ABSTRACT This study investigated the effectiveness of using the X-ray technique to assess the viability of papaya seeds. The X-ray images were performed in eight replicates of 25 seeds, placed on transparent acrylic plates on double-sided adhesive tape, previously identified, and then submitted to radiation in X-ray equipment "HP Faxitron". After exposure to X-ray radiation, the seeds were submitted to the germination test in rolls of germitest paper and kept in a germination chamber at 20-30 °C for 30 days. Seedlings were photographed and evaluated as normal seedlings, abnormal and non-germinated seeds. Afterward, the X-ray images and the seedlings from the same seeds were visually compared, and the X-ray images clearly showed seeds with and without internal filling. All abnormal seedlings were full seeds; therefore, X-ray images were not able to separate normal and abnormal. A 100% accuracy was verified by comparing the X-ray images and their respective seedlings, full seeds originated normal or abnormal seedlings, and empty seeds corresponded to non-germinated seeds. Therefore, using X-ray images is a promising technique to identify the viability of papaya seeds. The automated analysis of X-ray images is a simple, fast, and efficient technique to provide information on seed viability and generate parameters related to their germination capacity.

ABSTRACT Stress caused by defoliation can compromise the physiological quality of soybean seeds. However, there is evidence that the application of nitrogen (N) can be an alternative to reduce the qualitative losses in seeds caused by defoliating insects. The objective of this study was to evaluate the effectiveness of topdressing N fertilization in improving the physiological quality of soybean seeds from plants subjected to artificial defoliation. The experimental design was a randomized block arranged in a 2×4 factorial scheme with four replicates. The treatments consisted of two levels of defoliation (33 and 66%) and four doses of N (0, 50, 100, and 150 kg ha-1), using urea (45% N), applied two days after defoliation. Artificial defoliation was performed during the R3 stage by removing one (33%) and two leaflets (66%) from each trifoliate leaf under field conditions. At stage R5.1, leaf area was quantified, and after harvesting, germination, emergence, length, total dry mass, electrical conductivity, total protein content, mass of 1000 seeds, and tetrazolium (vigor and viability) tests were carried out. It was concluded that increasing the defoliation from 33 to 66% impaired the physiological quality of soybean seeds. The use of nitrogen fertilization for coverage after defoliation at the beginning of the reproductive phase cannot minimize qualitative losses in seeds.

ABSTRACT Harvest delay can alter the physiological and sanitary quality of sorghum seeds due to the longer exposure time of seeds to adverse environmental conditions in the field. In this study, we investigated the impact of delayed Sorghum sp. harvest on the physiological and sanitary quality of seeds in four hybrids grown in the tropical soil of the Brazilian Cerrado region. Plants from four Sorghum sp. hybrids (IPA SUDAN 4202, BRS 658, BRS 810, and BRS 373) were grown and submitted to four seed harvest periods (0, 7, 14, and 21 days after stage 9 - seed physiological maturity). The treatments were arranged in a completely randomized block design in a 4 × 4 factorial, with four replicates. At each harvest period, the thousand-seed weight, water content, first count of the germination test, germination, seedling emergence, emergence speed index, seedling dry matter, accelerated aging, electrical conductivity, and sanitary quality of seeds were determined. Seeds of higher physiological and sanitary quality of the IPA SUDAN 4202 and BRS 658 sorghum hybrids can be obtained when the harvest is carried out up to 7 days after the physiological maturity stage. However, the delay in seed harvesting at 14 and 21 days after physiological maturity results in a higher incidence of Colletotrichum sp., Curvularia sp., Rhizoctonia sp., and Alternaria sp. on the seeds of these hybrids. Delaying the harvest of sorghum seeds from 7 days after the physiological maturity stage compromises seed vigor and viability and increases the incidence of pathogens.

