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RESUMEN Objetivo . Desarrollar y validar un método de suspensión celular utilizando células Vero 76 para el cultivo del virus Zika (ZIKV) basado en la infección de células recién sembradas no adheridas. Material y métodos . Se utilizaron tres multiplicidades de infección diferentes del ZIKV para desarrollar y comparar este novedoso método con el método estándar de monocapa de células confluentes. Además, validamos preliminarmente el método de suspensión utilizando muestras clínicas caracterizadas como positivas o negativas para el ZIKV. El método estándar de monocapa se utilizó como método de referencia, y el aislamiento viral se confirmó mediante un RT-PCR específico del ZIKV. Se estimó la sensibilidad e intervalos de confianza del 95% para el método de suspensión. Asimismo, se realizó una comparación técnica del método de suspensión contra el método de monocapa. Resultados . Nuestros hallazgos sugieren que tanto la carga viral como la replicación del ZIKV fueron comparables entre los métodos de infección en monocapa y en suspensión. Aunque ambos métodos fueron adecuados para cultivar y aislar el ZIKV, el método de suspensión se caracterizó por ser más fácil, barato y rápido, así como una técnica de aislamiento sensible. En comparación con el método de monocapa, el método de suspensión fue cuatro veces más sensible en la detección del ZIKV en casos inconclusos por RT-PCR. Conclusiones . El método de suspensión tiene el potencial de ser un método eficaz para cultivar y aislar el ZIKV y su uso es potencialmente útil tanto en la investigación como en entornos clínicos.

ABSTRACT Objective. To develop and validate a cell suspension method using Vero 76 cells for culturing Zika virus (ZIKV) based on infection of detached freshly seeded cells. Material and methods. Three different multiplicities of infection of ZIKV were used to develop and compare this novel method to the standard confluent cell monolayer method. In addition, we preliminary validated the cell suspension method using well-characterized ZIKV positive and negative clinical samples. The standard confluent cell monolayer method was used as the reference method, and viral isolation was confirmed by a ZIKV-specific RT-PCR. The sensitivity and its 95% confidence intervals for the cell suspension method were estimated. Also, a technical comparison of the cell suspension method against the cell monolayer method was performed. Results. Our findings suggested that both the viral load and replication of ZIKV were comparable between both monolayer- and suspension-infection methods. Although both methods were suitable for culturing and isolating ZIKV, the cell suspension method was easier, cheaper, and quicker as well as a sensitive isolation technique. The cell suspension method was significantly more sensitive in detecting Zika in inconclusive cases by RT-PCR, with a fourfold increase compared to the confluent cell monolayer method. Conclusion. The cell suspension method has the potential to be an effective method for cultivating and isolating ZIKV and its application is potentially useful in both research and clinical settings.

https://doi.org/10.17843/rpmesp.2023.403.12606

Molecular characterization of Astyanax species (Characiformes: Characidae) from the upper Paraguaçu River basin, a hydrographic system with high endemism
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Silva-Santos, Rosane

; Machado, Carolina de Barros

; Zanata, Angela Maria

; Camelier, Priscila

; Galetti Jr, Pedro Manoel

; Freitas, Patrícia Domingues de

Neotropical Ichthyology

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2023, Volumen 21 Nº 2 elocation e230032

Resumen: En Pt | Texto: En Pt | PDF: En | PDF: Pt

Abstract Molecular tools have been employed to improve the knowledge about freshwater Neotropical fishes. Such approaches supporting studies of groups including species complexes such as Astyanax, one of the most diversified and taxonomically complex genus of the family Characidae. Here, we employed species delimitation analyses in four Astyanax species described for the upper Paraguaçu River basin, a drainage within Northeastern Mata Atlântica freshwater ecoregion with high endemism. We implemented single and multilocus approaches based on two mitochondrial and one nuclear markers. Cytochrome c Oxidase I sequences previously available for Astyanax species were also added to our dataset. The single locus analyses showed A. epiagos, A. rupestris, and A. aff. rupestris as different Molecular Operational Taxonomic Units (MOTUs), while A. brucutu and A. lorien were grouped. However, the multilocus approach distinguished these two species and showed congruence for the remaining single locus results. Astyanax aff. rupestris was separated into two MOTUs using both approaches, highlighting the need for an integrative taxonomic revision including A. aff. rupestris. These findings contribute to a better understanding of the diversity of this fish group in the upper Paraguaçu, identifying hidden diversity and reinforcing the relevance of this hydrographic system as a notable hotspot for ichthyofauna biodiversity endemism.

Resumo Ferramentas moleculares têm sido empregadas para melhorar o conhecimento sobre os peixes Neotropicais. Tais abordagens apoiam estudos de grupos que incluem complexos de espécies, como Astyanax, um dos gêneros mais diversificados e taxonomicamente complexos dentro da família Characidae. Neste estudo, nós empregamos análises de delimitação de espécies em quatro espécies de Astyanax recentemente descritas da bacia do alto rio Paraguaçu, uma drenagem dentro da ecorregião Mata Atlântica Nordeste que apresenta alto endemismo. Nós realizamos abordagens de loco único e multilocos baseadas em dois marcadores mitocondriais e um nuclear. Sequências de Citocromo c Oxidase I anteriormente disponíveis para espécies de Astyanax foram adicionadas ao nosso conjunto de dados. As análises de loco único mostraram A. epiagos, A. rupestris e A. aff. rupestris como diferentes Unidades Taxonômicas Operacionais Moleculares (MOTUs), enquanto A. brucutu e A. lorien foram agrupadas. Entretanto, a abordagem multilocos distinguiu estas duas espécies e mostrou congruência com os demais resultados das análises de loco único. Astyanax aff. rupestris foi separada em duas MOTUs usando ambas as abordagens, sugerindo a necessidade de uma revisão taxonômica integrativa incluindo A. rupestris e ambas A. aff. rupestris. Esses achados contribuem para uma melhor compreensão da diversidade desse grupo de peixes na bacia do rio Paraguaçu, identificando diversidade oculta e reforçando a relevância desse sistema hidrográfico como um notável hotspot de endemismo da biodiversidade da ictiofauna.

https://doi.org/10.1590/1982-0224-2023-0032

Periodontal diseases and tooth wear in sheep flocks in the State of Goiás, Brazil
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Martins, Andressa S.

; Silva, Tamires A.

; Athayde, Flávia R.F.

; Saraiva, Júlia R.

; Mendes, Juliana G.

; Cardoso, Maria Vitória S.

; Dutra, Iveraldo S.

