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The copper alloy UNS-C27200 is a binary brass, composed of 38wt%Zn, beeing one of the most used in the industry. The union of Cu-Zn alloys by conventional welding by fusion in thin plates presents several challenges. The Friction Stir Welding (FSW) process performs the union of two, or more materials, through a rotating tool that travels throughout the welding region. One the great advantages of FSW is working below the melting point of the material, which reduces the change in the microstructure and properties of the material, when compared to conventional welding processes. Due to the lack of research on FSW process in lap brass joints with thin thicknesses, the present work aims to expand these studies in order to contribute to future research. Eight conditions were evaluated, varying rotation and welding speed. In the best results by visual inspection, analyzes of microstructure, temperature, microhardness and mechanical strength were performed. The better welding results were obtained with ω=1050 rpm and ν=20 mm/min. There was reduction of approximately 50% between Stir Zone and Base metal. The mechanical strength was affected by the pin hole resulting from the process. The microstructure revealed a homogeneous mixture in the stir zone and no defects were detected.

https://doi.org/10.1590/1980-5373-mr-2022-0581

Opiliofauna (ARACHNIDA: OPILIONES) of the Atlantic Forest in the state of Paraná, Brazil
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Rubim, L. G. T.

; Pádua, T. H. R.

; Souza, A. S. B.

; Almeida, J. A. M.

; Shimamoto, C. Y.

; Barbado, N.

; Souza, M. M.

Brazilian Journal of Biology

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2023, Volume 83 elocation e274971

Abstract: En Pt | Text: En Pt | PDF: En | PDF: Pt

Abstract Opiliones are arachnids that provide different services in terrestrial ecosystems, especially in tropical forests, which justifies inventory studies to better understand the occurrence and distribution of these arthropods in Brazil, since little is known about the harvestmen fauna in Conservation Units such as in the state of Paraná. In this context, the objective of the present study was to survey the harvestmen fauna at the Iguaçu National Park (PARNA) and in the Bela Vista Biological Refuge (BVBR), from September 2021 to May 2022, using a sampling effort of 40 hours in the BVBR, and 66 hours at the PARNA Iguaçu. Ten species/morphospecies were recorded, highlighting a new occurrence for Brazil, the species Opisthoplatus vegetus. The present study reduces the information gap about Opiliones in the state, which reaffirms the importance of Conservation Units for the protection of the biota in the state of Paraná.

Resumo Opiliões são aracnídeos que prestam diferentes serviços nos ecossistemas terrestres, sobretudo nas florestas tropicais, o que justifica trabalhos de inventários para melhor conhecer a ocorrência e distribuição desses artrópodes no Brasil, pois pouco se conhece da fauna de opiliões em Unidades de Conservação no estado do Paraná. Nessa perspectiva, o objetivo do presente estudo visa inventariar a fauna de opiliões no Parque Nacional do Iguaçu (PARNA Iguaçu) e no Refúgio Biológico Bela Vista (RBBV), no período de setembro de 2021 a maio de 2022, com esforço amostral de 40 horas para RBBV e 66 horas PARNA Iguaçu. Foram registradas 10 espécies/morfoespécies, com destaque para uma nova ocorrência para o Brasil, a espécie Opisthoplatus vegetus. O presente estudo diminui a lacuna de informações sobre opiliones no estado e reafirma a importância da Unidades de Conservação para proteção da biota do Paraná.

https://doi.org/10.1590/1519-6984.274971

Different stocks of Prochilodus lineatus along the Cinzas River basin: contributions from a free-flowing tributary in the Capivara Reservoir region, Paranapanema River basin (upper Paraná River) freeflowing free flowing region upper
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Ferreira, Dhiego G.

; Galindo, Bruno A.

; Limeira, Daniel M.

; Garla, Alanna

; Marques, Ana J. C.

; Moreira, Amanda A.

; Rodrigues, Maria V. H.

; Clemente, Roberta C.

; Shimpo, Jhennifer Y.

; Martins, Gabriel A.

; Almeida, Fernanda S.

; Apolinário-Silva, Caroline

; Zanatta, Augusto S.

; Sofia, Silvia H.