ABSTRACT Among the main problems that compromise the development of forest species are water and salt stresses, especially in the early stages of development, when seedlings are more sensitive. Thus, the objective of this study was to evaluate the morphophysiological and biochemical changes in Enterolobium contortisiliquum seedlings subjected to abiotic stresses. The experimental design was randomized complete blocks (RCB), composed of five treatments, with four replicates, and the experimental plot consisted of twenty plants. From the 31st day after sowing, when the seedlings reached approximately 15 cm in height, they began to receive the treatments, which consisted of different levels of electrical conductivity of irrigation water (0.3; 2; 4; 6; 8 dS m-1) for salt stress and periods of water restriction (0; 4; 8; 12 and 16 days without irrigation) for water stress. The traits evaluated were: plant height, root length, stem diameter, number of leaves, leaf area, total dry matter, Dickson quality index, height/shoot dry matter ratio, total soluble sugars, starch, total amino acids and proline. An increase in the electrical conductivity of irrigation water and days without irrigation compromised the development of E. contortisiliquum seedlings, which was intensified from 2 dS m-1 and four days without irrigation for salt and water stresses, respectively. The mechanism of survival of E. contortisiliquum to abiotic stresses involves reduction in the growth and quality of seedlings, with activation of biochemical defense mechanisms of the species.

ABSTRACT The objective was to verify if the primary root emission can be recommended as a vigor test for soybean seeds. For this, five lots of varieties ANTA 82, NA7337 and BRS1074IPRO were evaluated by first count, germination, emergence, emergence speed index and tetrazolium tests. In the root protrusion test at 20, 25 and 30 ºC, two evaluation criteria were adopted: primary root emission precocity index (PREPI) and percentage of seeds that emitted the primary root (PRE). The seeds counted were those with a primary root equal to or greater than 2 mm, every 12 h from the installation of the test until 120 h. The design was completely randomized and means were compared, within each evaluation time, using the Scott-Knott test at 5% probability. Pearson’s linear correlation was performed for PREPI and PRE with emergence. PREPI was not considered efficient. For the PRE, at 20 ºC, there was a correlation at 72 h, for the three varieties. At 25 ºC there was a correlation for a period of 36 h for ANTA 82 and NA7337 and 60 h for BRS1074IPRO. At 30 ºC there was a correlation in the time of 36 h for the three cultivars. Considering that speed in obtaining results is important in vigor tests, it is concluded that primary root emission for 36 h after installing the test at 30 ºC is recommended as a vigor test in the quality control of soybean seeds.

Abstract: Melon is one of the most consumed cucurbits and commercially significant cucurbits in the world. It is planted predominantly through seedlings, and seed quality is essential in this process. Among the rapid tests used in seed analysis, the pH testing of exudate has shown promise for several cultivated species. Thus, this study aimed to verify the viability of melon (Cucumis melo L.) seeds through the pH testing of exudate. For this, seeds of the hybrids Premier and Supreme (three lots each) were subjected to germination and vigor tests (first germination count, tetrazolium, accelerated aging, emergence speed index, and emergence). The pH testing of exudate was evaluated using phenolphthalein and sodium carbonate solutions. The results indicated the possibility of classifying the lots into three quality levels for both hybrids, similar to most tests used to assess initial quality. Therefore, the pH testing of exudate was effective in determining the viability of lots of melon seeds and can be conducted with a soaking period of 120 minutes at 25 or 30 °C.

Resumo: O meloeiro é uma das cucurbitáceas mais consumidas e de maior interesse comercial do mundo. Seu plantio é realizado predominantemente por meio de mudas, sendo a qualidade das sementes fundamental neste processo. Dentre os testes rápidos utilizados na análise de sementes, o pH do exsudato tem se mostrado promissor para várias espécies cultivadas. Com isso, objetivou-se verificar a viabilidade de sementes de melão (Cucumis melo L.) por meio do teste de pH do exsudato. Para isso, sementes dos híbridos Premier e Supreme (três lotes de cada) foram submetidas aos testes de germinação e vigor (primeira contagem de germinação, tetrazólio, envelhecimento acelerado, índice de velocidade de emergência e emergência). O teste de pH de exsudato foi avaliado com a utilização de soluções de fenolftaleína e carbonato de sódio. Os resultados indicaram que para ambos os híbridos foi possível classificar os lotes em três níveis de qualidade, semelhantes a maioria dos testes utilizados na avaliação da qualidade inicial. Com isso, o teste de pH do exsudato mostrou-se eficaz para determinar a viabilidade de lotes de sementes de melão, podendo ser conduzido com período de embebição de 120 minutos a 25 ou 30 °C.

