Abstract: Sugarcane cultivar RB961552 was developed by the Sugarcane Breeding Program of the Federal University of Alagoas, a member university of RIDESA. It was selected for being highly productive, responsive to fertigation and for the high leaf biomass and excellent inter-row closure. It is resistant to brown and orange rust and moderately resistant to leaf scald and smut diseases.

Abstract Sugarcane cultivar RB0442 has a high yield, late maturation and good drought tolerance. It is recommended for planting in restrictive environments, mainly with water-deficient soils, and is resistant to the major sugarcane diseases of the Northeastern region of Brazil.

Resumo Objetivo Verificar o valor preditivo do escore de Lee para a mortalidade no primeiro ano pós operatório de fraturas de fêmur proximal. O estudo também avaliou a capacidade preditiva isolada de outras variáveis. Método Uma amostra de 422 pacientes com fraturas do fêmur proximal submetidos a cirurgia foi avaliada neste estudo. Os dados foram coletados por meio de revisão de prontuários, consultas presenciais e contatos telefônicos. Resultados O escore de Lee foi aplicado em 99,3% dos pacientes com fraturas de fêmur proximal submetidos a tratamento cirúrgico. A taxa de mortalidade da amostra foi de 22%, a maioria classificada como classe I de risco. O escore de Lee não apresentou associação significativa com a mortalidade (p = 0,515). Os valores elevados de creatinina sérica (p = 0,001) e a idade (p = 0,000) estiveram diretamente associados com o desfecho de morte. Conclusões O escore de Lee não é preditivo para amortalidade emumperíodo de um ano após cirurgia de fraturas de fêmur proximal; entretanto, observou-se significância estatística entre a idade e a dosagem sérica da creatinina, isoladamente, com o desfecho de morte.

Abstract Objective To verify the predictive value of the Lee score for mortality in a one-year period after proximal femur fracture surgery. The present study also evaluated the isolated predictive capacity of other variables. Methods A sample of 422 patients with surgically-treated proximal femur fractures was evaluated. Data was collected through a review of medical records, appointments, and contact by telephone. Results The Lee score was applied to 99.3% of the patients with proximal femur fractures submitted to surgical treatment. Themortality rate was of 22% of the sample, and the majority were classified as class I risk. The Lee score had no significant association with mortality (p = 0.515). High levels of serum creatinine (p = 0.001) and age (p = 0.000) were directly associated with death. Conclusion The Lee score was not predictive of mortality in a one-year period after proximal femur fracture surgery; however, a statistical significance was observed between age and serum creatinine levels, considered separately, and death.

RESUMO OBJETIVO: Avaliar o resultado pós-operatório dos pacientes com fratura do tornozelo pelo mecanismo de supinação-rotação externa que foram submetidos a retirada do parafuso transindesmoidal (PT). MÉTODOS: Estudo de coorte retrospectivo que avaliou os resultados pós-operatórios tardios de 35 pacientes operados entre janeiro de 2013 e junho de 2015. Foram incluídos pacientes submetidos ao tratamento da ruptura da sindesmose tibiofibular distal com fixação com PT e que não apresentavam lesões cirúrgicas concomitantes em outros sítios que não o tornozelo. Pacientes que não foram devidamente acompanhados no pós-operatório foram excluídos. RESULTADOS: Não houve diferença estatisticamente significativa nos desfechos avaliados entre os pacientes que tiveram o PT removido e os que permaneceram com o PT. CONCLUSÃO: A retirada do PT não alterou significativamente o resultado clínico dos pacientes tratados cirurgicamente com PT por fraturas do tipo supinação-rotação externa.

ABSTRACT OBJECTIVE: To evaluate the postoperative results of patients with supination-external rotation ankle fractures who underwent syndesmotic screw (SS) removal. METHODS: Retrospective cohort study assessing the late postoperative results of 35 patients operated from January 2013 to June 2015. Patients undergoing treatment of rupture of the distal tibiofibular syndesmosis with SS fixation and who did not have any concomitant surgical injuries in sites other than the ankle were included. Patients who did not complete appropriate follow-up after surgery were excluded from the study. RESULTS: There was no statistical significant difference in the evaluated outcomes among the patients who had their SS removed and those who remained with the SS. CONCLUSION: SS removal did not significantly alter the clinical results of patients surgically treated with SS for supination-external rotation fractures.

