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Aim This study investigated the influence in vitro of different sodium hypochlorite (NaOCl) agitation protocols associated or not with DualRinse (HEDP) on the temperature of the solution. Methods Forty-eight premolars were instrumented and their apical third sealed to allow a closed irrigation system. The teeth remained immersed in a basin of warm water (37°C). The teeth were divided into the groups: G1 ( NaOCl+Passive Ultrasonic Irrigation (PUI)), G2 ( N a O C l / H E D P + P U I), G3 ( NaOCl + EasyClean (EC)) and G4 ( N a O C l / H E D P + E C). The canals were filled with the respective solutions and after 180 seconds the first temperature measurement was taken (T0). Then, the solutions were agitated, following the different protocols, for 60 seconds and a new measurement was performed (T60). The temperature was measured using a digital thermometer for type “K” sensors that was inserted into the middle third of the teeth. At the end of the measurements, the teeth were sectioned and prepared for scanning electron microscopy. The dentinal wall of middle third was graded according to the amount of debris and smear layer remaining on the walls. The results were analyzed using ANOVA test and Tukey’s multiple comparisons (p<0.05). Results G1 and G2 had an average increase in temperature of 1.1°C and 1.65°C, respectively (p>0.05). EasyClean caused a decrease in the temperature of the solutions in both groups, without a significant statistical difference with T0 (p>0.05). Regarding cleaning, it was only possible to observe clean dentinal tubules in the groups with the chelator. PUI discretely increased the temperature of the solution, regardless of the solution. The opposite effect was observed after activation with EasyClean. Conclusion The association of NaOCl with a chelating agent promoted the cleaning of the dentinal tubules.

https://doi.org/10.20396/bjos.v22i00.8668692

Flowering capacity and botanical seed production of sweet potato genotypes
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Brito, Orlando G

; Andrade Júnior, Valter C

; Lopes, Thabata Karoline

; Silva, Jeferson Carlos de O

; Firme, Tiago D

; Silva, Eduardo A da

; Azevedo, Sebastião Márcio de

Horticultura Brasileira

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dec 2021, Volume 39 N. 4 Pages 369 - 375

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A identificação de genótipos com maior capacidade de florescimento e produção de sementes é determinante para maior eficiência do melhoramento genético da cultura da batata-doce. Assim, o objetivo deste trabalho foi avaliar a capacidade de florescimento e a produção de sementes botânicas em genótipos de batata-doce. O trabalho foi conduzido no munícipio de Lavras, localizado na região sul de Minas Gerais, Brasil. Foram avaliados 22 genótipos de batata-doce pertencentes ao banco de germosplasma da Universidade Federal de Lavras (UFLA). Foram avaliados o período de florescimento (dias), número de sementes viáveis, peso total de sementes viáveis, peso de 1000 sementes e porcentagem de germinação. Os dados obtidos foram analisados por meio de estatística descritiva, estudo de correlações e análise de componentes principais. Os genótipos de batata-doce avaliados apresentaram elevada capacidade de florescimento e de produção de sementes botânicas. Verificou-se que a floração geralmente inicia-se 125 dias após o plantio e estende-se em média por 72 dias. Considerando futuras recombinações para promoção da capacidade de florescimento, produção de sementes e maior germinação, devem ser priorizados os genótipos BD-05, BD-26 e BD-44.

ABSTRACT The identification of genotypes with greater capacity for flowering and seed production is crucial for greater efficiency in the genetic improvement of the sweet potato crop. Thus, the objective of this work was to evaluate the flowering capacity and the production of botanical seeds in sweet potato genotypes. The work was carried out in the municipality of Lavras, located in the southern region of Minas Gerais, Brazil. Twenty-two sweet potato genotypes belonging to the germplasm bank of the Federal University of Lavras (UFLA) were evaluated. The characteristics evaluated were the flowering period (days), the number of viable seeds, the total weight of viable seeds, the weight of 1000 seeds and the percentage of germination. The obtained data were analyzed by means of descriptive statistics, study of correlations and analysis of main components. The sweet potato genotypes evaluated showed a high capacity for flowering and production of botanical seeds. Flowering usually starts 125 days after planting and extends on average for 72 days. Considering future recombination to promote flowering capacity, seed production and greater germination, genotypes BD-05, BD-26 and BD-44 should be prioritized.

https://doi.org/10.1590/s0102-0536-20210404 1 downloads

Curcuma longa hydrolate improves Nile tilapia survival in a recirculation rearing system, maintaining the animal homeostasis and modulating the gut microbial community
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PEREIRA, MARINA O

; HESS, JULIA D

; RODHERMEL, JULIO CESAR B

; FARIAS, DANIEL R

; SCHLEDER, DELANO D.