Resumo: A tecnologia de aplicação do plasma atmosférico consiste em um método rápido e prático, que apresenta potencial de modificação da superfície tegumentar de sementes com dureza tegumentar. O objetivo desta pesquisa foi avaliar o efeito da aplicação do plasma frio atmosférico em sementes de Pityrocarpa moniliformis, verificando suas implicações sobre a impermeabilidade do tegumento, germinação e vigor. As sementes foram submetidas ao plasma por 1,5; 2,0; 3,0; 4,0 e 5,0 minutos, utilizando-se como controle, sementes sem nenhum tratamento. Após a aplicação, determinou-se a molhabilidade do tegumento, curva de embebição e condutividade elétrica da solução de embebição. As sementes também foram submetidas ao teste de germinação, tendo-se, a partir dos seus resultados determinado a viabilidade, mediana, uniformidade e assimetria da germinação. As sementes submetidas ao plasma por 4,0 e 5,0 minutos apresentaram os menores ângulos de contato aparente, 64 e 61°, respectivamente, caracterizando maior molhabilidade do tegumento, entre os tratamentos testados. Maior germinação foi observada nos tratamentos com aplicação do plasma quando comparado com o controle. A tecnologia de aplicação do plasma atmosférico tem potencial para ser utilizada como acelerador da germinação de sementes de P. moniliformis.

Abstract: Atmospheric plasma application technology consists of a fast and practical method, which has the potential to modify the surface of seeds with integumentary hardness. The aim of this research was to evaluate the effect of applying atmospheric cold plasma on Pityrocarpa moniliformis seeds, assessing its implications on the seed coat impermeability, germination and vigor. Cells were subjected to plasma for 1.5, 2.0, 3.0, 4.0 and 5.0 minutes, using seeds without any treatment as a control. After application, the seed coat wettability, imbibition curve and electrical conductivity of the soaking solution were determined. Seeds were also subjected to the germination test, and its results were used to determine viability, median, uniformity and asymmetry of germination. Seeds subjected to plasma for 5.0 and 4.0 minutes showed the lowest apparent contact angles, 64 and 61°, respectively, characterizing greater wettability of the seed coat among the treatments tested. Higher germination was observed in the treatments with plasma application when compared to the control. Atmospheric plasma application technology has the potential to be used as an accelerator of P. moniliformis seed germination.

Resumo: Recentemente está crescendo a necessidade de tecnologias não invasivas, rápidas e precisas que possam estimar a qualidade das sementes. Entre essas tecnologias, destaca-se a análise de imagens de raios X para avaliação da morfologia interna das sementes. Assim, o objetivo deste estudo foi avaliar a eficiência do software SARS na análise de radiografias digitais de sementes de braquiária, denominado SARS (Sistema de Análise de Radiografias de Sementes) na avaliação da qualidade física das sementes. Cinco lotes comerciais de sementes de Urochloa decumbens cv. Basilisk foram utilizados. As sementes foram produzidas na safra de 2018/2019. As imagens radiográficas das sementes foram analisadas por meio do SARS, por meio das quais foram obtidas características físicas. As sementes foram submetidas ao teste de germinação, no qual foram mensuradas variáveis relacionadas à qualidade fisiológica. Foi possível observar que as sementes com maior potencial de germinação e vigor apresentaram correlações fortes e significativas com algumas das variáveis físicas obtidas pelo SARS. Assim, a alta correlação do comprimento de plântulas e a densidade relativa das sementes é importante para validar o método de análise de imagens radiográficas de sementes. O SARS mostrou-se uma ferramenta eficiente para análise de radiografias digitais de sementes de braquiária, gerando descritores que dão suporte a análise morfométrica e interna das sementes, que podem ser associados à sua qualidade fisiológica. Os parâmetros físicos obtidos pela técnica têm estreita relação com a germinação e o vigor das sementes.

Abstract: Recently is growing the need for non-invasive, fast, and accurate technologies that can predict seed quality. Between these technologies, X-ray image analysis stand out for evaluation of the internal morphology of the seeds. Thus, the aim of the present study was to evaluate the efficiency of a specialized software for analyzing digital radiographs of Urochloa decumbens seeds called SARS (Sistema de Análise de Radiografias de Sementes - Seed Radiograph Analysis System). Five comercial seed lots of U. decumbens cv. Basilisk were used. The seed lots were produced in the 2018/2019 crop season. Radiographic images of the seeds were analyzed in SARS, through which physical characteristics were obtained. The seeds were then subjected to germination test, in which variables related to the physiological quality were evaluated. It was possible to observe that the seeds with greater germination and vigor showed strong and significant correlations with some of the physical variables obtained using SARS. Thus, high correlation of seedling length and relative seed density is important for validating the seed radiographic image analysis method. SARS proved to be an efficient tool for analyzing digital radiographs of U. decumbens seeds. It can generate descriptors which support morphometric and internal analysis of the seeds. Physical parameters obtained by using the technique have close relationship with the germination and vigor of the seeds.

