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[SciELO Preprints] - Guidelines on the Diagnosis and Treatment of Hypertrophic Cardiomyopathy – 2024
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Fernandes, Fabio Simões, Marcus V. Correia, Edileide de Barros Marcondes-Braga, Fabiana G. Coelho-Filho, Otavio Rizzi Mesquita, Cláudio Tinoco Mathias-Junior, Wilson Rochitte, Carlos Eduardo Ramires, Felix José Alvarez Alves, Silvia Marinho Martins Montera, Marcelo Westerlund Lopes, Renato Delascio Oliveira-Junior, Mucio Tavares Scolari, Fernando L. Avila, Walkiria Samuel Canesin, Manoel Fernandes Bacal, Fernando Bocchi, Edimar Alcides Moura, Lídia Ana Zytynski Saad, Eduardo Benchimol Scanavacca, Mauricio I. Valdigem, Bruno Pereira Cano , Manuel Nicolas Abizaid , Alexandre Ribeiro, Henrique Barbosa Lemos-Neto, Pedro Alves Ribeiro, Gustavo Calado de Aguiar Jatene, Fabio Biscegli Dias, Ricardo Ribeiro Beck-da-Silva, Luis Rohde, Luis Eduardo P. Bittencourt, Marcelo Imbroinise Pereira, Alexandre Krieger, José Eduardo Villacorta, Humberto Martins, Wolney de Andrade Figueiredo-Neto, José Albuquerque de Cardoso , Juliano Novaes Pastore, Carlos Alberto Jatene, Ieda Biscegli Tanaka, Ana Cristina Sayuri Hotta, Viviane Tiemi Romano, Minna Moreira Dias Albuquerque, Denilson Campos de Mourilhe-Rocha, Ricardo Hajjar, Ludhmila Abrahão Brito, Fabio Sandoli de Caramelli , Bruno Calderaro, Daniela Farsky, Pedro Silvio Colafranceschi , Alexandre Siciliano Pinto, Ibraim Masciarelli Vieira , Marcelo Luiz Campos Danzmann, Luiz Claudio Barberato , Silvio Henrique Mady, Charles Martinelli-Filho, Martino Torbey , Ana Flavia Malheiros Schwartzmann, Pedro Vellosa Macedo, Ariane Vieira Scarlatelli Ferreira , Silvia Moreira Ayub Schmidt, Andre Melo , Marcelo Dantas Tavares de Lima-Filho, Moysés Oliveira Sposito, Andrei C. Brito, Flavio de Souza Biolo, Andreia Madrini-Junior, Vagner Rizk, Stéphanie Itala Mesquita, Evandro Tinoco

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Fecha de publicación: 2024-04-08

Resumen: Pt Es En

A cardiomiopatia hipertrófica (CMH) é uma forma de doença do músculo cardíaco de causa genética, caracterizada pela hipertrofia das paredes ventriculares. O diagnóstico requer detecção por métodos de imagem (Ecocardiograma ou Ressonância Magnética Cardíaca) de qualquer segmento da parede do ventrículo esquerdo com espessura > 15 mm, sem outra causa provável. A análise genética permite identificar mutações de genes codificantes de diferentes estruturas do sarcômero responsáveis pelo desenvolvimento da CMH em cerca de 60% dos casos, permitindo o rastreio de familiares e aconselhamento genético, como parte importante do manejo dos pacientes e familiares. Vários conceitos sobre a CMH foram recentemente revistos, incluindo sua prevalência de 1 em 250 indivíduos, não sendo, portanto, uma doença rara, mas subdiagnosticada. A vasta maioria dos pacientes é assintomática. Naqueles sintomáticos, a obstrução do trato de saída do ventrículo esquerdo (OTSVE) é o principal distúrbio responsável pelos sintomas, devendo-se investigar a sua presença em todos os casos. Naqueles em que o ecocardiograma em repouso ou com Manobra de Valsalva não detecta gradiente intraventricular significativo (> 30 mmHg), devem ser submetidos à ecocardiografia com esforço físico para detecção da OTSVE.   Pacientes com sintomas limitantes e grave OTSVE, refratários ao uso de betabloqueadores e verapamil, devem receber terapias de redução septal ou uso de novas drogas inibidoras da miosina cardíaca. Por fim, os pacientes adequadamente identificados com risco aumentado de morta súbita podem receber medida profilática com implante de cardiodesfibrilador implantável (CDI).