; ALVES, LUCIANO

; BERTOLDI, FABIANO C

; CHABAN, AMANDA

; ANDRADE, JAQUELINE I. A. DE

; JATOBÁ, ADOLFO

Anais da Academia Brasileira de Ciências

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2021, Volume 93 elocation e20210088

Abstract: En | Text: En | PDF: En

Abstract This study aimed to evaluate the effects of the dietary supplementation Curcuma longa hydrolate on Nile tilapia (Oreochromis niloticus) reared in a recirculation system. Hemato-immunological parameters, growth performance, nitrogen and phosphorus retention, as well as body composition and its interaction with the intestinal microbiota, were studied. Nile tilapia fingerlings (120) were distributed randomly in 8 polyethylene tanks (40 L). The experimental units were divided into two treatments, in quadruplicate: commercial diet supplemented with 2.5% of C. longa hydrolate and commercial diet without supplementation (control). After 45 days, the treatment supplemented hydrolate showed higher survival than the control group, 95.25% and 82.22%, receptively. In the blood profile, fish supplemented with hydrolate had a higher count of total leukocytes and neutrophils, as well as mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration, than control group. The hydrolate group showed a substantial increase in the relative abundance of Cetobacterium and Romboutsia, as well as lower diversity in gut microbiota. The dietary addition of C. longa hydrolate for Nile tilapia seems to have a beneficial effect on gut microbiota, in addition to a likely positive effect on the physiological performance of Nile tilapia by maintaining intestinal homeostasis and promoting survival in reared conditions.

https://doi.org/10.1590/0001-3765202120210088 34 downloads

Justificativa e delineamento do estudo “Tocilizumabe em pacientes com COVID-19 moderado a grave: estudo aberto, multicêntrico, randomizado, controlado” (TOCIBRAS)
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Farias, Danielle Leão Cordeiro de

; Prats, João

; Cavalcanti, Alexandre Biasi

; Rosa, Regis Goulart

; Machado, Flávia Ribeiro

; Berwanger, Otávio

; Azevedo, Luciano César Pontes de

; Lopes, Renato Delascio

; Avezum, Álvaro

; Kawano-Dourado, Leticia

; Damiani, Lucas Petri

; Rojas, Salomón Soriano Ordinola

; Oliveira, Cleyton Zanardo de

; Andrade, Luis Eduardo Coelho

; Sandes, Alex Freire

; Pintão, Maria Carolina

; Castro Júnior, Claudio Galvão de

; Scheinberg, Phillip

; Veiga, Viviane Cordeiro

Revista Brasileira de Terapia Intensiva

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sep 2020, Volume 32 N. 3 Pages 337 - 347

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Introdução: Os marcadores pró-inflamatórios desempenham papel importante na severidade de pacientes com COVID-19. Assim, terapêuticas anti-inflamatórias são agentes interessantes para potencialmente combater a cascata inflamatória descontrolada em tais pacientes. Delineamos um ensaio para testar tocilizumabe em comparação com o tratamento padrão, tendo como objetivo melhorar os desfechos por meio da inibição da interleucina 6, um importante mediador inflamatório na COVID-19. Métodos e análises: Este será um estudo aberto multicêntrico, randomizado e controlado, que comparará os desfechos de pacientes tratados com tocilizumabe mais tratamento padrão com o tratamento padrão isoladamente em pacientes com COVID-19 moderada a grave. Como critérios de inclusão, serão exigidos dois dos quatro critérios a seguir: dosagens de dímero D acima de 1.000ng/mL, proteína C-reativa acima de 5mg/dL, ferritina acima de 300mg/dL e desidrogenase lática acima do limite superior do normal. O objetivo primário será comparar a condição clínica no dia 15, conforme avaliação por meio de escala ordinal de 7 pontos aplicada nos estudos de COVID-19 em todo o mundo. O desfecho primário será avaliado por regressão logística ordinal assumindo razões de propensão proporcionais ajustadas pelas variáveis de estratificação (idade e sexo). Ética e disseminação: O TOCIBRAS foi aprovado pelos comitês de ética locais e central (nacional) do Brasil em conformidade com as atuais diretrizes e orientações nacionais e internacionais. Cada centro participante obteve aprovação do estudo por parte de seu comitê de ética em pesquisa, antes de iniciar as inscrições no protocolo. Os dados derivados deste ensaio serão publicados independentemente de seus resultados. Se tiver sua efetividade comprovada, esta estratégia terapêutica poderá aliviar as consequências da resposta inflamatória na COVID-19 e melhorar os resultados clínicos.

ABSTRACT Introduction: Pro-inflammatory markers play a significant role in the disease severity of patients with COVID-19. Thus, anti-inflammatory therapies are attractive agents for potentially combating the uncontrolled inflammatory cascade in these patients. We designed a trial testing tocilizumab versus standard of care intending to improve the outcomes by inhibiting interleukin-6, an important inflammatory mediator in COVID-19. Methods and analysis: This open-label multicentre randomized controlled trial will compare clinical outcomes of tocilizumab plus standard of care versus standard of care alone in patients with moderate to severe COVID-19. Two of the following four criteria are required for protocol enrolment: D-dimer > 1,000ng/mL; C reactive protein > 5mg/dL, ferritin > 300mg/dL, and lactate dehydrogenase > upper limit of normal. The primary objective will be to compare the clinical status on day 15, as measured by a 7-point ordinal scale applied in COVID-19 trials worldwide. The primary endpoint will be assessed by an ordinal logistic regression assuming proportional odds ratios adjusted for stratification variables (age and sex). Ethics and dissemination: The TOCIBRAS protocol was approved by local and central (national) ethical committees in Brazil following current national and international guidelines/directives. Each participating center had the study protocol approved by their institutional review boards before initiating protocol enrolment. The data derived from this trial will be published regardless of the results. If proven active, this strategy could alleviate the consequences of the inflammatory response in COVID-19 patients and improve their clinical outcomes.

https://doi.org/10.5935/0103-507x.20200060 1910 downloads

Associations between morphological and agronomic characteristics in garlic crop
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Andrade Júnior, Valter C de

