Abstract In a previous study, we reported the antispasmodic and gastroprotective effects of the Serjania caracasana (Jacq.) Willd., Sapindaceae, extract. In the present study, we evaluated the LD50, hemolytic and antispasmodic activities of its fractions and characterized its major constituents by isolation and GC–MS. The animals showed non-toxic symptoms with oral doses up to 2000 mg/kg, suggesting a safe oral administration. Furthermore, a low hemolytic activity was detected for the saponin fraction. Antispasmodic activity of the fractions was evaluated through carbachol-induced contractions in rat ileum. The hexane fraction was the most potent (IC50 68.4 ± 5.9 µg/ml) followed by the dichloromethane fraction (IC50 161.3.4 ± 40.7 µg/ml). Butanol fraction was the less effective (IC50 219.8 ± 60.3 µg/ml). The phytochemical study of the S. caracasana fractions afforded the isolation of friedelin, β-amyrin, allantoin and quercitrin. This is the first time that the presence of allantoin and quercitrin in the Serjania genus has been reported. Among the isolated compounds and those characterized by GC–MS, β-amyrin and β-sitosterol were present in the most active fractions, hexane and dichloromethane, and they may be related to its antispasmodic activity. In addition, spathulenol was only found in the hexane fraction and its presence might justify the highest antispasmodic activity observed for this fraction.

O objetivo do trabalho foi avaliar os efeitos da origem em diferentes densidades populacionais de Typha angustifolia nas características anatômicas e fisiológicas. As plantas foram coletadas de populações muito (acima de 50% da capacidade de colonização) e pouco adensadas (abaixo de 50% da capacidade de colonização), sendo cultivadas sob condições controladas em casa de vegetação. As plantas de ambas as populações foram cultivadas em bandejas de plástico contendo 4 L de solução nutritiva por 60 dias. Ao final desse período, foram avaliadas a taxa de crescimento relativo, razão de área foliar, taxa assimilatória líquida, razão raiz/parte aérea, anatomia foliar e radicular, atividade da catalase e peroxidase do ascorbato. As plantas das populações muito adensadas mostraram maiores taxas de crescimento e razão raiz/parte aérea. Populações pouco adensadas apresentaram maiores valores para o índice e densidade estomáticos foliares, bem como maior espessura do parênquima paliçádico. A espessura da epiderme e exoderme nas raízes, bem como a proporção de aerênquima das populações muito adensadas foram menores, sendo que essas plantas também apresentaram maiores valores para a proporção de cilindro vascular. Somente a atividade da catalase foi diferente entre as populações, apresentando maiores valores para as populações pouco adensadas. Portanto, plantas de Typha angustifolia apresentam diferenças em sua anatomia e fisiologia relacionadas a sua origem em condições de alto ou baixo adensamento populacional. As populações muito adensadas apresentam maior capacidade de crescimento, possivelmente relacionada a reduções nas barreiras apoplásticas podendo estar associadas à maior capacidade da absorção de nutrientes.

The objective of this work was to evaluate the effects of the population density of Typha angustifolia plants in the anatomical and physiological characteristics. Plants were collected from populations of high density (over 50% of colonization capacity) and low density (less than 50% of colonization capacity) and cultivated under controlled greenhouse conditions. Plants from both populations were grown in plastic trays containing 4 L of nutritive solution for 60 days. At the end of this period, the relative growth rate, leaf area ratio, net assimilatory rate, root/shoot ratio, leaf anatomy, root anatomy, and catalase and ascorbate peroxidase activities were evaluated. Plants from high density populations showed increased growth rate and root/shoot ratio. Low density populations showed higher values of stomatal index and density in leaves, as well as increased palisade parenchyma thickness. Root epidermis and exodermis thickness as well as the aerenchyma proportion of high density populations were reduced, these plants also showed increased vascular cylinder proportion. Only catalase activity was modified between the high and low density populations, showing increased values in low density populations. Therefore, different Typha angustifolia plants show differences in its anatomy and physiology related to its origins on high and low density conditions. High density population plants shows increased growth capacity related to lower apoplastic barriers in root and this may be related to increased nutrient uptake capacity.

Sugere-se uma alteração no método de amostragem da cigarrinha-da-raiz para a cultura da cana-de-açúcar com o objetivo de aprimorar o controle da praga. O monitoramento convencional de Mahanarva fimbriolata pode subestimar o número de ovos da área, ocasionando ressurgência da praga. Neste sentido, um estudo foi realizado em Ponta Porã, MS, com o objetivo de alterar a proposta de amostragem da M. fimbriolata. O delineamento experimental composto por blocos casualizados, com dez tratamentos e quatro repetições. Os tratamentos testados foram: 1) Testemunha - não tratada, 2) Thiamethoxam 250WG - 250 g ha-1, 3) IBCB 425 - 5 x 10(9) con ha-1, 4) IBCB 425 - 10 x 10(9) con ha-1, 5) IBCB 425 - 15 x 10(9) con ha-1, 6) BIO 08 - 5 x 10(9) con ha-1, 7) BIO 08 - 10 x 10(9) con ha-1, 8) BIO 08 - 15 x 10(9) con ha-1, 9) IBCB 425 - 5 x 10(9) con ha-1 - aplicação granulada e 10) BIO 08 - 5 x 10(9) con ha-1 - aplicação granulada. As avaliações de eficiência dos tratamentos obtidas aos 15, 45, 75 e 105 dias após a aplicação (DAA). Aos 15 DAA os tratamentos com os isolados BIO 08 - aplicação granulada e IBCB 425 nas doses de 5 x 10(9) con ha-1 e 10 x 10(9) con ha-1 apresentaram eficiência de 73,33 e 70,66%, respectivamente. Não houve diferenças significativas entre os tratamentos com os inseticidas biológicos e químicos testados no experimento aos 75 e 105 DAA.

