ABSTRACT: Feathers are by-products that are generated in significant quantities by the poultry industry. Microbial bioconversion has been investigated as a promising strategy for the processing of feathers, since, along with the degradation of these keratinous materials, bioprocessing can result in value-added products. Thus, from the perspective of industrial microbiology, chicken feathers can be considered a raw material for obtaining microbial proteases. Within this context, this research investigated and characterized the production of extracellular proteases by Aspergillus sp., isolated from soil of the Amazon Rainforest. The enzymatic production was evaluated using several growth substrates (whole feathers, feather meal, human hair, casein, gelatin, peptone and chicken beaks). With highest enzyme production was obtained the feather meal (FM) and peptone. After 48 h of fermentation, FM degradation was 15.82%. The crude protease showed optimal activity at pH 5.0 and 37 °C and enzymatic activity was enhanced with the addition of 1 and 5 mM of CaCl2, MnSO4, KCl, MgSO4 and CuSO4. The detergents Tween 20 and Triton x-100, at concentrations 0.5 and 1% (v/v), tended to stimulate activity. The presence of 0.5 and 1% (v/v) of organic solvents (methanol, acetone, butanol, acetonitrile, isopropanol and DMSO), maintained the enzymatic activity. β-mercaptoethanolstimulated proteolytic activity in the enzymatic assays. This study suggested new direction for waste management with industrial applications giving rise to green technology for sustainable development.

RESUMO: As penas são subprodutos que são gerados em quantidades significativas pela indústria avícola. A bioconversão microbiana tem sido investigada como uma estratégia promissora para o processamento de penas, uma vez que, juntamente com a degradação desses materiais queratinosos, o bioprocessamento pode resultar em produtos de valor agregado. Assim, do ponto de vista da microbiologia industrial, as penas de frango podem ser consideradas matéria-prima para a obtenção de proteases microbianas. Dentro deste contexto, o objetivo deste trabalho foi investigar e caracterizar a produção de proteases extracelulares por Aspergillus sp., isolados de solo da floresta Amazônica. A produção enzimática foi avaliada utilizando diversos substratos de crescimento (penas inteiras, farinha de penas, cabelo humano, caseína, gelatina, peptona e bicos de frango). Com maior produção de enzima foi obtida a farinha de penas (FP) e peptona. Após 48 h de fermentação, a degradação da FP foi de 15.82%. A protease bruta mostrou atividade ótima em pH 5.0 e 37 0C e a atividade enzimática foi aumentada com a adição de 1 e 5 mM de CaCl2, MnSO4, KCl, MgSO4 e CuSO4. Os detergentes Tween 20 e Triton x-100, nas concentrações 0.5 e 1% (v/v), tenderam a estimular a atividade. A presença de 0.5 e 1% (v/v) dos solventes orgânicos (metanol, acetona, butanol, acetonitrila, isopropanol e DMSO), mantiveram a atividade enzimática. O β-mercaptoetanol estimulou a atividade proteolítica nos ensaios enzimáticos. Este estudo sugere uma nova direção para a gestão de resíduos com aplicações industriais dando origem à tecnologia verde para o desenvolvimento sustentável.

ABSTRACT Introduction: Rituximab (RTX) is a therapeutic option in pediatric difficult-to-treat idiopathic nephrotic syndrome (NS). We aimed to assess the efficacy and safety of RTX use in these patients. Method: A retrospective study of all patients with idiopathic NS treated with RTX was conducted in a pediatric nephrology division of a tertiary hospital. Demographic, anthropometric, clinical and analytical data were collected prior to treatment and at 6, 12, and 24 months. Results: Sixteen patients were included (11 males), with a median (25th–75th percentile, P25–P75) age at diagnosis of 2 (2.0–2.8) years. Fifteen were steroid-sensitive and 1 was steroid-resistant and sensitive to cyclosporine. The median age at administration of RTX was 10 (6.3–14.0) years. Throughout a median follow-up time of 2.5 (1.0–3.0) years, 6 (37.5%) patients achieved partial remission and 7 (43.8%) had no relapses and were not taking any immunosuppressants at the 24-month follow-up visit. Regarding complications, 1 patient presented persistent hypogammaglobulinemia. Compared with the 12-month period before RTX, there was a decrease in the median number of relapses at 6 and 12 months [3 (3.0–4.0) vs 0 (0–0.8) and 0.50 (0–1.0), respectively; p = 0.001] and in the daily steroids dose (mg/kg/day) at 6, 12, and 24 months [0.29 (0.15–0.67)vs [0.10 (0.07–0.13); p = 0.001], [0.12 (0.05–0.22); p = 0.005] and [0.07(0.04–0.18); p = 0.021]], respectively. There was also a reduction in the median BMI z score at 24 months [2.11 (0.45–3.70) vs. 2.93 (2.01–3.98); p = 0.049]. Conclusion: Our results confirm the efficacy and safety of RTX use in pediatric idiopathic NS and highlight its’ potential cardiometabolic benefits.