La miocardiopatía hipertrófica (MCH) es una forma de enfermedad cardíaca de origen genético, caracterizada por el engrosamiento de las paredes ventriculares. El diagnóstico requiere la detección mediante métodos de imagen (Ecocardiograma o Resonancia Magnética Cardíaca) que muestren algún segmento de la pared ventricular izquierda con un grosor > 15 mm, sin otra causa probable. El análisis genético permite identificar mutaciones en genes que codifican diferentes estructuras del sarcómero responsables del desarrollo de la MCH en aproximadamente el 60% de los casos, lo que permite el tamizaje de familiares y el asesoramiento genético, como parte importante del manejo de pacientes y familiares. Varios conceptos sobre la MCH han sido revisados recientemente, incluida su prevalencia de 1 entre 250 individuos, por lo tanto, no es una enfermedad rara, sino subdiagnosticada. La gran mayoría de los pacientes son asintomáticos. En los casos sintomáticos, la obstrucción del tracto de salida ventricular izquierdo (TSVI) es el trastorno principal responsable de los síntomas, y su presencia debe investigarse en todos los casos. En aquellos en los que el ecocardiograma en reposo o la maniobra de Valsalva no detecta un gradiente intraventricular significativo (> 30 mmHg), deben someterse a ecocardiografía de esfuerzo para detectar la obstrucción del TSVI. Los pacientes con síntomas limitantes y obstrucción grave del TSVI, refractarios al uso de betabloqueantes y verapamilo, deben recibir terapias de reducción septal o usar nuevos medicamentos inhibidores de la miosina cardíaca. Finalmente, los pacientes adecuadamente identificados con un riesgo aumentado de muerte súbita pueden recibir medidas profilácticas con el implante de un cardioversor-desfibrilador implantable (CDI).

Hypertrophic cardiomyopathy (HCM) is a form of genetically caused heart muscle disease, characterized by the thickening of the ventricular walls. Diagnosis requires detection through imaging methods (Echocardiogram or Cardiac Magnetic Resonance) showing any segment of the left ventricular wall with a thickness > 15 mm, without any other probable cause. Genetic analysis allows the identification of mutations in genes encoding different structures of the sarcomere responsible for the development of HCM in about 60% of cases, enabling screening of family members and genetic counseling, as an important part of patient and family management. Several concepts about HCM have recently been reviewed, including its prevalence of 1 in 250 individuals, hence not a rare but rather underdiagnosed disease. The vast majority of patients are asymptomatic. In symptomatic cases, obstruction of the left ventricular outflow tract (LVOT) is the primary disorder responsible for symptoms, and its presence should be investigated in all cases. In those where resting echocardiogram or Valsalva maneuver does not detect significant intraventricular gradient (> 30 mmHg), they should undergo stress echocardiography to detect LVOT obstruction. Patients with limiting symptoms and severe LVOT obstruction, refractory to beta-blockers and verapamil, should receive septal reduction therapies or use new drugs inhibiting cardiac myosin. Finally, appropriately identified patients at increased risk of sudden death may receive prophylactic measure with implantable cardioverter-defibrillator (ICD) implantation.

https://doi.org/10.1590/SciELOPreprints.8394

Light intensities alter growth and essential oil of patchouli under shade nets
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Ribeiro, Aurislaine Santos

; Bertolucci, Suzan Kelly Vilela

; Carvalho, Alexandre Alves de

; Tostes, Wesley Naves

; Coelho, Adriane Duarte

; Pinto, José Eduardo Brasil Pereira

Ciência Rural

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2022, Volumen 52 Nº 5 elocation e20210118

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: Pogostemon cablin (Blanco) Benth. é uma espécie aromática popularmente conhecida como patchouli. O óleo essencial rico em patchoulol extraído de folhas é utilizado pelas indústrias farmacêuticas. O objetivo foi investigar os efeitos das malhas e intensidades de sombreamento no crescimento, anatomia, composição química e teor de óleo essencial de P. cablin. O experimento foi conduzido com dois tipos de malhas (preta e Aluminet), três intensidades de luz (30, 50 e 70% de sombreamento) e pleno sol. As diferentes intensidades de luz influenciaram o crescimento, o rendimento e a composição química do óleo essencial. Plantas de patchouli cultivadas em ambiente sombreado apresentaram maiores valores para os pesos secos de folha, caule, raiz e total em relação ao pleno sol. Com 50% de sombreamento, Aluminet proporcionou ganho de peso seco de caule e total. Aluminet e malha preta a 50 e 70% de sombreamento aumentam o rendimento do óleo essencial. Os teores de patchoulol e pogostol foram maiores a pleno sol, malha preta e Aluminet com 50% de sombreamento. O ambiente para o cultivo do patchouli pode ser melhorado com o uso de malhas de sombreamento, especialmente com Aluminet em 50% de sombreamento.