; Guimarães, Amanda G

; Firme, Tiago D

; Costa, Aline Aparecida A

; Costa, Márcia Regina da

; Lopes, Thabata Karoline

; Souza, Rovilson José de

; Resende, Francisco V

Horticultura Brasileira

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jun 2019, Volume 37 N. 2 Pages 204 - 209

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO Correlações e análise de trilha entre características podem auxiliar na seleção de importantes atributos em programas de melhoramento genético. Assim, o objetivo deste trabalho foi avaliar correlações genotípicas, fenotípicas e realizar análise de trilha sob a produtividade comercial de bulbos de alho em função de variáveis morfológicas e agronômicas. O experimento foi realizado em 2017 na Universidade Federal dos Vales do Jequitinhonha e Mucuri e Universidade Federal de Lavras utilizando dez genótipos de alho, sendo oito experimentais e duas cultivares comerciais, Caçador e Jonas. Foram avaliadas: altura de plantas; número de folhas, número de bulbo total; massa de bulbo total, produtividade total de bulbo, produtividade comercial de bulbo e número de bulbilhos por bulbo total. As correlações genotípicas e fenotípicas foram positivas e significativas entre a produtividade comercial de bulbos com massa de bulbo, produtividade total e número de bulbilhos por bulbo total, indicando que a seleção baseada nessas características ajudará a aumentar o rendimento do alho. Entretanto, apenas o número de bulbilhos por bulbo apresentou efeito direto e efeito indireto relevante sobre a produtividade comercial de alho.

ABSTRACT Correlations and path analysis between characteristics can aid the selection of important attributes in breeding programs. Thus, the objective of this work was to perform genotypic, phenotypic correlations and path analysis under commercial yield of garlic bulbs, depending on morphological and agronomic variables. The experiment was carried out in 2017 at the Universidade Federal dos Vales do Jequitinhonha e Mucuri and Universidade Federal de Lavras, using ten garlic genotypes, eight trials and two commercial cultivars, Caçador and Jonas. We evaluated: plant height; number of leaves, total bulb number; total bulb mass, total bulb yield, marketable bulb yield and number of cloves per bulb. The genotypic and phenotypic correlations were positive and significant between marketable yield of bulbs with bulb mass, total yield and number of cloves per bulb, indicating that selection based on these characteristics will help increase the yield of garlic. However, only the number of cloves per bulb had a direct effect and a relevant indirect effect on the marketable yield of garlic.

https://doi.org/10.1590/s0102-053620190211 740 downloads

HISTOLOGICAL ANALYSIS OF INDIRECT SOMATIC EMBRYOGENESIS INDUCED FROM ROOT EXPLATS OF OIL PALM (Elaeis guineensis Jacq)
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Vilela, Marlucia Souza Pádua

; Andrade, Jessica de Castro e

; Santos, Raissa Silveira

; Stein, Vanessa Cristina

; Paiva, Luciano Vilela

Revista Árvore

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2019, Volume 43 N. 1 elocation e430106

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO O dendezeiro é economicamente importante pela alta produção de óleo. A embriogênese somática indireta em dendezeiro demanda longo tempo de indução de calos e formação das plantas, sendo importante o estudo do potencial embriogênico dos calos e os mecanismos da embriogênese somática. O objetivo deste estudo foi verificar o efeito de diferentes reguladores de crescimento e espermina na indução de calos embriogênico em explantes de raízes de dendezeiro. Explantes apicais radiculares de aproximadamente 0,5 cm foram isolados de plantas cultivadas in vitro e inoculadas em meio de cultura Y3 com os seguintes tratamentos: meio A - sem reguladores de crescimento; B - 1 mg.L-1 picloram (ácido 4-amino-3,5,6-tricloro-2-piridinacarboxílico); C- 1 mg.L-1 de picloram e 2 mg.L-1 de 2ip (2-isopenteniladenina); D - 2 mg.L-1 de 2ip; E - 1 mg.L-1 de picloram e 2 mg.L-1 de BAP (6-benzilaminopurina); F - 2 mg.L-1 de BAP; e G - 14,5mg.L-1 de espermina. Após, seis meses de cultivo, foram realizadas analises histológicas. Os calos induzidos nos tratamentos com 1 mg.L-1 picloram (B) e 14,5 mg.L-1 espermina (G) apresentaram características embriogênicas, células pequenas, isodiamétricas e formando aglomerados, apesar da grande quantidade de amido. Os calos do melhor tratamento (Y3 com 1 mg.L-1 de picloram) foram inoculados em meio de cultura de Y3 sem adição de reguladores de crescimento para sua regeneração, que após oito meses foram realizadas novas análises histológicas. Todos os tratamentos apresentaram formação de calo, com exceção dos tratamentos D e A. Os calos do tratamento B exibiram células com características embriogênicas, que desenvolveram embriões somáticos.