Resumo Introdução: Rituximabe (RTX) é uma opção terapêutica na síndrome nefrótica (SN) idiopática pediátrica de difícil tratamento. Visamos avaliar eficácia e segurança do uso de RTX nestes pacientes. Método: Realizou-se estudo retrospectivo de todos os pacientes com SN idiopática tratados com RTX, em uma unidade de nefrologia pediátrica de um hospital terciário. Dados demográficos, antropométricos, clínicos e analíticos foram coletados antes do tratamento e aos 6, 12 e 24 meses. Resultados: Incluímos 16 pacientes (11 do sexo masculino), com idade mediana (percentil 25–75, P25–P75) de 2 (2,0–2,8) anos ao diagnóstico. Quinze eram sensíveis a esteroides, e 1 resistente a esteroides e sensível à ciclosporina.A idade mediana na administração do RTX foi 10 (6,3–14,0) anos. Durante um tempo mediano de acompanhamento de 2,5(1,0–3,0) anos, 6 (37,5%) pacientes alcançaram remissão parcial e 7 (43,8%) não tiveram recidivas e não estavam tomando imunossupressor no acompanhamento aos 24 meses. Quanto às complicações,1 paciente apresentou hipogamaglobulinemia persistente. Comparado ao período de12 meses anterior ao RTX, houve diminuição no número mediano de recidivas em 6 e 12 meses [3 (3,0–4,0) vs 0 (0–0,8) e 0,50 (0–1,0), respectivamente; p = 0,001] e na dose diária de esteroides (mg/kg/dia) aos 6, 12 e 24 meses [0,29 (0,15–0,67) >vs [0,10 (0,07–0,13); p = 0,001], [0,12 (0,05–0,22); p = 0,005] e [0,07 (0,04–0,18); p = 0,021], respectivamente. Houve também redução na mediana do escore z do IMC aos 24 meses [2,11 (0,45–3,70) vs 2,93 (2,01–3,98);p = 0,049]. Conclusões: Nossos resultados confirmam a eficácia e segurança do uso de RTX em SN idiopática pediátrica, destacando seus potenciais benefícios cardiometabólicos.

ABSTRACT Objective: This study aimed to translate, and perform a cross-cultural adaptation, and validation of the Vancouver Symptom Score (VSS) for bladder and bowel dysfunction (BBD) for Brazilian children and adolescents Materials and Methods: Six steps were performed for the translation and cross-cultural adaptation: (1) translation, (2) synthesis of translations, (3) back-translation, (4) pre-final version of the translated instrument, (5) pilot test and degree of comprehensibility and (6) elaboration of the Brazilian version of the VSS. For validation, the Brazilian Dysfunctional Voiding Score (DVSS) questionnaire was used. Results: Validation was performed on a sample of 107 children and adolescents with a mean age of 9.2 ± 2.84 years, presenting BBD and 107 without BBD (control group-CG). There was a positive correlation (r = 0.91, 95% CI 0.88 to 0.93, p < 0.0001) between total VSS score and total DVSS score. VSS was higher in patients with BBD (p < 0.0001). The internal consistency estimated by Cronbach's alpha was 0.87 for patients with BBD. The VSS showed excellent diagnostic accuracy in detecting cases, with an area under the ROC curve of 98% (95% CI 0.96 to 0.99, p < 0.001). A cut-off value of >11 points produced a sensitivity of 100% (95% CI 96.4% to 100%) and a specificity of 91.8% (95% CI 85.1% to 95.6%). Conclusion: The translated, cross-culturally adapted, and validated VSS for the Brazilian population is a reliable and valid tool to identify symptoms of BBD in children and adolescents aged five to 16 years, whose first language is Brazilian Portuguese.