RESUMO O significativo potencial econômico de Swietenia macrophylla tem causado redução intensa nas populações nativas, colocando em risco sua sobrevivência, exigindo assim, medidas para promover adequada avaliação do estado fisiológico das sementes, visando estabelecimento de um programa de qualidade de produção de mudas. Assim, o objetivo do presente estudo foi avaliar o efeito da temperatura e da luz sobre a germinação de sementes e o crescimento de plântulas de Swietenia macrophylla. O delineamento experimental foi o inteiramente casualizado, com dez tratamentos de quatro réplicas com 50 sementes, e constou de: temperaturas constantes de 23, 29, 35 e 41 °C, com fotoperíodo de 24 e 12 h de luz, e alternadas de 25-35 oC e 30-40 oC, com 12 h de luz. Avaliou-se o teor de água, germinação, índice de velocidade de germinação, diâmetro do coleto, comprimento de raiz e parte aérea, massa seca de raiz e parte aérea de plântulas. Germinação na temperatura de 41 oC é letal para sementes de S. macrophylla. O teste de germinação com temperatura constante de 29 oC sob 24 h de luz favorece o processo germinativo das sementes e o crescimento inicial das plântulas.

ABSTRACT The significant economic potential of Swietenia macrophylla has caused immense reductions in the native populations, jeopardizing their survival. Restoring these populations requires the establishment of quality seedling production, which depends on adequate evaluation of the physiological state of seeds. Thus, the aim of this study was to evaluate the effect of temperature and light on seed germination and seedling growth of S. macrophylla. The experimental design was completely randomized, with ten treatments of four replicates of 50 seeds. Treatments consisted of: constant temperatures of 23, 29, 35 and 41 °C, with photoperiods of 24 and 12 h of light, and alternating temperatures of 25-35 °C and 30-40 °C, with 12 h of light. Water content, germination, germination speed index, stem diameter, root length, shoot length, root dry mass and shoot dry mass of seedlings were evaluated. At 41 oC, S. macrophylla seeds failed to germinate. A constant temperature of 29 oC under 24 h of light favors the germination process of seeds and initial seedling growth.

Rice seeds coating with microparticulate zinc oxide can maximize the absorption of this micronutrient by the seedling and favor seeds vigor when compared with zinc sulfate. This work aimed to evaluate the sources and doses of zinc in seed quality and in the nutrition of rice seedlings. The experiment consisted of a completely randomized design with four replications, five doses of zinc in the form of oxide (0; 20; 40, 80 and 160 g kg-1), and a control treatment of zinc in the form of sulfate (40 g kg-1). The following parameters were evaluated: germination, accelerated aging, seedlings emergence, emergence speed index, cold test, dry matter, and zinc concentration in the root and shoot of the seedlings. Seed coating with zinc, regardless of the source, improved seed physiological quality and seedling nutrition. Zinc sources did not affect germination; however, the oxide form induced better seed vigor, except for the highest dose (160 g kg-1). Increasing doses of zinc oxide augmented the concentration of this nutrient in rice seedlings dry matter. Zinc coating of rice seeds in the form of microparticulate, depending on the dose, can maximize seed vigor and increase the concentration of this micronutrient in the seedlings when compared with zinc sulfate, the traditional source.

