ABSTRACT Developing countries face great challenges to couple agricultural intensification and sustainable supply chain development. This paper explores the opportunities for innovation in the conservation of the locally adapted Curraleiro Pé-Duro (CPD) cattle. The CPD is a historical Brazilian breed, related to regional culture, which exhibits remarkable adaptability in tropical biomes. The research method consisted of interviewing and analyzing the discourse of CPD breeders in Tocantins, Brazil. The systematization of the data resulted in four categories and eleven subcategories, which were defined a posteriori. Our findings reveal motivational aspects, potentialities, and prospects for valuation, besides the needs and perceived challenges faced by CPD farmers. A better understanding of the current situation may guide public policies, encourage stakeholders, benefit farmers, and lead to sustainable agri-food initiatives. The empirical contributions of our diagnosis are discussed, and we show how they bring forward and provide valuable lessons to prospect innovations in the conservation of CPD cattle. These findings may support scholars and people who are also interested in accessing and supporting the conservation of indigenous livestock. In general, the study supports the definition of strategies, planning, and investments for the value chain, including the participation of upstream and downstream segments of the farms.

RESUMO Os países em desenvolvimento enfrentam grandes desafios para combinar a intensificação da agricultura com o desenvolvimento sustentável das cadeias de abastecimento. Este documento explora as oportunidades de inovação na conservação do gado Curraleiro Pé-Duro (CPD). O CPD é uma raça brasileira histórica, relacionada à cultura regional, que apresenta notável adaptabilidade em biomas tropicais. O método de pesquisa consistiu em entrevistar e analisar o discurso dos criadores de CPD no Tocantins, Brasil. A sistematização dos dados resultou em quatro categorias e onze subcategorias, que foram definidas a posteriori. Nossas descobertas revelam aspectos motivacionais, potencialidades e perspectivas de valorização, além das necessidades e desafios percebidos pelos produtores de CPD. Uma melhor compreensão da situação atual pode orientar as políticas públicas, incentivar os interessados, beneficiar os agricultores e levar a iniciativas agroalimentares sustentáveis. As contribuições empíricas de nosso diagnóstico são discutidas e mostramos como elas trazem e fornecem lições valiosas para prospectar inovações na conservação do gado de CPD. Estas descobertas podem apoiar acadêmicos e pessoas que também estão interessadas em acessar e apoiar a conservação e produção animal com uso de raças crioulas. Em geral, o estudo apoia a definição de estratégias, planejamento e investimentos relacionados com cadeia de valor, incluindo a participação de segmentos a montante e a jusante das fazendas.

ABSTRACT This study aimed to evaluate the effects of diets containing elephant grass genotypes silages as exclusive roughage on leg tissue composition, and physicochemical characteristics of meat of lambs. Twenty-four crossbred male lambs with an average initial body weight of 20.29±2.66kg were distributed in a complete randomized design with three treatments and eight replicates. The treatments consisted of three silages of elephant grass genotypes (IRI-381, Elephant B or Mott), without additives or wilting, as the only roughage. The diets did not affect (P>0.05) the dry matter (898.70±60.10 g/day), crude protein (128.93±6.91g/day), total digestible nutrients (690.20±91.82g/day) intakes, body weight at slaughter (24.83±2.79kg), and carcass yields (P>0.05). The tissue composition of the leg did not differ significantly between silages of elephant grass genotypes (P>0.05). No difference (P>0.05) for the physicochemical characteristics of meat from lambs fed diets tested was observed. Therefore, our results indicate that diets containing 50% elephant grass genotypes silages (IRI-381, Elephant B or Mott), harvested at 60 days of growth, have potential for use in lambs feeding.

RESUMO Este estudo teve como objetivo avaliar os efeitos de dietas contendo silagens de genótipos de capim-elefante como volumoso exclusivo sobre a composição tecidual da perna e nas características físico-químicas da carne de cordeiros. Vinte e quatro cordeiros machos mestiços, com peso corporal inicial médio de 20,29±2,66kg, foram distribuídos em delineamento inteiramente ao acaso, com três tratamentos e oito repetições. Os tratamentos consistiram de três silagens de genótipos de capim-elefante (IRI-381, Elephant B ou Mott), sem aditivos ou emurchecimento, como único volumoso. As dietas não afetaram (P>0,05) os consumos de matéria seca (898,70 ± 60,10g/dia), proteína bruta (128,93±6,91g/dia) e nutrientes digestíveis totais (690,20±91,82g/dia), peso corporal ao abate (24,83±2,79kg) e rendimentos de carcaça (P>0,05). A composição tecidual da perna não diferiu significativamente entre as silagens dos genótipos de capim-elefante (P>0,05). Não foi observada diferença (P>0,05) para as características físico-químicas da carne dos cordeiros alimentados com as dietas testadas. Portanto, os resultados indicam que dietas contendo 50% de silagens de genótipos de capim-elefante (IRI-381, Elephant B ou Mott), colhidos aos 60 dias de crescimento, têm potencial para uso na alimentação de cordeiros.