ABSTRACT Oil palm is economically important as a crop with high oil production. Indirect somatic embryogenesis in oil palm requires a long time for callus induction and plant formation, and it is important to study the embryogenic potential of calli and the mechanisms of somatic embryogenesis. The aim of this study was to test different growth regulators and spermine in induction of embryogenic calli in root explants of oil palm (Elaeis guineensis Jacq). Apex root explants of approximately 0.5 cm were isolated from plants cultivated in vitro and inoculated in Y3 culture medium in the following treatments: A - without growth regulators; B - 1 mg.L-1 picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid); C - 1 mg.L-1 picloram and 2 mg.L-1 2ip (2-isopentenyladenine); D - 2 mg.L-1 2ip; E - 1 mg.L-1 picloram and 2 mg.L-1 BAP (6-benzylaminopurine); F - 2 mg.L-1 BAP; and G - 14.5 mg.L-1 spermine. After six months of culturing, the calli induced in the treatments were analyzed by light microscopy. The calli induced in the treatments with 1 mg.L-1picloram (B) and treatment with 14.5 mg.L-1spermine (G) exhibited embryogenic characteristics, small and isodiametric cells, forming agglomerates, besides a large amount of starch. Calli of the best treatment (Y3 com 1 mg.L-1 de picloram) were inoculated in Y3 culture medium without addition of growth regulators. After eight months, calli were once more analyzed under light microscopy. All the treatments showed callus formation, except for treatments D and A. Calli of treatment B exhibited cells with embryogenic characteristics that developed somatic embryos.

https://doi.org/10.1590/1806-90882019000100006 906 downloads

Comparative study of fibrosis induced by Marlex®, Parietex Composite®, Vicryl® and Ultrapro® meshes1
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Biondo-Simões, Maria de Lourdes Pessole

; Sichciopi, Arthur Andrade

; Ioshii, Sérgio Ossamu

; Robes, Rogério Ribeiro

; Biondo-Simões, Rachel

Acta Cirúrgica Brasileira

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sep 2018, Volume 33 N. 9 Pages 792 - 798

Abstract: En | Text: En | PDF: En

Abstract Purpose: To evaluate the fibrosis induced by four different meshes: Marlex®, Parietex Composite®, Vicryl® and Ultrapro®. Methods: Histological cutouts of abdominal wall were analyzed with polarized light 28 days after the meshes implants and colorized by picrosirius to identify the intensity of collagen types I and III, and their maturation index. Results: When the four groups were compared, the total collagen area analyzed was bigger in groups A and D, with no difference between them. The collagen type I density was bigger in group A, with an average of 9.62 ± 1.0, and smaller in group C, with an average of 3.86 ± 0.59. The collagen type III density was similar in groups A, B and C, and bigger in group D. The collagen maturation index was different in each of the four groups, bigger in group A with 0.87, group B with 0.66, group D with 0.57 and group C with 0.33 (p = 0.0000). Conclusion: The most prominent fibrosis promotion in the given meshes was found on Marlex® (polypropylene mesh) and the Parietex Composite® (non-biodegradable polyester); the collagen maturation index was higher in the Marlex® mesh, followed by Ultrapro®, Parietex Composite® and Vicryl® meshes.

https://doi.org/10.1590/s0102-865020180090000007 986 downloads

EFFECTS OF ELASTIC WRAP ON MUSCLE ACTIVITY AND ISOMETRIC FORCE OF KNEE EXTENSORS
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Freitas, Fábio Sisconeto de

; Gomes, Willy Andrade

; Marchetti, Paulo Henrique

Revista Brasileira de Medicina do Esporte

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aug 2018, Volume 24 N. 4 Pages 295 - 299

Abstract: Pt En Es | Text: Pt En Es | PDF: Pt | PDF: En | PDF: Es

RESUMO Introdução: A banda elástica de joelhos (BEJ) é um equipamento utilizado por powerlifters, weightlifters e sujeitos treinados em força, com o objetivo de estabilizar a articulação do joelho e aumentar o desempenho da força (efeito carry over). Objetivo: Comparar os efeitos do uso da banda elástica de joelhos na atividade muscular de vasto lateral (VL) e na força de extensores de joelhos, durante a contração voluntária máxima isométrica (CVMI) na cadeira extensora. Métodos: Participaram do estudo 18 sujeitos saudáveis (idade: 25,56 ± 4,84 anos, massa corporal total: 81,83 ± 8,73 kg, estatura: 176 ± 5,17 cm), do gênero masculino, treinados em força (> 1 ano). Foram realizadas três contrações voluntárias máximas (CVMI's de extensores de joelho, de cinco segundos por 10 segundos de intervalo entre as tentativas, para cada condição experimental: controle (C); com banda elástica de joelhos (BEJ); e com tensor (T). Foram avaliados o desempenho de extensores de joelho e a atividade muscular de VL. Uma ANOVA com medidas repetidas foi utilizada para comparar as variáveis dependentes (carry over; pico de força, IEMG e Impulso). Resultados: Para o carry over, o uso da BEJ apresentou maior valor (74,21%) quando comparada a condição controle (P<0,001, d=0,96) e 79,37% maior do que o tensor (P<0,001, d=0,97). O pico de força foi 12,2% maior na condição C comparado à BEJ (P=0,018, d=0,71). Para o impulso, a condição controle apresentou um aumento de 8% comparado ao uso da BEJ (P=0,015, d=0,62). Para a IEMG não foram observadas diferenças significantes. Conclusão: O uso da banda elástica de joelhos aumentou a capacidade de produção de força durante a extensão de joelho. A remoção do efeito mecânico proveniente do carry over, durante a CVMI, reduz o desempenho de força dos extensores de joelho, mas não a ativação muscular do VL. Nível de Evidência I; Estudos terapêuticos - Investigação dos resultados do tratamento.