The present study suggests a change in the method of sampling the spittlebug in sugarcane crops in order to improve the control of this pest. Conventional monitoring of Mahanarva fimbriolata may underestimate the number of eggs in the area, causing pest resurgence. In this regard a study was carried out in Ponta Porã, MS, Brazil, in order to study possible changes in the methods used for sampling M. fimbriolata. The experiment consists of randomized blocks with 10 treatments and 4 replications. The treatments were: 1) control - untreated, 2) Thiamethoxam 250 WG at 250 g ha-1, 3) IBCB 425 at 5 x 10(9) con ha-1, 4) IBCB 425 at 10 x 10(9) con ha-1, 5) IBCB 425 at 15 x 10(9) con ha-1, 6) BIO 08 at 5 x 10(9) con ha-1, 7) BIO 08 at 10 x 10(9) con ha-1, 8) BIO 08 at 15 x 10(9) con ha-1, 9) IBCB 425 at 5 x 10(9) con ha-1 - granular application and 10) BIO 08 at 5 x 10(9) con ha-1 - granular application. Evaluations of effectiveness of treatments were obtained at 15, 45, 75 and 105 days after application (DAA). At 15 DAA treatments with the isolated application with grainy BIO 08 and IBCB 425 at dosage of 5 x 10(9) con ha-1 and 10 x 10(9) con ha-1 showed an efficiency of 73.33 and 70.66%, respectively. There were no significant differences between treatments with biological and chemical insecticides tested in the experiment at 75 and 105 DAA.

Foram avaliadas, em duas gerações, a reprodução e a longevidade de Supputius cincticeps (Stål) (Heteroptera: Pentatomidae) alimentado com larvas de Zophobas confusa Gebien, Tenebrio molitor L. (Coleoptera: Tenebrionidae) ou Musca domestica (L.) (Diptera: Muscidae) a 24,7 ± 1,1ºC, 70 ± 10% de U.R. e fotofase de 12 h. O peso de adultos recém emergidos, o período de oviposição, o número de posturas, de ovos totais e a longevidade de fêmeas de S. cincticeps foram maiores com larvas de Z. confusa ou T. molitor que com M. domestica. Independentemente do tipo de presa, S. cincticeps mostrou melhor performance reprodutiva e longevidade na segunda geração.

Reproduction and longevity of Supputius cincticeps (Stål) (Heteroptera: Pentatomidae) fed on Zophobas confusa Gebien, Tenebrio molitor L. (Coleoptera: Tenebrionidae) or Musca domestica (L.) (Diptera: Muscidae) larvae were studied during two generations at 24.7 ± 1.1ºC, 70 ± 10% R.H. and 12 h of photophase. Body weight of newly-emerged adults, oviposition period, number of egg masses, total number of eggs and longevity of S. cincticeps were higher when fed on Z. confusa or T. molitor larvae than on M. domestica larvae. Regardless of diet, S. cincticeps showed better reproduction and longevity in the second generation in laboratory conditions.

As part of a semidetailed pedologic survey, the physiographic analysis of the area comprising the La Unión, La Victoria, Toro and Obando municipalities (Valle del Cauca Department, Colombia) was conducted. The aim of this work was the evaluation of the use of the physiographic analysis and its classification according to the CIAF System. The area includes two physiographic provinces: the Depresión Cauca-Patía (Cauca-Patía Graben) and the Cordillera Occidental (Western Range). Aggradation processes predominate in the former while degradation ones prevail in the latter. In the plains, the elements which enabled the reconnaissance of the landscape units were as follows: drainage channels, land use, drainage conditions and pedogenic processes, which, in some units, manifested through colour changes observed by means of photointerpretation. In these areas, the relief was of minor importance because terrain variations are of little significance. In the mountain region, the elements which enabled the differentiation of the landscape units were as follows: landscape (relief and compound slopes), natural vegetation, rocks, drainage pattern, parent material and denudation. It is concluded that the applied method and the adopted physiographic classification were instrumental in the understanding of the processes originating these landscapes.

Dentro de un levantamiento edafológico semidetallado, se realizó un análisis fisiográfico del área entre los municipios de La Unión, La Victoria, Toro y Obando (Departamento Valle del Cauca, Colombia). El objetivo del trabajo fue la evaluación de la utilización de la metodología del análisis fisiográfico y su clasificación por el sistema CIAF. En el sector se encuentran dos provincias fisiográficas: la Depresión Cauca-Patía y la Cordillera Occidental. En la primera dominan los procesos agradacionales, mientras en la segunda predominan los degradacionales. En la zona plana, los elementos que permitieron el reconocimiento de las unidades de paisaje son: canales de drenaje, uso de la tierra, condición de drenaje y procesos pedogenéticos, que en algunas unidades se manifestaron a partir de cambios de tonalidades observadas por fotointerpretación. En estos sectores el relieve tuvo muy poca importancia debido a que los desniveles en el terreno son de baja significación. En el sector de mayor relieve los elementos que permitieron diferenciar las distintas unidades de paisaje fueron: la configuración de la superficie (relieve y pendientes compuestas), vegetación natural, rocas, patrón de drenaje, material parental y denudación. Se concluye que la metodología aplicada y la clasificación fisiográfica adoptada contribuyeron a entender los procesos que originaron estos paisajes.