Automotive industry is searching for new ways to improve vehicles’ energy efficiency through mass reduction, using aluminum alloys. This change requires a surface protection to extend the life cycle of the components and aluminum anodization is the most used solution. This research is focused on the intake and exhaust ducts’ surfaces of aluminum internal combustion engines cylinder head, which are subject to chemical agents and temperature variation. To extend the working life of this component it is necessary to obtain a covering protective layer. The process targeted the anodization of an internal surface of a much larger part of a cast aluminum-silicon alloy cylinder head. The anodization was obtained using a H2SO4 solution (184 g.L-1) and a DC voltage starting at 20 V. The Al2O3 layer obtained, inside the cylinder head´s ducts, has an average thickness of 120 µm in accordance with the proposal of providing a suitable surface protection.

Heart rate variability (HRV) is a relevant physiological variable for the estimation of cardiac autonomic function. Although the gold standard for HRV registration is the electrocardiogram (ECG), several applications (APPs) have been increasingly developed. The evaluation carried out by these devices must be compatible with ECG standards. The aim of this study was to compare the data obtained simultaneously with ECG and APP with chest heart rate transmitters. Fifty-six healthy individuals (28 men and 28 women) were evaluated at rest through a short simultaneous HRV measurement with both devices. Data from both acquisition systems were analyzed separately using their own analysis software and exported and analyzed using a validated software. Signal recordings were compatible between the two acquisition systems (Pearson r=0.99; P<0.0001). Although a high correlation was found for the HRV variables obtained in the time domain (Spearman r=0.99; P<0.0001), the correlation decreased in the frequency domain (Pearson r=0.85; P<0.0001) when two software programs were used. Comparison of the averages of spectral analysis parameters also showed differences when HRV data were analyzed separately in each device for low-frequency (LF) and high-frequency (HF) bands. Although the portability of these mobile devices allows for optimal HRV evaluation, the direct analysis obtained from these devices must be carefully evaluated with respect to frequency domain parameters.

Super-austenitic stainless steels (SASS) are alloys with better performance in industrial applications in comparison with standard degrees of austenitic stainless steels (ASS), mainly because of elevated Ni and Mo contents and residual control of certain elements because the improvement of refine techniques. These facts, promotes an improvement of mechanical properties and minimize detrimental effects of intergranular corrosion. Besides, some industrial applications involve high temperatures services, because these alloys families have an excellent creep and oxidation resistances performances. In this work, the objective is evaluate the microstructural and mechanical properties in high Mo SASS exposed at 600˚C in specimens aged until 2000 hours. Thus, hardness and toughness values and Double Loop Electrochemical Potentiodynamic Reactivation (DL-EPR) measurements were analyzed in several thermal aging in comparison to solution treatment condition. Complementary, analysis by Light Optical (LOM), of specimens tested by DL-EPR, and Scanning Electron Microscopies (SEM) in fractured surface from Charpy specimens were also analysed by SEM by backscattered electron mode (BSE). The results show that the aging provokes intergranular embrittlement by inhomogeneous distribution in grain boundaries of Mo rich phase precipitation.