ABSTRACT Introduction: The elastic knee wrap (EKW) is a device used by powerlifters, weightlifters and subjects undergoing strength training with the aim of stabilizing the knee joint and increasing the development of strength (carryover effect). Objective: To compare the effects of elastic knee wrap use on muscular activity of the vastus lateralis (VL) and on knee extension force during maximum voluntary isometric contraction (MVIC) on the knee extensor chair. Methods: The study included 18 healthy male strength trained (>1 year) subjects (age: 25.56 ± 4.84 years, total body mass: 81.83 ± 8.73 kg, height: 176 cm ± 5.17). Three five-second maximum voluntary isometric contractions (MVICs) of the knee extensors were performed with a 10-second interval between attempts, for each experimental condition: control (C), with elastic knee wrap (EKW), and with tensor (T). The performance of the knee extensors and VL muscle activity were evaluated. A repeated measures ANOVA was used to compare the dependent variables (carryover, peak force, IEMG and Impulse). Results: For carryover, the use of EKW presented a higher value (74.21%) than the control condition (p <0.001, d = 0.96) and was 79.37% higher than the tensor (p <0.001, d = 0.97). Peak force was 12.2% higher in condition C compared to EKW (p = .018, d = 0.71). For impulse, the control condition presented an increase of 8% compared to EKW use (p = .015, d = 0.62). No significant differences were observed for IEMG. Conclusion: Elastic knee wrap use increased strength production capacity during knee extension. The removal of the mechanical effect originating from carryover during MVIC reduces the strength performance of the knee extensors, but not the muscular activation of the VL. Level of Evidence I; Therapeutic studies - Investigation of treatment results.

RESUMEN Introducción: La venda elástica de rodillas (EKW) es un dispositivo utilizado por powerlifters, weightlifters y sujetos a entrenados en fuerza con el objetivo de estabilizar la articulación de rodilla y para aumentar el desarrollo de la fuerza (efecto carry over). Objetivo: Comparar los efectos del uso de venda elástica de rodillas en actividad muscular del vasto lateral (VL) y la fuerza de los extensores de rodilla durante la contracción máxima isométrica voluntaria (MVIC) en la silla extensora. Métodos: El estudio incluyó a 18 sujetos saludables (edad: 25,56 ± 4,84 años, masa corporal total: 81,83 ± 8,73 kg, altura: 176 cm ± 5,17), sexo masculino, entrenado en fuerza (> 1 año). Fueron realizadas tres contracciones máximas voluntarias extensoras de rodilla (MVIC), de cinco segundos por 10 segundos de intervalo entre intentos para cada condición experimental: control (C), con las vendas eláticas de rodillas (EKW), y con tensor (T). Fueron evaluados el desempeño de los extensores de rodilla y la actividad muscular de VL. Una ANOVA con medidas repetidas fue utilizada para comparar las variables dependientes (carry over, el tope de fuerza, IEMG e Impulso). Resultados: Para el carry over, el uso de EKW presentó un valor de mayor (74,21%) en comparación con la condición de control (P <0,001, d = 0,96) y 79,37% mayor que el tensor (P <0,001, d = 0,97). El tope de fuerza con fue 12% mayor en la condición C, comparado EKW (P = .018, d = 0,71). Para el impulso, la condición control presentó un aumento del 8% comparado al uso de la EKW (P = .015, d = 0,62). Para IEMG no fueron observadas diferencias significativas. Conclusión: El uso de vendas elásticas de rodillas aumentó la capacidad de producción de fuerza durante la extensión de rodilla. La remoción del efecto mecánico proveniente del carry over durante la MVIC reduce el desempeño de fuerza de los extensores de rodilla, pero no la activación muscular del VL. Nivel de Evidencia I; Estudios terapéuticos - Investigación de los resultados del tratamiento.

https://doi.org/10.1590/1517-869220182404166615 1100 downloads

Isolation and genotyping of Clostridium perfringens from goats in Minas Gerais, Brazil
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Santana, Jordana Almeida

; Ferreira, Ana Carolina de Andrade

; Souza, Marina de Castro Campos de

; Moreira, Maria Aparecida Scatamburlo

; Lima, Magna Coroa

; Cruz, Diogo Soares Gonçalves

; Lobato, Francisco Carlos Faria

; Silva, Rodrigo Otávio Silveira

Ciência Rural

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2018, Volume 48 N. 7 elocation e20180101

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: Apesar da reconhecida importância de Clostridium perfringens como enteropatógeno de pequenos ruminantes, pouco se sabe sobre a frequência dos genótipos ou do papel de fatores de virulência adicionais de C. perfringens em cabras saudáveis, dificultando o diagnóstico laboratorial da infecção causada por esse micro-organismo. Dessa forma, o presente estudo teve como objetivo caracterizar C. perfringens de amostras de fezes de cabras adultas saudáveis. Amostras de fezes de 250 cabras saudáveis de 17 rebanhos diferentes em Minas Gerais, Brasil, foram submetidas ao isolamento e genotipagem de C. perfringens. C. perfringens tipo A foi isolado de 189 (75,6%) cabras, enquanto C. perfringens tipos C e D foram detectados em um animal (0.4%) cada. Todos os isolados foram negativos para os genes codificadores das toxinas NetB, NetE, NetF e enterotoxina. Os resultados apresentados confirmam C. perfringens tipo A como parte da microbiota de cabras saudáveis e sugere que C. perfringens tipos C e D são raramente encontrados em caprinos saudáveis.