ABSTRACT: Pogostemon cablin (Blanco) Benth. is an aromatic species popularly known as patchouli. The essential oil rich in patchoulol extracted from leaves is used by the pharmaceutical industries. The objective was to investigate the effects of shade nets and shading intensities on P. cablin growth, anatomy, chemical composition and essential oil content. The experiment was conducted with two types of shade nets (black and Aluminet), three light intensities (30, 50, and 70% shading) and full sun. The different light intensities influenced the growth and the essential oil yield, and chemical composition. Patchouli plants grown in an environment with shading showed higher values for the leaf, stem, root ant total dry weights compared to full sun. At 50% of shading, Aluminet provided gain in stem and total dry weights. Aluminet and black net at 50 and 70% of shading enhance essential oil yield. Patchoulol and pogostol contents were higher under full sun, and, black net and Aluminet at 50% shading. The environment for patchouli cultivation can be improved by use shade nets, especially with Aluminet at 50% of shading.

https://doi.org/10.1590/0103-8478cr20210118 178 descargas

Potassium uptake kinetics in native forage grass species from Pampa Biome
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Fernandes, Gracieli

; Marques, Anderson César Ramos

; Ribeiro, Bruna San Martin Rolim

; Cardoso, Paula de Souza

; Tagliapietra, Eduardo Lago

; Friedrich, Eduardo Daniel

; Weber, Patric Scolari

; Krug, Amanda Veridiana

; Kulmann, Matheus Severo de Souza

; Paula, Betania Vahl de

; Nicoloso, Fernando Teixeira

; Brunetto, Gustavo

Ciência Rural

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2022, Volumen 52 Nº 11 elocation e20200708

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: O estudo quantificou os parâmetros cinéticos de captação de K nas gramíneas Paspalum notatum, Paspalum urvillei e Axonopus affinis associados com o crescimento. Dez mililitros (10 ml) de solução nutritiva foram coletados em vasos de dois litros - com cinco repetições em 24 horas - para determinação das concentrações de K nas amostras. A matéria seca da parte aérea e raízes; comprimento, volume, diâmetro e área superficial radicular, bem como parâmetros cinéticos associados à absorção de nutrientes (Vmax, Km, Cmin e I) foram avaliados. A espécie P. notatum apresentou a maior produção de raiz, parte aérea e MS total, bem como o maior volume de raiz. P. urvillei apresentou o maior teor de K, que pode ser associado a raízes mais finas e resultando em maior área superficial. A. affinis registrou o maior valor de Vmax, o que levou a uma maior eficiência de absorção de K.

ABSTRACT: This study quantified K uptake kinetic parameters in grass species Paspalum notatum, Paspalum urvillei and Axonopus affinis to associate them with growth. Ten milliliters (10 ml) of nutrient solution were collected in two-liter pots - with five repetitions in 24 hours - in order to determine K concentrations in the samples. Shoot and root dry matter, root length, volume, diameter and surface area, as well as kinetic parameters associated with nutrient uptake (Vmax, Km, Cmin and I) were determined. Species P. notatum was the one presenting the highest root, shoot and total dry matter production, as well as the highest root volume. P. urvillei recorded the highest K content, which may be associated with thinner roots and greater root surface area. A. affinis recorded the highest Vmax value, which led to greater K uptake efficiency.

https://doi.org/10.1590/0103-8478cr2020

Triatomine fauna in the state of Bahia, Brazil: What changed after 40 years of the vector-control program?
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Ribeiro-Jr, Gilmar

; Araújo, Renato Freitas de

; Carvalho, Cristiane Medeiros Moraes de

; Cunha, Gabriel Muricy

; Lanza, Fernanda Cardoso

; Miranda, Diego Lopes Paim

; Sousa, Orlando Marcos Farias de

; Santos, Carlos Gustavo Silva dos

; Fonseca, Eduardo Oyama Lins

; Santos, Roberto Fonseca dos

; Reis, Renato Barbosa

; Gurgel-Gonçalves, Rodrigo

; Reis, Mitermayer Galvão

Revista da Sociedade Brasileira de Medicina Tropical

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2022, Volumen 55 elocation e0732-2021

