ABSTRACT Animal-pollinated flowers may orient resources for competing activities, such as nectar production for attracting flower visitors but then saving nectar (through inhibiting nectar production or by final resorption) for the subsequent maturation of fruits and seeds. Nectar production is continuous in Nicotiana longiflora and N. alata after flower opening, but early nectar removal reduces total secreted nectar. Resource trade-off between nectar investment and seed production were experimentally assessed in manually pollinated flowers experiencing different numbers of repeated nectar removals, while controlling for maternal effects. We expected that flowers with less nectar secretion produce larger seed sets. The results showed that for both species the earlier the nectar removal during flower anthesis, the lower the total nectar secreted and the higher the mass of seeds produced. This general pattern was clearer for N. longiflora. The link between decreased nectar production and the subsequent increase in the seed set implies that resources are limited. Consequently, nectar savings during the pollination process through early nectar removal by pollinators can be interpreted as a trade-off between resources secreted by flowers for pollinator attraction and those utilized during fruit and seed maturation.