RESUMO Objetivo: Analisar a associação entre as características fenotípicas, genotípicas e a gravidade da doença em indivíduos com fibrose cística atendidos em um centro de referência de Minas Gerais, Brasil. Métodos: Trata-se de um estudo retrospectivo, em que os dados clínicos, laboratoriais, as manifestações respiratórias e gastrointestinais, o tipo de tratamento, o escore de Shwachman-Kulczycki e as mutações foram coletados dos prontuários de registros dos pacientes. Resultados: A amostra incluiu 50 participantes, de um a 33 anos de idade, sendo 50% do sexo feminino. Do total de cem alelos do gene Cystic Fibrosis Transmembrane Conductance Regulator, as mutações mais prevalentes foram Delta F508 (45%) e S4X (18%). Os grupos de mutações apresentaram associação somente (p=0,013) com a insuficiência pancreática e não com a gravidade da doença (p=0,073). Esta última apresentou associação com a colonização por Pseudomonas aeruginosa e Staphylococcus aureus (p=0,007) e com baixo peso (p=0,036). O óbito foi associado com a idade no diagnóstico (p=0,016), a sintomatologia respiratória (p=0,013), a colonização (p=0,024), o baixo peso (p=0,017) e a ocorrência de internação (p=0,003). Conclusões: Foi possível observar associação entre as mutações e a presença de insuficiência pancreática; entre a colonização por Staphylococcus aureus e o baixo peso com a gravidade da doença; e ausência de associação entre as mutações e a gravidade da doença. Os fatores ambientais merecem ser investigados mais detalhadamente, pois parecem apresentar impacto importante na gravidade da doença.

Abstract Objective: To analyze the association between phenotypic and genotypic characteristics and disease severity in individuals with cystic fibrosis treated at a reference center in Minas Gerais, Brazil. Methods: This is a retrospective study that collected clinical and laboratory data, respiratory and gastrointestinal manifestations, type of treatment, Shwachman-Kulczycki score, and mutations from the patients’ medical records. Results: The sample included 50 participants aged one to 33 years, 50% of whom were female. Out of the one hundred alleles of the Cystic Fibrosis Transmembrane Conductance Regulator gene, the most prevalent mutations were DeltaF508 (45%) and S4X (18%). Mutation groups were only associated with pancreatic insufficiency (p=0.013) and not with disease severity (p=0.073). The latter presented an association with colonization by Pseudomonas aeruginosa and Staphylococcus aureus (p=0.007) and with underweight (p=0.036). Death was associated with age at diagnosis (p=0.016), respiratory symptomatology (p=0.013), colonization (p=0.024), underweight (p=0.017), and hospitalization (p=0.003). Conclusions: We could identify the association of mutations with pancreatic insufficiency; the association of Staphylococcus aureus colonization and underweight with disease severity; and the lack of association between mutations and disease severity. Environmental factors should be investigated more thoroughly since they seem to have an important effect on disease severity.

AIM: To evaluate and compare the perception of parents of children with and without disabilities about the occurrence of local and systemic manifestations during the eruption of primary teeth and to investigate the parents' practices used to alleviate teething disturbances. METHODS: A cross-sectional study was conducted in a sample of parents of children without disabilities (GI) and parents of children with disabilities (GII) treated at a University Pediatric Dentistry Clinic. Data from GI and GII were collected using a structured questionnaire applied during an interview with the parents, and were analyzed using descriptive statistics. Mann-Whitney and Wilcoxon tests were employed and the level of significance was set at p<0.05. RESULTS: The questionnaires were filled out by 86 parents, being 45 in GI and 41 in GII. For GI, the most frequent local manifestations were edema around the tooth (84.44%) and increased suction (75.56%); and for GII were edema and erythema around the tooth (78.05% and 70.73%, respectively). The most frequent systemic manifestations, according to parents of both groups, were irritability and fever. There was no statistically significant difference between groups (p<0.05). It was observed that 46.66% and 68.3% of parents of GI and GII, respectively, adopted different practices to alleviate teething disturbances. CONCLUSIONS: During primary teeth eruption, local and systemic manifestations may occur and different practices were adopted by the Interviewed parents, mainly those with children with disabilities.

A microinfiltração marginal de restaurações de amálgama classe II (Dispersalloy), associada ao verniz convencional (Copalite) e a dois adesivos dentinários (Scotchbond Multi-uso Plus e Multi Bond Alpha), foi avaliada e comparada in vitro através de dois métodos: escores e medidas lineares. Foram utilizados 45 pré-molares hígidos que receberam duas cavidades independentes M e D. Após a restauração, foram submetidos à termociclagem e armazenados em uma solução de fucsina básica a 0,5% durante 24 horas. A análise da infiltração do corante permitiu concluir que nenhum dos três sistemas restauradores foi capaz de eliminar a microinfiltração marginal. No entanto, ela foi menor e estatisticamente significante para as restaurações associadas aos adesivos dentinários, quando comparadas àquelas com o verniz cavitário. O método das medidas lineares foi mais sensível do que o de escores.

The marginal microleakage of class II amalgam restorations (Dispersalloy) associated with copal varnish (Copalite) and with two dentin bonding agents (Scotchbond Multi-uso Plus and Multi Bond Alpha) was evaluated in vitro and compared by two methods: scores and linear measurements. Forty-five sound premolars were used, on which two separated class II cavities were prepared on the M and D surfaces. After the restoration, the specimens were thermocycled and stored in a solution of 0.5% basic fuchsin during 24 hours. The analysis allowed to conclude that none of the three restorative systems were able to eliminate the marginal microleakage. Nevertheless, the leakage was significantly smaller on the restorations associated with dentin bonding agents when compared to copal varnish. The linear measurement method was more sensitive than the score criteria.