; Borsanelli, Ana Carolina

Pesquisa Veterinária Brasileira

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2023, Volumen 43 elocation e07367

Resumen: En Pt | Texto: En Pt | PDF: En | PDF: Pt

ABSTRACT: Dental and periodontal affections consist of health concerns of great importance in sheep flocks, as they affect the health and welfare of animals and represent one of the main causes of premature slaughter. Studies on the occurrence of these conditions in sheep are scarce in Brazil. The present study aimed to evaluate the prevalence of periodontal diseases and tooth wear in sheep flocks in the State of Goiás. For this, a clinical oral examination was conducted on 325 sheep from seven farms in six municipalities of the State. Periodontal lesions, characterized by gingival recession in at least one incisor tooth, were observed in 48.3% (157/325) of the sheep, while in cheek teeth it occurred in 13.2% (43/325) of the evaluated sheep. Dental wear on cheek teeth was identified in 69.2% (255/325) of the sheep, and 10.7% (35/325) of the ovines showed dental wear on incisor teeth. Sheep older than 36 months had an increase in the frequency of tooth wear. A total of 171 (52.62%) out of the 325 examined sheep had signs of bleeding on probing, which is compatible with gingivitis, and 69 (40.36%) were older than 36 months. Thirty-eight (11.69%) out of the 325 evaluated ovines had gingivitis in all teeth, among which 21 (55.26%) were older than 36 months. Logistic regression analysis allowed the observation that age was associated with the occurrence of gingivitis. Less frequent changes such as periodontal pockets and loss of at least one tooth were also observed in 4.9% (16/325) and 17.8% (58/325) of the sheep, respectively. It indicates that the presence of periodontal diseases and tooth wear is a common occurrence in the evaluated flocks. Therefore, there is a need to include the oral health of sheep in the routine of the farm as an essential preventive measure to improve the quality of life, animal welfare, and zootechnical indices.

RESUMO: As afecções dentárias e periodontais constituem uma preocupação sanitária de grande importância em rebanhos ovinos, uma vez que afetam a saúde e o bem-estar dos animais, e representam uma das principais causas para o abate prematuro. No Brasil, estudos sobre a ocorrência dessas afecções em ovinos são escassos. Assim, o presente estudo teve como objetivo avaliar a prevalência de doenças periodontais e desgaste dentário em rebanhos ovinos no estado de Goiás. Para isso, realizou-se o exame clínico bucal em 325 ovinos em sete propriedades rurais de seis municípios do estado. As lesões periodontais, caracterizadas por recessão gengival em, pelo menos, um dente incisivo, foram observadas em 48,3% (157/325) dos ovinos, enquanto nos mastigatórios ocorreu em 13,2% (43/325) dos ovinos avaliados. O desgaste dentário em dentes mastigatórios foi identificado em 69,2% (255/325) dos ovinos e 10,7% (35/325) dos ovinos apresentaram desgaste dentário em dentes incisivos. Ovinos com idade superior a 36 meses tiveram um aumento na frequência do desgaste dentário. Dos 325 ovinos examinados, 171 (52,62%) apresentaram sinais de sangramento à sondagem, compatíveis com o quadro de gengivite, sendo que 69 (40,36%) apresentaram idade superior a 36 meses. Dos 325 ovinos avaliados, 38 (11,69%) apresentaram gengivite em todos os dentes, dos quais 21 (55,26%) apresentaram idade superior a 36 meses. Pela análise de regressão logística, foi possível observar que a idade mostrou associação com a ocorrência de gengivite. Alterações menos frequentes como a bolsa periodontal e a perda de, pelo menos, um elemento dentário também foram observadas em 4,9% (16/325) e 17,8% (58/325) dos ovinos, respectivamente. Os resultados do presente estudo indicam que a presença doenças periodontais e desgaste dentário é uma ocorrência comum nos rebanhos avaliados. Portanto, enfatiza-se a necessidade de incluir a saúde bucal dos ovinos na rotina da propriedade como uma medida preventiva essencial para melhorar a qualidade de vida e o bem-estar dos animais, bem como os índices zootécnicos.

https://doi.org/10.1590/1678-5150-pvb-7367

Condiciones de salud de los indígenas hitnü potencialmente expuestos a petróleo crudo (Arauca, Colombia)
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Rivero-Rubio, Carolina

; Navarro-Rodríguez, Angélica I.

; Castro-Reyes, María C.

; Araújo-Quintana, Óscar

; Moreno-Montoya, José

; Estévez-García, Jesús A.

; Martínez-Silva, Pablo A.

; Idrovo, Álvaro J.

; Amaya-Castellanos, Claudia

Biomédica

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Dic 2022, Volumen 42 Nº 4 Paginas 679 - 696

Resumen: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen Introducción. El pueblo hitnü vive en condiciones sanitarias precarias, con inseguridad alimentaria y víctima de la violencia sociopolítica en Arauca (Colombia). Además, se sospecha que pueden estar afectados por la exposición a los hidrocarburos del petróleo. Objetivo. Identificar los eventos de salud del perfil de morbilidad y mortalidad de los indígenas hitnü que podrían asociarse con la exposición a petróleo crudo. Materiales y métodos. Se realizó un estudio transversal con indígenas hitnü, durante febrero y marzo de 2021, época de sequía. Se aplicó un cuestionario de hogares y uno individual para recolectar datos del ambiente peridomiciliario, ocupaciones y otras actividades, así como datos sociodemográficos, signos, síntomas y hallazgos de un examen médico. La potencial asociación con los hidrocarburos se exploró considerando tres grupos, según su localización: cabecera de Arauca, resguardo Aspejená (no expuestos) y resguardos de San José del Lipa y La Vorágine (expuestos por su cercanía al río Ele y afluentes). Con listados libres, se exploraron las causas de muerte. El estudio incorporó un riguroso manejo intercultural en todos sus componentes. Resultados. Participaron 576 indígenas de 16 asentamientos. El agua consumida pudo servir como medio de exposición a los hidrocarburos. Los problemas de salud fueron muy variados e incluían enfermedades infecciosas y crónicas, malnutrición y trauma. Las masas en el cuello se asociaron con residir en los resguardos ancestrales (RP=3,86; IC95% 1,77-8,39), territorios potencialmente expuestos al petróleo. Las causas de muerte más relevantes fueron el homicidio, los tumores y la tuberculosis. Conclusión: Por su posible asociación con los hidrocarburos, es prioritario el estudio intercultural de linfoadenopatías entre indígenas potencialmente expuestos al petróleo.