El revestimiento de semillas de arroz con óxido de zinc microparticulado puede maximizar la absorción de este micronutriente por las plántulas y favorecer el vigor de las semillas en comparación con el sulfato de zinc. Este trabajo tuvo como objetivo evaluar las fuentes y dosis de zinc en la calidad de semillas y en la nutrición de plántulas de arroz. El experimento fue desarrollado en delineamiento completamente al azar con cuatro repeticiones, con un testigo sin aplicación de zinc, cuatro dosis de óxido de zinc (20, 40, 80 y 160 g kg-1 de Zn), y un tratamiento en la forma de sulfato de zinc, 40 g kg-1 de Zn. Se evaluaron los siguientes parámetros: germinación, velocidad de envejecimiento, emergencia de plántulas, índice de velocidad de emergencia, prueba fría de semillas, materia seca y acumulación de zinc en la raíz y parte aérea de las plántulas. La cobertura de semillas con zinc, independientemente de la fuente, mejoró la calidad fisiológica de las semillas y la nutrición de las plántulas de arroz. Las fuentes de zinc no alteraron la germinación; sin embargo, el óxido de zinc proporcionó un mejor vigor de las semillas, con la excepción de la mayor dosis, 160 g kg-1. El aumento en la dosis de óxido de zinc aumentó la acumulación de ese nutriente en la materia seca de las plántulas de arroz. El recubrimiento con zinc en semillas de arroz en forma de micropartículas, dependiendo de la dosis, puede maximizar el vigor de las semillas y aumentar la concentración de este micronutriente en las plántulas en comparación con el sulfato de zinc, que es la fuente tradicional utilizada por los productores.

RESUMO O trabalho objetivou avaliar a eficiência do teste de pH do exsudato na qualidade fisiológica de sementes de arroz, com e sem tegumento. Para o teste, o delineamento experimental foi inteiramente casualizado, com sete lotes de arroz e quatro repetições. Os lotes foram submetidos a determinação do teor de água e avaliação da qualidade fisiológica inicial por meio dos testes de germinação, primeira contagem de germinação, emergência, índice de velocidade de emergência e tetrazólio. O teste de pH do exsudato foi conduzido com 100 sementes de cada tratamento, com tegumento e sem tegumento. As sementes foram embebidas individualmente em 2,0 mL de água destilada, por diferentes períodos (20, 40 e 60 minutos) e três temperaturas (20, 25 e 30 °C). Os resultados foram submetidos ao teste de Scott Knott a 5% de probabilidade. O teste do pH do exsudato é promissor para avaliar a qualidade fisiológica de sementes de arroz, detectando diferenças de vigor entre lotes comercializáveis. Este teste deve ser conduzido com sementes de arroz sem tegumento, na temperatura de 25 °C, com tempo de embebição de 60 minutos.

ABSTRACT The objective of this study was to evaluate the efficiency of a pH test of the exudate (with and without tegument) as an indicator of the physiological quality of rice seeds. The experimental design was completely randomized, with seven lots and four replications. The seven lots were assessed for water content and initial physiological quality by means of germination, first germination count, emergence, emergence speed index and tetrazolium tests. The pH test of the exudate was conducted with 100 seeds of each treatment, with tegument and without tegument. The seeds were individually soaked in 2.0 mL distilled water for different periods (20, 40, and 60 minutes) and three temperatures (20, 25, and 30 °C). The results were submitted to the Scott Knott test at 5% probability. The pH test of the exudate is a promising method to evaluate the physiological quality of rice seeds by detecting differences in vigor between marketable lots. This test should be conducted with rice seed without tegument, at 25 °C, with a soak time of 60 minutes.

RESUMO O uso de dessecantes pode permitir a obtenção de sementes com máxima qualidade fisiológica e sanitária, devido o menor período em que as sementes ficam expostas as condições adversas no campo aguardando o estádio de maturação para colheita. O objetivo neste trabalho foi avaliar o efeito de dessecantes químicos e épocas de colheita sobre a qualidade fisiológica e sanitária em sementes de soja. O ensaio foi realizado em delineamento em blocos casualizados, disposto em esquema fatorial (4 × 3) + 1, sendo quatro herbicidas para a dessecação (gramoxone - 2 L ha-1, glufosinato - 2 L ha-1, reglone - 1,5 L ha-1, saflufenacil 40 g ha-1) aplicado no estádio fenológico R7.1 (maturidade fisiológica e 65% de umidade), com três épocas de colheita (0; sete e 14 dias após o estádio fenológico R8 - maturação plena), mais o controle (ausência de dessecante e colhida em R8), com quatro repetições. O uso do dessecante gramoxone e as sementes colhidas sete dias após R8 proporciona maior qualidade fisiológica de sementes de soja, conforme observado nas variáveis germinação, danos por umidade, danos mecânicos e a incidência de Colletotrichum sp., Phomopsis sp., Penicilium sp., Fusarium sp., Cercospora kikuchii e Alternaria sp.. A colheita de sementes no estádio R8+14 promove as maiores perdas na qualidade das sementes. O uso de glufosinato de amônio e saflufenacil como dessecantes não são recomendados, devido à redução na qualidade fisiológica das sementes de soja.