Resumen: En | Texto: En | PDF: En

ABSTRACT Background: Neglected tropical diseases are a growing threat to global health, and endemic Chagas disease has emerged as one of the most important health problems in America. The main strategy to prevent Trypanosoma cruzi transmission is chemical control of vectors. This study presents a descriptive analysis of synanthropic triatomines before and after the implementation of a vector-control program in Bahia, Brazil. Methods: Descriptive analysis and geospatial statistics were performed on triatomine data, (1) the relative abundance and (2) proportional spatial distribution, from Bahia during two periods: (A) 1957 to 1971 and (B) 2006 to 2019. Results: We observed a decrease in the relative abundance of Panstrongylus megistus (A: n=22.032, 61.9%; B: n=1.842, 1.0%) and Triatoma infestans (A: n=1.310, 3.7%; B: n=763, 0.43%), as well as an increase in the relative abundance of T. sordida (A: n=8.314, 23.4%, B: n=146.901, 81.6%) and T. pseudomaculata (A: n=894, 2.5%, B: n=16.717, 9.3%). Conclusions: Our results indicate a clear reduction in the occurrence of P. megistus and T. infestans (last record in 2015) and an increase in the relative abundance and geographical distribution of T. sordida and T. pseudomaculata after 40 years of the vector-control program. The high frequency of other triatomine species in the municipalities of the state of Bahia and their abundance in recent years highlight the need to reinforce permanent entomological surveillance actions to prevent Chagas disease.

https://doi.org/10.1590/0037-8682-0732-2021

Estimating the light conversion efficiency by sugarcane: the segmented approach
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CRUZ, LARISSA P.

; MACHADO, EDUARDO C.

; RIBEIRO, RAFAEL V.

Anais da Academia Brasileira de Ciências

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2022, Volumen 94 Nº 2 elocation e20211317

Resumen: En | Texto: En | PDF: En

Abstract The classical method to estimate the light conversion efficiency (εc) gives a single value for the whole crop cycle (εco) but does not reveal any variation along the growing season. We proposed the segmented approach to uncover such variations along sugarcane (Saccharum sp. hybrid) growth cycle. Our analyses revealed that longer sampling intervals could overestimate εco and that the segmented light conversion efficiency (εcs) varied between 0.09 and 5.39 g MJ-1 during the crop cycle. The εcs would provide insights on how the environment affects εc and how to increase biomass production through crop management practices.

https://doi.org/10.1590/0001-3765202220211317

Quality of life in enuretic children
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Rangel, Raquel A.

; Seabra, Carolina Ribeiro

; Ferrarez, Carlos Eduardo P. F.

; Soares, Josana L.

; Choi, Mauro

; Cotta, Robert Gomes

; Figueiredo, Andre Avarese de

; Bessa Jr., José de

; Murillo B. Netto, Jose

International braz j urol

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Jun 2021, Volumen 47 Nº 3 Paginas 535 - 541

Resumen: En | Texto: En | PDF: En

ABSTRACT Introduction: Nocturnal enuresis is a highly incident chronic disorder that generates countless problems to the child and their parents. Bed-wetting has significant negative impacts on self-esteem and the performance of children. The aim of the current study is to assess the quality of life of enuretic children, as well as its association to sex and age. Patients and Methods: Thirty-nine enuretic children (23 boys) and 49 healthy children (27 boys) without any history of previous treatment for enuresis or voiding dysfunction were included. Age ranged between 6 and 11 years old. The “AUQEI” questionnaire was applied in a private environment to all children by the same researcher (psychologist) to evaluate quality of life. Results: Enuretic children displayed loss in quality of life when compared to non-enuretic (35.9% of enuretic x 16.3% of non-enuretic, p=0.035). They were mostly affected in their daily activities (p=0.02). No significant differences were found in the association of sex and gender with quality of life. These results suggest that, children with nocturnal enuresis have 2.87 times more chances of having loss in quality of life compared to non-enuretic. Conclusions: Enuresis has a great impact in quality of life of children. This impact is not related to the age or sex of the child.

https://doi.org/10.1590/s1677-5538.ibju.2020.0308 367 descargas

Length-weight relationship and diet of the catfish Cambeva guareiensis (Siluriformes; Trichomycteridae)
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Ribeiro, Felipe H. N. P.

; Bonan, Maria E. P.

; Silva, José Lucas Dias da

; Azevedo-Santos, Valter M.

; Manoel, Pedro S.

; Brambilla, Eduardo M.

; Silva, Carolina V.

Biota Neotropica

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2021, Volumen 21 Nº 4 elocation e20211236

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo: A relação peso-comprimento (RPC) e dieta de um pequeno bagre trichomycterídeo, Cambeva guareiensis, foi estudada com base em 58 espécimes capturados no riacho Corrente, bacia do rio Guareí, São Paulo, Brasil. As análises de RPC mostraram valores de a variando entre 0,0097-0,0149 e de b entre 2,858-3,250. A análise da dieta mostrou que altas quantidades de insetos aquáticos (93,75% do total de itens) foram consumidas pela espécie. Além disso, não encontramos diferenças estatisticamente significativas quando comparamos a dieta entre os períodos seco e chuvoso. Nosso estudo contribui para o conhecimento da biologia populacional e ecologia alimentar de peixes trichomicterídeos em riachos brasileiros.