We sought to compare the clinical presentation and prognosis of patients with lung cancer and confirmed COVID-19 infection to those with negative RT-PCR SARS-CoV-2 results. We included patients with confirmed lung cancer and suspected COVID-19 who presented to the emergency department. The primary outcome was in-hospital mortality and secondary outcomes included admission to intensive care unit (ICU) or mechanical ventilation. We analyzed the characteristics according to RT-PCR results and primary outcome. We constructed a logistic regression for each RT-PCR result group to find potential predictors of the primary outcome. Among 110 individuals with confirmed lung cancer (65±9 years, 51% male), 38 patients had positive RT-PCR and 72 patients had negative RT-PCR. There was no difference between groups for any clinical characteristic or comorbidities though individuals with confirmed COVID-19 had higher functionality in the ECOG scale. Leucocytes and lymphocytes were lower in individuals with positive tests. The primary outcome occurred in 58 (53%) individuals, 37 (34%) were admitted to the ICU, and 29 (26%) required mechanical ventilation. Although mortality was similar between the two groups, individuals with confirmed COVID-19 were significantly more likely to be admitted to the ICU or receive mechanical ventilation. Only lower lymphocytes and higher CRP were significantly associated with higher mortality. The clinical presentation of COVID-19 in lung cancer is not sufficient to identify higher or lower probability groups among symptomatic individuals, the overall mortality is high irrespective of RT-PCR results, and lymphopenia on admission was associated with the diagnosis and prognosis for COVID-19.

RESUMO Este estudo avaliou a prevalência e os fatores de risco da ansiedade odontológica entre homens e mulheres. O cálculo amostral foi composto por 244 participantes (n = 122 homens) com 18 anos ou mais que procuraram atendimento odontológico em uma instituição pública de Educação Odontológica no período de março de 2018 a novembro de 2019. A Escala de Ansiedade Odontológica Modificada foi utilizada para determinar a presença de ansiedade odontológica. Os seguintes fatores de risco também foram coletados: idade, anos de estudo, dor pré-operatória e tipo de tratamento odontológico. A análise bivariada foi usada para avaliar a diferença na ansiedade odontológica entre os gêneros. A regressão logística multivariada foi utilizada para analisar a associação entre ansiedade odontológica e gênero, independentemente da influência de outras variáveis. A prevalência total de ansiedade odontológica foi de 18% (n = 44), 22,9% (28/122) nas mulheres e 13,1% (16/122) nos homens (p = 0,04). O gênero (odds ratio: 1,83, intervalo de confiança de 95%: 0,92–3,62) e a dor pré-operatória (odds ratio: 2,095, intervalo de confiança de 95%: 0,97–4,49) foram associados à ansiedade odontológica. Concluímos que as mulheres apresentaram maior prevalência de ansiedade odontológica. A dor pré-operatória foi associada à ansiedade odontológica, independentemente do gênero.

ABSTRACT This study compared prevalence and risk factors of dental anxiety between men and women. The sample consisted of 244 participants (n = 122 men) aged 18 years or older who sought dental care at a public Dental Education Institution from March 2018 to November 2019. The Modified Dental Anxiety Scale was used to determine presence of dental anxiety. The following risk factors were recorded: age, years of schooling, preoperative pain, and type of dental treatment. Bivariate analysis was used to assess the difference in dental anxiety between the sexes. Multivariate logistic regression was used to analyze the association between dental anxiety and gender, regardless of the influence of other variables. Total prevalence of dental anxiety was 18% (n = 44), 22.9% (28/122) in women and 13.1% (16/122) in men (p = 0.04). Gender (odds ratio: 1.83, 95% confidence interval: 0.92–3.62) and preoperative pain (odds ratio: 2.095, 95% confidence interval: 0.97–4.49) were associated with dental anxiety. We concluded that women had a higher prevalence of dental anxiety. Preoperative pain was associated with dental anxiety regardless of gender.

RESUMO A doxorrubicina (dox) é um medicamento antineoplásico que induz cardiotoxicidade por estresse oxidativo. Os flavonoides são antioxidantes extraídos de plantas como Camellia sinensis e Arrabidaea chica (Fridericia chica). Esta pesquisa objetivou avaliar efeitos protetores do extrato de A. chica (AC), comparado ao de C. sinensis (CS), frente ao estresse oxidativo induzido pela dox, no coração. Cardiomiócitos e células neoplásicas MDA-MB 231 foram incubados com AC e CS. Depois, adicionou-se dox e avaliaram-se taxas de viabilidade e morte celular. A citometria de fluxo para o ensaio de iodeto de propídeo (IP) em cardiomiócitos mostrou as seguintes taxas de morte celular: controle 53%; dox 78% (maior que controle, P=0,015); AC_12,5μg/mL + dox 65% (menor que dox, P=0,031); AC_25μg/mL + dox 62% (menor que dox, P=0,028); AC_50μg/mL + dox 63% (menor que dox, P=0,030); CS_12,5μg/mL + dox 71% (menor que dox, P=0,040); CS_25μg/ml + dox 69% (menor que dox, P=0,037); CS_50μg/mL + dox 74% (menor que dox, P=0,044). Resultados das células MDA-MB 231 mostraram que nenhum extrato interferiu na atividade antitumoral da dox. Os dados de IP foram corroborados pelos de MTT. Este estudo reporta promissora utilização de A. chica na prevenção da cardiotoxicidade induzida pela dox.