ABSTRACT The use of desiccants may result in seeds with high physiological and sanitary quality due to the shorter period of exposure to field adverse conditions before the maturity stage for harvest. This study evaluated the effect of chemical desiccants and harvest times on the physiological and sanitary quality of soybean seeds. The experiment consisted of a randomized block design, arranged in a factorial scheme (4 × 3) + 1, with four replications. The first factor corresponded to four desiccation (grammoxone-2 L ha-1, glufosinate-2 L ha-1, reglone-1.5 L ha-1, and saflufenacil-40 g ha-1) applied at the R7.1 phenological stage (physiological maturity and 65% moisture with three harvest times (0, 7, and 14 days after the R8 phenological stage) and additional treatment (control, application of water only, and harvest at the R8 stage), with four replications. The use of the grammoxone desiccant and seeds harvested at seven days after the R8 stage resulted in soybean seeds of the highest physiological quality, as observed for the variables germination, moisture damage, mechanical damage, and incidences of Colletotrichum sp., Phomopsis sp., Penicillium sp. Fusarium sp., Cercospora kikuchii, and Alternaria sp. Seeds harvested at the R8 + 14 stage had the greatest losses in seed quality. The use of ammonium glufosinate and saflufenacil as desiccants is not recommended due to the reduction in the physiological quality of soybean seeds.

RESUMO A aplicação de silício está sendo difundida na agricultura como alternativa a ser integrada no manejo das culturas, por conta dos diversos benefícios relatados nas gramíneas acumuladoras e na resistência à estresses bióticos e abióticos. Assim, objetivou se avaliar a aplicação de silício via recobrimento de sementes e foliar nos componentes de produção do milho segunda safrinha. O delineamento experimental utilizado foi em parcelas subdivididas, na qual as parcelas foram compostas por doses de filossilicato (0; 5; 10; 15 e 20 g kg-1 de sementes) no recobrimento de sementes, e as subparcelas constituídas pela aplicação foliar de silicato de potássio (1,0 L ha-1 em pré-pendão e 15 dias após). As avaliações realizadas foram: altura de plantas, altura de inserção de espiga, diâmetro de espiga e sabugo, número de fileiras por espiga, número de grãos por fileira, massa de 100 grãos e produtividade. Não houve efeito da aplicação foliar de silicato de potássio na produtividade e componentes de produção do milho safrinha. O uso de filossilicato via recobrimento de sementes incrementou os componentes de produção e a produtividade do milho safrinha segunda safra.

ABSTRACT The application of silicon is becoming popular in agriculture as an alternative for integrated crop management, owing to the diverse benefits resulting from the accumulation of silicon in grasses and the resultant resistance conferred in plants against biotic and abiotic stresses. The objective of this study was to evaluate whether the application of silicon, via seed coating and leaf application, influences the production and yield components of maize in the second crop. The experimental design was divided into split plots, in which the plots were composed of phyllosilicate doses (0, 5, 10, 15, and 20 g kg-1 of seeds) in the seed coatings and the subplots were composed of foliar applications of potassium silicate (1.0 L ha-1 in pre-dip and 15 days after). We evaluated the plant height, spike insertion height, spike and corncob diameter, number of rows per spike, number of grains per row, the mass of 100 grains, and productivity. The foliar application of potassium silicate had no effect on the yield and yield components of the corn crop. The use of phyllosilicate via seed coating increased the production components and yield of the second corn crop.