Abstract Introduction: The Hitnü indigenous people live in precarious sanitary conditions, with food insecurity and being victims of sociopolitical violence in Arauca, Colombia. In addition, it is possible that they may be affected by exposure to hydrocarbons found in oil. Objective: To identify the health outcomes of morbidity and mortality profiles of the Hitnü people that could be associated with the exposure to crude oil. Materials and methods: A cross-sectional study was carried out with Hitnü indigenous people, during February and March, 2021, time of drought. A household questionnaire was applied, and one individual to collect data from the environment around the house, occupations and other activities, as well as data from sociodemographic, signs, symptoms, and findings of a medical examination. The potential association with hydrocarbons was explored considering three groups: inhabitants in Arauca city, Aspejaná reserve (not exposed), and San José del Lipa and La Vorágine reserves (exposed by the Ele river and tributaries). With free listings, causes of death were explored. The study incorporated a rigorous intercultural management in all its components. Results: A total of 576 indigenous people from 16 settlements participated. The water consumed could serve as means of exposure to hydrocarbons. Health problems were very varied, including infectious and chronic diseases, malnutrition, and trauma. The masses on the neck were associated with residing in the ancestral reserves (PR = 3.86; CI95% 1.77-8.39), territories with potential exposure to crude-oil. The most relevant causes of death were homicide, tumors, and tuberculosis. Conclusion: For its possible association with exposure to hydrocarbons, it is a priority to start the intercultural study of lymphadenopathies in indigenous communities potentially exposed to crude oil.

https://doi.org/10.7705/biomedica.6591

[SciELO Preprints] - Guideline of the Brazilian Society of Cardiology on Diagnosis and Treatment of Patients with Chagas Disease Cardiomyopathy
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Marin-Neto, José Antonio Rassi Jr., Anis Moraes Oliveira, Gláucia M. Lemos Correia, Luís Claudio Novaes Ramos Jr., Alberto Hasslocher-Moreno, Alejandro Marcel Luquetti Ostermayer, Alejandro Sousa, Andréa Silvestre de Amato Vincenzo de Paola, Angelo Sobral de Sousa, Antonio Carlos Pinho Ribeiro, Antonio Luiz Correia Filho, Dalmo Moraes de Souza, Dilma do Socorro Cunha-Neto, Edecio J. A. Ramires, Felix Bacal, Fernando Pereira Nunes, Maria do Carmo Martinelli Filho, Martino Ibrahim Scanavacca, Maurício Magalhães Saraiva, Roberto Alves de Oliveira Júnior, Wilson M. Lorga-Filho, Adalberto de Jesus Benevides de Almeida Guimarães, Adriana Lopes Latado Braga, Adriana Sarmento de Oliveira, Adriana V. L. Sarabanda, Alvaro Yecê das Neves Pinto, Ana Assis Lopes do Carmo, André Schmidt, André Costa, Andréa Rodrigues da Ianni, Barbara Maria Markman Filho, Brivaldo Eduardo Rochitte, Carlos Thé Macedo, Carolina Mady, Charles Chevillard, Christophe Bittencourt das Virgens, Cláudio Marcelo Nery de Castro, Cleudson De Paoli de Carvalho Britto, Constança Felícia Pisani, Cristiano do Carmo Rassi, Daniela C. Sobral Filho, Dario Rodrigues Almeida, Dirceu A. Bocchi, Edimar T. Mesquita, Evandro de Souza Nogueira Sardinha Mendes, Fernanda Pereira, Francisca Tatiana Sperandio da Silva, Gilberto Marcelo de Lima Peixoto, Giselle Glotz de Lima, Gustavo H. Veloso, Henrique Turin Moreira, Henrique Bellotti Lopes, Hugo Masciarelli Francisco Pinto, Ibraim Pinto Dias, João Carlos Bemfica, João Marcos Silva-Nunes, João Paulo Soares Barreto-Filho, José Augusto Kerr Saraiva, José Francisco Lannes-Vieira, Joseli Menezes Oliveira, Joselina Luzia V. Armaganijan, Luciana Martins, Luiz Cláudio C. Sangenis, Luiz Henrique Barbosa, Marco Paulo Almeida-Santos, Marcos Antônio Simões, Marcos Vinicius Shikanai-Yasuda, Maria Aparecida Vieira Moreira, Maria da Consolação Higuchi, Maria de Lourdes Costa Monteiro, Maria Rita de Cássia Felix Mediano, Mauro Felippe Maia Lima, Mayara T. Oliveira, Maykon Moreira Dias Romano , Minna Nitz, Nadjar de Tarso Jorge Medeiros, Paulo Vieira Alves, Renato Alkmim Teixeira, Ricardo Coury Pedrosa, Roberto Aras, Roque Morais Torres, Rosália dos Santos Povoa, Rui Manoel Rassi, Sérgio Gabriel Salles Xavier, Sérgio Marinho Martins Alves , Silvia B. N. Tavares, Suelene Lima Palmeira, Swamy da Silva Junior, Telêmaco Luiz da Rocha Rodrigues, Thiago Madrini Junior, Vagner Maia da Costa , Veruska Dutra, Walderez

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Fecha de publicación: 2022-10-11

Resumen: En Pt

This guideline aimed to update the concepts and formulate the standards of conduct and scientific evidence that support them, regarding the diagnosis and treatment of the Cardiomyopathy of Chagas disease, with special emphasis on the rationality base that supported it.nbsp; Chagas disease in the 21st century maintains an epidemiological pattern of endemicity in 21 Latin American countries. Researchers and managers from endemic and non-endemic countries point to the need to adopt comprehensive public health policies to effectively control the interhuman transmission of T. cruzi infection, and to obtain an optimized level of care for already infected individuals, focusing on diagnostic and therapeutic opportunistic opportunities. nbsp; Pathogenic and pathophysiological mechanisms of the Cardiomyopathy of Chagas disease were revisited after in-depth updating and the notion that necrosis and fibrosis are stimulated by tissue parasitic persistence and adverse immune reaction, as fundamental mechanisms, assisted by autonomic and microvascular disorders, was well established. Some of them have recently formed potential targets of therapies.nbsp; The natural history of the acute and chronic phases was reviewed, with enhancement for oral transmission, indeterminate form and chronic syndromes. Recent meta-analyses of observational studies have estimated the risk of evolution from acute and indeterminate forms and mortality after chronic cardiomyopathy. Therapeutic approaches applicable to individuals with Indeterminate form of Chagas disease were specifically addressed. All methods to detect structural and/or functional alterations with various cardiac imaging techniques were also reviewed, with recommendations for use in various clinical scenarios. Mortality risk stratification based on the Rassi score, with recent studies of its application, was complemented by methods that detect myocardial fibrosis.nbsp; The current methodology for etiological diagnosis and the consequent implications of trypanonomic treatment deserved a comprehensive and in-depth approach. Also the treatment of patients at risk or with heart failure, arrhythmias and thromboembolic events, based on pharmacological and complementary resources, received special attention. Additional chapters supported the conducts applicable to several special contexts, including t. cruzi/HIV co-infection, risk during surgeries, in pregnant women, in the reactivation of infection after heart transplantation, and others.nbsp; nbsp;nbsp; Finally, two chapters of great social significance, addressing the structuring of specialized services to care for individuals with the Cardiomyopathy of Chagas disease, and reviewing the concepts of severe heart disease and its medical-labor implications completed this guideline.

