Objective: To record cases reported as classical dengue fever and deaths in the southern region of Brazil between 2001 and 2017. Method: Descriptive-retrospective study using secondary data from DATASUS/ SINAN and State Departments, analyzed through descriptive statistics. Results: An increase in the percentage of cases of the disease in the three states was observed in the three Brazilian southern states: in Paraná (PR) the percentage of autochthonous cases reached 94% in the last four years; in Santa Catarina (SC), the percentage was 95%, and in Rio Grande do Sul (RS), 83%, respectively, in 2015 and 2016. There was an increase in the number of deaths by severe dengue (dengue hemorrhagic fever), mainly in PR; in 2016, the first death caused by dengue was observed in SC; and an increase of more than 90% in the cases of dengue among adults in the last two years in Rio Grande do Sul. Conclusion: Despite the fact the Southern region of Brazil has the lowest incidence of dengue, special attention should be paid by health agencies to the situation in the southern states due to the increase in the number of cases and deaths caused by dengue.

ABSTRACT Objetivo: inventariar casos notificados por dengue clásica y óbitos en la región Sur de Brasil entre 2001 y 2017. Método: estudio descriptivo retrospectivo con base en datos secundarios del DATASUS/SINAN y de las Secretarías de Salud, analizados por medio de estadística descriptiva. Resultados: se observó crecimiento de los casos en los tres estados: Paraná (PR) tuvo elevación de casos autóctonos para 94% en los últimos cuatro años, Santa Catarina (SC) para 95%, y Rio Grande do Sul (RS) para 83%, respectivamente, en 2015 y 2016. Se observó todavía creciente aumento de óbitos por dengue hemorrágico, principalmente en PR; en 2016; el primero óbito en SC; y crecimiento en los últimos dos años en RS, más de 90% en adultos. Conclusión: la región necesita atención de los organismos de salud, a causa del crecimiento de casos y óbitos por dengue, a pesar de presentar la menor incidencia de la enfermedad del país. Este estudio contribuyó para el conocimiento del escenario actual y de la evolución de la dengue en la región Sur de Brasil.

Objetivo: inventariar casos notificados por dengue clássica e óbitos na região Sul do Brasil entre 2001 e 2017. Método: estudo descritivo-retrospectivo a partir de dados secundários do DATASUS/SINAN e das Secretarias de Saúde, analisados através de estatística descritiva. Resultados: observou-se aumento dos casos nos três estados: Paraná (PR) elevou casos autóctones para 94% nos últimos quatro anos, Santa Catarina (SC) para 95%, e Rio Grande do Sul (RS) para 83%, respectivamente, em 2015 e 2016. Observou-se crescente aumento de óbitos por dengue hemorrágica, principalmente no PR; em 2016, o primeiro óbito em SC; e aumento nos últimos dois anos no RS, mais de 90% em adultos. Conclusão: a região requer atenção dos órgãos de saúde, devido ao aumento de casos e óbitos por dengue, embora apresente a menor incidência da doença do país. Este estudo contribuiu para o conhecimento do cenário atual e da evolução da dengue na região Sul do Brasil.

Human adenoviruses (HAdV), members of the Adenoviridae family, are excreted through the fecal route and may be present in the feces of humans consuming contaminated food or water. The presence of HAdV from different serotypes in the feces of healthy individuals was already reported using conventional polymerase chain reaction; however, real-time PCR (qPCR) may reveal not only the rates of detection as well as demonstrate the viral loads excreted by healthy persons. Aiming to identify and characterize the presence of adenoviruses in stool samples, 147 fecal samples from patients with no records of diarrhea were analyzed (74 from winter season and 73 from summer) by Real-Time PCR (qPCR) assay and conventional PCR. HAdV genome was present in 43.8% (32/73) of stools samples collected during summer season and 21.6% (16/74) during winter. The rate of detection of genomic copies (gc) ranged from 4.04×102 to 6.72×105gc/g of feces among the 147 samples analyzed, of which the ranged of genomic copies of DNA HAdV was major in summer. All samples were negative when tested for rotaviruses (RV) and noroviruses (NoV) by PCR conventional and qPCR respectively. HAdV is excreted constantly by infected individuals in the absence of clinical signs and the occurrence may vary seasonally.

O presente trabalho objetivou verificar a ocorrência de fungos produtores de micotoxinas, especialmente dos gêneros Aspergillus, Fusarium e Penicillium, em granola comercializada em Porto Alegre, uma vez que este alimento vem apresentando crescente consumo. Estes fungos filamentosos são evidenciados freqüentemente em cereais, os quais apresentam grande incidência em produtos à base de granola. Amostras de granola foram adquiridas no Mercado Público de Porto Alegre, no período de agosto de 2004 a abril de 2005, nas quatro estações do ano. A coleta baseou-se em quatro amostras de diferentes procedências, duas das quais são comercializadas embaladas e lacradas e duas comercializadas a granel. As análises seguiram os procedimentos descritos pelo Compendium of Methods for Examination of Foods. A identificação dos fungos baseou-se na morfologia macroscópica e microscópica, com auxílio de chaves de identificação. Os resultados evidenciaram a presença dos três gêneros fúngicos, com predominância de Aspergillus em três estações. No verão houve maior desenvolvimento micelial e no outono, menor crescimento de fungos. Os resultados sugerem maior controle e fiscalização, visando eliminar qualquer ocorrência de microrganismos produtores de toxinas em granola.

The following work, had the purpose to verify the occurrence of fungi which produce mycotoxin, specially the Aspergillus, Fusarium and Penicillium genus in the granola commercialized in Porto Alegre, once that this food is having an increasing consumption.These fibered fungi are certified frequently in cereals, which are found in the granola (a mix of grains, nuts and dried fruits, sometimes coated with oil and honey, eaten for breakfast or as a snack). Granola samples were acquired in the Public Market of Porto Alegre since August 2004 until April 2005, during the four seasons of the year. The collection was based on four samples of different origins, two of which are commercialized packed and sealed up, and the other two commercialized in bulk. The analyses had followed the described procedures for Compendium of Methods for Examination of Foods. The fungi identification was based on the macroscopic and microscopic morphology, with the assistance of identification keys. The results certified the presence of Aspergillus, Fusarium and Penicillium fungi, with the predominance of the Aspergillus genus in three seasons. In summer it had a bigger mycelium development and in autumn the lowest fungi growth. The results suggest a bigger control and inspection, seeking for eliminate any occurrence of microorganism producer of toxins in the granola.