Esta pesquisa teve como objetivo analisar protocolos com registros do jogo Dominó das 4cores, para investigar diferentes formas de resolução propostas por 46 crianças, de idade entre sete e onze anos, que frequentavam atividades em um contexto de oficinas de jogos. Para a coleta de dados, os participantes resolveram situações-problema e as informações foram obtidas por meio da análise dos protocolos. A partir desta análise, foi possível caracterizar os modos de resolução em termos de respeito às regras, estratégias, erros e acertos. Os resultados indicaram que: 1- os sujeitos jogaram de acordo com as regras; 2- atuaram regularmente por ensaio-e-erro; 3- houve alta frequência de erros percebidos a posteriori. Como consequência, foi possível propor intervenções para desencadear a observação das ações e a construção de novos procedimentos e atitudes favoráveis ao jogar, assim como ao desenvolvimento e à aprendizagem escolar.

In this study we aim at analysing protocols of a game named 4 Colours Domino, in order to investigate different forms of solution proposed by 46 participants aged 7 to 11 years old. All of them took part in a playing games context. To collect data children faced problem-solving situations and the protocols were analysed. Considering this analysis, it was possible to characterize the different modes of solution in terms of rules, strategies, success and mistakes. Results showed that: (a) participants played according to the rules; (b) in general, they acted by chance; (c) most of them noticed the mistakes only after they were made. As a consequence, it was possible to define adequate interventions to help students observe their actions and build up new procedures to surpass mistakes, contributing to the development and learning processes.

Esta investigación tuvo como objetivo analizar protocolos con registros del juego Dominó de los 4 colores, para investigar diferentes formas de resolución propuestas por 46 niños, de edad entre siete y once años, que frecuentaban actividades en un contexto de talleres de juegos. Para la recolecta de datos, los participantes solucionaron situaciones-problema y las informaciones fueron obtenidas por intermedio del análisis de los protocolos. Tras este análisis, fue posible caracterizar los modos de resolución en términos de respeto a las reglas, estrategias, errores y aciertos. Los resultados indicaron que: 1- los sujetos jugaron de acuerdo con las reglas; 2- actuaron regularmente por ensayo-y-error; 3- hubo alta frecuencia de erros percibidos a posteriori. Como consecuencia, fue posible proponer intervenciones para desencadenar la observación de las acciones y la construcción de nuevos procedimientos y actitudes favorables al jugar, así como al desarrollo y al aprendizaje escolar.

Corynebacterium pseudodiphtheriticum is a well-known human pathogen that mainly causes respiratory disease and is associated with high mortality in compromised hosts. Little is known about the virulence factors and pathogenesis of C. pseudodiphtheriticum. In this study, cultured human epithelial (HEp-2) cells were used to analyse the adherence pattern, internalisation and intracellular survival of the ATCC 10700 type strain and two additional clinical isolates. These microorganisms exhibited an aggregative adherence-like pattern to HEp-2 cells characterised by clumps of bacteria with a "stacked-brick" appearance. The differences in the ability of these microorganisms to invade and survive within HEp-2 cells and replicate in the extracellular environment up to 24 h post infection were evaluated. The fluorescent actin staining test demonstrated that actin polymerisation is involved in the internalisation of the C. pseudodiphtheriticum strains. The depolymerisation of microfilaments by cytochalasin E significantly reduced the internalisation of C. pseudodiphtheriticum by HEp-2 cells. Bacterial internalisation and cytoskeletal rearrangement seemed to be partially triggered by the activation of tyrosine kinase activity. Although C. pseudodiphtheriticum strains did not demonstrate an ability to replicate intracellularly, HEp-2 cells were unable to fully clear the pathogen within 24 h. These characteristics may explain how some C. pseudodiphtheriticum strains cause severe infection in human patients.