RESUMO Este trabalho teve como objetivo avaliar a temperatura, o substrato e a luminosidade na germinação de sementes de chia, a fim de determinar procedimentos adequados para um teste de germinação. O experimento consistiu em um delineamento inteiramente casualizado, em esquema fatorial 4 x 5 x 2 (quatro substratos x cinco temperaturas x duas condições de luminosidade), com quatro repetições. As seguintes variáveis foram analisadas: primeira contagem de germinação; germinação; índice de velocidade de germinação e tempo médio de germinação. A germinação apresentou as maiores porcentagem de germinação a 15, 20, 25 e 30 °C para os substratos em papel, rolo de papel e areia; no entanto, na presença de luminosidade, o substrato em papel, a 25 °C, diferiu de todos os demais, resultando na maior média. Para todos os substratos, a menor germinação foi obtida a 35 °C, comprovando que o uso de altas temperaturas reduz a germinação das sementes de chia. O índice de velocidade de germinação apresentou os maiores valores a 25 e 30 °C para substratos em papel, rolo de papel e areia, tanto na presença quanto na ausência de luminosidade. Sementes de chia são consideradas como fotoblasticas neutras. O teste de germinação deve ser realizado com o substrato em papel, a 25 °C, com duração de sete dias, sendo a primeira contagem realizada aos quatro dias após a instalação do teste.

ABSTRACT The aim of the present study was to evaluate the effects of temperature, substrate, and luminosity on chia seeds in order to determine adequate conditions for a germination test. The experiment consisted of a completely randomized design, in a 4 × 5 × 2 factorial scheme (four substrates × five temperatures × two luminosity conditions), with four replications. The following variables were analyzed: first germination count, germination, germination speed index, and mean germination time. Germination was best at 15, 20, 25, and 30 °C for the on-paper, paper roll, and sand substrates. However, in the presence of luminosity and at 25 °C, the on-paper substrate had the highest mean. For all substrates, the lowest germination was achieved at 35 °C, proving that high temperatures reduce chia seed germination. Germination speed index was highest at 25 and 30 °C for on-paper, paper roll, and sand substrates, in both the presence and absence of luminosity. Chia seeds are considered to be neutral photoblasts. Germination tests should be performed with the on-paper substrate, at 25 °C, and with a duration of seven days. The first count should be carried out four days after establishment of the test.

RESUMO O teste de protrusão radicular é um teste de vigor, baseando no princípio de que sementes com alto vigor emitem a raiz primária mais rápido em comparação com as menos vigorosas. A chia é uma planta propagada por sementes, sendo motivo de pesquisa pelos seus benefícios a saúde, mas com poucas informações técnicas sobre a cultura. O objetivo do trabalho foi adequar à metodologia do teste de protrusão radicular para determinação do vigor de lotes de sementes de chia. Utilizou-se cinco lotes de sementes de chia, submetidos à avaliação da qualidade inicial por meio dos testes de germinação e vigor (primeira contagem de germinação, emergência e índice de velocidade de emergência). O teste de protrusão radicular foi realizado nas temperaturas de 20, 25 e 30 ºC, e avaliado a cada duas horas até 42 horas, onde se obteve a estabilidade da protrusão, e sendo utilizado o critério de contagem de 2 mm de raiz primária. O teste de protrusão radicular na temperatura de 20 ºC não distinguiu os lotes semelhantes à emergência. A 25 °C, foi possível classificar os lotes de forma semelhante à emergência após 30 horas. A temperatura de 30 °C foi eficiente na aceleração da protrusão radicular e separação dos lotes de sementes. O teste de protrusão radicular é sensível para detectar diferenças de vigor entre os lotes de sementes de chia, devendo ser conduzido por 30 horas a 25 ºC.

ABSTRACT The root protrusion test is a vigor test based on the principle that seeds with high vigor emit the primary root faster than less vigorous ones. Chia is a plant propagated by seeds and it is studied owing to its health benefits, but there is little technical information known about its culture. The objective of this work was to adapt the methodology of the root protrusion test to determine the vigor of chia seed lots. Five chia seed lots were submitted to an initial quality evaluation using germination and vigor tests (first germination, emergence, and emergence speed index). The root protrusion test was performed at temperatures of 20, 25 and 30 °C and evaluated every 2 h up to 42 h. During the test, protrusion stability was assessed and the criterion of 2 mm primary root count was used. The root protrusion test at 20 °C did not distinguish the lots in the same way as emergence. At 25 °C it was possible to classify lots after 30 h similarly to emergence. At 30 °C, root protrusion and the separation of seed lots was accelerated. Thus, the root protrusion test conducted for 30 h at 25 °C has sufficient sensitivity to detect differences in vigor between chia seed lots.