Physical performance is a multifactorial and complex trait influenced by environmental and hereditary factors. Environmental factors alone have been insufficient to characterize all outstanding phenotypes. Recent advances in genomic technologies have enabled the investigation of whole nuclear and mitochondrial genome sequences, increasing our ability to understand interindividual variability in physical performance. Our objective was to evaluate the association of mitochondrial polymorphic loci with physical performance in Brazilian elite military personnel. Eighty-eight male military personnel who participated in the Command Actions Course of the Army were selected. Total DNA was obtained from blood samples and a complete mitochondrial genome (mtDNA) was sequenced using Illumina MiSeq platform. Twenty-nine subjects completed the training program (FINISHED, ‘F'), and fifty-nine failed to complete (NOT_FINISHED, ‘NF'). The mtDNA from NF was slightly more similar to genomes from African countries frequently related to endurance level. Twenty-two distinct mtDNA haplogroups were identified corroborating the intense genetic admixture of the Brazilian population, but their distribution was similar between the two groups (FST=0.0009). Of 745 polymorphisms detected in the mtDNA, the position G11914A within the NADPH gene component of the electron transport chain, was statistically different between F and NF groups (P=0.011; OR: 4.286; 95%CI: 1.198-16.719), with a higher frequency of the G allele in group F individuals). The high performance of military personnel may be mediated by performance-related genomic traits. Thus, mitochondrial genetic markers such as the ND4 gene may play an important role on physical performance variability.

The aim of this study was to assess the digestibility coefficients (DC) of corn [maize] with an oil content above 3.46% and its effects on the performance of piglets when fed as dry grain (DG) and as rehydrated corn grain silage (RCGS). In Experiment I, 15 piglets (22.51 + 2.39 kg) were allocated to a reference diet (RD) and to two test diets in which corn in the RD was replaced with DG or RCGS. There were five replications of each treatment. Experiment II involved 36 piglets (14.76 ± 2.72 kg), which were assigned to a control diet with common corn grain and to diets in which DG or RCGS replaced the common corn. There were six replications of each treatment. Data were analysed with four statistical models. Model 1 included only the effect of treatment. Model 2 was similar to Model 1 but included initial bodyweight as a covariate. Model 3 was similar to model 1 but included the interaction of diet and period. Model 4 was similar to Model 3 but included the covariate. The more complicated models were generally preferred to Model 1 as they controlled more of the nuisance variation. Feeding a diet that contained RCGS reduced feed intake and improved feed conversion ratio (FCR).

RESUMO O imunofenótipo é considerado um fator prognóstico independente em linfomas de alto grau, visto que os linfomas de origem de células T estão associados a menor tempo de sobrevida. Apesar de vários estudos terem avaliado o perfil imunofenotípico do linfoma nos EUA e na Europa, a pesquisa brasileira sobre o assunto ainda é escassa. A caracterização exata do tipo histopatológico é crucial para estabelecer o tratamento e o prognóstico adequados. Este estudo avaliou a base de dados de laboratórios de imuno-histoquímica que realizam imunofenotipagem do linfoma canino no Brasil. Um total de 203 casos de linfoma multicêntrico foi classificado de acordo com a classificação da OMS. A imunofenotipagem foi capaz de identificar 71,4% dos linfomas da linhagem de células B, 27,1% da linhagem de células T e 1,5% das linhagens de células não B e não T. O linfoma difuso de grandes células B foi o mais comum em 59,1% dos casos. Entre os linfomas de células T, o linfoblástico foi o mais comum (11, 33% dos casos). Embora os linfomas caninos tendam a ser de alto grau, os linfomas indolentes representaram 11,82% dos casos e o linfoma da zona T foi o mais prevalente (8,86%). O imunofenótipo do linfoma multicêntrico no Brasil é semelhante ao de outras partes do mundo, o que sugere fatores etiológicos semelhantes ao desenvolvimento dessa doença.