Esta diretriz teve como objetivo principal atualizar os conceitos e formular as normas de conduta e evidências científicas que as suportam, quanto ao diagnóstico e tratamento da CDC, com especial ênfase na base de racionalidade que a embasou. A DC no século XXI mantém padrão epidemiológico de endemicidade em 21 países da América Latina. Investigadores e gestores de países endêmicos e não endêmicos indigitam a necessidade de se adotarem políticas abrangentes, de saúde pública, para controle eficaz da transmissão inter-humanos da infecção pelo T. cruzi, e obter-se nível otimizado de atendimento aos indivíduos já infectados, com foco em oportunização diagnóstica e terapêutica. Mecanismos patogênicos e fisiopatológicos da CDC foram revisitados após atualização aprofundada e ficou bem consolidada a noção de que necrose e fibrose sejam estimuladas pela persistência parasitária tissular e reação imune adversa, como mecanismos fundamentais, coadjuvados por distúrbios autonômicos e microvasculares. Alguns deles recentemente constituíram alvos potenciais de terapêuticas. A história natural das fases aguda e crônica foi revista, com realce para a transmissão oral, a forma indeterminada e as síndromes crônicas. Metanálises recentes de estudos observacionais estimaram o risco de evolução a partir das formas aguda e indeterminada e de mortalidade após instalação da cardiomiopatia crônica. Condutas terapêuticas aplicáveis aos indivíduos com a FIDC foram abordadas especificamente. Todos os métodos para detectar alterações estruturais e/ou funcionais com variadas técnicas de imageamento cardíaco também foram revisados, com recomendações de uso nos vários cenários clínicos. Estratificação de risco de mortalidade fundamentada no escore de Rassi, com estudos recentes de sua aplicação, foi complementada por métodos que detectam fibrose miocárdica. A metodologia atual para diagnóstico etiológico e as consequentes implicações do tratamento tripanossomicida mereceram enfoque abrangente e aprofundado. Também o tratamento de pacientes em risco ou com insuficiência cardíaca, arritmias e eventos tromboembólicos, baseado em recursos farmacológicos e complementares, recebeu especial atenção. Capítulos suplementares subsidiaram as condutas aplicáveis a diversos contextos especiais, entre eles o da co-infecção por T. cruzi/HIV, risco durante cirurgias, em grávidas, na reativação da infecção após transplante cardíacos, e outros.nbsp;nbsp;nbsp; Por fim, dois capítulos de grande significado social, abordando a estruturação de serviços especializados para atendimento aos indivíduos com a CDC, e revisando os conceitos de cardiopatia grave e suas implicações médico-trabalhistas completaram esta diretriz.nbsp;

https://doi.org/10.1590/SciELOPreprints.4820

Chronic inflammatory diseases, subclinical atherosclerosis, and cardiovascular diseases: Design, objectives, and baseline characteristics of a prospective case-cohort study ‒ ELSA-Brasil
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Bensenor, Isabela M.

; Goulart, Alessandra C.

; Pereira, Alexandre C.

; Brunoni, André R.

; Alencar, Airlane

; Santos, Raul D.

; Bittencourt, Márcio S.

; Telles, Rosa W.

; Machado, Luciana Andrade Carneiro

; Barreto, Sandhi Maria

; de Almeida-Pititto, Bianca

; Janovsky, Carolina Porto Silva

; Sgarbi, José Augusto

; Tebar, William R.

; Meneghini, Vandrize

; Barbosa Junior, Fernando

; Ribeiro, Ana Cristina de Medeiros

; Pasoto, Sandra Gofinet

; Pereira, Rosa Maria R.

; Bonfá, Eloísa

; Sipahi, Aytan M.

; Santos, Itamar de S.

; Lotufo, Paulo A.

Clinics

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2022, Volumen 77 elocation 100013

Resumen: En | Texto: En | PDF: En

Abstract Objectives This analysis describes the protocol of a study with a case-cohort to design to prospectively evaluate the incidence of subclinical atherosclerosis and Cardiovascular Disease (CVD) in Chronic Inflammatory Disease (CID) participants compared to non-diseased ones. Methods A high-risk group for CID was defined based on data collected in all visits on self-reported medical diagnosis, use of medicines, and levels of high-sensitivity C-Reactive Protein >10 mg/L. The comparison group is the Aleatory Cohort Sample (ACS): a group with 10% of participants selected at baseline who represent the entire cohort. In both groups, specific biomarkers for DIC, markers of subclinical atherosclerosis, and CVD morbimortality will be tested using weighted Cox. Results The high-risk group (n = 2,949; aged 53.6 ± 9.2; 65.5% women) and the ACS (n=1543; 52.2±8.8; 54.1% women) were identified. Beyond being older and mostly women, participants in the high-risk group present low average income (29.1% vs. 24.8%, p < 0.0001), higher BMI (Kg/m2) (28.1 vs. 26.9, p < 0.0001), higher waist circumference (cm) (93.3 vs. 91, p < 0.0001), higher frequencies of hypertension (40.2% vs. 34.5%, p < 0.0001), diabetes (20.7% vs. 17%, p = 0.003) depression (5.8% vs. 3.9%, p = 0.007) and higher levels of GlycA a new inflammatory marker (p < 0.0001) compared to the ACS. Conclusions The high-risk group selected mostly women, older, lower-income/education, higher BMI, waist circumference, and of hypertension, diabetes, depression, and higher levels of GlycA when compared to the ACS. The strategy chosen to define the high-risk group seems adequate given that multiple sociodemographic and clinical characteristics are compatible with CID.

https://doi.org/10.1016/j.clinsp.2022.100013

High lethality rate of carbapenem-resistant Acinetobacter baumannii in Intensive Care Units of a Brazilian hospital: An epidemiologic surveillance study
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Kurihara, Mariana Neri Lucas