Abstract: The length-weight relationship (LWR) and diet of a small trichomycterid catfish, Cambeva guareiensis, was studied based on 58 specimens captured in Corrente stream, Guareí River basin, São Paulo, Brazil. LWR analyses showed values of a ranged from 0.0097 to 0.0149 and b from 2.858 to 3.250. The analysis of the diet showed that high amounts of aquatic insects (93.75% of total items) were consumed by the species. In addition, we found no statistically significant differences when comparing the diet between the dry and rainy periods. Our study contributes to the knowledge on populational biology and feeding ecology of trichomycterid fishes in Brazilian streams.

https://doi.org/10.1590/1676-0611-bn-2021-1236 173 descargas

From Spanish Flu to Syndemic COVID-19: long-standing sanitarian vulnerability of Manaus, warnings from the Brazilian rainforest gateway
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RIBEIRO, SÉRVIO P.

; REIS, ALEXANDRE B.

; DÁTTILO, WESLEY

; SILVA, ALCIDES V.C. DE CASTRO E

; BARBOSA, EDUARDO AUGUSTO G.

; COURA-VITAL, WENDEL

; GÓES-NETO, ARISTÓTELES

; AZEVEDO, VASCO A.C.

; FERNANDES, GERALDO WILSON

Anais da Academia Brasileira de Ciências

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2021, Volumen 93 elocation e20210431

Resumen: En | Texto: En | PDF: En

Abstract A second deadlier wave of COVID-19 and the causes of the recent public health collapse of Manaus are compared with the Spanish flu events in that city, and Brazil. Historic sanitarian problems, and its hub position in the Brazilian airway network are combined drivers of deadly events related to COVID-19. These drivers were amplified by misleading governance, highly transmissible variants, and relaxation of social distancing. Several of these same factors may also have contributed to the dramatically severe outbreak of H1N1 in 1918, which caused the death of 10% of the population in seven months. We modelled Manaus parameters for the present pandemic and confirmed that lack of a proper social distancing might select the most transmissible variants. We succeeded to reproduce a first severe wave followed by a second stronger wave. The model also predicted that outbreaks may last for up to five and half years, slowing down gradually before the disease disappear. We validated the model by adjusting it to the Spanish Flu data for the city, and confirmed the pattern experienced by that time, of a first stronger wave in October-November 1918, followed by a second less intense wave in February-March 1919.

https://doi.org/10.1590/0001-3765202120210431 337 descargas

Two Years Monitoring of Ethyl Carbamate in Sugar Cane Spirit from Brazilian Distilleries
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Bueno, Rafaela C.

; Tonin, Angélica P. P.

; Poliseli, Camila B.

; Sinosaki, Nayane B. M.

; Oliveira, Claudio C.

; Visentainer, Jesuí V.

; Ribeiro, Marcos A. S.

; Silva, Valquíria M.

; Meurer, Eduardo C.

Journal of the Brazilian Chemical Society

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Jul 2020, Volumen 31 Nº 7 Paginas 1461 - 1466

Resumen: En | Texto: En | PDF: En

This paper describes the monitoring of ethyl carbamate concentration in sugar cane spirit from industrial distilleries in Brazil. In total, 18 distilleries from Minas Gerais and São Paulo states were evaluated, with, a total of 336 samples from August 2017 to August 2019. The ethyl carbamate was analyzed using a previously validated mass spectrometric method. Analytical curves presented coefficient of determination (R2) values higher than 0.99, and quality control samples showed a relative standard deviation (RSD) below 9%. Concentrations of ethyl carbamate ranged from not detected to 1608 µg L-1, with mean values of 294.2, 280.7 and 230.0 µg L-1 in 2017, 2018, and 2019, respectively. On average, 46, 44 and 69% of samples presented levels of ethyl carbamate below 210 µg L-1. A variety of factors may have led to distillery A having a low level (ca. 115 µg L-1) of ethyl carbamate, including controlled temperature, yeast strain, post-harvesting quality, sugar cane variety choice, and the treatment process on the sugar cane juice, which goes towards the addition of Ca(OH)2, heating and freezing. These results showed the importance of internal quality control, and that sugar cane juice treatment may be a way to decrease ethyl carbamate in the distilleries.

https://doi.org/10.21577/0103-5053.20200031 200 descargas

10.

Structural Study of Phenolic Acids by Triple Quadrupole Mass Spectrometry with Electrospray Ionization in Negative Mode and H/D Isotopic Exchange
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Sinosaki, Nayane B. M.

; Tonin, Angélica P. P.

; Ribeiro, Marcos A. S.