RESUMO OBJETIVO: Avaliar os resultados clínicos do tratamento cirúrgico das fraturas intra-articulares do calcâneo (TCFIAC) e comparar o uso de placa própria para calcâneo (PPC) e placa reta (PR) . MÉTODOS: Estudo retrospectivo que avaliou o resultado pós-operatório de 25 pacientes entre 2013 e 2015. Foram incluídos pacientes submetidos ao TCFIAC e que não apresentavam lesões cirúrgicas concomitantes. Pacientes que não foram devidamente acompanhados no pós-operatório foram excluídos da análise. RESULTADOS: A indisponibilidade da PPC em serviços com recursos limitados, associada à disponibilidade e ao menor custo da PR, pode ter sido fator de confusão no presente estudo. Contudo, não houve diferença estatística entre os resultados das fraturas tratadas com PPC ou PR. CONCLUSÃO: A inferência estatística permite concluir que, na ausência da PPC, é possível usar a PR com desfechos clínicos semelhantes.

ABSTRACT OBJECTIVE: To evaluate the clinical results of surgical treatment of intraarticular fractures of the calcaneus, comparing the use of calcaneal plate and flat plate. METHODS: This was a retrospective study assessing the postoperative results of 25 patients between 2013 and 2015. Patients undergoing surgical treatment of intraarticular fractures of the calcaneus without concomitant surgical lesions were included. Patients who did not complete appropriate follow-up after surgery were excluded from the study. RESULTS: The unavailability of calcaneal plates at resource-limited settings, associated with the availability and lower cost of flat plates, may have been a confounding factor in the present study. However, there was no statistical difference between the outcomes of fractures treated with calcaneal plates or flat plates. CONCLUSION: Statistical inference shows that, when calcaneal plates are not available, it is possible to use flat plates with similar clinical outcomes.

RB99395 cultivar was developed by the Sugarcane Breeding Program at the Federal University of Alagoas, which is part of RIDESA. In 1999, seeds were obtained from crosses between RB867515 cultivar and pollen from an unknown genotype in "Serra do Ouro" Crossing Station. The process of selection and experimentation was carried out in three Research Stations of Alagoas. RB99395 cultivar was released in Alagoas in May 2010, and has a high content of sucrose throughout the harvest period. Planting is recommended in fertile soils and in environments with no water deficiency, a condition which results in high agricultural yields. It is tolerant most diseases that occur in Northeast, Brazil.

OBJETIVOS: avaliar os fatores clínicos, patológicos e imunoistoquímicos podem ser indicativos de comprometimento metastático de outros linfonodos em pacientes com carcinoma de mama submetidas à biópsia do linfonodo sentinela (BLNS). MÉTODOS: estudo retrospectivo de 1.000 pacientes sucessivas com biópsia do LS, de 1998 a 2008. Foram avaliados: idade, tamanho do tumor, grau histológico, invasão angiolinfática, o status de receptores hormonais e HER-2, o tamanho da metástase e número de LS positivos. As associações entre as características dos tumores e os tipos de metástases foram avaliadas com testes de razão de verossimilhança corrigida com χ2 para amostras insuficientes. RESULTADOS: a idade média foi 57,6 anos, e o tamanho médio do tumor foi de 1,85 cm. Um total de 72,2% LS foram negativos e 27,8% foram positivos, mas em 61,9% dos casos, o LS foi o único positivo; com 78,4% de macrometástases, 17,3% de micrometástases e 4,3% de células tumorais isoladas (CTI). O tamanho do tumor foi fator preditivo de metástases em linfonodos não-sentinela. Após 54 meses de acompanhamento, não houve recidivas em pacientes com CTI, no grupo de micrometástases houve uma recorrência local e duas sistêmicas, e no grupo de macrometástases ocorreram quatro locais e 30 a distância. CONCLUSÕES: dos parâmetros clínicos estudados, apenas o tamanho do tumor foi correlacionado com comprometimento metastático de linfonodos axilares. O tamanho das metástases e do número de LS positivos também aumenta diretamente a possibilidade de recidiva sistêmica. As diferentes taxas de recidiva indicam que o significado biológico desses tipos de metástases é diferente e que os pacientes com metástases nos LS também podem ter diferentes riscos de comprometimento metastático de outros linfonodos axilares.