; Sales, Romário Oliveira de

; Silva, Késia Esther da

; Silva, Gerlaine Damasceno

; Mansano, Maria Carolina Tazinazzo

; Mahmoud, Fuad Fayez

; Simionatto, Simone

Revista da Sociedade Brasileira de Medicina Tropical

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2022, Volumen 55 elocation e0529-2021

Resumen: En | Texto: En | PDF: En

ABSTRACT Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) is a growing threat to public health. Methods: A 3-year retrospective study was conducted to evaluate the prevalence and lethality of multidrug-resistant (MDR) A. baumannii isolated from Brazilian patients. Results: In this study, 219 Acinetobacter baumannii isolates were identified, of which 70.8% (155/219) were isolated from patients hospitalized in intensive care units. Of these, 57.4% (n = 89/155) were assessed, of which 92.1% (82/89) were carbapenem-resistant, and 49 were classified as infected. The lethality rate was 79.6% (39/49). Conclusions: We highlight the need of an effective epidemiological surveillance measure to contain the dissemination of CRAB in the hospital environment.

https://doi.org/10.1590/0037-8682-0529-2021

Consenso de leucemia mieloide aguda en México
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Arana-Luna, Luara L.

; Alvarado-Ibarra, Martha

; Silva-Michel, Luis G.

; Morales-Maravilla, Adrián

; González-Rubio, María del C.

; Chávez-Aguilar, Lénica A.

; Tena-Iturralde, María Fernanda

; Mojica-Balceras, Liliana

; Zapata-Canto, Nidia

; Galindo-Delgado, Patricia

; Miranda-Madrazo, María Raquel

; Morales-Hernández, Alba E.

; Silva-Vera, Karina

; Grimaldo-Gómez, Flavio A.

; Hernández-Caballero, Álvaro

; Bates-Martin, Ramón A.

; Álvarez-Vera, José L.

; Tepepa-Flores, Fredy

; Teomitzi-Sánchez, Óscar

; Fermín-Caminero, Denisse J.

; Peña-Celaya, José A. de la

; Salazar-Ramírez, Óscar

; Flores-Villegas, Luz V.

; Guerra-Alarcón, Lidia V.

; Leyto-Cruz, Faustino

; Inclán-Alarcón, Sergio I.

; Milán-Salvatierra, Andrea I.

; Ventura-Enríquez, Yanet

; Pérez-Lozano, Uendy

; Báez-Islas, Pamela E.

; Tapia-Enríquez, Ana L.

; Palma-Moreno, Orlando G.

; Aguilar-Luévano, Jocelyn

; Espinosa-Partida, Arturo

; Pérez-Jacobo, Luis F.

; Rojas-Castillejos, Flavio

; Ruiz-Contreras, Josué I.

; Loera-Fragoso, Sergio J.

; Medina-Coral, Jesús E.

; Acosta-Maldonado, Brenda L.

; Soriano-Mercedes, Emely J.

; Saucedo-Montes, Erick E.

; Valero-Saldana, Luis M.

; González-Prieto, Susana G.

; Nava-Villegas, Lorena

; Hernández-Colin, Ana K.

; Hernández-Alcántara, Areli E.

; Zárate-Rodríguez, Pedro A.

; Ignacio-Ibarra, Gregorio

; Meillón-García, Luis A.

; Espinosa-Bautista, Karla A.

; Ledesma de la Cruz, Cindy

; Barbosa-Loría, Diego M.

; García-Castillo, Carolina

; Balderas-Delgado, Carolina

; Cabrera-García, Álvaro

; Pérez-Zúñiga, Juan M.

; Hernández-Ruiz, Eleazar

; Villela-Peña, Atenas

; Gómez Cortés, Sue Cynthia

; Romero-Rodelo, Hilda

; Garzón-Velásquez, Katheryn B.

; Serrano-Hernández, Cristina

; Martínez-Ríos, Annel

; Pedraza-Solís, María Luisa

; Martínez-Coronel, Jorge A.

; Narváez-Davalos, Iris M.

; García-Camacho, Alinka S.

; Merino-Pasaye, Laura E.

; Aguilar-Andrade, Carolina

; Aguirre-Domínguez, Juan A.

; Guzmán-Mera, Pedro G.

; Delgado-de la Rosa, Elizabeth

; Flores López, Perla E.

; González-Aguirre, Lilia L.

; Ramírez-Alfaro, Edgar M.

; Vera-Calderón, Heidi

; Meza-Dávalos, María Lizeth

; Murillo-Cruz, Juan

; Pichardo-Cepín, Yayra M.

; Ramírez-Romero, Eva F.

Gaceta médica de México

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2022, Volumen 158 Nº spe3 Paginas M1 - M51

Resumen: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Acute myeloid leukemia (AML) comprises a heterogeneous group of hematopoietic cell neoplasms of myeloid lineage that arise from the clonal expansion of their precursors in the bone marrow, interfering with cell differentiation, leading to a syndrome of bone marrow failure. AML is a consequence of genetic and epigenetic changes (point mutations, gene rearrangements, deletions, amplifications, and arrangements in epigenetic changes that influence gene expression) in hematopoietic precursor cells, which create a clone of abnormal cells that are capable of proliferating but cannot differentiate into mature hematopoietic cells or undergo programmed cell death. The diagnosis requires more than 20% myeloid blasts in the bone marrow and certain cytogenic abnormalities. Treatment will depend on age, comorbidities, and cytogenetic risk among the most frequent.

resumen está disponible en el texto completo

https://doi.org/10.24875/gmm.m21000597 895 descargas

Development and validation of predictive model for long-term hospitalization, readmission, and in-hospital death of patients over 60 years old
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Costa, Maria Luiza Monteiro

; Mafra, Ana Carolina Cintra Nunes

; Cendoroglo, Maysa Seabra

; Rodrigues, Patrícia Silveira

; Ferreira, Milene Silva

; Studenski, Stephanie A.

; Franco, Fábio Gazelato de Mello

einstein (São Paulo)

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2022, Volumen 20 elocation eAO8012

Resumen: En | Texto: En | PDF: En

ABSTRACT Objective To develop and validate a high-risk predictive model that identifies, at least, one common adverse event in older population: early readmission (up to 30 days after discharge), long hospital stays (10 days or more) or in-hospital deaths. Methods This was a retrospective cohort study including patients aged 60 years or older (n=340) admitted at a 630-beds tertiary hospital, located in the city of São Paulo, Brazil. A predictive model of high-risk indication was developed by analyzing logistical regression models. This model prognostic capacity was assessed by measuring accuracy, sensitivity, specificity, and positive and negative predictive values. Areas under the receiver operating characteristic curve with 95% confidence intervals were also obtained to assess the discriminatory power of the model. Internal validation of the prognostic model was performed in a separate sample (n=168). Results Statistically significant predictors were identified, such as current Barthel Index, number of medications in use, presence of diabetes mellitus, difficulty chewing or swallowing, extensive surgery, and dementia. The study observed discrimination model acceptance in the construction sample 0.77 (95% confidence interval: 0.71-0.83) and good calibration. The characteristics of the validation samples were similar, and the receiver operating characteristic curve area was 0.687 (95% confidence interval: 0.598-0.776). We could assess an older patient’s adverse health events during hospitalization after admission. Conclusion A predictive model with acceptable discrimination was obtained, with satisfactory results for early readmission (30 days), long hospital stays (10 days), or in-hospital death.

https://doi.org/10.31744/einstein_journal/2022ao8012

10.