ABSTRACT The immunophenotype is regarded as an independent prognostic factor in high-grade lymphomas, seeing that lymphomas of T-cell origin are associated with shorter survival time. Although a number of studies have evaluated the immunophenotypical profile of lymphoma in the USA and Europe, Brazilian research on the matter remains scarce. Exact characterization of the histopathological type is crucial to establish proper treatment and prognosis. This study evaluated the database of immunohistochemistry laboratories that perform immunophenotyping of canine lymphoma in Brazil. A total of 203 cases of multicentric lymphoma were classified according to the WHO classification. Immunophenotyping was able to identify 71.4% lymphomas of B-cell line, 27.1% of T-cell line and 1.5% of non-B cells and non-T cell lines. Diffuse large B-cell lymphoma was the most common with 59.1% of the cases. Among T-cell lymphomas, lymphoblastic was the most common (11.33% of the cases). Even though canine lymphomas tend to be high-grade, indolent lymphomas comprised 11.82% of the cases and T-zone lymphoma was the most prevalent (8.86%). The immunophenotype of multicentric lymphoma in Brazil is similar to those in other parts of the world, which suggests similar etiologic factors to the development of this disease.

RESUMO A ultrassonografia convencional e o Doppler espectral nas artérias carótidas comuns em equinos e muares são considerados escassos, diferentemente do que ocorre na medicina humana. Este trabalho propôs comparar as artérias carótidas comuns de equinos e muares por ultrassonografia modo-B e Doppler espectral, além de verificar as diferenças dessas variáveis entre os gêneros desses animais e a relação com a massa corpórea. Foram avaliados os seguintes parâmetros: massa corpórea, diâmetros das artérias, espessura da camada íntima-média (EIM), índice de resistividade (IR), índice de pulsatilidade (IP), velocidade sistólica (VS), velocidade diastólica (VD) e velocidade média (VM). Salvo a massa corpórea, esses parâmetros foram obtidos de três regiões (cranial, médio e caudal) e submetidos à análise conjuntamente. Os diâmetros das artérias carótidas comuns são diferentes entre equinos e muares, com valores menores nos equinos. O IR, o IP, a VS e a VM diferiram entre equinos e muares, sendo maiores nos equinos, e a VD superior nos muares. Não foi observada diferença das variáveis do modo-B e Doppler entre gêneros nos equinos, diferentemente dos muares, cujos machos apresentaram valores maiores do diâmetro, do IR e do IP, mas menores da VS e da VM. A massa corpórea não influenciou as variáveis do modo-B, independentemente do gênero, mas apresenta correlação significativa nas variáveis do modo Doppler. As artérias carótidas comuns de equinos e muares são diferentes pelos exames ultrassonográficos modo-B e Doppler espectral. O gênero não influencia no modo-B e no Doppler nos equinos, porém influencia parcialmente nos muares. A massa corpórea de equinos e de muares, independentemente do gênero, não tem associação com as variáveis do modo-B, apenas com o Doppler.

ABSTRACT Conventional ultrasound and spectral Doppler in the common carotid arteries in horses and mules are considered scarce, different from human medicine. The aim of this study was to compare the common carotid arteries of horses and mules by B-mode ultrasonography and spectral Doppler ultrasonography, as well as to verify the differences of these variables between their genders and the relation with body mass. The following parameters were evaluated: body mass, artery diameter, intima-media thickness (EIM), resistivity index (IR), pulsatility index (IP), systolic velocity (VS), diastolic velocity (VD) and average velocity (VM). Besides the body mass, the other variables were obtained from three regions (cranial, medium and caudal) and analyzed together. The diameters of the common carotid arteries are different between horses and mules, being smaller in horses. The IR, IP, VS and VM differed between horses and mules, being higher in the horses, but the VD was higher in mules. No difference in the variables in B-mode and Doppler between gender were observed in horses, different from mules, in which the diameters, IR and IP values were higher in males and the VS and VM was higher in females. Body mass did not influence B-Mode, both for horses and mules. A significant correlation was observed for Doppler. The common carotid arteries of horses and mules are different by ultrasound scans B-mode and spectral Doppler. Gender does not influence the B-Mode and Doppler variables in horses, but can in mules. The body mass of horses and mules, regardless of gender, is not associated with B-mode variables, but with Doppler variables.