RESUMEN Objetivo. Cartografiar los cursos y programas de formación en salud pública ofrecidos en América Latina e identificar estrategias formativas y competencias desarrolladas en la formación regional de salubristas. Métodos. Se realizó una búsqueda en internet para identificar cursos y programas de formación en salud pública ofrecidos por instituciones latinoamericanas. Se recopilaron los siguientes datos: nombre, país y provincia de la institución formadora; nombre del curso; característica de la institución; nivel formativo; modalidad; acreditación; año de la primera oferta; regularidad; y datos de contacto. Luego se analizaron los programas y currículos de las ofertas por nivel formativo a través de técnica de análisis de contenido descriptiva y se identificaron las principales competencias desarrolladas. Resultados. El mapeo permitió identificar de 2 296 ofertas formativas en salud pública en todas las subregiones de América Latina, distribuidas en 29 de los 33 países de la Región. Las ofertas identificadas se presentaban bastante heterogéneas, tanto desde el punto de vista conceptual como programático, y con distribución geográfica desproporcional en los países y entre las subregiones. El análisis de los currículos y contenidos programáticos detectó la necesidad de una mayor vinculación con las funciones esenciales en salud pública. Conclusiones. Se observó la necesidad de adecuar las competencias y habilidades desarrolladas por los cursos y programas a las demandas de los usuarios de los servicios, programas y sistemas de salud regionales, para lograr programas orientados al enfrentamiento de los procesos de determinación socioambiental de la salud y de producción y reproducción de desigualdades.

RESUMO Objetivo. Mapear os cursos e programas de formação em saúde pública oferecidos na América Latina e identificar estratégias de formação e competências desenvolvidas na formação regional de profissionais de saúde pública. Métodos. Foi realizada uma busca na internet para identificar cursos e programas de formação em saúde pública oferecidos por instituições latino-americanas. Foram coletados os seguintes dados: nome, país e estado ou província da instituição formadora; nome do curso; característica da instituição; nível de formação; modalidade; acreditação; ano da primeira turma; regularidade e detalhes de contato. Em seguida, os programas e currículos oferecidos foram analisados por nível de formação, por meio da técnica descritiva de análise de conteúdo, e foram identificadas as principais competências desenvolvidas. Resultados. O mapeamento permitiu identificar 2 296 ofertas de formação em saúde pública em todas as sub-regiões da América Latina, distribuídas em 29 dos 33 países da Região. As ofertas identificadas foram bastante heterogêneas, tanto do ponto de vista conceitual como programático, e com uma distribuição geográfica desproporcional nos países e entre as sub-regiões. A análise dos currículos e conteúdos programáticos detectou a necessidade de um maior vínculo com as funções essenciais da saúde pública. Conclusão. Observou-se a necessidade de adequar as competências e habilidades desenvolvidas pelos cursos e programas às demandas dos usuários dos serviços, programas e sistemas regionais de saúde, a fim de concretizar programas voltados para o enfrentamento dos processos de determinação socioambiental da saúde e de produção e reprodução de desigualdades.

ABSTRACT Objective. Map the public health training courses and programs offered in Latin America, and identify regional training strategies and competencies developed in health workers. Methods. An internet search was conducted to identify public health training courses and programs offered by Latin American institutions. The following data were collected: name, country, and province/state of the training institution; course name; type of institution; educational level; modality; accreditation; year first offered; periodicity; and contact information. The programs and curricula on offer were analyzed by training level using descriptive content analysis, and the main competencies developed were identified. Results. The mapping identified 2 296 public health training opportunities in all Latin American subregions, distributed across 29 of the Region's 33 countries; these were fairly heterogeneous, both conceptually and programmatically, with uneven geographical distribution within and between countries. In the analysis of curricula and programmatic contents, a need for greater linkage with essential public health functions was detected. Conclusion. It is necessary to adapt the competencies and skills developed by the courses and programs to the demands of users of the regional health services, programs, and systems in order to achieve programs aimed at tackling the socio-environmental determinants of health and the production and reproduction of inequalities.

A nutrição durante a gravidez é essencial para a saúde da gestante, o desenvolvimento do bebê e a prevenção de complicações relacionadas à gravidez e ao pós-parto. Este estudo descreveu os fatores associados ao alto consumo de alimentos ultraprocessados entre gestantes. Trata-se de uma coorte prospectiva realizada de fevereiro de 2016 a novembro de 2019, em duas unidades de saúde do Município do Rio de Janeiro, Brasil, que analisou dados de 344 gestantes. A primeira entrevista foi realizada na consulta pré-natal com menos de 20 semanas de gestação, a segunda com 34 semanas de gestação e a terceira dois meses após o parto. A dieta foi avaliada na última entrevista por meio de um questionário de frequência alimentar e os itens alimentares foram classificados de acordo com a classificação NOVA. O percentual de consumo de alimentos ultraprocessados foi calculado em tercis de distribuição, dos quais o terceiro tercil representou o maior consumo. Com base no modelo de análise hierárquica, as associações entre o consumo de alimentos ultraprocessados e variáveis sociodemográficas, de saúde reprodutiva, pré-gestacionais, comportamentais e gestacionais foram investigadas usando um modelo de regressão logística multinomial. Mulheres mais velhas apresentaram menor consumo de alimentos ultraprocessados (OR = 0,33; IC95%: 0,15-0,71). Os fatores de risco foram baixa escolaridade (até sete anos; OR = 5,58; IC95%: 1,62-19,23), histórico de parto anterior (OR = 2,48; IC95%: 1,22-5,04), histórico de dois ou mais partos anteriores (OR = 7,53; IC95%: 3,02-18,76) e ausência de histórico de atividade física regular antes da gestação (OR = 2,40; IC95%: 1,31-4,38). A identificação de fatores de risco e proteção permite o estabelecimento de medidas de controle e o incentivo a práticas saudáveis durante o pré-natal.