Shaping ability of reciprocating and rotary systems in oval-shaped root canals: a microcomputed tomography study
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Medeiros, Thamires C de

; Lima, Carolina O de

; Barbosa, Ana Flávia A

; Augusto, Carla M

; Bruno, Adília Maria V

; Lopes, Ricardo T

; Amoroso-Silva, Pablo A.

; Marceliano-Alves, Marília FV

Acta Odontológica Latinoamericana

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Dic 2021, Volumen 34 Nº 3 Paginas 282 - 288

Resumen: PT En | Texto: PT En | PDF: PT | PDF: En

RESUMO Este estudo comparou a capacidade de modelagem dos sistemas reciprocante de lima única (WaveOne Gold) e rotatórios com múltiplas limas (Mtwo) em caninos ovais inferiores, usando microtomografia computadorizada (micro-CT). Trinta caninos inferiores foram escaneados por micro-CT e divididos em dois grupos (n = 15) de acordo com o sistema usado durante o preparo do canal radicular: WaveOne Gold (WOG) e Mtwo. Os dentes foram reeescaneados e a porcentagem de área do canal não preparada, transporte apical e capacidade de centralização foram analisados. Os dados foram avaliados pelos testes de Kruskal Wallis e Mann-Whitney (p<0,05). Nenhuma diferença foi encontrada na porcentagem de área não preparada entre os grupos em todo o canal radicular e no terço apical e na capacidade de centralização (p>0,05). O sistema WOG promoveu menor transporte do canal do que o sistema Mtwo na região de 5mm aquém do ápice (p<0,05). Os sistemas WOG e Mtwo apresentaram capacidade de modelagem e capacidade de centralização semelhantes em canais ovais. No entanto, WOG promoveu menor transporte do que Mtwo a 5 mm do ápice.

ABSTRACT This study compared the shaping ability of single-file reciprocating (WaveOne Gold) and multifile rotary (Mtwo) systems on mandibular oval-shaped canine root canals, using microcomputed tomography (micro-CT). Thirty mandibular canines were scanned by micro-CT and assigned to one of two groups (n=15) according to the system used for root canal preparation: WaveOne Gold or Mtwo. After preparation, the teeth were rescanned, and the percentage of untouched canal area, apical transportation and centering ability were analyzed. The data was evaluated using Kruskal and Mann- Whitney tests (p<0.05). No difference was found in percentage of unprepared canal area between groups in the entire root canal or the apical third, or in centering ability (p>0.05). WaveOne gold had less canal transportation than MTwo at the 5 mm section (p<0.05). WOG and Mtwo systems presented similar shaping ability and centering ability in oval-shaped canals. However, WOG presented less transportation than Mtwo at 5 mm from the apex.

https://doi.org/10.54589/aol.34/3/282 36 descargas

11.

Incidence of TPOAb over a 4-year follow-up period: results from the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)
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Benseñor, Isabela M.

; Janovsky, Carolina Castro Porto Silva

; Goulart, Alessandra C.

; Santos, Itamar de Souza

; Diniz, Maria de Fátima Haueisen Sander

; Almeida-Pititto, Bianca de

; Sgarbi, José Augusto

; Lotufo, Paulo A.

Archives of Endocrinology and Metabolism

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Dic 2021, Volumen 65 Nº 6 Paginas 832 - 840

Resumen: En | Texto: En | PDF: En

ABSTRACT Objective: Although some previous data have suggested a high iodine intake in Brazil, the prevalence of antithyroperoxidase antibodies (TPOAb) in the country is compatible with rates from countries with adequate iodine intake. This observation emphasizes the importance of knowing the incidence of TPOAb in Brazil. Materials and methods: This prospective analysis included euthyroid participants with negative TPOAb at baseline and a thyroid function assessment at a 4-year follow-up. TPOAb was measured by electrochemiluminescence and considered positive when titers were ≥34 IU/mL. TSH and free T4 (FT4) levels were determined by a third-generation immunoenzymatic assay. The incidence of TPOAb is expressed in percentage per year or as a cumulative incidence within the 4-year follow-up period. Results: Of 8,922 euthyroid participants (mean age 51.1 years; 50.9% women) with a negative TPOAb test at baseline, 130 presented incident TPOAb at the 4-year follow-up, yielding an annual incidence of TPOAb of 0.38%/year (95% confidence interval [95% CI], 0.37-0.39%/year) and a cumulative incidence over 4 years of 1.46% (95% CI, 1.21-1.71%). In men, the annual incidence was 0.32% (95% CI, 0.31-0.33%), and the cumulative incidence over 4 years was 1.23% (95% CI, 0.90-1.56%). In women, the annual incidence was 0.43%/year (95% CI, 0.42-0.44%/year) and the cumulative incidence over 4 years was 1.67% (95% CI, 1.30-2.04%). The only factor associated with incident TPOAb was the occurrence of thyroid diseases at follow-up. No differences in TPOAb incidence were detected across ELSA-Brasil research centers. Conclusion: Based on the results of this study, the incidence of TPOAb per year and at a 4-year follow-up period are compatible with those of a country with adequate iodine intake.

https://doi.org/10.20945/2359-3997000000422 2 descargas

12.

Incidence of thyroid diseases: Results from the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil)
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Benseñor, Isabela M.

; Sgarbi, José Augusto

; Janovsky, Carolina Castro Porto Silva

; Pittito, Bianca Almeida

; Diniz, Maria de Fátima Haueisen Sander

; Almeida, Maria da Conceição Chagas de

; Alvim, Sheila Maria

; Barreto, Sandhi M.

; Giatti, Luana

; Duncan, Bruce B.

; Schmidt, Maria Inês

; Fonseca, Maria de Jesus M.

; Griep, Rosane H.

; Molina, Maria del Carmen B.

; Mill, José Geraldo

; Santos, Itamar de Souza

; Goulart, Alessandra C.

; Lotufo, Paulo A.