RESUMO: O uso da soja transgênica resistente ao glyphosate pode causar impactos diretos ou indiretos nos componentes do agroecossistema. O impacto direto é provocado pela inserção do gene de resistência, e o indireto está relacionado às alterações nas práticas de manejo. Dessa forma, este trabalho objetivou avaliar o impacto da soja resistente ao glyphosate e seu manejo com esse herbicida sobre a comunidade de artrópodes do dossel das plantas. Os tratamentos foram: soja transgênica e não transgênica com capina mecânica das plantas daninhas; e soja transgênica com uma e três aplicações de glyphosate. As populações de artrópodes no dossel das plantas foram amostradas ao longo de dois cultivos. A incorporação do gene de resistência ao glyphosate não afetou a riqueza e a abundância de artrópodes no dossel das plantas. Já a aplicação do glyphosate reduziu a riqueza de predadores e de fitófagos mastigadores e sugadores nos tratamentos com três aplicações desse herbicida. No segundo ano de cultivo a densidade total de artrópodes foi menor na soja transgênica com três aplicações de glyphosate em relação aos demais tratamentos. O mesmo ocorreu com a densidade do fitófago mastigador Cerotoma arcuatus nos dois anos de cultivo. Já as densidades dos fitófagos succionadores Bemisia tabaci, Caliothrips brasiliensis e Tetranychus sp. foram maiores na soja transgênica com três aplicações de glyphosate do que nos demais tratamentos. A aplicação de glyphosate (uma ou três) reduziu densidades do predador Solenopsis sp. e do detritívoro Hypogastrurasp.

ABSTRACT: This study aims to evaluate the impact of glyphosate-resistant soybean and its management with glyphosate on the canopy arthropod community. We study the direct impact of the insertion of the resistance gene and the indirect impact of management practices, specifically herbicide application. To do this, we use the following treatments: non-GM and GM soybean with mechanical weed control and GM soybean with one and three applications of glyphosate. Arthropods in the crop canopy were sampled over time in all treatments. The incorporation of the glyphosate resistance gene did not affect the richness and abundance of arthropods in the plant canopy. Glyphosate application reduced the richness of predators and chewing and sucking phytophagous arthropods in treatments with three herbicide applications. In the second season, total arthropod density was the lowest in transgenic soybean with three glyphosate applications. The density of Cerotoma arcuatus, a chewing phytophagous arthropod, followed similar trends, while both glyphosate treatments (one or three applications) reduced the densities of the predator Solenopsis sp. and the detritivore Hypogastrura sp. Meanwhile, the densities of the sucking phytophagous arthropods Bemisia tabaci, Caliothrips brasiliensis, and Tetranychus sp. were the highest in transgenic soybean with three glyphosate applications.

RESUMO Estruturas oculares de cães braquicéfalos foram estudadas por elastografia acoustic radiation force impulse (ARFI) para estabelecer valores quantitativos e qualitativos de referência relacionados com a forma e a rigidez de algumas estruturas oculares. Foram avaliados 84 olhos de 42 cães braquicéfalos neste estudo. Os animais foram submetidos aos exames físico, laboratorial e aos exames oftalmológicos. Após a confirmação da sanidade dos pacientes, os cães foram submetidos à ultrassonografia e à elastografia ARFI, enfatizando-se o nervo óptico, o corpo ciliar e a lente. Para elastografia qualitativa, foram observadas características homogêneas no corpo ciliar e no nervo óptico. Os humores aquoso e vítreo foram visibilizados como imagens tipo mosaico. A elastografia quantitativa demonstrou velocidades de cisalhamento para nervo óptico de 1,01±0,27m/s, para corpo ciliar temporal de 0,91±0,24m/s e para corpo ciliar nasal de 0,91±0,3m/s. Valores dessas velocidades para lentes apresentaram-se fora do intervalo: X.XXm/s. A elastografia provou ser um procedimento não invasivo e viável para cães. Estabelecidos os parâmetros do grau de rigidez das estruturas oculares, futuramente tais dados servirão como padrão para avaliação de animais com os distúrbios oculares. Os resultados podem ser extrapolados para a pesquisa primária sobre a aplicabilidade da ARFI na avaliação do bulbo ocular em seres humanos.