La nutrición durante el embarazo es esencial para la salud de la futura madre, el desarrollo del bebé y la prevención de complicaciones relacionadas con el embarazo y el posparto. Este estudio describió los factores asociados con el alto consumo de alimentos ultraprocesados entre las mujeres embarazadas. Se trata de una cohorte prospectiva realizada entre febrero de 2016 y noviembre de 2019, en dos unidades de salud de la ciudad de Río de Janeiro, Brasil, que analizó datos de 344 gestantes. La primera entrevista se realizó en la visita prenatal a las 20 semanas de gestación, la segunda a las 34 semanas de gestación y la tercera dos meses después del parto. La dieta se evaluó en la última entrevista mediante un cuestionario de frecuencia de alimentos y los alimentos se clasificaron de acuerdo con la clasificación NOVA. El porcentaje de consumo de alimentos ultraprocesados se calculó en terciles de distribución, de los cuales el tercer tercil representó el mayor consumo. Con base en el modelo de análisis jerárquico, se investigaron las asociaciones entre el consumo de alimentos ultraprocesados y las variables sociodemográficas, de salud reproductiva, previas al embarazo, conductuales y gestacionales mediante un modelo de regresión logística multinomial. Las mujeres mayores presentaron menor consumo de alimentos ultraprocesados (OR = 0,33; IC95%: 0,15-0,71). Los factores de riesgo fueron bajo nivel educativo (hasta siete años; OR = 5,58; IC95%: 1,62-19,23), antecedentes de parto previo (OR = 2,48; IC95%: 1,22-5,04), antecedentes de dos o más partos previos (OR = 7,53; IC95%: 3,02-18,76) y sin antecedentes de actividad física regular antes del embarazo (OR = 2,40; IC95%: 1,31-4,38). La identificación de factores de riesgo y protección permite el establecimiento de medidas de control y el fomento de prácticas saludables durante la atención prenatal.

Nutrition during pregnancy is essential for the health of the pregnant woman, the development of the fetus, and the prevention of complications related to pregnancy and the postpartum period. This study described the factors associated with high consumption of ultra-processed foods among pregnant women. This prospective cohort study was performed from February 2016 to November 2019 in two health units in the city of Rio de Janeiro, Brazil, with data from 344 pregnant women. The first interview was conducted in the prenatal visit at less than 20 gestational weeks, the second at 34 gestational weeks, and the third at two months postpartum. Diet was assessed in the last interview using a food frequency questionnaire, and food items were classified according to NOVA. The percentage of ultra-processed foods consumption was estimated by tertile distribution, and the third tertile represented the highest consumption. Based on the hierarchical analysis model, the associations between ultra-processed foods consumption and sociodemographic, reproductive health, pregestational, behavioral, and pregnancy variables were assessed using a multinomial logistic regression model. Older women had lower ultra-processed foods consumption (OR = 0.33; 95%CI: 0.15-0.71). Few years of schooling (up to 7 years; OR = 5.58; 95%CI: 1.62-19.23), history of a previous childbirth (OR = 2.48; 95%CI: 1.22-5.04), history of two or more previous childbirths (OR = 7.53; 95%CI: 3.02-18.76), and no history of regular physical activity before pregnancy (OR = 2.40; 95%CI: 1.31-4.38) were risk factors. The identification of risk and protection factors allows for the establishment of control measures and encouragement of healthy practices during prenatal care.