Archives of Endocrinology and Metabolism

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Ago 2021, Volumen 65 Nº 4 Paginas 468 - 478

Resumen: En | Texto: En | PDF: En

ABSTRACT Objective: To evaluate incidence of subclinical and overt hyperthyroidism and hypothyroidism. Subjects and methods: The Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) is a prospective cohort study of 15,105 civil servants, examined at baseline and over a 4-year follow-up. This analysis included 9,705 participants with normal thyroid function at baseline, follow-up information about thyroid function and with no report of using drugs that may interfere in the thyroid function. Thyroid function was defined by TSH/FT4 levels or routine use of thyroid hormones/anti-thyroid medications. Annual and cumulative (over 4-year) incidence rates were presented as percentages (95% Confidence Intervals). Results: The incidence of all overt and subclinical thyroid disease was 6.7% (1.73%/year): 0.19% for overt hyperthyroidism (0.048%/year), 0.54% for subclinical hyperthyroidism (0.14%/year), 1.98% for overt hypothyroidism (0.51%/year), and 3.99% for subclinical hypothyroidism (1.03%/year). The incidence of all thyroid diseases was higher in women, when compared to men, with a low women:men ratio (1.36). For Blacks the highest incidence was for overt hyperthyroidism, while for Whites, the highest incidence was for overt hypothyroidism. However, the highest incidence of overt hyperthyroidism was detected in Asian descendants. The presence of antithyroperoxidase antibodies at baseline was associated with higher incidence of overt thyroid diseases. Conclusion: These results showed a high incidence of hypothyroidism, which is compatible with a country with a more-than-adequate iodine intake. The low women:men ratio of the incidence of thyroid dysfunction highlights the importance of the diagnosis of thyroid diseases among men in Brazil.

https://doi.org/10.20945/2359-3997000000348 273 descargas

13.

A Systematic Pipeline to Enhance the Fecal Metabolome Coverage by LC-HRMS
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Alves, Marina A.

; Silva, Ana Carolina R. da

; Torres, Clarisse L.

; Almeida, Lana R. de

; Mastella, Ana Maria O.

; Borges, Ricardo M.

; Garrett, Rafael

Journal of the Brazilian Chemical Society

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Jul 2021, Volumen 32 Nº 7 Paginas 1435 - 1446

Resumen: En | Texto: En | PDF: En

The comprehended knowledge of the metabolic profile of the fecal matter has been recognized as an important point for understanding metabolic changes in the human systemic metabolism and it can provide precious information about host-gut microbiota interactions. However, few analytical strategies have been addressed for a broad analysis of metabolites with different chemical properties to better understand the chemical space of fecal samples. Here we report a systematic pipeline to achieve comprehensive coverage of the fecal metabolome, from high polar to nonpolar metabolites, using dog fecal samples as a proof-of-concept. This pipeline comprises a monophasic (ACN/H2O) and a biphasic extraction (methyl tert-butyl ether (MTBE)/MeOH/H2O) of the sample, followed by three liquid chromatography-high resolution tandem mass spectrometry (LC-HRMS/MS) methods using HILIC-amide, RP-C18 and CSH-C18 columns, and a switch polarity acquisition mode in the electrospray ion source. This approach allowed the annotation of 376 metabolites from 70 different chemical classes. The chemical space analysis by molecular networking and the pathway analysis revealed the complexity of the fecal sample and the importance of combined methods to better understand biochemical pathways. This pipeline can be used as a valuable tool to comprehend the relationship between host-gut microbiota metabolites and the influence of diet, medication, or environmental changes.

https://doi.org/10.21577/0103-5053.20210042 108 descargas

14.

The Program for Biodiversity Research in Brazil: The role of regional networks for biodiversity knowledge, dissemination, and conservation
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ROSA, CLARISSA

; BACCARO, FABRICIO

; CRONEMBERGER, CECILIA

; HIPÓLITO, JULIANA

; BARROS, CLAUDIA FRANCA

; RODRIGUES, DOMINGOS DE JESUS

; NECKEL-OLIVEIRA, SELVINO

; OVERBECK, GERHARD E.

; DRECHSLER-SANTOS, ELISANDRO RICARDO

; ANJOS, MARCELO RODRIGUES DOS

; FERREGUETTI, ÁTILLA C.

; AKAMA, ALBERTO

; MARTINS, MARLÚCIA BONIFÁCIO

; TOMAS, WALFRIDO MORAES

; SANTOS, SANDRA APARECIDA

; FERREIRA, VANDA LÚCIA

; CUNHA, CATIA NUNES DA

; PENHA, JERRY

; PINHO, JOÃO BATISTA DE

; SALIS, SUZANA MARIA

; DORIA, CAROLINA RODRIGUES DA COSTA

; PILLAR, VALÉRIO D.

; PODGAISKI, LUCIANA R.

; MENIN, MARCELO

; BÍGIO, NARCÍSIO COSTA

; ARAGÓN, SUSAN

; MANZATTO, ANGELO GILBERTO

; VÉLEZ-MARTIN, EDUARDO

; SILVA, ANA CAROLINA BORGES LINS E

; IZZO, THIAGO JUNQUEIRA

; MORTATI, AMANDA FREDERICO

; GIACOMIN, LEANDRO LACERDA

; ALMEIDA, THAÍS ELIAS

; ANDRÉ, THIAGO

; SILVEIRA, MARIA AUREA PINHEIRO DE ALMEIDA

; SILVEIRA, ANTÔNIO LAFFAYETE PIRES DA

; MESSIAS, MARILUCE REZENDE

; MARQUES, MARCIA C.M.

; PADIAL, ANDRE ANDRIAN

; MARQUES, RENATO

; BITAR, YOUSZEF O.C.

; SILVEIRA, MARCOS

; MORATO, ELDER FERREIRA

; PAGOTTO, RUBIANI DE CÁSSIA

; STRUSSMANN, CHRISTINE

; MACHADO, RICARDO BOMFIM

; AGUIAR, LUDMILLA MOURA DE SOUZA

; FERNANDES, GERALDO WILSON

; OKI, YUMI

; NOVAIS, SAMUEL

; FERREIRA, GUILHERME BRAGA

; BARBOSA, FLÁVIA RODRIGUES

; OCHOA, ANA C.

; MANGIONE, ANTONIO M.

; GATICA, AILIN

; CARRIZO, MARÍA CELINA

; RETTA, LUCÍA MARTINEZ

; JOFRÉ, LAURA E.

; CASTILLO, LUCIANA L.

; NEME, ANDREA M.