; Souza-Shibatta, Lenice

Neotropical Ichthyology

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2023, Volume 21 N. 4 elocation e230080

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Barragens são umas das principais ameaças para peixes potamódromos Neotropicais, causando bloqueios de rotas migratórias e perdas de áreas de reprodução em um número crescente de sistemas de drenagem. Neste cenário, dados atuais defendem que a manutenção de tributários de fluxo livre em bacias hidrográficas fragmentadas seria uma importante estratégia na conservação de peixes migradores, tanto em situações de panmixia quanto em cenários com diferentes estoques coexistindo, tal como já relatado para Prochilodus lineatus. Este estudo analisou locos microssatélites e haplótipos mitocondriais em amostras de P. lineatus obtidas em diferentes anos ao longo da bacia do rio das Cinzas, no reservatório de Capivara (maior reservatório na calha principal do rio Paranapanema, alto rio Paraná) e no rio Tibagi, todos conectados por trechos livres, visando investigar as contribuições da bacia do rio das Cinzas na dinâmica populacional de peixes migradores na região do reservatório de Capivara. Ambos os marcadores detectaram altos níveis de diversidade genética e indicaram diferentes estoques de P. lineatus ao longo da bacia do rio das Cinzas, sugerindo alguma estruturação genética temporal, bem como a mistura destes estoques na amostra do reservatório de Capivara e de outros pontos, corroborando as relações de estoques neste reservatório e seus tributários. Neotropicais drenagem cenário coexistindo P maior Paranapanema Paraná Tibagi livres temporal pontos

Abstract Dams are one of the main threats to Neotropical potamodromous fish, causing blockages of migratory routes and loss of breeding areas in an increasing number of drainage systems. In this scenario, current data argue that the maintenance of free-flowing tributaries along fragmented watersheds would be an important strategy to the migratory fish conservation, both in panmixia situations and in scenarios with different coexisting stocks, as already reported for Prochilodus lineatus. This study analyzed microsatellite loci and mitochondrial haplotypes in samples of P. lineatus obtained in different years along the Cinzas River basin, as well as in the Capivara Reservoir (largest Reservoir in the Paranapanema main channel, upper Paraná River) and in the Tibagi River, all connected by free stretches, aiming to investigate the contributions of the Cinzas River basin to the population dynamics of migratory fish in the Capivara reservoir region. Both markers detected high genetic diversity levels and indicated different genetic stocks of P. lineatus along the Cinzas River basin, suggesting some temporal genetic structuring, as well as the mixture of these stocks in the sample from the Capivara reservoir and from other points, corroborating the relationships among stocks in this reservoir and its tributaries. systems scenario freeflowing flowing conservation P largest channel stretches region structuring points

https://doi.org/10.1590/1982-0224-2023-0080

Antiproliferative, genotoxic and mutagenic potential of synthetic chocolate food flavoring
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Frâncica, L. S.

; Gonçalves, E. V.

; Santos, A. A.

; Vicente, Y. S.

; Silva, T. S.

; Gonzalez, R. S.

; Almeida, P. M.

; Feitoza, L. L.

; Bueno, P. A. A.

; Souza, D. C.

; Peron, A. P.

Brazilian Journal of Biology

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2022, Volume 82 elocation e243628

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Os aditivos aromatizantes têm grande importância tecnológica para a indústria de alimentos. Contudo, poucas são as informações quanto as propriedades toxicológicas desses microingredientes, especialmente, em nível celular. No presente estudo avaliou-se, sobre as células meristemáticas de raízes de Allium cepa L., a toxicidade de um aditivo sintético líquido de aroma e sabor de chocolate, fabricado e amplamente comercializado em todo Brasil, e exportado para outros países da América do Sul. As concentrações de aromatizante avaliadas foram 100,00; 50,00; 25,00; 1,00; 0,50 e 0,25 µL/L, onde a maior concentração estabelecida foi cem vezes menor que a sugerida comercialmente para uso. Com base na interpretação dos resultados, a concentração 100 µL/L reduziu substancialmente a divisão celular dos meristemas nas 24 e 48 horas de exposição. As concentrações 100,00 a 0,50 µL/L demonstraram número significativo de prófases em detrimento as outras fases da divisão celular, indicando ação aneugênica, e induziram número significativo de alterações celulares, com ênfase a micronúcleos, broto nucleares e quebras cromossômicas. Nas condições de análises estabelecidas, com exceção a concentração 0,25 µL/L, o aromatizante de chocolate causou citotoxicidade, genotoxicidade e mutagenicidade aos meristemas radiculares.

