Abstract Rimapenaeus constrictus is a penaeid shrimp widely distributed in the Western Atlantic, frequently captured as bycatch in trawling activities. Here we describe the weight vs. carapace length relationship and the condition factor of the species. Shrimps were sampled in the Ubatuba region, northern littoral of São Paulo State, monthly. We analyzed 4,952 individuals (1,371 males and 3,581 females). We measured the individuals’ weight and carapace length, and the condition factor (CF) was calculated for both sexes. Females had a heavier body when compared to males, probably due to their greater maximum body size achieved. Both sexes presented a negative allometric growth in weight, probably due to their reproductive pattern and activities. We found similar mean CF values for males and females. From temporal analysis, the highest CF values for females were observed during the seasons with lower water temperatures. Such a situation may happen because females’ CF tend to be influenced by a greater food availability in the environment, induced by the intrusion of the South Atlantic Central Water during the spring and early summer in the Ubatuba. The information presented here could be used as subside in protection actions and management of bycatch species.

O objetivo deste estudo foi verificar a relação entre a aptidão física e os indicadores de qualidade de vida de indivíduos com claudicação intermitente (CI). Participaram do estudo 42 indivíduos (65,2 ± 8,3 anos) com CI, de ambos os gêneros. Para a avaliação da aptidão física, foram realizados teste de esforço em esteira, para obtenção da distância de claudicação (DC) e da distância total de marcha (DTM) e o teste de uma repetição máxima no exercício extensão de joelho. Os indicadores de qualidade de vida foram avaliados por meio do Medical Outcome Study Questionnaire Short Form 36, composto por oito domínios: capacidade funcional (CF), aspectos físicos (AF), dor, estado geral de saúde, vitalidade (VI), aspectos sociais (AS), aspectos emocionais (AE) e saúde mental. Para a análise estatística foi utilizado o coeficiente de correlação de Pearson, com p < 0,05. Foi observada correlação significante entre o domínio da CF com a DC e a DTM (r = 0,60, p < 0,01 e r = 0,49, p < 0,01, respectivamente), o domínio da AF com a DTM (r = 0,46, p < 0,01), o domínio da VI e dos AS com a DC (r = 0,34, p = 0,03 e r = 0,33, p = 0,04, respectivamente) e o domínio da AE com a DC e a DTM (r = 0,43, p = 0,01 e r = 0,44, p = 0,01, respectivamente). Como conclusão, os resultados deste estudo sugerem que os indicadores da qualidade de vida nos domínios da saúde física e da saúde emocional são relacionados com a capacidade de caminhada de indivíduos com CI

The aim of this study was to investigate the relationship between physical fitness and the indicators of quality of life in individuals with intermittent claudication (IC). Forty-two subjects (65.2 ± 8.3 years) with IC of both genders participated in the study. Exercise treadmill test, to assess claudication distance (CD) and total walking distance (TWD), and one repetition maximum knee extension test were used to evaluate physical fitness. The quality of life indicators were obtained from the Medical Outcome Study Questionnaire Short Form, which is composed of eight domains: physical functioning (PF), physical aspects (PA), pain, general health, vitality (VI), social functioning (SF), emotional aspects (EA) and mental health. For statistical analysis Pearson correlation coefficient was used, with p < 0.05. There was a significant correlation between PF and CD and TWD (r = 0.60, p < 0.01 and r = 0.49; p < 0.01, respectively), between RP and TWD (r = 0.46, p < 0.01), between VI and SF and CD (r = 0.34, p = 0.03 e r = 0.33, p = 0.04; respectively), and between EA and CD and TWD (r = 0.43, p = 0.01 and r = 0.44, p = 0.01; respectively). In conclusion, the results of this study suggest that indicators of quality of life, both related to physical health and emotional health, are correlated with the walking capacity in patients with IC

This experiment was designed with the objective of developing a simple, practical, and high repeatability technique for the simultaneous evaluation of the integrity of the plasmatic and acrosomal membranes, as well as funcional mitochondria of domestic fowl spermatozoa using an association of fluorescent probes. Four ejaculates (motility > 80% and abnormal morphology < 10%) from each of six Ross male broiler breeder (n=24) were diluted in TALP sperm medium (25x10(6) spermatozoa/mL) and split into two aliquots, and one of these aliquots was flash frozen in liquid nitrogen and thawed to damage all cellular membranes. Three treatments were prepared from these aliquots, with the following ratios of Fresh semen:Flash frozen semen: 100:0 (T100), 50:50 (T50), and 0:100 (T0). A 150-µL aliquot of diluted semen was placed in a microcentrifuge tube with the addition of 2-µL PI, 2-µL MITO, and 50-µL FITC-PSA, and incubated at 38.5º C/8 min in the dark. An 8-µL sample was placed on a slide, coverslipped, and examined by epifluorescence microscopy. Each sample had 200 cells counted and classified based on the fluorescence emitted by each probe. By regression analysis, plasma membrane integrity, as detected by PI, was determined as: v=4.17+0.82X (R²=0.95). Acrosome integrity, as detected by FITC-PSA, generated the equation: v=4.19+0.84X (R²=0.96). Functional mitochondria was estimated by the equation v=3.20+0.83X (R²=0.96). This is an efficient technique to simultaneously evaluate plasmatic, acrosomal, and mitochondrial membranes in fowl sperm. It is suggested that its application in flow cytometry systems allows this methodology to be applied in large scale.