ABSTRACT: Despite the known importance of Clostridium perfringens as an enteropathogen in small ruminants, little is known about the role of its additional virulence factors or the frequency of the various C. perfringens genotypes in healthy goats; this complicates the laboratory diagnosis of the infections caused by this microorganism. In light of this, the aim of the present study was to isolate and genotype C. perfringens from stool samples from healthy goats in Brazil. Stool samples from 250 apparently healthy adult goats from 17 different herds in Minas Gerais, Brazil were collected, and isolation and genotyping of C. perfringens was performed. C. perfringens type A was isolated from 189 (75.6%) goats, whereas C. perfringens types C and D were each detected in one goat (0.4%). All isolates were negative for enterotoxin-, NetB-, NetE-, and NetF-encoding genes. These results confirmed C. perfringens type A as part of the microbiota in these animals, and they suggested that C. perfringens type C and D are rarely isolated from healthy goats.

https://doi.org/10.1590/0103-8478cr20180101 1110 downloads

10.

Degradación ruminal de dietas a base de biomasa pos-cosecha de amaranthus cruentus: efecto sobre los protozoos del rumen y producción de gas in vitro
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Barros-Rodríguez, M

; Oña-Rodríguez, J

; Mera-Andrade, R

; Artieda-Rojas, J

; Curay-Quispe, S

; Avilés-Esquivel, D

; Solorio-Sánchez, J

; Guishca-Cunuhay, C

Revista de Investigaciones Veterinarias del Perú

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oct 2017, Volume 28 N. 4 Pages 812 - 821

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

The aim of this research was to evaluate the in situ rumen degradation kinetics of nutrients, as well as the apparent digestibility of nutrients, population of rumen protozoa, and in vitro gas production of based diets from post-harvest biomass of Amaranthus cruentus. Post-harvest biomass of A. cruentus with Pennisetum clandestinum forage was mixed in dry basis according to four treatments: T1: 100% A. cruentus, T2: 100% P. clandestinum, T3: 75% P. clandestinum + 25% A. cruentus, T4: 50% P. clandestinum + 50% A. cruentus, under a completely randomized design. The ruminal degradation parameters showed differences between treatments (p<0.05) obtaining the highest effective degradation of dry matter (DM), crude protein (CP) and neutral detergent fibre (NDF) in T1 (423.4, 404.1 and 354.5 g/kg, respectively). The in vitro digestibility of DM, organic matter (OM) and CP was higher (p<0.05) in T1 (60, 68 and 59% respectively) and T4 (53, 61 and 51% respectively). The lowest in vitro gas production was for T1, with a difference of approximately 91 ml/0.5 mg fermented DM with respect to the other treatments (p=0.0001). The lowest (p=0.0026) population of protozoa Holotrichs and Entodinomorphs was observed in T1 (0 and 1.80 log10 respectively). It is concluded that the post-harvest biomass of A. cruentus when incorporated into the diet of ruminants, can increase the degradation and digestibility of nutrients, and reduce the production of greenhouse gases and populations of protozoa of the rumen.

El objetivo de esta investigación fue evaluar la cinética de degradación ruminal de los nutrientes in situ, así como la digestibilidad aparente de los nutrientes, población de protozoarios del rumen y producción de gas in vitro de dietas a base de biomasa pos-cosecha de Amaranthus cruentus. La biomasa pos-cosecha del A. cruentus con el forraje de Pennisetum clandestinum se mezcló en base seca de acuerdo a los siguientes tratamientos: T1: 100% A. cruentus, T2: 100% P. clandestinum, T3: 75% P. clandestinum + 25% A. cruentus, T4: 50% P. clandestinum + 50% A. cruentus, bajo un diseño completamente aleatorizado. Los parámetros de degradación ruminal muestran diferencias entre tratamientos (p<0.05), obteniendo la mayor degradación efectiva de la materia seca (MS), proteína cruda (PC) y fibra detergente neutro (FDN) en T1 (423.4, 404.1 y 354.5 g/kg MS, respectivamente). La digestibilidad in vitro de la MS, materia orgánica (MO) y PC fue mayor (p<0.05) en T1 (60, 68 y 59%, respectivamente) y T4 (53, 61 y 51%, respectivamente). La menor producción de gas in vitro fue para T1, con una diferencia aproximada de 91 ml/0.5 g MS fermentable con respecto a los demás tratamientos (p=0.0001). La menor (p=0.0026) población de protozoarios Holotricos y Entodiniomorfos se observó en T1 (0 y 1.80 log10, respectivamente). Se concluye que la biomasa pos-cosecha de A. cruentus al incorporarlo a la dieta de los rumiantes puede incrementar la degradación y digestibilidad de los nutrientes y reducir la producción de gases de efecto invernadero y las poblaciones de protozoarios del rumen.

https://doi.org/10.15381/rivep.v28i4.13931

11.

Surto de leptospirose em bezerros criados em resteva de arroz
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Reis, Matheus de Oliveira

; Caprioli, Rafaela A.

; Laisse, Cláudio João M.

; Guimarães, Lorena L.B.

; Andrade, Caroline P. de

; Boabaid, Fabiana M.