Abstract Flavoring additives are of great technological importance for the food industry. However, there is little information regarding the toxicological properties of these micro-ingredients, especially at the cellular level. The present study used meristematic root cells of Allium cepa L. to evaluate the toxicity of a liquid, aroma and flavor synthetic chocolate additive, manufactured and widely marketed throughout Brazil and exported to other countries in South America. The flavoring concentrations evaluated were 100.00; 50.00; 25.00; 1.00; 0.50 and 0.25 µL/L, where the highest concentration established was one-hundred times lower than that commercially suggested for use. The concentration 100 µL/L substantially reduced cell division of meristems within 24- and 48-hours exposure. Concentrations from 100.00 to 0.50 µL/L resulted in a significant number of prophases to the detriment of the other phases of cell division, indicating an aneugenic activity, and induced a significant number of cellular changes, with emphasis on micronuclei, nuclear buds and chromosomal breaks. Under the established analysis conditions, with the exception of concentration 0.25 µL/L, the flavoring of chocolate caused cytotoxicity, genotoxicity and mutagenicity to root meristems.

https://doi.org/10.1590/1519-6984.243628 256 downloads

Predicting latent classes of drug-related problems among adolescents: secondary analysis of a cluster randomized controlled trial
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Almeida, Mireille C.

; Valente, Juliana Y.

; Sanchez, Zila M.

Brazilian Journal of Psychiatry

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aug 2021, Volume 43 N. 4 Pages 393 - 401

Abstract: En | Text: En | PDF: En

Objective: To detect latent patterns of drug-related problems and their predictors among adolescents. Methods: A three-wave randomized controlled trial was conducted among 6,391 7th and 8th graders (51.02% girls) at 72 public schools in six Brazilian cities to evaluate the #Tamojunto school-based drug prevention program. Patterns of drug use and behavior problems were identified through latent class analysis, and logistic regression analyses were used to detect predictors of high-risk and low-risk groups. Results: Two groups were found that best explained the patterns of drug use and problem behaviors: high drug use/high problems and low drug use/low problems, representing 36.7 and 63.3% of the sample, respectively. No program effect was a determinant of the risk of belonging to these groups. Any reported problem behavior in the year preceding baseline assessment increased the likelihood of belonging to the high-risk group at the 21-month follow-up by 16 times. Alcohol use at baseline and female gender increased the likelihood of belonging to the high-risk group by 70%. Conclusion: Previous behavior problems and alcohol use were the strongest predictors of higher risk, suggesting that effective earlier interventions and early screening for alcohol use and behavioral problems are needed at schools, particularly for girls. Clinical trial registration: Registro Brasileiro de Ensaios Clínicos (REBEC), RBR-4mnv5g

https://doi.org/10.1590/1516-4446-2020-1075 125 downloads

Ethnodirigid study of Medicinal plants used by the population assisted by the “Programa de Saúde da Família” (Family Health Program) in Marechal Deodoro - AL, Brazil
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Melro, J. C. Lima

; Fonseca, S. A.

; Silva Júnior, J. M.

; Franco, S. P. B.

; Souza, M. A.

; Pimentel, Y. F. C

; Bomfim, M. R. P.

; Almeida, E. M.

; Costa, J. G.

; Matos-Rocha, T. J.

; Santos, A. F.

Brazilian Journal of Biology

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jun 2020, Volume 80 N. 2 Pages 410 - 423

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Este trabalho teve como objetivo conhecer perfil diagnóstico de usuários do SUS com relação à fitoterapia. Trata-se de um estudo observacional transversal, com coleta de dados realizada no Município de Marechal Deodoro, por meio de um questionário semi-estruturado com perguntas relativas a dados socioeconômicos, utilização e conhecimentos sobre plantas medicinais e fitoterápicos e satisfação com o uso de plantas medicinais (PM) e medicamentos fitoterápicos (MF). Entre os usuários abordados, 87,08% utilizam plantas medicinais frequentemente, principalmente a erva cidreira (Melissa officinalis L.). Também foi observado que as plantas são geralmente usadas como calmante (16,05%) e na forma de chá (68,35%). 82,51% dos entrevistados afirmam ter adquirido o conhecimento sobre fitoterapia através de algum familiar e 85,56% fazem uso das PM e MF sem ter recebido algum tipo de informativo sobre o seu uso. 61% das plantas descritas pelos usuários teve seu uso e terapêutica coincidente com o citado em literatura específica. Com relação a satisfação através do tratamento com PM e MF observou-se que 56,80% estão satisfeitos. O uso de plantas medicinais por parte dos usuários é bastante frequente, principalmente pelo conhecimento acumulado ao longo de gerações. Portanto, se faz necessária maior atenção a esse aspecto de utilização, visando evitar possíveis reações adversas advindas do uso incorreto.