PURPOSE: to evaluate which clinical, pathological or immunohistochemical factors may be predictive of metastatic involvement of other lymph nodes in patients with breast carcinoma undergoing sentinel lymph node biopsy (SLNB). METHODS: a retrospective study carried out with 1,000 successive patients with SLNB from 1998 to 2008. Age, tumor size, histological grade, lymphovascular invasion, hormone receptor status and HER-2, size of metastasis and number of positive SLN were evaluated. The associations between the characteristics of the tumors and the types of metastases were evaluated through χ2 corrected likelihood ratio tests for insufficient samples. RESULTS: mean age was 57.6 years and mean tumor size was 1.85 cm. A total of 72.2% SLN were negative and 27.8% were positive, but in 61.9% of the cases, the SLN was the only positive one, with 78.4% having macrometastases, 17.3% micrometastases and 4.3% isolated tumor cells (CTI). Tumor size was predictive of metastases in non-sentinel lymph nodes. After 54 months of follow-up, there were no recurrences in patients with CTI, but one local recurrence and two systemic recurrences were observed in the micrometastasis group, as well as four local and 30 distant metastases in the macrometastasis group. CONCLUSIONS: among the clinical parameters studied, only tumor size was correlated with metastatic involvement in axillary lymph nodes. The size of the metastases and the number of positive SLN also directly increased the possibility of systemic recurrence. The different rates of recurrence indicate that the biological significance of these types of metastases is different and that patients with SLN metastases may also have different risks of metastatic involvement of other axillary lymph nodes.

Um procedimento detalhado para validação intralaboratorial de métodos, incluindo delineamento experimental, estatísticas e avaliação de premissas foi proposto e aplicado à validação de um método para ensaio de avermectinas em leite bovino por cromatografia líquida de alta eficiência com detector de fluorescência. Foram realizados ensaios com curvas de solventes e de matriz, amostras de leite bovino brancas e adicionadas. As premissas relacionadas às estatísticas empregadas foram avaliadas e confirmadas. Linearidade foi obtida entre 25 e 150 ng.mL-1. Não foram observados efeitos de matriz significativos nesta faixa. As médias de recuperação aparente variaram de 87,2 a 101,4%. Desvios padrão relativos sob condições de repetitividade estiveram entre 3,3 e 11,2%, enquanto os desvios padrão relativos de reprodutibilidade parcial foram de 7,4 a 14,7%. Estes resultados indicaram exatidão e precisão do método entre 10 e 30 µg.L-1 para os quatro analitos estudados. Os limites de detecção e quantificação experimentais foram 5 e 10 µg.L-1, respectivamente. Limites de decisão (12,6 a 13,7 µg.L-1) e capacidades de detecção (15,1 a 17,4 µg.L-1) foram estimados, assumindo um limite máximo de resíduo de 10 µg.L-1.

A detailed procedure for in-house validation of methods, including experimental design, statistics and evaluation of assumptions, was proposed and applied to the validation of a method for screening avermectins in cow's milk by high performance liquid chromatography coupled with fluorescence detection. Assays were performed with solvent and matrix-matched calibration curves, milk blanks and spiked samples. The assumptions about the statistics employed were evaluated and confirmed. Linearity was achieved between 25 and 150 ng.mL-1. No significant matrix effects were observed in this range. Mean apparent recoveries varied from 87.2 to 101.4%. The relative standard deviations under conditions of repeatability ranged from 3.3 to 11.2%, while the relative standard deviations of partial reproducibility varied from 7.4 to 14.7%. These results indicated exactness and precision of the method between 10 and 30 µg.L-1 for the four analytes studied. The experimental limits of detection and quantification were 5 and 10 µg.L-1, respectively. Decision limits (12.6 to 13.7 µg.L-1) and detection capabilities (15.1 to 17.4 µg.L-1) were estimated, assuming a maximum residue level of 10 µg.L-1.