; Sonne, Luciana

; Driemeier, David

Pesquisa Veterinária Brasileira

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sep 2017, Volume 37 N. 9 Pages 937 - 940

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO: A leptospirose é uma doença infecciosa causada por bactérias do gênero Leptospira, que afeta animais domésticos, selvagens e também humanos. De outubro a novembro de 2014, numa propriedade rural localizada em Glorinha, RS, em que bovinos eram mantidos em resteva de arroz, 13 bezerros manifestaram hemoglobinúria e apatia, nove dos quais morreram em menos de 24 horas após o início dos sinais clínicos. Foram necropsiados quatro bezerros (A, B, C e D). Fragmentos de tecido foram fixados em formalina a 10%. Amostras de rim, fígado e pulmão dos Bezerros B, C e D foram enviadas para análise de PCR para RNA ribossômico 16S e a proteína Lip 32 de Leptospira. No exame macroscópico foram observados mucosas e tecido subcutâneo amarelados, fígado alaranjado, pulmões com múltiplas petéquias, predominantemente nos lóbulos craniais. A cavidade torácica do Bezerro A estava repleta de um líquido vermelho-escuro. À avaliação microscópica foi observada hemorragia acentuada nos pulmões; no fígado havia necrose e vacuolização hepatocelular centrolobular difusa moderada, além de infiltrado linfocítico periportal discreto. Nos rins observou-se nefrite intersticial linfoplasmocítica discreta multifocal. A análise por PCR teve resultado positivo para os Bezerros B e D. O diagnóstico de leptospirose nos bezerros foi baseado nos achados epidemiológicos, clínicos e patológicos, associados ao resultado positivo na PCR. Este estudo demonstra a importância da investigação da doença quando animais jovens são criados em áreas inundadas e têm manifestações clínicas de doença septicêmica aguda.

ABSTRACT: Leptospirosis is an infectious disease caused by bacteria of the genus Leptospira, which affect domestic and wild animals, and also humans. From October to November 2014, in a rural property located in Glorinha, RS, where cattle were kept in the rice stubble, thirteen calves presented hemoglobinuria and apathy, nine of which died within less than 24 hours after the onset of clinical signs. Four calves were necropsied (A, B, C and D). Tissue samples were collected in 10% formalin. Samples of kidney, liver and lung from calves B, C and D were sent for PCR analysis for 16S ribosomal RNA and the protein Lip 32 genes of Leptospira. At macroscopic examination jaundiced mucosae and subcutaneous tissue, orange liver, and lungs with multiple petechiae, predominantly in cranial lobes, were observed. The thoracic cavity of calf A was filled with a reddish fluid. At microscopic examination, severe hemorrhage was observed in the lungs; in the liver there was moderate diffuse centrilobular hepatocellular necrosis and vacuolization, in addition to discrete periportal lymphocytic infiltrate. Discrete multifocal lymphoplasmocytic interstitial nephritis was observed in the kidneys. PCR analyzis resulted positive for calves B and D. The diagnosis of leptospirosis in the calves was based on epidemiological, clinical and pathological findings associated with positive PCR analysis. This study demonstrates the importance of investigation of the disease when young bovids are raised in flooded areas and have clinical signs of an acute septicemic disease.

https://doi.org/10.1590/s0100-736x2017000900007 2658 downloads

12.

Eficiência da estimação da área foliar de couve por meio de redes neurais artificiais
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Azevedo, Alcinei M

; Andrade Júnior, Valter C

; Sousa Júnior, Aderbal S

; Santos, Albertir A

; Cruz, Cosme D

; Pereira, Samuel L

; Oliveira, Altino JM

Horticultura Brasileira

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mar 2017, Volume 35 N. 1 Pages 14 - 19

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

RESUMO A estimativa da área foliar na couve é importante, pois medidas diretas são difíceis e imprecisas, devido ao tamanho da folha, a irregularidade da superfície foliar de alguns genótipos, a necessidade de equipamentos caros e de muita mão-de-obra. Objetivou-se verificar a eficiência da estimação da área foliar de couve por meio de RNAs e constatar a eficiência desta estratégia em comparação com o uso da área foliar observada. O experimento foi conduzido em delineamento de blocos casualizados com três repetições, 22 acessos e quatro plantas por parcela. Desenvolveram-se perceptrons de multicamadas utilizando 50 folhas por acesso, destinando-se 70% para treinamento, 15% para a validação cruzada (early-stop) e 15% para teste. Foram testadas 39 configurações de rede perceptron de multicamadas. As RNAs foram eficientes para estimar a área foliar da couve a partir do comprimento e largura do limbo foliar. A área foliar estimada pela RNA é indicada para a seleção de plantas por ser de fácil obtenção, ser um método não destrutivo, apresentar alta correlação fenotípica e genética com a área foliar observada e maior herdabilidade.

ABSTRACT The estimation of leaf area in kale is important because direct measurements are difficult and inaccurate, due to the leaf size, the irregularity of the leaf surface of some genotypes, the need for expensive equipment and intensive labor. The objective was to verify the efficiency of artificial neural networks to estimate the leaf area and verify the efficiency of the use of the estimated area in the selection process compared with the observed area. The experiment was conducted in a randomized block design with three replications, 22 accesses and four plants per plot. Multilayer perceptrons were developed using 50 leaves per access, 70% designed for training, 15% for cross-validation (early-stop) and 15% for testing. 39 perceptron multilayer network settings were tested. The RNAs were efficient to estimate leaf area from the length and width of the leaf blade. The leaf area estimated by the RNA is indicated for the selection of plants due to its easily access and due to be a non-destructive method, having high phenotypic and genetic correlation with leaf area observed and higher heritability.

https://doi.org/10.1590/s0102-053620170103 1963 downloads

13.