RESUMO OBJETIVO Mapear as ofertas formativas em saúde pública no Brasil e identificar, em seus currículos e planos de estudo, as aproximações entre as competências e habilidades neles desenvolvidas e as funções essenciais de saúde pública. MÉTODOS Estudo descritivo, exploratório, baseado em levantamento e análise de informações disponíveis nos sites de instituições formadoras que ofertam cursos e programas de formação em saúde pública, no Brasil. Os dados foram levantados no âmbito do I Mapeo de Cursos y Programas de Formación en Salud Pública de América Latina. RESULTADOS Foram identificados 1.222 cursos e programas de formação em saúde pública oferecidos no país, com distribuição desigual no território, porém com ocorrência em todas as unidades da federação. Os resultados desvelaram um conjunto de desafios à formação de sanitaristas, organizados em torno do distanciamento entre as ofertas e as demandas formativas em saúde pública (distribuição desigual de cursos, concentração de capacidades formativas nas capitais e em determinadas regiões, entre outros) e do distanciamento entre teorias e práticas formativas (baixa vinculação das competências desenvolvidas com as funções essenciais de saúde pública, predomínio da lógica disciplinar orientada por núcleos de competência profissional, entre outros). CONCLUSÕES A superação desses desafios requer esforços estruturais, políticos e de apropriação tecnológica, ampliando o acesso dos atores da prática a uma formação vinculada com as demandas dos serviços e programas de saúde no país.

ABSTRACT OBJECTIVE To map public health courses and training programs in Brazil and identify, in their curricula and study plans, the level of linkage between the skills and competencies developed by them and the essential public health functions. METHODS Descriptive, exploratory study based on collection and analysis of information available on the websites of educational institutions that offer public health courses and training programs in Brazil. Data was collected within the scope of the I Mapeo de Cursos y Programas de Formación en Salud Pública de América Latina. RESULTS A total of 1,222 public health courses and training programs offered in the country were identified, with unequal territorial distribution but taking place in all federation units. Results revealed a set of challenges to public health professionals’ training, including lack of linkage both between training offer and demand in public health (unequal distribution of courses, concentration of training capacity in capitals and in certain regions, among others), and between theories and training practices (low levels of connection of developed skills and competencies with the essential public health functions, predominance of disciplinary method guided by professional competence centers, among others). CONCLUSIONS Overcoming these challenges requires structural, political, and technological efforts, narrowing the gaps between public health training programs’ availability and the demands of health services and programs in the country.

Abstract Leadership theories developed since the beginning of the last century primarily represent positivist premises, strongly marked by the separation between subject and object, dichotomization, objectivism, quantification, reproduction, and validation. Based on this, we argue that the field needs epistemological approaches that challenge the mainstream of leadership studies. To this end, phenomenology can ground the possibility of understanding leadership as a world phenomenon. In theoretical terms, phenomenology is a return to the acts through which we gain knowledge of objects, thus the proposal to return to the things themselves. This article analyzes the contributions of phenomenology to leadership studies. In order to interact with such identified gaps in leadership studies, we explore the rescue of the lifeworld in the face of objectivism, the reconciliation between subject and object, the attribution of meanings, the epoché, and the change of positioning.

Resumen Las teorías del liderazgo que se han desarrollado desde principios del siglo pasado representan, en su mayoría, premisas positivistas, fuertemente marcadas por la separación entre sujeto y objeto, dicotomización, objetivismo, cuantificación, reproducción y validación. A partir de esto, argumentamos que el campo carece de enfoques epistemológicos que desafíen la corriente principal de los estudios de liderazgo. Para ello, la fenomenología puede sustentar la posibilidad de entender el liderazgo como un fenómeno en el mundo. En términos teóricos, la fenomenología es una vuelta a los actos a través de los cuales se conocen los objetos, de ahí la propuesta de volver a las cosas mismas. Así, el objetivo de este artículo es analizar las contribuciones de la fenomenología a los estudios de liderazgo. Por tanto, para interactuar con tales vacíos identificados en el campo de los estudios de liderazgo, exploramos el rescate del mundo de la vida frente al objetivismo; la reconciliación entre sujeto y objeto, atribución de significados; la epoché y el cambio de posición.

Resumo As teorias de liderança que vêm sendo desenvolvidas desde o início do século passado representam, em sua maioria, premissas positivistas, fortemente marcadas pela separação entre sujeito e objeto, dicotomização, objetivismo, quantificação, reprodução, validação. Com base nisso, argumentamos que o campo carece de abordagens epistemológicas que desafiem o mainstream dos estudos de liderança. Nesse intuito, a fenomenologia pode fundamentar a possibilidade para o entendimento da liderança enquanto um fenômeno no mundo. Em termos teóricos, a fenomenologia é um retorno aos atos por meio dos quais se tem conhecimento dos objetos: por isso a proposta de se voltar às coisas mesmas. Desse modo, o objetivo deste artigo é analisar as contribuições da fenomenologia para os estudos em liderança. Para tanto, de modo a interagir com tais lacunas identificadas no campo de estudos de liderança, exploramos o resgate do mundo-da-vida frente ao objetivismo; à reconciliação entre sujeito e objeto; à atribuição de sentidos; à epoché e à mudança de posicionamento.