Abstract This study aimed to assess diagnosis of Unique Health System (SUS) users profile with regard to herbal medicine. This is a cross-sectional study with observational data collection conducted in the city of Marechal Deodoro, through a semi-structured questionnaire with questions related to socioeconomic data, use, and knowledge of medicinal plants and herbal medicines and satisfaction with the use of medicinal herbs (MH) and interactions of phytotherapeutic drugs (PD). Among the topics, 86.89% of users used herbal medicines often, especially lemon balm (Melissa officinalis L.). It was also observed that the plants are generally used as a sedative (21.74%) and as tea (87.84%). 82.32% of respondents claim to have acquired the knowledge of herbal medicine through a family member and 85.36% make use of medicinal plants without having received any information on the use of MH and PD. 61% of the plants described that coincided with the form of use and therapeutic properties found in the literature. With regard to satisfaction by treatment with MH and PD, it was found that 56.67% are satisfied. Therefore, it is necessary to pay attention to this aspect of usage, in order to avoid possible drug interactions or adverse reactions resulting from the incorrect use.

https://doi.org/10.1590/1519-6984.214039 1445 downloads

Efecto del calibre semilla (masa) en la germinación del sorgo
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Ruiz-Sánchez, Michel

; Muñoz-Hernández, Yaumara

; Guzmán, Daniel

; Velázquez-Rodríguez, Raiza

; Díaz-López, Guillermo S.

; Martinez, Alexeis Y.

; Almeida, Fernando M.

Cultivos Tropicales

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dec 2018, Volume 39 N. 4 Pages 51 - 59

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

The research was carried out at the Base Scientific Technological Unit “Los Palacios”, Cuba, with the objective of determining the effect of the size of the seed (mass) of sorghum in its germination during the dry periods of 2017 and 2018. He worked with two cultivars of sorghum CIAP 132R and ISIAP Dorado. The first experiment was developed in Petri dishes and the second in metal trays (0.70 x 0.50 x 0.08 m), which contained a Gleysol Nodular ferruginoso petroférrico. Six calibers of seeds (mass) were evaluated in the two experiments: M1 (0.015 - 0.020 g); M2 (0.021-0.025 g); M3 (0.026 - 0.030 g); M4 (0.031 - 0.035 g); M5 (0.036 - 0.040 g) and the control treatment (mixture of seed size). The germination percentage (PG) was evaluated in time, with a 24 h interval until 96 h after sowing, the germination speed index (IVG), the average number of days to germination (NMDG) and the accumulation of aerial dry mass (MSA) at 10 days after the emergency (DDE). It was found that always the highest seed size (mass) corresponded to the highest PG, IVG and lowest NMDG in both cultivars. The quality indicators PG, IVG and NMDG deteriorate less in the greater seed caliber, in addition to that, to the 10 DDE accumulated greater MSA. A strong and positive relationship was found between the PG and the MSA, which indicated a dependence on the germinative power of the seed

La investigación se realizó en la Unidad Científico Tecnológica de Base “Los Palacios”, Cuba, con el objetivo de determinar el efecto del calibre de la semilla (masa) de sorgo en su germinación, en los periodos poco lluviosos de 2017 y 2018. Se trabajó con dos cultivares de sorgo CIAP 132R y ISIAP Dorado. El primer experimento se desarrolló en placas Petri y el segundo en bandejas metálicas (0,70 x 0,50 x 0,08 m), las cuales contenían un suelo Gleysol Nodular ferruginoso petroférrico. Se evaluaron seis calibres de semillas (masa): M1 (0,015-0,020 g); M2 (0,021-0,025 g); M3 (0,026-0,030 g); M4 (0,031-0,035 g); M5 (0,036-0,040 g) y un tratamiento testigo (mezcla de calibre de semillas). Se evaluó el porcentaje de germinación (PG) en el tiempo, con un intervalo de 24 horas hasta las 96 horas después de la siembra, el índice de velocidad de germinación (IVG), el número medio de días a la germinación (NMDG) y la acumulación de masa seca aérea (MSA) a los 10 días después de la emergencia (DDE). Se encontró que siempre el mayor calibre semilla (masa) se correspondió con el mayor PG, IVG y menor NMDG en ambos cultivares. Los indicadores de calidad PG, IVG y NMDG se deterioran menos en el mayor calibre semilla, además de que, a los 10 DDE acumularon mayor MSA. Se encontró una relación fuerte y positiva entre el PG y la MSA, que indicó una dependencia del poder germinativo de la semilla

Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations
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Leal, Elida A.

; Moreira, Josimar D.

; Nunes, Fernanda F.

; Souza, Larissa R.

; Martins, Janaina M.

; Toledo, Vicente P.C.

; Almeida, Alzira M.P.