Efecto del pH, Sólidos Solubles y Zumo Adicionado sobre el color y la Vitamina C de Zumo de Naranja Agria Cocristalizado
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Andrade, Ricardo D

; Blanquicett, Kevin

; Rangel, Rafael D

Información tecnológica

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2016, Volume 27 N. 6 Pages 129 - 134

Abstract: En Es | Text: En Es | PDF: En | PDF: Es

The effect of the proportion of juice added (10 and 20%), pH (3.8 and 4.5), and soluble solids (50 and 60 ° Brix) on time co-crystallization, color and vitamin C content of co-crystals of bitter orange juice was evaluated. Bitter co-crystallized orange juice was obtained by mixing supersaturated sucrose syrup at 118 °C with bitter orange juice concentrate, with constant agitation of 600 rpm. The wet co-crystals were separated and dried at 50 °C in a vacuum oven for 24 hours. Color and vitamin C content were determined in the dry co-crystals. Co-crystals managed to retain vitamin C between 3.94 and 5.25 mg/100g dry basis. However, there was high loss of vitamin C (90.64%) during the process of co-crystallization with respect to fresh juice.

El objetivo de este estudio fue evaluar el efecto de la proporción de jugo adicionado (10 y 20%), pH (3.8 y 4.5), y sólidos solubles del zumo (50 y 60 °Brix) sobre el tiempo de cocristalización, el color y contenido de vitamina C de los cocristales de zumo de naranja agria. El zumo de naranja agria cocristalizado fue obtenido mezclando jarabe de sacarosa sobresaturada a 118°C con zumo de naranja agria concentrado, con agitación constante de 600 rpm. Los cocristales húmedos fueron separados y secados a 50°C en una estufa al vacío por 24 horas. A los cocristales secos se les determinó color y vitamina C. Los cocristales lograron retener vitamina C entre un 3.94 y 5.25 mg/100 g en base seca. Sin embargo, se presentó una alta pérdida de vitamina C (90.64%) durante el proceso de cocristalización con respecto al zumo fresco.

15953 downloads

14.

Experimental use of a cellulosic biopolymer as a new material for suburethral sling in the treatment of stress urinary incontinence
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Lucena, Roberto G.

; Lima, Salvador V. C.

; Aguiar, José L. de A.

; Andrade, Rogerson T.

; Pinto, Flávia C. M.

; Vilar, Fabio O.

International braz j urol

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dec 2015, Volume 41 N. 6 Pages 1148 - 1153

Abstract: En | Text: En | PDF: En

Purpose: To analyze the interaction between the cellulose exopolysaccharide (CEC) and urethral tissue when used as a pubovaginal sling. Materials and Methods: Forty Wistar rats were divided into four groups. In groups A and B the cellulose exopolysaccharide (CEC) was implanted around the urethral tissue (bladder neck below the upper margin) and the rats were sacrificed at 30 and 90 days. Similar procedure was used in groups C and D using a polypropylene mesh. After sacrifice bladder and urethra were sent for histological analysis. The histological parameters (inflammatory reaction) by evaluated by quantitative analysis. For collagen deposition analysis it was used stereological method. Results: The cellulose exopolysaccharide (CEC) was inert and well preserved at the implanted region at the time of examination. Morphologic alterations were not found at the CEC implant but some reactions of foreign body type were observed at the adjacent structures. In some areas a process of neovascular formation was observed. Stereological analysis at the suburethral area showed a significant difference in collagen presence in favor of CEC. Conclusions: The CEC implant showed adequate results when used as a suburethral sling with good integration to the host tissue, preserving its architecture.

https://doi.org/10.1590/S1677-5538.IBJU.2014.0155 943 downloads

15.

V Diretriz da Sociedade Brasileira de Cardiologia sobre Tratamento do Infarto Agudo do Miocárdio com Supradesnível do Segmento ST
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Piegas, LS

; Timerman, A

; Feitosa, GS

; Nicolau, JC

; Mattos, LAP

; Andrade, MD

; Avezum, A

; Feldman, A

; De Carvalho, ACC

; Sousa, ACS

; Mansur, AP

; Bozza, AEZ

; Falcão, BAA

; Markman Filho, B

; Polanczyk, CA

; Gun, C

; Serrano Junior, CV

; Oliveira, CC

; Moreira, D

; Précoma, DB

; Magnoni, D

; Albuquerque, DC

; Romano, ER

; Stefanini, E

; Santos, ES

; God, EMG

; Ribeiro, EE

; Brito Júnior, FS

; Feitosa-Filho, GS

; Arruda, GDS

; Oliveira, GBF

; Lima, GG

; Dohmann, HFR

; Liguori, IM

; Costa, JR

; Saraiva, JFK

; Maia, LN

; Moreira, LFP

; Arrais, M

; Canesin, MF

; Coutinho, MSSA

; Moretti, MA

; Ghorayeb, N

; Vieira, NW

; Dutra, OP

; Coelho, OR

; Leães, PE

; Rossi, PRF

; Andrade, PB

; Lemos, PA

; Pavanello, R

; Vivacqua Costa, RC

; Bassan, R

; Esporcatte, R

; Miranda, R

; Giraldez, RRCV

; Ramos, RF

; Martins, SK

; Esteves, VBC

; Mathias Junior, W

Arquivos Brasileiros de Cardiologia

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aug 2015, Volume 105 N. 2 Pages 1 - 121

Abstract: Pt | Text: Pt | PDF: Pt

https://doi.org/10.5935/abc.20150107 181064 downloads

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