ABSTRACT Objective: To verify the association between clinical and sociodemographic factors and time spent sitting in military police. Method: This is a cross-sectional study, with 432 military police officers from Eastern Regional Policing Command units of the Military Police of Bahia de Feira de Santana. Data collection took place from August to December 2022 through Google Forms using the International Physical Activity Questionnaire. Results: Men predominated (82.35%), race/color was black (87.04%), the head of the family had completed higher education (47.69%) and police officers with a partner (81.94%). The risk of time spent sitting ≥ 180 minutes per day was lower in males (IRR < 1). Increasing age was associated with a lower risk of time spent sitting ≥ 180 minutes per day (IRR < 1). Conclusion: Male police officers with more years of experience were less exposed to sedentary behavior. Specific interventions and health policies aimed at combating sedentary behavior become relevant, aiming to promote health and prevent diseases.

RESUMEN Objetivo: Verificar la asociación entre factores clínicos y sociodemográficos y el tiempo de permanencia en la policía militar. Método: Estudio transversal, con 432 policías militares de unidades del Comando de Policía Regional Este de la Policía Militar de Bahía de Feira de Santana. La recolección de datos se realizó de agosto a diciembre de 2022 a través de Google Forms utilizando el Cuestionario Internacional de Actividad Física. Resultados: Predominaron los hombres (82,35%), la raza/color fue negra (87,04%), el jefe de familia tenía estudios superiores (47,69%) y los policías con pareja (81,94%). El riesgo de pasar tiempo sentado ≥ 180 minutos por día fue menor en los hombres (IRR < 1). El aumento de la edad se asoció con un menor riesgo de pasar tiempo sentado ≥ 180 minutos por día (IRR < 1). Conclusión: Los policías varones con más años de experiencia estuvieron menos expuestos al comportamiento sedentario. Cobran relevancia intervenciones y políticas de salud específicas dirigidas a combatir el sedentarismo, con el objetivo de promover la salud y prevenir enfermedades.

RESUMO Objetivo: Verificar a associação entre fatores clínicos e sociodemográficos e o tempo gasto sentado em policiais militares. Método: Estudo transversal, com 432 policiais militares das unidades do Comando de Policiamento Regional Leste da Polícia Militar da Bahia de Feira de Santana. A coleta de dados ocorreu de agosto a dezembro de 2022 através do Google Forms constando o Questionário Internacional de Atividade Física. Resultados: Predominaram homens (82,35%), raça/cor negra (87,04%), nível de escolaridade do chefe da família superior completo (47,69%) e policiais com companheiro(a) (81,94%). O risco do tempo gasto sentado ≥ 180 minutos por dia foi menor no sexo masculino (IRR < 1). O aumento da idade foi associado a menor risco de tempo gasto sentado ≥ 180 minutos por dia (IRR < 1). Conclusão: Policiais do sexo masculino e com mais anos de vida estavam menos expostos ao comportamento sedentário. Intervenções específicas e políticas de saúde voltadas ao combate do comportamento sedentário se tornam relevantes, visando à promoção da saúde e prevenção de agravos.

Abstract Salmonella enterica serovar Heidelberg (SH), often isolated from broiler chicken samples, damages the entire production chain due to its high resistance in the environment. The search for sustainable disinfectant agents has intensified, focusing on the action of essential oils. This work's objective was to evaluate the in vitro, effect of Thymus vulgaris essential oil (TEO) and thymol in SH isolated from broilers, and the cytotoxicity in animal cells. Four different concentrations of TEO (0.1%, 0.2%, 0.4%, and 0.8%, (v/v)) were used against five SH isolates obtained from broilers and ATCC 8326. Thymol was evaluated at the concentrations of 0.023%, 0.047%, 0.071%, and 0.094% (v/v). In vitro antibacterial assays were performed by quantifying viable planktonic cells in the broth microdilution test. The MTT technique was used to assess the cytotoxicity in IEC-6 intestinal cells and NCTC fibroblasts. IC50 was assessed testing the concentrations of 0.0625%, 0.125%, 0.25%, and 0.5% of TEO and thymol in 24 hours. The bacterial activity was observed from the 0.2% concentration of TEO since no colony-forming units were observed. Thymol in the concentration of 0.094% controlled 83.33% of the bacteria. TEO presented an IC50 of 0.14% and 1.22%, while the IC50 for thymol was 0.068% and 0.001% for the IEC-6 and NCTC cells, respectively. On the other hand, TEO showed low cytotoxicity in fibroblasts and a potential action capacity to eliminate SH strains in vitro.