OBJETIVO: Comparar o padrão hemodinâmico, angiográfico e a morfologia da lesão aterosclerótica em diabéticas e não-diabéticas com angina instável ou infarto agudo do miocárdio sem supradesnivelamento do segmento ST (AI/IAMSS). MÉTODOS: Dois hemodinamicistas determinaram a presença de lesão aterosclerótica grave, definida como > 70%, a morfologia da placa, de acordo com a classificação da American Heart Association, a presença de circulação colateral e as pressões ventriculares e aórticas. A fração de ejeção foi calculada pela angiografia ou pelo ecocardiograma. RESULTADOS: Em oito anos e meio, foram realizados 645 coronariografias em mulheres com AI/IAMSS. Foram analisadas 593 pacientes (215 diabéticas - 36%). Este grupo diferiu das não-diabéticas nos seguintes aspectos: idade mais alta (61 ± 10,6 x 58,1 ± 11,4), prevalência maior de mulheres pós-menopausa e menor prevalência de tabagismo. Lesão grave em três vasos foi significativamente mais freqüente nas pacientes diabéticas (28% x 10%), assim como vasos totalmente ocluídos: 51 (23%) x 54 (14.3%), p < 0.005.Fração de ejeção < 50% foi mais comum nas diabéticas. CONCLUSÃO: Estes achados confirmam o acometimento difuso da doença aterosclerótica em pacientes diabéticas, assim como maior deterioração da função ventricular, que pode estar relacionada ao pior prognóstico dessa população em curto e em longo prazo.

OBJECTIVE: Compare hemodynamic and angiographic patterns, as well as atherosclerotic lesion morphology, in diabetic and non-diabetic females with unstable angina or non-ST-segment-elevation myocardial infarction (UA/NSTEMI). METHODS: Two interventional cardiologists determined the presence of severe atherosclerotic lesion, defined as those > 70%; plaque morphology, according to the American Heart Association classification; collateral circulation; plus ventricular and aortic pressures. Ejection fraction was calculated by angiography or echocardiography. RESULTS: During eight and a half years, 645 coronary angiographies were performed in women with UA/NSTEMI. In the present study, 593 female patients were assessed (215 diabetic - 36%). This group differed from the non-diabetic in the following aspects: older age (61 ± 10.6 x 58.1 ± 11.4), higher prevalence of postmenopausal women and lower prevalence of the smoking habit. Severe three-vessel disease was significantly more frequent in diabetic patients (28% x 10%), as well as totally occluded vessels: 51 (23%) x 54 (14.3%), p < 0.005. Additionally, ejection fraction < 50% was more common in diabetic patients. CONCLUSION: These findings confirm the diffuse pattern of atherosclerotic disease in diabetic patients, as well a greater deterioration of ventricular function, which may be associated to the poorer prognosis seen in this population both in the short- and long-term.

A leishmaniose visceral no Brasil estava inicialmente associada a áreas rurais, mas devido às diversas alterações no ambiente como, desmatamentos, urbanização e intenso processo migratório, ocorreu a expansão das áreas endêmicas, levando à urbanização da doença, principalmente nas regiões Sudeste e Centro Oeste do país. No município de Montes Claros, situado ao norte de Minas Gerais, foi feito um estudo para verificação da situação da LV. No ano de 2002 foi realizado inquérito sorológico canino e no período de setembro de 2002 a agosto de 2003 foi feito levantamento entomológico, utilizando armadilhas luminosas de CDC. A prevalência da LV canina apresentou taxa média de infecção em torno de 5%. A fauna de flebotomíneos estimada foi de 16 espécies, totalizando 1043 exemplares. Lutzomyia longipalpis foi a espécie predominante com 74%, o que sugere a sua participação na transmissão de LV em Montes Claros.

Visceral leishmaniasis in Brazil was initially associated with rural areas. However, due to several environmental modifications such as deforestation, urbanization and intense migratory processes, there has been an expansion of endemic areas, leading to urbanization of the disease, mainly in the central and northeastern regions of Brazil. In the municipality of Montes Claros, located in the north of the state of Minas Gerais, an epidemiological survey on VL was carried out. A canine serological inquiry was carried out in 2002 and an entomological survey, using luminous CDC traps, was performed from September 2002 to August 2003. Canine VL prevalence showed an average infection rate of approximately 5%. An estimated 16 species comprised the phlebotomine sand fly fauna, based on a total of 1043 specimens. The predominant species was Lutzomyia longipalpis with a rate of 74%, suggesting its participation in the transmission of VL in the municipality of Montes Claros.

