RESUMO: A espécie Leopardus pardalis, conhecida popularmente como jaguatirica, é um felino de médio porte com ampla distribuição em território nacional e está incluída na lista de espécies da fauna brasileira ameaçadas de extinção. Este estudo teve como objetivo caracterizar os aspectos macroscópicos e microscópico da traqueia e lobos pulmonares em L. pardalis. As análises foram feitas utilizando cinco exemplares de jaguatirica, sendo dois machos e três fêmeas, adultos, três desses exemplares foram a óbito por atropelamento em rodovias próximas ao município de Alta Floresta, e dois doados após a morte pelo IBAMA ao o Laboratório de Zoologia e Morfologia Animal. Nos espécimes a traqueia era um tubo flexível formado por pares de arcos cartilaginosos, apresentando epitélio pseudo-estratificado não ciliado no seu revestimento interno, e outros estruturas básicas deste órgão. O pulmão se mostrou como um órgão constituído por lobos separados por fissuras com um parênquima usual aos demais mamíferos descritos. No que diz respeito às caraterísticas anatômicas da traqueia e pulmão se mostraram semelhantes à de outros mamíferos, já nos aspectos microscópicos da traqueia apresentou certas peculiaridades diferindo dos demais trabalhos encontrados. No que tange o aspecto do parênquima pulmonar as características histológicas não variaram com as que estão descritas.

ABSTRACT: The species Leopardus pardalis, popularly known as ocelot, is a medium sized cat with wide distribution in the national territory and is included in the list of species of Brazilian fauna threatened with extinction. This study aimed to characterize the macroscopic and microscopic aspects of the trachea and lung lobes in L. pardalis. Analyses were performed using five copies of ocelot, two males and three females, adults, three of these specimens died from being run over on highways nearby the municipality of Alta Floresta, and two donated after death by IBAMA to the Laboratory of Zoology and Animal Morphology. In the trachea specimens was a flexible tube formed by pairs of cartilaginous arches, with pseudo-stratified epithelium ciliated not in its inner lining, and other basic structures of this body. Lung proved as a body made up of lobes separated by cracks with a usual parenchyma to the other described mammals. With respect to the anatomic features of the trachea and lung were similar to other mammals, as in the microscopic aspects of the trachea showed certain peculiarities found differing from the other jobs. Regarding the appearance of the lung parenchyma histological characteristics did not differ with those described.

Several studies of the physiological responses of different organisms exposed to extremely low-frequency electromagnetic fields (ELF-EMF) have been described. In this work, we report the minimal effects of in situ exposure to ELF-EMF on the global protein expression of Chromobacterium violaceum using a gel-based proteomic approach. The protein expression profile was only slightly altered, with five differentially expressed proteins detected in the exposed cultures; two of these proteins (DNA-binding stress protein, Dps, and alcohol dehydrogenase) were identified by MS/MS. The enhanced expression of Dps possibly helped to prevent physical damage to DNA. Although small, the changes in protein expression observed here were probably beneficial in helping the bacteria to adapt to the stress generated by the electromagnetic field.

Este trabalho foi conduzido para estudar a distribuição de Cu, Fe, Mn e Zn nas frações proteicas de amostras de trigo submetidas a um procedimento de extração sequencial. Maiores concentrações dos elementos foram determinadas na fração extraída com tampão Tris-HCl contendo 1% de dodecil sulfato de sódio (SDS). As frações da amostra de trigo obtidas após o procedimento de extração foram analisadas por eletroforese em gel de poliacrilamida unidimensional (1D SDS-PAGE) e as bandas de proteínas foram tratadas para quantificar os metais por espectrometria de absorção atômica em forno de grafite (GF AAS). Desta forma, foi possível associar os metais Cu, Fe, Mn e Zn às bandas proteicas de peso molecular específico. As frações proteicas da amostra de trigo foram analisadas por espectrometria de massas por tempo de vôo acoplada à ionização dessortiva de matriz por laser (MALDI-TOF-MS), após procedimento de digestão proteica, identificando-se as proteínas lipid transfer e grain softness, sendo esta última possivelmente associada ao metal manganês.

This work was initially conducted to study the distribution of Cu, Fe, Mn and Zn in the protein fractions of wheat sample subjected to sequential extraction procedure. The highest metal concentrations were determined in the fraction extracted with Tris-HCl buffer containing 1% sodium dodecyl sulfate (SDS). The fractions of wheat sample obtained after the extraction procedure were analyzed by one-dimensional polyacrylamide gel electrophoresis (1D SDS-PAGE) and protein bands were treated to quantify Cu, Fe, Mn, and Zn by graphite furnace atomic absorption spectrometry (GF AAS). Metals were associated to protein bands of specific molecular weight, determined using a standard protein. The protein fractions were also analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) after a typical protein digestion protocol, identifying lipid transfer protein and grain softness, the latter being possibly associated to Mn.

A sequential extraction procedure was applied to wheat and soybean seed samples. The total protein content (determined by two distinct methods: Bradford and bicinchoninic acid-BCA) and distribution of Cu, Fe, Mn and Zn in each fraction was determined. The sequential extraction employed four different solutions: water, 0.5 mol L-1 NaCl, ethanol/water (70:30 v v-1) and 0.5 mol L-1 NaOH. For both samples, the highest concentration of metals was observed in those extracts associated with globulin-type proteins using NaCl solution. Regarding protein content, higher levels were obtained using the BCA method.

Uma nova substância, denominada habenariosídeo {[(2R)-2-[(2,3,4,6-tetra-O-acetil-β-D-glicopiranosil)oxi]-2-(2-metilpropil)-1,4-dioxo-1,4-butanodiil]bis(oximetileno-4,1-fenileno) bis-β-D-glicopiranosídeo}, foi isolada das frações polares do extrato etanólico de Habenaria petalodes Lindl. (Orchidaceae), juntamente com duas substâncias conhecidas e estruturalmente relacionadas, a loroglossina e a militarina. Os flavonóides: isoquercitrina, isorramnetina 3-O-β-D-glicopiranosídeo e isorramnetina 3,7-di-O-β-D-glicopiranosídeo, também foram isolados. As estruturas do habenariosídeo e das demais substâncias foram determinadas pela análise dos dados de EM, IV, UV e RMN mono e bidimensional e comparação com os dados publicados na literatura.

Habenarioside, a new natural product identified as [(2R)-2-[(2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl)oxy]-2-(2-methylpropyl)-1,4-dioxo-1,4-butanediyl]bis(oxymethylene-4,1-phenylene) bis-β-D-glucopyranoside, along with two known related metabolites, loroglossin and militarin, were isolated from the ethanol extract of the whole plant Habenaria petalodes Lindl. (Orchidaceae). The flavonoids isoquercitrin, isorhamnetin 3-O-β-D-glucopyranoside, and isorhamnetin 3,7-di-O-β-D-glucopyranoside were also isolated. The structures of all compounds were established by analysis of their MS, IR, UV, 1D and 2D NMR spectra, and comparison with published data.