; Poliseli, Camila B.

; Roberto, Sharise B.

; Silveira, Roberta da

; Visentainer, Jesuí V.

; Santos, Oscar O.

; Meurer, Eduardo C.

Journal of the Brazilian Chemical Society

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Feb 2020, Volumen 31 Nº 2 Paginas 402 - 408

Resumen: En | Texto: En | PDF: En

This work reports the theoretical and experimental study of fragmentation reactions in the gas phase of five phenolic acids using triple quadrupole mass spectrometry by electrospray ionization in negative ionic mode, as well as the isotope exchange experiments. MS/MS spectra were analyzed to suggest the fragmentation mechanisms, while theoretical calculations at the theory level B3LYP/6-311+G** were performed to expose the proposed mechanisms viability for this class of compounds. As expected, compounds with aromatic methoxy substitution presented •CH3 radical elimination as the principal fragmentation pathway, forming dystonic ions. Compounds without methoxy substituents dissociate with higher energies losing the CO2, CO and H2O. The isotopic marking experiments indicated the exchange of hydrogens by deuterases in the hydroxyl protons, which corroborates with the proposed mechanisms.

https://doi.org/10.21577/0103-5053.20190197 1126 descargas

11.

Fast Triacylglycerol Screening and Semi-Quantitative Analysis of Fatty Acids in Commercial Oils by DIMS with 18-Crown-6 Ether/Trifluoracetic Acid Dopants
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Tonin, Angelica P. P.

; Ribeiro, Marcos A. S.

; Poliseli, Camila B.

; Silveira, Roberta da

; Visentainer, Jesuí V.

; Silva, Valquíria M.

; Ribeiro, Leomara F.

; Souza, Rodrigo C. T. de

; Oliveira, Cláudio C. de

; Meurer, Eduardo C.

Journal of the Brazilian Chemical Society

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Feb 2019, Volumen 30 Nº 2 Paginas 350 - 358

Resumen: En | Texto: En | PDF: En

Vegetable oil analyses, especially due to the complexity of the oil components, are commonly laborious, requiring several analytical techniques. In this work, electrospray ionization mass spectrometry with direct injection (DIMS), along with 18-crown-6 ether (crown ether) chelating agent and trifluoroacetic acid (TFA), was used to characterize and semi-quantitatively evaluate commercial vegetable oils. As a result, an unprecedented DIMS method of triacylglycerols (TAGs) analysis for semi-quantitative profiling of fatty acids in commercial oils (e.g., soybean, sunflower, corn, oil, canola), without sample derivatization, was developed. The results of the ion abundances related to the analyzed TAGs, with quantitative percentage analyzed by gas chromatography with flame ionization detector (GC-FID), allowed generating correction factors for each oil. DIMS analysis with crown ether/TFA resulted in the elimination of isobaric interferences from sodium and potassium adducts, facilitating the ion assignments, due to the one-ion-per-molecule observation in the mass spectrum. Chemometric analyses by principal component analysis (PCA) and heatmap, to evaluate the ionic profile of the oils, grouped them accordingly to their TAG content. Olive oil was identified as the most different from the other oils. Overall, a semi-quantitative approach to investigate the vegetable oils, reaching low percentage variation for DIMS, with 2 min analyses and 30 min sample preparation, was proposed.

https://doi.org/10.21577/0103-5053.20180184 631 descargas

12.

Growth and biochemical variables in Amazon catfish ( Pseudoplatystoma reticulatum ♀ x Leiarius marmoratus ♂) under different water pH
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LEMOS, CARLOS HENRIQUE P.

; CHUNG, SAMANTHA

; RIBEIRO, CLÁUDIO V.M.

; COPATTI, CARLOS EDUARDO

Anais da Academia Brasileira de Ciências

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Dic 2018, Volumen 90 Nº 4 Paginas 3573 - 3581

Resumen: En | Texto: En | PDF: En

Abstract Although fish may live in different aquatic environments, variations in the water quality parameters, mainly pH, may have a substantial impact in their development and welfare. The objective of this study was to test the effect of different pH levels on the growth performance and biochemical variables in Amazon catfish juveniles (Pseudoplatystoma reticulatum x Leiarius marmoratus). Fish were subjected to four different water pH levels (4.56, 6.0, 7.24, and 8.90) for 40 days. The total ammonia and alkalinity were higher and lower, respectively, in the treatments with water pHs 4.56 and 6.00 than at the other water pH levels. The un-ionized ammonia was higher at water pH 8.90 than at the other water pH levels. Amazon catfish exposed to water pH 4.56 presented specific growth rate and weight gain significantly higher than other water pH values. In general, fish under exposure to water pH 8.90 presented lower growth performance than other water pH values. Biochemical changes were recorded at all water pH. In conclusion, water pH 4.56 is recommended for an Amazon catfish production system, since fish presented higher growth and water quality variables were kept within adequate limits for aquaculture.

https://doi.org/10.1590/0001-3765201820180241 817 descargas

13.