; Guimarães, Tania M.P.

Brazilian Journal of Infectious Diseases

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dec 2017, Volume 21 N. 6 Pages 620 - 626

Abstract: En | Text: En | PDF: En

ABSTRACT Objectives: The plague, which is an infectious disease caused by Yersinia pestis, still threatens many populations in several countries. The worldwide increase in human plague cases and the potential use of the bacteria as a biological weapon reinforce the need to study the immunity that is induced by potential vaccine candidates. To determine the immunogenicity of antigenic preparations based on the F1 protein and the total extract from Y. pestis, we assessed the role of these antigens in inducing an immune response. Methods: The immunogenicity of antigenic preparations based on the Y. pestis (YP) total extract and the Y. pestis fraction 1 capsular antigen protein (F1) was determined in Swiss-Webster mice immunized with 40 µg or 20 µg for each preparation. Immunophenotyping was performed by flow cytometry. Results: Animals immunized with the YP total extract did not elicit detectable anti-F1 antibodies (Ab) in the hemaglutination/inhibition (HA/HI) test. Animals immunized with 40 µg or 20 µg of the F1 protein produced anti-F1 Abs, with titres ranging from 1/16 to 1/8132. The average of CD3+-CD4+ and CD3+-CD8+ T cells did not differ significantly between the groups. Neither YP total extract nor F1 protein induced a significant expression of IFN-γ and IL-10 in CD4+ T lymphocytes. In addition, F1 failed to induce IFN-γ expression in CD8+ T cells, unlike the YP total extract. Conclusion: The results showed that F1 protein is not an immunogenic T cell antigen, although the YP total extract (40 µg dose) favoured CD8+ T cell-mediated cellular immunity.

https://doi.org/10.1016/j.bjid.2017.09.001 697 downloads

Adjuvant radiotherapy for the primary treatment of adrenocortical carcinoma: are we offering the best?
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Srougi, Victor

; Bessa Junior, Jose

; Tanno, Fabio Y.

; Ferreira, Amanda M.

; Hoff, Ana O.

; Bezerra, João E.

; Almeida, Cristiane M.

; Almeida, Madson Q.

; Mendonça, Berenice B.

; Nahas, William C.

; Chambô, Jose L.

; Srougi, Miguel

; Fragoso, Maria C. B. V.

International braz j urol

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oct 2017, Volume 43 N. 5 Pages 841 - 848

Abstract: En | Text: En | PDF: En

ABSTRACT Purpose: To evaluate the role of ARDT after surgical resection of ACC. Materials and Methods: Records of patients from our institutional ACC database were retrospectively assessed. A paired comparison analysis was used to evaluate the oncological outcomes between patients treated with surgery followed by ARDT or surgery only (control). The endpoints were LRFS, RFS, and OS. A systematic review of the literature and meta-analysis was also performed to evaluate local recurrence of ACC when ARDT was used. Results: Ten patients were included in each Group. The median follow-up times were 32 months and 35 months for the ARDT and control Groups, respectively. The results for LRFS (p=0.11), RFS (p=0.92), and OS (p=0.47) were similar among subsets. The mean time to present with local recurrence was significantly longer in the ARDT group compared with the control Group (419±206 days vs. 181±86 days, respectively; p=0.03). ARDT was well tolerated by the patients; there were no reports of late toxicity. The meta-analysis, which included four retrospective series, revealed that ARDT had a protective effect on LRFS (HR=0.4; CI=0.17-0.94). Conclusions: ARDT may reduce the chance and prolong the time to ACC local recurrence. However, there were no benefits for disease recurrence control or overall survival for patients who underwent this complementary therapy.

https://doi.org/10.1590/s1677-5538.ibju.2017.0095 652 downloads

10.

3rd Guideline for Perioperative Cardiovascular Evaluation of the Brazilian Society of Cardiology
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Gualandro, Danielle Menosi

; Yu, Pai Ching

; Caramelli, Bruno

; Marques, André Coelho

; Calderaro, Daniela

; Fornari, Luciana Savoy

; Pinho, Claudio

; Feitosa, Alina Coutinho Rodrigues

; Polanczyk, Carisi Anne

; Rochitte, Carlos Eduardo

; Jardim, Carlos

; Vieira, Carolina L. Z.

; Nakamura, Debora Y. M.

; Iezzi, Denise

; Schreen, Dirk

; Adam, Eduardo Leal

; D’Amico, Elbio Antonio

; Lima, Emerson Q. de

; Burdmann, Emmanuel de Almeida

; Mateo, Enrique Indalecio Pachón

; Marcondes-Braga, Fabiana Goulart

; Machado, Fabio S.