Abstract Infection disease caused by nontuberculous mycobacteria (NTM) have been increasingly reported and often manifest with the same symptoms of tuberculosis (TB). The identification of the causative agent is fundamental for the determination of an appropriate therapy, since each species of mycobacteria requires a specific treatment. Thus, rapid and accurate tests to identify mycobacteria of medical interest, such as Mycobacterium tuberculosis complex (MTBC) and Mycobacterium avium complex (MAC), are necessary in the clinical routine. The present study evaluated an in-house multiplex PCR to detect, in reference and clinical strains, the genus Mycobacterium and also M. tuberculosis and MAC. To identify the Mycobacterium genus, M. tuberculosis and MAC, it was amplified a fragment of hsp65 gene, esat-6 gene, and the internal transcribed spacer between the 16S and 23S rRNA genes, respectively. In total, 87 mycobacteria strains were used, being 10 reference and 77 clinical strains, previously identified as MTBC (n = 66), M. avium (n = 8) or other NTM specie (n = 13). The hsp65 gene fragment was amplified for all mycobacteria strains evaluated (87/87). This multiplex PCR presented sensitivity of 100% and specificity of 95.2% for M. tuberculosis detection, and sensitivity of 100% and specificity of 100% for MAC detection. The multiplex PCR evaluated is an important tool for the differentiation between M. tuberculosis and NTM, as well as for the identification of MAC, a complex composed by species with high prevalence in the world.

Abstract Background Human Papillomavirus (HPV) is the main etiological factor for the development of cervical cancer. HPV 18 is the second most frequent type, accounting for up to 65% of all cases. HPV intratypic variation may influence the potential for progression to invasive cancer. The aim of this study was to evaluate the prevalence of human papillomavirus 18 intratypic variants in cervical cancer samples from women in the state of Maranhão, Brazil. Methods The study included 118 women over 18 years of age with a diagnosis of cervical cancer. Tumor fragments were collected and subjected to DNA extraction and Polymerase Chain Reaction (PCR) for HPV detection using the PGMY09/11 and GP+5/6 primers. Positive samples were submitted to automated sequencing for viral genotyping. To determine the HPV 18 lineages, positive samples were submitted to PCR, using specific primers to amplify the LCR and E6 regions of HPV 18 virus. Results HPV was present in 88 women (73.3%). Of those, 48 (54%) were HPV 16, the most prevalent, followed by 12 (13.6%) HPV 18. Histologically, squamous cell carcinoma was predominant (79.1%). Among the HPV 18 variants identified, 10 (80%) belonged to lineage A, and sublineages A1, A2, A3, and A4. Two (29%) HPV 18 B variant was also detected, with the sublineages B1 and B2. In this study, the C variant was not found. There was no statistically significant association between the HPV 18 lineages found and sociodemographic and lifestyle variables (p > 0.05). Conclusions A higher frequency of HPV 16 and 18 were found in women with cervical cancer in the state of Maranhão, Brazil, with a high prevalence of the lineage A among women with HPV 18.

ABSTRACT Objective: to understand what undergraduate Nursing students from a university in southern Brazil know about trans people. Method: a descriptive study with a qualitative approach based on interviews conducted with 19 undergraduate Nursing students enrolled from the eighth to the tenth period of the course. Data collection took place in October and November 2021. Three thematic categories emerged from the thematic analysis, namely: the undergraduate course has to speak about this!; I don't know what it is about; and What is known and said about trans people. Results: the students showed insecurity in the care of trans people because this content is not regularly taught in the curriculum. They denote not knowing how to welcome, care for, respect specificities and treat them by their social name, as well as difficulties taking care of this population group, both in the present time and in the future. In addition to that, few of them had the opportunity of undergoing this experience in the academic practice fields. The content is taught in a single academic discipline that deals with sexuality in general. Conclusion: lack of knowledge about sexual diversity is a limiting factor in the health care provided to this population group. Certain weakness is identified in the teaching-learning process when not relating the reality of the trans population to their health demands.

RESUMEN Objetivo: comprender el conocimiento de los estudiantes de grado de Enfermería de una universidad del sur de Brasil en relación a las personas trans. Método: estudio descriptivo de enfoque cualitativo a partir de entrevistas realizadas con 19 estudiantes de grado de Enfermería inscritos en el octavo a décimo período del curso. La recolección de datos tuvo lugar en octubre y noviembre de 2021. Surgieron 3 categorías del análisis temático: ¡En la carrera de grado se debe hablar de esto!; No sé de qué se trata; y Lo que se sabe y dice sobre las personas trans. Resultados: los estudiantes se muestran inseguros en la atención de las personas trans debido a que este contenido no se ofrece regularmente en el plan de estudio. Denotan desconocimiento sobre como recibir a las personas trans, atenderlas, respetar sus especificidades y tratarlas por su nombre social, además de dificultades en el cuidado de este grupo poblacional, tanto en el presente como en el futuro. Además, pocos participantes tuvieron la oportunidad de vivir esta experiencia en los campos de práctica académica. El contenido solo se dicta en una disciplina que trata el tema dela sexualidad en general. Conclusión: el desconocimiento acerca de la diversidad sexual es un factor limitante en la atención de la salud que se proporciona a este grupo poblacional. Se identifica cierta fragilidad en el proceso de enseñanza-aprendizaje ya que no se relaciona la realidad de la población trans con sus necesidades en materia de salud.