ABSTRACT Eyes from brachycephalic dogs were studied by acoustic radiation force impulse (ARFI) elastography to establish quantitative and qualitative reference values related to the shape and rigidity of some ocular structures. Eighty-four eyes from 42 adult brachycephalic dogs were included in this study. Animals were subjected to physical, laboratory, and ophthalmic examinations. Following confirmation of good health, the dogs were subjected to ultrasonography and ARFI elastography, emphasizing the optic nerve, ciliary body, and lens. On qualitative elastography, homogeneous characteristics were observed on the ciliary body and optic nerve. Aqueous and vitreous humors were visualized as mosaic-like images. Quantitative elastography showed the mean optic nerve shear wave velocity (SWV) was 1.01±0.27m/s, temporal ciliary body was 0.91±0.24m/s, and nasal ciliary body was 0.91±0.3m/s. The SWV of the lens values were out of range, this value was not detectable by this software. Elastography has proved to be a non-invasive procedure and feasible in dogs. The establishmentof the parameters of degree of rigidity of ocular structures will serve as a baseline for animals with eye disorders. Results may be extrapolated to primary research on the applicability of ARFI in the evaluation of ocular bulb in humans.

RESUMO: Atualmente, o biomonitoramento tem sido uma metodologia bastante adotada para se verificar o impacto ambiental das novas tecnologias no meio agrícola, com destaque para a entomofauna edáfica, que é tradicionalmente utilizada como bioindicadora nesse tipo de pesquisa. Assim, objetivou-se com este estudo investigar a variação da entomofauna edáfica em função da aplicação do herbicida glyphosate em cultivos de soja Roundup Ready. O experimento foi realizado em Coimbra, MG, nas safras agrícolas de 2007/2008 e 2008/2009. Utilizou-se o delineamento experimental em blocos casualizados com cinco repetições. Os tratamentos estudados foram: soja não transgênica com capina mecânica das plantas daninhas; soja transgênica com capina mecânica das plantas daninhas; soja transgênica com uma aplicação de glyphosate; e soja transgênica com três aplicações de glyphosate. As populações da entomofauna edáfica foram amostradas ao longo dos dois cultivos. A inserção do gene de tolerância ao herbicida glyphosate não afetou a riqueza e a abundância de artrópodes do interior do solo. A riqueza dos artrópodes foi reduzida nos tratamentos que receberam uma e três aplicações de glyphosate. A aplicação de glyphosate na soja transgênica reduziu a densidade do ácaro predador Galumnidae (Acari); das formigas predadoras Neivamyrmex sp. (Hymenoptera: Formicidae) e Solenopsis sp. (Hymenoptera: Formicidae); e dos colêmbolos Entomobryidae (Collembola), Hypogastrura sp. (Collembola: Hypogastruridae) e Onychiuridae (Collembola). Diante disso, torna-se essencial atender às recomendações de uso do herbicida glyphosate e adotar boas práticas culturais que favoreçam a biodegradação de pesticidas, para assim contribuir com a redução do potencial toxicológico do glyphosate sobre a entomofauna edáfica.

ABSTRACT: Currently, biomonitoring is a methodology used to verify the environmental impact of new technologies in the agricultural environment, highlighting edaphic entomofauna that is traditionally used as a bioindicator in this sort of research. Therefore, the objective of this study was to investigate the edaphic entomofauna variation depending on glyphosate application in Roundup Ready soybeans. The experiment was carried out in Coimbra, MG during the 2007/2008 and 2008/2009 cropping seasons. The experimental design was a randomized block design with five replications. The treatments were: non-transgenic soybean with mechanical weeding of weeds; transgenic soybean with mechanical weeding of weeds; transgenic soybean with one glyphosate application and transgenic soybean with three glyphosate applications. The populations of the edaphic entomofauna were sampled during two crops. The insertion of the glyphosate tolerance gene did not affect the richness and the abundance of arthropods in the soil. The arthropod richness was reduced in treatments where glyphosate was applied one and three times. The glyphosate application in transgenic soybean reduced the density of the predatory mite Galumnidae (Acari); predator ants Neivamyrmex sp. (Hymenoptera: Formicidae) and Solenopsis sp. (Hymenoptera: Formicidae); and springtails Entomobryidae (Collembola), Hypogastrura sp. (Collembola: Hypogastruridae) and Onychiuridae (Collembola). Therefore, it is essential to follow the use recommendations of the herbicide glyphosate and adopt good agricultural practices that promote pesticide biodegradation, thereby contributing to the reduction of the toxicological potential of glyphosate on the edaphic entomofauna.