; Paula, Flavio J. de

; Carmo, Gabriel Assis Lopes do

; Feitosa-Filho, Gilson Soares

; Prado, Gustavo Faibischew

; Lopes, Heno Ferreira

; Fernandes, João R. C.

; Lima, José J. G. de

; Sacilotto, Luciana

; Drager, Luciano Ferreira

; Vacanti, Luciano Janussi

; Rohde, Luis Eduardo Paim

; Prada, Luis F. L.

; Gowdak, Luis Henrique Wolff

; Vieira, Marcelo Luiz Campos

; Monachini, Maristela Camargo

; Macatrão-Costa, Milena Frota

; Paixão, Milena Ribeiro

; Oliveira Junior, Mucio Tavares de

; Cury, Patricia

; Villaça, Paula R.

; Farsky, Pedro Silvio

; Siciliano, Rinaldo F.

; Heinisch, Roberto Henrique

; Souza, Rogerio

; Gualandro, Sandra F.M.

; Accorsi, Tarso Augusto Duenhas

; Mathias Júnior, Wilson

Arquivos Brasileiros de Cardiologia

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sep 2017, Volume 109 N. 3 Pages 1 - 104

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.5935/abc.20170140 100302 downloads

11.

Effects of changes in micro-weather conditions on structural features, total protein and carbohydrate content in leaves of the Atlantic rain forest tree golden trumpet (Tabebuia chrysotricha)
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Moura, B. B.

; Almeida, P. H.

; Balliana, A. G.

; Cobos, J. Y. G.

; Erbano, M.

; do Prado, K. A.

; Vudala, S. M.

; Wosch, L.

; Pereira-Netto, A. B.

Brazilian Journal of Biology

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jul 2017, Volume 77 N. 3 Pages 535 - 541

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Ipê amarelo é uma árvore nativa da floresta Atlântica brasileira, encontrada em uma ampla distribuição latitudinal. Neste estudo, nós investigamos os efeitos potenciais de alterações de curto prazo nas condições micro-climáticas sobre características estruturais, proteína e carboidratos histoquimicamente marcados, de folhas de ipê amarelo, usando estratégias de análise estrutural e histoquímicas. As folhas foram marcadas em quatro condições microclimáticas distintas: 1. Tarde, após um dia quente e ensolarado; 2. Amanhecer, após um dia quente e ensolarado; 3. Ao meio-dia, de um dia quente e ensolarado; e 4. Ao meio-dia, de um dia frio e nublado. Folíolos expostos à baixa irradiância luminosa apresentaram cloroplastos achatados, uniformemente distribuídos no interior das células, por todo o parênquima paliçádico, enquanto que folíolos expostos à alta irradiância apresentaram cloroplastos achatados e arredondados, nas células superiores e inferiores do parênquima paliçádico, respectivamente. A marcação mais intensa para proteína foi observada para folhas coletadas no momento mais frio de coleta, enquanto que a marcação mais fraca foi observada para folhas coletadas após um dia quente e ensolarado. Os grãos de amido maiores e mais numerosos foram observados em folhas coletadas durante a tarde de dia quente e ensolarado, enquanto que os menores e menos numerosos grãos de amido foram observados em folhas coletadas ao amanhecer.

Abstract Golden trumpet, Tabebuia chrysotricha, is a native tree from the Brazilian Atlantic rain forest, with a broad latitudinal distribution. In this study, we investigated the potential effects of short-term changes in micro-weather conditions on structural features, and total protein and carbohydrate content of golden trumpet leaves, using structural and histochemical approaches. Leaves were harvested in four different micro-weather conditions: 1. Afternoon, after a hot, sunny day; 2. at dawn, after a previously hot, sunny day; 3. at noon, of a hot, sunny day; and 4. at noon, of a cold, cloudy day. Leaflets exposed to low light irradiance showed flattened chloroplasts, uniformly distributed within the cells, throughout the palisade parenchyma. Conversely, leaflets exposed to high light irradiance presented flattened and rounded chloroplasts, in the upper and lower palisade parenchyma cells, respectively. The strongest protein staining was found for leaves harvested at the coldest period, whereas the weakest protein staining was found for leaves harvested after a hot, sunny day. The largest and most numerous starch grains were found for leaves harvested in the afternoon, after a hot, sunny day. Conversely, the smallest and less numerous starch grains were found for leaves harvested at dawn. Analysis of the data reported herein suggests that the leaflet responses to transient changes in micro-weather conditions are likely to contribute to the golden trumpet successful establishment in the broad latitudinal distribution in which the species is found.

https://doi.org/10.1590/1519-6984.18815 990 downloads

12.