RESUMO Objetivo: compreender o conhecimento dos estudantes de graduação de enfermagem em uma universidade do sul do Brasil no tocante às pessoas trans. Método: estudo descritivo com abordagem qualitativa a partir de entrevistas realizadas com 19 estudantes de graduação em enfermagem matriculados do oitavo ao décimo período do curso. A coleta de dados ocorreu nos meses de outubro e novembro de 2021. Da análise temática emergiram 3 categorias: o curso de graduação precisa falar disso!; eu não sei do que se trata; e o que se sabe e se diz sobre as pessoas trans. Resultados: os estudantes demonstram insegurança no cuidado das pessoas trans devido ao não oferecimento regular deste conteúdo no currículo. Denotam desconhecimento de como acolher, cuidar, respeitar as especificidades, tratar pelo nome social, e também dificuldades em cuidar desta população no presente e no futuro. Além disso, poucos tiveram a oportunidade de vivenciar esta experiência nos campos de prática acadêmica. O conteúdo é oferecido em uma única disciplina que trata da sexualidade em geral. Conclusão: o desconhecimento acerca da diversidade sexual é um fator limitador na assistência à saúde a esta população. Identifica-se uma fragilidade no ensino-aprendizagem ao não relacionar a realidade da população trans com suas demandas de saúde.

Abstract In this research, aqueous and ethanolic extracts from Justicia pectoralis Jacq and Croton Jacobinensis Baill were characterized. The UPLC-QTOF-MSE analysis was performed on the extracts identified, predominantly, flavonoids, tannins and acids. The extracts did not indicate toxicity in human epithelial cells. C. jacobinensis presented a concentration of phenolics 60.5% higher than J. pectoralis in all scenarios evaluated and, for both samples, the hydroalcoholic extract at 70% exhibited the best efficiency in the extraction (14501.3 and 32521.5 mg GAE 100 g-1 for J. pectoralis and C. jacobinensis, respectively). The antioxidant activity presented a positive correlation with the concentration of phenolics, being 1.186,1 and 1.507,9 μM of Trolox for J. pectoralis and C. jacobinensis at 70% of ethanol; however, it was not verified statistical difference between the ethanolic solutions (p < 0.05). The antimicrobial activity of J. pectoralis extracts was highlighted once was the most effective against gram-positive bacteria. The results suggest that both J. pectoralis and C. jacobinensis extracts present the potential to be applied as natural additives due to their antioxidant and antimicrobial activity and safety. Thus, it is suggesting the development of studies that could investigate the interaction of these plant extracts with food matrices is required.

ABSTRACT Background: Brazil has one of the highest numbers of COVID-19 cases and deaths. Rio Grande do Sul (RS) in southern Brazil is one of the leading states in terms of case numbers. As part of the national public health network, the State Central Laboratory (LACEN-RS) changed its routine in 2020 to focus on the diagnosis of COVID-19. This study evaluated the laboratory surveillance of COVID-19 suspected cases analyzed at the LACEN-RS in 2020. Methods: Viral detection was performed using RT-qPCR in samples from patients with respiratory infection who met the study criteria. Viral RNA was isolated using commercial manual kits or automated extractors, and SARS-CoV-2 RT-qPCR was performed using the Bio-Manguinhos/Rio de Janeiro, IBMP/Paraná, or Allplex 2019-nCoV assay. In total, 360 representative SARS-CoV-2 samples were sequenced using the Illumina platform. Results: In total, 31,197 of 107,578 (positivity rate = 29%) tested positive for SARS-CoV-2. The number of RT-qPCR tests performed per month followed the COVID-19 epidemic curve observed for the state, with peaks in July-August and December. Females accounted for 63% of the samples, whereas the positivity rate was higher among males (33.1% males vs. 26.5% females). The positivity rate was higher in adults aged 50-79 years compared to the overall positivity rate. The majority of cases were observed in the capital, Porto Alegre, and the metropolitan region. Ten distinct lineages were identified, with B.1.1.28, B.1.1.33, and P.2 being the most frequent. Conclusions: Here, we describe laboratory surveillance of COVID-19 to identify priorities for epidemiological surveillance actions in RS.