 0,036). A ordenha mecânica associou-se com a redução do volume de GM (OR = 6,00), redução do tamanho do teto (OR = 16,19) e consistência firme do parênquima mamário (OR = 2,39). O uso da ordenha mecânica representou menor risco para o aumento do volume do volume da GM (OR = 0,288) e presença da cisternite (OR = 0,490). Também foi detectada a associação entre a CCS e o tipo de ordenha (p = 0,002). A ordenha mecânica foi associada com as alterações no exame físico de GM e do leite compatíveis com lesões traumáticas. Essas informações indicam o uso inadequado do equipamento de ordenha na criação de cabras leiteiras.

ABSTRACT This research evaluated the effect of milking on the physical exam of the mammary gland (MG) and milk examination in Saanen goats. Six properties were selected, four farms were used after evaluation of criteria selection and distributed in two groups according to the milking method: manual or mechanical. The physical examination of MG was performed using the techniques of inspection and palpation, in addition to the examination of the milk by strip cup, California Mastitis Test, somatic cell count, and bacteriological exam. It was possible to detect an association between the milking method with the most of physical exam of the MG: inspection of the MG (p = 0.001), inspection of the teat (p = 0.002), palpation of the MG (p = 0.054) and palpation of the teat (p = 0.036). The machine milking had an association with the reduction of the volume of MG (OR = 6.00), reduction of the teat size (OR = 16.19), and firm consistency of mammary parenchyma (OR = 2.39). The use of machine milking represented a less risk for an increase of the volume of the MG volume (OR = 0.288) and the presence of cisternitis (OR = 0.490). The association between the SCC and the type of milking was also detected (p = 0.002). Machine milking was associated with changesin the physical exam of MG and milk examination compatible with traumatic lesions. This information indicates inappropriate use of milking equipment in the dairy goat’s properties.

https://doi.org/10.1590/1808-1657000802018 671 descargas

14.

Do “buraco” ao atrativo turístico: uma sociologia da ressignificação do rural
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Martins, Rodrigo Constante

; Madureira, Gabriel Alarcon

Revista de Economia e Sociologia Rural

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Jun 2019, Volumen 57 Nº 2 Paginas 326 - 338

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo: O objetivo do artigo é discutir as transformações contemporâneas do rural a partir dos fenômenos da ressignificação da natureza e da questão ambiental. O trabalho toma como base um estudo de caso sobre as representações da natureza no município de Brotas, situado na região central do estado de São Paulo. Neste município, a afinidade eletiva entre profissionalização do turismo de aventura, questão ambiental e ressignificação discursiva do rural foi responsável por um importante movimento de transformação da localidade entre 1990 e a primeira década do século XXI. O tema específico a ser discutido neste caso será a criação de um “objeto de natureza” que passou a caracterizar o rural no município – a saber, as cachoeiras, anteriormente estigmatizadas como “buracos” e posteriormente transformadas em atrativos ambientais dotados de valor econômico.

Abstract: This article aims to discuss the contemporary transformations of the rural based on the resignification phenomenon of nature and the environment. The article will be based on a case study about the representations of nature in Brotas, city located in the central region of São Paulo state. In this city, the elective affinity between the professionalization of adventure tourism, environmental issues and the discursive resignification of the rural was responsible for an important transformation of the locality from the 1990s to the first decade of the 21st century. The specific theme to be discussed in this case is the creation of an “object of nature” which has begun to characterize the rural in Brotas – namely the waterfalls, that previously was stigmatized as “holes”, and later transformed into environmental attractions endowed with economic value.

https://doi.org/10.1590/1806-9479.2019.185693 827 descargas

15.

Molecular detection of Leishmania spp. in cattle from Brazil by means of PCR using internal transcribed spacer 1
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Vioti, Geovanna

; Leonel, João Augusto Franco

; Lemes, Kleber Menegon

; Pereira, Vanessa Figueredo

; Ferreira, Helena Lage

; Keid, Lara Borges

; Madureira, Ed Hoffman

; Soares, Rodrigo Martins

; Benassi, Júlia Cristina

; Oliveira, Trícia Maria Ferreira de Sousa

Revista Brasileira de Parasitologia Veterinária

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Abr 2019, Volumen 28 Nº 2 Paginas 303 - 305

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Leishmania spp. são agentes causadores das leishmanioses em humanos e em animais, gerando grande impacto à saúde pública. Este estudo objetivou detectar a circulação de Leishmania spp. em área não endêmica para leishmaniose visceral de São Paulo, Brasil. Foram extraídas amostras de DNA de 100 novilhas da cidade de Pirassununga. Estas amostras foram amplificadas com os iniciadores específicos para tripanosomatídeos Internal Transcriber Spacer 1 (ITS1). Os ensaios revelaram uma amostra com bandas entre 300 e 350 pares de base (pb). A amostra demonstrou 100% de identidade com Leishmania infantum (314 pb). Os resultados sugerem que o gado pode ser infectado por L. infantum no Brasil.

Abstract Leishmania spp. are important agents of human and animal leishmaniases that have an important impact on public health. In this study, we aimed to detect the circulation of Leishmania spp. in cattle from a visceral leishmaniasis non-endemic area of the state of São Paulo, Brazil. DNA was extracted from blood samples from 100 heifers in the municipality of Pirassununga and was amplified using primers specific for the first internal transcriber spacer (ITS1), to assess the presence of trypanosomatids. The assays revealed that one sample presented bands of between 300 and 350 base pairs. In GenBank, this sample matched 100% with Leishmania infantum (314 base pairs). The results suggest that cattle can be infected by Leishmania infantum in Brazil.

https://doi.org/10.1590/s1984-29612019003 1028 descargas

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