Cinerascetins, New Peptides from Hypsiboas cinerascens: MALDI LIFT-TOF-MS/MS de novo Sequence and Imaging Analysis
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Almeida, Richardson A.

; Gordo, Marcelo

; Silva, Felipe M. A. da

; Araújo, Rafael C. de

; Ramada, Marcelo H. S.

; Abrão, Fernando Y.

; Costa, Túlio O. G.

; Koolen, Hector H. F.

; Souza, Afonso D. L. de

; Bloch Jr., Carlos

Journal of the Brazilian Chemical Society

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nov 2015, Volume 26 N. 11 Pages 2290 - 2297

Abstract: En | Text: En | PDF: En

The continuous search for antimicrobial candidates pushes the pursuit of compounds in the most diverse organisms. Amphibians are known as a prolific source of antibacterial peptides. Based on the rich biodiversity of the Amazon region the unexplored green-tree frog (Hypsiboas cinerascens) was studied for its skin secretion peptide content. Chromatographic separations and established tandem mass spectrometry (MS/MS) methods were used for sequencing the primary structures of the purified compounds. De novo sequencing lead to the identification of five new peptides related to hylaseptin P1, displaying an aminated C-terminal. Sequencing of the complementary deoxyribonucleic acid (cDNA) analysis allowed the disambiguation of isobaric amino-acids for C-01. Matrix assisted laser desorption ionization (MALDI) was carried out, demonstrating the in situ co-occurrence of the identified peptides in the dorsal skin. The major peptide C-01 was synthesized and assayed against a selection of microorganisms displaying minimal inhibitory concentrations (MICs) ranging from 4 to 16 µM.

https://doi.org/10.5935/0103-5053.20150219 1136 downloads

13.

O processo de construção da gestão regional da saúde no estado de São Paulo: subsídios para a análise
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Mendes, Áquilas

; Louvison, Marília C. P.

; Ianni, Aurea M. Z.

; Leite, Marcel Guedes

; Feuerwerker, Laura C. M.

; Tanaka, Oswaldo Y.

; Duarte, Ligia

; Weiller, José Alexandre Buso

; Lara, Natália Carolina Cairo

; Botelho, Lygia de Andrade Machado

; Almeida, Cristiane A. L.

Saúde e Sociedade

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jun 2015, Volume 24 N. 2 Pages 423 - 437

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Este artigo apresenta os resultados gerais da pesquisa sobre o processo de construção da gestão regional no estado de São Paulo, durante a discussão do COAP/redes de atenção, com o intuito de fornecer subsídios para a compreensão deste processo de pactuação regional da saúde, com enfoque nas regiões de saúde de Bauru, Baixada Santista, Grande ABC e Vale do Ribeira, no estado de São Paulo. Além dos resultados apresentados sobre as regiões de saúde estudadas, a metodologia utilizada no desenvolvimento da construção dos perfis das regiões constitui em si uma proposta metodológica de análise de perfis regionais de saúde. A primeira parte do artigo apresenta a metodologia geral adotada para a análise das regiões de saúde; a segunda, abrange os resultados e a discussão da pesquisa, organizados em dois itens. O primeiro destes itens refere-se à análise dos perfis das cinco regiões de saúde pesquisadas no Estado. O segundo, analisa os principais aspectos do processo de pactuação regional da saúde em São Paulo, destacando potencialidades e limites, a partir de entrevistas realizadas com gestores municipais e apoiadores do Conselho de Secretários Municipais de Saúde de São Paulo dessas regiões.

This article presents the main results of the survey on the regional management building process in the State of São Paulo, during the discussion of the COAP / Care Networks, in order to provide a basis for understanding this process of regional health pact, focusing on the metropolitan areas of Bauru, Santos, Grande ABC and the Ribeira Valley. In addition to the results presented on the health regions studied, the methodology used in the development of the construction of profiles of the regions is itself a proposal for a methodology of analyzing regional health profiles. The first section presents the general methodology for analyzing health regions. The second part covers the results and discussion of the research, organized into two items. The first refers to the analysis of the profiles of the five São Paulo health regions surveyed. The second item analyzes the main aspects of the process of regional health pact in São Paulo, highlighting strengths and limitations, based on interviews with municipal managers and supporters of the Council of Municipal Health Secretaries of São Paulo in these regions.

https://doi.org/10.1590/S0104-12902015000200003 228 downloads

14.