Colored shade nets induced changes in growth, anatomy and essential oil of Pogostemon cablin
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RIBEIRO, AURISLAINE S.

; RIBEIRO, MARIANA S.

; BERTOLUCCI, SUZAN K.V.

; BITTENCOURT, WANDERLEY J.M.

; CARVALHO, ALEXANDRE A. DE

; TOSTES, WESLEY N.

; ALVES, EDUARDO

; PINTO, JOSÉ E.B.P.

Anais da Academia Brasileira de Ciências

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Abr 2018, Volumen 90 Nº 2 Paginas 1823 - 1835

Resumen: En | Texto: En | PDF: En

ABSTRACT The purpose of this investigation was to determine the influence of colored shade nets on the growth, anatomy and essential oil content, yield and chemical composition of Pogostemon cablin. The plants were cultivated under full sunlight, black, blue and red nets. The harvesting was performed 5 months after planting and it was followed by the analysis of plant growth parameters, leaf anatomy, essential oil content, yield and chemical composition. The plants grown under red net have produced more leaf, shoot, total dry weight and leaf area. Plants cultivated under colored nets showed differences in morphological features. Plants maintained under red net had a higher leaf blade thickness and polar and equatorial diameter of the stomata ratio. Additionally, higher yield of essential oil in the leaves was observed under red and blue colored shade net. The essential oil of the plants grown under red net showed the highest relative percentage of patchoulol (66.84%). Therefore, it is possible using colored shade nets to manipulate P. cablin growth, as well as its essential oil production with several chemical compositions. The analyses of principal components allowed observing that pogostol has negative correlation with α-guaiene and α-bulnesene. There was difference in total dry weight and patchoulol content when the patchouli is cultured under the red colored shade nets.

https://doi.org/10.1590/0001-3765201820170299 1131 descargas

14.

Marketable yield of onion under different irrigation depths, with and without mulch
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Carvalho, Daniel F. de

; Ribeiro, Eduardo C.

; Gomes, Daniela P.

Revista Brasileira de Engenharia Agrícola e Ambiental

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Feb 2018, Volumen 22 Nº 2 Paginas 107 - 112

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Objetivou-se neste estudo obter os coeficientes de cultivo para a cultura da cebola e avaliar a influência de diferentes lâminas de irrigação (0, 22, 45, 75 e 100% da evapotranspiração da cultura) na produtividade comercial e na qualidade de bulbos de cebola, cultivada com e sem cobertura vegetal morta de capim-elefante no solo. O experimento foi realizado em Seropédica, RJ, Brasil, entre maio e setembro de 2012, em um Argissolo Vermelho-Amarelo. O delineamento experimental adotado foi em blocos casualizados em parcelas subdivididas, com10 tratamentos e sete repetições. O manejo da irrigação foi realizado por meio do balanço de água no solo utilizando a técnica da Reflectometria no Domínio do Tempo, a partir de sondas instaladas horizontalmente a 7,5 e 22,5 cm de profundidade. A utilização da cobertura morta permitiu a aplicação de menores lâminas de irrigação, proporcionando coeficientes de cultivo inferiores (18% no estágio II e 3% no estágio III) ao cultivo sem cobertura do solo. A lâmina de irrigação associada ao uso da cobertura vegetal morta no solo influenciou as variáveis de produção avaliadas, demonstrando ser uma alternativa para elevar a produtividade comercial e a qualidade de bulbos de cebola com menor lâmina de irrigação.

ABSTRACT The objectives of this study were to obtain the onion crop coefficients and evaluate the influence of different irrigation depths (0, 22, 45, 75 and 100% of crop evapotranspiration) on marketable yield and quality of onion bulbs cultivated with and without mulch of elephant grass. The experiment was carried out in Seropédica, RJ, Brazil, from May to September 2012, in a Red Yellow Argisol. The experimental design was in randomized blocks in split plots, with 10 treatments and seven replicates. Irrigation management was performed through soil water balance using the Time Domain Reflectometry technique, with probes installed horizontally at 7.5 and 22.5 cm depths. The use of mulch allowed the application of smaller irrigation depths, leading to lower crop coefficient (18% in stage II and 3% in stage III) in comparison to the crop without mulch. Irrigation depths associated with the use of mulch influenced the evaluated production variables, proving to be an alternative to increase marketable yield and quality of onion bulbs, with lower irrigation depth.

https://doi.org/10.1590/1807-1929/agriambi.v22n2p107-112 1047 descargas

15.