A study of the phlebotomine sand fly fauna was carried out in an endemic area of American visceral leishmaniasis (AVL) in the municipality of Porteirinha, in the Brazilian state of Minas Gerais. Captures were performed with CDC light traps in 7 districts, 5 days per month, during 2 consecutive years (January 2000 to December 2001). A total of 3240 sand flies were captured and identified. Sixteen species were found, among which 15 belonged to the genus Lutzomyia and one to the genus Brumptomyia. Lutzomyia longipalpis, a proven vector of AVL, was the predominant species (71.85%) throughout the time period. The interference of climatic factors (temperature, humidity, and rainfall) over the populational dynamics of the sand flies was determined. Statistical analysis of the data showed a significant correlation among the number of phlebotomine sand flies collected, rainfall, and humidity, whereas the effect of temperature was negligible, in that particular region. The amount of collected phlebotomine, the number of human cases, and the prevalence of canine AVL in the districts of Porteirinha are discussed.

Proteínas imunogênicas da vacina polivalente de promastigotas mortas de leishmanias (Leishvacin®) produzida pela Biobrás Bioquímica do Brasil, Montes Claros, Minas Gerais, Brasil foram identificadas e purificadas por eletroforese em gel de poliacrilamida e eletroeluição. Camundongos C57BL/10 foram vacinados com proteínas de pesos moleculares estimados em 42, 46, 63, 66, 73, 87, 97 e 160kDa em três doses de 30µg de cada proteína combinada com 250µg de Corynebacteriumparvum em intervalos de 15 dias e desafiados com uma infecção desafio de 10(5) promastigotas infectantes de Leishmania (Leishmania) amazonensis na base da cauda. Foram avaliadas a habilidade dessas proteínas em induzir resposta imune e proteção dos animais vacinados após a infecção desafio. Nenhuma diferença estatística foi observada nos níveis de IFN-g nos grupos vacinados em comparação ao grupo controle. Proteção de 28,57; 42,86; 57,14; 42,86; 42,86, 57,14; 42,86; 57,14% foi demonstrado para cada proteína.

Immunogenic proteins from nonliving promastigote polyvalent Leishmania vaccine against American tegumentary leishmaniasis (Leishvacin®), produced by Biobrás (Biochemistry of Brazil ), Montes Claros, State of Minas Gerais, Brazil, were identified and purified by polyacrylamide electrophoresis gel and electroelution. C57BL/10 mice were vaccinated with proteins with estimated molecular weights of 42, 46, 63, 66, 73, 87, 97, and 160kDa in three doses of 30µg of each protein at 15-day intervals combined with 250µg of Corynebacterium parvum followed by a challenge infection with 10(5) infective promastigotes from Leishmania (Leishmania) amazonensis. The ability of these proteins to induce immune response and protection was analyzed. No statistical difference was observed in the level of IFN-g induced by proteins in vaccinated groups in comparison with control groups. Six months after challenge infection, protection levels of 28.57; 42.86; 57.14; 42.86; 42.86, 57.14; 42.86 and 57.14% were demonstrated for each purified protein.

Evaluation of DDT insecticide used to sand flies control in domiciles and domestic animal shelters in an endemic area of cutaneous leishmaniasis. Collections of sand flies were made with Falcão traps in houses, in one pigsty and in one tree in Jussara Farm, municipality of Jussara, State of Paraná, Brazil, between 10 p.m. and 2 a.m. during 7 days from March 24 to April 1, 1992. In May, 1992 DDT insecticide (Dicloro Difenil Tricloro Etano) (1 g/square meter of area) was applied internally and externally on houses and in pigsty. After this, new collections of sand flies were made from July 1992 to February 1993, once a month, with Falcão traps installed in the same hour and conditions mentioned above. Before DDT insecticide application 12,960 sand flies were collected (Hourly Average=463) and it was observed the prevalence (61,8%). of Lutzomyia (Nyssomyia) whitmani (Antunes & Coutinho, 1939) After the DDT application only 589 sand flies were collected (Hourly Average=18,4) and also L. whitmani predominated again (90,8%). The decrease of sand flies number after the DDT insecticide application in domiciles and in pigsty prove that this measure can help cutaneous leishmaniasis control in Northern Paraná.