; RUEDA, CARLA

; TOLEDO, JOSÉ JULIO DE

; GRELLE, CARLOS EDUARDO VIVEIROS

; VALE, MARIANA M.

; VIEIRA, MARCUS VINICIUS

; CERQUEIRA, RUI

; HIGASHIKAWA, EMÍLIO MANABU

; MENDONÇA, FERNANDO PEREIRA DE

; GUERREIRO, QUÊZIA LEANDRO DE MOURA

; BANHOS, AUREO

; HERO, JEAN-MARC

; KOBLITZ, RODRIGO

; COLLEVATTI, ROSANE GARCIA

; SILVEIRA, LUÍS FÁBIO

; VASCONCELOS, HERALDO L.

; VIEIRA, CECÍLIA RODRIGUES

; COLLI, GUARINO RINALDI

; CECHIN, SONIA ZANINI

; SANTOS, TIAGO GOMES DOS

; FONTANA, CARLA S.

; JARENKOW, JOÃO A.

; MALABARBA, LUIZ R.

; RUEDA, MARTA P.

; ARAUJO, PUBLIO A.

; PALOMO, LUCAS

; ITURRE, MARTA C.

; BERGALLO, HELENA GODOY

; MAGNUSSON, WILLIAM E.

Anais da Academia Brasileira de Ciências

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2021, Volumen 93 Nº 2 elocation e20201604

Resumen: En | Texto: En | PDF: En

Abstract The Program for Biodiversity Research (PPBio) is an innovative program designed to integrate all biodiversity research stakeholders. Operating since 2004, it has installed long-term ecological research sites throughout Brazil and its logic has been applied in some other southern-hemisphere countries. The program supports all aspects of research necessary to understand biodiversity and the processes that affect it. There are presently 161 sampling sites (see some of them at Supplementary Appendix), most of which use a standardized methodology that allows comparisons across biomes and through time. To date, there are about 1200 publications associated with PPBio that cover topics ranging from natural history to genetics and species distributions. Most of the field data and metadata are available through PPBio web sites or DataONE. Metadata is available for researchers that intend to explore the different faces of Brazilian biodiversity spatio-temporal variation, as well as for managers intending to improve conservation strategies. The Program also fostered, directly and indirectly, local technical capacity building, and supported the training of hundreds of undergraduate and graduate students. The main challenge is maintaining the long-term funding necessary to understand biodiversity patterns and processes under pressure from global environmental changes.

https://doi.org/10.1590/0001-3765202120201604 1034 descargas

15.

Serological evidence of Rickettsia in horses from a semi-arid Brazilian region
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Farias, Ila Ferreira

; Souza, Eline Almeida Rodrigues de

; Serpa, Maria Carolina de Azevedo

; Palha, Flávio Silva

; Oliveira, Glauber Meneses Barboza de

; Labruna, Marcelo Bahia

; Horta, Mauricio Claudio

Revista Brasileira de Parasitologia Veterinária

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2021, Volumen 30 Nº 2 elocation e026220

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo A Febre Maculosa Brasileira (FMB) é uma importante zoonose causada por Rickettsia rickettsii, sendo seu vetor o Amblyomma sculptum. Cavalos são os principais hospedeiros desse carrapato e considerados sentinelas eficientes para detectar a circulação de Rickettsia. O objetivo deste estudo foi detectar anticorpos reativos a antígenos de R. rickettsii em cavalos de uma área não-endêmica na região Centro-Norte da Bahia, Brasil. Foram coletadas amostras de sangue e carrapatos de 70 cavalos nos municípios de Senhor do Bonfim, Antônio Gonçalves, Pindobaçu e Campo Formoso. Os soros obtidos foram testados pela reação de imunofluorescência indireta para detectar anticorpos anti-Rickettsia. No geral, 5,7% (4/70) dos cavalos reagiram sorologicamente para antígenos de R. rickettsii. Os carrapatos foram coletados em 18,6% (13/70) dos cavalos e foram identificados como A. sculptum e Dermacentor nitens. Embora seja uma área não endêmica, foram observados animais soropositivos, sugerindo a circulação de riquétsias na região estudada. Este é o primeiro estudo de levantamento sorológico desse agente em cavalos do Centro-Norte da Bahia. São necessários mais estudos para compreender a epidemiologia da FMB nesses locais.

Abstract Brazilian spotted fever (BSF) is a common tick-borne disease caused by Rickettsia rickettsii. Horses are the primary hosts of the main vector, Amblyomma sculptum, and are considered efficient sentinels for circulation of Rickettsia. Therefore, the aim of this study was to detect antibodies reactive to R. rickettsii antigens in horses from a non-endemic area in the north-central region of Bahia state, Brazil. Blood samples and ticks were collected from 70 horses from the municipalities of Senhor do Bonfim, Antônio Gonçalves, Pindobaçu, and Campo Formoso. The sera obtained were tested by an indirect immunofluorescence assay to detect anti-Rickettsia antibodies. Overall, 5.7% (4/70) of the horses reacted to R. rickettsii antigens. Ticks were collected from 18.6% (13/70) of the horses and were identified as A. sculptum and Dermacentor nitens. Despite being a non-endemic area, seropositive animals were identified in our study, suggesting circulation of rickettsial agents in the region. This is the first serological survey of this agent in horses from the north-central region of Bahia, and further studies are needed to understand the epidemiology of BSF in these locations.

https://doi.org/10.1590/s1984-29612021011 276 descargas

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Ud. puede enriquecer su búsqueda de una forma muy sencilla. Use los índices de búsqueda combinados con los conectores (AND u OR) para especificar su busca.

Por ejemplo, si desea buscar artículos acerca de
casos de dengue en el Brasil en 2015, use:
ti:dengue and publication_year:2015 and aff_country:Brasil

A continuación se muestra la lista completa de los índices de búsqueda que se pueden utilizar:

Cód. del Índice	Elemento
ti	título del articulo
au	autor
kw	palabras clave del artículo
subject	asunto (palabras del título, resumen y palabras clave)
ab	resumen
ta	título abreviado de la revista (ej. Cad. Saúde Pública)
journal_title	título completo de la revista (ej. Cadernos de Saúde Pública)
la	código de idioma de publicación (ej. pt - Portugués, es - Español)
type	tipo de documento
pid	identificador de la publicación
publication_year	año de publicación del artículo
sponsor	financiador
aff_country	código del país de la afiliación del autor
aff_institution	institución de filiación del autor
volume	volumen del artículo
issue	número del artículo
elocation	elocation
doi	número DOI
issn	ISSN de la revista
in	código de la colección SciELO (ej. scl - Brasil, col - Colômbia)
use_license	código de licencia de uso del artículo

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