EpiFibro (Brazilian Fibromyalgia Registry): data on the ACR classification and diagnostic preliminary criteria fulfillment and the follow-up evaluation
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Martinez, José Eduardo

; Paiva, Eduardo S.

; Rezende, Marcelo C.

; Heymann, Roberto E.

; Helfenstein Jr., Milton

; Ranzolin, Aline

; Provenza, Jose Roberto

; Ribeiro, Luiz Severiano

; Souza, Eduardo J.R.

; Feldman, Daniel P.

; Assis, Marcos Renato de

Revista Brasileira de Reumatologia

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Abr 2017, Volumen 57 Nº 2 Paginas 129 - 133

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Introdução: O EpiFibro (Estudo Epidemiológico Brasileiro de Fibromialgia) foi criado para estudar pacientes com fibromialgia. Foram incluídos pacientes desde 2011 de acordo com os critérios de classificação para a fibromialgia do American College of Rheumatology de 1990 (ACR1990). Objetivos: Determinar quantos pacientes ainda atendem aos critérios ACR1990 e ACR2010 em 2014; determinar a correlação entre o impacto da FM medido pelo Questionário de Impacto da Fibromialgia (FIQ) e pela Polysymptomatic Distress Scale (PDS) e descrever dados sobre a avaliação de seguimento. Métodos: Estudo transversal em uma coorte multicêntrica de pacientes. Os dados foram coletados entre 2013 e 2015. O médico incluiu pacientes que atenderam aos critérios ACR1990 no momento da entrada. Consideraram-se os dados de seguimento apenas dos pacientes com pelo menos duas avaliações. Uma variação de 30% nos escores dos parâmetros foi considerada uma alteração minimamente significativa. Resultados: Analisaram-se os dados de 810 pacientes. Os pacientes apresentaram média de 51,8 ± 11,5 anos. Havia 786 mulheres. A maior parte dos pacientes atendeu a ambos os critérios. Houve um maior atendimento aos critérios ACR2010. Houve uma correlação moderada entre a PDS e o FIQ. Encontraram-se 314 pacientes com mais de uma avaliação, mas 88 pacientes foram excluídos. Assim, foram considerados 226 pacientes com um parâmetro de monitoramento no seguimento. (FIQ: 222; PDS: 199; ambos: 195). O tempo médio de seguimento foi de 9,1 ± 7,5 meses (1 a 44). A maior parte dos pacientes tornou-se estável. Conclusão: No EpiFibro, a maior parte dos pacientes atendia simultaneamente ao ACR1990 e ao ACR2010. Uma maior quantidade de pacientes atendia ao ACR2010 no momento da avaliação. Houve uma correlação moderada. A maior parte dos pacientes manteve-se estável ao longo do tempo.

Abstract Introduction: EpiFibro (Brazilian Epidemiological Study of Fibromyalgia) was created to study Fibromyalgia patients. Patients were included since 2011 according to the 1990 American College of Rheumatology Classification Criteria for Fibromyalgia (ACR1990). Objectives: To determine how many patients still fulfill the ACR1990 and the ACR2010 criteria in 2014; to determine the correlation between the impact of FM and to describe data on the follow-up evaluation. Methods: This is a cross sectional study in a multicenter cohort of patients. The data was collected between 2013 and 2015. Physician included patients that fulfilled the ACR1990 criteria on the date of entry. The follow-up data were considered only for patients with at least two evaluations. A minimally significant change was considered to be a 30% variation of parameters scores. Results: 810 patients’ data were analyzed. Patients presented a mean age of 51.8 ± 11.5 years old. There were 786 female. Most patients met both criteria. There was a greater fulfilling of the ACR2010. There was a moderate correlation between Polysymptomatic Distress Scale and Fibromyalgia Impact Questionnaire. Three hundred fourteen patients with more than one assessment were found, but 88 patients were excluded. Thus, 226 patients with one follow-up monitoring parameter were considered (Fibromyalgia Impact Questionnaire: 222; Polysymptomatic Distress Scale: 199; both: 195). The mean follow-up time was 9.1 ± 7.5 months (1–44). Most patients became stable. Conclusion: InEpiFibro, most patients fulfill simultaneously the ACR1990 and ACR2010. A larger number of patients fulfill the ACR2010 at the time of the evaluation. There was a moderate correlation between the Polysymptomatic Distress Scale and the Fibromyalgia Impact Questionnaire. Most patients remained stable over time.

https://doi.org/10.1016/j.rbre.2016.09.012 8286 descargas

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