O processo de construção da gestão regional da saúde no estado de São Paulo: subsídios para a análise
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Mendes, Áquilas

; Louvison, Marília C. P.

; Ianni, Aurea M. Z.

; Leite, Marcel Guedes

; Feuerwerker, Laura C. M.

; Tanaka, Oswaldo Y.

; Duarte, Ligia

; Weiller, José Alexandre Buso

; Lara, Natália Carolina Cairo

; Botelho, Lygia de Andrade Machado

; Almeida, Cristiane A. L.

Saúde e Sociedade

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jun 2015, Volume 24 N. 2 Pages 423 - 437

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

https://doi.org/10.1590/S0104-12902015000200003 4645 downloads

15.

Diversity of ferns and lycophytes in Brazil
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Prado, Jefferson

; Sylvestre, Lana da S.

; Labiak, Paulo H.

; Windisch, Paulo G.

; Salino, Alexandre

; Barros, Iva C.L.

; Hirai, Regina Y.

; Almeida, Thaís E.

; Santiago, Augusto C.P.

; Kieling-Rubio, Maria A.

; Pereira, Anna Flora de N.

; Øllgaard, Benjamin

; Ramos, Carla G.V.

; Mickel, John T.

; Dittrich, Vinicius A.O.

; Mynssen, Claudine M.

; Schwartsburd, Pedro B.

; Condack, João Paulo S.

; Pereira, Jovani B.S.

; Matos, Fernando B.

Rodriguésia

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2015, Volume 66 N. 4 Pages 1073 - 1083

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Resumo Esta compilação de samambaias e licófitas do Brasil é uma atualização daquela de 2010, no Catálogo de Plantas e Fungos do Brasil. A metodologia consistiu na reunião de dados de listas regionais, revisões de grupos e bancos de dados selecionados. Especialistas convidados melhoraram a lista através do acesso a um sítio da web do Jardim Botânico do Rio Janeiro. Os resultados apontam uma diversidade de 1.253 espécies, sendo 1.111 samambaias e 142 licófitas. Este número é 6,5% maior que o anterior (1.176 espécies). As espécies endêmicas decresceram de 38,2% para 36,7%. Foram reconhecidas 36 famílias e 133 gêneros (vs. 33 famílias, 121 gêneros em 2010). As dez famílias mais diversas são: Pteridaceae (196 espécies), Dryopteridaceae (179), Polypodiaceae (164), Hymenophyllaceae (90), Thelypteridaceae (86), Aspleniaceae (78), Lycopodiaceae (64), Selaginellaceae (55), Anemiaceae (51) e Cyatheaceae (45). Os três gêneros mais diversos continuam sendo Elaphoglossum (87 espécies), Thelypteris (85) e Asplenium (74). O Domínio Fitogeográfico mais rico continua sendo a Mata Atlântica (883 espécies) e também com mais espécies endêmicas e ameaçadas, seguido pela Amazônia (503 espécies), Cerrado (269), Pantanal (30), Caatinga (26) e Pampa (oito). Minas Gerais permanece como o estado com maior riqueza (657 espécies vs. 580 em 2010).

Abstract This compilation of ferns and lycophytes in Brazil is an update of the one published in 2010 in Catálogo de Plantas e Fungos do Brasil. The methodology consisted in collecting data from regional checklists, taxonomic revisions, and selected databases. Invited specialists improved the list accessing a website housed at the Jardim Botânico do Rio de Janeiro. The results show 1,253 species: 1,111 of ferns and 142 of lycophytes. This number is 6.5% higher than the previous one (1,176 spp.). The percentage of endemic species decreased from 38.2% to 36.7%. We recognized 36 families and 133 genera (vs. 33 families, 121 genera in 2010). The 10 most diverse families are Pteridaceae (196 spp.), Dryopteridaceae (179), Polypodiaceae (164), Hymenophyllaceae (90), Thelypteridaceae (86), Aspleniaceae (78), Lycopodiaceae (64), Selaginellaceae (55), Anemiaceae (51), and Cyatheaceae (45). The three most diverse genera are still Elaphoglossum (87 spp.), Thelypteris (85), and Asplenium (74). The richest phytogeographic domain continues to be in the Atlantic Rainforest with 883 species which also has the largest number of endemic and threatened species, followed by the Amazon Rainforest (503), Cerrado (269), Pantanal (30), Caatinga (26), and Pampa (eight). Minas Gerais remains as the richest state (657 spp. vs. 580 in 2010).

https://doi.org/10.1590/2175-7860201566410 6608 downloads

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