ABSTRACT An experiment was carried out to investigate the effect of using olive cake (OC) in broiler diets, when it is supplemented with multi-enzymes or phytase enzyme. The OC was included in isocaloric, isonitorgneous diets at 5 and 10% levels and fed to broilers from 1-28 days of age. Experimental diets were fed with or without either of the two enzymes: galzym or phytase. This resulted in 3 OC levels (0, 5, 10%) × 3 enzyme supplementations (no enzyme, galzym enzyme, phytase enzyme). This included nine treatments, and each treatment was replicated eight times with seven broiler chickens each. Feed intake, feed conversion ratio, body weight gain, survival rate, dressing, inner and immune organ´s weights (compared to live body weight); and blood lipids constituents were investigated. According to the findings in this study, it could be concluded that OC is a valuable ingredient and might be included in the broiler diet up to 10% without galzym or phytase enzyme addition. Also, further studies should investigate the possibility of using higher ratios of it or mixed with another by-product in poultry diets; as a very cheap by-product. Moreover, these studies can be associated with suitable additives at different concentrations that might help to increase the utilization of olive cake or at least to keep performance equal to the control. On the other hand, it is worthwhile to follow the positive effect of phytase enzyme on cholesterol and very low density lipoprotein (VLDL) concentrations, which may relate it´s use with chicken´s health.